CN104258276A - Traditional Chinese medicine composition for treating non-alcoholic fatty liver disease and preparation method of traditional Chinese medicine composition - Google Patents
Traditional Chinese medicine composition for treating non-alcoholic fatty liver disease and preparation method of traditional Chinese medicine composition Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating non-alcoholic fatty liver disease and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following components: fructus aurantii, semen plantaginis, root of common peony, chicken's gizzard-membrane, rhizoma curcumae longae, ginseng root, rhizoma anemarrhenae, astragalus membranaceus, Chinese yam, dried rehmannia root, radix scrophulariae, radix glehniae, radix ophiopogonis, radix polygonati officinalis, rhizoma polygonati, radix trichosanthis, pulp of dogwood fruit, fruit of Chinese wolfberry, glossy privet fruit, herba epimedii, bark of tree peony root, poria, monkshood, rhizoma alismatis and schisandra chinensis. The preparation method of the traditional Chinese medicine composition comprises the following steps: getting the crude drugs of the traditional Chinese medicine, uniformly mixing and crushing into crude powder, performing supercritical CO2 extraction, adsorbing extracting liquor by virtue of macroporous adsorption resin, eluting by virtue of alcohol aqueous liquid, filtering eluate by virtue of an ultrafiltration membrane, concentrating and drying to obtain the traditional Chinese medicine composition. The traditional Chinese medicine composition for treating non-alcoholic fatty liver disease can be used for preventing and treating hepatic steatosis, protecting the liver function, and presenting treatment effects with a plurality of target spots and a plurality of ways, and is free of toxic and side effect.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease and preparation method thereof.
Background technology
Fatty liver is that the liver fat sample caused due to Different types of etiopathogenises or disease becomes, and when the accumulation of hepatocyte inner lipid is more than 5%, or when the hepatocyte generation fat of the upper per unit area more than 1/3 of histology becomes, is referred to as fatty liver.Fatty liver comprises alcoholic fatty liver (alcoholic fatty liver disease, AFLD) and the large class of non-alcoholic fatty liver disease (non-alcoholic fatty liver disease, NAFLD) two.Wherein, non-alcoholic fatty liver disease is a kind of without excessive drinking history, the clinical pathology syndrome being principal character with hepatic parenchymal cells steatosis, necrosis, cell infiltration and lipopexia etc.Spectrum of disease comprises nonalcoholic fatty liver (non-alcoholic simple fatty liver, NAFL), non-alcoholic stellato-hepatitis (non-alcoholic steatohepatitis.NASH) and related liver cirrhosis thereof and hepatocellular carcinoma.Along with the change with dietary structure that improves constantly of people's living standard, the prevalence of non-alcoholic fatty liver disease increases sharply, and present the trend of becoming younger, current non-alcoholic fatty liver disease has become the second largest hepatopathy that China is only second to viral hepatitis, also be acknowledged as the chronic hepatopathy problem that China is more and more serious, the health of the mankind in serious harm.Because its clinical onset has universality and invisible more, and the hazardness of pathological changes is larger, therefore non-alcoholic fatty liver disease has become a kind of disease with potential hazard in China and even the whole world, be one of important public health problem in the 21 century whole world, therefore the research of non-alcoholic fatty liver disease preventive and therapeutic effect had great significance.
Modern medicine Drug treatment non-alcoholic fatty liver disease mainly in blood fat reducing, protect the liver and improve in insulin resistant etc., although the research of existing small sample finds really effective to improving objective indicator, but still there is its place of being short of (as: though the use of fat-reducing medicament can reduce blood fat, but the fat deposited in liver can not well be removed, and long-term, high-dose uses these medicines easily hepatotoxicity to occur), and the improvement of self symptom of effect and patient of Drug therapy does not conform to sometimes.Western medicine in effective, safety, reasonable price, improve in symptom etc. and all certain constraint produced to its treatment.
