A kind of compositions and application thereof containing adenosine cyclophosphate
Technical field
The present invention relates to new drug development technical field, relate in particular to the exploitation for the treatment of of myocardial infarction medicine, be specifically related to a kind of compositions and application thereof containing adenosine cyclophosphate.
Background technology
Myocardial infarction is that coronary artery is acute, the caused impatient necrosis of persistence Hypoxia and ischemia.Because of modern live custom change, the appearance of the problems such as the quickening of rhythm of life, the sickness rate of China recent years myocardial infarction is obvious ascendant trend, annual new at least 50 ten thousand, now suffer from least 200 ten thousand, therefore, be devoted to research and develop new effective treatment of myocardial infarction medicine, tool has very important significance and wide market prospect.
Adenosine cyclophosphate, chemical name is 6-amino-9-β-D-RIBOSE base-9H-purine-4`, 5`-cycli phosphate hydrogen ester, molecular formula is C
10h
12n
5o
6p is white or off-white powder; Adenosine cyclophosphate is as the second message,second messenger's material that participates in regulating cell function, and its effect is very extensive, injects heavy dose of adenosine cyclophosphate, can make myocardial contraction strengthen, and causes elevation of the blood pressure, and cardiac output increases.And can diastole smooth muscle, coronary artery dilator blood vessel, improve liver function, suppress the division of skin outer layer epithelial cell and transform paracytic function, promote the oxidasic activity of respiratory chain and improve myocardial ischemia etc.
Diphenhydramine, molecular formula is C
17h
21nOHCl is white crystalline powder; Diphenhydramine is antihistaminic, has the effect of antihistaminic H1 receptor, and nervus centralis is had to stronger inhibitory action.
Vinpocetine, molecular formula is C
22h
26n
2o
2, be white loose block or powder, be usually used in improving the various symptoms that cerebral infarction sequela, apoplexy sequela, cerebral arteriosclerosis etc. are brought out.
Nicotinic acid, also referred to as vitamin B
3, molecular formula is C
6h
5nO
2, colourless acicular crystal, has stronger expansion peripheral vessels effect, clinical headache, migraine, tinnitus, the auditory vertigo etc. of being used for the treatment of.
At present, there is not yet the correlational study for treatment of myocardial infarction by diphenhydramine, vinpocetine, also do not have the correlational study report that diphenhydramine, vinpocetine and nicotinic acid collocation adenosine cyclophosphate is used for the treatment of to myocardial infarction.
Summary of the invention
The object of this invention is to provide a kind of compositions myocardial infarction, that contain adenosine cyclophosphate that is used for the treatment of.
Another object of the present invention is to provide the application of above-mentioned composition in preparation treatment myocardial infarction medicine.
Above-mentioned purpose of the present invention is achieved by following scheme:
Containing a compositions for adenosine cyclophosphate, the formula of said composition is made up of the each component of following parts by weight:
The preferred version of combinations thereof composition formula is as follows:
The preparation method of the above-mentioned compositions containing adenosine cyclophosphate is as follows:
Adenosine cyclophosphate, diphenhydramine, vinpocetine and nicotinic acid are ground respectively and crossed after 100 mesh sieves, according to formula consumption, mix, then put into dry 30 minutes of the baking oven of 55-60 DEG C, prepare the compositions containing adenosine cyclophosphate of the present invention.
The above-mentioned compositions containing adenosine cyclophosphate can be made various dosage forms with pharmaceutically acceptable carrier or excipient, as tablet, granule, capsule or injection etc.
Compositions containing adenosine cyclophosphate of the present invention, through experimentation, has good treatment myocardial infarction effect, therefore, can be used for the medicine of preparation treatment myocardial infarction.
