CN104232657A - Related insect resistant gene OsLRR2 of rice source as well as coding product and application thereof - Google Patents
Related insect resistant gene OsLRR2 of rice source as well as coding product and application thereof Download PDFInfo
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Abstract
The invention discloses a related insect resistant gene OsLRR2 of a rice source, as well as a coding product and application thereof. The insect resistant gene is in the DNA (deoxyribose nucleic acid) sequence of SEQ ID No.1. The whole coding frame of the gene is the base sequence from the 62nd to the 3358th in SEQ ID No.1, and the gene is used for coding 1098 small molecular weight albumen of amino acid residue. Through the research, the gene is closely related with the insect resistance of rice, so that the resistance of the rice to brown planthoppers can be improved by reducing the expression level of the gene. The insect resistant gene can be widely used for crop breeding, in particular to the insect resistant breeding of the rice.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of paddy rice source anti insect related gene
osLRR2and coded product and application.
Background technology
The world have the population of half nearly with paddy rice (
oryza satival.) be food, as one of most important food crop, but cause a large amount of underproduction of paddy rice because of insect pest every year.Increasingly serious food problem, because of agricultural chemicals spreading unchecked use and cause day by day serious environmental problem etc. to make research find pest-resistant new gene, to cultivate pest-resistant new variety extremely urgent.
Brown paddy plant hopper (
nilaparvata lugens) belong to Hemiptera Hemiptera, Delphacidae Delphacidae, be the important pests on China's rice crop.In recent years, brown paddy plant hopper connect and broke out greatly in year, caused heavy losses to the paddy rice grain yield of China.According to statistics, since the eighties in 20th century, brown paddy plant hopper is approximately 1330-2000 ten thousand hectares at the year occurring area of China, accounts for the half of Monitoring of Paddy Rice Plant Area, and year loss paddy reaches 10-15 hundred million kilograms.2005, brown paddy plant hopper was again in the outburst of south China rice district, and cause paddy rice big area " lice burning ", production loss is very serious.The investigation of the Agriculture of Zhejiang Province Room finds, Zhejiang Province the 5th generation brown paddy plant hopper generating capacity in 2005 every 667 square metres reach more than 300,000 have 66.7 ten thousand hectares, account for 86% of the total cultivated area of paddy rice; Every 667 square metres have 33.3 ten thousand hectares, account for 42% more than 1,000,000; The generating capacity of the highest field reaches every 667 square metre 1000 more than ten thousand; The area of total crop failure reaches 5333 hectares, causes Rice Yield Loss Caused about 1,200,000 tons.
In brown paddy plant hopper Synthetical control system, to cultivate and rice cultivation pest-resistant cultivar prevents and treats one of most economical most effective means of brown paddy plant hopper.But, at present to the gene of Rice Resistance brown paddy plant hopper also clone identification obtain seldom.So far, BPH14 gene is also only had to obtain qualification and clone.Therefore find and identify brown planthopper resistant genes involved in paddy rice, to screening and the cultivation of paddy rice pest-resistant cultivar, realizing the nuisanceless and Sustainable Control fixture of brown paddy plant hopper and be of great significance.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of paddy rice source anti insect related gene is provided
osLRR2and coded product and application.
The object of the invention is to be achieved through the following technical solutions: a kind of paddy rice source anti insect related gene
osLRR2, it has the DNA sequence dna of SEQ ID No.1.
A kind of anti insect related gene
osLRR2the albumen of coding, it comprises the protein of the aminoacid sequence of SEQ ID No.2, or the amino acid residue sequence of SEQ ID No.2 is had the protein derivative by SEQ ID No.2 with the identical activity of amino acid residue sequence of SEQ ID No.2 through the replacement of one or more amino-acid residue, disappearance or interpolation.
A kind of paddy rice source anti insect related gene
osLRR2application in transgenic plant.
A kind of paddy rice source anti insect related gene
osLRR2application in crop breeding.
