CN104130078A - Bio-organic fertilizer for continuously cropping watermelons - Google Patents
Bio-organic fertilizer for continuously cropping watermelons Download PDFInfo
- Publication number
- CN104130078A CN104130078A CN201410424096.4A CN201410424096A CN104130078A CN 104130078 A CN104130078 A CN 104130078A CN 201410424096 A CN201410424096 A CN 201410424096A CN 104130078 A CN104130078 A CN 104130078A
- Authority
- CN
- China
- Prior art keywords
- bio
- organic fertilizer
- continuous cropping
- plantation
- fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a bio-organic fertilizer for continuously cropping watermelons. Bacillus subtilis and earthworm cast are used as raw materials, the bacillus subtilis is prepared by using a solid fermentation process and is inoculated on the earthworm cast according to the quantity of 50kg/ton, and the bacillus subtilis and the earthworm cast are sufficiently stirred and uniformly mixed to obtain the bio-organic fertilizer for continuously cropping watermelons; the bio-organic fertilizer contains 0.95-2.5% of total nitrogen, 1.1-2.9% of total phosphorus, 0.96-2.2% of total potassium, 25-38% of organic matters, 21-40% of humics and 0.5-5 hundred millions of effective microbial communities and also contains abundant mineral microelements and amino acid. The bio-organic fertilizer can be applied as a base fertilizer and an additional fertilizer, and the dosage of the bio-organic fertilizer is 1/8-1/10 of the conventional fertilizing amount.
Description
Technical field
The invention belongs to microbial fertilizer field, specifically a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping.
Background technology
Watermelon can be rated as " king in melon ", clearly quenches one's thirst, and taste sugariness succulence, is good fruit in midsummer, is loved by the people.Watermelon is avoided continuous cropping, from toxic action, is the important factor that watermelon produces continuous cropping obstacle, and in existing known watermelon is phenylformic acid and styracin from poisonous substance matter.Current, the common method that overcomes continuous cropping obstacle of watermelon has: chemical prevention, physical control and integrated pest control in agriculture.Physical control mainly utilizes the sun to close disinfection soil, but effect is subject to such environmental effects larger, poor stability; Integrated pest control in agriculture mainly utilizes crop rotation, and the cycle is long, cannot meet the need of market; Chemical prevention (waiting bitterly for chemical agent fumigating soil with monobromethane, chlorination) mainly be take as main in most melons ground, chemical prevention preventive effect is good, some plot preventive effect can reach 100%, but monobromethane is serious to depletion of the ozone layer, trichloronitromethane is a kind of highly toxic fumigant, the mankind's health and environment are caused to serious harm, and easily develop immunity to drugs.
Through the literature search of prior art is found, the existing method that overcomes continuous cropping obstacle of watermelon, as Chinese patent CN200910233576, patent name is: continuous cropping cucumber, the biocontrol strains of watermelon blight and microorganism organic fertilizer thereof, this invention is take a kind of organic fertilizer of trichoderma harziarum as raw material manufacture, continuous cropping watermelon blight is being prevented to have positive effect, but soil-borne disease is a reason of continuous cropping obstacle of watermelon, the fertilizer of this invention does not successfully manage measure for watermelon from toxic action and soil secondary salinization, the effect of alleviating continuous cropping obstacle of watermelon is undesirable.Separately there is Chinese invention patent CN201410022691 to disclose a kind of organic watermelons continuous cropping high yield compound bacterial fertilizer, its raw material comprises 60% choline chloride 60, and watermelon is extremely responsive to chlorion, and this invention adds choline chloride 60, not only continuous cropping obstacle of watermelon can not be alleviated, also quality of watermelon production declining can be caused.
Summary of the invention
The object of the invention is to solve the problem that prior art exists, a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping is provided, raw material is subtilis and wormcast, by reducing soil salt, accumulates, and improves organic content, improving soil microorganisms forms, increase actinomycetes content in soil, buffering soil acidity or alkalinity, suppresses phenolic acids amicine, reach alleviation continuous cropping obstacle of watermelon, improve the object of quality of watermelon and output.
For achieving the above object, technical scheme of the present invention is as follows:
A. the solid-state fermentation process of subtilis is: (1) substratum: wheat bran 19.5g, rice hulls 0.5g, dregs of beans 1.0g, water 22mL; (2) culture condition: initial pH is 7.0, inoculation time 20h, inoculum size is 0.9mL; According to this technique fermentation, the viable count of subtilis is up to 43,500,000,000/g.
B. liquid seeds is cultivated: every 250mL triangular flask loading amount 30mL liquid seed culture medium, sterilizing 20min under 121 ℃ of conditions.After cooling, from subtilis inclined-plane, connect a ring bacterial classification to liquid seed culture medium, put 37 ℃ of temperature control shaking tables, under 220r/min, cultivate 24h.
