CN104206170A - Cultivation method for DNJ enriched lucid ganoderma - Google Patents
Cultivation method for DNJ enriched lucid ganoderma Download PDFInfo
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Abstract
The invention discloses a cultivation method for DNJ enriched lucid ganoderma. Mulberry branches, mulberry roots, mulberry leaf powder, silkworm excrement and the like, which are rich in DNJ, are taken as main cultivating materials, and by improving the cultivation method and using biotransformation, DNJ peculiar to mulberries are gathered into lucid ganoderma sporocarp. Lucid ganoderma cultivated by using the cultivation method has a sufficient source of raw materials, the technique is simple and easy, the cost is low, and the efficiency is high; on the basis of maintaining original medicinal components of the lucid ganoderma, the content of DNJ in sporocarp can reach more than 0.6 percent, which is far higher than the content of DNJ in all organs of mulberries. The lucid ganoderma prepared by the cultivation method can control blood sugar, improve the body quality of diabetic patients, and is of great significance in reducing and replacing the use of insulin, a material on aspects of developing blood sugar reducing health-care products having no side effects is provided, moreover, a rich raw material can be provided for pharmaceutical enterprises extracting medicinal component of DNJ.
Description
Technical field
The present invention relates to the cultural method of a kind of glossy ganoderma, specifically the cultural method of a kind of glossy ganoderma of enrichment DNJ composition.
Background technology
DNJ is the abbreviation of 1-DNJ, is a kind of natural alkaloid of finding from mulberry tree, is a kind of potent glycometabolism enzyme inhibitor, has obvious blood sugar reducing function, is called as glucokinin; DNJ in mulberry tree is mainly distributed in leaf, root and the branch of mulberry tree; Record according to pertinent literature, in mulberry leaf, Sang Gen and mulberry branch dry matter, DNJ content is respectively 0.11%, 0.14% and 0.176%, in this external silkworm excrement, also contains DNJ composition in 1.13% left and right.Glossy ganoderma is a kind of famous and precious traditional Chinese medicine, contains the multiple nutritional and officinal compositions useful to human body, but does culture base-material and use conventional methods the glossy ganoderma of cultivation with common weedtree, almost can't detect the content of DNJ, and blood sugar reducing function is not obvious; This invention is to utilize mulberry branch, Sang Gen, mulberry leaf powder and silkworm excrement etc. to do main culture base-material, utilize biological transformation, distinctive mulberry tree DNJ composition is enriched in ganoderma lucidum fruitbody, the glossy ganoderma that profit is produced in this way, retaining on the basis of original active ingredient, carry out DNJ mensuration by high performance liquid chromatograph, its content is up to 0.6459%.
Summary of the invention
The present invention is to provide " cultural method of a kind of glossy ganoderma of enrichment DNJ composition "
The invention discloses the cultural method of a kind of glossy ganoderma of enrichment DNJ composition, to utilize mulberry branch, Sang Gen, mulberry leaf powder and the silkworm excrement etc. that are rich in DNJ composition to do main culture base-material, by improving cultural method, utilize biological transformation, distinctive mulberry tree DNJ composition is enriched in ganoderma lucidum fruitbody, and the glossy ganoderma that profit is produced in this way, is retaining on the basis of original active ingredient, carry out DNJ mensuration by high performance liquid chromatograph, its content is up to 0.6459%.
