CN104237131B - A kind of flow cell - Google Patents
A kind of flow cell Download PDFInfo
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- CN104237131B CN104237131B CN201410537618.1A CN201410537618A CN104237131B CN 104237131 B CN104237131 B CN 104237131B CN 201410537618 A CN201410537618 A CN 201410537618A CN 104237131 B CN104237131 B CN 104237131B
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- flow cell
- infiltration block
- prism
- chip
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Abstract
The invention discloses a kind of flow cell, including base, it is characterized in that, described base is provided with flow cell main part, described flow cell main part inner transverse is provided with infiltration block, described infiltration block is the tubular that one end is airtight, end cap is passed through airtight in the flow cell main body of corresponding described infiltration block opening end, end cap is provided with at least two opening, the sealed end end face of described infiltration block is provided with aperture, the circulation main component of corresponding described infiltration block sealed end is provided with gasket recess, described gasket recess is embedded in packing ring, working cavity is formed with chip laminating outside described packing ring, described chip is compressed by prism, described prism is compressed by flow cell adnexa.
Description
Technical field
The present invention relates to a kind of surface plasmon resonance imaging technique, particularly relate to a kind of stream of application in this technology
Logical pond.
Background technology
Surface plasma resonance imaging (surface plasmon resonance imaging, SPRi) technology be based on
Surface plasma resonance (surface plasmon resonance, SPR) technology, develops in conjunction with CCD imaging and array chip
A kind of optical image technology got up.The electric charge that surface plasma resonance (SPR) is present on metal and dielectric interface is close
Degree vibration resonance ripple, can be excited by incident electromagnetic wave.When incident illumination travels to optically thinner medium and incident angle from optically denser medium
When suitable scope, in the interface experiences total internal reflection of two kinds of materials.The SPR minor variations pole to interfacial medium refractive index
For sensitivity, when sample and interracial contact, owing to there is absorption or chemical reaction, interface medium refraction index will become
Change.Based on this principle can real-time, the most lossless monitoring biomolecule and between interaction, research biology point
The dynamic process combined between son and dissociate, understands physics and the chemisorbed process at interface.As a kind of strong biology
Molecular analytic tools, surface plasmon resonance biosensor possesses real-time, quick, label-free, favorable reproducibility, highly sensitive, sample without purification
Etc. advantage, it is widely used to protein, nucleic acid, and the detection analysis of little molecule.And surface plasma resonance imaging technology
While possessing surface plasma resonance technology advantage, again by the combination of SPRi technology with array biochip technology,
Different probe arrays is modified on bio-sensing chip surface, it is achieved high-throughout detection and the sieve to testing sample target substances
Choosing.In recent years, for various molecules such as the arrival of adaptation " genome times afterwards comprehensively ", genomics, proteomics and metabolism group
The research that biological group is learned deepens continuously, and surface plasma resonance imaging technology is increasingly by the extensive pass of numerous researchers
Note and paying attention to it is considered to be a kind of very promising high flux bioanalytical method.
The present invention relates in SPRI technology for providing the flow cell of testing sample.
Summary of the invention
In view of the demand of prior art, it is an object of the invention to provide a kind of flow cell in SPRI technology.
A kind of flow cell, including base, it is characterised in that described base is provided with flow cell main part, described flow cell master
Part inner transverse is provided with infiltration block, and described infiltration block is the tubular that one end is airtight, the circulation of corresponding described infiltration block opening end
Passing through end cap on tank main body airtight, end cap is provided with at least two opening, and the sealed end end face of described infiltration block is provided with aperture,
The circulation main component of corresponding described infiltration block sealed end is provided with gasket recess, and described gasket recess is embedded in packing ring, described pad
Forming working cavity with chip laminating outside circle, described chip is compressed by prism, and described prism passes through flow cell adnexa
Compress.
As preferably, flow cell adnexa is correspondingly arranged at the rear of flow cell main part, and described flow cell adnexa includes fastening
Fixing seat on base, is provided with compact heap between described fixing seat and described prism, on described fixing seat, horizontally set can
Screw rod is adjusted to be used for compressing compact heap.
As preferably, the base of flow cell, main part and adnexa are made up of hard aluminium alloy.
As preferably, compact heap side is provided with the groove running through upper and lower surface, and described groove fixes the seamed edge of prism.
As preferably, infiltration block is processed by polymethyl methacrylate.
As preferably, chip through gold-plated process technique, is formed the dot matrix chip of array by microscope slide.
As preferably, packing ring is to be processed by politef.
As preferably, prism is made up of flint glass.
