CN104137833B - One is prevented and treated the biological composite bactericide of wheat scab and application thereof - Google Patents
One is prevented and treated the biological composite bactericide of wheat scab and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of biological composite bactericide of preventing and treating wheat scab, described biological composite bactericide comprises jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate, and the weight proportion scope of described jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate is 1:20～20:1. The invention also discloses the above-mentioned biological composite bactericide of preventing and treating wheat scab in the application preventing and treating aspect wheat scab. In compound drug of the present invention, jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate are respectively the bactericide of the different mechanisms of action, and the past prevents and treats between the main medicament carbendazim of wheat scab and triazolone without the mutual resistance to the action of a drug; Wide, the interior absorption of said preparation fungicidal spectrum is strong, and quick-acting is good, the lasting period is long, environmentally friendly and have cost low and processing easy advantage.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to one and prevent and treat the biological composite bactericide of wheat scab and shouldWith.
Background technology
Wheat scab is a kind of fulminant, destructive disease, while generation greatly, can cause wheat Severe Reduction, even total crop failure,And sick wheat contains toxin, after eating, can cause person poultry poisoning, within nearly 2 years, occur heavier. Mainly infect small ear, whole when seriousThe individual tassel later stage is all withered, and fringe portion produces the mould layer of pink colloid, seed shrinkage, shrivelled. This kind of disease is from wheat seedlingPhase to the middle and later periods can be infected harm, especially attaches most importance to the morbidity of wheat heading flowering period. Generally can the underproduction 1～2Become, retransmitting the sick time can the underproduction 5～6 one-tenths, and even total crop failure, also can cause in people and animals after the wheat of falling ill is eaten by people and animalsPoison, constitutes a serious threat to Wheat Production and human and livestock health. Therefore, fully grasp wheat scab occurrence regularity, choosingPunctual machine, optimum period prevention, most important to High-yield Wheat good harvest. In recent years, Yangtze river basin overwhelming majority gibberella saubinetiiDisease is situation occurred frequently. Cause the large generation of gibberella saubinetii disease popular main cause to have two: the one, weather conditions adapt toIn the popular propagation of pathogen; The 2nd, fusarium graminearum is to preventing and treating the anti-medicine of main medicament carbendazim (carbendazim)Property level and resistant strain ratio are high, and carbendazim and the complex preparation taking carbendazim as main composition (many ketone) are in positive usual amounts(level) is lower can not this disease of effectively preventing, particularly red mould toxin can not effectively be controlled, and prevents and treats unsuccessfully to subregion.Current, worldwide also there is no the wheat breed of high resistance wheat scab, so chemical control is still main improvementMeasure. Jiangsu Prov. Inst. of Agricultural Chemicals develops the efficient new medicament of 2-cyano-3-amino-3-phenylancryic acetate, obtains preferably preventing and treating in wheat scabApplication, but because 2-cyano-3-amino-3-phenylancryic acetate easily develops immunity to drugs, produce for delaying the resistance to the action of a drug, be badly in need of screening (or composite) and go out and can haveThis sick novel built bactericide of effect control.
Summary of the invention
Goal of the invention: first object of the present invention be to prevent with delay wheat scab to new and effective medicament--cyanogenThe resistance to the action of a drug of alkene bacterium ester, improves and prevents and treats wheat scab effect, provides a kind of wheat scab is had to high prevention effectJinggangmycin A and the biological composite bactericide of 2-cyano-3-amino-3-phenylancryic acetate. Another object of the present invention has been to provide that above-mentioned biology is composite to killThe application of microbial inoculum. The invention provides the biological complex preparation of jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate. This complex preparation and current anti-Control wheat scab main medicament carbendazim and and triazole type between without the mutual resistance to the action of a drug, can effectively administer wheat scabThe resistance to the action of a drug of bacterium to carbendazim and triazole type, and prevent and continue that domestic initiative new varieties--2-cyano-3-amino-3-phenylancryic acetate is to wheat scabBacterium action site is single, problem easily develops immunity to drugs. Invent the best synergy ratio range providing between two medicaments with multiplePreparaton processing and application thereof. Result of the test shows, complex preparation is remarkable to the synergy of wheat scab compared with single dose,Field control effect is higher than single dose, and cost is also lower than 2-cyano-3-amino-3-phenylancryic acetate single dose.