In recent years, Chinese medicine shows gratifying curative effect in treatment non-alcoholic fatty liver disease.Clinical practice and pharmacodynamic experiment show that Chinese medicine can adjusting blood lipid, and control hepatic steatosis, liver function protecting, present Mutiple Targets, multipath response to treatment, and toxic and side effects is less.As CN201410126690.5 discloses a kind of by marigold flower, Fructus Akebiae, Rhizoma Polygoni Cuspidati, Pericarpium Citri Reticulatae Viride, Stigma Maydis, Endoconcha Sepiae, the Chinese medicine of the treatment fatty liver that Herba Camptosori Sibirici is made, CN 201310128523.X discloses a kind of Chinese medicine for the treatment of fatty liver, adopts Rhizoma Curcumae 9 parts, Herba Galii Teneri 6 parts, Pericarpium Citri Reticulatae 15 parts, Radix Bupleuri 6 parts, Flos Pruni mume 8 parts, 7 parts, Rhizoma Curcumae Longae, Rhizoma Imperatae 12 parts, 10 parts, Poria, the Rhizoma Pinelliae 9 parts, Radix Aneurolepidii 6 parts formulated.Although these medicines have certain effect to treatment fatty liver tool, still there is a lot of defect, such as take effect slowly, curative effect not significantly, and is carried, use the shortcomings such as inconvenience.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and a kind of Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease and preparation method thereof is provided; this Chinese medicine composition can prevent and treat hepatic steatosis, liver function protecting; present Mutiple Targets, multipath response to treatment, and have no side effect
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 2 ~ 6 parts, Semen Plantaginis 1 ~ 7 part, Radix Paeoniae Rubra 3 ~ 9 parts, Endothelium Corneum Gigeriae Galli 4 ~ 9 parts, 2 ~ 7 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 1 ~ 9 part, the Rhizoma Anemarrhenae 3 ~ 10 parts, the Radix Astragali 2 ~ 9 parts, Rhizoma Dioscoreae 1 ~ 7 part, 4 ~ 12 parts, the Radix Rehmanniae, Radix Scrophulariae 5 ~ 10 parts, Radix Glehniae 1 ~ 7 part, Radix Ophiopogonis 3 ~ 8 parts, Rhizoma Polygonati Odorati 2 ~ 9 parts, Rhizoma Polygonati 3 ~ 8 parts, Radix Trichosanthis 2 ~ 7 parts, Fructus Corni 4 ~ 10 parts, Fructus Lycii 1 ~ 8 part, Fructus Ligustri Lucidi 2 ~ 9 parts, Herba Epimedii 1 ~ 7 part, Cortex Moutan 3 ~ 10 parts, PORIA from Yunnan of China 3 ~ 8 parts, Radix Aconiti Lateralis Preparata 2 ~ 9 parts, Rhizoma Alismatis 4 ~ 10 parts, Fructus Schisandrae Chinensis 2 ~ 7 parts.
Fructus Aurantii: the effect with the chest stuffiness relieving of regulating the flow of vital energy, the stagnant removing food stagnancy of row.Cure mainly: septum pectorale feeling of fullness; Distending pain over the hypochondrium; Accumulation of food in the stomach and intes tine due to indigestion; Distension and fullness in the abdomen; Heavy after dysentery; Proctoptosis; Uterine prolapse.
Semen Plantaginis: there is clearing away heat and promoting diuresis, eliminating dampness by diuresis antidiarrheal, improving eyesight, the effect of eliminating the phlegm.Cure mainly: dysuria; Stranguria with turbid discharge leukorrhagia; Edema distension; Heat-damp in summer dysentery; Conjunctival congestion is hidden from view; Expectorant heat syndrome cough is breathed heavily.
Radix Paeoniae Rubra: the effect with clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling.Cure mainly: maculae caused by violent heat pathogen; Hematemesis and epistaxis; Discharging fresh blood stool; Conjunctival congestion and swelling pain; Carbuncle skin infection; Amenorrhea; Dysmenorrhea; Metrorrhagia and leukorrhagia stranguria with turbid discharge; The stagnant hypochondriac pain of the stasis of blood; Hernia abdominal mass is gathered; Injury from falling down.
Endothelium Corneum Gigeriae Galli: the effect with strengthening the spleen to promote digestion, arresting seminal emission, Xiao Disorder fossil.Cure mainly: dyspepsia; Eating accumulation; Vomiting regurgitation; To have loose bowels dysentery; Infantile malnutrition; Seminal emission; The enuresis; Frequent micturition; Urinary system calculus and cholelithiasis; Mass in the abdomen amenorrhea; Sore throat tonsillitis; Ulcerative gingivitis aphtha.