As it has been described above, the flow cell of the present invention is to connect outside by the injection port on end cap and outlet, in conjunction with circulation
The sample detection that the inside in pond completes and the process going out sample waste discharge.Testing liquid passes through injection port, flows through in flowing lumen body
The mainstream channel of the infiltration block hole on infiltration block again, reaches what the flow cell other end was formed by prism, chip and packing ring
Confined space.Liquid flows through chip surface in this space, and the material that the test substance in liquid and chip surface have been modified enters
Row is fully contacted and reacts, it is achieved the process of interaction, then under the promotion of buffer, this part testing liquid is the most subsequently
Flowing to outlet from mainstream channel, be discharged to waste liquid cylinder, whole process just completes once.This flow cell have employed hard aluminium alloy structure
The base become and adnexa, and with the liquid infiltration block with circulation cavity that polymethyl methacrylate processes, formed
The system for the distribution of commodities of precise micro, can detect a small amount of testing sample, and can realize many materials by associative array chip
High throughput analysis.
Accompanying drawing explanation
Fig. 1 is shown as the structural representation I of the flow cell of the embodiment of the present invention.
Fig. 2 is shown as the structural representation II of the flow cell of the embodiment of the present invention.
Fig. 3 is shown as the flow cell side sectional view of the embodiment of the present invention.
Piece mark explanation
1 base,
2 circulation main parts,
3 infiltration blocks,
4 circular end caps,
5 packing rings,
6 chips,
7 prisms,
8 compact heaps,
9 flow cell adnexaes,
10 adjusting screws.
Detailed description of the invention
Below by way of specific instantiation, embodiments of the present invention being described, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also be by the most different concrete realities
The mode of executing is carried out or applies, the every details in this specification can also based on different viewpoints and application, without departing from
Various modification or change is carried out under the spirit of the present invention.
The present embodiment provides a kind of flow cell, and including base 1, base 1 is square stereo block, the side above base 1
Being provided with flow cell main part 2, circulation main part 2 is a upwardly extending stereo block, integrally manufactured with base 1.In flow cell main body 2
Between one circular through hole of horizontally set, dispose infiltration block 3 in through hole.Infiltration block 3 is the cylinder that an one end is airtight, forms stream
Cavity body, the liquid communication volume in flowing lumen body is 30～50ul.Screw thread is passed through on the flow cell of corresponding infiltration block 3 opening
The circular end cap 4 of fixed installation one, is distributed two openings on circular end cap 4, and in the present embodiment, the opening of lower section is injection port,
The opening of top is outlet.All openings are connected with external sample delivery pipeline.The sealed end end face of infiltration block 3 is provided with
Aperture, this aperture testing liquid in flowing lumen body passes.The circulation main body 2 of corresponding infiltration block 3 sealed end is provided with pad
Circle groove, gasket recess is embedded in packing ring 5, the back flat of the packing ring 5 slightly above flow cell main body 2 after embedding.The present embodiment
Flow cell also include chip 6, chip 6 packing washer 5 so that chip 6, packing ring 5 form a reaction chamber.Chip 6 is by one
The individual length of side is that the equilateral prism 7 of 25mm compresses.One vertical plane of prism 7 is close to chip, the seamed edge that binding face is relative
Compressed by compact heap 8.Compact heap 8 is a three-dimensional bulk, and is but vertically arranged a groove, seamed edge and groove fit.
The back side of corresponding flow cell main part 2 is provided with flow cell adnexa 9.Flow cell adnexa 9 is one and is fixed on base
Fixing seat, fixing seat both sides are provided with step, and step is provided with installing hole, install screw by installing hole and are connected with base 1.Gu
It is horizontally arranged with two installing holes in reservation, penetrates the adjusting screw 10 equal with aperture in installing hole, by adjusting screw 10
End props up compact heap 8, compresses prism 7 and chip 6.
The base 1 of this flow cell of the present embodiment, main part 2 and adnexa 9 are made up of hard aluminium alloy.
The infiltration block 3 of the present embodiment is to process with polymethyl methacrylate.The infiltration block 3 using this material is difficult to
Broken, there is the transparency of height, it is easy to observe internal liquid state and whether have bubble to disturb；And there is chemically-resistant examination
Agent and solvent resistance.
The chip 6 of the present embodiment through gold-plated process technique, is formed the dot matrix chip of array by microscope slide.Can be used for height
Flux distribution is applied.Gold-plated process technique is by microscope slide after the cleaning treatment of Piranha solution and acid solution, then spend from
Sub-water is rinsed, and re-uses 70 DEG C of heat treatments with nitrogen one hour after drying up subsequently, finally carries out electron beam evaporation
(electron beam evaporation) processes, and plates the thick Cr of one layer of 2nm, plate one the most again on the microscope slide handled well
The gold that layer 51nm is thick.The dot matrix chip of the present embodiment, it is simply that array chip, i.e. making chip when, according to experiment institute
Need, the when that deposited by electron beam evaporation technology carrying out plated film, optionally carry out the point of the array of 4 × 4,8 × 8,10 × 10 grades,
Each point is the elementary cell of a reaction monitoring, therefore can carry out high-throughout detection.
