CN104137765A - Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line - Google Patents
Breeding method of high-stress resistance elongated upper internode two-line genic male sterile line Download PDFInfo
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Abstract
The invention discloses a breeding method of a high-stress resistance elongated upper internode two-line genic male sterile line and belongs to the technical field of the rice breeding for stress resistance. The method is characterized in that a drought-resistant variety Lvhan No.1, a hardy variety Dongye No.1 and a saline-alkaline tolerance variety Dongdao No.4 are taken as resistant resource parents and elongated upper internode two-line genic male sterile lines Fu eS1 and Guangzhan 63eS are taken as recurrent parents, the resistant source genes of the three rice resistance properties are pyramided into an elongated upper internode two-line genic male sterile line material by use of a cross-backcross pyramiding method, and consequently, the elongated upper internode two-line genic male sterile line which is highly consistent with the recurrent parent of the elongated upper internode two-line genic male sterile line in agronomic traits, sterile period conversion temperatures and sterility and has high resistance can be bred through mufti-generation backcross and screening; and the high-resistance elongated upper internode two-line genic male sterile line is used for producing two-line hybrid rice and the resistance level of the hybrid rice can be increased.
Description
Technical field
The present invention is specifically related to the selection of the long fringe neck of a kind of high resistance to cold and diseases two-line sterile line, belongs to paddy rice anti contravariance breeding technical field.
Background technology
Paddy rice, as the first cereal crops of China, makes a great contribution to solving Food Security in China and improving people ' s health level.Paddy rice is called paddy rice anti contravariance to various coercing (adverse circumstance) factor as the resist ability of cold-resistant, drought resisting, anti-salt etc.In recent years, Global Natural Disasters frequency is many, trend and agricultural workforce's shortage etc. of population increase, shortage of water resources, soil alkaline desertification simultaneously increasingly sharpens, that brings thus variously coerces the production that directly has influence on paddy rice, saline and alkaline, arid, low temperature etc. become the main cause of Rice Yield Loss Caused, in recent years breeding man resistance breeding mechanism and resistance breeding have been made to large quantity research, and have made some progress, also be bred as some resistance kinds, but be mostly single resistance kind.Cultivate the energy high yielding variety that the drought-resistant lower temperature resistance of Salt And Alkali Tolerance is strong comprehensively for breaking through the predicament in short supply of current China's resistance kind, the severe times of ecotope such as reply arid, saline land, for Food Security in China increases new technological reserve, ensure that national food has important practical significance safely.The two-line sterile line of hybrid rice exists the defect of inheritance Bao Sui, seed is produced must use " 920 " (gibberellin), not only increase seed production cost, and use " 920 " and also caused environmental pollution, University Of Agriculture and Forestry In Fujian utilizes the long fringe neck two-line sterile line of eui gene seed selection can reach heredity releasing or alleviate Bao Sui, use no or little " 920 ", thereby the seed production cost of hybrid rice is declined, seed quality improves and reduces environmental pollution.But equally also there is the poor problem of resistance in long fringe neck two-line sterile line.
summary of the invention
The object of the present invention is to provide the selection of the long fringe neck of a kind of high resistance to cold and diseases two-line sterile line, thereby greatly improve the resistance level of long fringe neck two-line sterile line, the resistance level that can improve hybrid rice with its preparation two-line hybrid rice is significant to guaranteeing Rice Production and grain security.