The invention has the beneficial effects as follows, utilize SSH, RT-PCR and RACE technology separation and cloned
osLRR2gene; Utilize quantitative fluorescent PCR (qRT-PCR) methods analyst
osLRR2the expression of gene after brown paddy plant hopper is caused harm; Transgenic technology is utilized to obtain
osLRR2the RNAi silencing transgene plant of gene, and found
osLRR2gene plays very important effect in paddy rice is to Brown Planthopper Resistance.The separating clone of this gene, to the breeding for pest resistance of crop, the especially cultivation of the rice varieties of brown planthopper resistant, has very important guidance and promoter action.
Accompanying drawing explanation
Fig. 1 is
osLRR2full-length gene pcr amplification product gel electrophoresis figure; In figure, 1 swimming lane is
osLRR2gene PCR electrophoresis result; M is Trans 2K Plus DNA maker;
Fig. 2 is after brown paddy plant hopper is caused harm
osLRR2the expression of gene.Con: Culm of Rice is inserted in sky lens.Column diagram is mean number ± standard error (n=5);
Fig. 3 is
osLRR2the RNAi silent carrier structural representation of gene;
Fig. 4 is
osLRR2gene silencing efficacy figure.In background situation in WT lines (WT), reticent strain L31 and L32
osLRR2the expression amount of gene.In figure, numeral is in reticent strain and WT
osLRR2the ratio of gene expression amount.Column diagram is mean number ± standard error (n=5);
Fig. 5 is
osLRR2taking food and hobby of laying eggs of brown paddy plant hopper female adult worm is reduced after gene silencing.A (), (b) brown paddy plant hopper bosom ovum female adult worm takes food a number and spawning rate on WT lines (WT), reticent plant L31 and L32." * " represents that treatment group and control group exist significant difference (t-test, p<0.05), " * * " represents that treatment group and control group exist pole significant difference (t-test, p<0.01), and column diagram and linear graph are mean number ± standard error (n=10);
Fig. 6 is
osLRR2the survival rate of brown paddy plant hopper nymph is reduced after gene silencing.The survival rate of brown paddy plant hopper nymph on WT lines (WT), reticent strain L31 and L32." * " represents that treatment group and control group exist significant difference (t-test, p<0.05), " * * " represents that treatment group and control group exist pole significant difference (t-test, p<0.01), and linear graph is mean number ± standard error (n=10);
Fig. 7
osLRR2the tolerance of paddy rice to brown paddy plant hopper is added after gene silencing.15 female adult worm are caused harm the resistance to harmful phenotype (n=20) of WT lines (WT) after 18 d, the reticent strain of L31 and L32.
Embodiment
The present invention utilizes SSH, RT-PCR and RACE technology, obtains
osLRR2full length sequence.First analyze from SSH clone bank and obtain
osLRR2gene fragment, with this fragment design forward and reverse primer, carries out 3 '-RACE and 5 '-RACE respectively and obtains 5 ' end of this gene and 3 ' end group because of PCR fragment, and order-checking splicing acquisition DNA sequence dna.At 5 ' end and 3 ' end non-coding region design primer OsLRR2-F1 and OsLRR2-R1 of splicing DNA sequence dna.Extract paddy rice cane mRNA and reverse transcription becomes cDNA.Take cDNA as template, OsLRR2-F1 and OsLRR2-R1 carry out PCR reaction for primer, namely obtain
osLRR2full length sequence sequence verification.Secondly, qRT-PCR is utilized to study
osLRR2expression, result show brown paddy plant hopper harm induction of
osLRR2expression.Again, Agrobacterium-mediated Transformation method is utilized to obtain
osLRR2transgene silencing plant, proved by biological characteristis
osLRR2the resistance of paddy rice to brown paddy plant hopper significantly can be increased after gene silencing.The analysis of the separation of this gene and clone and biological function, for the breeding for pest resistance of crop, especially will play important promoter action to the breeding of Rice Resistance brown paddy plant hopper.
Realize concrete technological step of the present invention as follows:
1, paddy rice
osLRR2the separation of gene and sequential analysis
First analyze from SSH clone bank and obtain
osLRR2gene fragment, with this fragment design forward primer and reverse primer, carries out 3 '-RACE and 5 '-RACE respectively and obtains 5 ' end of this gene and 3 ' end group because of PCR fragment, and order-checking splicing acquisition DNA sequence dna.According to the DNA sequence dna of splicing, we, at its 5 ' end and 3 ' end non-coding region design primer OsLRR2-F1 and OsLRR2-R1, with the cDNA of paddy rice cane mRNA reverse transcription for template, carry out PCR reaction, obtain
osLRR2full length gene sequence.
osLRR2sequence is shown in SEQ ID No.1.According to the open reading frame (ORF) of this sequence, extrapolate the aminoacid sequence of this gene coded protein, see SEQ ID No.2.
