CN104132838B - Gel dyeing and decoloration device - Google Patents
Gel dyeing and decoloration device Download PDFInfo
- Publication number
- CN104132838B CN104132838B CN201410361967.2A CN201410361967A CN104132838B CN 104132838 B CN104132838 B CN 104132838B CN 201410361967 A CN201410361967 A CN 201410361967A CN 104132838 B CN104132838 B CN 104132838B
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- case
- dyeing
- upper strata
- gel
- lower floor
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Abstract
The invention discloses a gel dyeing and decoloration device. The gel dyeing and decoloration device comprises a rectangular box body of which the interior is divided into an upper box, a middle box and a lower box, the upper box and the lower box are symmetrical, each one of the upper box and the lower box is divided into a dyeing chamber, a washing chamber and a decoloration chamber along a length direction, the bottom of each one of the chambers of the upper box is provided with a liquid valve, each one of the chambers at the top of the upper box is provided a sample injection mouth, a side wall of the middle box is provided with an object slide inlet, a drawable drawer-type blocking plate is arranged at the object slide inlet, the drawable drawer-type blocking plate is provided with a sealing ring fitting to the object slide inlet, and the bottom of the middle box corresponding to each one of the chambers of the lower box is provided with a liquid valve. The gel dyeing and decoloration device can reduce contact of an operator with reagents and gel in dyeing and decoloration, simplify the whole dyeing and decoloration processes and realize synchronous dyeing and decoloration of multiple gel object slides. Through inversion of the gel dyeing and decoloration device, novel dyeing and decoloration processes can be carried out.
Description
Technical field
The invention belongs to field of experiment equipment, relate generally to a kind of gel-colored and decoloration device.
Background technology
In the prior art, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) are currently normal
One of detection method, it not only can detect the relative molecular mass of protein, and the separation that can be also used for protein is pure
Change.Conventional coomassie brilliant blue R_250 and G-250 are dyeed, and typically dyeing liquor and gel are placed at the containers such as beaker
In, dyeed in vibration shaking table and decoloured, taken more than 3 hours, dyeing-decolorzing presence is carried out by vibration shaking table time-consuming
Long, inconvenient operation, inefficient, and hold under the arm and take polyacrylamide gel electrophoresis glue easily to produce breakage, lead to experimental result to be subject to
The shortcoming damaged.
Content of the invention
The purpose of the present invention is for deficiency of the prior art, provides a kind of gel-colored and decoloration device, this device
It is effectively reduced the contact with reagent and gel in dyeing with decolorization for the operator, simplify the whole mistake dyeing and decolouring
Journey, and multiple gels load glass plate that can dye simultaneously and decolour；And device is reversed, you can carry out new dyeing and decolorization operations.
The present invention realizes above-mentioned purpose and employed technical scheme comprise that：A kind of gel-colored and decoloration device, long including one
Cube casing, the inside of casing is divided into upper strata case, middle level case and lower floor's case three-decker, and upper strata case and lower floor's case are symmetrically,
Upper strata case and lower floor's case are all partitioned into three parts along its length, are followed successively by dyeing chamber, washing cavities and decolouring chamber；Upper strata case every
Individual cavity bottom is equipped with a Liquid valve, and at the top of upper strata case, the position corresponding to each cavity is respectively equipped with one
Adding mouth with closure, each cavity wall of upper strata case is equipped with sample-adding normal line；One of described middle level case
Load glass plate entrance is offered on the wall of side, carry glass plate entrance be provided with can pull drawer formula baffle plate, drawer formula baffle plate is provided with and carries glass plate
The sealing ring of entrance cooperation, is connected with multiple carriers for placing load glass plate from top to bottom, is carrying inside drawer formula baffle plate
On frame, the both sides away from drawer formula baffle plate one termination are equipped with the catch setting load glass plate for card, are provided with one outside drawer formula baffle plate
Handle；In the bottom of middle level case, corresponding to being respectively equipped with a Liquid valve at each cavity of lower floor's case；Described lower floor case is every
The bottom of individual cavity is respectively equipped with an adding mouth carrying closure.
The top of the bottom of described upper strata case and lower floor's case is respectively horizontal by 3 ° of angles.
Beneficial effects of the present invention
The gel-colored and decoloration device one side that the present invention provides is effectively reduced operator in dyeing and decolorization
In contact with reagent and gel, reduce the harm to operator for the noxious material in operating process, on the other hand this device is effective
Simplify the whole process dyeing and decolouring, and multiple gels load glass plate that can dye simultaneously and decolour, staining efficiency height；With
When, when dyeing liquor and destainer are effective, device is reversed, you can carry out new dyeing and decolorization operations, dyeing liquor and decolouring
Liquid is reusable, save resources, reduces cost.
