CN104117429B - A kind of heating oscillating magnetic flux separator - Google Patents
A kind of heating oscillating magnetic flux separator Download PDFInfo
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- CN104117429B CN104117429B CN201410347425.XA CN201410347425A CN104117429B CN 104117429 B CN104117429 B CN 104117429B CN 201410347425 A CN201410347425 A CN 201410347425A CN 104117429 B CN104117429 B CN 104117429B
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Abstract
The invention discloses a kind of heating oscillating magnetic flux separator, comprise three primary modules, i.e. Magneto separate module, heating module and vibration module.Magneto separate module includes linear electric motors, leading screw, magnet carrier, permanent magnet, and heating module includes electromagnetism load push-pull, spring, gripper shoe, support column, heating tank, heating film, and vibration module includes vibrating motor, eccentric hammer.This device drives magnet carrier to move on leading screw by linear electric motors, thus drives the up and down motion of permanent magnet, controls permanent magnet and realizes reunion and the dispersion of magnetic nanoparticle with the relative position of microwell plate；Promoting gripper shoe up and down motion by electromagnetism load push-pull, and then drive heating tank to move up and down by support column, the distance controlling heating tank and microwell plate hole wall realizes heating and the function closed.Finally achieve biological specimen processing means based on Magneto separate that is fully functional, small and exquisite and that be easily integrated, utilize robot arm device can realize the automation of whole experimentation.
Description
Technical field
The present invention relates to biological specimen treatment technology, particularly a kind of high flux full automatic heating oscillating magnetic flux separator.
Background technology
Biological specimen is varied, has blood plasma, serum, whole blood, lymph, saliva, various tissue, hair, urine, bile, tear, spinal fluid, sweat, milk, amniotic fluid, ight soil and the gas of exhalation.Biological specimen is studied the pre-treatment work first having to carry out biological specimen, typically require and disposably great amount of samples is carried out operation rapidly and efficiently, therefore, suitably biological specimen process and analytical equipment can alleviate the workload of researcher significantly, operating efficiency is provided, preferably advances the development of subject.
Along with developing rapidly of nanometer science and technology, nano material is gradually applied to life science, and the research and development for life science provides new technology and means.Wherein, the preparation and application of magnetic nanoparticle have become study hotspot.Magnetic nanoparticle has that specific surface area is big, superparamagnetism, be prone to the advantages such as surface modification, is widely used in biomedical sector, and wherein, it is one of its important application in this field that magnetic nanoparticle separates for biological specimen.During magnetic nanoparticle separation of biomolecules, have that separating rate is fast, efficiency is high, reusable, simple to operate, easily realize functionalization, easily realize automation and do not affect the feature such as special physicochemical properties and biocompatibility such as activity of separate substance.Further, use magnetic nanoparticle advantage on Aulomatizeted Detect the most prominent: traditional separation method such as phenol-chloroform method, Chelexl00 method, salting out method etc., there is liquid-liquid separation, the step such as centrifugal, centrifugation apparatus is bulky, complex operation, required time is long, is not suitable for being applied to the demand of Aulomatizeted Detect.And compared with above method, using magnetic nanoparticle lock out operation simple, required time is short, when externally-applied magnetic field, magnetic nanoparticle is magnetized, and is attracted on magnetic pole, when magnetic field disappears, the magnetic on magnetic nanoparticle disappears the most simultaneously, is again dispersed in reactant liquor.Separation and the location etc. that utilize magnetic nanoparticle to carry out biological specimen operate, and are more easy to realize integrated and automation.
Magnetic nanoparticle is widely used in the fixing of the separation of cell, immunoassays, protein and enzyme and the detection etc. of DNA, biological specimen processing method based on Magneto separate generally comprises steps such as adding sample, cell cracking, adsorption of DNA, Magneto separate, heating mixing, corresponding self-reacting device needs have sample-adding, Magneto separate, heat and the function such as vibration, comprises mechanical arm, static-magnetic separator, heater, vibration evenly mixing device and other some functional devices.At least corresponding station of each device, the process that biological specimen to be completed processes, mechanical arm needs to complete microwell plate transfer work between above-mentioned multiple stations repeated multiple timesly, substantially increases workload, bring inconvenience to experiment, considerably increase the possibility of cross pollution simultaneously.Further, this quasi-instrument be unfavorable for small modular and be integrated into large-scale one Automation workstation in.