2, brown paddy plant hopper cause harm after
osLRR2expression characteristic is analyzed
Paddy rice is caused harm through brown paddy plant hopper after 0,0.5,1,2,4,8,12,24,48 h, gets its cane.Utilizing SV Total RNA Isolation System(Promega) test kit extracts the total RNA of 9 paddy rice canes, and utilizes RNA electrophoresis and spectrophotometer (eppendorf) to detect its concentration and purity.With Reverse Transcription box (PrimeScript RT-PCR Kit, TaKaRa), 0.5 μ g total RNA reverse transcription is become cDNA, concrete operations are with reference to product description.
Quantitative fluorescent PCR (qRT-PCR) detects and uses SsoFast
tMprobes supermix(Bio-RAD) enzyme premixed liquid preparation reaction system, use CFX96
tMreal-Time system(Bio-RAD, California, USA) quantitative PCR apparatus detection fluorescent signal.With paddy rice
osACTINgene (TIGR ID:LOC_Os03g50885) is as internal reference, right
osLRR2abduction delivering feature carry out analyzing (Fig. 2).
3,
osLRR2the acquisition of gene silencing rice strain and the detection to Brown Planthopper Resistance thereof
Build RNAi silent carrier with electric shocking method transformation Agrobacterium, obtain the engineering Agrobacterium containing RNAi carrier.Obtain the callus containing goal gene with the Agrobacterium-mediated Transformation method of this laboratory maturation, then respectively through breaking up in advance, breaking up, take root, obtain
osLRR2gene silencing rice plant.The whole growth cycle of transgenic rice lines is observed, does not find physiological defect, can obtain from generation to generation normal.Bioassay test shows: compared with WT lines,
osLRR2gene silencing plant reduces taking food of brown paddy plant hopper female adult worm and the survival rate (Fig. 6) of lay eggs hobby (Fig. 5) and brown paddy plant hopper nymph, improves the tolerance (Fig. 7) to brown paddy plant hopper.
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in the restriction scope of the invention for illustration of the present invention.
Embodiment 1,
osLRR2the acquisition of gene and sequential analysis
1) extraction of paddy rice cane RNA, quality examination and total cDNA first chain synthesis
2) with total cDNA first chain for template, carry out PCR reaction, obtain
osLRR2gene fragment
OsLRR2-F1:5'- TCCTCCTCAACACCCTTCCT-3';
OsLRR2-R1:5'- ATGGGCTTTGTTGGTACTGC -3'
Pcr amplification condition: 94 DEG C × 4 min → (94 DEG C × 40 sec → 60 DEG C × 40 sec) × 30 circulation → 72 DEG C × 3.5 min, obtain specific PCR amplification product and see Fig. 1.
3)
osHI-LRR2gene base analysis
The PCR primer of acquisition sent Nanjing Jin Sirui company to check order, sequencing result is the sequence SEQ ID No.1 in sequence table.This unnamed gene is by we
osLRR2.
After embodiment 2, brown paddy plant hopper cause harm
osLRR2expression characteristic is analyzed
1) process of brown paddy plant hopper
Fix a cylinder glass cover (diameter 4 cm, high 8 cm, barrel is uniformly distributed the small ventilating holes that 48 diameters are 0.8 mm) at the cane base portion of paddy rice, access 15 brown paddy plant hopper female adult worm in lens after, top is with foam seal.To cause harm at different time points clip after access brown paddy plant hopper the outer leaf sheath at position, immerse liquid nitrogen immediately ,-80 DEG C save backup.To be inserted in the healthy rice leaf sheath of sky lens in contrast.
2) extraction of RNA and expression characteristic analysis
Utilizing SV Total RNA Isolation System(Promega) test kit extracts the total RNA of paddy rice cane, and utilizes RNA electrophoresis and spectrophotometer (eppendorf) to detect its concentration and purity.With Reverse Transcription box (PrimeScript RT-PCR Kit, TaKaRa), 0.5 μ g total RNA reverse transcription is become cDNA, concrete operations are with reference to product description.
