CN104212087A - Preparation method of monodisperse fluorescent microspheres - Google Patents
Preparation method of monodisperse fluorescent microspheres Download PDFInfo
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Abstract
The invention discloses a preparation method of monodisperse fluorescent microspheres and relates to the field of high polymer material synthesis. The preparation method is used for solving the technical problems of the existing fluorescent microsphere preparation method that the preparation process is complicated and the addition amount of fluorescent substances is high. The method comprises the following steps: I, adding a styrene monomer into ultrapure water, stirring, adding 1% potassium persulfate, and carrying out condensation reflux to obtain polystyrene microspheres; II, uniformly dispersing a fluorescent indicator in an organic solvent so as to prepare a fluorescent indicator solution; and III, putting the polystyrene microspheres in a brown test sample bottle and adding the fluorescent indicator solution, carrying out ultrasonic dispersion and centrifugalization, and washing with ultrapure water till no fluorescent indicator is left on the surfaces of the microspheres. The method disclosed by the invention is simple and easy to operate and small in use level of the indicator, and the obtained fluorescent microspheres are high in fluorescence intensity, good in light resistance, strong in chemical stability and at a monodisperse state. The particle size of the fluorescent microspheres prepared by the preparation method is 100-400nm. The preparation method is applied to the field of preparation of fluorescent microspheres.
Description
Technical field
The present invention relates to synthesis of polymer material field.
Background technology
Fluorescence refers to when uviolizing to Cucumber, the visible ray of the different colours that these materials can be launched and intensity; And when ultraviolet stops irradiating, the disappearance that the light launched of material is also thereupon very fast, this is just called photoluminescence.
Most of fluorescent indicator belongs to organic dye, polycyclic aromatic hydrocarbons and metallo-organic complex.The fluorescent emission intensity of metallo-organic complex is very large, and optical stability is good, is excellent fluorescent indicator.Porphyrin and metalloporphyrin are the class macrocylc compound that occurring in nature extensively exists, and be a kind of dystectic dark solid, solubization extreme difference, has good chemistry, thermostability.Because porphyrin is a kind of structure of conjugated system, having chromophore, is the desirable compounds as fluorescent substance.
Fluorescent microsphere generally refers to coated at microsphere surface or embed fluorescent substance in microballoon matrix, is accepting to send fluorescence after external energy stimulates.Fluorescent microsphere often with organic or inorganic polymer materials for support substrate, as inorganic siloxanes sol-gel matrix, polystyrene microsphere, polymethylmethacrylate superpolymer etc., what current application was more is phenylethylene micro ball, its preparation method is more, and more ripe method has emulsion polymerization, surfactant-free emulsion polymerization, suspension polymerization and dispersion copolymerization method.Surfactant-free emulsion polymerization is compared with conventional emulsion polymerizations method, and surfactant-free emulsion polymerization has the following advantages: (1) does not use emulsifying agent, reduces production cost, also eliminates the last handling process except emulsifying agent simultaneously, pollute little in some application scenario; (2) the latex particle surface clean obtained, avoids in some application process due to detrimentally affect that the existence of emulsifying agent produces polymeric articles performance; (3) the latex particle monodispersity of gained is good.
Fluorescent substance can be loaded in polystyrene microsphere by swelling absorption method, self-assembly method, entrapping method, chemical bonding, copolymerization method etc.Patent CN1693411 discloses " a kind of fluorescent microsphere and spray drying preparation and application " thereof, but spray-drying process is difficult to operation and easily makes fluorescent indicator lose activity in actual applications.Patent CN102676157 discloses " Fluorescent microsphere marker and preparation method thereof ", it take polystyrene microsphere as carrier, preparation method is simple, but requires that the microsphere surface of synthesis is with active group or chemical bond, and this has had considerable restraint to the carrier of fluorescent substance.
Hydrophobic fluorescence material will be embedded in polystyrene microsphere in experiment is very difficult thing, only having when not modifying phosphor surface increases the fluorescent particles of its consumption guarantee greater amt and is coated in microballoon, the people such as Sang Hyuk Im by PtOEP and SiOEP of water delivery to be embedded in (see Talanta67 (2005) 492 – 497) in polystyrene microsphere up to the ratio of 0.8%.
Patent CN103012812A discloses " a kind of preparation method of water-soluble nano microballoon ", and its method is with segmented copolymer, fluorescent indicator and organic solvent for raw material, and the microsphere fluorescence of preparation is effective.But in reaction, the temperature of reaction that relates to is high, complex steps, should not with operation.
Patent CN1278534 discloses " fluorescently-labeled polymer microsphere and preparation method thereof ", and it adopts the method for physical adsorption by fluorescent indicator load in diffuse-aggregate microballoon, and its operation is numerous and diverse and fluorescent substance add-on is more.
The object of the invention is to provide a kind of prepares uniform particle diameter, reduce trace that fluorescent substance exposes, realizes fluorescent substance adds and it strengthens the short-cut method of its stability.In polymer microballoon, very large application is coated with especially for hydrophobic fluorescence indicator.Adopt swelling absorption method, can conveniently by hydrophobic fluorescent substance load in polystyrene microsphere, indicator add-on is few, can realize its stable existence and fluorescence efficiency is high in aqueous phase.
Summary of the invention
The present invention will solve the existing preparation process complexity preparing the method existence of fluorescent microsphere, the technical problem that fluorescent substance add-on is many, thus the preparation method providing a kind of single dispersing fluorescent microsphere.
The preparation method of a kind of single dispersing fluorescent microsphere of the present invention carries out according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 15 ~ 20min, then the ultrapure water of 100 ~ 200mL is added, mechanical stirring under 300 ~ 400rpm, keep nitrogen atmosphere, add the styrene monomer of 3 ~ 10mL after 20 ~ 30min, add 1% Potassium Persulphate of 8 ~ 10mL after stirring 15 ~ 20min, then setting speed is 400 ~ 500rpm, at temperature is 60 ~ 80 DEG C, condensing reflux reaction 12 ~ 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 6 ~ 8mg
-3~ 1 × 10
-6the fluorescent indicator of mol/L is dispersed in the organic solvent of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10 ~ 15mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 10 ~ 100 μ L, ultrasonic disperse 1 ~ 2h at temperature is 40 ~ 60 DEG C, then centrifugal 10 ~ 15min under the rotating speed of 4000 ~ 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
The present invention includes following beneficial effect:
1, operation is simple in the present invention, and basic organic synthesis instrument just can meet.
2, fluorescent-labeled microspheres of the present invention is easy to synthesis, indicator consumption pettiness, does not have harsh requirement for microsphere surface functional group.
3, the present invention obtains that microsphere fluorescence intensity is high, photostabilization good, and chemical stability is strong, presents monodispersity.
4, the present invention is specially adapted to by direct for hydrophobic fluorescence material load in polymeric matrix, and is disperse phase with water.
5, the particle diameter of single dispersing fluorescent microsphere that prepared by the present invention is 100 ~ 400nm.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the polystyrene microsphere of test three step one preparation;
Fig. 2 is the stereoscan photograph of monodispersed fluorescent microsphere prepared by test three;
Fig. 3 is that the photostabilization of irradiating 0 ~ 50min under 380nm excitation wavelength of monodispersed fluorescent microsphere prepared by test three is investigated; Wherein, be from top to bottom 0 to 50min photostabilization investigate.
Embodiment
Embodiment one: the preparation method of a kind of single dispersing fluorescent microsphere of present embodiment carries out according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 15 ~ 20min, then the ultrapure water of 100 ~ 200mL is added, mechanical stirring under 300 ~ 400rpm, keep nitrogen atmosphere, add the styrene monomer of 3 ~ 10mL after 20 ~ 30min, add 1% Potassium Persulphate of 8 ~ 10mL after stirring 15 ~ 20min, then setting speed is 400 ~ 500rpm, at temperature is 60 ~ 80 DEG C, condensing reflux reaction 12 ~ 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 6 ~ 8mg
-3~ 1 × 10
-6the fluorescent indicator of mol/L is dispersed in the organic solvent of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10 ~ 15mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 10 ~ 100 μ L, ultrasonic disperse 1 ~ 2h at temperature is 40 ~ 60 DEG C, then centrifugal 10 ~ 15min under the rotating speed of 4000 ~ 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
Present embodiment comprises following beneficial effect:
1, operation is simple for present embodiment, and basic organic synthesis instrument just can meet.
2, the fluorescent-labeled microspheres of present embodiment is easy to synthesis, indicator consumption pettiness, does not have harsh requirement for microsphere surface functional group.
3, present embodiment obtains that microsphere fluorescence intensity is high, photostabilization good, and chemical stability is strong, presents monodispersity.
4, present embodiment is specially adapted to by direct for hydrophobic fluorescence material load in polymeric matrix, and is disperse phase with water.
5, the particle diameter of single dispersing fluorescent microsphere that prepared by present embodiment is 100 ~ 400nm.
Embodiment two: present embodiment and embodiment one unlike: in step one under 400rpm mechanical stirring.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: in step one, at temperature is 70 DEG C, condensing reflux reacts 24 hours.Other is identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three unlike: in step 2, fluorescent indicator is eight base porphyrin platinum (PtOEP) or octaethylporphyrin palladiums (PdOEP).Other is identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four unlike: in step 2, organic solvent is ethanol, methyl alcohol, dimethyl formamide, chloroform or tetrahydrofuran (THF).Other is identical with one of embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five unlike: in step 3 at temperature is 40 DEG C ultrasonic disperse 1h.Other is identical with one of embodiment one to five.
Embodiment seven: one of present embodiment and embodiment one to six unlike: in step 3 under the rotating speed of 5000rpm centrifugal 15min.Other is identical with one of embodiment one to six.
By following verification experimental verification beneficial effect of the present invention:
Test one: the preparation method of a kind of single dispersing fluorescent microsphere of this test realizes according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 20min, then the ultrapure water of 200mL is added, mechanical stirring under 400rpm, keep nitrogen atmosphere, add the styrene monomer of 3mL after 30min, add 1% Potassium Persulphate of 8mL after stirring 15min, then setting speed is 400rpm, at temperature is 70 DEG C, condensing reflux reacts 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 7.2mg
-3the PtOEP of mol/L is dispersed in the tetrahydrofuran (THF) of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 100 μ L, ultrasonic disperse 1h at temperature is 40 DEG C, then centrifugal 15min under the rotating speed of 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
The particle diameter of the single dispersing fluorescent microsphere of this test preparation is 100nm.
Test two: the preparation method of a kind of single dispersing fluorescent microsphere of this test realizes according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 20min, then the ultrapure water of 200mL is added, mechanical stirring under 400rpm, keep nitrogen atmosphere, add the styrene monomer of 10mL after 30min, add 1% Potassium Persulphate of 8mL after stirring 15min, then setting speed is 400rpm, at temperature is 70 DEG C, condensing reflux reacts 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 7.2mg
-3the PtOEP of mol/L is dispersed in the tetrahydrofuran (THF) of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 100 μ L, ultrasonic disperse 1h at temperature is 40 DEG C, then centrifugal 15min under the rotating speed of 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
The particle diameter of the single dispersing fluorescent microsphere of this test preparation is 400nm.
Test three: the preparation method of a kind of single dispersing fluorescent microsphere of this test realizes according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 20min, then the ultrapure water of 200mL is added, mechanical stirring under 400rpm, keep nitrogen atmosphere, add the styrene monomer of 5mL after 30min, add 1% Potassium Persulphate of 8mL after stirring 15min, then setting speed is 400rpm, at temperature is 70 DEG C, condensing reflux reacts 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 7.2mg
-3the PtOEP of mol/L is dispersed in the tetrahydrofuran (THF) of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 100 μ L, ultrasonic disperse 1h at temperature is 40 DEG C, then centrifugal 15min under the rotating speed of 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
As shown in Figure 1, as can be seen from Figure 1, the particle diameter of polystyrene microsphere is about 300nm to the stereoscan photograph of polystyrene microsphere prepared by this testing sequence one; As shown in Figure 2, as can be seen from Figure 2, the particle diameter of monodispersed fluorescent microsphere is about 320nm to the stereoscan photograph of monodispersed fluorescent microsphere prepared by this test three; Can be drawn by Fig. 1 and Fig. 2, swelling rear microsphere volume increases, and shows that the solvent being dispersed with fluorescent indicator has been diffused into microballoon inside.
