CN104146899A - Tomato extract as well as preparation method and application thereof - Google Patents

Abstract

The invention discloses a tomato extract as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, cleaning a tomato, crushing and homogenizing to obtain a tomato homogenate liquid; mixing the tomato homogenate liquid and water to obtain a mixed liquid; extracting the mixed liquid by ultrasonic assistance with power of 30-40w for 6-10 minutes; extracting in a water-bath kettle at 45 DEG C for 30-40 minutes after the ultrasonic-assisted extraction; centrifuging on a refrigerated centrifuge at 8000r/min and 4 DEG C for 15 minutes after the extraction is completed so as to obtain supernate; filtering the supernate, controlling the pressure of the filtrate to be 0.08-0.1MPa, and concentrating at reduced pressure and 50-65 DEG C; and controlling the temperature of the concentrated residue to be minus 20-minus 40 DEG C, and freezing and drying to obtain the tomato extract. The extract is capable of inhibiting tyrosinase, can be applied to whitening skin-care products as a tyrosinase inhibitor, is safe and reliable in performance, and does not have side effect. The preparation method is simple, and is convenient to operate.

Description

A kind of Fructus Lycopersici esculenti extract and its preparation method and application

Technical field

The invention belongs to vegetable active thing and extract field, be specifically related to a kind of Fructus Lycopersici esculenti extract and preparation method thereof and the application as tyrosinase inhibitor.

Background technology

Conventionally, the color of human skin depends primarily on melanic content and distribution, and the structure function of melanocyte and quantity directly affect melanic content in skin, is to produce melanic determiner.On skin, melanocyte is positioned at the basal layer that separates corium and epidermis.Melanocyte belongs to glandular cell, has dendrite, and it and horn cell form epidermal melanin unit.Melanin forms by a series of oxidation reactions, 3 different reactions in melanin biosynthesis approach in tyrosinase catalysis melanocyte: tyrosine (Tyr) is converted to DOPA (DOPA) under tryrosinase (TYR) catalysis, DOPA is converted into DOPA quinone (DQ) under the effect of tryrosinase, DOPA quinone autoxidation changes into dopachrome, finally generate 5,6-dihydroxy indole carboxylic acid (DHICA) to form excellent melanocyte.The latter's reaction is by carrying out under dopachrome tautomerase (Dopachrometautomerase) and the oxidasic catalysis of DHICA.Meanwhile, under the catalysis with cysteine or glutathion, DOPA quinone is converted into cysteinyl dopa or glutathion DOPA, finally generates pheomelanin.

Skin and muscles are fair is especially one of common beautiful standard of women of Aisan.People make earnest efforts pursuing whitening, use whitening product.But chemosynthesis whitening agent has larger side effect, people then pursue natural whiting agent in recent years, more existing vegetable active things have whitening function as the extract such as derivant, green tea of arbutin, vitamin C and kojic acid.These extracts belong to tyrosinase inhibitor mostly.Thereby activity that can restraint of tyrosinase reaches effect that check melanin forms.Whitening agent from resourceful plant, is extracted in exploitation and method is object of the present invention.Being of high nutritive value of Fructus Lycopersici esculenti, containing multivitamin, fruit acid, lycopene isoreactivity thing, lycopene can be removed free radical effectively, and the oxidation resistance of enhancing body has the function of slow down aging.Vitamin C has effect of whitening.Glutathion has free radical resisting and anti-oxidative stress.Fruit acid can improve the water holding capacity of cell, makes skin keep elasticity, can effectively soften epidermis cell tissue, helps surperficial keratinization cell detachment, promotes the renewal of cell, light brown patch is disappeared and take off.Fructus Lycopersici esculenti has skin whitening, antidotal effect.

Summary of the invention

The object of this invention is to provide a kind of Fructus Lycopersici esculenti extract as tyrosinase inhibitor application and preparation method thereof.

Technical scheme of the present invention

A kind of Fructus Lycopersici esculenti extract, specifically obtains by the method comprising the following steps:

(1), by Fructus Lycopersici esculenti clean, broken homogenate, obtains Fructus Lycopersici esculenti homogenate;

Fructus Lycopersici esculenti homogenate and water are calculated in mass ratio, i.e. Fructus Lycopersici esculenti homogenate: water is 1g:2-6ml, the ratio that is preferably 1g:4ml is mixed, and obtains mixed liquor;

(2), the mixed liquor of step (1) gained is extracted with ultrasound wave is auxiliary, the power of ultrasound wave assisted extraction is 60-80w, and the ultrasound wave time is 6-10min;

After ultrasonic assisted extraction in the water-bath of 45 DEG C lixiviate 30-40min, after lixiviate in 8000r/min, centrifugal 15min on the refrigerated centrifuger of 4 DEG C, the supernatant liquid filtering obtaining, collects the filtrate of gained, is Fructus Lycopersici esculenti extracting solution;

(3), be 0.08-0.1MPa by the Fructus Lycopersici esculenti extracting solution controlled pressure of step (2) gained, temperature is carried out concentrating under reduced pressure under the condition of 50-65 DEG C, the residue control temperature after concentrated is carried out lyophilization, is obtained Fructus Lycopersici esculenti extract for-20--40 DEG C.

