CN104200728A - Up-down filter type carbonic monoxide toxicity test device and method - Google Patents
Up-down filter type carbonic monoxide toxicity test device and method Download PDFInfo
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- CN104200728A CN104200728A CN201410492349.1A CN201410492349A CN104200728A CN 104200728 A CN104200728 A CN 104200728A CN 201410492349 A CN201410492349 A CN 201410492349A CN 104200728 A CN104200728 A CN 104200728A
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- carbon monoxide
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Abstract
The invention discloses an up-down filter type carbonic monoxide toxicity test device and method. The device comprises a carbonic monoxide generator, a temperature adjustable heater and a carbon monoxide poisoning chamber, and the carbonic monoxide generator is disposed on the temperature adjustable heater and connected with the carbon monoxide poisoning chamber through a pipe. The carbonic oxide generator comprises a beaker and a generation container arranged in the beaker, the generation container comprises an inverted cone volumetric apparatus and a conical volumetric apparatus integrally formed, a piston is arranged between the inverted cone volumetric apparatus and the conical volumetric apparatus, and a plug and an escape pipe are arranged at the top end of the generation container. The carbon monoxide poisoning chamber comprises a tank which is internally partitioned into an upper chamber and a lower chamber, an interlayer is arranged between the lower chamber and the upper chamber, the lower chamber is connected with an intake pipe, an air outlet is arranged in the upper end of interlayer, and a plug and a waste pipe are further arranged at the top of the tank. The device is compact and reasonable in structure, simple and feasible in experimental operation, stable in experimental conditions and good in repeatability, and working efficiency and experiment quality can be improved remarkably.
Description
Technical field
The present invention relates to a kind of experimental provision, be specifically related to a kind of device and experimental technique of preparing CO gas and set up mouse anthracemia model simultaneously in laboratory.
Background technology
In college, institution of higher learning and scientific research institution, the experimental study of many relevant specialities all will carry out the experiment of mouse anthracemia at present.Especially in specialties such as chemistry, biological chemistry, medical science, pharmacy, preventive medicine, toxicology and environmental sciences, all need to carry out a large amount of experimental studies relevant with carbon monoxide.The carbon monoxide adopting at present generates with poisoning device (Figure of description 2) and comprises two parts: the one, produce the part of carbon monoxide, comprise iron stand, test tube clamp, separating funnel, band cork flask, spirit lamp etc., the 2nd, mouse anthracemia part, comprises draft tube, two wide-necked bottles, wireway and escape pipes.Traditional experimental provision complex structure, practical operation is loaded down with trivial details, and the temperature of chemical reaction is unstable, and the poor repeatability of experimental result is cleaned inconvenient.
Summary of the invention
Goal of the invention: the object of the invention is in order to solve deficiency of the prior art, a kind of rational in infrastructure, simple to operate and carbon monoxide toxicity test device that experimental repeatability is good is provided.
Technical scheme: a kind of vertical type filter-type carbon monoxide toxicity test device of the present invention, comprise carbon monoxide maker, adjustable temperature heater and anthracemia storehouse, described carbon monoxide maker is placed on described adjustable temperature heater, and described carbon monoxide maker is connected by body with anthracemia storehouse; The generation container that described carbon monoxide maker comprises beaker and is placed in described beaker, described generation container comprises integrated back taper Container and taper Container, between described back taper Container and taper Container, be provided with piston, described generation container top is provided with bottle stopper and escape pipe; Described anthracemia storehouse comprises tank body, is separated into Xia Cang and Shang Cang in described tank body, between described Xia Cang and upper storehouse, is provided with interlayer, and described lower storehouse is connected with draft tube, and described interlayer upper end is provided with venthole, and described tank body top is also provided with bottle stopper and off-gas line.
As optimization, described bottle stopper is frosted bottle stopper.
As optimization, described piston is the frosted piston with holes of 360 ° of rotations.
As optimization, in described beaker, there is water, the temperature of described water is 80 ℃.
As optimization, bottom, described anthracemia storehouse is provided with structure thicker, has strengthened the stability of whole device.
As optimization, the material of described interlayer is glass.
