CN104257707A - Traditional Chinese medicine extract for treating tympanitis and preparation method of traditional Chinese medicine extract - Google Patents
Traditional Chinese medicine extract for treating tympanitis and preparation method of traditional Chinese medicine extract Download PDFInfo
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Abstract
The invention belongs to the field of traditional Chinese medicines and discloses a traditional Chinese medicine extract for treating tympanitis and a preparation method of the traditional Chinese medicine extract. The traditional Chinese medicine extract is prepared from the following components in parts by weight: 8-15 parts of paniculate microcos leaf alcohol extract, 6-10 parts of radix bupleuri alcohol extract, 3-8 parts of poria cocos ethyl acetate extract and 3-5 parts of negundo chastetree fruit supercritical extract. The preparation method of the traditional Chinese medicine extract comprises the following steps: extracting different traditional Chinese medicines by virtue of different methods such solvent extraction, and supercritical carbon dioxide extraction; mixing the extracts to obtain the traditional Chinese medicine extract for treating tympanitis. The traditional Chinese medicine extract obtained by the preparation method can be used for effectively eliminating or relieving symptoms such as tinnitus, aural fullness, blocking and hearing loss.
Description
Technical field
The invention belongs to tcm field, relate to a kind of Chinese medicine and preparation method thereof, particularly relate to a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.
Background technology
Otitis media mainly with hydrotympanum and auditory dysesthesia for principal character, the initial stage is main mainly with bulge of the ear, and the later stage has the symptoms such as ear pain.In addition because otitis media morbidity is very fast, more than with symptoms such as auditory dysesthesias.TCM investigation is thought, the cause of disease of otitis media mostly is the functional disorders such as liver, spleen, lung, caused by rheumatism cold and heat etc. are piled up in vivo.
Otitis media can be divided into affection due to external wind and heat, affection of exogenous wind-cold etc. according to symptom.The Chinese medicine for the treatment of otitis media is more, the Chinese medicines such as such as Herba Plantaginis, Rhizoma Alismatis, Radix Saposhnikoviae, Fructus Xanthii, Radix Puerariae, Radix Salviae Miltiorrhizae.The patent No. be the disclosure of the invention of 101978968 treatment otitis media Chinese medicine be tarantula and Alumen.What it was too much takes the certain side effect of rear generation.The Western medicine aspect of otitis in the treatment in addition, dexamethasone has good curative effect.Though the Chinese medicine for the treatment of otitis media is many, but its ratio of curing otitis media is completely not high, the clinical research about otitis media still has a lot of problem to need to solve.Need us to probe into mechanism and the pathology of healing otitis media further, filter out more effective Chinese medicine formula.
Summary of the invention
The technical problem solved: use Chinese medicine all poor to the method effect for the treatment of otitis media at present, tinnitus, the ear that can not slow down patient are preferably vexed, blocking, auditory dysesthesia symptom, the problem that the otitis media that result in patient can not effectively be treated, therefore needs a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.
Technical scheme: for the problems referred to above, the invention discloses a kind of Chinese medicine extract for the treatment of otitis media and preparation method thereof.The Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts.
Preferably, described a kind of Chinese medicine extract for the treatment of otitis media, comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts.
Preferred further, described a kind of Chinese medicine extract for the treatment of otitis media, Chinese medicine extract comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts.
Described a kind of preparation method for the treatment of the Chinese medicine extract of otitis media, preparation method comprises the following steps:
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, filter after extracting, filtrate is carried out after vacuum film concentrates, obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, grinding after baking the affected part after applying some drugs is dry, extracts Chinese Thorowax Root dehydrated alcohol after grinding becomes powder, filters after extracting, and prepares Radix Bupleuri ethanol extraction after filtrate is concentrated; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, be separated after extracting, after separation, supernatant concentration obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, collect separating still after extraction, gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
Preferably, described a kind of preparation method for the treatment of the Chinese medicine extract of otitis media, comprise the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, Extracting temperature is 50 DEG C, and extraction time is 2h, and solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
In the Chinese medicine extract that above-mentioned treatment otitis media is large, independent Chinese medicine such as Folium Microcoris paniculatae, Radix Bupleuri, Poria, Fructus Viticis Negundo may not possess the effect for the treatment of otitis media, but after composition effective in above-mentioned four kinds of Chinese medicines is carried out solvent extraction or supercritical carbon dioxide extraction, mixed Chinese medicine extract achieves the good result for the treatment of otitis media.
Beneficial effect: of the present inventionly get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, especially when Folium Microcoris paniculatae ethanol extraction be 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts time, the tinnitus that the treatment otitis media of Chinese medicine extract causes, ear are vexed, blocking, auditory dysesthesia symptom obtain and remove completely or alleviate, effectively can cure otitis media.
Detailed description of the invention
Embodiment 1
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 12 parts, Radix Bupleuri ethanol extraction is 9 parts, Poria ethyl acetate extract is 6 parts, Fructus Viticis Negundo supercritical extract is 4 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 1, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 9 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 1 patient is effectively alleviated.
Embodiment 2
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 8 parts, Radix Bupleuri ethanol extraction is 10 parts, Poria ethyl acetate extract is 8 parts, Fructus Viticis Negundo supercritical extract is 5 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 2, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 6 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 4 patients is effectively alleviated.
