CN104255808A - Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control - Google Patents
Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control Download PDFInfo
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- CN104255808A CN104255808A CN201410419402.5A CN201410419402A CN104255808A CN 104255808 A CN104255808 A CN 104255808A CN 201410419402 A CN201410419402 A CN 201410419402A CN 104255808 A CN104255808 A CN 104255808A
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Abstract
The invention discloses a lecanicillium lecanii oil solution, a preparing method thereof and applications of the oil solution in agricultural pest control. A lecanicillium lecanii oil suspension is prepared from following components by weight: 10-30% of lecanicillium lecanii spore powder, 5-20% of an emulsifier, 1-3% of a stabilizing agent and 47-84% of plant oil. The oil suspension is advantageous in that: the spore storage time is long, and after storage at room temperature for 15 months, the rate of live spores is more than 80%; the cost is low, and the amount of oil used for dilution of the oil solution is largely saved; the oil suspension meets conventional pesticide using custom; operation in the field is simple, wherein the oil suspension is directly diluted with water by 1-10 times and then sprayed by an ultra-low volume technology, or is diluted with water by 1000-2000 times and then sprayed in a normal amount; and in a dry environment, the product has good control effects for apple yellow aphids and alfalfa aphid.
Description
Technical field
The invention belongs to microbial pesticide technical field, be specifically related to a kind of Lecanicillium lecanii finish and preparation method thereof and the effect in control pest.
Background technology
Sucking pest (aphid, aleyrodid, thrips etc.) is the important pests of various crop, its prevention and control mainly rely on chemical pesticide, and chemical pesticide a large amount of for a long time use not only heavy damage ecotope, pollute natural environment to cause, and Practice for Pesticide Residue in Agricultural Products exceeds standard also day by day serious.The living standard of people improves constantly in recent years, more and more higher to the requirement of food quality, and pollution-free food and pollution-free food production are arisen at the historic moment.Adopt free from environmental pollution, the biopesticide nontoxic to people and animals substitutes the important means that chemical pesticide becomes crop disease and insect prevention and control.Verticillium lecanii (Lecanicillium Lecanii) is important entomogenous fungi, to the control efficiency that sucking pest has had, this bacterium in West Europe, Russia and the U.S., medium many countries and regions have extensive investigation and application.Fungi mainly relies on biological living (mycelium and spore) to carry out biological control, and its control efficiency, by multiple natural environment, as the impact of the factors such as temperature, humidity, illumination, causes preventive effect unstable.The important content that fungus biocontrol effect becomes fungi preparation research is improved by formulation.Existing Verticillium lecanii formulation has pulvis, finish and wetting powder.Pulvis is the formulation of fungi development and use the earliest, and preparation method is simple, but it is short for storage period to there is spore, in use requires super-humid conditions, Field information less stable.Finish is conducive to extending spore storage period, but must use ultra-low-volume sprayer, is only applicable to do mechanization operation.Wetting powder is easy to use, and prevention and treatment range is wide, but the surfactant added in preparation often affects fungal spore vigor, the same with pulvis, and wetting powder requires high to ambient humidity, and field efficacy is unstable.At the end of the eighties in last century, the appearance of fungal oil suspending agent formulation is a quantum jump of fungal farm chemicals research and apply.Oil-suspending agent successfully overcomes the defect of fungal spore to high humility heavy dependence, and fungal farm chemicals can be used in dry environment.
Summary of the invention
The object of the invention is to solve the deficiencies in the prior art, a kind of Verticillium lecanii oil-suspending agent and the anti-application technology eliminated aphis thereof are provided, this Verticillium lecanii oil-suspending agent spore survival period is long, and dilutable water is sprayed, have that insecticidal activity is high, emulsification completely, good stability etc. a little.
In order to realize above object, the present invention adopts following technical scheme: a kind of Verticillium lecanii oil-suspending agent, count by weight percentage, it is made up of following composition: the vegetable oil of the Verticillium lecanii conidial powder of 10-30%, the emulsifier of 5-20%, the stabilizing agent of 1-3% and 47-84%.