For reaching above object, the present invention by the following technical solutions:
A selection for the long fringe neck of high resistance to cold and diseases two-line sterile line, comprises the following steps:
1) select drought-resistant variety-A, cold-resistant variety-B, Salt And Alkali Tolerance kind-C, long fringe neck two-line sterile line recurrent parent S-D, make respectively hybridization A/B and D/C, must hybridize F
1in generation,, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, F must reestablish diplomatic relations
1, with F
1-ABCDrepresent;
3) plantation F
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1500-1800 strain, carries out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance, select to have the rice strain listing mark of long fringe neck proterties flowering stage, maturing stage is individual plant sowing respectively, obtains F
3-ABCD;
4) strain plantation F
3-ABCD, recurrent parent S is planted in each strain plantation 40-50 strain simultaneously, selects individual plant and the recurrent parent S hybridization of economical character excellence flowering stage, and recurrent parent S does maternal, must hybridize F
1in generation,, with SF
1-ABCDrepresent;
5) plantation 4) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, plant recurrent parent S simultaneously, seedling stage is to SF
2-ABCDcarry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance;
6) select the SF of economical character excellence flowering stage
2-ABCDindividual plant and recurrent parent S backcross, and recurrent parent S does maternal, obtains 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, plant recurrent parent S simultaneously, seedling stage is to BC
1f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, and the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, selects the individual plant of listing of economical character excellence to continue to backcross with recurrent parent S flowering stage, and recurrent parent S makes female parent, and BC must backcross
2f
1generation;
7) plantation BC
2f
1for seed, selfing obtains BC
2f
2generation, plantation BC
2f
2generation, plant recurrent parent S simultaneously, seedling stage is to BC
2f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, select economical character excellence,, pollen abortion thoroughly individual plant listing mark the most similar to recurrent parent S flowering stage, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket regeneration, observe pollen fertility flowering stage, select the individual plant sowing that pollen fertility is good, ripening rate is high;
8) strain plantation 7) gained individual plant, its economical character, pollen fertility, fertility conversion temperature are investigated to screening, selection economical character is similar to recurrent parent S, the phase of can educating shows long fringe neck proterties, sterile phase pollen abortion is thorough and the fertility conversion temperature single-plant propagation seed close with recurrent parent S, is bred as the long fringe neck of high resistance to cold and diseases two-line sterile line.
Described long fringe neck two-line sterile line recurrent parent S is good fortune eS1 or extensively accounts for 63eS.
Described step 5) is to step 8), every step that needs hybridization or sowing, all being arranged in two-line sterile line the flowering stage of its material plantation can educate heading under temperature condition and bloom, the every step that need to identify the pollen fertility of material is all arranged under two-line sterile line fertility conversion lower limit temperature condition ears and blooms, and the procedure that the fertility conversion temperature of material is identified carries out in climatic cabinate.
Wherein drought-resistant variety is green non-irrigated No. 1, and cold-resistant variety is wild No. of east, and Salt And Alkali Tolerance kind is eastern rice No. four.
beneficial effect of the present invention:
Effect of the present invention is respectively controlling drought resisting, cold-resistant, the anti-source gene of Salt And Alkali Tolerance imports in long fringe neck two-line sterile line by hybridization and back cross breeding method, be bred as identical with former two-line sterile line genetic background but there is the long fringe neck two-line sterile line of high resistance to cold and diseases, the long fringe neck two-line sterile line agronomy of high resistance to cold and diseases is highly consistent with former male sterile line parent with yield traits, still possesses high yield, high-quality characteristic, but the two-line hybrid rice good stress resistance of the long fringe neck two-line sterile line preparation of high resistance to cold and diseases, can adapt to arid, cold damage, the harsh weather such as saline land and soil condition, ensure output, under harsh weather and soil condition, every mu can increase output 15-25% than check variety.
Embodiment
Embodiment 1:
1) select drought-resistant variety green non-irrigated No. 1 (A) (authorization numbering: state examines rice 2005053; Country's variety of crops power protection; notification number: CNA002893E), cold-resistant variety east wild No. one (B) (Chen great Zhou. Dongxiang Wild Rice cold resistance research and apply ［ D ］. Nanjing: Agricultural University Of Nanjing; 2004), No. four (C) (authorization numberings: lucky careful rice 2010005) of Salt And Alkali Tolerance kind east rice, long fringe neck two-line sterile line good fortune eS1(D); make respectively hybridization A/B and D/C, must hybridize F
1in generation,, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, F must reestablish diplomatic relations
1, with F
1-ABCDrepresent;
3) plantation F
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1500-1800 strain, carries out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance, select to have the rice strain listing mark of long fringe neck proterties flowering stage, maturing stage is individual plant sowing respectively, obtains F
3-ABCD;
4) strain is planted the F that has long fringe neck proterties and entirely resist 3 kinds of adverse circumstances
3-ABCDsingle-strain seed, good fortune eS1 is planted in each strain plantation 40-50 strain simultaneously, selects flowering stage 5 of the individual plants of economical character excellence to do maternal with good fortune eS1() hybridization, obtain 5 hybridization F