Quantitative fluorescent PCR (qRT-PCR) detects and uses SsoFast
tMprobes supermix(Bio-RAD) enzyme premixed liquid preparation reaction system, use CFX96
tMreal-Time system(Bio-RAD, California, USA) quantitative PCR apparatus detection fluorescent signal.With paddy rice
osACTINgene (TIGR ID:LOC_Os03g50885) is as internal reference, right
osLRR2abduction delivering feature analyze, concrete reaction system and program are shown in product description, quantification PCR primer and probe as follows:
OsACTIN-P:5’- CGTTTCCGCTGCCCTGAGGTCC -3’
OsACTIN-F:5’- GGACAGGTTATCACCATTGGT -3’
OsACTIN -R:5’- CCGCAGCTTCCATTCCTATG -3’
LRR2-P:5’- CAGAGCTGCACATCAAGCTATGT -3’
LRR2-F:5’- AAGGGAAGAGATGAGAAGTTGAGC -3’
LRR2-R:5’- CTGGGTCATCACCAACATCTCCTTGC -3’
Embodiment 3,
osLRR2the acquisition of transgenic strain
1) designing primer will
osLRR2after the specific regions of 744 bp of gene expands, utilize DNA subcloning procedures to be connected into pCAMBIA-1301 carrier, obtain the reticent expression vector of RNAi.And by electric shocking method, RNAi carrier is proceeded in Agrobacterium EHA105, for follow-up Plant Transformation.RNAi district primer is as follows:
OsLRR2 -F2:5’-TGAAATAGGCAATCTCGCCATGCTC-3’
OsLRR2–R2:5’- aattctggacaagcaccccactttg -3’
2) Rice Callus is infected with the Agrobacterium containing RNAi carrier.Callus after infecting altogether is placed on screening and culturing about 20 days on the NBDS substratum containing Totomycin, after new kanamycin-resistant callus tissue grows, kanamycin-resistant callus tissue is peeled off from parent, proceed to new screening culture medium NBDS2 subculture and expand numerous.Kanamycin-resistant callus tissue after numerous for expansion is proceeded to division culture medium MS-RG cultivation 2-3 week.Grow after tender shoots until it, excise unnecessary callus and tender shoots is proceeded to MS-RT substratum and take root, differentiate complete T
0for plant.
3) T
0for the T that transfer-gen plant obtains
1for seed, through the Screening of Media containing Totomycin, remove and do not proceed to
osLRR2the plant of gene, and single-strain planting, results T
2for seed.The seed of each individual plant is identified.Obtain and turn
osLRR2the homozygous line of gene, with biological function analysis later.
Embodiment 4,
osLRR2the pest-resistant functional study of transgenic strain
1) female adult worm feeding selectivity and oviposition selectivity measure: in little plastic cup, put into 1 strain wild-type and 1 plant mutant body paddy rice respectively, seedling spacing 1 cm, 2 strain Culm of Rice base portions fix a cylinder glass cover, access 15 brown paddy plant hopper bosom ovum female adult worm in lens after, top is with foam seal.Observe after connecing worm and record two young plants borer population.After 48 h, remove all brown paddy plant hoppers, add up the egg laying amount on every young plant.This test repetition 10 times.
2) incubate nymph survival rate at the beginning of to measure: seal top at Culm of Rice base portion stationary cylinder shape lens, in lens, at the beginning of access 15 brown paddy plant hoppers, incubate nymph, observe every day and record borer population of surviving.This test repetition 10 times.
3) choose the paddy rice that upgrowth situation is consistent, every strain accesses 15 brown paddy plant hopper female adult worm, and each strain repeats 20 times.Observe the growing state of paddy rice every day and take pictures.Above-mentioned experiment all in greenhouse (26 ± 2 DEG C; Illumination 14 h; Humidity 70%-75%) in carry out.
Embodiment 5,
osLRR2the application of gene in paddy rice breeding for pest resistance
(1) what obtain with embodiment 3 turns
osLRR2the homozygous line paddy rice of gene is material, analyzes
osLRR2the application of gene in paddy rice breeding for pest resistance.