In foregoing invention Verticillium lecanii conidial powder spore content be greater than 50,000,000,000/gram, spore rate of living is greater than 90%, and water content is less than 5%.
In foregoing invention, emulsifier is fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, farm chemical emulgent Gernol, tween or their mixture.
In foregoing invention, stabilizing agent is hydroxylated cellulose, CMC, methylcellulose or their mixture.
In foregoing invention, vegetable oil is soybean oil, rapeseed oil, peanut oil, sunflower oil, corn oil, Rice oil, sesame oil, olive oil, castor oil or their mixture.
The preparation method of above-mentioned Verticillium lecanii oil-suspending agent: above-mentioned Verticillium lecanii conidial powder, emulsifier, stabilizing agent and vegetable oil are added in grinder by described formula ratio, whole process temperature must not higher than 38 DEG C, under 1000-2000 rev/min of speed, grind 5-10 minute, mix.
Verticillium lecanii oil-suspending agent of the present invention is easy to use, to spray after being directly watered dilution during use use, can dilute 1-10 doubly after adopt the spraying of ultra low volume technology or dilute with water 1000-2000 doubly after adopt conventional spray, can be used alone, also can with chemical insecticide conbined usage.
Beneficial effect of the present invention is: (1) adopts vegetable oil to be carrier, and spore storage period extends, and the spore rate alive after 12 months of storing is more than 80%; (2) product contains the emulsifier of high concentration, and field uses direct thin up, removes the oil dilution using price high from, reduces costs; (3) emulsifier and spore compatibility good, and very safe to crops, there is no poisoning; (4) add water when product uses and form the state of oil-in-water, oil parcel spore, sufficient moisture promotes spore germination, and after moisture evaporation, the spore of oil parcel has the characteristic of oil-suspending agent, overcomes the defect of traditional fungi preparation to high humility heavy dependence; (5) control effects of product to apple aphid and clover aphid is good.
Embodiment
Mode below by embodiment further illustrates the present invention, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1: emulsifier is on the impact of Verticillium lecanii conidium vitality in oil-suspending agent
By soybean oil and Verticillium lecanii conidial powder (weight accounting 20%) and emulsifier in grinding machine for grinding 10 minutes (1000 revs/min), whole process temperature must not higher than 38 DEG C, the oil-suspending agent of each configuration 500 grams of different emulsifiers, loads ground conical flask room temperature storage.Dipping with oese after fully being mixed respectively by different oil-suspending agent every month is inoculated on 2% water agar plate (WA) a little, even spread, and 25 ± 1 DEG C of constant temperature culture, microscopy after 16 hours, counts 200-300 spore, calculates germination rate.With the Verticillium lecanii xerospore powder (water content is lower than 5%) 4 DEG C of low temperature seal storages for contrast, each process repetition 5 times.As can be seen from Table 1, Verticillium lecanii spore is stored after 15 months in the oil-suspending agent of high-concentration-emulsifier, its germination rate with contrast indifference, high-concentration-emulsifier in oil-suspending agent to the active unrestraint effect of Verticillium lecanii spore.
Table 1 emulsifier is on the impact of Verticillium lecanii oil-suspending agent miospore vigor
Note: in table, data are the mean value ± standard error (S.E) repeated for 5 times
Embodiment 2: Verticillium lecanii oil-suspending agent is to the control efficiency of apple yellow aphid
By rapeseed oil and Verticillium lecanii conidial powder (weight accounting 20%) and emulsifier in grinding machine for grinding 10 minutes (1000 revs/min), whole process temperature must not higher than 38 DEG C, the oil-suspending agent of each configuration 500 grams of different emulsifiers, loads ground conical flask room temperature storage.Different Verticillium lecanii oil-suspending agent dilute with water 1000 times, conventional dose is 25% deltamethrin milk oil, and blank is clear water.Carry out the investigation of insect population radix before dispenser, investigated rear knapsack hand sprayer and carried out whole strain even spraying to apple tree, spray value is 2250kg/hm
2, within after medicine the 10th day, 20 days and 30 days, carry out preventive effect investigation respectively.Test each process and 3 repetitions are set.As known from Table 1, Verticillium lecanii oil-suspending agent has good control efficiency to apple yellow aphid, and after dispenser, the preventive effect of 10d is all higher than 98%, and after medicine, 30d preventive effect all reaches 100%; Preventive effect after contrast chemicals treatment 10d is significantly lower than oil-suspending agent, and be only 85%, the preventive effect after dispenser 30d is down to 50.9%.
