CN104172200B - A kind of improve the method for soluble dietary fibre content in pomace - Google Patents
A kind of improve the method for soluble dietary fibre content in pomace Download PDFInfo
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- CN104172200B CN104172200B CN201410352727.6A CN201410352727A CN104172200B CN 104172200 B CN104172200 B CN 104172200B CN 201410352727 A CN201410352727 A CN 201410352727A CN 104172200 B CN104172200 B CN 104172200B
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 60
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 78
- 238000012545 processing Methods 0.000 claims abstract description 8
- 238000004090 dissolution Methods 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 41
- 230000004048 modification Effects 0.000 claims description 9
- 238000012986 modification Methods 0.000 claims description 9
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000013517 stratification Methods 0.000 claims description 3
- 238000007781 pre-processing Methods 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 abstract description 8
- 239000001913 cellulose Substances 0.000 abstract description 6
- 229920002678 cellulose Polymers 0.000 abstract description 6
- 239000001814 pectin Substances 0.000 abstract description 5
- 235000010987 pectin Nutrition 0.000 abstract description 5
- 229920001277 pectin Polymers 0.000 abstract description 5
- 210000000170 cell membrane Anatomy 0.000 abstract description 4
- 210000002421 cell wall Anatomy 0.000 abstract description 4
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920002521 macromolecule Polymers 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- 150000005846 sugar alcohols Polymers 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000010525 oxidative degradation reaction Methods 0.000 abstract description 3
- 238000002715 modification method Methods 0.000 abstract description 2
- 229960002163 hydrogen peroxide Drugs 0.000 description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 17
- 230000008569 process Effects 0.000 description 15
- 239000000835 fiber Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 235000012054 meals Nutrition 0.000 description 5
- 238000012869 ethanol precipitation Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000000643 oven drying Methods 0.000 description 4
- 230000000630 rising effect Effects 0.000 description 4
- -1 dimension element Polymers 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000008236 heating water Substances 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 238000002845 discoloration Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
nullThe invention discloses and a kind of improve the method for soluble dietary fibre content in pomace，Hydrogen peroxide is utilized to have oxidative degradation in the basic conditions，Pending pomace is mixed with certain density alkaline hydrogen peroxide solution，Hydrogen peroxide is made to destroy the cross-linked structure of pomace cell wall polysaccharides in the basic conditions，Promote pectin in cell membrane、The soluble dietary fiber dissolutions such as solubility hemicellulose，And make cellulose、The glycosidic bond fracture of the indissoluble macromolecule polyalcohols such as insolubility hemicellulose，Occur modified thus be converted into soluble dietary fiber，The soluble dietary fibre content making pomace rises to 31.51% from original 3.30%，And the soluble dietary fibre content after processing pomace apparently higher than employing ferment treatment method at present or general physical modification method，There is low cost、Technique is simple and soluble dietary fibre content such as significantly improves at the advantage.
Description
Technical field
The present invention relates to biological technical field, particularly relate to soluble dietary fiber in a kind of raising pomace and contain
The method of amount.
Background technology
Pomace is AJC processing byproduct, and China's annual production is the hugest, up to 3,000,000 tons/
Year.At present most domestic AJC enterprise does not the most directly utilize pomace, and as feed,
Fertilizer is sold or as garbage disposal, surcharge is extremely low, and easily causes environmental pollution, therefore pomace
Comprehensive utilization had become as a problem demanding prompt solution.Pomace becomes rich in various plants nutrition
Point, such as pectin, polyphenol, dietary fiber etc., especially dietary fiber, content can reach dry
More than 60%, it is a kind of good dietary fiber sources.
Dietary fiber is the seventh-largest nutrients of the mankind, can be divided into soluble dietary fiber by its dissolubility
(soluble dietary fibre, SDF) and insoluble dietary fiber (insoluble dietary fibre,
IDF).Research finds, the content of SDF is the key factor affecting physiological function of diet fiber.With
IDF compares, and SDF is more easy to, by gut flora fermentating metabolism, reduce blood glucose response and plasma cholesterol.Beautiful
State Leitz etc. propose, and SDF accounts for more than TDF10% could be referred to as high-quality dietary fiber.Also scholar is had to recognize
For, its SDF/IDF ratio of dietary fiber being suitable as food composition should be close to 1: 2.But pomace
Middle SDF content does not far reach this ratio, therefore increases apple by specific treatment technology means
In slag, the content of SDF has highly important theory significance and economic worth.