C. triangular flask solid state fermentation: take solid fermentation culture medium raw material according to above-mentioned formula, add tap water to stir, amount of water is 20～22mL, with " hold webs and have free-water; but can not drip " be standard, solid medium after stirring evenly is transferred in 250mL triangular flask, substratum is through 121 ℃ of sterilizing 30min, after cooling, inoculate into 3% (V/m, V-liquid spawn volume number, the quality of m-solid fermentation substratum) subtilis liquid seeds, then put static cultivation in 37 ℃ of incubators, between time pat, it is evenly grown, cultivate 48h.Then in 60 ℃ of oven dry, pulverizing.
D. the subtilis of above-mentioned solid state fermentation is seeded on wormcast by the amount of 50kg/ ton, fully stirring and evenly mixing is the bio-organic fertilizer for the plantation of watermelon continuous cropping.In this bio-organic fertilizer, total nitrogen content 0.95～2.5%, content of tatal phosphorus 1.1～2.9%, full potassium content 0.96～2.2%, organic 25～38%, humus 21～40%, profitable strain number reaches 5,000 ten thousand～500,000,000/g, contains abundant mineral trace element and amino acid.
Described bio-organic fertilizer is exclusively used in the plantation of watermelon continuous cropping.Usage and consumption are as follows:
Usage: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its usage, with conventional organic fertilizer, can be used as base fertilizer and topdresses and use.
Consumption: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its consumption is advisable with 1/8～1/10 of conventional fertilizer application amount.
The present invention has following beneficial effect:
(1) a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, for main three factors that cause continuous cropping obstacle: soil-borne disease, from toxic action and soil secondary salinization, prevent and treat with a definite target in view, prevention effect is remarkable, and greatly degree is alleviated continuous cropping obstacle of watermelon;
(2) this fertilizer is bio-organic fertilizer, contain and abundant take subtilis as main profitable strain (5,000 ten thousand～500,000,000/g of content), pathogenic germ Fusarium oxysporum for soil-borne disease especially watermelon blight has obvious restraining effect, can effectively prevent continuous cropping watermelon blight;
(3) this fertilizer is bio-organic fertilizer, contain abundant organic matter (content 25～38%), can reduce soil salt accumulation, being converted into soluble salt is absorbed, Simultaneous Stabilization soil physico-chemical property, buffering acid basicity, alleviate watermelon allelochemical from toxic action, alleviate rehabilitating soil secondary salinization;
(4) owing to being bio-organic fertilizer, not a series of safety and the pollution problem because using chemical fertilizer and agricultural chemicals to bring completely, thereby be conducive to the nuisanceless production of watermelon, this bio-organic fertilizer also has high fertilizer efficiency simultaneously, amount of application is only 1/8～1/10 of conventional fertilizer application amount, save production cost, also improve the yield and quality, can increase production extra earning for melon grower.
Embodiment
Embodiment 1: strain culturing
A. the solid-state fermentation process of subtilis is:
(1) substratum: wheat bran 19.5g, rice hulls 0.5g, dregs of beans 1.0g, water 22mL;
(2) (2) culture condition: initial pH is 7.0, inoculation time 20h, inoculum size is 0.9mL.
B. liquid seeds is cultivated: every 250mL triangular flask loading amount 30mL liquid seed culture medium, sterilizing 20min under 121 ℃ of conditions.After cooling, from subtilis inclined-plane, connect a ring bacterial classification to liquid seed culture medium, put 37 ℃ of temperature control shaking tables, under 220r/min, cultivate 24h.
C. triangular flask solid state fermentation: take solid fermentation culture medium raw material according to above-mentioned formula, add tap water to stir, amount of water is 20～22mL, with " hold webs and have free-water; but can not drip " be standard, solid medium after stirring evenly is transferred in 250mL triangular flask, substratum is through 121 ℃ of sterilizing 30min, after cooling, inoculate into 3% (V/m, V-liquid spawn volume number, the quality of m-solid fermentation substratum) subtilis liquid seeds, then put static cultivation in 37 ℃ of incubators, between time pat, it is evenly grown, cultivate 48h.Then in 60 ℃ of oven dry, pulverizing.
Embodiment 2: microbial inoculum is made
The subtilis of above-mentioned solid state fermentation is seeded on wormcast by the amount of 50kg/ ton, and fully stirring and evenly mixing is the bio-organic fertilizer for the plantation of watermelon continuous cropping.In this bio-organic fertilizer, total nitrogen content 0.95～2.5%, content of tatal phosphorus 1.1～2.9%, full potassium content 0.96～2.2%, organic 25～38%, humus 21～40%, profitable strain number reaches 5,000 ten thousand～500,000,000/g, contains abundant mineral trace element and amino acid.
Embodiment 3: usage and consumption
(1) usage: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its usage, with conventional organic fertilizer, can be used as base fertilizer and topdresses and use.