Summary of the invention
Technical problem: for the deficiencies in the prior art, the invention provides a kind of full automatic heating oscillating magnetic flux separator, magnetic separating device, temperature control equipment and vibration evenly mixing device are combined into one, microwell plate bracket and permanent magnet, heating arrangements, oscillating mechanism are provided, Magneto separate, the function heating or vibrating can be conveniently realized, possess integrality, compact, the advantage that is advantageously integrated.Experimentation can complete without mobile microwell plate the process of biological specimen, solve that transfer microwell plate is brought repeatedly complex operation, cross pollution probability be high, the defect of poor controllability, and be integrated into shortcoming bulky in Automation workstation.
Technical scheme: above-mentioned technical problem, the invention provides a kind of heating oscillating magnetic flux separator, and this device includes three primary modules, i.e. Magneto separate module, heating module and vibration module, and auxiliary body；
Magneto separate module includes linear electric motors, leading screw, magnet carrier, permanent magnet；Heating module includes electromagnetism load push-pull, gripper shoe, spring, support column, heating tank, heating film；Vibration module includes vibrating motor, eccentric hammer；Auxiliary body comprises base, fixed plate, 4 elastic pillars, main frames；
Described base is groove-like, and centre is provided with three fixing grooves, is respectively used to fixing linear electric motors, electromagnetism load push-pull and vibrating motor, and middle two lateral processes place two pieces of fixed plates；Described fixed plate corner is provided with 4 elastic pillars and fixes groove, and described elastic pillar is placed in vertical in fixing groove；
Described main frame is placed on 4 elastic pillars, and described main frame center lower end connects an eccentric hammer, and eccentric hammer is connected with vibrating motor；
Minor face both sides, described main frame top are two fixing terminals, the described built-in ball of fixing terminal, are used for fixing microwell plate so that microwell plate is in same position all the time；Described fixing terminal lays respectively at two minor faces of microwell plate, thus does not affect the mechanical arm crawl to microwell plate；
Described leading screw one end is fixed on linear electric motors, in vertically placing, magnet carrier is connected on described leading screw, magnet carrier stretches out the support of a cuboid, and support is provided with 4 magnetic patch holes, places 4 pieces of permanent magnets, permanent magnet is cuboid, it is placed in parallel between permanent magnet, in the most corresponding 4 pieces of permanent magnets between pipe array adjacent on microwell plate, it is achieved magnetic nanoparticle is in the reunion of sidewall；
Fixing a gripper shoe on described electromagnetism load push-pull, in gripper shoe, 2 support columns are fixed in both sides, and 2 support columns are positioned on same level line and are placed in parallel, and described spring is positioned at lower end in the middle of gripper shoe, is in the middle of two support columns, and heating tank is fixed on support column top；
Described heating tank is an irregular cuboid, is smooth heating surface outside two long limit, places a heating film respectively；Heating tank has 8 contact surfaces, the every a line in one_to_one corresponding microwell plate pipe array, and contact surface is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, it is achieved seamless applying with hole wall；Heating tank top is the via of adaptive microwell plate, makes microwell plate can put in heating tank and fits with contact surface；Aperture on the aperture correspondence microwell plate on heating tank top, can accelerate heat transfer.
Preferably, the contact surface in described heating tank is in the different pipe arrays into microwell plate from described permanent magnet, and both do not collide.
Beneficial effect:
(1) instant invention overcomes existing biological specimen based on Magneto separate and process the deficiency of single device simple function in automation equipment, magnetic separating device, heater and vibrating device are integrated in a device, fully ensure that the incubation with heat under the Magneto separate of solution in microwell plate, fully mixing and uniform temperature, ensure the integrality of function.
(2) present invention is in the whole course of work, microwell plate containing reaction solution is the most static to be fixed on above main frame, the operation that single device achieves Magneto separate, heats and vibrate, the most both save working space and make the effect that device is small and exquisite, ensure that biological specimen processes, the interference between microporous pipe can be avoided again, significantly prevent cross pollution；
(3) in modularized design, this device, Magneto separate, heating and vibration module all can be independently operated, and not interfere with each other；
(4) practical, this device is Open architecture, have that frame for movement is simple, be skillfully constructed and the feature such as be easily achieved, can be integrated on the devices such as large-scale biochemical analyzer, can also independently use as the biological specimen processing means minimized, such device meets the actual demand of user.
Accompanying drawing explanation
Fig. 1 is the monnolithic case figure of the present invention；
Fig. 2 is the component front view of the present invention；
Fig. 3 is the component rearview of the present invention；
Fig. 4 is the bottom schematic view of the present invention；
Fig. 5 is the magnet carrier schematic diagram of the present invention；
Fig. 6 is the heating tank schematic diagram of the present invention；
In figure: base 101, electromagnetism load push-pull 102, fixed plate 103, elastic pillar 104, main frame 105, ball 106, fixing terminal 107, heating tank 108, heating film 109, vibrating motor 201, linear electric motors 202, leading screw 203, magnet carrier 204, permanent magnet 205, gripper shoe 206, support column 208, eccentric hammer 301, heating surface 501, contact surface 502, via 503, aperture 504.
Detailed description of the invention
Below in conjunction with the accompanying drawings and patent of the present invention is described in further detail by embodiment:
A kind of heating oscillating magnetic flux separator, comprises three primary modules, i.e. Magneto separate module, heating module and vibration module.Magneto separate module includes linear electric motors, leading screw, magnet carrier, permanent magnet, and heating module includes electromagnetism load push-pull, spring, gripper shoe, support column, heating tank, heating film, and vibration module includes vibrating motor, eccentric hammer.It addition, also comprise base, fixed plate, the elastic supplementary structure such as pillar and main frame.
Described base is groove-like, and mid portion fixes linear electric motors, electromagnetism load push-pull and vibrating motor, and middle two lateral processes place two pieces of fixed plates.
Described fixed plate corner is provided with 4 elastic pillars and fixes groove, and described elastic pillar is placed in vertical in fixing groove；
Described main frame is placed on 4 elastic pillars, and described main frame center lower end connects an eccentric hammer, and eccentric hammer is connected with vibrating motor；Minor face both sides, described main frame top are two fixing terminals, the described built-in ball of fixing terminal, are used for fixing microwell plate so that microwell plate is in same position all the time.Described fixing terminal lays respectively at two minor faces of microwell plate, thus does not affect the mechanical arm crawl to microwell plate；
Described leading screw one end is fixed on linear electric motors, in vertically placing, described leading screw connects magnet carrier, magnet carrier is provided with the draw-in groove of 4 permanent magnets, places 4 block permanent magnets, and permanent magnet is cuboid, it is placed in parallel between permanent magnet, between pipe array adjacent on microwell plate, a permanent magnet can be placed；
Fixing a gripper shoe on described electromagnetism load push-pull, in gripper shoe, 2 support columns are fixed in both sides, and 2 support columns are positioned on same level line and are placed in parallel, and described spring is positioned at lower end in the middle of gripper shoe, is in the middle of two support columns, and heating tank is fixed on support column top.Described heating tank is an irregular cuboid, is smooth heating surface outside its two long limit, places a heating film respectively；Heating tank has 8 contact surfaces, the every a line in one_to_one corresponding microwell plate array, and contact surface is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, it is achieved seamless applying with hole wall.Heating tank top is the via of adaptive microwell plate, makes microwell plate can put in heating tank and fits with contact surface；Aperture on the aperture correspondence microwell plate on heating tank top, can accelerate heat transfer.
Assembly of the invention, drives magnet carrier to move on leading screw by linear electric motors, thus drives the up and down motion of permanent magnet, controls permanent magnet and realizes reunion and the dispersion of magnetic nanoparticle with the relative position of microwell plate hole wall；Promoting gripper shoe to run up and down by electromagnetism load push-pull, and then drive heating tank to move up and down by support column, control heating tank realizes heating and the function closed with the distance of microwell plate hole wall；Controlling eccentric hammer by vibrating motor drives main frame to realize the vibrating function of whole device.Finally, by control Magneto separate, vibrate, the different sequential that heat can complete the automation mechanized operation of whole nucleic acid extraction.
Further, in the present invention, after the energising of electromagnetism load push-pull, produce magnetic field, drive gripper shoe to move upward so that heating tank and microwell plate hole wall patch and, heating tank is metal material (gold, silver, aluminium, iron etc.), the heating film energising of both sides, utilizes metal heat-conducting principle to realize the temperature conduction of heating tank, it is achieved the heating function of microwell plate.After electromagnetism load push-pull power-off, magnetic field disappears, and drives gripper shoe to move downward, generally 3mm, and heating tank separates with microwell plate, heats film power-off, closes heating function.
Further, in the present invention, the described spring of heating module plays and supports and the effect of buffering heating tank, high vibration when preventing electromagnetism load push-pull from moving up and down.
Further, in the present invention, described elastic pillar plays the fixing and effect of buffering.Vibrating motor energising rear drive eccentric hammer drives main frame of reciprocating vibration, thus drive is fixed on main frame top and fixes the vibration of the microwell plate on terminal, stops vibration after closing vibrating motor.
Further, in the present invention, linear electric motors drive leading screw to drive magnet carrier to move upward so that 4 block permanent magnets in magnet carrier are between the every two row hole walls of microwell plate array, it is achieved the reunion of magnetic nanoparticle；When magnet carrier moves downward, 4 block permanent magnets are positioned at microwell plate array lower end, and magnetic nanoparticle is lost suction-operated.
Further, in the present invention, the contact surface in heating tank is in the different arrays into microwell plate from described permanent magnet, and both do not collide.
Further, in the present invention, the control sequential of magnetic field, heating and vibrating function has following 6 kinds: 1) without magnetic field, without heating, friction；2) without magnetic field, there is heating, have vibration；3) there is magnetic field, without heating, friction；4) without magnetic field, nothing heating, there is vibration；5) there is magnetic field, without heating, friction；6) without magnetic field, there are heating, friction.
As shown in Fig. 1 to Fig. 5, a kind of heating vibration magnetic separating device of the present invention includes base 101, described base 101 is an irregular groove, centre is provided with three fixing grooves, fixing linear electric motors 202, electromagnetism load push-pull 102 and vibrating motor 201, middle both sides are the cuboid of projection, place two pieces of fixed plates 103.The elastic pillar 104 of the Corner Strapped of described fixed plate 103 4, described elastic pillar 104 be cylinder, in vertically placing in fixing groove, infringement to instrument during in order to prevent from vibrating, play cushioning effect.The top of elastic pillar 104 is provided with fixing groove, for fixing and supportive body frame 105.Described ball 106 is fixed on described fixing terminal 107, and described fixing terminal 107 is positioned at the both sides, top of main frame 105, the built-in described ball 106 of described fixing terminal 107, is used for fixing microwell plate, makes microwell plate be in same position all the time.Described fixing terminal 107 lays respectively at two minor faces of microwell plate, thus does not affect the mechanical gripper crawl to microwell plate.Described main frame 105 center lower end connects an eccentric hammer 301, eccentric hammer 301 is connected with vibrating motor 201, vibrating motor 201 is energized, and to drive main frame 105 to realize of reciprocating vibration for rear drive eccentric hammer 301, thus drive and be fixed on main frame 105 top and fix the vibration of microwell plate at terminal 107.nullDescribed leading screw 203 one end is fixed on linear electric motors 202，In vertically placing，Magnet carrier 204 is connected on described leading screw，Magnet carrier 204 stretches out the support of a cuboid，Support is provided with 4 magnetic patch holes，Place 4 block permanent magnets 205，Permanent magnet 205 is in cuboid，It is placed in parallel between permanent magnet 205，The most common microwell plate divides according to different models 6 holes、12 holes、24 holes、48 holes、96 holes、384 holes and 1536 holes etc.，Hole on microwell plate is according to array ordered arrangement，It is provided with tubular container in each hole，I.e. form pipe array，Certain intervals is there is between the pipe array of adjacent two row，Identical interval between the column and the column, direction is the most parallel，Therefore，Each permanent magnet 205 is all stuck in two pipe array spacings，Realize magnetic-particle in the tubular container of both sides in the reunion of sidewall and dispersion.A gripper shoe 206 is fixed on described electromagnetism load push-pull 102, in gripper shoe 206,2 support columns 208 are fixed in both sides, 2 support columns 208 are positioned on same level line and are placed in parallel, described spring 207 is positioned at the middle lower end of gripper shoe 206, being in the middle of two support columns 208, heating tank 108 is fixed on support column 208 top.Described heating tank 108 is an irregular cuboid, is smooth heating surface 501 outside its both sides, places a heating film 109 respectively；Heating tank 108 has eight contact surfaces 502, the every a line in one_to_one corresponding microwell plate array, and contact surface 502 is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, it is achieved seamless applying with hole wall.Heating tank 108 top is the via 503 of adaptive microwell plate, makes microwell plate can put in heating tank 108 and fits with contact surface 502；Aperture on the corresponding microwell plate of the aperture 504 on heating tank 108 top, can accelerate heat transfer.
As a example by nucleic acid extraction process based on Magneto separate, the concrete course of work of this device is as follows:
1, work is initial, is stuck in by microwell plate between two balls 106, places stable, fills the solution that the sample of nucleic acid extraction mixes with lysate in microwell plate.Now device is in init state, magnet carrier 204 and gripper shoe 206 and is all located at the minimum point of respective vertical direction.Solution in microwell plate is not affected by the magnetic field of permanent magnet 205, and heating tank 108 departs from the tube wall in microwell plate array, and electromagnetism load push-pull 102, vibrating motor 201 and linear electric motors 202 are all in off working state.
2, timed incubations.It is energized to electromagnetism load push-pull 102, produces magnetic field, drive gripper shoe 206 to move upward, make heating tank 108 and microwell plate hole wall patch and, it is energized then to the heating film 109 of both sides, utilizes metal heat-conducting principle to realize the temperature conduction of heating tank 108, it is achieved the heating function of microwell plate.After certain time, electromagnetism load push-pull power-off, now magnetic field disappears, and drives gripper shoe to move downward about 3mm, and heating tank separates with microwell plate, heats film 109 power-off, closes heating function.
3, vibration mixing.Choose whether to open vibrating function according to experiment demand.Vibrating motor 201 can be opened while heating, drive eccentric hammer 301 to drive main frame 105 to vibrate, thus realize the mixing of solution in microwell plate.Vibrating motor is closed while closing heating.
4, Magneto separate.Magnetic particle solution with the sample specific bond of nucleic acid extraction is added in microwell plate, opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward, permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, now the magnetic-particle in solution carries the material to be separated of combination and is adsorbed to sidewall, achieving sidewall to reunite, remaining liq can siphon away by modes such as pipettors, can realize target substance and separate with non-targeted material.
5, release magnetic nanoparticle.Linear electric motors 202 antiport drives leading screw 203 to drive magnet carrier 204 to move downward, and makes permanent magnet 205 rest in microwell plate bottom part down, and now the magnetic of permanent magnet 205 loses suction-operated to the solution in microwell plate.
6, vibration mixing, adds cleaning fluid in microwell plate, opens vibrating motor 201, drives eccentric hammer 301 to drive main frame 105 to vibrate, it is achieved the mixing again of solution of magnetic nanoparticles in microwell plate.Close vibrating motor.
7, complete after repeating many times of step 4-6 to clean, eluent is added in microwell plate.
8, Magneto separate.Opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward,
Permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, now the magnetic-particle in solution carries the material to be separated of combination and is adsorbed to sidewall, achieve sidewall to reunite, remaining liq is shifted by the modes such as available pipettor, and the liquid being transferred out is the material of extraction.
The conversion of above-mentioned several duty can be realized by control system, note controlling sequential, in conjunction with liquid-transfering device, the experiment of automatic biological sample process can be realized.