At present, the method for modifying of dietary fiber mainly has Physical, chemical method and bioanalysis.Forefathers are by squeezing
The methods such as the process of pressure technology, superhigh pressure technique, superfine communication technique, soda acid, enzymolysis, fermentation, make fibre
The glycosidic bond fracture of the indissoluble macromolecule polyalcohols such as dimension element, hemicellulose, changes into readily soluble small molecular sugar
Class, improves the content of SDF.But, in these method of modifying at present, there is also several comparison and dash forward
The problem gone out, physical modification generally requires purchases special instrument and equipment, and chemical modification effect is poor and deposits
Remain at reagent, enzyme modification high cost.Therefore still need to set up new method of modifying.
Summary of the invention
It is an object of the invention to provide and a kind of improve the method for soluble dietary fibre content in pomace, utilize
Hydrogen peroxide has oxidative degradation in the basic conditions, by pending pomace and certain density alkali
Property hydrogenperoxide steam generator mixing, make hydrogen peroxide destroy the friendship of pomace cell wall polysaccharides in the basic conditions
Connection structure, promotes the soluble dietary fiber dissolution such as pectin, solubility hemicellulose in cell membrane, and makes
The glycosidic bond fracture of the indissoluble macromolecule polyalcohols such as cellulose, insolubility hemicellulose, occur modified from
And it is converted into soluble dietary fiber, it is greatly increased the content of soluble dietary fiber in pomace.
It is a further object of the invention that hydrogen peroxide has discoloration, alkalescence peroxide in the basic conditions
Change hydrogen can the chromophore being combined on pomace dietary fibers be bleached, thus improve containing abundant
The organoleptic quality of dietary fiber pomace.
The technical scheme is that
A kind of improve the method for soluble dietary fibre content in pomace, wherein,
The alkaline hydrogen peroxide solution that pending pomace and concentration are 1-2% is mixed according to mass ratio 1: 18-22
Merge heating, make soluble dietary fiber dissolution and insoluble dietary fiber generation modification be pre-processed
Pomace, wherein heating-up temperature 60-70 DEG C, heat time 1.5-2h；
Additionally, further comprising the steps of:
Step one, by the pH value of HCl solution regulation gained pretreatment pomace to 6-7, and with a certain amount of
Ethanol mixing after stratification；
Step 2, taking precipitate are dried, and pulverize, and have both obtained the modification that soluble dietary fibre content improves
Pomace.
Preferably, described improves in pomace in the method for soluble dietary fibre content, described alkalescence
The pH value of hydrogen peroxide is 10-12.
Preferably, in described raising pomace in the method for soluble dietary fibre content, in step one
Described pretreatment pomace is 1: 3-4 with the volume ratio of ethanol.
Preferably, described improves in pomace in the method for soluble dietary fibre content, ethanol used
For 90-100% ethanol.
Preferably, described improves in pomace in the method for soluble dietary fibre content, described pre-place
Reason pomace is 1.5-2h with the mixed processing time of ethanol.
Preferably, in described raising pomace in the method for soluble dietary fibre content, in step 2
Baking temperature is 60-70 DEG C, is dried to pomace moisture and is not higher than 6%
Preferably, in described raising pomace in the method for soluble dietary fibre content, in step 2
Dried precipitated powder is broken to below 0.45mm, and crosses at least 40 mesh sieves.
Preferably, described improves in pomace in the method for soluble dietary fibre content, described alkalescence
The concentration of hydrogen peroxide is 1.67%, and pH value is 11, and described pending pomace mixes with alkaline hydrogen peroxide
Compound heating-up temperature is 70 DEG C, heat time 2h.
Preferably, described improves in pomace in the method for soluble dietary fibre content, ethanol used
Being 95% ethanol, pretreatment pomace is 2h with the mixed processing time of ethanol, pretreatment pomace and second
The volume ratio of alcohol is 1: 4.