Technical scheme: for solving the problems of the technologies described above, the invention provides a kind of composite killing of biology of preventing and treating wheat scabMicrobial inoculum, described biological composite bactericide comprises jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate, described jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetateWeight proportion scope be 1:20～20:1.
Wherein, above-mentioned jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate weight ratio 1:1～9.
Wherein, above-mentioned jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate weight ratio 1:5.
Wherein, above-mentioned biological composite bactericide also comprises auxiliary agent and inserts.
Wherein, above-mentioned biological composite bactericide is the one in wettable powder, suspending agent, microemulsion or water dispersible granulesOr several.
Wherein, in the time that above-mentioned biological composite bactericide is wettable powder, described biological composite bactericide by weight byFollowing raw material composition: jinggangmycin A 1～20%, 2-cyano-3-amino-3-phenylancryic acetate 1～20%, auxiliary agent 10～15%, surplus are inserts.
Wherein, in the time that above-mentioned biological composite bactericide is wettable powder, described biological composite bactericide by weight byFollowing raw material composition: jinggangmycin A 1～20%, 2-cyano-3-amino-3-phenylancryic acetate 1～50%, auxiliary agent 10～15%, surplus are inserts.
Wherein, when above-mentioned auxiliary agent is lauryl sodium sulfate, ethoxylated dodecyl alcohol, lignosulfonates, sulfonic acid gatherFormaldehyde condensation products, polyethylene glycol, ammonium sulfate, sodium carboxymethylcellulose, sodium butylnaphthalenesulfonate, 1-METHYLPYRROLIDONE,Alkyl benzene calcium sulfonate, phenethyl phenol mela mine-formaldehyde resin polyoxy ethylether, ethylene glycol, silicone compound, xanthans, silicic acidOne or more in magnalium.
Wherein, when above-mentioned inserts is one or more in kaolin, diatomite, precipitated calcium carbonate or aluminium-magnesium silicate.
The above-mentioned biological composite bactericide of preventing and treating wheat scab is in the application preventing and treating aspect wheat scab.
Jinggangmycin A of the present invention and 2-cyano-3-amino-3-phenylancryic acetate composite bactericide are wheat in the application optimum period of preventing and treating wheat scabBlooming stage, be watered 600kg carefully spray control 1-2 time.
Beneficial effect: compounded combination bactericide of the present invention, compared with conventional sterilization agent, has the following advantages: the present invention is multipleIn dose out powders, jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate are respectively the bactericide of the different mechanisms of action, prevent and treat wheat scab with the pastMain medicament carbendazim and triazolone between without the mutual resistance to the action of a drug; Wide, the interior absorption of said preparation fungicidal spectrum is strong, quick-acting is good,Lasting period is long, environmentally friendly and have cost low and processing easy advantage.
Detailed description of the invention
Embodiment 1:24% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Jinggangmycin A 4%, 2-cyano-3-amino-3-phenylancryic acetate 20%, NNO8%, K12 (lauryl sodium sulfate) 4%, all the other areInserts kaolin.
Said components is mixed, and through air-flow crushing, to 350-400 order fineness, it is mould that analytical control warehouse-in obtains 24% well ridgeElement A 2-cyano-3-amino-3-phenylancryic acetate wettable powder.
Embodiment 2:40% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate suspending agent
Jinggangmycin A 20%, 2-cyano-3-amino-3-phenylancryic acetate 20%, lauryl sodium sulfate 2%, Magnesiumaluminumsilicate 5%, xanthans 3%,Ethylene glycol 5%, water 45%.
By said components mix, after high speed shear and sand mill grinds, analytical control warehouse-in obtains 40% jinggangmycin A cyanogenAlkene bacterium ester suspending agent.
Embodiment 316% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate microemulsion
Jinggangmycin A 8%, 2-cyano-3-amino-3-phenylancryic acetate 8%, 1-METHYLPYRROLIDONE 1%, alkyl benzene calcium sulfonate 2%, phenethyl phenolMela mine-formaldehyde resin polyoxy ethylether 2%, ethylene glycol 1%, silicone compound 1%, xanthans 2%, aluminium-magnesium silicate 1%,Water 74%, makes 16% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate microemulsion.
By jinggangmycin A, 2-cyano-3-amino-3-phenylancryic acetate, solvent 1-METHYLPYRROLIDONE, emulsifying agent alkyl benzene calcium sulfonate and phenethyl phenolMela mine-formaldehyde resin polyoxy ethylether is added together, and is dissolved into even oil phase; By water, antifreeze ethylene glycol, thickener xanthans,Defoamer silicone compound mixes, and becomes homogeneous water. Under high-speed stirred, water is added to oil phase,Make 16% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate microemulsion.
Embodiment 4:20% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules
Jinggangmycin A 10%, 2-cyano-3-amino-3-phenylancryic acetate 10%, neopelex 10%, urea 3%, precipitated calcium carbonate 67%.
Said components is mixed, through pulverizing the female powder of preparation, female powder is mixed with suitable quantity of water solution, high speed shear alsoSand mill grinds, and then carries out fluidized bed prilling, and dry, screening, analytical control warehouse-in obtain 20% jinggangmycin A cyanogenThe water dispersible granules of alkene bacterium ester.
Embodiment 5:15% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Jinggangmycin A 5%, 2-cyano-3-amino-3-phenylancryic acetate 10%, NNO8%, K12 (lauryl sodium sulfate) 4%, all the other areInserts kaolin.
Said components is mixed, and through air-flow crushing, to 350-400 order fineness, it is mould that analytical control warehouse-in obtains 15% well ridgeElement A 2-cyano-3-amino-3-phenylancryic acetate wettable powder.
Embodiment 6:20% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Jinggangmycin A 5%, 2-cyano-3-amino-3-phenylancryic acetate 15%, NNO8%, K12 (lauryl sodium sulfate) 4%, all the other areInserts kaolin.
Said components is mixed, and through air-flow crushing, to 350-400 order fineness, it is mould that analytical control warehouse-in obtains 20% well ridgeElement A 2-cyano-3-amino-3-phenylancryic acetate wettable powder.
Embodiment 716% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Jinggangmycin A 2%, 2-cyano-3-amino-3-phenylancryic acetate 14%, NNO8%, K12 (lauryl sodium sulfate) 4%, all the other areInserts kaolin.
Said components is mixed, and through air-flow crushing, to 350-400 order fineness, it is mould that analytical control warehouse-in obtains 16% well ridgeElement A 2-cyano-3-amino-3-phenylancryic acetate wettable powder.
Embodiment 820% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder
Jinggangmycin A 2%, 2-cyano-3-amino-3-phenylancryic acetate 18%, NNO8%, K12 (lauryl sodium sulfate) 4%, all the other areInserts kaolin.
Said components is mixed, and through air-flow crushing, to 350-400 order fineness, it is mould that analytical control warehouse-in obtains 20% well ridgeElement A 2-cyano-3-amino-3-phenylancryic acetate wettable powder.
Experimental example:
Jinggangmycin A of the present invention and 2-cyano-3-amino-3-phenylancryic acetate composite bactericide are determined as follows the toxicity of fusarium graminearum:
The female medicine of jinggangmycin A and the former medicinal acetone of 2-cyano-3-amino-3-phenylancryic acetate are dissolved into respectively 1000 μ g/mL mother liquors, and add 1% to tellTemperature-80. Be mixed with concentration and be respectively the single dose of 200,100,20,10,2,1,0.2 and 0.1 μ g/mL with sterilized waterSeries liquid, is placed in triangular flask for subsequent use. By Prochloraz and own azoles alcohol by effective content prepare respectively become 1:1,1:3,The serial test solution of 1:5,1:7 and 1:9 mixture, is placed in triangular flask for subsequent use. Get respectively above-mentioned serial dilution medicament 1mLLiquid and 9mL culture medium (PDA) mix in culture dish, make in culture dish content of dispersion and be 20,10,2,1,0.2, the PDA culture medium of 0.1,0.02 and 0.01 μ g/mL, makes blank with sterilized water. By the gibberella saubinetii of preservingGerm is transferred in PDA plate, activated after, produce with card punch the bacterium cake that diameter is 4mm at nearly colony edge,Be transferred in the PDA plate and blank with medicine, every processing repeats 4 times again. The dark 4d that cultivates at 25 DEG C.
When bacterium colony covers with plate soon in blank, adopt right-angled intersection method to measure colony diameter. Calculating colony diameter is flatAverage, and calculate according to the following formula the average inhibiting rate of mycelial growth: the average inhibiting rate of mycelial growth=[(contrast colony diameterAverage-processing colony diameter average)/(contrast colony diameter average-inoculation bacterium cake diameter)] × 100%. Adopt DPS data placeReason system, calculates regression equation, EC50With EC90And 95% confidence limit. Press Wadley method, calculate synergy systemNumber (SR).
The synergy type of evaluating pharmacy mix according to coefficient of synergism (SR), SR < 0.5 is antagonism,0.5≤SR≤1.5 are summation action, SR > 1.5 for synergistic effect. SR=EC50(Eth)/EC50(Eob)，EC50(Eth)＝(a+b)/[(a/EC50A)+(b/EC50B)]. Wherein, A, B are respectively bactericide single dose, and a, b are that corresponding single dose existsRatio in mixture, EC50(Eth) be mixture EC50Theoretical value, EC50(Eob) be mixture EC50Measured value.
Complex preparation and single dose show the Toxicity Determination result of fusarium graminearum, jinggangmycin A, 2-cyano-3-amino-3-phenylancryic acetateTo the EC of fusarium graminearum50Be respectively 95.4673 μ g/mL and 0.1208 μ g/mL, 2-cyano-3-amino-3-phenylancryic acetate is to gibberella saubinetiiThe indoor biologically active of germ high (table 1).
It is red to wheat that jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate are pressed respectively 1:1,1:3,1:5,1:7 and the combination of 1:9 equal proportion mixtureThe EC of mould germ50Be respectively 0.1430 μ g/mL, 0.0969 μ g/mL, 0.0758 μ g/mL, 0.0815 μ g/mL and0.0938 μ g/mL, 5 kinds of mixtures combine coefficient of synergism (SR) to fusarium graminearum be respectively 1.69,1.66,1.91,1.69 and 1.43. Jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate are the highest to the synergistic effect of fusarium graminearum in the ratio mixture of 1:5,Reach extremely significantly (coefficient of synergism SR reaches 1.91).
Table 1 jinggangmycin A, 2-cyano-3-amino-3-phenylancryic acetate and the built agent inhibitory action to the growth of wheat scab mycelia
Application Example
Bactericidal agent for preventing and treating wheat scab of the present invention
The field controling test of the bactericide of employing embodiment 1～8 to wheat scab. Spraying time is the wheat flowering initial stagePrevent and treat 1 time. Wheat breed is town wheat 168, and water consumption is 600kg/hm2, the method for preventing and treating is for thin spraying.
Experimental concentration is designed to:
Process (1) embodiment 1: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder 270g.ai/hm2；
Process (2) embodiment 2: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate suspending agent 270g.ai/hm2；
Process (3) embodiment 3: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate microemulsion 270g.ai/hm2；
Process (4) embodiment 4: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate water dispersible granules 270g.ai/hm2；
Process (5) embodiment 5: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder 270g.ai/hm2；
Process (6) embodiment 6: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder 270g.ai/hm2；
Process (7) embodiment 7: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder 270g.ai/hm2；
Process (8) embodiment 8: jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate wettable powder 270g.ai/hm2；
Process (9) 50% carbendazol wettable powder 600g.ai/hm2；
Process (10) 10% jinggangmeisu aqua 360g.ai/hm2；
Process (11) 25% 2-cyano-3-amino-3-phenylancryic acetate suspending agent 375g.ai/hm2；
Process (12) clear water.
Field control result shows, each processing in the situation that of wheat flowering initial stage control 1 time, each embodiment 1～8, withAnd the control of 50% carbendazol wettable powder, 10% jinggangmycin A water solube powder and 25% 2-cyano-3-amino-3-phenylancryic acetate suspending agentEffect is respectively 92.31%, 87.69%, 86.77%, 86.64%, 91.14%,, 94.12%, 91.70%, 91.28%,80.25%, 68.88% and 84.96% (table 2).
The field control result of table 224% jinggangmycin A 2-cyano-3-amino-3-phenylancryic acetate to wheat scab
Result of the test shows: after jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate are composite, and, compared with each unitary agent dosage wherein, everyHectare has reduced effective ingredient consumption greatlyr, and a dispenser preventive effect is improved, wherein embodiment 1, embodiment 5,Embodiment 6, embodiment 7, embodiment 8 preventive effects all exceed 90%, and synergy is obvious. And anti-with conventional pharmacopoeia carbendazimEffect is also improved. Reduce dosage and use cost, have good economy, society and ecological benefits.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art,Under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered asProtection scope of the present invention.
Claims (9)
1. a biological composite bactericide of preventing and treating wheat scab, is characterized in that, described biological composite bactericide comprises jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate, described jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate weight ratio 1:1 ~ 9.
2. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 1, is characterized in that, described jinggangmycin A and 2-cyano-3-amino-3-phenylancryic acetate weight ratio 1:5.
3. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 1, is characterized in that, described biological composite bactericide also comprises auxiliary agent and inserts.
4. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 1, is characterized in that, described biological composite bactericide is the one in wettable powder, suspending agent, microemulsion or water dispersible granules.
5. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 4, it is characterized in that, in the time that described biological composite bactericide is wettable powder, described biological composite bactericide is made up of following raw material by weight: jinggangmycin A 1～20%, 2-cyano-3-amino-3-phenylancryic acetate 1～20%, auxiliary agent 10～15%, surplus are inserts.
6. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 5, it is characterized in that, in the time that described biological composite bactericide is wettable powder, described biological composite bactericide is made up of following raw material by weight: jinggangmycin A 5～20%, 2-cyano-3-amino-3-phenylancryic acetate 10～20%, auxiliary agent 10～15%, surplus are inserts.
7. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 3, it is characterized in that, described auxiliary agent is lauryl sodium sulfate, ethoxylated dodecyl alcohol, one or more in lignosulfonates, sulfonic acid polyformaldehyde condensation product, polyethylene glycol, ammonium sulfate, sodium carboxymethylcellulose, sodium butylnaphthalenesulfonate, 1-METHYLPYRROLIDONE, alkyl benzene calcium sulfonate, phenethyl phenol mela mine-formaldehyde resin polyoxy ethylether, ethylene glycol, silicone compound, xanthans, aluminium-magnesium silicate.
8. a kind of biological composite bactericide of preventing and treating wheat scab according to claim 3, is characterized in that, described inserts is one or more in kaolin, diatomite, precipitated calcium carbonate or aluminium-magnesium silicate.
9. the biological composite bactericide of preventing and treating wheat scab described in claim 1 ~ 8 any one is in the application preventing and treating aspect wheat scab.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105766937B (en) * | 2016-05-10 | 2018-03-30 | 南京农业大学 | The bactericidal composition and its application of a kind of jinggangmeisu and kind bacterium azoles |
| CN106719807A (en) * | 2016-11-30 | 2017-05-31 | 谢松芬 | The compound drug of wheat scab |
| CN106973899A (en) * | 2017-04-12 | 2017-07-25 | 江苏金凤凰农化有限公司 | A kind of composition and application process that wheat scab is treated based on guaiacol |
| CN107494538A (en) * | 2017-10-17 | 2017-12-22 | 惠州市无龄康态健康科技有限公司 | Argentiferous fruit and the composition pesticide of 2-cyano-3-amino-3-phenylancryic acetate |
| CN109907048A (en) * | 2017-12-12 | 2019-06-21 | 株洲千金药业股份有限公司 | Application of the propanoic derivatives in preparation prevention and treatment gibberella saubinetii medicine |
| CN108849923A (en) * | 2018-08-07 | 2018-11-23 | 安徽丰乐农化有限责任公司 | A kind of biological composite bactericide for preventing and treating wheat scab |
| CN109496738A (en) * | 2018-11-13 | 2019-03-22 | 江西农业大学 | White lotus pest control method |
| CN109644996B (en) * | 2018-12-10 | 2021-11-16 | 上海惠光环境科技有限公司 | Suspending agent composition containing validamycin A and hexaconazole |
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