CN104232657A - Related insect resistant gene OsLRR2 of rice source as well as coding product and application thereof - Google Patents

Abstract

The invention discloses a related insect resistant gene OsLRR2 of a rice source, as well as a coding product and application thereof. The insect resistant gene is in the DNA (deoxyribose nucleic acid) sequence of SEQ ID No.1. The whole coding frame of the gene is the base sequence from the 62nd to the 3358th in SEQ ID No.1, and the gene is used for coding 1098 small molecular weight albumen of amino acid residue. Through the research, the gene is closely related with the insect resistance of rice, so that the resistance of the rice to brown planthoppers can be improved by reducing the expression level of the gene. The insect resistant gene can be widely used for crop breeding, in particular to the insect resistant breeding of the rice.

Description

Paddy rice source anti insect related gene OsLRR2 and coded product thereof and application

Technical field

The present invention relates to biological technical field, particularly relate to a kind of paddy rice source anti insect related gene osLRR2and coded product and application.

Background technology

The world have the population of half nearly with paddy rice ( oryza satival.) be food, as one of most important food crop, but cause a large amount of underproduction of paddy rice because of insect pest every year.Increasingly serious food problem, because of agricultural chemicals spreading unchecked use and cause day by day serious environmental problem etc. to make research find pest-resistant new gene, to cultivate pest-resistant new variety extremely urgent.

Brown paddy plant hopper ( nilaparvata lugens) belong to Hemiptera Hemiptera, Delphacidae Delphacidae, be the important pests on China's rice crop.In recent years, brown paddy plant hopper connect and broke out greatly in year, caused heavy losses to the paddy rice grain yield of China.According to statistics, since the eighties in 20th century, brown paddy plant hopper is approximately 1330-2000 ten thousand hectares at the year occurring area of China, accounts for the half of Monitoring of Paddy Rice Plant Area, and year loss paddy reaches 10-15 hundred million kilograms.2005, brown paddy plant hopper was again in the outburst of south China rice district, and cause paddy rice big area " lice burning ", production loss is very serious.The investigation of the Agriculture of Zhejiang Province Room finds, Zhejiang Province the 5th generation brown paddy plant hopper generating capacity in 2005 every 667 square metres reach more than 300,000 have 66.7 ten thousand hectares, account for 86% of the total cultivated area of paddy rice; Every 667 square metres have 33.3 ten thousand hectares, account for 42% more than 1,000,000; The generating capacity of the highest field reaches every 667 square metre 1000 more than ten thousand; The area of total crop failure reaches 5333 hectares, causes Rice Yield Loss Caused about 1,200,000 tons.

In brown paddy plant hopper Synthetical control system, to cultivate and rice cultivation pest-resistant cultivar prevents and treats one of most economical most effective means of brown paddy plant hopper.But, at present to the gene of Rice Resistance brown paddy plant hopper also clone identification obtain seldom.So far, BPH14 gene is also only had to obtain qualification and clone.Therefore find and identify brown planthopper resistant genes involved in paddy rice, to screening and the cultivation of paddy rice pest-resistant cultivar, realizing the nuisanceless and Sustainable Control fixture of brown paddy plant hopper and be of great significance.

Summary of the invention

The object of the invention is to for the deficiencies in the prior art, a kind of paddy rice source anti insect related gene is provided osLRR2and coded product and application.

The object of the invention is to be achieved through the following technical solutions: a kind of paddy rice source anti insect related gene osLRR2, it has the DNA sequence dna of SEQ ID No.1.

A kind of anti insect related gene osLRR2the albumen of coding, it comprises the protein of the aminoacid sequence of SEQ ID No.2, or the amino acid residue sequence of SEQ ID No.2 is had the protein derivative by SEQ ID No.2 with the identical activity of amino acid residue sequence of SEQ ID No.2 through the replacement of one or more amino-acid residue, disappearance or interpolation.

A kind of paddy rice source anti insect related gene osLRR2application in transgenic plant.

A kind of paddy rice source anti insect related gene osLRR2application in crop breeding.

The invention has the beneficial effects as follows, utilize SSH, RT-PCR and RACE technology separation and cloned osLRR2gene; Utilize quantitative fluorescent PCR (qRT-PCR) methods analyst osLRR2the expression of gene after brown paddy plant hopper is caused harm; Transgenic technology is utilized to obtain osLRR2the RNAi silencing transgene plant of gene, and found osLRR2gene plays very important effect in paddy rice is to Brown Planthopper Resistance.The separating clone of this gene, to the breeding for pest resistance of crop, the especially cultivation of the rice varieties of brown planthopper resistant, has very important guidance and promoter action.