The method have the advantages that utilizing hydrogen peroxide to have oxidative degradation in the basic conditions makees
With, pending pomace is mixed with certain density alkaline hydrogen peroxide solution, makes hydrogen peroxide at alkali
Destroy the cross-linked structure of pomace cell wall polysaccharides under the conditions of property, promote pectin in cell membrane, solubility half
The soluble dietary fiber dissolutions such as cellulose, and make the indissoluble such as cellulose, insolubility hemicellulose divide greatly
The glycosidic bond fracture of sub-polymer, occurs modified thus is converted into soluble dietary fiber, be greatly increased apple
The content of soluble dietary fiber in pomace.Through testing inspection, the method for the invention is utilized to process apple
After pomace, in pomace, soluble dietary fibre content is 31.51%, and the yield of soluble dietary fiber is
22.28%, and in undressed pomace, soluble dietary fibre content is only 3.30%, solubility meals
The yield of food fiber is less than 3%, it is seen that the method for the invention can make soluble dietary fiber in pomace
Content is greatly enhanced.Enzyme process or general physical modification method is used to process pomace not at present
Only cost is high, and equipment is complicated, and in the pomace after processing, soluble dietary fibre content is the highest
In 20%, the effect reached well below the method for the invention.
Further, since hydrogen peroxide has discoloration in the basic conditions, can be to being combined in pomace meals
Chromophore on food fiber is bleached, thus improves the organoleptic quality containing abundant dietary fiber pomace.
Meanwhile, hydrogen peroxide is the oxidant of a kind of clean and effective, can be decomposed into oxygen and water, noresidue.
The present invention has low cost, and technique is simple, it is not necessary to the advantage such as special installation and noresidue, and generally applicable
Comprehensive utilization in garden stuff processing accessory substance.
Accompanying drawing explanation
Fig. 1 is the method technological process of soluble dietary fibre content in raising pomace of the present invention
Figure；
Fig. 2 is that in the present invention, pH value affects schematic diagram to modified pomace dry yield；
Fig. 3 be in the present invention pH value to soluble dietary fibre content and solubility meals in modified pomace
That eats fiber yield affects schematic diagram；
Fig. 4 is the hydrogenperoxide steam generator concentration impact signal on modified pomace dry yield in the present invention
Figure；
Fig. 5 be in the present invention hydrogenperoxide steam generator concentration to soluble dietary fibre content in modified pomace
And soluble dietary fiber yield affect schematic diagram；
Fig. 6 is that in the present invention, treatment temperature affects schematic diagram to modified pomace dry yield；
Fig. 7 be in the present invention treatment temperature to soluble dietary fibre content and solubility in modified pomace
Dietary fiber yield affect schematic diagram；
Fig. 8 is to process the time in the present invention modified pomace dry yield is affected schematic diagram；
Fig. 9 is to process the time in the present invention to soluble dietary fibre content and solubility in modified pomace
Dietary fiber yield affect schematic diagram.
Detailed description of the invention
The present invention is elaborated by 1-9 below in conjunction with the accompanying drawings, to make those of ordinary skill in the art refer to this
Can implement according to this after specification.
The method of soluble dietary fibre content in pomace is improved as it is shown in figure 1, a kind of, wherein,
The alkaline hydrogen peroxide solution that pending pomace and concentration are 1-2% is mixed according to mass ratio 1: 18-22
Merge heating, make soluble dietary fiber dissolution and insoluble dietary fiber generation modification be pre-processed
Pomace, wherein, through verification experimental verification, heating-up temperature 60-70 DEG C, optimum is 70 DEG C, the heat time
1.5-2h, optimum is 2h.The principle making Soluble Fiber cellulose content in pomace improve is: hydrogen peroxide
Destroy the cross-linked structure of pomace cell wall polysaccharides in the basic conditions, promote pectin in cell membrane, solvable
The soluble dietary fiber dissolutions such as property hemicellulose, and make the indissoluble such as cellulose, insolubility hemicellulose
The glycosidic bond fracture of macromolecule polyalcohol, occurs modified thus is converted into soluble dietary fiber, increase
Add the content of soluble dietary fiber in pomace.
Additionally, further comprising the steps of:
Step one, by the pH value of HCl solution regulation gained pretreatment pomace to 6-7, and with a certain amount of
Ethanol mixing after stratification；
Step 2, by filter remove supernatant, taking precipitate drying box is dried, pulverizer pulverize,
Both the modified pomace that soluble dietary fibre content improves had been obtained.