The above is only the preferred embodiment of the present invention; it is noted that, for those skilled in the art; under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (2)
1. a heating oscillating magnetic flux separator, it is characterised in that: this device includes three primary modules, i.e. magnetic
Separation module, heating module and vibration module, and auxiliary body；
Magneto separate module includes linear electric motors (202), leading screw (203), magnet carrier (204), permanent magnet (205)；
Heating module include electromagnetism load push-pull (102), gripper shoe (206), spring (207), support column (208),
Heating tank (108), heating film (109)；Vibration module includes vibrating motor (201), eccentric hammer (301)；
Auxiliary body comprises base (101), fixed plate (103), 4 elastic pillars (104), main frames (105)；
Described base (101) is in groove-like, and centre is provided with three fixing grooves, is respectively used to fixing linear electric motors
(202), electromagnetism load push-pull (102) and vibrating motor (201), middle two lateral processes two pieces of fixed plates of placement
(103)；Described fixed plate (103) corner is provided with 4 elastic pillars and fixes groove, described elastic pillar (104)
In vertically placing in fixing groove；
Described main frame (105) is placed on 4 elastic pillars (104), in described main frame (105)
Heart lower end connects an eccentric hammer (301), and eccentric hammer (301) is connected with vibrating motor (201)；
Minor face both sides, described main frame (105) top are two fixing terminals (107), described fixing terminal (107)
Built-in ball (106), is used for fixing microwell plate so that microwell plate is in same position all the time；Described fixing end
Son (107) lays respectively at two minor faces of microwell plate, thus does not affect the mechanical arm crawl to microwell plate；
Described leading screw (203) one end is fixed on linear electric motors (202), in vertically placing, on described leading screw
Connecting magnet carrier (204), magnet carrier (204) stretches out the support of a cuboid, and support sets
Have 4 magnetic patch holes, place 4 pieces of permanent magnets (205), permanent magnet (205) in cuboid, permanent magnet (205)
Between be placed in parallel, between pipe array adjacent on microwell plate respectively in corresponding 4 pieces of permanent magnets (205) one
Individual, it is achieved magnetic nanoparticle is in the reunion of sidewall；
Fixing a gripper shoe (206) on described electromagnetism load push-pull (102), the upper both sides of gripper shoe (206) are solid
Fixed 2 support columns (208), 2 support columns (208) are positioned on same level line and are placed in parallel, described bullet
Spring (207) is positioned at lower end in the middle of gripper shoe (206), is in the middle of two support columns (208), support column
(208) heating tank (108) is fixed on top；
Described heating tank (108) is an irregular cuboid, is smooth heating outside two long limit
Face (501), places heating film (109) respectively；Heating tank has 8 contact surfaces (502), one_to_one corresponding
Every a line in microwell plate pipe array, contact surface (502) is the structure of multiple taper surface, the tapering of taper surface
Mate with the tapering of tubular container, it is achieved seamless applying with hole wall；Heating tank top is the mistake of adaptive microwell plate
Hole (503), makes microwell plate can put in heating tank and fits with contact surface (502)；Heating tank top little
Aperture on the corresponding microwell plate in hole (504), can accelerate heat transfer.
Heating oscillating magnetic flux separator the most according to claim 1, it is characterised in that: described heating tank
(108) contact surface (502) in is in the different pipe arrays into microwell plate from described permanent magnet (205),
Both do not collide.
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| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN109364804A (en) * | 2018-09-25 | 2019-02-22 | 珠海丽珠试剂股份有限公司 | A kind of incubation oscillation device |
| CN109269873A (en) * | 2018-10-10 | 2019-01-25 | 东南大学 | Magneto separate heating device for the processing of high-throughput biological sample |
| CN109504600A (en) * | 2018-12-11 | 2019-03-22 | 东南大学 | A kind of constant temperature oscillation incubating device suitable for liquor removing workstation |
| CN111811913A (en) * | 2019-04-10 | 2020-10-23 | 中国水产科学研究院 | Full-automatic sample oscillation extraction separation device and method based on magnetic separation |
| CN114713367B (en) * | 2022-03-16 | 2023-07-18 | 青岛瑞斯凯尔生物科技有限公司 | Oscillating magnetic separation device and method for flow type pre-sample processor |
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| CN103861735A (en) * | 2014-03-19 | 2014-06-18 | 东南大学 | Magnetic separation device |
| CN204018008U (en) * | 2014-07-18 | 2014-12-17 | 东南大学 | A kind of heating oscillating magnetic flux separator |
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| US6672458B2 (en) * | 2000-05-19 | 2004-01-06 | Becton, Dickinson And Company | System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample |
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| US6117398A (en) * | 1995-04-01 | 2000-09-12 | Roche Diagnostics Gmbh | System for releasing and isolating nucleic acids |
| CN102596412A (en) * | 2009-10-16 | 2012-07-18 | 普罗梅加公司 | Heating, shaking, and magnetizing apparatus and method of operating the same |
| CN103861735A (en) * | 2014-03-19 | 2014-06-18 | 东南大学 | Magnetic separation device |
| CN204018008U (en) * | 2014-07-18 | 2014-12-17 | 东南大学 | A kind of heating oscillating magnetic flux separator |
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