CN104126868A - Preparation method of natural perfume deriving from porcini and tobacco aroma enhancement application thereof - Google Patents
Preparation method of natural perfume deriving from porcini and tobacco aroma enhancement application thereof Download PDFInfo
- Publication number
- CN104126868A CN104126868A CN201410344593.3A CN201410344593A CN104126868A CN 104126868 A CN104126868 A CN 104126868A CN 201410344593 A CN201410344593 A CN 201410344593A CN 104126868 A CN104126868 A CN 104126868A
- Authority
- CN
- China
- Prior art keywords
- natural perfume
- cigarette
- preparation
- bolete
- perfume material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fats And Perfumes (AREA)
Abstract
The invention discloses a preparation method of natural perfume deriving from porcini. The method includes the following steps that sterilization treatment is conducted on porcini fruiting bodies, then Galactomyces candidum is added for fermenting, and accordingly the natural perfume is obtained; optionally, solvent is added to extract the natural perfume, the extractive is concentrated, and thus natural perfume concentrate is obtained. The natural perfume or the natural perfume concentrate is added into a tobacco product, so that a special aroma is given to the tobacco product, the cigarette flavor is increased obviously, the sweet feeling and delicacy are increased, and the organoleptic quality is improved obviously. In addition, the release amount of tobacco specific N-nitrosamine in smoke of a traditional cigarette and a novel cigarette (heating and non-combustion type cigarette) can be lowered remarkably in the natural perfume.
Description
Technical field
The invention belongs to tobacco spice field.
Background technology
Along with the growing interest to cigarette consumption person health, and Tobacco Control tide is in global rise, and reducing cigarette tar and reducing becomes the common recognition of whole world tobacco business; Meanwhile, along with cigarette industry technology is constantly brought forth new ideas, on world market, the tar content of cigarette product progressively reduced since 60 years, and in China, the research and development of Chinese style low-coke tar cigarette product and production and sales have become the inexorable trend of China's tobacco business development.But, when tar content in cigarette reduces, perfume quantity, the concentration of cigarette reduce greatly, and what directly affect cigarette sucks comfort level and physiology satisfaction.Especially it should be noted that the production and consumption of China's cigarette is taking flue-cured tobacco as main, tobacco planting area and total output all position occupy first place in the world; And Virginian-type cigarette sugar content is high, there is very high sugared nitrogen ratio, free nicotine ratio is on the low side, flue gas is soft, therefore, Chinese style low-coke tar cigarette to fall burnt difficulty larger, the insufficient fragrance problem of flue-cured tobacco is especially outstanding.In a way, compensating aroma has become a technical bottleneck of Chinese style low-coke tar cigarette goods development.
Meanwhile, traditional cigarette (or say tradition and light type cigarette), as the main product form of tobacco consumption, has the history of more than 100 year.Along with transformation, the quick progress of science and technology and the continuous quickening of commodity upgrading speed of people life style and consumption idea, tobacco consumption person's demand and focus constantly change, be subject to the impact of the factors such as tobacco control, public opinion simultaneously, the development of traditional cigarette is subject to increasing restriction, and the appearance of new cigarette has become inevitable.Since the eighties in last century, international tobacco company has just started the research and development of new cigarette, and research and development of products dynamics in recent years continues to increase, and described new cigarette goods comprise electric heating type cigarette, electronic cigarette, smokeless tobacco articles for example chewing tobacco, snuff etc.But compared with traditional cigarette, still there is larger gap in the new cigarette goods that appeared on market at present both at home and abroad.Wherein, the most significant deficiency is the jealous worse of new cigarette goods, more allows people unacceptable than traditional ultra-low tar cigarette.Therefore, improve new cigarette goods jealous be one of the main direction of the scientific and technical innovation of new cigarette goods and research.
Contained nitrosamines material in the flue gas that the distinctive N-nitrosamine of tobacco produces while being a kind of heating or burning tobacco product, it mainly contains four kinds of forms: N-nitrosonornicotine (NNN), N-nitroso anabasine (NAT), N-nitroso anabasine (NAB), 4-(methyl nitrosamino-)-1-(3-pyridine radicals)-1 butanone (NNK).All have toxicity and carcinogenicity, therefore, cigarette enterprise all will reduce the content of the distinctive nitrosamines material of this tobacco in tobacco product by every means both at home and abroad.
Object of the present invention is exactly to have studied a kind of natural perfume material based on bolete tunning of uniqueness in order to improve the jealous of tobacco product, and it can obviously improve the aromatic property of tobacco product.In addition, inventor is also surprised to find that, this natural perfume material can also reduce the content of the distinctive N-nitrosamines of tobacco material effectively.