A kind of Fructus Lycopersici esculenti extract of the present invention can be used as tyrosinase inhibitor application, due to its have remarkable inhibition-activity of tryrosinase, thereby prevent melanic formation in skin, therefore can be used as whitening agent and apply, white-skinned face function is remarkable.

Beneficial effect of the present invention

A kind of Fructus Lycopersici esculenti extract of the present invention is applied as tyrosinase inhibitor, due to its have remarkable inhibition-activity of tryrosinase, thereby prevent melanic formation in skin, therefore can be applied in skin care item, white-skinned face function is remarkable.

Further, the preparation method of a kind of Fructus Lycopersici esculenti extract of the present invention, because preparation process is only simple mixing, the step such as ultrasonic, lixiviate, filtration, concentrating under reduced pressure, lyophilization, preparation process is without the need for machine reagent, therefore its preparation process is simple, easy to operate, preparation process environmental protection, the product of gained uses as tyrosinase inhibitor, performance safety is reliable, does not have side effect.

Brief description of the drawings

Fig. 1, Fructus Lycopersici esculenti extract suppress situation to the activity of TYR enzyme;

The reducing power test of Fig. 2, Fructus Lycopersici esculenti extract.

Detailed description of the invention

Also by reference to the accompanying drawings the present invention is further set forth below by specific embodiment, but do not limit the present invention.

embodiment 1

A kind of Fructus Lycopersici esculenti extract, specifically obtains by the method preparation comprising the following steps:

(1), by Fructus Lycopersici esculenti clean, broken homogenate, obtains Fructus Lycopersici esculenti homogenate;

100g Fructus Lycopersici esculenti homogenate and 400ml water are calculated in mass ratio, i.e. Fructus Lycopersici esculenti homogenate: the ratio for 1:4 of water is mixed, and obtains mixed liquor;

(2), the mixed liquor of step (1) gained is extracted with ultrasound wave is auxiliary, the power of ultrasound wave assisted extraction is 80w, and the time is 10min;

After ultrasonic assisted extraction in the water-bath of 45 DEG C lixiviate 35min, after lixiviate in 8000r/min, centrifugal 15min on the refrigerated centrifuger of 4 DEG C, the supernatant liquid filtering obtaining, collects the filtrate of gained, is Fructus Lycopersici esculenti extracting solution;

(3), the Fructus Lycopersici esculenti of step (2) gained being extracted to filtrate is 0.08-0.1MPa through Rotary Evaporators controlled pressure, temperature is carried out concentrating under reduced pressure under the condition of 50-65 DEG C, residue control temperature after concentrated is carried out lyophilization 48h, is obtained Fructus Lycopersici esculenti extract for-20--40 DEG C.

application Example 1

the Fructus Lycopersici esculenti extract of measuring above-mentioned gained suppresses experiment to the activity of TYR enzyme

Produce dopachrome according to tyrosinase catalysis L-3,4 dihydroxyphenylalanine, dopachrome has absorption maximum at 475nm wavelength place, and its molar extinction coefficient is ε=3700 (L/molcm);

By measuring the time dependent straight line growth trend of OD475nm of enzymic catalytic reaction system, calculate enzyme activity from straight slope.

Enzyme activity unit (U) is defined as catalysis 1 μ molL-DOPA substrate per minute and is transformed into the required enzyme amount of dopachrome.

Above-mentioned measure Fructus Lycopersici esculenti extract the activity of TYR enzyme suppressed to experiment,specifically comprise the steps:

(1) preparation of the PBS buffer that, pH is 6.8

Take 8.96g disodium hydrogen phosphate and be dissolved in 500mL distilled water, obtain solution A;

Take 3g sodium hydrogen phosphate and be dissolved in 500mL distilled water, obtain solution B;

441mL solution A and 459mL solution B are mixed, obtain 900mLpH and be 6.8 PBS buffer;

(2), take 0.0049gL-DOPA, first use the dissolving with hydrochloric acid of a small amount of pH=1, after it dissolves, be settled to 50mL with the PBS buffer that the pH of above-mentioned preparation is 6.8, obtain the L-3,4 dihydroxyphenylalanine solution of 2mol/L;

(3), the PBS buffer dilution that is 6.8 by the Fructus Lycopersici esculenti extract of embodiment 1 gained with pH, obtaining respectively concentration is the Fructus Lycopersici esculenti extract solution of 127.5mg/mL, 63.75mg/mL, 51mg/mL, 31.88mg/mL, 25.5mg/mL;

(4), get respectively the PBS buffer of the pH=6.8 of 3ml step (1) gained, the concentration of 5ml step (2) gained is 2mol/L L-3,4 dihydroxyphenylalanine solution, the Fructus Lycopersici esculenti extract solution of the variable concentrations of 0.5ml step (3) gained joins in 15ml test tube, water bath with thermostatic control 10min in 25 DEG C of water-baths, finally add the tryrosinase (333U/ml) of 1.5ml (to replace tryrosinase with distilled water, as blank), 25 DEG C of reaction 3min, under 475nm, measure absorbance immediately, each concentration is done 3 Duplicate Samples, finally average, according to formula below, calculate extract pair the suppression ratio of TYR enzymatic activity.