Rhizoma Curcumae Longae: the effect with removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia.Cure mainly: all cards of stagnation of QI and blood; Born of the same parents' abdomen hypochondriac pain; Dysmenorrhes; Amenorrhea; The stagnant stomachache of the stasis of blood in puerperal; Rheumatic arthralgia; Traumatic injury; Carbuncle.
The Rhizoma Anemarrhenae: the effect with clearing away heat-fire, nourshing Yin and drynsessmoistening prescription, relieving restlessness of quenching the thirst.Cure mainly: epidemic febrile disease; High hot excessive thirst; Cough and asthma; Cough caused by dryness; Constipation; Osteopyrexia and fever; Restlessness of asrhenia type and insomnia; To quench one's thirst stranguria with turbid discharge.
The Radix Astragali: the effect with invigorating QI to consolidate the body surface resistance, diuresis, expelling pus and toxin by strengthening QI, promoting tissue regeneration and ulcer healing.Cure mainly: control cardiopalmus of breathing hard; Asthenia; Weak; Spontaneous perspiration; Night sweat; Chronic diarrhea; Proctoptosis; Uterine prolapse; Body is impractical swollen; Chronic nephritis; Carbuncle difficulty is burst; Or burst do not hold back for a long time.
Rhizoma Dioscoreae: have spleen reinforcing, support lung, reinforce the kidney, benefit essence effect.Cure mainly: diarrhea due to hypofunction of the spleen; Lack of appetite edema; Cough and asthma due to lung deficiency; Quench one's thirst; Seminal emission; Leukorrhagia; To suffer from a deficiency of the kidney frequent micturition; Carbuncle is controlled in external; Scrofula.
The Radix Rehmanniae: there is nourishing YIN and clearing away heat, effect that removing heat from blood is enriched blood.Cure mainly: calentura excessive thirst; Interior-heat is quenched one's thirst; Hectic fever due to YIN-deficiency consumptive fever; Epidemic febrile disease sends out speckle; Haematemesis caused by heat in blood; Metrorrhagia; Hematuria; Have blood in stool; Blood deficiency and yellow complexion; Dizzy cardiopalmus; Insufficiency of blood amenorrhea.
Radix Scrophulariae: the effect with clearing away heat and cooling blood, nourishing YIN to lower pathogenic fire, detoxicating and resolving stagnation of pathogens.Cure mainly: warm febrile disease and nutrient blood; Fever of the body; Excessive thirst; Crimson tongue; Send out speckle; Hectic fever due to YIN-deficiency chronic cough; Fidgets due to deficiency is awake not; Constipation is hindered in Tianjin; Order is puckery dim-sighted; Throat larynx swells and ache; Scrofula sucutaneous nodule; Skin sore.
Radix Glehniae: the effect with nourishing YIN and clearing away lung-heat, reinforcing stomach reg fluid.Cure mainly: dryness of the lung dry cough; Asthenia hemoptysis; Stomach level is not enough; Tianjin wound is done.
Radix Ophiopogonis: the effect with YIN nourishing and the production of body fluid promoting.Cure mainly: lung-dryness syndrome due to deficiency of YIN; Cough with ropy sputum; Deficiency of stomach-Yin; Dryness of the mouth and throat; Dryness of the intestine constipation.
Rhizoma Polygonati Odorati: the effect with nourishing YIN and moistening the lung, nourishing the stomach to promote the production of body fluid.Cure mainly: cough caused by dryness; Chronic cough; The dry throat and mouth of calentura cloudy liquid consumption wound; Interior-heat is quenched one's thirst; Deficiency of YIN diseases caused by exogenous pathogenic factor; Dizzy dizzy; Muscle arteries and veins spasm and pain.
Rhizoma Polygonati: the effect with nourishing YIN and moistening the lung, invigorating the spleen and replenishing QI, Zishen fill fine.Cure mainly: deficiency of YIN chronic cough; Xeropulmonary cough; Insufficiency of the spleen weak; Lack of appetite xerostomia; Quench one's thirst; Deficiency of the kidney soreness of the waist and knees; Impotence and seminal emission; Tinnitus order is dark; Early whitening of beard and hair; Body void is won thin; Wind favus disease.
Radix Trichosanthis: there is clearing away heat and promoting production of body fluid, moistening the lung and resolving phlegm, detumescence arrange dense effect.Cure mainly: calentura is thirsty; To quench one's thirst polydipsia; Lung-heat type cough; Sore swollen toxin.