1in generation,, with SF
1-ABCDrepresent;
5) plantation 4) 5 SF of gained
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, plant good fortune eS1 simultaneously, seedling stage is to SF
2-ABCDcarry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance;
6) select the SF of economical character excellence flowering stage
2-ABCD5 of individual plants are done maternal with good fortune eS1() backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, plant good fortune eS1 simultaneously, seedling stage is to BC
1f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, and the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, selects 5 of the individual plants of listing of economical character excellence to do maternal with good fortune eS1(flowering stage) continue to backcross, obtain 5 BC that backcross
2f
1generation;
7) plantation BC
2f
1for seed, selfing obtains BC
2f
2generation, plantation BC
2f
2generation, plant good fortune eS1 simultaneously, seedling stage is to BC
2f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, select economical character excellence,, pollen sterility thoroughly individual plant 30 strain listing marks the most similar to good fortune eS1 flowering stage, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket regeneration, observe pollen fertility flowering stage, select 10 sowings of individual plant that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, its economical character, pollen fertility, sterile conversion temperature are investigated to screening, select the single-plant propagation seed that economical character is similar to good fortune eS1, the phase of can educating shows long fringe neck proterties, the thorough and sterile conversion temperature of sterile phase pollen sterility is close with good fortune eS1 to be bred as the long fringe neck of high resistance to cold and diseases two-line sterile line " degeneration-resistant good fortune eS1 ".
Step 5) is to step 8), every step that needs hybridization or sowing, all being arranged in two-line sterile line the flowering stage of its material plantation can educate heading under temperature condition and bloom, the every step that need to identify the pollen fertility of material is all arranged under two-line sterile line fertility conversion lower limit temperature condition ears and blooms, and the procedure that the fertility conversion temperature of material is identified carries out in climatic cabinate.
" the degeneration-resistant good fortune eS1 " of seed selection of the present invention possesses high yield, high-quality characteristic, two-line hybrid rice " degeneration-resistant good fortune eS1 "/9311 good stress resistance that " degeneration-resistant good fortune eS1 " and restorer 9311 are prepared, can adapt to harsh weather and the soil conditions such as arid, cold damage, saline land, ensured output, under harsh weather and soil condition, every mu increases output 15-25% than check variety good fortune eS1/9311.
Embodiment 2:
1) select drought-resistant variety green non-irrigated No. 1 (A), cold-resistant variety east wild No. one (B), Salt And Alkali Tolerance kind east rice No. four (C), long fringe neck two-line sterile lines extensively to account for 63eS(D), make respectively hybridization A/B and D/C, must hybridize F
1in generation,, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, F must reestablish diplomatic relations
1, with F
1-ABCDrepresent;
3) plantation F
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1500-1800 strain, carries out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance, select to have the rice strain listing mark of long fringe neck proterties flowering stage, maturing stage is individual plant sowing respectively, obtains F
3-ABCD;
4) strain is planted the F that has long fringe neck proterties and entirely resist 3 kinds of adverse circumstances
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plantation simultaneously extensively accounts for 63eS, selects 5 of the individual plants of economical character excellence flowering stage and extensively account for 63eS(to do maternal) hybridization, obtain 5 hybridization F
1in generation,, with SF
1-ABCDrepresent;
5) plantation 4) 5 SF of gained
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, plantation simultaneously extensively accounts for 63eS, and seedling stage is to SF
2-ABCDcarry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance;
6) select the SF of economical character excellence flowering stage
2-ABCD5 of individual plants with extensively account for 63eS(and do maternal) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, plant good fortune eS1 simultaneously, seedling stage is to BC
1f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, select flowering stage economical character excellence list 5 of individual plants with extensively account for 63eS(and do maternal) continue to backcross, obtain 5 BC that backcross
2f
1generation;
7) plantation BC
2f
1for seed, selfing obtains BC
2f
2generation, plantation BC
2f
2in generation, plantation simultaneously extensively accounts for 63eS, and seedling stage is to BC
2f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, select flowering stage economical character excellence, to extensively account for that 63eS is the most similar, pollen sterility individual plant 30 strain listing marks thoroughly, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket regeneration, observe pollen fertility flowering stage, select 10 sowings of individual plant that pollen fertility is good, ripening rate is high;
8) strain plantation 7) selected 10 individual plants, its economical character, pollen fertility, fertility conversion temperature are investigated to screening, select economical character with extensively account for 63eS similar, can educate the thorough and fertility conversion temperature of the long fringe neck proterties of phase performance, sterile phase pollen sterility and extensively account for the single-plant propagation seed that 63eS is close and be bred as the long fringe neck of high resistance to cold and diseases two-line sterile line " the degeneration-resistant 63eS that extensively accounts for ".