Table 2 Verticillium lecanii oil-suspending agent is to the control efficiency of apple yellow aphid
Note: different Verticillium lecanii oil-suspending agent.A, emulsifier fatty alcohol-polyoxyethylene ether accounting 15%; B, emulsifier alkylphenol polyoxyethylene accounting 15%; C, emulsifier Gernol accounting 20%.In table, data are the mean value ± standard error (S.E) repeated for 5 times
Embodiment 3: Verticillium lecanii oil-suspending agent is to the control efficiency of clover aphid
By soybean salad oil and Verticillium lecanii conidial powder (weight accounting 20%) and emulsifier in grinding machine for grinding 10 minutes (1000 revs/min), whole process temperature must not higher than 38 DEG C, the oil-suspending agent of each configuration 500 grams of different emulsifiers, loads ground conical flask room temperature storage.Different Verticillium lecanii oil-suspending agent dilute with water 1000 times, conventional dose is 40% omethoate emulsifiable solution, and blank is clear water.Alfalfa variety is Canadian ABLE, and clover Aphid Species is mainly pea Acyrthosiphon pisum and clover variegation aphid.Test is arranged on Plant Protection institute, Chinese Academy of Agricultral Sciences of Langfang in Hebei Province experimental plot.At clover aphid occurance peak, with knapsack sprayer, when 7:00-8:00 morning or late 6:00-7:00 time, equably by each process reagent spray in the positive and negative of alfalfa-leaves, spray medicine before investigation insect insect population radix.Take investigation method of fixing a point, each community arranges 5 points, gets 20 branches at random at often, statistics aphid borer population alive.After spray medicine, 1d, 3d, 10d are with the remaining borer population alive of same method investigation.As seen from Table 3, to spray after medicine 1d 3 kinds of Verticillium lecanii oil-suspending agents to the preventive effect of clover aphid all close to or more than 94%, be significantly higher than conventional dose omethoate emulsifiable solution, its preventive effect is 89.8%; After spray medicine 10d, the preventive effect of 3 kinds of Verticillium lecanii oil-suspending agents to clover aphid all reaches 100%, and the preventive effect of conventional dose omethoate emulsifiable solution to clover aphid is reduced to 77.6%.
Table 3 Verticillium lecanii oil-suspending agent is to the control efficiency of clover aphid
Note: different Verticillium lecanii oil-suspending agent.A, emulsifier fatty alcohol-polyoxyethylene ether accounting 15%; B, emulsifier alkylphenol polyoxyethylene accounting 15%; C, emulsifier Gernol accounting 20%.In table, data are the mean value ± standard error (S.E) repeated for 5 times
Embodiment 4: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 285g soybean oil, 100g agricultural emulsifier Gernol, 15g hydroxy cellulose sodium and 100g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 20% (w/w).Dilute with water 10 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 1000 times.
Embodiment 5: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 345g soybean oil, 100g agricultural emulsifier Gernol, 15g hydroxy cellulose sodium and 50g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 10% (w/w).Dilute with water 5 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 500 times.
Embodiment 6: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 310g rapeseed oil, 75g fatty alcohol-polyoxyethylene ether, 15g CMC and 100g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 20% (w/w).Dilute with water 10 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 1000 times.