Fructus Corni: there is liver and kidney tonifying, the solid de-effect of convergence.Cure mainly: waist is had a dizzy spell; Deafness and tinnitus; Soreness of the waist and knees; Xian essence spermatorrhea; Frequent micturition; Incessant sweating due to debility; Woman uterine bleeding.
Fructus Lycii: the effect with nourishing the liver, nourishing kidney, lung moistening.Cure mainly: caused by liver and kidney deficiency; Have a dizzy spell; Visual one clear; Soreness of the waist and knees; The expensive essence of sexual impotence; Cough due to consumptive disease; To quench one's thirst taking fluids profusely.
Fructus Ligustri Lucidi: the effect with liver and kidney tonifying, clearind deficient heat, improving eyesight.Cure mainly: Light-headedness; Soreness of the waist and knees; Seminal emission; Tinnitus; Early whitening of beard and hair, osteopyrexia and fever; Poor vision.
Herba Epimedii: the effect with kidney invigorating and YANG supporting, expelling wind and removing dampness, strong muscle key bone.Cure mainly: impotence and seminal emission; Cold caused by deficiency is sterile; Frequent micturition loses Chu; Dyspnea and cough due to deficiency of the kidney; Soreness of the waist and knees; Rheumatic arthralgia; Hemiplegia; Extremity are meciless.
Cortex Moutan: the effect with heat clearing away, promoting blood circulation to remove blood stasis.Cure mainly: the epidemic febrile disease pathogenic heat attacking blood system in febrile diseases; Send out speckle; Tell nosebleed; The pathogenic bacteria later stage brimful of tears, hot Fu Yin divided heating; Deficiency of YIN osteopyrexia and fever; Amenorrhea due to stagnation of blood; Dysmenorrhea; Carbuncle sore tumefacting virus; Fall and flutter the pain of injury; Beriberoid pyretic arthralgia.
As the further improvement of foregoing invention, the described Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 3 ~ 5 parts, Semen Plantaginis 2 ~ 6 parts, Radix Paeoniae Rubra 4 ~ 7 parts, Endothelium Corneum Gigeriae Galli 5 ~ 8 parts, 3 ~ 6 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 3 ~ 8 parts, the Rhizoma Anemarrhenae 5 ~ 9 parts, the Radix Astragali 4 ~ 7 parts, Rhizoma Dioscoreae 3 ~ 6 parts, 6 ~ 10 parts, the Radix Rehmanniae, Radix Scrophulariae 6 ~ 9 parts, Radix Glehniae 2 ~ 6 parts, Radix Ophiopogonis 4 ~ 7 parts, Rhizoma Polygonati Odorati 3 ~ 6 parts, Rhizoma Polygonati 4 ~ 7 parts, Radix Trichosanthis 3 ~ 6 parts, Fructus Corni 5 ~ 8 parts, Fructus Lycii 2 ~ 7 parts, Fructus Ligustri Lucidi 4 ~ 8 parts, Herba Epimedii 2 ~ 6 parts, Cortex Moutan 5 ~ 9 parts, PORIA from Yunnan of China 4 ~ 7 parts, Radix Aconiti Lateralis Preparata 3 ~ 8 parts, Rhizoma Alismatis 5 ~ 9 parts, Fructus Schisandrae Chinensis 3 ~ 6 parts.
As the further improvement of foregoing invention, described in be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 4 parts, Semen Plantaginis 3 parts, Radix Paeoniae Rubra 6 parts, Endothelium Corneum Gigeriae Galli 7 parts, 5 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 5 parts, the Rhizoma Anemarrhenae 8 parts, the Radix Astragali 6 parts, Rhizoma Dioscoreae 4 parts, 9 parts, the Radix Rehmanniae, Radix Scrophulariae 8 parts, Radix Glehniae 5 parts, Radix Ophiopogonis 6 parts, Rhizoma Polygonati Odorati 5 parts, Rhizoma Polygonati 5 parts, Radix Trichosanthis 4 parts, Fructus Corni 7 parts, Fructus Lycii 6 parts, Fructus Ligustri Lucidi 5 parts, Herba Epimedii 4 parts, Cortex Moutan 8 parts, PORIA from Yunnan of China 5 parts, Radix Aconiti Lateralis Preparata 5 parts, Rhizoma Alismatis 8 parts, Fructus Schisandrae Chinensis 5 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, collects extract;
Step 2, adsorbs step 1 gained extract through macroporous adsorbent resin, then carries out eluting with ethanol water, obtain eluent;
Step 3, by step 2 gained eluent after ultrafiltration membrance filter, concentrated, dry, obtain Chinese medicine composition.