(2) consumption: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its consumption is advisable with 1/8～1/10 of conventional fertilizer application amount.
Embodiment 4: greenhouse pot culture test
Test soil is watermelon continuous cropping soil, and continuous cropping obstacle is serious, and especially blight morbidity is serious, shows as plant wither.Process as follows:
CK: watermelon continuous cropping soil is used common organic fertilizer; T: watermelon continuous cropping soil is used bio-organic fertilizer of the present invention.
Each is processed 10 times and repeats, and repeats 1000g continuous cropping soil/basin at every turn, and CK executes the common organic fertilizer of 80g, T executes 10g bio-organic fertilizer of the present invention, after evenly mixing, transplant watermelon seedling, within the 28th day, recording average attack rate CK is that 97.2%, T is 2.3% (in Table 1).
Two, table 1 is processed watermelon seedling sickness rate, improving activity of root system and plant dry weight
Test-results shows: use bio-organic fertilizer of the present invention, can effectively alleviate continuous cropping obstacle of watermelon, suppressing watermelon blight occurs, activating soil, improves improving activity of root system, reduces soil salt content, improve utilization rate of fertilizer, accelerate dry-matter accumulation, improve the yield of watermelon and quality, for the plantation of watermelon continuous cropping, there is remarkable income.
Claims (5)
1. for a bio-organic fertilizer for watermelon continuous cropping plantation, it is characterized in that, bio-organic fertilizer of the present invention is to obtain after the abundant stirring and evenly mixing of subtilis and wormcast.
2. a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping as claimed in claim 1, is characterized in that, described bio-organic fertilizer is inoculated on wormcast by the amount of 50kg/ ton by subtilis.
3. a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping as claimed in claim 1, is characterized in that, described wormcast purity is 100%.
4. a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping as claimed in claim 1, is characterized in that, described subtilis is obtained by solid fermentation, and its solid fermentation process is as follows:
Substratum: wheat bran 19.5g, rice hulls 0.5g, dregs of beans 1.0g, water 22mL;
Culture condition: initial pH is 7.0, inoculation time 20h, inoculum size is 0.9mL;
Liquid seeds is cultivated: every 250mL triangular flask loading amount 30mL liquid seed culture medium, and sterilizing 20min under 121 ℃ of conditions, connects a ring bacterial classification to liquid seed culture medium from subtilis inclined-plane after cooling, put 37 ℃ of temperature control shaking tables, under 220r/min, cultivates 24h;
Triangular flask solid state fermentation: take solid fermentation culture medium raw material according to above-mentioned formula, add tap water to stir, amount of water is 20～22mL, with " hold webs and have free-water; but can not drip " be standard, solid medium after stirring evenly is transferred in 250mL triangular flask, substratum is through 121 ℃ of sterilizing 30min, after cooling, inoculate into 3% (V/m, V-liquid spawn volume number, the quality of m-solid fermentation substratum) subtilis liquid seeds, then put static cultivation in 37 ℃ of incubators, between time pat, it is evenly grown, cultivate 48h.Then in 60 ℃ of oven dry, pulverizing.
5. a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping as claimed in claim 1, is characterized in that, usage and the consumption of described bio-organic fertilizer are as follows:
Usage: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its usage, with conventional organic fertilizer, can be used as base fertilizer and topdresses and use.
Consumption: a kind of bio-organic fertilizer for the plantation of watermelon continuous cropping of the present invention, its consumption is advisable with 1/8～1/10 of conventional fertilizer application amount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410424096.4A CN104130078B (en) | 2014-08-26 | 2014-08-26 | A kind of bio-organic fertilizer for the plantation of Citrullus vulgaris continuous cropping |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410424096.4A CN104130078B (en) | 2014-08-26 | 2014-08-26 | A kind of bio-organic fertilizer for the plantation of Citrullus vulgaris continuous cropping |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104130078A true CN104130078A (en) | 2014-11-05 |
| CN104130078B CN104130078B (en) | 2016-08-24 |
Family
ID=51802987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410424096.4A Expired - Fee Related CN104130078B (en) | 2014-08-26 | 2014-08-26 | A kind of bio-organic fertilizer for the plantation of Citrullus vulgaris continuous cropping |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104130078B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009861A (en) * | 2015-06-25 | 2015-11-04 | 李春燕 | Successive-cropping planting method of watermelons |
| CN107094571A (en) * | 2017-05-09 | 2017-08-29 | 广东药科大学 | The implantation methods of Pogostemon cablin seedling under a kind of Continuous Mono-cropping |
