CN104120153B - Lecithin application in improving fermentation of plant straws production ethanol production - Google Patents
Lecithin application in improving fermentation of plant straws production ethanol production Download PDFInfo
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- CN104120153B CN104120153B CN201410300598.6A CN201410300598A CN104120153B CN 104120153 B CN104120153 B CN 104120153B CN 201410300598 A CN201410300598 A CN 201410300598A CN 104120153 B CN104120153 B CN 104120153B
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- lecithin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention provides lecithin application in improving fermentation of plant straws production ethanol production.The present invention is by the lecithin adding 30～100mg/L in culture propagation culture medium, other process control condition is the same, the yield producing ethanol for fermenting substrate with straw hydrolysis sugar is made to reach 0.47, ethanol content reaches 14.56% (v/v), and be not added with in the production technology of lecithin, the yield of ethanol is 0.40, and ethanol content is 12.42% (v/v), and lecithin is remarkably improved alcohol fermentation yield.The method increase fermentation of plant straws alcohol getting rate, provide new approaches for fully developing of straw, for reducing environmental pollution, it is ensured that grain security is significant.
Description
Technical field
The present invention relates to field of microbial fermentation, specifically, relate to lecithin in fermenting and producing second
Application and a kind of passing through in alcohol yield add lecithin raising fermentation of plant straws in culture medium
The method producing ethanol production.
Background technology
The most exhausted and the ever-increasing greenhouse gases effect of world petroleum resource, makes people couple
The non-petroleum energy creates great interest.Utilize biomass material to produce alcohol fuel part to substitute
Fossil fuel is own through causing people greatly to pay close attention to.Alcohol fuel is as a kind of regenerative resource, permissible
By glucide fermenting and producing widely, wherein utilize with low cost, the cellulosic of enormous amount
It is the most noticeable that class material, such as straw produce alcohol fuel.
Straw is abundant agricultural wastes, mainly by cellulose, hemicellulose and lignin
Composition.Straw is huge in China's yield, utilizes fermentation of plant straws to produce ethanol and not only alleviates
The series of problems such as the crisis of resource that the mankind are faced, is short of food, environmental pollution, are also the mankind
Sustainable development provides guarantee.
Fermentation of plant straws produces ethanol and typically uses two-step fermentation, the most first plants pretreated
Fibres element raw material is hydrolyzed to reducing sugar, and then the fermentation of recycling reducing sugar obtains ethanol.Plant straw
Mainly containing glucose and xylose in stalk hydrolysis sugar, in different hydrolysis sugars, both ratios have the biggest difference.
Fermentation of plant straws produces ethanol it is crucial that improve the thalline utilization rate to xylose, and then improves ethanol
Yield.
Summary of the invention
The present invention provide a kind of improve fermentation of plant straws produce ethanol production method, the method with
Straw hydrolysis sugar is fermentation substrate, adds lecithin in the fermentation medium, is sent out by yeast
Ferment produces ethanol.
In the inventive method, the final concentration of 30-100mg/L of lecithin in fermentation medium.
Preferably, the final concentration of 50-80mg/L of lecithin in fermentation medium.
It is highly preferred that the final concentration of 60mg/L of lecithin in fermentation medium.
Described straw is corn straw, rape stalk, broomcorn straw, wheat stalk, Caulis et Folium Oryzae
One or more in straw, Caulis Sacchari sinensis.
Described yeast is saccharomyces cerevisiae (Saccharomyces cerevisiae), candida mycoderma (Candida
Albicans), pichia stipitis (Pichia stipitis) or its genetic engineering modified bacterium.
The invention provides lecithin application in improving fermentation of plant straws production ethanol production.
In above-mentioned application, specifically, it is to add final concentration of 30-100mg/L in the fermentation medium
Lecithin.
Preferably, it is the lecithin adding final concentration of 50-80mg/L in the fermentation medium.
It is highly preferred that be the lecithin adding final concentration of 60mg/L in the fermentation medium.
It has also been found that, if add Mn the most simultaneously2+And lecithin, then can enter
One step improves the yield of fermentative production of ethanol.Further, present invention also offers in fermentation culture
Base adds the Mn of final concentration of 0.4-0.8mmol/L simultaneously2+With final concentration of 50-80mg/L's
Lecithin application in the yield improving fermentative production of ethanol.
Preferably, above-mentioned Mn2+Final concentration of 0.6mmol/L.
In one embodiment of the invention, add the most simultaneously final concentration of
0.6mmol/L Mn2+After 60mg/L lecithin, fermentation termination is surveyed ethanol content and is
15.46% (v/v), residual sugar 0.10% (w/v), alcohol getting rate is 0.49.
Fermentation medium of the present invention, containing following components:
Straw hydrolysis sugar is calculated as 16%-30% (w/v), yeast extract with reducing sugar
0.4%-0.7% (w/v), peptone 0.2%-0.5% (w/v), carbamide 0.01%-0.1% (w/v), ovum phosphorus
Fat 30-100mg/L, pH3.5-6.5, temperature 30-32 DEG C.
Further, described fermentation medium, containing following components:
Straw hydrolysis sugar is calculated as 25% (w/v), yeast extract 0.5% (w/v), albumen with reducing sugar
Peptone 0.4% (w/v), carbamide 0.05% (w/v), lecithin 50-80mg/L, pH3.5-6.5, temperature
30-32℃。
Preferably, described fermentation medium, containing following components:
Straw hydrolysis sugar is calculated as 25% (w/v), yeast extract 0.5% (w/v), albumen with reducing sugar
Peptone 0.4% (w/v), carbamide 0.05% (w/v), Mn2+0.4-0.8mmol/L, lecithin 50-80mg/L,
PH3.5-6.5, temperature 30-32 DEG C.
It is highly preferred that the fermentation medium of the present invention contains following components:
Straw hydrolysis sugar is calculated as 25% (w/v), yeast extract 0.5% (w/v), albumen with reducing sugar
Peptone 0.4% (w/v), carbamide 0.05% (w/v), Mn2+0.6mmol/L, lecithin 60mg/L, pH
3.5-6.5, temperature 30-32 DEG C.
The invention provides the application in fermentative production of ethanol of the above-mentioned fermentation medium.
It is an advantage of the current invention that: improve ethanol obtain by adding lecithin in the fermentation medium
Rate and yield, compared with fermentation test result un-added with traditional handicraft so that ethanol content reaches
To 14.56% (v/v), alcohol getting rate reaches 0.47, and is not added with in the fermentation process of lecithin, second
Alcohol content is 12.42% (v/v), and the yield of ethanol is 0.40.The method that the present invention provides is simple,
Improve the yield of fermentation of plant straws ethanol, less energy consumption, reduce the cost of fermentative production of ethanol,
New approaches are provided, for reducing environmental pollution, it is ensured that grain for fully developing of straw
Food safety is significant.
Accompanying drawing explanation
Fig. 1 is variable concentrations lecithin on fermentation of plant straws produce ethanol production affect figure.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.Embodiment
Middle used yeast is saccharomyces cerevisiae, its source: be purchased from Zhejiang University, gene recombinaton saccharomyces cerevisiae
S.cerevisiae ZU-10；Lecithin is purchased from Anqing Zhongchuang Engineering Technology Co., Ltd..Purchase
Buy producer and the feasibility of raw material is simply described, be not limited to the scope of the present invention.
Alcohol getting rate (g ethanol/g glucose)=terminal concentration of alcohol/(initial glucose concentration-
Remaining sugar concentration).Ethanol density is with 0.789g/cm3Meter.
Embodiment 1 lecithin produces the impact of ethanol production to fermentation of plant straws
The cultivation of saccharomyces cerevisiae seed: take inclined-plane Saccharomyces Cerevisiae in S .cerevisiae that a ring is fresh
ZU-10 thalline accesses containing 100mL growth medium (glucose 3% (w/v), yeast leaching cream
0.4% (w/v), peptone 0.3% (w/v).) 250mL shaking flask in, the sealing of eight layers of gauze, then,
On rotary shaker, (rotating speed: 150r/min) can be used for inoculation fermentation training after cultivating 15h in 30 DEG C
Support base.