，

Wherein A is the absorbance that does not add the blank liquid of tryrosinase;

B is the absorbance that adds tryrosinase in Fructus Lycopersici esculenti extract solution;

Taking the concentration of Fructus Lycopersici esculenti extract solution as abscissa, with the Fructus Lycopersici esculenti extract solution pair of variable concentrations the suppression ratio of TYR enzymatic activityfor vertical coordinate mapping, result as shown in Figure 1, as can be seen from Figure 1 in the time that Fructus Lycopersici esculenti extract concentration is 25.5-52.5mg/mL, along with the increase of Fructus Lycopersici esculenti extract concentration, suppression ratio increases rapidly, and in the time that Fructus Lycopersici esculenti extract concentration is 52.5-127.5mg/mL, the speed that suppression ratio increases slows down, particularly the suppression ratio of Fructus Lycopersici esculenti extract in the time that concentration is 127.5mg/mL is 99.31%, substantially completely the activity of restraint of tyrosinase.Show thus, Fructus Lycopersici esculenti extract of the present invention is applied as tyrosinase inhibitor.

Calculate the concentration of the test sample that suppresses 50% tyrosinase activity under experiment condition.Be that suppression ratio carries out linear fit to inhibitor concentration, draw IC50, its IC50 is 46.64mg/mL, has shown that thus tomato juice extract is very significantly whitening agent of a kind of effect.

application Example 2

the test of the reducing power of Fructus Lycopersici esculenti extract, concrete steps are as follows:

First, join in the Fructus Lycopersici esculenti extract of embodiment 1 gained with the pH=6.8 phosphate buffer solution of gained in Application Example 1, obtain that concentration is respectively 16.24,10.15,8.12,5.075, the Fructus Lycopersici esculenti extract solution of 4.06mg/mL;

Then, in the Fructus Lycopersici esculenti extract solution of each variable concentrations, adding respectively 1ml mass percent concentration is 1% potassium ferricyanide aqueous solution, then controlling temperature is to be incubated after 20min under 50 DEG C of conditions, be cooled to rapidly 25 DEG C, add respectively again 2.5ml mass percent concentration and be 10% trichloroacetic acid solution, in 3500r/min centrifugalize 10min, then get respectively supernatant 2.5ml, add respectively again that 2.5ml is two heats up in a steamer water, then to add respectively 0.5ml mass percent concentration be 0.1% FeCl ₃aqueous solution, after mix homogeneously, under wavelength 700nm, survey absorbance, taking the concentration of Fructus Lycopersici esculenti extract solution as abscissa, the mapping taking the absorbance of Fructus Lycopersici esculenti extract solution as vertical coordinate, result as shown in Figure 2, as can be seen from Figure 2 in the time that tomato juice extract concentration is 4.06-16.24mg/mL, along with the growth of tomato juice extract concentration, absorbance is linear growth trend.Show thus, the oxidation resistance of Fructus Lycopersici esculenti extract is along with the concentration of Fructus Lycopersici esculenti extract increases, and absorbance increases, and oxidation resistance strengthens.

In sum, a kind of Fructus Lycopersici esculenti extract provided by the invention, has the activity of good restraint of tyrosinase, also has oxidation resistance clearly, therefore can be used as skin-whitening agents and uses.

The above is only giving an example of embodiments of the present invention; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.

Claims (3)

1. a Fructus Lycopersici esculenti extract, is characterized in that described Fructus Lycopersici esculenti extract specifically obtains by the method comprising the following steps:

(1), by Fructus Lycopersici esculenti clean, broken homogenate, obtains Fructus Lycopersici esculenti homogenate;

Fructus Lycopersici esculenti homogenate and water are calculated in mass ratio, i.e. Fructus Lycopersici esculenti homogenate: the ratio that water is 1g:2-6ml is mixed, and obtains mixed liquor;

(2), the mixed liquor of step (1) gained is extracted with ultrasound wave is auxiliary, the power of ultrasound wave assisted extraction is 60-80w, and the time is 6-10min;

After ultrasonic assisted extraction in the water-bath of 45 DEG C lixiviate 30-40min, after lixiviate in 8000r/min, centrifugal 15min on the refrigerated centrifuger of 4 DEG C, the supernatant liquid filtering obtaining, collects the filtrate of gained, is Fructus Lycopersici esculenti extracting solution;

(3), be 0.08-0.1MPa by the Fructus Lycopersici esculenti extracting solution controlled pressure of step (2) gained, temperature is carried out concentrating under reduced pressure under the condition of 50-65 DEG C, the residue control temperature after concentrated is carried out lyophilization, is obtained Fructus Lycopersici esculenti extract for-20--40 DEG C.

2. a kind of Fructus Lycopersici esculenti extract as claimed in claim 1, is characterized in that Fructus Lycopersici esculenti homogenate in preparation process (1): water is 1g:4ml.

3. Fructus Lycopersici esculenti extract as claimed in claim 1 or 2 is applied as tyrosinase inhibitor.