Summary of the invention
The invention provides a kind of preparation method of the natural perfume material that comes from bolete, comprise the following steps: bolete fructification is carried out to sterilization treatment, after adding again Geotrichum (Galactomyces candidum) to ferment, obtain described natural perfume material.
Optionally, above-mentioned natural perfume material can also, further through solvent extraction and concentrated, obtain natural perfume material concentrate.
Wherein said bolete is selected from the edible bolete in Boletaceae and pinecone Boletaceae.Bolete is the general designation of the fungi such as Boletaceae (Boletaceae) and pinecone Boletaceae (Strobilomycetaceae), wherein except minority kind is poisonous or bitter can not eat, and most of all edibles.Bolete is because of meat plumpness, the utmost point is gained the name like beef liver, it is famous and precious rare wild edible fungus, for one of " four large bacterium kings ", mainly contain Suillus albidipes (Peck) Sing (Boletus edulis), Boletus luridus (Boletus Luridus), (Boletus aereus) is several for Boletus aereus.Representational Suillus albidipes (Peck) Sing is subordinate to Boletaceae, have another name called cepe, it is good wild edible fungus, it is one of most important economic edible mushroom in Yunnan Province, research shows, 8 seed amino acids that Suillus albidipes (Peck) Sing contains needed by human, in every 100g dry product protein content up to 27.8g, carbohydrate 53.3g.Boletus luridus and Boletus aereus also have same rich in protein content.
The described Geotrichum (Galactomyces candidum) using in the present invention, separates from Baoshan, Yunnan and produces Turkish tobaccos tobacco leaf, is provided by School of Life Sciences, Yunnan University.
The process conditions of wherein said fermentation are as follows: 20 DEG C～35 DEG C of fermentation temperatures, fermentation time 6～12h.
More particularly, the preparation method of the natural perfume material that comes from bolete of the present invention is as follows:
1. bolete biofermentation pre-treatment: bolete fructification is pulverized, is dried, and gained bolete powder is through wet heating sterilizing, and sterilising conditions is: process 20min～60min for 80 DEG C～120 DEG C;
2. fermentation strain is cultivated: select Geotrichum (Galactomyces candidum), be inoculated in culture medium, constant temperature culture 12h～36h at 25 DEG C～35 DEG C, on strain cultures after activation, add aseptic culture fluid, lawn is washed down with transfer needle, then bacterial strain is accessed in the seed nutrient solution of sterilizing, shaking table is cultivated 24h～48h;
3. fermentation: seed nutrient solution is inoculated in aseptic bolete powder to constant temperature culture 6h～12h at 25 DEG C～35 DEG C in the ratio of 1:0.5～2; Described in obtaining after fermentation, come from the natural perfume material of bolete.This natural perfume material can directly spray in tobacco product and use, also can be further after extraction and concentration, then spray in tobacco product and use.
4. optional extraction-concentration step: add ethanol water (70%～85% in tunning, wt%) refluxing extraction three times, each 1～3h that extracts, extract merges reduced pressure concentration, obtains crude extract, and crude extract adds high dense ethanol water (90%～100%, wt%), remove insoluble matter, and concentrated, obtain described natural perfume material concentrate.
The invention still further relates to described natural perfume material for the purposes to tobacco product flavouring.This natural perfume material that adds proper proportion in tobacco product (lighting type cigarette, electrical heating cigarette, electronic cigarette, smokeless tobacco), can significantly improve its organoleptic quality.Adding proportion can need to be selected according to the local flavor of cigarette, is generally 0.01～0.1% of tobacco product weight
Unexpectedly, in experiment, also find, add natural perfume material of the present invention in tobacco product after, can also reduce the distinctive content of nitrosamines of tobacco.Therefore, the invention still further relates to this natural perfume material and light for reducing tradition the purposes of the burst size of the peculiar N-nitrosamine of tobacco in the flue gas of type cigarette and new cigarette (or say heating but not burning type cigarette comprises electronic cigarette and electric heating type cigarette).
The present invention mainly contains following advantage: this is derived from the natural perfume material of bolete (1), not only can give tobacco product flavouring, can also improve the organoleptic quality of the many aspects of tobacco product; (2) adopt biological fermentation process to prepare this natural natural perfume material, can transform efficiently the abundant beneficiating ingredient of bolete, reaction condition gentleness, and production process do not adopt organic catalyst and toxic organic solvent, avoided the pollution of metal ion and toxic organic solvent; (3) described natural perfume material can reduce tobacco-specific nitrosamine (TSNA) burst size in traditional cigarette, electronic cigarette and electric heating type cigarette smoke, the harm reduction effect acquiring a certain degree to a certain extent; (4) this method does not change the original performance of cigarette, does not change the original processing technology of cigarette, has more actual using value and meaning.
