CN104206178B - A kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient - Google Patents
A kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient Download PDFInfo
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- CN104206178B CN104206178B CN201410502242.0A CN201410502242A CN104206178B CN 104206178 B CN104206178 B CN 104206178B CN 201410502242 A CN201410502242 A CN 201410502242A CN 104206178 B CN104206178 B CN 104206178B
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Abstract
The present invention discloses a kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient, the present invention is by adopting zine ion to coerce Cordyceps militaris (L.) Link., in conjunction with substratum adds anistree composition, gluconic acid zinc solution is sprayed in the particular growth stage of Cordyceps sporophore, can bringing and strong coerce effect, main active ingredient greatly improves.
Description
Technical field
The present invention relates to Cordyceps militaris (L.) Link. artificial cultivation technique field, particularly relate to a kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient.
Background technology
(Cordycepsmi1itaris, has another name called Cordyceps militaris (L.) Link. to Cordyceps militaris (L.) Link., belongs to Ascomycotina on fungal taxonomy, gang pyrenomycetes, ergot order, Clavicipitaceae, Cordyceps, is all the one of Cordyceps sinensis fungus with Cordyceps sinensis (Cordycepssinensis). Cordyceps sinensis fungus is the general designation of the parasitic class entomogenous fungi mixture formed in the underground sporophore of insect, spider or big group capsule platymiscium. Cordyceps militaris (L.) Link. is the type species of Cordyceps sinensis fungus, and main host plants is on the larva and pupa of some insects of lepidopteran, Coleoptera and Diptera in the wild, and it is " taste is sweet, property is flat; useful lung kidney; mend marrow, hemostasis and phlegm " that the classic of TCM records its effect, basically identical with the function of Cordyceps sinensis. Cordyceps militaris (L.) Link. is the medicine-food two-purpose fungi generally acknowledged both at home and abroad, and Cordyceps militaris (L.) Link. contains cordycepin, gland former times, polysaccharide, lectin isoreactivity material, has anti-swollen cancer, anti-inflammation, antiviral, the effects such as enhancing body immunizing power. Among the people for pneumonia, suffer from a deficiency of the kidney, the treatment of the disease such as pain in the back, its in reducing blood-fat, prevent and treat arteriosclerosis, protection heart and brain tissues, tranquilizing soporific, enhancing macrophage activity, the aspects such as inflammation anticancer, anti-, antibacterial, anti-hypoxia have good effects.
Cordycepin is one of effective ingredient main in Cordyceps militaris (L.) Link., and cordycepin (cordycepin) has another name called 3 �� mono-Desoxyadenosines, is that Cuningham is separated a kind of adenosine class active substance obtained in nineteen fifty-one, and molecular formula is C10H13N503, molecular weight is 251.24, fusing point 230-231 DEG C. Cordycepin is one of effective ingredient main in Cordyceps militaris (L.) Link., has anti-bacteria and anti-virus, antitumor and antifatigue, inflammation raising human immunological competence anti-ageing, anti-, suppresses activity of monoamine oxidase, have higher pharmaceutical use and pharmacological action widely. Clinical main application for the treatment of cancer, leukemia, expansion bronchus, significantly strengthen feritin, the anti-ageing effect of waiting for a long time.
In the past, due to Cordyceps militaris (L.) Link., output is rare in the wild, and its exploitation are not valued by the people. But from late nineteen nineties in last century, since its extensive artificial culture succeeds, its value of exploiting and utilizing is just more and more recognized, particularly for cordycepin research get more and more. Adopt the technological method of artificial culture Cordyceps militaris (L.) Link. mycelium, the Chinese caterpillar fungus culture higher than Natural C.militaris pharmaceutical use can be obtained within the short period of time, greatly reduce the cost that people take Cordyceps militaris (L.) Link., be the effective means effectively solving Chinese caterpillar fungus imbalance between supply and demand.
Summary of the invention
It is an object of the invention to provide a kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient, it is possible to effectively improve the content of the main active ingredient in Cordyceps militaris (L.) Link..
The present invention is achieved by the following technical solution:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 13.85%, star aniseed powder 1.0%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculate the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, when substratum top layer forms small sporophore, again spray the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1% on substratum top layer, fountain height is as the criterion just to be covered by substratum, cultivates after 10-12 days, take out culture, crushed after being dried.
Anise of the present invention (formal name used at school: Illiciumverum), also known as star anise, aniseed and star anise, it it is a kind of plant of star anise order Winteraceae Illicium, its dry fruit is one of food flavouring of Chinese dishes and south east asia culinary art, and star aniseed powder refers to pulverize dried anise and obtains. Anistree fruit contains containing volatile oil, fatty oil, protein, resin etc., and extract is olium anisi, and wherein containing olium anisi 1.7-2.7% in seed, the star anise oil content of dry fruit and dry leaf is respectively 12-13%, 1.6-1.8%. The main component of olium anisi is phenylmethylether (Anethole), aubepine (Anisaldehyde) and fennel ketone (Anisylacetone), safrole (Safrole), phellandrene etc. Olium anisi energy stimulating gastrointestinal neural blood vessel, promoting digestion liquid is secreted, and increases gastrointestinal peristalsis, has effect of stomach invigorating, promoting the circulation of qi, contributes to alleviating spasm, alleviating pain; Phellandrene can promote that medullary cell is ripe and is released into peripheral blood, has the effect of obvious leukocyte increasing, mainly raises neutrophil leucocyte, can be used for leukopenia.