Described improves in pomace in the method for soluble dietary fibre content, described alkaline hydrogen peroxide
PH value is 10-12, can first configure the hydrogenperoxide steam generator of 1-2% concentration, then adjust with sodium hydroxide solution
Joint pH value, to 10-12, both must be applicable to the alkaline hydrogen peroxide solution of the present invention.The peroxidating of described alkalescence
The concentration of hydrogen is 1.67%, and pH value is 11, and described pending pomace adds with alkaline hydrogen peroxide mixture
Hot temperature is 70 DEG C, and during heat time 2h, effect is optimum.
Described improves in pomace in the method for soluble dietary fibre content, pre-processes described in step one
Pomace is 1: 3-4 with the volume ratio of ethanol, and preferred volume ratio is 1: 4, and ethanol used is 90-100% second
Alcohol, preferably 95% ethanol, described pretreatment pomace is 1.5-2h with the mixed processing time of ethanol, excellent
Select 2h.
Described improves in pomace in the method for soluble dietary fibre content, and in step 2, baking temperature is
60-70 DEG C, preferably 60 DEG C, typically at least 8 hours drying time, make pomace the driest, the most dry
Dry it is not higher than 6% to pomace moisture.
Described improves in pomace in the method for soluble dietary fibre content, by dried in step 2
Precipitated powder is broken to below 0.45mm, and crosses at least 40 mesh sieves.
By tests below, the present invention determines that differential responses condition is to pomace dry yield, soluble dietary
Fiber content and the impact of soluble dietary fiber yield.
(1) pH value is to modified pomace dry yield, soluble dietary fibre content and soluble dietary
The impact of fiber yield, as shown in Figure 2 and Figure 3.
Compound concentration is the hydrogenperoxide steam generator of 1.67%, its pH value is regulated respectively with sodium hydroxide solution
To 10,10.5,11,11.5,12,12.5.Weigh a certain amount of pomace, according to 1: 20 mass score
Do not add the alkaline hydrogen peroxide solution of each different pH value, and carry out following process respectively: 60 DEG C of water
Bath heating 1.5h, takes out and is cooled to room temperature, by HCl solution regulation pH value to 6-7.With 4 times of volumes
95% ethanol precipitation 2h.Filtering, gained filter residue is in 60 DEG C of oven dryings.Pulverize, prepare modified pomace.
After measured, in the basic conditions, along with the rising of hydrogenperoxide steam generator pH value, modified pomace is done
Material yield is gradually reduced.When pH value is 10-11.5, pomace dry yield declines relatively slow, when
When pH value is more than 11.5, dry yield declines very fast.When pH value is 10-11, soluble dietary
Fiber content, soluble dietary fiber yield increase very fast, when pH value is more than 11, and soluble dietary
Fiber content increase is relatively slow, and soluble dietary fiber yield tends towards stability, and therefore, selecting pH value is 11
As optimum pH.
(2) hydrogenperoxide steam generator concentration to modified pomace dry yield, soluble dietary fibre content and
The impact of soluble dietary fiber yield, as shown in Figure 4, Figure 5.
Respectively compound concentration be 0.33%, 1%, 1.67%, 2.33%, 3% and 3.67% hydrogen peroxide molten
Liquid, and with NaOH, solution ph is regulated to 11 respectively.Weigh a certain amount of pomace, according to 1: 20
Mass ratio is separately added into alkaline hydrogen peroxide solution, and processes according to method once respectively: 60 DEG C of water-baths add
Hot 1.5h, takes out and is cooled to room temperature, by HCl solution regulation pH value to 6-7.By 4 times of volume 95% second
Alcohol precipitation 2h.Filtering, gained filter residue, in 60 DEG C of oven dryings, is pulverized, and prepares modified pomace.
After measured, when hydrogenperoxide steam generator concentration is at 0.33-3%, along with the increasing of hydrogenperoxide steam generator concentration
Adding, dry yield is more mild, has downward trend after concentration is 3%.Soluble dietary fibre content,
Soluble dietary fiber yield increases along with the increase of hydrogenperoxide steam generator concentration.Work as hydrogenperoxide steam generator
Concentration is when 0.33-1.67%, and both increase comparatively fast, when hydrogenperoxide steam generator concentration is higher than 1.67%,
Both increase relatively slow, therefore, select 1.67% as optium concentration.