Embodiment 3
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) getting that Folium Microcoris paniculatae ethanol extraction is 15 parts, Radix Bupleuri ethanol extraction is 6 parts, Poria ethyl acetate extract is 3 parts, Fructus Viticis Negundo supercritical extract is 3 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 3, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 7 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 3 patients is effectively alleviated.
Embodiment 4
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) getting that Folium Microcoris paniculatae ethanol extraction is 15 parts, Radix Bupleuri ethanol extraction is 6 parts, Poria ethyl acetate extract is 3 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 4, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 3 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 7 patients is effectively alleviated.
Embodiment 5
(1) pulverize after Folium Microcoris paniculatae lyophilization, extract after pulverizing with dehydrated alcohol, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) getting that Folium Microcoris paniculatae ethanol extraction is 8 parts, Radix Bupleuri ethanol extraction is 10 parts, Poria ethyl acetate extract is 8 parts by weight, mixed by said extracted thing, is the Chinese medicine extract for the treatment of otitis media.
10 patients take the Chinese medicine extract of embodiment 5, every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 4 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 6 patients is effectively alleviated.
Contrast experiment
Get that Fructus Forsythiae is 3 parts, Radix Scutellariae is 6 parts, Herba Houttuyniae is 7 parts, Herba Violae is 2 parts, Radix Bupleuri is 8 parts, Radix Glycyrrhizae is 4 parts, decoct relief patient and take.Every day, taking dose was 20g, and once a day, the cycle of taking is 10 days, the tinnitus of 2 patients in 10 patients after 10 days, ear are vexed, blocking, auditory dysesthesia symptom eliminated completely, the symptom of 8 patients is effectively alleviated.
Claims (10)
1. treat a Chinese medicine extract for otitis media, it is characterized in that the Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts.
2. a kind of Chinese medicine extract for the treatment of otitis media according to claim 1, is characterized in that the Chinese medicine extract of described treatment otitis media comprises the extract of following weight parts: Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts.
3. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Folium Microcoris paniculatae ethanol extraction is 12 parts.
4. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Radix Bupleuri ethanol extraction is 9 parts.
5. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Poria ethyl acetate extract is 6 parts.
6. a kind of Chinese medicine extract for the treatment of otitis media according to claim 2, is characterized in that in the Chinese medicine extract of described treatment otitis media, Fructus Viticis Negundo supercritical extract is 4 parts.
7. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 2, it is characterized in that the preparation method of described Chinese medicine extract comprises the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, filter after extracting, filtrate is carried out after vacuum film concentrates, obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, grinding after baking the affected part after applying some drugs is dry, extracts Chinese Thorowax Root dehydrated alcohol after grinding becomes powder, filters after extracting, and prepares Radix Bupleuri ethanol extraction after filtrate is concentrated; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, be separated after extracting, after separation, supernatant concentration obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, collect separating still after extraction, gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
8. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 7, it is characterized in that the preparation method of described Chinese medicine extract comprises the following steps: (1) pulverizes after Folium Microcoris paniculatae lyophilization, extract with dehydrated alcohol after pulverizing, Extracting temperature is 50 DEG C, extraction time is 2h, solid-liquid ratio is 1:6, extract after filter, filtrate is carried out vacuum film be concentrated into without ethanol outflow after obtain Folium Microcoris paniculatae ethanol extraction; (2) Radix Bupleuri is carried out baking the affected part after applying some drugs to do, hot bake out temperature is 85 DEG C, grinding after baking the affected part after applying some drugs is dry, it is extracted by Chinese Thorowax Root dehydrated alcohol after 300 object powder that grinding becomes order number, Extracting temperature is 60 DEG C, and extraction time is 1h, and solid-liquid ratio is 1:5, filter after extracting, after filtrate is concentrated, prepare Radix Bupleuri ethanol extraction; (3) Poria is dried, after drying, Poria is pulverized, extract by ethyl acetate after pulverizing, Extracting temperature is 40 DEG C, and extraction time is 4h, and solid-liquid ratio is 1:4, centrifugalize after extracting, centrifugal rotational speed is 5000rpm, after separation, supernatant concentration is obtained Poria ethyl acetate extract; (4) carry out supercritical carbon dioxide extraction by after Fructus Viticis Negundo vacuum drying, supercritical carbon dioxide extraction pressure is 20MPa, and separating still pressure is 6MPa, extraction temperature is 50 DEG C, extraction time is 2h, collects separating still after extraction, and gleanings is Fructus Viticis Negundo supercritical extract; (5) get that Folium Microcoris paniculatae ethanol extraction is 8 ~ 15 parts, Radix Bupleuri ethanol extraction is 6 ~ 10 parts, Poria ethyl acetate extract is 3 ~ 8 parts, Fructus Viticis Negundo supercritical extract is 3 ~ 5 parts by weight, said extracted thing is mixed, for treating the Chinese medicine extract of otitis media.
9. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 8, is characterized in that described Folium Microcoris paniculatae ethanol extraction is 12 parts.
10. a kind of preparation method for the treatment of the Chinese medicine extract of otitis media according to claim 8, is characterized in that described Fructus Viticis Negundo supercritical extract is 4 parts.
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| CN106260861A (en) * | 2015-06-08 | 2017-01-04 | 杜乃龙 | A kind of diet therapy formula controlling the dark tinnitus of mesh |
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| CN101978968A (en) * | 2010-10-20 | 2011-02-23 | 李西灵 | Medicament for treating tympanitis |
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Application publication date: 20150107 |