Compared with prior art, tool of the present invention has the following advantages:
1, present invention applicant finds through research, adds anistree in the substratum of Cordyceps militaris (L.) Link., it is possible to utilize the hormesis of anistree composition well, the raising of induction Cordyceps militaris (L.) Link. main active ingredient content.
2, zine ion is had extremely strong accumulation ability by Cordyceps mycelium, under zine ion is coerced, in mycelium, cordycepin content can increase, prior art, to the application of zine ion, generally adopts and adds zine ion in culture medium prescription, and the benefit done like this is easy to operate, weak point is that mycelium just contacts zine ion in the starting stage, slowly adapting to, by the time need the stage stimulating cordycepin to increase, the effect that zine ion is coerced is not good. The cordycepin content not adding the sporophore obtained that zine ion is coerced is 0.05-0.10%; Adopting and add zine ion in the medium, sporophore cordycepin content can reach 0.15-0.25%; The present invention by spraying zine ion solution on specific stage substratum top layer, it is possible to bringing and strong coerce effect, sporophore cordycepin content can reach 0.25-0.35%.
3, prior art, employing zine ion is coerced, the concentration of zine ion solution is higher, such as adopt zinc acetate, zinc acetate accounts for the 2-9% of substratum mass ratio, and the strength of solution of the Zinc Gluconate of the sprinkling of the employing of the present invention is 0.05-0.10%, and consumption is few, cost-saving, and Zinc Gluconate can provide fermentation need glucose.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1-5:
In order to verify the technique effect of the present invention, 2014, it may also be useful to the technology of the present invention produces the product of 5 batches, the method for each batch was as follows:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 13.85%, star aniseed powder 1.0%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculate the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, when substratum top layer forms small sporophore, again spray the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1% on substratum top layer, fountain height is as the criterion just to be covered by substratum, cultivates after 10-12 days, take out culture, crushed after being dried.
Comparative example 1:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 14.85%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculating the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, cultivates after 10-12 days, takes out culture, crushed after being dried.
Comparative example 2:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 13.85%, star aniseed powder 1.0%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculating the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, cultivates after 10-12 days, takes out culture, crushed after being dried.
Comparative example 3:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 14.85%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculate the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, when substratum top layer forms small sporophore, again spray the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1% on substratum top layer, fountain height is as the criterion just to be covered by substratum, cultivates after 10-12 days, take out culture, crushed after being dried.
Result:
Each batch of stochastic sampling, after testing, the results are shown in following table:
Claims (1)
1. one kind is improved the cultural method of Cordyceps militaris (L.) Link. main active ingredient, it is characterised in that:
Comprise the steps:
1), the preparation of bacterial classification: the Cordyceps militaris spawn preserved on PDA inclined-plane nutrient agar is inoculated into liquid PDA substratum, culture temperature 25 DEG C, shaking speed 120r/min, activation culture 4 days, stand-by;
2), the preparation of fermention medium: according to following formulated fermention medium, composition is calculated by weight to: thick wheat bran 30%, maple wood chip 30%, Semen Maydis powder 25%, analysis for soybean powder 13.85%, star aniseed powder 1.0%, MgS040.10%, KH2P040.05%, material-water ratio is 1:1.2, and adjust pH is 5.8,121 DEG C of autoclaving 60min;
3), sporophore is cultivated: after substratum cools, inoculate the Cordyceps militaris spawn activated, Cordyceps militaris spawn inoculum size is the 5-15% of substratum gross weight, light culture, culture temperature 25 DEG C, when substratum top layer forms small sporophore, spray the gluconic acid zinc solution that one layer of mass concentration is 0.05-0.1% again on substratum top layer, fountain height is as the criterion just to be covered by substratum, cultivates after 10-12 days, take out culture, crushed after being dried.
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| CN201410502242.0A CN104206178B (en) | 2014-09-26 | 2014-09-26 | A kind of cultural method improving Cordyceps militaris (L.) Link. main active ingredient |
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| CN104206178B true CN104206178B (en) | 2016-06-01 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101748072A (en) * | 2008-11-28 | 2010-06-23 | 江苏江南生物科技有限公司 | Cordyceps militaris culture medium |
| KR101165041B1 (en) * | 2010-03-09 | 2012-07-20 | 대한민국 | Culture method for fruiting body of cordyceps militaris using living silkwowm pupae |
| KR101347219B1 (en) * | 2011-11-24 | 2014-01-03 | 한라산동충하초영농조합법인 | a Method of Cultivation Cordyceps Militaris |
| CN104058862B (en) * | 2014-06-30 | 2016-03-30 | 桂林丰茂源农业技术开发有限公司 | A kind of glossy ganoderma substituting stuff cultivation substratum that with the addition of anise |
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