The photostabilization of irradiating 0 ~ 50min under 380nm excitation wavelength of the monodispersed fluorescent microsphere of this test preparation is investigated as shown in Figure 3; Wherein, be that the photostabilization of 0 to 50min is investigated from top to bottom, as can be seen from Figure 3, under irradiating for a long time through 50min, its fluorescence intensity does not vary widely, and decays about 6%.
Test four: the preparation method of a kind of single dispersing fluorescent microsphere of this test realizes according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 20min, then the ultrapure water of 200mL is added, mechanical stirring under 400rpm, keep nitrogen atmosphere, add the styrene monomer of 5mL after 30min, add 1% Potassium Persulphate of 8mL after stirring 15min, then setting speed is 400rpm, at temperature is 70 DEG C, condensing reflux reacts 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 7.2mg
-3the PtOEP of mol/L is dispersed in the tetrahydrofuran (THF) of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 10 μ L, ultrasonic disperse 1h at temperature is 40 DEG C, then centrifugal 15min under the rotating speed of 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
The particle diameter of the single dispersing fluorescent microsphere of this test preparation is 300nm.
Test five: the preparation method of a kind of single dispersing fluorescent microsphere of this test realizes according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 20min, then the ultrapure water of 200mL is added, mechanical stirring under 400rpm, keep nitrogen atmosphere, add the styrene monomer of 5mL after 30min, add 1% Potassium Persulphate of 8mL after stirring 15min, then setting speed is 400rpm, at temperature is 70 DEG C, condensing reflux reacts 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 7.2mg
-3the PtOEP of mol/L is dispersed in the tetrahydrofuran (THF) of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 30 μ L, ultrasonic disperse 1h at temperature is 40 DEG C, then centrifugal 15min under the rotating speed of 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.The particle diameter of the single dispersing fluorescent microsphere of this test preparation is 300nm.
Claims (7)
1. a preparation method for single dispersing fluorescent microsphere, is characterized in that the preparation method of single dispersing fluorescent microsphere carries out according to the following steps:
One, the preparation of polystyrene microsphere: by dry after ultrasonic to glass reactor acetone, methyl alcohol and pure water 15 ~ 20min, then the ultrapure water of 100 ~ 200mL is added, mechanical stirring under 300 ~ 400rpm, keep nitrogen atmosphere, add the styrene monomer of 3 ~ 10mL after 20 ~ 30min, add 1% Potassium Persulphate of 8 ~ 10mL after stirring 15 ~ 20min, then setting speed is 400 ~ 500rpm, at temperature is 60 ~ 80 DEG C, condensing reflux reaction 12 ~ 24 hours, obtains polystyrene microsphere;
Two, the preparation of fluorescent indicator solution: be 1 × 10 by the concentration of 6 ~ 8mg
-3~ 1 × 10
-6the fluorescent indicator of mol/L is dispersed in the organic solvent of 10 ~ 20mL, is mixed with fluorescent indicator solution;
Three, swelling method prepares fluorescent microsphere: the polystyrene microsphere step one of 10 ~ 15mL obtained is in brown test agent bottle, add the fluorescent indicator solution of the step 2 preparation of 10 ~ 100 μ L, ultrasonic disperse 1 ~ 2h at temperature is 40 ~ 60 DEG C, then centrifugal 10 ~ 15min under the rotating speed of 4000 ~ 5000rpm, milli-Q water, to microsphere surface unstressed configuration indicator, obtains monodispersed fluorescent microsphere.
2. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, to is characterized in that in step one mechanical stirring under 400rpm.
3. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, is characterized in that in step one, condensing reflux reacts 24 hours at temperature is 70 DEG C.
4. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, to is characterized in that in step 2 that fluorescent indicator is eight base porphyrin platinum (PtOEP) or octaethylporphyrin palladiums (PdOEP).
5. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, is characterized in that in step 2, organic solvent is ethanol, methyl alcohol, dimethyl formamide, chloroform or tetrahydrofuran (THF).
6. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, to is characterized in that in step 3 ultrasonic disperse 1h at temperature is 40 DEG C.