In order to achieve the above object, the technical scheme that the present invention takes is:
A cultural method for the glossy ganoderma of enrichment DNJ composition, is characterized in that, comprises the following steps:
Its culture matrix is made up of the raw material of following weight portion: ramulus mori bits 65-75, mulberry root bits 10-20,, silkworm excrement 1-10, mulberry leaf powder 1-10, wheat bran 1-10, Cottonseed Meal 1-5, sucrose 1-5, land plaster 1-5, pulverized limestone 0.1-1, calcium carbonate 0.1-1;
(1) pulverizing of getting the raw materials ready: choosing is dry for subsequent use after crushed without the mulberry branch, Mulberry Roots, mulberry leaf, the silkworm excrement that go mouldy, that other auxiliary material requires is fresh, without going mouldy;
(2) major ingredient is prewetted: ramulus mori bits, mulberry root bits to bacterium rod use processed mix in advance in proportion, add water and prewet;
(3) spice pack: the ramulus mori bits after prewetting, mulberry root bits fully mix by formula rate with silkworm excrement, mulberry leaf powder, wheat bran, Cottonseed Meal, land plaster, pulverized limestone, calcium carbonate, sucrose is put into container and is dissolved in water, then add and stir gradually, then add water, limit edged stirs, and water content requires to reach 55% left and right; Pack the culture base-material of mixing into bacterium bag, tighten sack, make bacterium rod;
(4) sterilizing: by the bacterium rod making sterilizing 12-15 hour under 100 DEG C of normal pressures, sterilizing finishes rear equitemperature and naturally drops to 60 DEG C of right and lefts and can take the dish out of the pot, and natural temperature is down in the cooling place that charge bar is moved into after cleaning, sterilization;
(5) inoculation: before inoculation,, to the strict cleaning of transfer room process, sterilization, inoculating hood and inoculating appliance thoroughly clean, inoculating hood and inoculating appliance sterilization select aerial fog disinfectant 4～8g fumigation about 30 minutes; Inoculating appliance, the outer list processing of bacterium rod and inoculator's both hands should adopt 75% alcohol or 0.2% liquor potassic permanganate scouring sterilization; After sterilization finishes, just can play cave inoculation to bacterium rod, carry out bagging sealing outward connecting the bacterium rod of planting in time; After inoculation, clear up in time residue in inoculating hood, carry out ventilation;
(6) Mycelium culture management: stack connecting groined type in the excellent baterial cultivation chamber that moves into strict desinsection, sterilization of the bacterium of planting, pile high 6-8 layer, carry out Mycelium culture; After inoculation, 8-10 days temperature are controlled at 25 DEG C of left and right, and after 18-20 days, room temperature is controlled at 23 DEG C of left and right, running check to note heap temperature, heap temperature rise, strengthen ventilation, in the time that white hypha body grows into diameter 12cm-15cm around inoculation hole, remove outer bagging with ventilate, promote growth; After 35-40 days, room temperature is controlled at 20-22 DEG C, and mycelium can, at bacterium rod two ends clip, promote fruit body development when covering with and seeing adularescent projection sesame flower bud after bag, then just can move into out Zhi Peng, carries out grand style;
(7) management in fruit body stage: selecting has water source, high-land, free of contamination place to build sesame canopy, and after strict desinsection, sterilization, bacterium rod just can move into out Zhi Peng and carry out grand style; When grand style, bacterium rod is lain in to ground, bacterium interrod spacing 3-5cm, fill up the vegetable garden soil that is rich in humus, above cover the thin sand and soil of 1-2cm, each 1-2 hour sooner or later every day, utilize micro-nozzle intermittent spray shape water, keep that relative moisture is in 85-95% left and right in canopy, about 7 days, just visible ganoderma lucidum fruitbody young sesame flower bud grows, and cuts off too much Zhi Lei with the scissors of sterilizing, every rod retains 2-4 of sesame flower bud, and ganoderma lucidum fruitbody maturation just can be gathered drying afterwards; Gather after the 1st tide, the 5-7 days that cuts off the water, after the wound healing causing because of harvesting fruit body, and then is interrupted water spray, keeps relative moisture more than 85%, enters the management of the growth and development stage of next damp fruit body, later all by this management.
The method that the glossy ganoderma of described enrichment DNJ composition is cultivated, it is characterized in that, its culture base-material is made up of the raw material of following weight portion: ramulus mori bits 72, mulberry root bits 14, silkworm excrement 3, mulberry leaf powder 3, wheat bran 2, Cottonseed Meal 2, sucrose 1, land plaster 1, pulverized limestone 1, calcium carbonate 0.5.
The method that the glossy ganoderma of described enrichment DNJ composition is cultivated, it is characterized in that step plays that (1) choosing is dry becomes without the belt leather mulberry branch, the Mulberry Roots that go mouldy ramulus mori bits and the mulberry root bits that size is 0.2-0.5 centimetre of left and right after crushed, mulberry leaf crushed after being dried becomes 5-10 object mulberry leaf powder, and silkworm excrement is for subsequent use after dry grinding is shone in screening.
The method that the glossy ganoderma of described enrichment DNJ composition is cultivated, is characterized in that step is played after (2) ramulus mori bits, mulberry root bits in advance mix in proportion 40% left and right that adds water for 2 days and stir to stack, and allows material fully absorb water and to prewet.