Brief description
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the front view of middle level of the present invention case internal structure schematic diagram；
Fig. 3 is the top view of middle level of the present invention case internal structure schematic diagram；
Reference：1st, upper strata case, 2, middle level case, 3, lower floor's case, 4, dyeing chamber, 5, washing cavities, 6, decolouring chamber, 7, sample-adding
Mouthful, 8, Liquid valve, 9, sample-adding normal line, 10, drawer formula baffle plate, 11, carry glass plate, 12, carrier, 13, handle, 14, catch,
15th, gel.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described：
A kind of gel-colored and decoloration device, including a rectangular box body, the inside of casing be divided into upper strata case 1, in
Layer case 2 and lower floor's case 3 three-decker, and upper strata case 1 and lower floor's case 3 symmetrical, upper strata case 1 and lower floor's case 3 all along its length between
It is divided into three parts, be followed successively by dyeing chamber 4, washing cavities 5 and decolouring chamber 6；Each cavity bottom of upper strata case 1 is equipped with a liquid
Valve 8, at the top of upper strata case 1, the position corresponding to each cavity is respectively equipped with an adding mouth carrying closure, upper strata
Sample-adding normal line 9 is equipped with each cavity wall of case 1；Load glass plate is offered on one side wall of described middle level case 2 enter
Mouthful, carry glass plate entrance be provided with can pull drawer formula baffle plate 10, drawer formula baffle plate 10 be provided with and carry glass plate entrance cooperation sealing ring,
It is connected with multiple carriers 12 for placing load glass plate 11 from top to bottom inside drawer formula baffle plate 10, on carrier 12, away from
The both sides of drawer formula baffle plate 10 1 termination are equipped with and set, for card, the catch 14 carrying glass plate 11, are provided with a handle outside drawer formula baffle plate 10
Hand 13；In the bottom of middle level case 2, corresponding to being respectively equipped with a Liquid valve at each cavity of lower floor's case 3；Described lower floor case
The bottom of 3 each cavity is respectively equipped with an adding mouth carrying closure.The bottom of described upper strata case 1 and the top of lower floor's case 3
Portion is respectively horizontal by 3 ° of angles.
The using method of the present invention：
1st, prepare before operating
Close the Liquid valve 8 at upper strata case 1 bottom and lower floor's case 3 top respectively, three that turn off lower floor's case 3 bottom add
Sample mouth 7, opens three adding mouths 7 at upper strata case 1 top.
2nd, it is loaded
Add dyeing liquor, destainer and water to corresponding in the dyeing chamber 4 of upper strata case 1, washing cavities 5 and decolouring chamber 6 respectively, all
Add to sample-adding normal line 9.
3rd, place gel
Gel 15 is placed in load glass plate 11, the then drawer formula baffle plate 10 of the load glass plate porch of pull middle level case 2, will
Carry glass plate 11 and be placed on carrier 12, turn off drawer formula baffle plate 10.
4th, dyeing, washing and decolouring
First open the Liquid valve 8 that upper strata case 1 dyes bottom of chamber portion, so that dyeing liquor is entered middle level case and cover gel 15, so
After place the device in and start on shaking table to dye, after dyeing terminates, slow down shaking table and shake speed, close upper strata case 1 and dye bottom of chamber portion
Liquid valve 8, opens the Liquid valve 8 at dyeing chamber 4 top of lower floor's case 3, dyeing liquor is flowed in the dyeing chamber 4 of lower floor's case 3,
Close the Liquid valve 8 having already turned on, that is, complete dyeing course.Open the Liquid valve 8 of the washing cavities 5 of upper strata case 1, so that water is entered
Enter middle level case and cover gel 15, after washing, close the Liquid valve of upper strata case 1 washing cavities, open lower floor's case 3 washing top of chamber
Liquid valve 8, move the water to flow in the washing cavities 5 of lower floor's case 3, close the Liquid valve 8 that has already turned on, complete washing process.
Open the Liquid valve 8 in the decolouring chamber 6 of upper strata case 1, so that destainer is entered middle level case and cover gel 15, close after decolouring and shake
Bed, closes the Liquid valve in upper strata case 1 decolouring chamber, opens the Liquid valve 8 of lower floor's case 3 decolouring top of chamber, so that destainer is flowed into
In the decolouring chamber 5 of lower floor's case 3, close the Liquid valve 8 having already turned on, complete decolorization.Take out and carry glass plate 11, that is, complete to contaminate
Color and decolorization operations.
When next time uses, this device is reversed, repeats.When dyeing liquor and destainer lost efficacy, change.
The gel-colored and decoloration device one side that the present invention provides is effectively reduced operator in dyeing and decolorization
In contact with reagent and gel, reduce the harm to operator for the noxious material in operating process, on the other hand this device is effective
Simplify the whole process dyeing and decolouring, and multiple gels load glass plate that can dye on the same day and decolour；Meanwhile, in dyeing liquor and
When destainer is effective, device is reversed, you can carry out new dyeing and decolorization operations, dyeing liquor and destainer are reusable,
Save resources, reduces cost.