Accompanying drawing explanation

Fig. 1 is osLRR2full-length gene pcr amplification product gel electrophoresis figure; In figure, 1 swimming lane is osLRR2gene PCR electrophoresis result; M is Trans 2K Plus DNA maker;

Fig. 2 is after brown paddy plant hopper is caused harm osLRR2the expression of gene.Con: Culm of Rice is inserted in sky lens.Column diagram is mean number ± standard error (n=5);

Fig. 3 is osLRR2the RNAi silent carrier structural representation of gene;

Fig. 4 is osLRR2gene silencing efficacy figure.In background situation in WT lines (WT), reticent strain L31 and L32 osLRR2the expression amount of gene.In figure, numeral is in reticent strain and WT osLRR2the ratio of gene expression amount.Column diagram is mean number ± standard error (n=5);

Fig. 5 is osLRR2taking food and hobby of laying eggs of brown paddy plant hopper female adult worm is reduced after gene silencing.A (), (b) brown paddy plant hopper bosom ovum female adult worm takes food a number and spawning rate on WT lines (WT), reticent plant L31 and L32." * " represents that treatment group and control group exist significant difference (t-test, p<0.05), " * * " represents that treatment group and control group exist pole significant difference (t-test, p<0.01), and column diagram and linear graph are mean number ± standard error (n=10);

Fig. 6 is osLRR2the survival rate of brown paddy plant hopper nymph is reduced after gene silencing.The survival rate of brown paddy plant hopper nymph on WT lines (WT), reticent strain L31 and L32." * " represents that treatment group and control group exist significant difference (t-test, p<0.05), " * * " represents that treatment group and control group exist pole significant difference (t-test, p<0.01), and linear graph is mean number ± standard error (n=10);

Fig. 7 osLRR2the tolerance of paddy rice to brown paddy plant hopper is added after gene silencing.15 female adult worm are caused harm the resistance to harmful phenotype (n=20) of WT lines (WT) after 18 d, the reticent strain of L31 and L32.

Embodiment

The present invention utilizes SSH, RT-PCR and RACE technology, obtains osLRR2full length sequence.First analyze from SSH clone bank and obtain osLRR2gene fragment, with this fragment design forward and reverse primer, carries out 3 '-RACE and 5 '-RACE respectively and obtains 5 ' end of this gene and 3 ' end group because of PCR fragment, and order-checking splicing acquisition DNA sequence dna.At 5 ' end and 3 ' end non-coding region design primer OsLRR2-F1 and OsLRR2-R1 of splicing DNA sequence dna.Extract paddy rice cane mRNA and reverse transcription becomes cDNA.Take cDNA as template, OsLRR2-F1 and OsLRR2-R1 carry out PCR reaction for primer, namely obtain osLRR2full length sequence sequence verification.Secondly, qRT-PCR is utilized to study osLRR2expression, result show brown paddy plant hopper harm induction of osLRR2expression.Again, Agrobacterium-mediated Transformation method is utilized to obtain osLRR2transgene silencing plant, proved by biological characteristis osLRR2the resistance of paddy rice to brown paddy plant hopper significantly can be increased after gene silencing.The analysis of the separation of this gene and clone and biological function, for the breeding for pest resistance of crop, especially will play important promoter action to the breeding of Rice Resistance brown paddy plant hopper.

Realize concrete technological step of the present invention as follows:

1, paddy rice osLRR2the separation of gene and sequential analysis

First analyze from SSH clone bank and obtain osLRR2gene fragment, with this fragment design forward primer and reverse primer, carries out 3 '-RACE and 5 '-RACE respectively and obtains 5 ' end of this gene and 3 ' end group because of PCR fragment, and order-checking splicing acquisition DNA sequence dna.According to the DNA sequence dna of splicing, we, at its 5 ' end and 3 ' end non-coding region design primer OsLRR2-F1 and OsLRR2-R1, with the cDNA of paddy rice cane mRNA reverse transcription for template, carry out PCR reaction, obtain osLRR2full length gene sequence. osLRR2sequence is shown in SEQ ID No.1.According to the open reading frame (ORF) of this sequence, extrapolate the aminoacid sequence of this gene coded protein, see SEQ ID No.2.