(3) treatment temperature is to modified pomace dry yield, soluble dietary fibre content and solubility meals
The impact of food fiber yield, as shown in Figure 6, Figure 7.
Compound concentration is the hydrogenperoxide steam generator of 1.67%, regulates pH value to 11 with sodium hydroxide solution.
Weigh a certain amount of pomace, add alkaline hydrogen peroxide solution according to 1: 20 mass ratio, respectively at 30
DEG C, 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C of heating water bath 1.5h, take out and be cooled to room temperature, respectively
Process according to method once: by HCl solution regulation pH value to 6-7, by 4 times of volume 95% ethanol precipitations
2h.Filtering, filter residue, in 60 DEG C of oven dryings, is pulverized, and prepares modified pomace.
After measured, along with the rising for the treatment of temperature, pomace dry yield is gradually reduced, and works as treatment temperature
During higher than 70 DEG C, dry yield declines very fast.Along with the rising for the treatment of temperature, soluble dietary fiber
Content is gradually increasing, and tends towards stability when treatment temperature is increased to 70 DEG C.Soluble dietary fiber yield with
The rising temperature gradually rises, and reaches the highest at 70 DEG C, has downward trend subsequently, therefore, selects 70
DEG C as optimum treatmenting temperature.
(4) time that processes is to modified pomace dry yield, soluble dietary fibre content and solubility meals
The impact of food fiber yield, as shown in Figure 8, Figure 9.
Compound concentration is the hydrogenperoxide steam generator of 1.67%, regulates pH value to 11 with sodium hydroxide solution,
Weigh a certain amount of pomace, add alkaline hydrogen peroxide solution according to 1: 20 mass ratio, respectively at 70
DEG C heating water bath 0.5h, 1h, 1.5h, 2h, 3h, 4h, and process according to method once respectively: take out
It is cooled to room temperature, regulates pH to 6-7 with HCl solution, with 4 times of volume 95% ethanol precipitation 2h.Cross
Filter, filter residue, in 60 DEG C of oven dryings, is pulverized, and prepares modified pomace.
After measured, along with process the time prolongation, pomace dry yield is gradually reduced, when treated between
During less than 1h, soluble dietary fibre content and soluble dietary fiber yield, almost without change, work as place
When the reason time is 1h-2h, soluble dietary fibre content increases very fast, due at this stage dry
Yield declines, so soluble dietary fiber yield increase is relatively slow, when treated between more than 2h, solubility
Dietary fiber content and soluble dietary fiber yield are gradually reduced, therefore, during using 2h as optimization process
Between.
Described pomace dry yield, soluble dietary fibre content and the survey of soluble dietary fiber yield
Determine method as follows:
(1) assay method of pomace dry yield:
Precision weighs 2g pomace sample, adds 40mL alkaline hydrogen peroxide solution.According to institute of the present invention
The processing method stated prepares modified pomace, records the quality of dried residue, by dry yield meter
Calculate formula and be calculated pomace dry yield, wherein W1For sample quality；W2For mass of residue,
Described dry yield computing formula is:
(2) assay method of soluble dietary fibre content:
Use AOAC.993.21 improve non-enzymatic gravimetric detemination, precision weigh three parts of each 0.5g of sample in
In 50mL centrifuge tube, add 20mL distilled water, in 60 DEG C of water-bath vibration heating 2h.After cooling,
8000r/min is centrifuged 15min, is poured into by supernatant in 100mL triangular flask, adds 4 times of volumes 95%
Ethanol precipitation 2h.Adding the pickling diatomite of about 0.5g in G2 sand core crucible, 105 DEG C are dried to perseverance
Weight.It is fully transferred to alcohol-insoluble substance fill in diatomaceous crucible, suction filtration.Use 15mL78% the most respectively
Ethanol, 95% ethanol, acetone respectively wash residue twice.105 DEG C of drying.Take a sample determination albumen,
Take another part of sample determination ash content.Protein determination uses the detection of GB 5009.5-2010 method, and ash content uses
GB-5009.4-2010 method detects.Calculated by soluble dietary fibre content computing formula, wherein R1、
R2、R3It is respectively three parts of sample mass of residue (mg)；S1、S2、S3It is respectively three parts of sample qualities (mg)；
P is protein quality (mg) in residue；A is ash content quality (mg) in residue.Described soluble dietary fiber
Cubage formula is:
Soluble dietary fiber yield is to evaluate the index of modification efficiency, and it is used for the modified apple of overall merit
Slag dry yield and soluble dietary fibre content.