Shake flask fermentation is tested: by the inoculum concentration of 5% (v/v), cultured seed is accessed fermentation culture
Base, fermentative medium formula is corn straw hydrolysis sugar in terms of reducing sugar 25% (w/v), yeast extract
0.5% (w/v), peptone 0.4% (w/v), carbamide 0.05% (w/v), lecithin is respectively 0,30,
60,80,100mg/L, pH are 4.5.Shaking flask is 500mL (configuration water seal anaerobism plug), fills liquid
Amount is 200mL.After inoculation, on rotary shaker, (rotating speed: 150r/min) trains in 30 DEG C
Support.Fermentation period is 48h.Fermentation termination surveys ethanol content, and experimental result is as shown in Figure 1.Fig. 1
Display, along with being gradually increased of lecithin concentration, ethanol production gradually steps up, when dense for lecithin
When degree is for 60mg/L, ethanol content reaches peak, peak 14.56% (v/v), calculates alcohol getting rate and is
0.47.Afterwards, along with lecithin concentration increases, ethanol content is gradually reduced.In view of producing into
This, it is considered herein that when in culture medium, lecithin interpolation final concentration 30-100mg/L can improve ethanol
Fermentation yield.
The assay method of concentration of alcohol: by gas chromatogram quantitatively (U.S. Agilent6890 series gas phase
Chromatograph, joins hydrogen flame ionization detector, injection port and detector temperature and is respectively 160 DEG C and 230
DEG C, column temperature thermostatic control is 90 DEG C, and employing nitrogen is carrier gas, and n-butyl alcohol is internal standard)
Embodiment 2 culture medium adds Mn simultaneously2+With lecithin, fermentation of plant straws is produced ethanol to produce
The impact of amount
After cultivating seed by the method in specific embodiment 1, by identical inoculation shaking flask inoculation fermentation training
Supporting base, test group fermentative medium formula is corn straw hydrolysis sugar in terms of reducing sugar 25% (w/v),
Yeast extract 0.5% (w/v), peptone 0.4% (w/v), carbamide 0.05% (w/v), Mn2+0.6mmol/L,
Lecithin 60mg/L, pH are 4.5.Simultaneously with without Mn2+And lecithin, remaining formula is identical
As a control group.Shaking flask is 500mL (configuration water seal anaerobism plug), and liquid amount is 200mL.
After inoculation, on rotary shaker, (rotating speed: 150r/min) cultivates in 30 DEG C.Fermentation period
For 48h.It is 12.42% (v/v) that matched group fermentation termination surveys ethanol content, residual sugar 0.5% (w/v), second
Alcohol yield is 0.40；It is 15.46% (v/v) that test group fermentation termination surveys ethanol content, residual sugar
0.10% (w/v), alcohol getting rate is 0.49.Result shows, with the addition of Mn2+Test group with lecithin
Ethanol content and yield be significantly higher than and do not add Mn2+Matched group with lecithin.
Although, the most with a general description of the specific embodiments the present invention has been made detailed
Description, but on the basis of the present invention, it can be made some modifications or improvements, this is to this area
It is apparent from for technical staff.Therefore, done without departing from theon the basis of the spirit of the present invention
These modifications or improvements, belong to the scope of protection of present invention.
Claims (4)
1. one kind is improved the method that fermentation of plant straws produces ethanol production, it is characterised in that
With straw hydrolysis sugar as fermentation substrate, add lecithin in the fermentation medium, pass through ferment
Female fermentative production of ethanol；The final concentration of 30-100mg/L of lecithin in fermentation medium；Fermentation
Culture medium is also added with the Mn of final concentration of 0.4-0.8mmol/L2+；Described yeast is wine brewing ferment
Female (Saccharomyces cerevisiae), pichia stipitis (Pichia stipitis) or its
Genetic engineering modified bacterium.
2. the method for claim 1, it is characterised in that ovum phosphorus in fermentation medium
The final concentration of 50-80mg/L of fat.
3. the method for claim 1, it is characterised in that ovum phosphorus in fermentation medium
The final concentration of 60mg/L of fat.
4. the method for claim 1, it is characterised in that described straw is beautiful
One in rice straw, rape stalk, broomcorn straw, wheat stalk, rice straw, Caulis Sacchari sinensis
Or it is several.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102498122A (en) * | 2009-07-01 | 2012-06-13 | 威斯康星校友研究基金会 | Biomass hydrolysis |
| CN102533894A (en) * | 2011-12-22 | 2012-07-04 | 中山大学 | Method for maintaining stable content of Monacolin K and its acid structure in monascus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498122A (en) * | 2009-07-01 | 2012-06-13 | 威斯康星校友研究基金会 | Biomass hydrolysis |
| CN102533894A (en) * | 2011-12-22 | 2012-07-04 | 中山大学 | Method for maintaining stable content of Monacolin K and its acid structure in monascus |
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