2, brown paddy plant hopper cause harm after osLRR2expression characteristic is analyzed

Paddy rice is caused harm through brown paddy plant hopper after 0,0.5,1,2,4,8,12,24,48 h, gets its cane.Utilizing SV Total RNA Isolation System(Promega) test kit extracts the total RNA of 9 paddy rice canes, and utilizes RNA electrophoresis and spectrophotometer (eppendorf) to detect its concentration and purity.With Reverse Transcription box (PrimeScript RT-PCR Kit, TaKaRa), 0.5 μ g total RNA reverse transcription is become cDNA, concrete operations are with reference to product description.

Quantitative fluorescent PCR (qRT-PCR) detects and uses SsoFast ^tMprobes supermix(Bio-RAD) enzyme premixed liquid preparation reaction system, use CFX96 ^tMreal-Time system(Bio-RAD, California, USA) quantitative PCR apparatus detection fluorescent signal.With paddy rice osACTINgene (TIGR ID:LOC_Os03g50885) is as internal reference, right osLRR2abduction delivering feature carry out analyzing (Fig. 2).

3, osLRR2the acquisition of gene silencing rice strain and the detection to Brown Planthopper Resistance thereof

Build RNAi silent carrier with electric shocking method transformation Agrobacterium, obtain the engineering Agrobacterium containing RNAi carrier.Obtain the callus containing goal gene with the Agrobacterium-mediated Transformation method of this laboratory maturation, then respectively through breaking up in advance, breaking up, take root, obtain osLRR2gene silencing rice plant.The whole growth cycle of transgenic rice lines is observed, does not find physiological defect, can obtain from generation to generation normal.Bioassay test shows: compared with WT lines, osLRR2gene silencing plant reduces taking food of brown paddy plant hopper female adult worm and the survival rate (Fig. 6) of lay eggs hobby (Fig. 5) and brown paddy plant hopper nymph, improves the tolerance (Fig. 7) to brown paddy plant hopper.

Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in the restriction scope of the invention for illustration of the present invention.

Embodiment 1, osLRR2the acquisition of gene and sequential analysis

1) extraction of paddy rice cane RNA, quality examination and total cDNA first chain synthesis

2) with total cDNA first chain for template, carry out PCR reaction, obtain osLRR2gene fragment

OsLRR2-F1：5'- TCCTCCTCAACACCCTTCCT-3'；

OsLRR2-R1：5'- ATGGGCTTTGTTGGTACTGC -3'

Pcr amplification condition: 94 DEG C × 4 min → (94 DEG C × 40 sec → 60 DEG C × 40 sec) × 30 circulation → 72 DEG C × 3.5 min, obtain specific PCR amplification product and see Fig. 1.

3) osHI-LRR2gene base analysis

The PCR primer of acquisition sent Nanjing Jin Sirui company to check order, sequencing result is the sequence SEQ ID No.1 in sequence table.This unnamed gene is by we osLRR2.

After embodiment 2, brown paddy plant hopper cause harm osLRR2expression characteristic is analyzed

1) process of brown paddy plant hopper

Fix a cylinder glass cover (diameter 4 cm, high 8 cm, barrel is uniformly distributed the small ventilating holes that 48 diameters are 0.8 mm) at the cane base portion of paddy rice, access 15 brown paddy plant hopper female adult worm in lens after, top is with foam seal.To cause harm at different time points clip after access brown paddy plant hopper the outer leaf sheath at position, immerse liquid nitrogen immediately ,-80 DEG C save backup.To be inserted in the healthy rice leaf sheath of sky lens in contrast.

2) extraction of RNA and expression characteristic analysis

Utilizing SV Total RNA Isolation System(Promega) test kit extracts the total RNA of paddy rice cane, and utilizes RNA electrophoresis and spectrophotometer (eppendorf) to detect its concentration and purity.With Reverse Transcription box (PrimeScript RT-PCR Kit, TaKaRa), 0.5 μ g total RNA reverse transcription is become cDNA, concrete operations are with reference to product description.