| CN107950316A (en) * | 2017-12-05 | 2018-04-24 | 登封市绿奥农林科技有限公司 | A kind of method for overcoming open country continuous cropping obstacle of watermelon |
| CN109836190A (en) * | 2019-02-18 | 2019-06-04 | 曹翠玲 | A method of biocontrol bacterial fertilizer is prepared using biocontrol bacteria and eliminates muskmelon continuous cropping obstacle |
| CN110468056A (en) * | 2019-08-14 | 2019-11-19 | 安徽旭辰生物科技有限公司 | It is a kind of prevent and treat watermelon blight Trichoderma and its application |
| CN111758743A (en) * | 2020-07-10 | 2020-10-13 | 山东农业大学 | Soil bactericide for preventing and controlling apple or pear continuous cropping obstacle and application thereof |
-
2014
- 2014-08-26 CN CN201410424096.4A patent/CN104130078B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009861A (en) * | 2015-06-25 | 2015-11-04 | 李春燕 | Successive-cropping planting method of watermelons |
| CN107094571A (en) * | 2017-05-09 | 2017-08-29 | 广东药科大学 | The implantation methods of Pogostemon cablin seedling under a kind of Continuous Mono-cropping |
| CN107950316A (en) * | 2017-12-05 | 2018-04-24 | 登封市绿奥农林科技有限公司 | A kind of method for overcoming open country continuous cropping obstacle of watermelon |
| CN109836190A (en) * | 2019-02-18 | 2019-06-04 | 曹翠玲 | A method of biocontrol bacterial fertilizer is prepared using biocontrol bacteria and eliminates muskmelon continuous cropping obstacle |
| CN110468056A (en) * | 2019-08-14 | 2019-11-19 | 安徽旭辰生物科技有限公司 | It is a kind of prevent and treat watermelon blight Trichoderma and its application |
| CN111758743A (en) * | 2020-07-10 | 2020-10-13 | 山东农业大学 | Soil bactericide for preventing and controlling apple or pear continuous cropping obstacle and application thereof |
| CN113331213A (en) * | 2020-07-10 | 2021-09-03 | 山东农业大学 | Soil bactericide for preventing and controlling apple or pear continuous cropping obstacle and application thereof |
| CN113331213B (en) * | 2020-07-10 | 2023-03-28 | 山东农业大学 | Soil bactericide for preventing and controlling apple or pear continuous cropping obstacle and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104130078B (en) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106495879B (en) | A kind of preparation method of green peel of walnut residue organic fertilizer | |
| CN102976801B (en) | Method for producing functional microorganism organic fertilizer by using food residue | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN101874445B (en) | Method for culturing organic Chinese chives | |
| CN104130078A (en) | Bio-organic fertilizer for continuously cropping watermelons | |
| CN104447001A (en) | Special compound microbial fertilizer for rice | |
| CN104086242A (en) | Biological organic fertilizer and preparation method thereof | |
| CN106518400A (en) | Method for preparing flower substrate from agricultural wastes | |
| CN106497836A (en) | A kind of complex micro organism fungicide of fermented stalk and its preparation method and application | |
| CN103467185A (en) | Biological compound organic bacterial fertilizer | |
| CN105367217A (en) | Compound microorganism humic acid fertilizer special for organic cultivation and preparation method thereof | |
| CN107711891B (en) | A method of prevention matrimony vine root rot is extremely set | |
| CN106747804A (en) | A kind of Methylotrophic gemma bacillus agent and preparation method thereof | |
| CN105693395A (en) | Organic fertilizer for improving saline soil and preparation method thereof | |
| CN102674986A (en) | Microbial organic fertilizer with moisture and fertility retention functions and preparation method of microbial organic fertilizer | |
| CN102276351B (en) | Multifunctional total-nutrient fertilizer and its production method | |
| CN105964680A (en) | Coastal saline continuous cropping cotton soil ecological restoration agent and preparing method and application thereof | |
| CN106977324A (en) | A kind of preparation method of cow dung organic fertilizer | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN103724058B (en) | Microbial fermentation functional organic compound fertilizer and production method thereof | |
| CN103525741A (en) | Complex microbial inoculant for greenhouse vegetable production and preparation and application thereof | |
| CN110521735A (en) | Dedicated root rot prevention and treatment microbial inoculum of a kind of highland barley and preparation method thereof | |
| CN102515975B (en) | Tobacco microorganism organic fertilizer | |
| CN106518527A (en) | Biological fertilizer prepared from tea leaves and preparation method of biological fertilizer | |
| CN103708960B (en) | A kind of liquid composite microbic bacterial fertilizer and production method and purposes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: He Fengbao Inventor before: Jiang Hanjun |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160706 Address after: Huachi County of Gansu province Qingyang city 745600 Town Street No. 6 soft far Applicant after: He Fengbao Address before: Chengyi Zhen Hong Bao Zi Cun 730726 Gansu province Baiyin Huining County Guo Club No. 119 room 2 Applicant before: Jiang Hanjun |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160824 Termination date: 20210826 |