Embodiment 7: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 360g rapeseed oil, 75g fatty alcohol-polyoxyethylene ether, 15g CMC and 50g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 10% (w/w).Dilute with water 5 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 500 times.
Embodiment 8: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 310g castor oil, 75g alkylphenol polyoxyethylene, 15g methylcellulose and 100g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 20% (w/w).Dilute with water 10 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 1000 times.
Embodiment 9: a kind of Verticillium lecanii oil-suspending agent, it is characterized in that: by 360g castor oil, 75g alkylphenol polyoxyethylene, 15g methylcellulose and 50g Verticillium lecanii conidial powder (50,000,000,000/gram, water content is lower than 5%) add grinding machine for grinding 5 minutes (2000 revs/min), whole process temperature higher than 38 DEG C, must not be mixed with the Verticillium lecanii oil-suspending agent of 10% (w/w).Dilute with water 5 times adopts low capacity technology to spray afterwards or adopts conventional spray after dilute with water 500 times.
Claims (5)
1. a Verticillium lecanii oil-suspending agent, is characterized in that: count by weight percentage, and it is made up of following composition: the vegetable oil of the Verticillium lecanii conidial powder of 10-30%, the emulsifier of 5-20%, the stabilizing agent of 1-3% and 47-84%.
2. Verticillium lecanii oil-suspending agent according to claim 1, is characterized in that: described Verticillium lecanii conidial powder spore content is greater than 50,000,000,000/gram, spore rate of living is greater than 90%, and water content is less than 5%; Described emulsifier is a kind of in fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, farm chemical emulgent Gernol, tween or their mixture; Described stabilizing agent is a kind of in hydroxylated cellulose, CMC, methylcellulose or their mixture; Described vegetable oil is a kind of in soybean oil, rapeseed oil, peanut oil, sunflower oil, corn oil, Rice oil, sesame oil, olive oil, castor oil or their mixture.
3. the preparation method of Verticillium lecanii oil-suspending agent according to claim 1, it is characterized in that: Verticillium lecanii conidial powder, emulsifier, stabilizing agent and the vegetable oil described in claim 2 is added in grinder by described formula ratio, whole process temperature must not higher than 38 DEG C, under 1000-2000 rev/min of speed, grind 5-10 minute, mix.
4. the using method of Verticillium lecanii oil-suspending agent according to claim 1, is characterized in that: described Verticillium lecanii oil-suspending agent dilute with water 1-10 is doubly rear adopts the spraying of ultra low volume technology; Or adopt conventional spray after dilute with water 1000-2000 times.
5. the application of Verticillium lecanii oil-suspending agent according to claim 1, it is characterized in that: described Verticillium lecanii oil-suspending agent has good control efficiency to apple yellow aphid, preventive effect after dispenser after 10 days is higher than 98%, preventive effect after dispenser after 20 days is higher than 99%, and after dispenser, after 30 days, preventive effect reaches 100%; Also have excellent preventive effect to clover aphid, the preventive effect of dispenser after 1 day is higher than 94%, and the preventive effect of dispenser after 3 days is higher than 96%, and the preventive effect of dispenser after 10 days reaches 100%.
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| CN106719623A (en) * | 2016-11-25 | 2017-05-31 | 广东省农业科学院植物保护研究所 | A kind of insecticides for preventing and treating mealybug class pest |
| CN106912501A (en) * | 2017-05-02 | 2017-07-04 | 中国农业科学院植物保护研究所 | The compounded pesticides of Lecanicillium lecanii and rotenone |
| CN110402967A (en) * | 2019-08-19 | 2019-11-05 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae bacterial strain in the medicament of preparation prevention and treatment gray mold |
| CN114097828A (en) * | 2021-11-23 | 2022-03-01 | 安徽农业大学 | Lecanicillium lecanii oil agent and preparation method and application thereof |
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Cited By (4)
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| CN114097828A (en) * | 2021-11-23 | 2022-03-01 | 安徽农业大学 | Lecanicillium lecanii oil agent and preparation method and application thereof |
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Application publication date: 20150107 |