Step 5) is to step 8), every step that needs hybridization or sowing, all being arranged in two-line sterile line the flowering stage of its material plantation can educate heading under temperature condition and bloom, the every step that need to identify the pollen fertility of material is all arranged under two-line sterile line fertility conversion lower limit temperature condition ears and blooms, and the procedure that the fertility conversion temperature of material is identified carries out in climatic cabinate.
" the degeneration-resistant 63eS that extensively accounts for " of seed selection of the present invention possesses high yield, high-quality characteristic, two-line hybrid rice " the degeneration-resistant 63eS that extensively accounts for "/9311 good stress resistance that " the degeneration-resistant 63eS that extensively accounts for " and restorer 9311 are prepared, can adapt to harsh weather and the soil conditions such as arid, cold damage, saline land, ensured output, under harsh weather and soil condition, every mu increases output 18-25% than the wide 63eS/9311 that accounts for of check variety.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. the selection of the long fringe neck of high resistance to cold and diseases two-line sterile line, is characterized in that: described selection comprises the following steps:
1) select drought-resistant variety-A, cold-resistant variety-B, Salt And Alkali Tolerance kind-C, long fringe neck two-line sterile line recurrent parent S-D, make respectively hybridization A/B and D/C, must hybridize F
1in generation,, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, F must reestablish diplomatic relations
1, with F
1-ABCDrepresent;
3) plantation F
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1500-1800 strain, carries out respectively drought resisting, cold-resistant, saline alkali tolerance experiments seedling stage, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance, select to have the rice strain listing mark of long fringe neck proterties flowering stage, maturing stage is individual plant sowing respectively, obtains F
3-ABCD;
4) strain plantation F
3-ABCD, recurrent parent S is planted in each strain plantation 40-50 strain simultaneously, selects individual plant and the recurrent parent S hybridization of economical character excellence flowering stage, and recurrent parent S does maternal, must hybridize F
1in generation,, with SF
1-ABCDrepresent;
5) plantation 4) gained SF
1-ABCDseed, selfing obtains SF
2-ABCD, strain plantation SF
2-ABCD, plant recurrent parent S simultaneously, seedling stage is to SF
2-ABCDcarry out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, remove not drought resisting, cold-resistant, the rice shoot of Salt And Alkali Tolerance not, retain the rice shoot of drought resisting, cold-resistant, Salt And Alkali Tolerance;
6) select the SF of economical character excellence flowering stage
2-ABCDindividual plant and recurrent parent S backcross, and recurrent parent S does maternal, obtains 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, plant recurrent parent S simultaneously, seedling stage is to BC
1f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, and the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, selects the individual plant of listing of economical character excellence to continue to backcross with recurrent parent S flowering stage, and recurrent parent S makes female parent, and BC must backcross
2f
1generation;
7) plantation BC
2f
1for seed, selfing obtains BC
2f
2generation, plantation BC
2f
2generation, plant recurrent parent S simultaneously, seedling stage is to BC
2f
2in generation, is carried out respectively drought resisting, cold-resistant, saline alkali tolerance experiments, the rice shoot of listing mark drought resisting, cold-resistant, Salt And Alkali Tolerance, select economical character excellence,, pollen abortion thoroughly individual plant listing mark the most similar to recurrent parent S flowering stage, shift-in temperature is that the climatic cabinate of 23 degree cuts pocket regeneration, observe pollen fertility flowering stage, select the individual plant sowing that pollen fertility is good, ripening rate is high;
8) strain plantation 7) gained individual plant, its economical character, pollen fertility, fertility conversion temperature are investigated to screening, select economical character similar to recurrent parent S, can educate the long fringe neck proterties of phase performance, sterile phase pollen abortion thorough and, single-plant propagation seed that fertility conversion temperature is close with recurrent parent S, be bred as the long fringe neck of high resistance to cold and diseases two-line sterile line.