Compared with prior art, the present invention has following beneficial effect:
1. the compositions containing adenosine cyclophosphate of the present invention, introduces diphenhydramine, vinpocetine in the research for the treatment of myocardial infarction medicine first, by by adenosine cyclophosphate, diphenhydramine, vinpocetine and nicotinic acid compatibility, has played the effect for the treatment of well myocardial infarction;
2. the compositions containing adenosine cyclophosphate of the present invention, in its formula, each composition is all that basic research and clinical practice confirm there is no obvious toxic-side effects, and between each composition, there is no the taboo of compatibility, without ill effect mutually, therefore has safety.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is done further and described, but specific embodiment does not do any restriction to the present invention.
Embodiment 1
Compositions containing adenosine cyclophosphate of the present invention, the formula of said composition is made up of the each component of following parts by weight:
The preparation method of the above-mentioned compositions containing adenosine cyclophosphate is as follows:
Adenosine cyclophosphate, diphenhydramine, vinpocetine and nicotinic acid are ground respectively and crossed after 100 mesh sieves, according to formula consumption, mix, then put into dry 30 minutes of the baking oven of 55-60 DEG C, prepare the compositions containing adenosine cyclophosphate of the present embodiment.
Embodiment 2
Compositions containing adenosine cyclophosphate of the present invention, the formula of said composition is made up of the each component of following parts by weight:
The preparation method of the above-mentioned compositions containing adenosine cyclophosphate is as follows:
Adenosine cyclophosphate, diphenhydramine, vinpocetine and nicotinic acid are ground respectively and crossed after 100 mesh sieves, according to formula consumption, mix, then put into dry 30 minutes of the baking oven of 55-60 DEG C, prepare the compositions containing adenosine cyclophosphate of the present embodiment.
Embodiment 3
Compositions containing adenosine cyclophosphate of the present invention, the formula of said composition is made up of the each component of following parts by weight:
The preparation method of the above-mentioned compositions containing adenosine cyclophosphate is as follows:
Adenosine cyclophosphate, diphenhydramine, vinpocetine and nicotinic acid are ground respectively and crossed after 100 mesh sieves, according to formula consumption, mix, then put into dry 30 minutes of the baking oven of 55-60 DEG C, prepare the compositions containing adenosine cyclophosphate of the present embodiment.
Comparative example 1
The compositions of this comparative example, its formula is made up of the each component of following parts by weight:
8 parts of diphenhydramine;
6 parts of vinpocetines;
2 parts, nicotinic acid.
The preparation method of above-mentioned composition is as follows:
Diphenhydramine, vinpocetine and nicotinic acid are ground respectively and crossed after 100 mesh sieves, according to formula consumption, mix, then put into dry 30 minutes of the baking oven of 55-60 DEG C, prepare the compositions of this comparative example.
Embodiment 4 myocardial infarction model experiments
Taking mice as experimental subject, every group of 10 mices, establish 6 groups altogether, specific as follows shown in:
(1) blank group: experimental drug is water for injection.
(2) experimental group 1: experimental drug is a kind of compositions containing adenosine cyclophosphate prepared by embodiment 1;
(3) experimental group 2: experimental drug is a kind of compositions containing adenosine cyclophosphate prepared by embodiment 2;
(4) experimental group 3: experimental drug is a kind of compositions containing adenosine cyclophosphate prepared by embodiment 3;
(5) matched group 1: experimental drug is compositions prepared by comparative example 1;
(6) matched group 2: experimental drug is adenosine cyclophosphate.
Experimental model is myocardial infarction model, causes after each group of experiment mice myocardial necrosis intravenously administrable with isoproterenol, every day twice, the dosage of each experimental drug is 5mg/kg, and administration is after 20 days, observe mice survival rate (%), result is as follows:
(1) blank group: survival rate 0;
(2) experimental group 1: survival rate 90%;
(3) experimental group 2: survival rate 90%;
(4) experimental group 3: survival rate 100%;
(5) matched group 1: survival rate 20%;
(6) matched group 2: survival rate 70%.
Can find out from above-mentioned experimental result, compare with simple use adenosine cyclophosphate or use diphenhydramine, vinpocetine and nicotinic acid combination, the compositions containing adenosine cyclophosphate of the present invention has the effect of good treatment myocardial infarction.