As the further improvement of foregoing invention, supercritical CO in step 1
2the pressure of extraction is 15 ~ 40MPa, and temperature is 35 ~ 50 DEG C, and extraction time is 2.5 ~ 4h.
As the further improvement of foregoing invention, macroporous adsorbent resin described in step 2 is selected from AB-8, NKA, HDP-100, HDP-722 or HDP-750.
As the further improvement of foregoing invention, in step 2, the concentration of ethanol water is 70 ~ 80wt.%.
As the further improvement of foregoing invention, the molecular cut off of ultrafilter membrane described in step 3 is 100000 ~ 200000Da.
As the further improvement of foregoing invention, in step 3, drying mode is lyophilization or spraying dry.
The Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease of the present invention can make acceptable peroral dosage form clinically, as capsule, tablet, granule, pill, oral liquid etc. with the pharmaceutics adjuvant accepted of attending class by tradition or modern production process.Excipient substance used can be starch, dextrin, microcrystalline Cellulose, gelatin, sucrose, magnesium stearate etc.Need long-term prescription clinically, it is convenient to make solid preparation storage, and patient takes facility, also more meeting the market requirement.Preferably acute is capsule, granule and tablet.
The Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease provided by the invention, can prevent and treat hepatic steatosis, liver function protecting, presents Mutiple Targets, multipath response to treatment, and has no side effect.
Detailed description of the invention
Embodiment 1
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 2 parts, Semen Plantaginis 1 part, Radix Paeoniae Rubra 3 parts, Endothelium Corneum Gigeriae Galli 4 parts, 2 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 1 part, the Rhizoma Anemarrhenae 3 parts, the Radix Astragali 2 parts, Rhizoma Dioscoreae 1 part, 4 parts, the Radix Rehmanniae, Radix Scrophulariae 5 parts, Radix Glehniae 1 part, Radix Ophiopogonis 3 parts, Rhizoma Polygonati Odorati 2 parts, Rhizoma Polygonati 3 parts, Radix Trichosanthis 2 parts, Fructus Corni 4 parts, Fructus Lycii 1 part, Fructus Ligustri Lucidi 2 parts, Herba Epimedii 1 part, Cortex Moutan 3 parts, PORIA from Yunnan of China 3 parts, Radix Aconiti Lateralis Preparata 2 parts, Rhizoma Alismatis 4 parts, Fructus Schisandrae Chinensis 2 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, supercritical CO
2the pressure of extraction is 15MPa, and temperature is 35 DEG C, and extraction time is 2.5h, collects extract;
Step 2, adsorbs step 1 gained extract through AB-8 macroporous adsorbent resin, then carries out eluting with the ethanol water that concentration is 70wt.%, obtains eluent;
Step 3, by step 2 gained eluent after the ultrafiltration membrance filter that molecular cut off is 100000Da, concentrated, lyophilization, obtains Chinese medicine composition.
Embodiment 2
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 3 parts, Semen Plantaginis 2 parts, Radix Paeoniae Rubra 4 parts, Endothelium Corneum Gigeriae Galli 5 parts, 3 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 3 parts, the Rhizoma Anemarrhenae 5 parts, the Radix Astragali 4 parts, Rhizoma Dioscoreae 3 parts, 6 parts, the Radix Rehmanniae, Radix Scrophulariae 6 parts, Radix Glehniae 2 parts, Radix Ophiopogonis 4 parts, Rhizoma Polygonati Odorati 3 parts, Rhizoma Polygonati 4 parts, Radix Trichosanthis 3 parts, Fructus Corni 5 parts, Fructus Lycii 2 parts, Fructus Ligustri Lucidi 4 parts, Herba Epimedii 2 parts, Cortex Moutan 5 parts, PORIA from Yunnan of China 4 parts, Radix Aconiti Lateralis Preparata 3 parts, Rhizoma Alismatis 5 parts, Fructus Schisandrae Chinensis 3 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, supercritical CO
2the pressure of extraction is 25MPa, and temperature is 40 DEG C, and extraction time is 3h, collects extract;
Step 2, adsorbs step 1 gained extract through HDP-100 macroporous adsorbent resin, then carries out eluting with the ethanol water that concentration is 75wt.%, obtains eluent;
Step 3, by step 2 gained eluent after the ultrafiltration membrance filter that molecular cut off is 150000Da, concentrated, lyophilization, obtains Chinese medicine composition.