Embodiment 1
Step one, the preparation of alkaline hydrogen peroxide, compound concentration is the hydrogenperoxide steam generator of 1.67%, uses hydrogen
Sodium hydroxide solution regulation pH value is 11, has both obtained alkaline hydrogen peroxide solution needed for the present invention.
Step 2, pending pomace is mixed laggard with alkaline hydrogen peroxide solution according to mass ratio 1: 20
70 DEG C of heating water baths of row, heat time 2h, obtain modified pomace.
Step 3, modification pomace is cooled to room temperature, regulates its pH value to 6-7 with HCl solution, and
Mix with 95% ethanol of 4 times of volumes, stand 2h and produce precipitation and be layered.
Step 4, through filtration remove supernatant, by gained sediment in 60 DEG C of baking ovens be dried one timing
Pulverize through pulverizer after between, prepare the pomace that soluble dietary fibre content improves, through test determination,
Gained modification pomace dry yield is 70.69%, and in modified pomace, soluble dietary fibre content can
Reaching 31.51%, the yield of soluble dietary fiber is 22.28%.
Although embodiment of the present invention are disclosed as above, but it is not restricted to specification and embodiment
Using listed by, it can be applied to various applicable the field of the invention completely, for being familiar with this area
Personnel for, be easily achieved other amendment, therefore without departing substantially from claim and equivalency range
Under the universal limited, the present invention is not limited to specific details and shown here as the legend with description.
Claims (5)
1. one kind is improved the method for soluble dietary fibre content in pomace, it is characterised in that
It is that the alkaline hydrogen peroxide solution of 1.67% is according to mass ratio 1:18-22 by pending pomace and concentration
It is mixed and heated, makes soluble dietary fiber dissolution and insoluble dietary fiber generation modification obtain pre-place
Reason pomace, wherein, the pH value of described alkaline hydrogen peroxide is 11, and heating-up temperature is 70 DEG C, heating
Time is 2h；
Additionally, further comprising the steps of:
Step one, by the pH value of HCl solution regulation gained pretreatment pomace to 6-7, and with a certain amount of
Ethanol mixing after stratification；
Step 2, taking precipitate are dried, and pulverize, and obtain the modification that soluble dietary fibre content improves
Pomace.
2. the method for soluble dietary fibre content in raising pomace as claimed in claim 1, it is special
Levying and be, the volume ratio pre-processing pomace and ethanol described in step one is 1:3-4.
3. the method for soluble dietary fibre content in raising pomace as claimed in claim 2, it is special
Levying and be, described pretreatment pomace is 1.5-2h with the mixed processing time of ethanol.
4. the method for soluble dietary fibre content in raising pomace as claimed in claim 3, it is special
Levying and be, in step 2, baking temperature is 60-70 DEG C, is dried to pomace moisture and is not higher than 6%.
5. the method for soluble dietary fibre content in raising pomace as claimed in claim 4, it is special
Levy and be, dried precipitated powder is broken to below 0.45mm by step 2, and crosses at least 40 mesh sieves.
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| CN107212424A (en) * | 2016-05-03 | 2017-09-29 | 上海交通大学 | A kind of extracting method of water-soluble barley diet fiber |
| CN106418322B (en) * | 2016-09-22 | 2019-07-12 | 河南科技学院 | A kind of method of soluble dietary fibre content in raising apple pomace |
| CN109793243A (en) * | 2017-11-16 | 2019-05-24 | 镇江市丹徒区南山溪园茶叶专业合作社 | A kind of processing method that microwave assisting method extracts apple skin soluble dietary fiber |
| CN110477403A (en) * | 2019-08-22 | 2019-11-22 | 华南理工大学 | A method of citrus fruit fibres are prepared using citrus peels |
| CN111838682A (en) * | 2020-07-29 | 2020-10-30 | 河南科技大学 | Preparation method of soluble mung bean hull dietary fiber |
| CN111748047B (en) * | 2020-07-29 | 2022-03-29 | 河南科技大学 | Preparation method of dioscorea opposita oligosaccharide |
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