The method that the glossy ganoderma of described enrichment DNJ composition is cultivated, it is characterized in that step plays (6) described bacterium rod that will connect kind and move into groined type in baterial cultivation chamber of strict desinsection, sterilization and stack, sterilization adopts containing the chlorine dioxide 400mg/L aqueous solution sprays metope, ground, space and apparatus, or with the chlorine dioxide containing 2000mg/L concentration, by 40ml/m
324 hours are fumigated and sealed to consumption.
The cultural method of the glossy ganoderma of described a kind of enrichment DNJ composition, is characterized in that also including in culture base-material the chelating state compound trace element fertilizer that contains the elements such as iron, copper, manganese, zinc, calcium, magnesium of 0.1-0.5 weight portion.
The present invention has following meaning:
1. this invention is to utilize nutrition organs that in mulberry tree organ, DNJ content is higher as mulberry branch, Mulberry Roots and mulberry leaf powder, also comprise that mulberry leaf are done by the silkworm excrement of the postdigestive gathering multiple nutritional components of silkworm body etc. the glossy ganoderma that main composts or fertilisers of cultivating is cultivated, retaining on the basis of the original medicinal ingredient of glossy ganoderma, by biological transformation, distinctive mulberry tree DNJ composition is enriched in ganoderma lucidum fruitbody, measure by high performance liquid chromatograph, in ganoderma lucidum fruitbody, DNJ content is up to 0.6459%, and mulberry leaf, in mulberry root and mulberry branch dry matter, DNJ content is respectively 0.11%, 0.14% and 0.176%, in silkworm excrement, DNJ composition is 0.113%, the composition of the ganoderma lucidum fruitbody the cultivated DNJ in can enrichment culture material is described in this way, the ganoderma lucidum product of cultivating is in this way suppressing blood sugar, is improving diabetic physique, reduce and substitute significant to the dependence aspect of insulin, the blood sugar reducing health product aspect having no side effect for exploitation provides material, and the raw material of rich content is also provided for extracting the pharmaceutical manufacturer of DNJ medicinal ingredient simultaneously,
2. culture base-material of the present invention is mainly derived from the by product of sericulture, has sufficient raw, wide material sources, cheap feature; cultivate in this way glossy ganoderma; can effectively protect the limited forest resources, prevent excessive and random lumbering, solve bacterium woods contradiction.Because culture technique method is simple, cost is low, instant effect, for silkworm raiser and planting edible mushroom family bringing very large economic benefit;
Culture base-material formula combination of the present invention the necessary nutrient component of growing of glossy ganoderma, increase the chelating state compound trace element fertilizer of elements such as containing iron, copper, manganese, zinc, calcium, magnesium, not only nutrition is more comprehensive, and effectively regulate growing of glossy ganoderma, the glossy ganoderma producing with this formula, mycelial growth is fast and sturdy, other 10 days left and right mycelia in advance of filling a prescription sends out purseful on year-on-year basis, ganoderma lucidum fruitbody cap pork is thick, contain abundant trace element abundant, output improves 10% left and right compared with conventional method.