Quantitative fluorescent PCR (qRT-PCR) detects and uses SsoFast ^tMprobes supermix(Bio-RAD) enzyme premixed liquid preparation reaction system, use CFX96 ^tMreal-Time system(Bio-RAD, California, USA) quantitative PCR apparatus detection fluorescent signal.With paddy rice osACTINgene (TIGR ID:LOC_Os03g50885) is as internal reference, right osLRR2abduction delivering feature analyze, concrete reaction system and program are shown in product description, quantification PCR primer and probe as follows:

OsACTIN-P：5’- CGTTTCCGCTGCCCTGAGGTCC -3’

OsACTIN-F：5’- GGACAGGTTATCACCATTGGT -3’

OsACTIN -R：5’- CCGCAGCTTCCATTCCTATG -3’

LRR2-P：5’- CAGAGCTGCACATCAAGCTATGT -3’

LRR2-F：5’- AAGGGAAGAGATGAGAAGTTGAGC -3’

LRR2-R：5’- CTGGGTCATCACCAACATCTCCTTGC -3’

Embodiment 3, osLRR2the acquisition of transgenic strain

1) designing primer will osLRR2after the specific regions of 744 bp of gene expands, utilize DNA subcloning procedures to be connected into pCAMBIA-1301 carrier, obtain the reticent expression vector of RNAi.And by electric shocking method, RNAi carrier is proceeded in Agrobacterium EHA105, for follow-up Plant Transformation.RNAi district primer is as follows:

OsLRR2 -F2：5’-TGAAATAGGCAATCTCGCCATGCTC-3’

OsLRR2–R2：5’- aattctggacaagcaccccactttg -3’

2) Rice Callus is infected with the Agrobacterium containing RNAi carrier.Callus after infecting altogether is placed on screening and culturing about 20 days on the NBDS substratum containing Totomycin, after new kanamycin-resistant callus tissue grows, kanamycin-resistant callus tissue is peeled off from parent, proceed to new screening culture medium NBDS2 subculture and expand numerous.Kanamycin-resistant callus tissue after numerous for expansion is proceeded to division culture medium MS-RG cultivation 2-3 week.Grow after tender shoots until it, excise unnecessary callus and tender shoots is proceeded to MS-RT substratum and take root, differentiate complete T ₀for plant.

3) T ₀for the T that transfer-gen plant obtains ₁for seed, through the Screening of Media containing Totomycin, remove and do not proceed to osLRR2the plant of gene, and single-strain planting, results T ₂for seed.The seed of each individual plant is identified.Obtain and turn osLRR2the homozygous line of gene, with biological function analysis later.

Embodiment 4, osLRR2the pest-resistant functional study of transgenic strain

1) female adult worm feeding selectivity and oviposition selectivity measure: in little plastic cup, put into 1 strain wild-type and 1 plant mutant body paddy rice respectively, seedling spacing 1 cm, 2 strain Culm of Rice base portions fix a cylinder glass cover, access 15 brown paddy plant hopper bosom ovum female adult worm in lens after, top is with foam seal.Observe after connecing worm and record two young plants borer population.After 48 h, remove all brown paddy plant hoppers, add up the egg laying amount on every young plant.This test repetition 10 times.

2) incubate nymph survival rate at the beginning of to measure: seal top at Culm of Rice base portion stationary cylinder shape lens, in lens, at the beginning of access 15 brown paddy plant hoppers, incubate nymph, observe every day and record borer population of surviving.This test repetition 10 times.

3) choose the paddy rice that upgrowth situation is consistent, every strain accesses 15 brown paddy plant hopper female adult worm, and each strain repeats 20 times.Observe the growing state of paddy rice every day and take pictures.Above-mentioned experiment all in greenhouse (26 ± 2 DEG C; Illumination 14 h; Humidity 70%-75%) in carry out.

Embodiment 5, osLRR2the application of gene in paddy rice breeding for pest resistance

(1) what obtain with embodiment 3 turns osLRR2the homozygous line paddy rice of gene is material, analyzes osLRR2the application of gene in paddy rice breeding for pest resistance.

(2) brown paddy plant hopper female adult worm endangers transgenic seedling respectively and contrasts seedling, as shown in Figure 7, within 18 days, contrasts paddy rice afterwards substantially withered, and osLRR2the blade of reticent transgenic paddy rice paddy rice only outside is withered and yellow.Visible osLRR2gene can well be applied to the paddy rice breeding for pest resistance of against-plant hopper.