Embodiment 3
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 4 parts, Semen Plantaginis 3 parts, Radix Paeoniae Rubra 6 parts, Endothelium Corneum Gigeriae Galli 7 parts, 5 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 5 parts, the Rhizoma Anemarrhenae 8 parts, the Radix Astragali 6 parts, Rhizoma Dioscoreae 4 parts, 9 parts, the Radix Rehmanniae, Radix Scrophulariae 8 parts, Radix Glehniae 5 parts, Radix Ophiopogonis 6 parts, Rhizoma Polygonati Odorati 5 parts, Rhizoma Polygonati 5 parts, Radix Trichosanthis 4 parts, Fructus Corni 7 parts, Fructus Lycii 6 parts, Fructus Ligustri Lucidi 5 parts, Herba Epimedii 4 parts, Cortex Moutan 8 parts, PORIA from Yunnan of China 5 parts, Radix Aconiti Lateralis Preparata 5 parts, Rhizoma Alismatis 8 parts, Fructus Schisandrae Chinensis 5 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, supercritical CO
2the pressure of extraction is 25MPa, and temperature is 40 DEG C, and extraction time is 3h, collects extract;
Step 2, adsorbs step 1 gained extract through NKA macroporous adsorbent resin, then carries out eluting with the ethanol water that concentration is 75wt.%, obtains eluent;
Step 3, by step 2 gained eluent after the ultrafiltration membrance filter that molecular cut off is 150000Da, concentrated, lyophilization, obtains Chinese medicine composition.
Embodiment 4
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 5 parts, Semen Plantaginis 6 parts, Radix Paeoniae Rubra 7 parts, Endothelium Corneum Gigeriae Galli 8 parts, 6 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 8 parts, the Rhizoma Anemarrhenae 9 parts, the Radix Astragali 7 parts, Rhizoma Dioscoreae 6 parts, 10 parts, the Radix Rehmanniae, Radix Scrophulariae 9 parts, Radix Glehniae 6 parts, Radix Ophiopogonis 7 parts, Rhizoma Polygonati Odorati 6 parts, Rhizoma Polygonati 7 parts, Radix Trichosanthis 6 parts, Fructus Corni 8 parts, Fructus Lycii 7 parts, Fructus Ligustri Lucidi 8 parts, Herba Epimedii 6 parts, Cortex Moutan 9 parts, PORIA from Yunnan of China 7 parts, Radix Aconiti Lateralis Preparata 8 parts, Rhizoma Alismatis 9 parts, Fructus Schisandrae Chinensis 6 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, supercritical CO
2the pressure of extraction is 25MPa, and temperature is 40 DEG C, and extraction time is 3h, collects extract;
Step 2, adsorbs step 1 gained extract through HDP-722 macroporous adsorbent resin, then carries out eluting with the ethanol water that concentration is 75wt.%, obtains eluent;
Step 3, by step 2 gained eluent after the ultrafiltration membrance filter that molecular cut off is 150000Da, concentrated, lyophilization, obtains Chinese medicine composition.
Embodiment 5
Be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 6 parts, Semen Plantaginis 7 parts, Radix Paeoniae Rubra 9 parts, Endothelium Corneum Gigeriae Galli 9 parts, 7 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 9 parts, the Rhizoma Anemarrhenae 10 parts, the Radix Astragali 9 parts, Rhizoma Dioscoreae 7 parts, 12 parts, the Radix Rehmanniae, Radix Scrophulariae 10 parts, Radix Glehniae 7 parts, Radix Ophiopogonis 8 parts, Rhizoma Polygonati Odorati 9 parts, Rhizoma Polygonati 8 parts, Radix Trichosanthis 7 parts, Fructus Corni 10 parts, Fructus Lycii 8 parts, Fructus Ligustri Lucidi 9 parts, Herba Epimedii 7 parts, Cortex Moutan 10 parts, PORIA from Yunnan of China 8 parts, Radix Aconiti Lateralis Preparata 9 parts, Rhizoma Alismatis 10 parts, Fructus Schisandrae Chinensis 7 parts.