2. the selection of the long fringe neck of high resistance to cold and diseases according to claim 1 two-line sterile line, is characterized in that: described long fringe neck two-line sterile line recurrent parent S is good fortune eS1 or extensively accounts for 63eS.
3. the selection of the long fringe neck of high resistance to cold and diseases according to claim 1 two-line sterile line, it is characterized in that: described step 5) is to step 8), every step that needs hybridization or sowing, all being arranged in two-line sterile line the flowering stage of its material plantation can educate heading under temperature condition and bloom, the every step that need to identify the pollen fertility of material is all arranged under two-line sterile line fertility conversion lower limit temperature condition ears and blooms, and the procedure that the fertility conversion temperature of material is identified carries out in climatic cabinate.
4. the selection of the long fringe neck of high resistance to cold and diseases according to claim 1 two-line sterile line, is characterized in that: drought-resistant variety is green non-irrigated No. 1, and cold-resistant variety is wild No. of east, and Salt And Alkali Tolerance kind is eastern rice No. four.
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Cited By (10)
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| CN104488699A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of erect panicle, long-stigma and elongated upmost internode two-line male sterile lines |
| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
| CN104642103A (en) * | 2015-03-12 | 2015-05-27 | 福建农林大学 | Breeding method of rice noodles dedicated special rice |
| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
| CN105706913A (en) * | 2016-03-09 | 2016-06-29 | 福建农林大学 | Breeding method of forage-use dry rice dual-purpose genic male sterility line |
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| CN104488702A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of dual-purpose sterile lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice |
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| CN104488702B (en) * | 2015-01-15 | 2016-04-06 | 福建农林大学 | The selection of the glutinous two-line sterile line of purple leaf upright spike length fringe neck |
| CN104488699A (en) * | 2015-01-15 | 2015-04-08 | 福建农林大学 | Breeding method of erect panicle, long-stigma and elongated upmost internode two-line male sterile lines |
| CN104642103A (en) * | 2015-03-12 | 2015-05-27 | 福建农林大学 | Breeding method of rice noodles dedicated special rice |
| CN104642103B (en) * | 2015-03-12 | 2016-07-06 | 福建农林大学 | A kind of selection of rice flour special rice |
| CN104839010A (en) * | 2015-05-21 | 2015-08-19 | 福建金山都发展有限公司 | Breeding method of high-quality disease-resistant high-temperature-resistant rice two-line sterile line |
| CN105706913B (en) * | 2016-03-09 | 2018-01-12 | 福建农林大学 | A kind of selection of feeding upland rice two-line sterile line |
| CN105706913A (en) * | 2016-03-09 | 2016-06-29 | 福建农林大学 | Breeding method of forage-use dry rice dual-purpose genic male sterility line |
| CN106258946A (en) * | 2016-10-28 | 2017-01-04 | 福建农林大学 | A kind of selection of high resistance to cold and diseases glutinous corn variety |
| CN106508664A (en) * | 2016-10-28 | 2017-03-22 | 福建农林大学 | Breeding method of high-stress-resistance sweet corn variety |
| CN106258949A (en) * | 2016-10-28 | 2017-01-04 | 福建农林大学 | A kind of selection of high Resistant glutinous corn variety |
| CN106258949B (en) * | 2016-10-28 | 2018-10-23 | 福建农林大学 | A kind of selection of high Resistant glutinous corn variety |
| CN106258946B (en) * | 2016-10-28 | 2018-10-30 | 巴马万家福瑶药科技发展有限公司 | A kind of selection of high resistance to cold and diseases glutinous corn variety |
| CN110249942A (en) * | 2019-07-29 | 2019-09-20 | 海南广陵高科实业有限公司 | A kind of hybrid rice breeding method |
| CN110249942B (en) * | 2019-07-29 | 2021-07-20 | 海南广陵高科实业有限公司 | Hybrid rice breeding method |
| CN111436365A (en) * | 2020-03-25 | 2020-07-24 | 上海天谷生物科技股份有限公司 | Water-saving drought-resistant rice two-line sterile line cultivation method |
| CN111436365B (en) * | 2020-03-25 | 2023-08-18 | 上海天谷生物科技股份有限公司 | Cultivation method of water-saving drought-resistant rice two-line sterile line |
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