SEQUENCE LISTING

<110> Zhejiang University

<120> paddy rice source anti insect related gene OsLRR2 and coded product thereof and application

<160> 2

<170> PatentIn version 3.3

<210> 1

<211> 3459

<212> DNA

<213> paddy rice

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ttcctcctca acacccttcc tctgcctgta tctactgctc ctgccgtgtt ttcttctacc 60

gatggcgcat gcggtgcatc gtcatggtgg aatctcactg aggtctcagc agatggccct 120

cctccactgg aaatctactc tccagagcac agggccgcag atgaggagct cttggcaggc 180

cagcacaagc ccctgcaact ggaccggaat cacatgcaga gctgcacatc aagctatgtc 240

ctgggtcatc accaacatct ccttgccaga tgccggcatc catggccagc ttggtgagct 300

caacttctca tctcttccct tcctcacata tattgatctc agcagcaaca gtgtctatgg 360

cccaataccc tcaagtatca gctctctatc agcactcacc taccttgacc ttcaactcaa 420

ccagctcaca ggaagaatgc cggatgagat cagtgagctt cagaggctca ccatgcttga 480

tctttcgtac aacaacctca caggccacat ccctgcatcc gtgggtaacc tgacgatgat 540

caccgagctc tccattcacc gaaatatggt ctctggtccc atcccaaagg agataggaat 600

gcttgccaac ctgcagcttt tgcagctgag caacaacacg ttgagcggcg agataccaac 660

aactctagca aatctgacca acctagatac tttctatcta gatggtaatg aactgtcagg 720

gcctgtacca ccaaaactct gcaagctgac caacttgcaa tatcttgctc ttggtgataa 780

caaacttact ggtgaaatcc ccacatgtat aggcaatctc actaagatga tcaaactcta 840

tctctttaga aaccaaataa ttggttccat tccccctgaa ataggcaatc tcgccatgct 900

caccgatctt gttctcaatg aaaataaatt aaaaggttcc ttacccacag aactaggcaa 960

cctcactatg ctgaacaatc ttttcctcca tgaaaaccaa attactggtt caataccccc 1020

aggactaggg atcatatcaa atctccagaa cttaatcctg cacagcaatc aaatctctgg 1080

ctctatacct ggcaccttag cgaaccttac caagctaatt gcactggacc tcagtaaaaa 1140

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tttggaagag aatcaaattt ccggctcaat accgaaatct ttagggaact ttcaaaacat 1260