embodiment
Below in conjunction with embodiment, the invention will be further described:
The method culture base-material formula that a kind of glossy ganoderma of enrichment DNJ composition is cultivated is made up of the raw material of following weight portion:
Ramulus mori bits 72, mulberry root bits 14, silkworm excrement 3, mulberry leaf powder 3, wheat bran 2, Cottonseed Meal 2, sucrose 1, land plaster 1, pulverized limestone 1, calcium carbonate 0.5, (containing the elements such as iron, copper, manganese, zinc, calcium, magnesium) chelating state compound trace element fertilizer 0.5;
The technological process of a kind of method that glossy ganoderma of enrichment DNJ composition is cultivated comprises the following steps successively:
(1) pulverizing of getting the raw materials ready: choosing is dry becomes ramulus mori bits and mulberry root bits that size is 0.2-0.5 centimetre of left and right for subsequent use without the belt leather mulberry branch, the Mulberry Roots that go mouldy after crushed, silkworm excrement is for subsequent use after dry grinding is shone in screening, mulberry leaf crushed after being dried becomes 5-10 object mulberry leaf powder for subsequent use, and other auxiliary material requirement is fresh, nothing is gone mouldy;
(2) major ingredient is prewetted: ramulus mori bits, mulberry root bits to the excellent use of bacterium processed on the same day mix 40% left and right that adds water for 2 days in advance in proportion, and limit adds waterside stirs, and even rear stacking, allows material fully absorb water and to prewet;
(3) spice pack: the ramulus mori bits after prewetting, mulberry root bits fully mix by formula rate with silkworm excrement, mulberry leaf powder, wheat bran, Cottonseed Meal, land plaster, pulverized limestone, calcium carbonate, chelating state compound trace element fertilizer, sucrose is put into container and is dissolved in water, add in pan feeding and stir gradually, then add water, fully mix thoroughly, water content requires to reach 55% left and right, taking hold tight composts or fertilisers of cultivating have washmarking without water droplet as degree; The culture base-material of mixing is packed into according to difference and need to select different bacterium bags, general cm × 0.0045, the 15cm × 55 cm polypropylene plastics bacterium bag that adopts, utilize machine packing, tighten sack, make bacterium rod, accomplish packing consolidation, material and bag wall tight, and check to have or not and draw thin, damaged, thorn broken bag wall, spice packed sterilizing on the same day accomplished without any letup the same day same day, avoided accumulation and spent the night;
(4) sterilizing: the bacterium rod making " well " font in sterilizing kitchen is stacked, be advisable to be no more than 1500 rods, exceeding 1500 rod sterilization times will increase.Normal-pressure sterilization starts, with very hot oven, to make temperature in 3-4 hour, reach 100 DEG C, namely in whole sterilizing kitchen, is all full of steam, and bottom has steam to overflow, and will keep like this temperature 12-15 hour.Whole sterilization process can not interrupt, and when adding hot water when lack of water in boiler, avoids and adds cold water.Sterilizing finishes rear equitemperature and naturally drops to 60 DEG C and following can go out kitchen range, and excess Temperature pocket easily occurs and bursts, and obtain kitchen range process and handle with care, in handbarrow, will pad cotton felt, prevents from puncturing pocket; Cooling place will be cleaned in advance, desinsection, sterilization processing, adopts sulphur fumigation or the chlorine dioxide fumigation containing 2000mg/L concentration, or with bleaching powder sprinkling wall, space and the ground of 1% effective chlorine density.Natural temperature is down in the cooling place that charge bar is moved into after sterilization;
(5) inoculation: inoculation is front to the strict cleaning of transfer room process, sterilization, can adopt containing the chlorine dioxide 400mg/L aqueous solution or containing the bleaching fluid of effective chlorine composition 1% and carry out spray disinfectant, inoculating hood and inoculating appliance thoroughly clean, and inoculating hood and inoculating appliance sterilization select aerial fog disinfectant 4～8g fumigation about 30 minutes; Inoculating appliance, the outer list processing of bacterium rod and inoculator's both hands should adopt 75% alcohol or 0.2% liquor potassic permanganate scouring sterilization; After sterilization finishes, just can beat cave to bacterium rod and inoculate, on bacterium rod, punch with the perforation rod of diameter 2cm, beat 4,2cm deep hole cave for every bag, bacterial classification is cut into the bulk of 2.5 × 2.5cm left and right, fill in cave, bacterial classification access slightly exceeds cave face, and then overcoat layer of polyethylene plastic sack seals; After every a collection of inoculation, handle with care, transfer to baterial cultivation chamber, and clear up in time residue in inoculating hood, carry out ventilation;