Claims (2)
1. a kind of gel-colored and decoloration device it is characterised in that：Including a rectangular box body, the inside of casing is divided into
Layer case（1）, middle level case（2）With lower floor's case（3）Three-decker, and upper strata case（1）With lower floor's case（3）Symmetrically, upper strata case（1）With under
Layer case（3）All it is partitioned into three parts along its length, be followed successively by dyeing chamber（4）, washing cavities（5）With decolouring chamber（6）；Upper strata case
（1）Each cavity bottom be equipped with a Liquid valve（8）, in upper strata case（1）Top, corresponding to the position of each cavity
It is respectively equipped with an adding mouth carrying closure, upper strata case（1）Each cavity wall on be equipped with sample-adding normal line（9）；
Described middle level case（2）A side wall on offer load glass plate entrance, carry glass plate entrance be provided with can pull drawer formula baffle plate
（10）, drawer formula baffle plate（10）It is provided with and carries the sealing ring of glass plate entrance cooperation, drawer formula baffle plate（10）Inner side connects from top to bottom
Have multiple for place carry glass plate（11）Carrier（12）, in carrier（12）On, away from drawer formula baffle plate（10）One termination
Both sides are equipped with and set load glass plate for card（11）Catch（14）, drawer formula baffle plate（10）Outside is provided with a handle（13）；In
Layer case（2）Bottom, corresponding to lower floor's case（3）Each cavity at be respectively equipped with a Liquid valve；Described lower floor case（3）Often
The bottom of individual cavity is respectively equipped with an adding mouth carrying closure, and lower floor's case（3）On the position of adding mouth and upper strata
Case（1）On the position of adding mouth be located at the relative both sides of casing respectively.
2. one kind as claimed in claim 1 is gel-colored and decoloration device it is characterised in that：Described upper strata case（1）Bottom
With lower floor's case（3）Top respectively horizontal by 3 ° of angles.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361967.2A CN104132838B (en) | 2014-07-28 | 2014-07-28 | Gel dyeing and decoloration device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410361967.2A CN104132838B (en) | 2014-07-28 | 2014-07-28 | Gel dyeing and decoloration device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104132838A CN104132838A (en) | 2014-11-05 |
| CN104132838B true CN104132838B (en) | 2017-02-15 |
Family
ID=51805604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410361967.2A Expired - Fee Related CN104132838B (en) | 2014-07-28 | 2014-07-28 | Gel dyeing and decoloration device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104132838B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568558A (en) * | 2015-01-09 | 2015-04-29 | 贵州大学 | Combining device for dyeing polyacrylamide gel |
| CN106053188B (en) * | 2016-06-24 | 2019-01-29 | 广东医科大学 | A kind of gel dye decoloration integration apparatus and its control method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317710A (en) * | 1979-10-18 | 1982-03-02 | Olympus Optical Co., Ltd. | Coloring-decoloring-drying apparatus for electrophoresis |
| WO2010048631A2 (en) * | 2008-10-24 | 2010-04-29 | Biomicro, Inc. | Modular system for performing laboratory protocols and associated methods |
| CN202533302U (en) * | 2012-04-10 | 2012-11-14 | 中国农业科学院兰州畜牧与兽药研究所 | Combined high-flux gel dyeing cabinet |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN204142547U (en) * | 2014-07-28 | 2015-02-04 | 河南科技大学 | A kind of gel-colored and decoloration device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005098767A (en) * | 2003-09-24 | 2005-04-14 | Jokoh Co Ltd | Apparatus for staining small amount of protein by argentation |
| JP2008082854A (en) * | 2006-09-27 | 2008-04-10 | Hitachi High-Tech Science Systems Corp | Method and device for dyeing and decoloring gel |
-
2014
- 2014-07-28 CN CN201410361967.2A patent/CN104132838B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4317710A (en) * | 1979-10-18 | 1982-03-02 | Olympus Optical Co., Ltd. | Coloring-decoloring-drying apparatus for electrophoresis |
| WO2010048631A2 (en) * | 2008-10-24 | 2010-04-29 | Biomicro, Inc. | Modular system for performing laboratory protocols and associated methods |
| CN202533302U (en) * | 2012-04-10 | 2012-11-14 | 中国农业科学院兰州畜牧与兽药研究所 | Combined high-flux gel dyeing cabinet |
| CN103575584A (en) * | 2013-08-12 | 2014-02-12 | 北京林业大学 | Gel electrophoresis dyeing device |
| CN204142547U (en) * | 2014-07-28 | 2015-02-04 | 河南科技大学 | A kind of gel-colored and decoloration device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104132838A (en) | 2014-11-05 |
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