gcaaaatctg aattttcgta gtaaccaatt gtcgaattct cttcctcagg aatttggaaa 1320

tatcacaaac atggttgagc tagacctagc aagtaactcc ctttcagggc agttgccagc 1380

aaatatatgt gctggcacaa gcctcaaact tttatttctg tctttaaata tgttcaatgg 1440

ccctgttccg aggagcttga agacgtgcac atcgttggtt cgacttttcc ttgatggcaa 1500

ccagttaacg ggagatatat ccaaacattt tggtgtgtat ccaaaactca aaaagatgag 1560

cttgatgtcc aataggctct ctgggcagat ttcaccaaag tggggtgctt gtccagaatt 1620

agctattcta aatatagcag aaaatatgat cacaggcaca atacctccag ctctgtctaa 1680

attgcccaac ctagttgaac taaaactcag ttctaatcat gtaaatggtg tgattccacc 1740

agaaataggg aatttaataa atctatatag tctgaatttg tcgttcaata agttatcagg 1800

atccatacct tcgcagttgg gaaacctgag agatttagaa taccttgatg tttccagaaa 1860

tagtttgagt ggaccaatac ctgaggaact tgggagatgc accaaactac agttactgag 1920

gatcaacaac aaccacttca gtgggaattt gcctgccaca attggaaatt tagcaagcat 1980

acaaatcatg ctagatgtta gcaataacaa acttgatggt ttgttgccgc aagactttgg 2040

aaggatgcag atgctggtat ttctaaattt atctcacaac cagttcactg gaagaattcc 2100

gacttccttt gcaagcatgg tgagcctatc aacacttgat gcgtcataca ataacttaga 2160

aggaccacta ccagcagggc gactatttca aaatgcttca gcaagctggt ttctcaacaa 2220

taaaggtcta tgcggtaatc tctctggcct gccgtcttgt tactcagcac ctggtcacaa 2280

caaaagaaag ctatttcgtt ttctgttgcc ggttgttctt gttctgggtt ttgccattct 2340

tgctacagtt gttctcggaa cggtgtttat tcataacaag agaaaaccac aagaaagtac 2400

tacagccaaa ggaagggaca tgttctctgt ttggaatttt gatggaagat tagcatttga 2460

ggatattgtc agggcaacag aagatttcga tgacaagtac atcattggag cgggaggtta 2520

tggcaaggtc tatcgagcac aactccagga cgggcaggta gttgctgtga agaagcttca 2580

cacaactgaa gaagggttgg gtgatgaaaa aagattttca tgtgaaatgg aaatcttaac 2640

acaaatccga caacgaagca ttgtcaaact gtatggattc tgctcccatc cagagtacag 2700

gtttcttgtc tatgaataca tagagcaggg aagcctccat atgaccttag ctgatgatga 2760

gttagctaag gcattagatt ggcaaaagag gaatattctt ataaaggatg tagctcaagc 2820

actatgttat ctgcaccacg actgcaatcc accaataatt catcgagata taaccagcaa 2880

caacatctta cttgatacaa ctttgaaggc ttatgtctca gattttggta cagcgaggat 2940

tttgagacct gattcatcta attggagtgc gctagctggt acatatggct acatagctcc 3000

tgagctgtcc tatacatctt tggtgacaga gaaatgtgat gtgtatagtt tcggcatggt 3060

tatgctagag gtagtgattg ggaagcaccc aagagattta ttgcagcatc tgacttcatc 3120

aagggatcac aatataacaa tcaaggaaat cctggattca cgacctttag caccaacaac 3180

gacagaagaa gaaaacatag tttccctcat taaggtggtg ttttcttgct tgaaagcttc 3240

tccgcaagca agaccaacaa tgcaggaggt ctaccagaca ctaattgatt atcagacttc 3300

tagttttcta tccaaaaatt gcagtcgagt tatattggat gagctatggg attcataagc 3360

aactaggtat taaggattac tttcccaatt cattctcata agtagtatca aagttttagt 3420

aaattcaaaa tcatatattg cagtaccaac aaagcccat 3459

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Arg Ser Ile Val Lys Leu Tyr Gly Phe Cys Ser His Pro Glu Tyr Arg

865 870 875 880

Phe Leu Val Tyr Glu Tyr Ile Glu Gln Gly Ser Leu His Met Thr Leu

885 890 895

Ala Asp Asp Glu Leu Ala Lys Ala Leu Asp Trp Gln Lys Arg Asn Ile

900 905 910

Leu Ile Lys Asp Val Ala Gln Ala Leu Cys Tyr Leu His His Asp Cys

915 920 925

Asn Pro Pro Ile Ile His Arg Asp Ile Thr Ser Asn Asn Ile Leu Leu

930 935 940

Asp Thr Thr Leu Lys Ala Tyr Val Ser Asp Phe Gly Thr Ala Arg Ile

945 950 955 960

Leu Arg Pro Asp Ser Ser Asn Trp Ser Ala Leu Ala Gly Thr Tyr Gly

965 970 975

Tyr Ile Ala Pro Glu Leu Ser Tyr Thr Ser Leu Val Thr Glu Lys Cys

980 985 990

Asp Val Tyr Ser Phe Gly Met Val Met Leu Glu Val Val Ile Gly Lys

995 1000 1005

His Pro Arg Asp Leu Leu Gln His Leu Thr Ser Ser Arg Asp His

1010 1015 1020

Asn Ile Thr Ile Lys Glu Ile Leu Asp Ser Arg Pro Leu Ala Pro

1025 1030 1035

Thr Thr Thr Glu Glu Glu Asn Ile Val Ser Leu Ile Lys Val Val

1040 1045 1050

Phe Ser Cys Leu Lys Ala Ser Pro Gln Ala Arg Pro Thr Met Gln

1055 1060 1065

Glu Val Tyr Gln Thr Leu Ile Asp Tyr Gln Thr Ser Ser Phe Leu

1070 1075 1080

Ser Lys Asn Cys Ser Arg Val Ile Leu Asp Glu Leu Trp Asp Ser

1085 1090 1095

Claims (4)

1. a paddy rice source anti insect related gene osLRR2, it is characterized in that, it has the DNA sequence dna of SEQ ID No.1.

2. an anti insect related gene according to claim 1 osLRR2the albumen of coding, it is characterized in that, it comprises the protein of the aminoacid sequence of SEQ ID No.2, or the amino acid residue sequence of SEQ ID No.2 is had the protein derivative by SEQ ID No.2 with the identical activity of amino acid residue sequence of SEQ ID No.2 through the replacement of one or more amino-acid residue, disappearance or interpolation.

3. a paddy rice source according to claim 1 anti insect related gene osLRR2application in transgenic plant.

4. a paddy rice source according to claim 1 anti insect related gene osLRR2application in crop breeding.