(6) Mycelium culture management: will clean baterial cultivation chamber before bacterium rod moves into, desinsection, disinfect, desinsection can adopt 80% DDT EC stifling, sterilization processing adopts with containing the chlorine dioxide 400mg/L aqueous solution, metope, ground, space and apparatus being sprayed, or with the chlorine dioxide containing 2000mg/L concentration, by 40ml/m
324 hours are fumigated and sealed to consumption; Stack connecting groined type in the excellent baterial cultivation chamber that moves into strict desinsection, sterilization of the bacterium of planting, pile high 6-8 layer, carry out Mycelium culture.Cultural hypha roughly can be divided following three periods:
Germination period: after inoculation, before the 10th day, temperature is controlled at 26-28 DEG C, and after 10-20 days, room temperature is controlled at 22-24 DEG C of left and right, running check to note heap temperature, and heap temperature rise, strengthen ventilation;
Vegetative period: inoculate after latter 20 days, temperature should be controlled at 22 DEG C of left and right, in the time that white hypha body grows into diameter 10cm-15cm around inoculation hole, remove outer bagging with ventilate, promote mycelial growth, carry out turning simultaneously, after turning again in 10-15 days, eliminate the bacterium rod of bacteria infection;
In the maturing stage: after inoculation,, after 35-40 days, mycelium progresses into the physiological ripening stage, and room temperature should be controlled at 20-22 DEG C, mainly by opening and closing the door and window adjusting of ventilating, avoid causing burning bacterium because of excess Temperature.When mycelium covers with and sees adularescent projection sesame flower bud after bag, cut the mouth of 2cm left and right in bacterium rod two-port, promote fruit body development.Now can enter Zhi Peng;
(7) management in fruit body stage: selecting has water source, high-land, free of contamination place to build sesame canopy.Sesame canopy is general wide is 6-8 rice, the steel pipe of high 2.5-3 rice or the arched roof of bamboo-wood structure, and length is selected as required.Canopy film is selected white films, and one deck black sunshade net is taken at top.Bacterium rod is wanted strict desinsection, sterilization before entering canopy; Bacterium rod moves into out Zhi Peng while carrying out grand style, by bacterium rod yaw on the ground, reserve operation road, every two bacterium interrod spacings are from 3-5 centimetre, the vegetable garden soil that is rich in humus is filled up at center, on cover the thin sand and soil of 1-2cm, every day is each micro-nozzle intermittent spray shape water 1-2 hour of using sooner or later, keep relative moisture in canopy at 85-95%, within about 7-10 days, to grow with regard to visible ganoderma lucidum fruitbody young sesame flower bud, in the time having multiple sesame flower buds to occur, cut off with sterilization scissors, each bacterium rod is preserved 2-4 sesame flower bud, makes nutrition centralizedly supply, cultivates large thick fruit body of lid.Grow brownish red basidiospore below the growth of cap Edge-stopping, cap time, illustrate that fruit body is ripe, now can gather.While gathering, with pocket knife from stem middle part following cutting, prevent that stem from breaking, the glossy ganoderma airing after harvesting is dried, or in low temperature 55-60 DEG C oven dry.Gather after the 1st tide, the 5-7 days that cuts off the water, after the wound healing causing because of harvesting fruit body, and then is interrupted water spray, keeps relative moisture more than 85%, enters the growth and development stage of next damp fruit body, later all by this management.
Claims (6)
1. the cultural method of the glossy ganoderma of an enrichment DNJ composition, it is characterized in that, comprise the following steps: its culture matrix is made up of the raw material of following weight portion: ramulus mori bits 65-75, mulberry root bits 10-20,, silkworm excrement 1-10, mulberry leaf powder 1-10, wheat bran 1-10, Cottonseed Meal 1-5, sucrose 1-5, land plaster 1-5, pulverized limestone 0.1-1, calcium carbonate 0.1-1;
(1) pulverizing of getting the raw materials ready: choosing is dry for subsequent use after crushed without the mulberry branch, Mulberry Roots, mulberry leaf, the silkworm excrement that go mouldy, that other auxiliary material requires is fresh, without going mouldy;
(2) major ingredient is prewetted: ramulus mori bits, mulberry root bits to bacterium rod use processed mix in advance in proportion, add water and prewet;
(3) spice pack: the ramulus mori bits after prewetting, mulberry root bits fully mix by formula rate with silkworm excrement, mulberry leaf powder, wheat bran, Cottonseed Meal, land plaster, pulverized limestone, calcium carbonate, sucrose is put into container and is dissolved in water, then add and stir gradually, then add water, limit edged stirs, and water content requires to reach 55% left and right; Pack the culture base-material of mixing into bacterium bag, tighten sack, make bacterium rod;
(4) sterilizing: by the bacterium rod making sterilizing 12-15 hour under 100 DEG C of normal pressures, sterilizing finishes rear equitemperature and naturally drops to 60 DEG C of right and lefts and can take the dish out of the pot, and natural temperature is down in the cooling place that charge bar is moved into after cleaning, sterilization;