(2) brown paddy plant hopper female adult worm endangers transgenic seedling respectively and contrasts seedling, as shown in Figure 7, within 18 days, contrasts paddy rice afterwards substantially withered, and
osLRR2the blade of reticent transgenic paddy rice paddy rice only outside is withered and yellow.Visible
osLRR2gene can well be applied to the paddy rice breeding for pest resistance of against-plant hopper.
SEQUENCE LISTING
<110> Zhejiang University
<120> paddy rice source anti insect related gene OsLRR2 and coded product thereof and application
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 3459
<212> DNA
<213> paddy rice
<400> 1
ttcctcctca acacccttcc tctgcctgta tctactgctc ctgccgtgtt ttcttctacc 60
gatggcgcat gcggtgcatc gtcatggtgg aatctcactg aggtctcagc agatggccct 120
cctccactgg aaatctactc tccagagcac agggccgcag atgaggagct cttggcaggc 180
cagcacaagc ccctgcaact ggaccggaat cacatgcaga gctgcacatc aagctatgtc 240
ctgggtcatc accaacatct ccttgccaga tgccggcatc catggccagc ttggtgagct 300
caacttctca tctcttccct tcctcacata tattgatctc agcagcaaca gtgtctatgg 360
cccaataccc tcaagtatca gctctctatc agcactcacc taccttgacc ttcaactcaa 420
ccagctcaca ggaagaatgc cggatgagat cagtgagctt cagaggctca ccatgcttga 480
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aactctagca aatctgacca acctagatac tttctatcta gatggtaatg aactgtcagg 720
gcctgtacca ccaaaactct gcaagctgac caacttgcaa tatcttgctc ttggtgataa 780
caaacttact ggtgaaatcc ccacatgtat aggcaatctc actaagatga tcaaactcta 840
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tatcacaaac atggttgagc tagacctagc aagtaactcc ctttcagggc agttgccagc 1380
aaatatatgt gctggcacaa gcctcaaact tttatttctg tctttaaata tgttcaatgg 1440
ccctgttccg aggagcttga agacgtgcac atcgttggtt cgacttttcc ttgatggcaa 1500
ccagttaacg ggagatatat ccaaacattt tggtgtgtat ccaaaactca aaaagatgag 1560
cttgatgtcc aataggctct ctgggcagat ttcaccaaag tggggtgctt gtccagaatt 1620
agctattcta aatatagcag aaaatatgat cacaggcaca atacctccag ctctgtctaa 1680
attgcccaac ctagttgaac taaaactcag ttctaatcat gtaaatggtg tgattccacc 1740
agaaataggg aatttaataa atctatatag tctgaatttg tcgttcaata agttatcagg 1800
atccatacct tcgcagttgg gaaacctgag agatttagaa taccttgatg tttccagaaa 1860
tagtttgagt ggaccaatac ctgaggaact tgggagatgc accaaactac agttactgag 1920
gatcaacaac aaccacttca gtgggaattt gcctgccaca attggaaatt tagcaagcat 1980
acaaatcatg ctagatgtta gcaataacaa acttgatggt ttgttgccgc aagactttgg 2040
aaggatgcag atgctggtat ttctaaattt atctcacaac cagttcactg gaagaattcc 2100
gacttccttt gcaagcatgg tgagcctatc aacacttgat gcgtcataca ataacttaga 2160
aggaccacta ccagcagggc gactatttca aaatgcttca gcaagctggt ttctcaacaa 2220
taaaggtcta tgcggtaatc tctctggcct gccgtcttgt tactcagcac ctggtcacaa 2280
caaaagaaag ctatttcgtt ttctgttgcc ggttgttctt gttctgggtt ttgccattct 2340
tgctacagtt gttctcggaa cggtgtttat tcataacaag agaaaaccac aagaaagtac 2400
tacagccaaa ggaagggaca tgttctctgt ttggaatttt gatggaagat tagcatttga 2460
ggatattgtc agggcaacag aagatttcga tgacaagtac atcattggag cgggaggtta 2520
tggcaaggtc tatcgagcac aactccagga cgggcaggta gttgctgtga agaagcttca 2580
cacaactgaa gaagggttgg gtgatgaaaa aagattttca tgtgaaatgg aaatcttaac 2640