Brief description of the drawings
Nothing.
Detailed description of the invention
Below in conjunction with specific embodiment, be described in further detail the present invention.But they are not limitation of the invention:
In an embodiment, use different process conditions and raw material, prepared natural perfume material of the present invention.And select Yunnan brand cigarette as blank cigarette, press GB/T5606.1 and extract laboratory sample, prepare respectively blank cigarette sample 1# and 2#, then add respectively the natural perfume material feed liquid of different proportion, this feed liquid is made up of the natural perfume material of following embodiment gained of the present invention, before smokeing panel test, regulates sample moisture according to GB/T16447.Add standard and the method for the technique effect evaluation of natural perfume material of the present invention front and back: evaluate aesthetic quality's improvement according to " cigarette " GB5606.4-2005 Sensory Quality of Cigarette technical requirement (table 1), under this requirement, the aesthetic quality of cigarette product generally includes: gloss, fragrance, harmony, assorted gas, excitant and six technical requirements of pleasant impression; , in each index, be wherein all that more high organoleptic's quality is better for score value.
Table 1 " cigarette " GB5606.4-2005 Sensory Quality of Cigarette technical requirement
In addition, analyzing four kinds of peculiar N-nitrosamine in combined instrument (GC-TEA) quantitative analysis detection cigarette smoke according to the gas-chromatography in the mensuration of tobacco business standard YC/T184-2004 tobacco and the peculiar N-nitrosamine of tobacco product tobacco-heat energy is N-nitrosonornicotine (NNN), N-nitroso anabasine (NAT), N-nitroso anabasine (NAB), the burst size of 4-(methyl nitrosamino-)-1-(3-pyridine radicals)-1 butanone (NNK).
Embodiment 1:
Suillus albidipes (Peck) Sing (Boletus edulis) fructification low temperature drying, pulverizes and uses 100 DEG C of wet heating to process 30min; In Geotrichum (Galactomyces candidum) after activation, add without bacterial nutrient solution, lawn is washed down with transfer needle, then this bacterium is accessed simultaneously in the seed nutrient solution of sterilization treatment, shaking table is cultivated 24h (160r/min, 28 DEG C); Then, seed culture fluid is inoculated in the Suillus albidipes (Peck) Sing powder after sterilizing and is fermented in the ratio of 1:1,30 DEG C of fermentation temperatures, fermentation time is 12h.Tunning is the natural perfume material that is derived from bolete of the present invention.To the ethanol water refluxing extraction three times that adds 80wt% in tunning, each 1.5h, merge extract concentrated, refining through straight alcohol back dissolving again, remove insoluble matter, obtain through refining natural perfume material, this natural perfume material is added in sample cigarette according to different proportion, and the effect in cigarette is evaluated to it, evaluation result is in table 2.
The effect of table 2 natural perfume material to Sensory Quality of Cigarette
Result shows, in cigarette sample, add appropriate ratio this natural perfume material can by give its alcohol and the fragrant note of wine, increase flue gas concentration, promoting or transferring its sweet sense and exquisiteness, thereby obviously improve its suction quality.In addition, surprisingly, add after this natural perfume material, the burst size of four kinds of peculiar N-nitrosamine in flue gas has had obvious reduction, sees following table 3.
The effect (ng/cig) that table 3 natural perfume material discharges the peculiar N-nitrosamine of tobacco in cigarette smoke
Embodiment 2:
Boletus luridus (Boletus Luridus) fructification low temperature drying, pulverizes and uses 90 DEG C of wet heating to process 60min; In Geotrichum (Galactomyces candidum) after activation, add without bacterial nutrient solution, with transfer needle, lawn is washed down, then accessed in the seed nutrient solution of sterilization treatment, shaking table is cultivated 24h (180r/min, 25 DEG C); Seed culture fluid is inoculated in the Suillus albidipes (Peck) Sing powder after sterilizing and is fermented in the ratio of 2:3,25 DEG C of fermentation temperatures, fermentation time 6h; Tunning is the natural perfume material that is derived from bolete of the present invention.By the ethanol water refluxing extraction of 75wt% three times for this tunning, each 1.5h, merge extract concentrated, refining through the ethanol water back dissolving of 95wt% again, remove insoluble matter, obtain natural perfume material, this natural perfume material added in electronic cigarette tobacco tar according to 0.15% ratio, give electronic cigarette flue gas alcohol and the fragrant note of wine.Fragrance alcohol and exquisiteness, stimulation reduces, the damp property improvement of flue gas, flue gas is soft, and pleasant impression is comfortable.Meanwhile, detect and find, the burst size of four kinds of peculiar N-nitrosamine in flue gas has had obvious reduction equally.