(5) inoculation: before inoculation,, to the strict cleaning of transfer room process, sterilization, inoculating hood and inoculating appliance thoroughly clean, inoculating hood and inoculating appliance sterilization select aerial fog disinfectant 4～8g fumigation about 30 minutes; Inoculating appliance, the outer list processing of bacterium rod and inoculator's both hands should adopt 75% alcohol or 0.2% liquor potassic permanganate scouring sterilization; After sterilization finishes, just can play cave inoculation to bacterium rod, carry out bagging sealing outward connecting the bacterium rod of planting in time; After inoculation, clear up in time residue in inoculating hood, carry out ventilation;
(6) Mycelium culture management: stack connecting groined type in the excellent baterial cultivation chamber that moves into strict desinsection, sterilization of the bacterium of planting, pile high 6-8 layer, carry out Mycelium culture; After inoculation, 8-10 days temperature are controlled at 25 DEG C of left and right, and after 18-20 days, room temperature is controlled at 23 DEG C of left and right, running check to note heap temperature, heap temperature rise, strengthen ventilation, in the time that white hypha body grows into diameter 12cm-15cm around inoculation hole, remove outer bagging with ventilate, promote growth; After 35-40 days, room temperature is controlled at 20-22 DEG C, and mycelium can, at bacterium rod two ends clip, promote fruit body development when covering with and seeing adularescent projection sesame flower bud after bag, then just can move into out Zhi Peng, carries out grand style;
(7) management in fruit body stage: selecting has water source, high-land, free of contamination place to build sesame canopy, and after strict desinsection, sterilization, bacterium rod just can move into out Zhi Peng and carry out grand style; When grand style, bacterium rod is lain in to ground, bacterium interrod spacing 3-5cm, fill up the vegetable garden soil that is rich in humus, above cover the thin sand and soil of 1-2cm, each 1-2 hour sooner or later every day, utilize micro-nozzle intermittent spray shape water, keep that relative moisture is in 85-95% left and right in canopy, about 7 days, just visible ganoderma lucidum fruitbody young sesame flower bud grows, and cuts off too much Zhi Lei with the scissors of sterilizing, every rod retains 2-4 of sesame flower bud, and ganoderma lucidum fruitbody maturation just can be gathered drying afterwards; Gather after the 1st tide, the 5-7 days that cuts off the water, after the wound healing causing because of harvesting fruit body, and then is interrupted water spray, keeps relative moisture more than 85%, enters the management of the growth and development stage of next damp fruit body, later all by this management.
2. the cultural method of the glossy ganoderma of a kind of enrichment DNJ composition described in, it is characterized in that, its culture base-material is made up of the raw material of following weight portion: ramulus mori bits 72, mulberry root bits 14, silkworm excrement 3, mulberry leaf powder 3, wheat bran 2, Cottonseed Meal 2, sucrose 1, land plaster 1, pulverized limestone 1, calcium carbonate 0.5.
3. described in a kind of cultural method of glossy ganoderma of enrichment DNJ composition, it is characterized in that step plays that (1) choosing is dry becomes without the belt leather mulberry branch, the Mulberry Roots that go mouldy ramulus mori bits and the mulberry root bits that size is 0.2-0.5 centimetre of left and right after crushed, mulberry leaf crushed after being dried becomes 5-10 object mulberry leaf powder, and silkworm excrement is for subsequent use after dry grinding is shone in screening.
4. described in a kind of cultural method of glossy ganoderma of enrichment DNJ composition, it is characterized in that step is played after (2) ramulus mori bits, mulberry root bits in advance mix in proportion 40% left and right that adds water for 2 days and stir to stack, allow material fully absorb water and to prewet.
5. described in a kind of cultural method of glossy ganoderma of enrichment DNJ composition, it is characterized in that step plays (6) described bacterium rod that will connect kind and move into groined type in baterial cultivation chamber of strict desinsection, sterilization and stack, sterilization adopts containing the chlorine dioxide 400mg/L aqueous solution sprays metope, ground, space and apparatus, or with the chlorine dioxide containing 2000mg/L concentration, by 40ml/m
324 hours are fumigated and sealed to consumption.
6. described in a kind of cultural method of glossy ganoderma of enrichment DNJ composition, it is characterized in that also including in culture base-material the chelating state compound trace element fertilizer that contains the elements such as iron, copper, manganese, zinc, calcium, magnesium of 0.1-0.5 weight portion.
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| CN111034538A (en) * | 2019-12-23 | 2020-04-21 | 岳西县思远生态农业有限公司 | Method for culturing black fungus by utilizing mulberry branches |
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