The packing ring 5 of the present embodiment is to be processed by politef.There is fabulous thermostability, moisture resistance, wear-resistant
Property and corrosion resistance, be suitable for the various conventional buffers of different detection system.Its external diameter 19.05mm, internal diameter 15.88mm.
The prism 7 of the present embodiment be the length of side be the equilateral prism of 25mm, be to be made up of F2 glass material, F2 is one
Plant flint glass, in visible ray and near-infrared spectral range, have good performance, there is high reflectance and low Abbe number, and
Compared with N-SF11, it has higher chemical resistance and slightly higher absorbance.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (8)
1. a flow cell, including base, it is characterised in that described base is provided with flow cell main part, described flow cell main part
Inner transverse is provided with infiltration block, and described infiltration block is the tubular that one end is airtight, the flow cell of corresponding described infiltration block opening end
Passing through end cap in main body airtight, end cap is provided with at least two opening, and the sealed end end face of described infiltration block is provided with aperture, right
The circulation main component answering described infiltration block sealed end is provided with gasket recess, and described gasket recess is embedded in packing ring, described packing ring
Outside forms working cavity with chip laminating, and described chip is compressed by prism, and described prism passes through flow cell adnexa pressure
Tightly.
A kind of flow cell the most according to claim 1, it is characterised in that described flow cell adnexa is correspondingly arranged at flow cell
The rear of main part, described flow cell adnexa includes the fixing seat being fastened on base, between described fixing seat and described prism
Being provided with compact heap, on described fixing seat, horizontally set adjusting screw is used for compressing compact heap.
A kind of flow cell the most according to claim 1 and 2, it is characterised in that the base of described flow cell, main part and adnexa
It is made up of hard aluminium alloy.
A kind of flow cell the most according to claim 2, it is characterised in that described compact heap side is provided with and runs through upper and lower surface
Groove, described groove fixes the seamed edge of prism.
A kind of flow cell the most according to claim 1, it is characterised in that described infiltration block is added by polymethyl methacrylate
Work forms.
A kind of flow cell the most according to claim 1, it is characterised in that described chip by microscope slide through gold-plated science and engineering
Skill, forms the dot matrix chip of array.
A kind of flow cell the most according to claim 1, it is characterised in that described packing ring be processed by politef and
Become.
A kind of flow cell the most according to claim 1, it is characterised in that described prism is made up of flint glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537618.1A CN104237131B (en) | 2014-10-11 | 2014-10-11 | A kind of flow cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537618.1A CN104237131B (en) | 2014-10-11 | 2014-10-11 | A kind of flow cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104237131A CN104237131A (en) | 2014-12-24 |
| CN104237131B true CN104237131B (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410537618.1A Active CN104237131B (en) | 2014-10-11 | 2014-10-11 | A kind of flow cell |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109916815B (en) * | 2019-03-18 | 2021-08-10 | 上海陶术生物科技有限公司 | Drug screening device based on SPR |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6862398B2 (en) * | 2001-03-30 | 2005-03-01 | Texas Instruments Incorporated | System for directed molecular interaction in surface plasmon resonance analysis |
| JP2006242916A (en) * | 2005-03-07 | 2006-09-14 | Fuji Photo Film Co Ltd | Sensor unit using total reflection attenuation, and measuring method |
| CN101131379A (en) * | 2007-08-24 | 2008-02-27 | 浙江大学 | Liquid chromatogram detector based on SPR technology |
| CN101216424A (en) * | 2008-01-09 | 2008-07-09 | 浙江大学 | Full-automatic concentration determination apparatus based on surface plasma resonance technology |
| JP5464398B2 (en) * | 2008-07-17 | 2014-04-09 | 独立行政法人産業技術総合研究所 | Measurement method using flow cell |
| US8330959B2 (en) * | 2009-03-23 | 2012-12-11 | Claypool Christopher L | Multi-channel surface plasmon resonance instrument |
| CN201984033U (en) * | 2010-12-31 | 2011-09-21 | 浙江大学 | SPR (surface plasmon resonance) detector for liquid phase microfluidic analysis system |
| CN102095684B (en) * | 2011-01-13 | 2012-05-23 | 河南农业大学 | Multiple-DOF (degree of freedom) adjusting mechanism of optical surface plasma resonance biosensor |
| JP5177915B2 (en) * | 2011-04-25 | 2013-04-10 | 日本電信電話株式会社 | Flow cell |
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Effective date of registration: 20220727 Address after: 400030 Building 5, Lanyuan, No. 61, Middle University Town Road, Shapingba District, Chongqing Patentee after: International Institute of in vitro diagnostics, Chongqing Medical University Address before: 400016 No. 1, Medical College Road, Yuzhong District, Chongqing Patentee before: Chongqing Medical University |