As optimization, in described lower storehouse, sodium hydroxide solution is housed, the concentration of described sodium hydroxide solution is 0.5mol/L.
As optimization, on described interlayer, be placed with animal used as test.
The experimental technique that the invention also discloses above-mentioned vertical type filter-type carbon monoxide toxicity test device, comprises the steps:
(1) by carbon monoxide maker water-bath part preheating and maintain 80 ℃: by the Temperature Setting of adjustable temperature heater at 80 ℃, the glass beaker that suitable quantity of water is housed is placed on well heater, then the taper Container of carbon monoxide maker is partly placed in to beaker;
(2) inject dilute NaOH solution to the lower storehouse in anthracemia storehouse: the dilute NaOH solution that the concentration preparing in advance by draft tube downward storehouse injection is 0.5mol/L;
(3) with conduit, carbon monoxide maker is connected with poisoning storehouse;
(4) place mouse: the bottle stopper of opening poisoning storehouse is put into upper storehouse by the mouse that weighs up body weight;
(5) produce carbon monoxide: pull out the frosted bottle stopper of carbon monoxide maker, open piston, in the back taper Container of head, inject the concentrated sulphuric acid, after the taper Container below the concentrated sulphuric acid flows into completely, closure piston; Then the back taper Container to head adds formic acid, opens piston, allows formic acid flow down and strong sulfuric acid response generates carbon monoxide, and after formic acid flows down completely, quick closedown piston and cover frosted bottle stopper, in order to avoid the CO gas generating outwards overflows;
(6) set up mouse anthracemia model: when opening piston, by formic acid, flow down, allow formic acid and sulfuric acid mix when starting to generate carbon monoxide, cover tightly the bottle stopper in poisoning storehouse, examine the anthracemia course of reaction that records mouse simultaneously.
Beneficial effect: compact conformation of the present invention is reasonable, and experimental implementation is simple, experiment condition is stable, reproducible, can significantly improve work efficiency and quality of experiments.
Accompanying drawing explanation
Fig. 1 is experimental provision structural representation of the present invention.
Fig. 2 is the structural representation of traditional experimental provision.
Embodiment
A kind of vertical type filter-type carbon monoxide toxicity test device as shown in Figure 1, comprise carbon monoxide maker, adjustable temperature heater 7 and anthracemia storehouse, carbon monoxide maker is placed on described adjustable temperature heater 7, and carbon monoxide maker is connected by body with anthracemia storehouse; The generation container that carbon monoxide maker comprises beaker 6 and is placed in described beaker 6, generate container and comprise integrated back taper Container 2 and taper Container 5, between back taper Container 2 and taper Container 5, be provided with can 360 ° of rotations frosted piston 3 with holes, generate container top and be provided with frosted bottle stopper 1 and escape pipe 4; Anthracemia storehouse comprises tank body, is separated into 11He Shang storehouse, lower storehouse 13 in tank body, between 11Yu Shang storehouse, lower storehouse 13, is provided with glass interlayer 12, and lower storehouse 11 is connected with draft tube 8, and interlayer 12 upper ends are provided with venthole 9, and tank body top is also provided with bottle stopper 14 and off-gas line 15.
This toxicity test device is comprised of three parts, i.e. carbon monoxide maker, adjustable temperature heater and anthracemia storehouse.Carbon monoxide maker, compares with the carbon monoxide device (as shown in Figure 2) of producing of traditional split, and it is the airtight of disjunctor.It comprises again four parts: head, neck, body and water-bath part.Head comprises frosted bottleneck, bottle stopper and back taper Container.Neck is comprised of 360 ℃ of rotatable frosted pistons with holes and escape pipe.Body is taper Container, for formic acid and sulfuric acid reaction, generates carbon monoxide.In addition, water-bath is partly the glass beaker that appropriate 80 ℃ of hot water are housed, and taper Container is placed in this water-bath again, for accelerating CO gas formation speed.
Adjustable temperature heater, makes water-bath keep 80 ℃ of constant temperature by its work, strictly controls chemical reaction temperature.