7. the preparation method of a kind of single dispersing fluorescent microsphere according to claim 1, to is characterized in that in step 3 centrifugal 15min under the rotating speed of 5000rpm.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105717083A (en) * | 2016-02-02 | 2016-06-29 | 内蒙古农业大学 | Intelligent fluorescent label and preparation method and application thereof |
| CN106479483A (en) * | 2016-09-23 | 2017-03-08 | 北京海岸鸿蒙标准物质技术有限责任公司 | A kind of streaming Fluorescent Microsphere Standards matter and preparation method thereof |
| CN111748341A (en) * | 2020-06-30 | 2020-10-09 | 苏州科技大学 | Phenothiazine weak light up-conversion system and preparation method and application thereof |
| CN112342014A (en) * | 2020-10-28 | 2021-02-09 | 安徽为臻生物工程技术有限公司 | Preparation method of monodisperse polymer fluorescent microspheres |
| CN112813699A (en) * | 2020-12-29 | 2021-05-18 | 青岛瑞斯凯尔生物科技有限公司 | Preparation method of fluorescent microspheres |
| CN112851846A (en) * | 2021-01-28 | 2021-05-28 | 浙江大学 | Method for preparing surface carboxylation nano fluorescent microspheres by soap-free emulsion polymerization |
| CN113150000A (en) * | 2021-02-21 | 2021-07-23 | 苏州科技大学 | Multichannel tunable weak light up-conversion light-emitting system and application thereof |
| CN113667048A (en) * | 2021-08-16 | 2021-11-19 | 安徽为臻生物工程技术有限公司 | Monodisperse polymer color microsphere and preparation method and application thereof |
| CN115573167A (en) * | 2022-08-11 | 2023-01-06 | 武汉纺织大学 | Water-based fluorescent microsphere, water-based fluorescent microsphere textile, and preparation method and application thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333675A (en) * | 2013-03-18 | 2013-10-02 | 杭州德安奇生物工程有限公司 | Fluorescent microsphere suitable for rapid immunology detection and preparation method thereof |
-
2014
- 2014-09-19 CN CN201410482293.1A patent/CN104212087B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333675A (en) * | 2013-03-18 | 2013-10-02 | 杭州德安奇生物工程有限公司 | Fluorescent microsphere suitable for rapid immunology detection and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| LI SONG ET AL: "Facile process for preparation of PS/PtOEP composite microspheres and their oxygen sensing properties", 《ADVANCED MATERIALS RESEARCH》 * |
| 彭超等: "溶胀法制备含铕配合物的聚苯乙烯荧光微球", 《化工新型材料》 * |
| 聂微: "聚苯乙烯荧光微球的制备及性能考察", 《沈阳药科大学学报》 * |
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| CN105717083A (en) * | 2016-02-02 | 2016-06-29 | 内蒙古农业大学 | Intelligent fluorescent label and preparation method and application thereof |
| CN106479483A (en) * | 2016-09-23 | 2017-03-08 | 北京海岸鸿蒙标准物质技术有限责任公司 | A kind of streaming Fluorescent Microsphere Standards matter and preparation method thereof |
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| CN111748341A (en) * | 2020-06-30 | 2020-10-09 | 苏州科技大学 | Phenothiazine weak light up-conversion system and preparation method and application thereof |
| CN112342014A (en) * | 2020-10-28 | 2021-02-09 | 安徽为臻生物工程技术有限公司 | Preparation method of monodisperse polymer fluorescent microspheres |
| CN112813699A (en) * | 2020-12-29 | 2021-05-18 | 青岛瑞斯凯尔生物科技有限公司 | Preparation method of fluorescent microspheres |
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| CN112851846B (en) * | 2021-01-28 | 2022-04-01 | 浙江大学 | Method for preparing surface carboxylation nano fluorescent microspheres by soap-free emulsion polymerization |
| CN113150000A (en) * | 2021-02-21 | 2021-07-23 | 苏州科技大学 | Multichannel tunable weak light up-conversion light-emitting system and application thereof |
| CN113150000B (en) * | 2021-02-21 | 2022-04-15 | 苏州科技大学 | Multichannel tunable weak light up-conversion light-emitting system and application thereof |
| WO2022174494A1 (en) * | 2021-02-21 | 2022-08-25 | 苏州科技大学 | Multi-channel tunable weak light up-conversion luminescence system and application thereof |
| CN113667048A (en) * | 2021-08-16 | 2021-11-19 | 安徽为臻生物工程技术有限公司 | Monodisperse polymer color microsphere and preparation method and application thereof |
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