acaaatccga caacgaagca ttgtcaaact gtatggattc tgctcccatc cagagtacag 2700
gtttcttgtc tatgaataca tagagcaggg aagcctccat atgaccttag ctgatgatga 2760
gttagctaag gcattagatt ggcaaaagag gaatattctt ataaaggatg tagctcaagc 2820
actatgttat ctgcaccacg actgcaatcc accaataatt catcgagata taaccagcaa 2880
caacatctta cttgatacaa ctttgaaggc ttatgtctca gattttggta cagcgaggat 2940
tttgagacct gattcatcta attggagtgc gctagctggt acatatggct acatagctcc 3000
tgagctgtcc tatacatctt tggtgacaga gaaatgtgat gtgtatagtt tcggcatggt 3060
tatgctagag gtagtgattg ggaagcaccc aagagattta ttgcagcatc tgacttcatc 3120
aagggatcac aatataacaa tcaaggaaat cctggattca cgacctttag caccaacaac 3180
gacagaagaa gaaaacatag tttccctcat taaggtggtg ttttcttgct tgaaagcttc 3240
tccgcaagca agaccaacaa tgcaggaggt ctaccagaca ctaattgatt atcagacttc 3300
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aactaggtat taaggattac tttcccaatt cattctcata agtagtatca aagttttagt 3420
aaattcaaaa tcatatattg cagtaccaac aaagcccat 3459
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Leu Ser Thr Leu Asp Ala Ser Tyr Asn Asn Leu Glu Gly Pro Leu Pro
690 695 700
Ala Gly Arg Leu Phe Gln Asn Ala Ser Ala Ser Trp Phe Leu Asn Asn
705 710 715 720
Lys Gly Leu Cys Gly Asn Leu Ser Gly Leu Pro Ser Cys Tyr Ser Ala
725 730 735
Pro Gly His Asn Lys Arg Lys Leu Phe Arg Phe Leu Leu Pro Val Val
740 745 750
Leu Val Leu Gly Phe Ala Ile Leu Ala Thr Val Val Leu Gly Thr Val
755 760 765
Phe Ile His Asn Lys Arg Lys Pro Gln Glu Ser Thr Thr Ala Lys Gly
770 775 780
Arg Asp Met Phe Ser Val Trp Asn Phe Asp Gly Arg Leu Ala Phe Glu
785 790 795 800
Asp Ile Val Arg Ala Thr Glu Asp Phe Asp Asp Lys Tyr Ile Ile Gly
805 810 815
Ala Gly Gly Tyr Gly Lys Val Tyr Arg Ala Gln Leu Gln Asp Gly Gln
820 825 830
Val Val Ala Val Lys Lys Leu His Thr Thr Glu Glu Gly Leu Gly Asp
835 840 845
Glu Lys Arg Phe Ser Cys Glu Met Glu Ile Leu Thr Gln Ile Arg Gln
850 855 860
Arg Ser Ile Val Lys Leu Tyr Gly Phe Cys Ser His Pro Glu Tyr Arg
865 870 875 880
Phe Leu Val Tyr Glu Tyr Ile Glu Gln Gly Ser Leu His Met Thr Leu
885 890 895
Ala Asp Asp Glu Leu Ala Lys Ala Leu Asp Trp Gln Lys Arg Asn Ile
900 905 910
Leu Ile Lys Asp Val Ala Gln Ala Leu Cys Tyr Leu His His Asp Cys
915 920 925
Asn Pro Pro Ile Ile His Arg Asp Ile Thr Ser Asn Asn Ile Leu Leu
930 935 940
Asp Thr Thr Leu Lys Ala Tyr Val Ser Asp Phe Gly Thr Ala Arg Ile
945 950 955 960
Leu Arg Pro Asp Ser Ser Asn Trp Ser Ala Leu Ala Gly Thr Tyr Gly
965 970 975
Tyr Ile Ala Pro Glu Leu Ser Tyr Thr Ser Leu Val Thr Glu Lys Cys
980 985 990
Asp Val Tyr Ser Phe Gly Met Val Met Leu Glu Val Val Ile Gly Lys
995 1000 1005
His Pro Arg Asp Leu Leu Gln His Leu Thr Ser Ser Arg Asp His
1010 1015 1020
Asn Ile Thr Ile Lys Glu Ile Leu Asp Ser Arg Pro Leu Ala Pro
1025 1030 1035
Thr Thr Thr Glu Glu Glu Asn Ile Val Ser Leu Ile Lys Val Val
1040 1045 1050
Phe Ser Cys Leu Lys Ala Ser Pro Gln Ala Arg Pro Thr Met Gln
1055 1060 1065
Glu Val Tyr Gln Thr Leu Ile Asp Tyr Gln Thr Ser Ser Phe Leu
1070 1075 1080
Ser Lys Asn Cys Ser Arg Val Ile Leu Asp Glu Leu Trp Asp Ser
1085 1090 1095
Claims (4)
1. a paddy rice source anti insect related gene
osLRR2, it is characterized in that, it has the DNA sequence dna of SEQ ID No.1.
2. an anti insect related gene according to claim 1
osLRR2the albumen of coding, it is characterized in that, it comprises the protein of the aminoacid sequence of SEQ ID No.2, or the amino acid residue sequence of SEQ ID No.2 is had the protein derivative by SEQ ID No.2 with the identical activity of amino acid residue sequence of SEQ ID No.2 through the replacement of one or more amino-acid residue, disappearance or interpolation.
3. a paddy rice source according to claim 1 anti insect related gene
osLRR2application in transgenic plant.
4. a paddy rice source according to claim 1 anti insect related gene
osLRR2application in crop breeding.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112126710A (en) * | 2020-11-05 | 2020-12-25 | 浙江大学 | Rice-derived insect-resistant related gene OsLRR6 and encoding product and application thereof |
CN112266919A (en) * | 2020-11-05 | 2021-01-26 | 浙江大学 | Rice source insect-resistant related gene OsIDP1 and encoding product and application thereof |
CN113754783A (en) * | 2021-09-10 | 2021-12-07 | 四川农业大学 | Application of recombinant RLK in plant immune regulation |
CN114276429A (en) * | 2021-12-28 | 2022-04-05 | 中国农业科学院作物科学研究所 | Method for cultivating TaLRK-R gene-transferred wheat with resistance to sheath blight and stem base rot and related biological material thereof |
CN117305266A (en) * | 2023-03-10 | 2023-12-29 | 苏州健雄职业技术学院 | Gene OsBDG1 related to rice stress resistance and application of encoding protein thereof |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112126710A (en) * | 2020-11-05 | 2020-12-25 | 浙江大学 | Rice-derived insect-resistant related gene OsLRR6 and encoding product and application thereof |
CN112266919A (en) * | 2020-11-05 | 2021-01-26 | 浙江大学 | Rice source insect-resistant related gene OsIDP1 and encoding product and application thereof |
CN112126710B (en) * | 2020-11-05 | 2022-08-09 | 浙江大学 | Rice source insect-resistant related gene OsLRR6 and encoding product and application thereof |
CN113754783A (en) * | 2021-09-10 | 2021-12-07 | 四川农业大学 | Application of recombinant RLK in plant immune regulation |
CN113754783B (en) * | 2021-09-10 | 2022-12-13 | 四川农业大学 | Application of recombinant RLK in plant immune regulation |
CN114276429A (en) * | 2021-12-28 | 2022-04-05 | 中国农业科学院作物科学研究所 | Method for cultivating TaLRK-R gene-transferred wheat with resistance to sheath blight and stem base rot and related biological material thereof |
CN114276429B (en) * | 2021-12-28 | 2022-10-11 | 中国农业科学院作物科学研究所 | Method for cultivating TaLRK-R gene-transferred wheat with resistance to sheath blight and stem base rot and related biological material thereof |
CN117305266A (en) * | 2023-03-10 | 2023-12-29 | 苏州健雄职业技术学院 | Gene OsBDG1 related to rice stress resistance and application of encoding protein thereof |
CN117305266B (en) * | 2023-03-10 | 2024-05-03 | 苏州健雄职业技术学院 | Gene OsBDG1 related to rice stress resistance and application of coded protein thereof |
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