Anthracemia storehouse, is one and has bilevel wide-necked bottle.Its bottle comprises storehouse, narrow meshed interlayer and Xia Cang, and dilute NaOH solution is equipped with in lower storehouse, for filtering carbon monoxide.One draft tube is arranged at its bottle of bottom, and the Yu Xia storehouse, one end of draft tube communicates, and the other end is connected with carbon monoxide maker.When having carbon monoxide to generate, carbon monoxide overflows from the dilute NaOH solution in lower storehouse by draft tube, and carbon monoxide is produced volatile ingredient that in process, other is mingled with and is dissolved in dilute NaOH solution as formic acid.Carbon monoxide enters Shang Cang by the aperture on interlayer, and the density ratio air of carbon monoxide is lower slightly, and carbon monoxide enters Shang Cang from bottom, mixes better with the air in bottle.Upper storehouse is used for placing mouse.Off-gas line in bottle stopper communicates with atmosphere, makes partial pressure of oxygen in anthracemia storehouse and the partial pressure of oxygen balance in atmosphere, thereby has got rid of the factor that partial pressure of inspired oxygen reduces, and the anoxic that makes mouse is to be caused by simple anthracemia.Bottom is provided with structure thicker 10, thereby strengthens the stability of whole device.This device experimental implementation is simple, and experiment condition is stable, reproducible, and the laboratory of the colleges and universities of applicable each related discipline professional domain, scientific research institution etc. is widely used, and can significantly improve work efficiency and quality of experiments.
The specific experiment procedure of this experimental provision is as follows:
(1) by carbon monoxide maker water-bath part preheating and maintain 80 ℃.The Temperature Setting of adjustable temperature heater, at 80 ℃, is placed on the glass beaker that suitable quantity of water is housed on well heater.Again the taper Container of carbon monoxide maker is partly placed in to beaker;
(2) inject dilute NaOH solution to the lower storehouse in anthracemia storehouse.The dilute NaOH solution 10ml that the concentration preparing in advance by injection in the downward storehouse of draft tube is 0.5mol/L;
(3) as Fig. 1 is connected carbon monoxide maker with conduit with poisoning storehouse;
(4) place mouse.Open the bottle stopper in poisoning storehouse the mouse that weighs up body weight is put into upper storehouse;
(5) produce carbon monoxide.Pull out the frosted bottle stopper of carbon monoxide maker, open piston, in the back taper Container of head, inject 95～98% concentrated sulphuric acid 2ml, after the taper Container below the concentrated sulphuric acid flows into completely, closure piston.Then the back taper Container to head adds 88% formic acid 3ml, opens piston, allows formic acid flow down with strong sulfuric acid response and generates carbon monoxide.After formic acid flows down completely, quick closedown piston and cover frosted bottle stopper, in order to avoid the CO gas generating outwards overflows;
(6) set up mouse anthracemia model simultaneously.When opening piston, by formic acid, flow down, allow formic acid and sulfuric acid mix when starting to generate carbon monoxide, cover tightly the bottle stopper in poisoning storehouse, examine the anthracemia course of reaction that records mouse.
This experimental provision invention compact conformation is reasonable, and experimental implementation is simple, and experiment condition is stable, reproducible, can significantly improve work efficiency and quality of experiments.
Claims (9)
1. a vertical type filter-type carbon monoxide toxicity test device, it is characterized in that: comprise carbon monoxide maker, adjustable temperature heater (7) and anthracemia storehouse, it is upper that described carbon monoxide maker is placed on described adjustable temperature heater (7), and described carbon monoxide maker is connected by body with anthracemia storehouse; Described carbon monoxide maker comprises beaker (6) and the generation container that is placed in described beaker (6), described generation container comprises integrated back taper Container (2) and taper Container (5), between described back taper Container (2) and taper Container (5), be provided with piston (3), described generation container top is provided with bottle stopper (1) and escape pipe (4); Described anthracemia storehouse comprises tank body, in described tank body, be separated into lower storehouse (11) and upper storehouse (13), between described lower storehouse (11) and upper storehouse (13), be provided with interlayer (12), described lower storehouse (11) is connected with draft tube (8), described interlayer (12) upper end is provided with venthole (9), and described tank body top is also provided with bottle stopper (14) and off-gas line (15).
2. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: described bottle stopper (1) is frosted bottle stopper.
3. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: described piston (3) is the frosted piston with holes of 360 ° of rotations.
4. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: described beaker has water in (6), and the temperature of described water is 80 ℃.
5. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: bottom, described anthracemia storehouse is provided with structure thicker (10).
6. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: the material of described interlayer (12) is glass.
7. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: in described lower storehouse (11), sodium hydroxide solution is housed, the concentration of described sodium hydroxide solution is 0.5mol/L.
8. a kind of vertical type filter-type carbon monoxide toxicity test device according to claim 1, is characterized in that: described interlayer is placed with animal used as test on (12).
9. an experimental technique for vertical type filter-type carbon monoxide toxicity test device, is characterized in that: comprise the steps:
(1) by carbon monoxide maker water-bath part preheating and maintain 80 ℃: by the Temperature Setting of adjustable temperature heater at 80 ℃, the glass beaker that suitable quantity of water is housed is placed on well heater, then the taper Container of carbon monoxide maker is partly placed in to beaker;
(2) inject dilute NaOH solution to the lower storehouse in anthracemia storehouse: the dilute NaOH solution that the concentration preparing in advance by draft tube downward storehouse injection is 0.5mol/L;
(3) with conduit, carbon monoxide maker is connected with poisoning storehouse;
(4) place mouse: the bottle stopper of opening poisoning storehouse is put into upper storehouse by the mouse that weighs up body weight;
(5) produce carbon monoxide: pull out the frosted bottle stopper of carbon monoxide maker, open piston, in the back taper Container of head, inject the concentrated sulphuric acid, after the taper Container below the concentrated sulphuric acid flows into completely, closure piston; Then the back taper Container to head adds formic acid, opens piston, allows formic acid flow down and strong sulfuric acid response generates carbon monoxide, and after formic acid flows down completely, quick closedown piston and cover frosted bottle stopper, in order to avoid the CO gas generating outwards overflows;
(6) set up mouse anthracemia model: when opening piston, by formic acid, flow down, allow formic acid and sulfuric acid mix when starting to generate carbon monoxide, cover tightly the bottle stopper in poisoning storehouse, examine the anthracemia course of reaction that records mouse simultaneously.
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| CN201410492349.1A CN104200728B (en) | 2014-09-24 | 2014-09-24 | A kind of carbon monoxide poisoning experimental provision of vertical type filter-type |
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| CN201410492349.1A CN104200728B (en) | 2014-09-24 | 2014-09-24 | A kind of carbon monoxide poisoning experimental provision of vertical type filter-type |
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| CN104200728B CN104200728B (en) | 2016-11-30 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU674083A1 (en) * | 1976-07-21 | 1979-07-15 | Ростовский государственный медицинский институт | Educational appliance on medicine |
| CN101352586A (en) * | 2008-08-26 | 2009-01-28 | 程树军 | Method for preparing full-thickness skin for toxicity test by stem cell raft type cultivation |
| US20090029331A1 (en) * | 2007-06-12 | 2009-01-29 | Crawford Gregory P | Active cutaneous technology |
| CN203673734U (en) * | 2013-05-30 | 2014-06-25 | 王江龙 | Environment-friendly type demonstration apparatus for experiment of reducing copper oxide with carbon monoxide |
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2014
- 2014-09-24 CN CN201410492349.1A patent/CN104200728B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU674083A1 (en) * | 1976-07-21 | 1979-07-15 | Ростовский государственный медицинский институт | Educational appliance on medicine |
| US20090029331A1 (en) * | 2007-06-12 | 2009-01-29 | Crawford Gregory P | Active cutaneous technology |
| CN101352586A (en) * | 2008-08-26 | 2009-01-28 | 程树军 | Method for preparing full-thickness skin for toxicity test by stem cell raft type cultivation |
| CN203673734U (en) * | 2013-05-30 | 2014-06-25 | 王江龙 | Environment-friendly type demonstration apparatus for experiment of reducing copper oxide with carbon monoxide |
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