Embodiment 3:
Boletus aereus (Boletus aereus) fructification low temperature drying, pulverizes and uses 110 DEG C of wet heating to process 40min; In Geotrichum (Galactomyces candidum) bacterial strain after activation, add without bacterial nutrient solution, lawn is washed down with transfer needle, then accessed in the seed nutrient solution of sterilization treatment, shaking table is cultivated 12h (180r/min, 28 DEG C); Seed culture fluid is inoculated in sterilizing Suillus albidipes (Peck) Sing powder and is fermented in the ratio of 3:2,25 DEG C of fermentation temperatures, fermentation time 8h; Tunning is the natural perfume material that is derived from bolete of the present invention.To the ethanol water refluxing extraction three times that adds 75wt% in this tunning, each 1.5h, merge extract concentrated, more refining through straight alcohol back dissolving, remove insoluble matter, obtain natural perfume material, this natural perfume material is added in quid (smokeless tobacco) according to 0.01% ratio, make fragrant sensation obviously, pleasant impression is comfortable, harmony increases, excitant decrease to some degree.
Claims (7)
1. a preparation method who comes from the natural perfume material of bolete, comprises the following steps: bolete fructification is carried out to sterilization treatment, then after adding Geotrichum (Galactomyces candidum) to ferment, obtain described natural perfume material.
2. according to the preparation method of claim 1, wherein said bolete is selected from the edible bolete in Boletaceae (Boletaceae) and pinecone Boletaceae (Strobilomycetaceae).
3. according to the preparation method of claim 1, wherein said bolete is selected from Suillus albidipes (Peck) Sing (Boletus edulis), Boletus luridus (Boletus Luridus) or Boletus aereus (Boletus aereus).
4. according to the preparation method of claim 1, the process conditions of described fermentation are as follows: 20 DEG C～35 DEG C of fermentation temperatures, fermentation time 6～12h.
5. according to the preparation method of claim 1, wherein said natural perfume material further, through solvent extraction and concentrated, obtains natural perfume material concentrate.
6. the natural perfume material that comes from bolete of preparing according to the preparation method of claim 1 is for giving the purposes of tobacco product flavouring.
7. the natural perfume material that comes from bolete of preparing according to the preparation method of claim 1 is for reducing the purposes of burst size of lighting the peculiar N-nitrosamine of tobacco in the flue gas of type cigarette, electronic cigarette or electric heating type cigarette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410344593.3A CN104126868B (en) | 2014-07-18 | 2014-07-18 | Come from the preparation method of the natural perfume material of bolete and to the purposes of fragrance-enhancing tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410344593.3A CN104126868B (en) | 2014-07-18 | 2014-07-18 | Come from the preparation method of the natural perfume material of bolete and to the purposes of fragrance-enhancing tobacco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104126868A true CN104126868A (en) | 2014-11-05 |
| CN104126868B CN104126868B (en) | 2016-08-24 |
Family
ID=51799919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410344593.3A Active CN104126868B (en) | 2014-07-18 | 2014-07-18 | Come from the preparation method of the natural perfume material of bolete and to the purposes of fragrance-enhancing tobacco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104126868B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5372149A (en) * | 1992-03-25 | 1994-12-13 | Roth; David S. | Sterilization process in the manufacturing of snuff |
| CN101638608A (en) * | 2009-08-28 | 2010-02-03 | 湖南先伟实业有限公司 | Tobacco fig flavor prepared by microbes as well as preparation method and application thereof |
| CN102002428A (en) * | 2009-09-02 | 2011-04-06 | 湖北中烟工业有限责任公司 | Method for extracting volatile oil from geotrichum candidum through fermentation of burdock by utilizing supercritical extraction method and application |
| CN102068032A (en) * | 2010-12-08 | 2011-05-25 | 无锡华海香料有限公司 | Method for preparing cigarette perfume by fermenting aromatic tobacco seeds with fungal geotrichum candidum |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN102920016A (en) * | 2012-11-09 | 2013-02-13 | 成都宏亿实业集团有限公司 | Porcini extract and preparation method and application thereof |
| CN103013666A (en) * | 2012-12-14 | 2013-04-03 | 湖北中烟工业有限责任公司 | Method for preparing corn extract paste for fermentation type cigarette |