The above-mentioned preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprises the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, supercritical CO
2the pressure of extraction is 40MPa, and temperature is 50 DEG C, and extraction time is 4h, collects extract;
Step 2, adsorbs step 1 gained extract through HDP-750 macroporous adsorbent resin, then carries out eluting with the ethanol water that concentration is 80wt.%, obtains eluent;
Step 3, by step 2 gained eluent after the ultrafiltration membrance filter that molecular cut off is 200000Da, concentrated, lyophilization, obtains Chinese medicine composition.
Experimental example 1 toxicology test
Acute toxicity test
Animal: Kunming mouse, body weight 21 ~ 24g, male and female half and half.
LD50 calculates: adopt improvement karber's method, mice is divided into 5 groups at random, often organize 10, male and female half and half, Chinese medicine composition adding distil water embodiment 1 prepared dissolves, and is made into Cmax, by the administration of mice maximum permission capacity, institute is followed successively by 18,14.4 to dosage by crude drug amount, 11.5,9.2,7.4 (g/kg), in animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5mL, observes animal dead situation.
Maximum tolerated dose measures (MTD value): get mice 20, each 10 of male and female.Chinese medicine composition adding distil water is dissolved, is made into maximum concentration, by the maximum tolerated dose of animal, feed device and can twitch to inject to fill with and be as the criterion.In animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5mL (every mL containing crude drug 0.36g), total dose is 18g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Continuous Observation 7 days after administration.
Result of the test
In LD50 calculates when giving mice with maximum acceptable concentration and maximum permission capacity (18g/Kg.d), have no dead mouse, namely LD50 is not measured, ask maximum tolerated dose only, in 7 day observation period, its appetite of animal, activity, hair color, the mental status etc. are all normal, grow normal, there are no death.Namely select the 300 times of doses being equivalent to clinical dosage, and have no adverse reaction, show that acute toxicity is minimum, MTD > 18g/Kg.d.
Claims (9)
1. be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, it is characterized in that: comprise with parts by weight: Fructus Aurantii 2 ~ 6 parts, Semen Plantaginis 1 ~ 7 part, Radix Paeoniae Rubra 3 ~ 9 parts, Endothelium Corneum Gigeriae Galli 4 ~ 9 parts, 2 ~ 7 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 1 ~ 9 part, the Rhizoma Anemarrhenae 3 ~ 10 parts, the Radix Astragali 2 ~ 9 parts, Rhizoma Dioscoreae 1 ~ 7 part, 4 ~ 12 parts, the Radix Rehmanniae, Radix Scrophulariae 5 ~ 10 parts, Radix Glehniae 1 ~ 7 part, Radix Ophiopogonis 3 ~ 8 parts, Rhizoma Polygonati Odorati 2 ~ 9 parts, Rhizoma Polygonati 3 ~ 8 parts, Radix Trichosanthis 2 ~ 7 parts, Fructus Corni 4 ~ 10 parts, Fructus Lycii 1 ~ 8 part, Fructus Ligustri Lucidi 2 ~ 9 parts, Herba Epimedii 1 ~ 7 part, Cortex Moutan 3 ~ 10 parts, PORIA from Yunnan of China 3 ~ 8 parts, Radix Aconiti Lateralis Preparata 2 ~ 9 parts, Rhizoma Alismatis 4 ~ 10 parts, Fructus Schisandrae Chinensis 2 ~ 7 parts.
2. the Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease according to claim 1, it is characterized in that: described in be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 3 ~ 5 parts, Semen Plantaginis 2 ~ 6 parts, Radix Paeoniae Rubra 4 ~ 7 parts, Endothelium Corneum Gigeriae Galli 5 ~ 8 parts, 3 ~ 6 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 3 ~ 8 parts, the Rhizoma Anemarrhenae 5 ~ 9 parts, the Radix Astragali 4 ~ 7 parts, Rhizoma Dioscoreae 3 ~ 6 parts, 6 ~ 10 parts, the Radix Rehmanniae, Radix Scrophulariae 6 ~ 9 parts, Radix Glehniae 2 ~ 6 parts, Radix Ophiopogonis 4 ~ 7 parts, Rhizoma Polygonati Odorati 3 ~ 6 parts, Rhizoma Polygonati 4 ~ 7 parts, Radix Trichosanthis 3 ~ 6 parts, Fructus Corni 5 ~ 8 parts, Fructus Lycii 2 ~ 7 parts, Fructus Ligustri Lucidi 4 ~ 8 parts, Herba Epimedii 2 ~ 6 parts, Cortex Moutan 5 ~ 9 parts, PORIA from Yunnan of China 4 ~ 7 parts, Radix Aconiti Lateralis Preparata 3 ~ 8 parts, Rhizoma Alismatis 5 ~ 9 parts, Fructus Schisandrae Chinensis 3 ~ 6 parts.
3. the Chinese medicine composition being used for the treatment of non-alcoholic fatty liver disease according to claim 1, it is characterized in that: described in be used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease, comprise with parts by weight: Fructus Aurantii 4 parts, Semen Plantaginis 3 parts, Radix Paeoniae Rubra 6 parts, Endothelium Corneum Gigeriae Galli 7 parts, 5 parts, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii 5 parts, the Rhizoma Anemarrhenae 8 parts, the Radix Astragali 6 parts, Rhizoma Dioscoreae 4 parts, 9 parts, the Radix Rehmanniae, Radix Scrophulariae 8 parts, Radix Glehniae 5 parts, Radix Ophiopogonis 6 parts, Rhizoma Polygonati Odorati 5 parts, Rhizoma Polygonati 5 parts, Radix Trichosanthis 4 parts, Fructus Corni 7 parts, Fructus Lycii 6 parts, Fructus Ligustri Lucidi 5 parts, Herba Epimedii 4 parts, Cortex Moutan 8 parts, PORIA from Yunnan of China 5 parts, Radix Aconiti Lateralis Preparata 5 parts, Rhizoma Alismatis 8 parts, Fructus Schisandrae Chinensis 5 parts.
4. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 1, is characterized in that: comprise the following steps:
Step 1, get Fructus Aurantii, Semen Plantaginis, Radix Paeoniae Rubra, Endothelium Corneum Gigeriae Galli, Rhizoma Curcumae Longae, Radix Panacis Quinquefolii, the Rhizoma Anemarrhenae, the Radix Astragali, Rhizoma Dioscoreae, the Radix Rehmanniae, Radix Scrophulariae, Radix Glehniae, Radix Ophiopogonis, Rhizoma Polygonati Odorati, Rhizoma Polygonati, Radix Trichosanthis, Fructus Corni, Fructus Lycii, Fructus Ligustri Lucidi, Herba Epimedii, Cortex Moutan, PORIA from Yunnan of China, Radix Aconiti Lateralis Preparata, Rhizoma Alismatis, Fructus Schisandrae Chinensis, after mix homogeneously, be ground into coarse powder, carry out supercritical CO
2extraction, collects extract;
Step 2, adsorbs step 1 gained extract through macroporous adsorbent resin, then carries out eluting with ethanol water, obtain eluent;
Step 3, by step 2 gained eluent after ultrafiltration membrance filter, concentrated, dry, obtain Chinese medicine composition.
5. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 4, is characterized in that: supercritical CO in step 1
2the pressure of extraction is 15 ~ 40MPa, and temperature is 35 ~ 50 DEG C, and extraction time is 2.5 ~ 4h.
6. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 4, is characterized in that: macroporous adsorbent resin described in step 2 is selected from AB-8, NKA, HDP-100, HDP-722 or HDP-750.
7. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 4, is characterized in that: in step 2, the concentration of ethanol water is 70 ~ 80wt.%.
8. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 4, is characterized in that: the molecular cut off of ultrafilter membrane described in step 3 is 100000 ~ 200000Da.
9. the preparation method being used for the treatment of the Chinese medicine composition of non-alcoholic fatty liver disease according to claim 4, is characterized in that: dry in step 3 is lyophilization or spraying dry.
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| RU2644282C2 (en) * | 2016-06-29 | 2018-02-08 | Алексей Алексеевич Тиньков | Means for non-alcoholic fatty liver disease treatment and prevention |
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Application publication date: 20150107 |