| CN103099303A (en) * | 2013-02-04 | 2013-05-15 | 红云红河烟草(集团)有限责任公司 | Application of leavening japonica rice in improving quality of cigarette flavor |
-
2014
- 2014-07-18 CN CN201410344593.3A patent/CN104126868B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5372149A (en) * | 1992-03-25 | 1994-12-13 | Roth; David S. | Sterilization process in the manufacturing of snuff |
| CN101638608A (en) * | 2009-08-28 | 2010-02-03 | 湖南先伟实业有限公司 | Tobacco fig flavor prepared by microbes as well as preparation method and application thereof |
| CN102002428A (en) * | 2009-09-02 | 2011-04-06 | 湖北中烟工业有限责任公司 | Method for extracting volatile oil from geotrichum candidum through fermentation of burdock by utilizing supercritical extraction method and application |
| CN102068032A (en) * | 2010-12-08 | 2011-05-25 | 无锡华海香料有限公司 | Method for preparing cigarette perfume by fermenting aromatic tobacco seeds with fungal geotrichum candidum |
| CN102119784A (en) * | 2011-02-23 | 2011-07-13 | 红云红河烟草(集团)有限责任公司 | Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof |
| CN102920016A (en) * | 2012-11-09 | 2013-02-13 | 成都宏亿实业集团有限公司 | Porcini extract and preparation method and application thereof |
| CN103013666A (en) * | 2012-12-14 | 2013-04-03 | 湖北中烟工业有限责任公司 | Method for preparing corn extract paste for fermentation type cigarette |
| CN103099303A (en) * | 2013-02-04 | 2013-05-15 | 红云红河烟草(集团)有限责任公司 | Application of leavening japonica rice in improving quality of cigarette flavor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104126868B (en) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102823937A (en) | Extracting method of submerged fermentation mycelium extractive of lepista nuda and application thereof in cigarettes | |
| CN102703217B (en) | Noni extract and application thereof | |
| KR20130128641A (en) | Rice wine using dendropanax morbifera lev. and process for preparing the same | |
| CN103462212A (en) | Method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid | |
| CN110771947A (en) | Spice functional module for cigarette thickening and increasing | |
| CN106148011A (en) | The preparation method of a kind of cigarette radix valerianae extract and the application in Medicated cigarette thereof | |
| CN101637303A (en) | Lemon extract, preparation method thereof and application thereof in cigarette flavoring | |
| CN108618192A (en) | A kind of 15 are formulated electronic cigarette tobacco tar and preparation method thereof | |
| CN103393214B (en) | Aromatic tobacco ethyl alcohol and propylene glycol mixed solution extract and preparation method and application of aromatic tobacco ethyl alcohol and propylene glycol mixed solution extract | |
| CN110358630B (en) | Spice with fresh and spicy flavor for cigarettes, preparation method of spice and application of spice in cigarettes | |
| CN105105319A (en) | Preparation method of maca extracts and application of maca extracts in cigarettes | |
| CN1986759A (en) | Petroleum extracted sophora flower extractum and its application in perfuming cigarette | |
| CN108185512A (en) | A kind of perfume material for the fragrant section of cigarette production of not burning for mixed type heating and preparation method thereof | |
| CN102899167B (en) | Tobacco flavor with uniform and stable fragrance and preparation method thereof | |
| CN115558548B (en) | Rosin-flavored essence for cigarettes and preparation method and application thereof | |
| CN105713924A (en) | Method for preparing fermented type tobacco spice from rosa sterilis | |
| CN103349353A (en) | Novel application of edible fungus | |
| CN104099182B (en) | Come from the purposes of compound spices for reducing the peculiar N-nitrosamine burst size of tobacco in cigarette smoke of bolete | |
| CN109868186A (en) | A method of essence spice for cigarette is prepared using cordyceps sinensis or Jin Er and flower | |
| CN109825371A (en) | Utilize cordyceps sinensis or Jin Er and the method for flower preparation flavors and fragrances | |
| CN104126868A (en) | Preparation method of natural perfume deriving from porcini and tobacco aroma enhancement application thereof | |
| CN110564504A (en) | Cigarette essence containing clausena lansium extract, preparation method and application | |
| CN103271429B (en) | The application of moringa oleifera leaf extractive in cigarette and the cigarette containing moringa oleifera leaf extractive | |
| CN106136298A (en) | A kind of litchi spirit odor type electronic cigarette tobacco tar | |
| CN108451004B (en) | Method for preparing burley tobacco reactant by using fruit extract and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |