CN104109697B - The method of citric acid wastewater backflow fermentation production of citric acid - Google Patents
The method of citric acid wastewater backflow fermentation production of citric acid Download PDFInfo
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Abstract
The method of citric acid wastewater backflow fermentation production of citric acid: (1) starchy material is mixed homogeneously with citric acid wastewater, adds high-temperatureα-amylase; (2) by mixed liquor through twice injection, qualified the mixed liquid that liquefies is obtained through iodine examination; Again mixed for liquefaction liquid is obtained liquefaction clear liquid through filter press; (3) being mixed with the mixed liquid of liquefaction by liquefaction clear liquid, addition citric acid wastewater carries out moisturizing and obtains seed culture medium, carries out the seed culture of strain; (4) being mixed with the mixed liquid of liquefaction by liquefaction clear liquid, addition citric acid wastewater carries out moisturizing and obtains fermented nutritive liquid, carries out fermentation culture; (5) removing thalline through solid-liquid separation after fermentation ends, extract through hydrogen calcium method or chromatography and obtain citric acid, the waste water of generation returns to step (1) and step (3), (4), so circulates. This method fermentation stability and suitable with former technology level, has saved process water and steam consumption, has saved cost of sewage disposal, has important environmental benefit, economic benefit.
Description
Technical field
The present invention relates to fermentation engineering field, especially relate to a kind of citric acid wastewater without any process, the method for the fermentation production of citric acid that directly refluxes.
Background technology
Citric acid has the feature of the tart flavour of pleasant, entrance tart flavour frank, without issue, safety non-toxic because of it, so being widely used in the fields such as food, medicine and chemical industry, it has also become growing amount and the maximum edible organic acid of consumption figure on our times. China is citric acid exported country maximum in the world, and the production capacity of 2012 alreadys more than l10 ten thousand tons, and the citric acid of about 90% exports to foreign countries, and accounts for Gross World Product about 70%. Citric Acid Production mainly adopts liquid submerged fermentation, can produce substantial amounts of waste water, wherein contain the materials such as some organic acid, sugar, protein colloid, mineral in extraction process, and COD is up to 350kg/t citric acid, at concentrations up to 10000 ~ 15000ppm. If these waste water are not treated or deal with improperly, not only cause serious environmental pollution, also result in the waste of great lot of water resources, add enterprise's production cost simultaneously. Therefore how effectively to process the high concentrated organic wastewater that citric acid fermentation produces, be citric acid industry problem demanding prompt solution.
The main methods of existing citric acid wastewater: anaerobic biological process, aerobe method, anaerobic-aerobic combined method, they are to utilize the Organic substance in aerobic or anaerobe degradation of sewage under appropriate conditions, partial organic substances is converted into own cells composition simultaneously, thus reducing the COD of waste water. From current citric acid industry wastewater treatment present situation, effect is unsatisfactory, and these methods are it is generally required to early stage pretreatment, and the cycle is longer, and waste water treatment is not thorough, it is necessary to advanced treating further, can be only achieved discharge standard, and processing cost is higher. Therefore, how effectively to realize energy-saving and emission-reduction, reduce cost, be the encountered key subjects of citric acid industry healthy development. If citric acid wastewater can reuse after direct reuse or simple process, significant for solving a sewage disposal difficult problem.
Summary of the invention
For the problems referred to above that prior art exists, the applicant provides the method for a kind of citric acid wastewater backflow fermentation production of citric acid.This method fermentation stability and suitable with former technology level, has saved process water and steam consumption, has saved cost of sewage disposal, has important environmental benefit, economic benefit.
Technical scheme is as follows:
The method of citric acid wastewater backflow fermentation production of citric acid, comprises the steps:
(1) starchy material and citric acid wastewater are when 50 ~ 60 DEG C, according to the ratio mix homogeneously of 1:1.5 ~ 1:4 in material-compound tank, regulating the pH to 5.8 ~ 6.0 of mixed liquor with calcium hydroxide, be subsequently adding high-temperatureα-amylase, its addition is 15 ~ 40U/g Semen Maydis powder;
(2) step (1) gained mixed liquor being carried out two-step pretreatment: first by mixed liquor through twice injection, one time injection temperation is 97 ~ 101 DEG C, and secondary injection temperature is 120 ~ 130 DEG C, be light brown and qualified through iodine examination, obtain liquefying mixed liquid; Again by mixed for the liquefaction of 60 ~ 80% liquid through filter press, obtain liquefaction clear liquid;
(3) clear liquid that liquefied by step (2) gained mixes in the ratio of 1:1.5 ~ 1:2.5 with the mixed liquid of liquefaction, and add citric acid wastewater and carry out moisturizing so that mixed liquid total sugar is 10 ~ 12%, and total nitrogen is 0.19 ~ 0.40%, obtain seed culture medium, carry out the seed culture of strain;
(4) clear liquid that liquefied by step (2) gained mixes in the ratio of 3.5:1 ~ 5:1 with the mixed liquid of liquefaction, and add citric acid wastewater and carry out moisturizing, making mixed liquid total sugar is 14 ~ 17%, total nitrogen is 0.05 ~ 0.15%, obtain fermented nutritive liquid, step (3) seed culture gained seed liquor is added fermented nutritive liquid, carries out fermentation culture;
(5) removing thalline through solid-liquid separation after step (4) fermentation ends, extract through hydrogen calcium method or chromatography and obtain citric acid, the waste water of generation returns to step (1) and step (3), (4), so circulates.
Starchy material described in step (1) includes at least one of Semen Maydis powder, wheat flour, tapioca starch, sweet potato powder, starch, molasses, Semen Tritici aestivi.
Citric acid wastewater described in step (1) refers to the useless sucrose solution that hydrogen calcium extraction method produces when manufacturing citric acid or the chromatograph residual liquid produced when chromatograph extraction method manufactures citric acid.
Strain described in step (3) is screen the strong adaptability obtained, the aspergillus niger strain that stability is high in citric acid wastewater.
Useful the having the technical effect that of the present invention
When it is an object of the invention to not change existing main body fermentation technology technology and equipment, by adding reflux cycle pipeline, reduce the biological treatment process even eliminating high concentrated organic wastewater, set up the scheme of the citric acid wastewater backflow fermentation production of citric acid of a set of stable operation.
The present invention can make the waste water that citric acid extraction process produces directly substitute industrial water without any process, sizes mixing liquefaction for raw material and prepares culture medium moisturizing; Cultivate the adaptable strain of waste water, switching fermentation; Fermentation ends, fermentation liquor solid-liquid separation obtains fermentation clear liquid, hydrogen calcium method or chromatography and extracts citric acid, and the citric acid wastewater of generation continues to be back to production system, so realizes recycling of waste water.
Adopting this technology lemon acid waste water circulation use 40 batches, saccharic acid conversion ratio is 95.85% ~ 101.35%, fermentation period at 62 ~ 72h, fermentation stability and suitable with former technology level. The present invention can effectively utilize the carbon and nitrogen sources in waste water, and the citric acid wastewater of 50 ~ 60 DEG C of refluxing can agree with feed temperature, has saved process water and steam consumption, has saved cost of sewage disposal, has important environmental benefit, economic benefit.
Accompanying drawing explanation
Fig. 1 is the Technology schematic flow sheet of citric acid wastewater of the present invention backflow fermentation production of citric acid.
Detailed description of the invention
Below in conjunction with accompanying drawing 1, the present invention is specifically described. the source of the aspergillus niger seed in example below is that Yixing-Union Biochemical Co., Ltd. produces, Southern Yangtze University's grain fermentation technology and technology national engineering laboratory bio-separation engineering research room carry out domestication and cultivate. citric acid wastewater (the waste water that citric acid extraction produces, Yixing-Union Biochemical Co., Ltd. provides) liquefying obtains liquefied corn, add a certain amount of ammonium sulfate, preparation culture medium (total sugar 5% ~ 9%, total nitrogen 0.25% ~ 0.45%), inoculated aspergillus niger seed, (35 DEG C are screened through plate isolation, 48h), select neat in edge, protruding, bigger single bacterium colony, single bacterium colony slant culture (35 DEG C, 5d), shaking flask sieves (35 DEG C again, 320rpm, cultivate 72h) select and produce the strain that acid is higher, then cultivation is tamed further, so circulation repeats screening, obtain adaptable strain, it is numbered AT0019.
Embodiment 1
Semen Maydis powder 200kg, lemon acid waste sucrose solution 600kg, mix homogeneously in 50 DEG C of material-compound tanks, adding calcium hydroxide regulates pH to 5.8, adds the Thermostable α-Amylase 250g that enzyme unit alive is 20000U/g. Liquefying through secondary injection, wherein an injection temperation is 97 DEG C, and secondary injection temperature is 130 DEG C, maintains and maintains 0.5 ~ 1h in tank, and iodine examination is light brown and qualified, and obtain liquefying mixed liquid, then by 70% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:1.5, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 3.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 10.7%, total nitrogen 0.24%, aspergillus niger seed culture 27h, pH2.05, acidity 2.05%; Fermentation initial total sugar 16.10%, total nitrogen 0.05%, cultivation temperature 37 DEG C; Fermentation ends acidity is 16.08% (v/v), and residual total sugar is 2.3%, and residual reducing sugar is 0.5%, and conversion ratio is 99.88%, cycle 67h. Fermentation liquor hydrogen calcium method extracts citric acid, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 batches, and citric acid fermentation level is normal.
Embodiment 2
Tapioca starch 100kg, lemon acid waste sucrose solution 150kg, mix homogeneously in 60 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.9, adds the Thermostable α-Amylase 125g that enzyme unit alive is 20000U/g. Liquefying through secondary injection, wherein an injection temperation is 99 DEG C, and secondary injection temperature is 125 DEG C, maintains 0.5 ~ 1h in tank maintaining, and iodine examination is qualified for light brown, and obtain liquefying mixed liquid, then by 60% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:1.5, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 5.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 10%, total nitrogen 0.2%, aspergillus niger seed culture 26h, pH2.06, acidity 2.7%; Fermentation initial total sugar is 14%, total nitrogen 0.08%, cultivation temperature 37 DEG C;Fermentation ends acidity is 14.19% (v/v), and residual total sugar is 2.0%, and residual reducing sugar is 0.5%, and conversion ratio is 101.35%, cycle 60h. Fermentation liquid extracts citric acid through hydrogen calcium method, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 3
Semen Maydis powder 300kg, tapioca starch raw material 200kg, lemon acid waste sucrose solution 2000kg, mix homogeneously in 55 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.9, adds the Thermostable α-Amylase 800g that enzyme unit alive is 20000U/g. Liquefying through secondary injection, wherein an injection temperation is 101 DEG C, and secondary injection temperature is 120 DEG C, maintains 0.5 ~ 1h in tank maintaining, and qualified for light brown through iodine examination, obtain liquefying mixed liquid, then by 80% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:2, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 5.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed initial total sugar 12%, total nitrogen 0.26%, aspergillus niger seed culture 26h, pH1.99, acidity 2.3%; Fermentation medium initial total sugar is 16.2%, total nitrogen 0.11%, cultivation temperature 37 DEG C; Fermentation ends acidity is 16.1% (v/v), and residual total sugar is 2.02%, and residual reducing sugar is 0.51%, and conversion ratio is 99.38%, cycle 72h. Fermentation liquid extracts citric acid through hydrogen calcium method, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 4
Sweet potato powder raw material 120kg, lemon acid waste sucrose solution 320kg, mix homogeneously in 55 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 6.0, add the Thermostable α-Amylase 180g that enzyme unit alive is 20000U/g, liquefying through secondary injection, wherein an injection temperation is 97 DEG C, and secondary injection temperature is 125 DEG C, 0.5 ~ 1h is maintained in maintaining tank, iodine examination is qualified for light brown, and obtain liquefying mixed liquid, then by 65% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:2, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 10.8%, total nitrogen 0.22%, aspergillus niger seed culture 26h, pH2.06, acidity 2.7%; Fermentation initial total sugar is 16.7%, total nitrogen 0.13%, cultivation temperature 37 DEG C; Fermentation ends acidity is 16.61% (v/v), and residual total sugar is 2.0%, and residual reducing sugar is 0.5%, and conversion ratio is 99.5%, cycle 69h. Fermentation liquid extracts citric acid through hydrogen calcium method, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 5
Wheat flour 210kg, lemon acid waste sucrose solution 380kg, mix homogeneously in 60 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.9, add the Thermostable α-Amylase 290g that enzyme unit alive is 20000U/g, liquefying through secondary injection, wherein an injection temperation is 98 DEG C, and secondary injection temperature is 128 DEG C, 0.5 ~ 1h is maintained in maintaining tank, iodine examination is qualified for light brown, and obtain liquefying mixed liquid, then by 75% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:2.5, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 11%, total nitrogen 0.24%, aspergillus niger seed culture 25h, pH2.02, acidity 2.9%; Fermentation initial total sugar is 15.2%, total nitrogen 0.15%, cultivation temperature 37 DEG C; Fermentation ends acidity is 15.03% (v/v), and residual total sugar is 2.0%, and residual reducing sugar is 0.5%, and conversion ratio is 98.9%, cycle 66h. Fermentation liquid extracts citric acid through hydrogen calcium method, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 6
Semen Maydis powder 150kg, chromatograph residual liquid 600kg, mix homogeneously in 52 DEG C of material-compound tanks, adding calcium hydroxide regulates pH to 5.8, add the low pH Thermostable α-Amylase 130g that enzyme unit alive is 20000U/g, liquefying through secondary injection, wherein an injection temperation is 100 DEG C, and secondary injection temperature is 122 DEG C, maintain and tank maintains 0.5 ~ 1h, iodine examination is qualified for light brown, and obtain liquefying mixed liquid, then by 70% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:2.5, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 4.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 9.7%, total nitrogen 0.19%, aspergillus niger seed culture 26h, pH2.0, acidity 2.15%; Fermentation initial total sugar 15.90%, total nitrogen 0.10%, cultivation temperature 37 DEG C; Fermentation ends acidity is 15.42% (v/v), and residual total sugar is 2.08%, and residual reducing sugar is 0.5%, and conversion ratio is 96.14%, cycle 67h. Fermentation liquor chromatography extracts citric acid, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 batches, and citric acid fermentation level is normal.
Embodiment 7
Tapioca starch 200kg, chromatograph residual liquid 800kg, mix homogeneously in 60 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.8, add the low pH Thermostable α-Amylase 250g that enzyme unit alive is 20000U/g, liquefying through secondary injection, wherein an injection temperation is 97 DEG C, and secondary injection temperature is 124 DEG C, 0.5 ~ 1h is maintained in maintaining tank, iodine examination is qualified for light brown, and obtain liquefying mixed liquid, then by 70% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 1:1.5, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 4.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 10.9%, total nitrogen 0.3%, aspergillus niger seed culture 25h, pH2.06, acidity 2.2%; Fermentation initial total sugar is 17%, total nitrogen 0.13%, cultivation temperature 37 DEG C; Fermentation ends acidity is 16.77% (v/v), and residual total sugar is 2.15%, and residual reducing sugar is 0.5%, and conversion ratio is 98.62%, cycle 71h. Fermentation liquid extracts citric acid through chromatography, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 8
Semen Maydis powder 300kg, tapioca starch raw material 200kg, chromatograph residual liquid 1000kg, mix homogeneously in 55 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.9, adds the low pH Thermostable α-Amylase 800g that enzyme unit alive is 20000U/g, liquefies through secondary injection, wherein an injection temperation is 100 DEG C, secondary injection temperature is 130 DEG C, maintains 0.5 ~ 1h in maintaining tank, qualified for light brown through iodine examination, obtain the mixed liquid that liquefies, then by 80% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is that liquefaction clear liquid is mixed liquid mixed in 1:1.5 ratio with liquefying, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 4:1, and adds citric acid wastewater and carry out moisturizing.
Seed initial total sugar 11%, total nitrogen 0.23%, aspergillus niger seed culture 25h, pH1.99, acidity 2.3%; Fermentation medium initial total sugar is 15.44%, total nitrogen 0.11%, cultivation temperature 37 DEG C; Fermentation ends acidity is 15.38% (v/v), and residual total sugar is 2.08%, and residual reducing sugar is 0.5%, and conversion ratio is 99.64%, cycle 72h. Fermentation liquid extracts citric acid through chromatography, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
Embodiment 9
Semen Maydis powder 250kg, wheat flour 150kg, chromatograph residual liquid 1200kg, mix homogeneously in 60 DEG C of material-compound tanks, adding calcium hydroxide adjusts pH to 5.8, adds the low pH Thermostable α-Amylase 250g that enzyme unit alive is 20000U/g, liquefies through secondary injection, wherein an injection temperation is 97 DEG C, secondary injection temperature is 129 DEG C, maintains 0.5 ~ 1h in maintaining tank, and iodine examination is qualified for light brown, obtain the mixed liquid that liquefies, then by 60% the mixed liquid of liquefaction enter filter press and obtain liquefaction clear liquid.
Preparation seed culture medium and fermented nutritive liquid, wherein seed culture medium is that liquefaction clear liquid is mixed liquid mixed in 1:2 ratio with liquefying, and adds citric acid wastewater and carry out moisturizing; Fermented nutritive liquid is with liquefaction, liquefaction clear liquid to be mixed liquid mix in the ratio of 3.5:1, and adds citric acid wastewater and carry out moisturizing.
Seed culture medium initial total sugar 11.2%, total nitrogen 0.35%, aspergillus niger seed culture 24h, pH2.06, acidity 2.3%; Fermentation initial total sugar is 15.9%, total nitrogen 0.07%, cultivation temperature 37 DEG C; Fermentation ends acidity is 15.87% (v/v), and residual total sugar is 2.01%, and residual reducing sugar is 0.5%, and conversion ratio is 97.99%, cycle 63h. Fermentation liquid extracts citric acid through chromatography, produces citric acid wastewater, again according to above-mentioned steps repetitive operation, circulates 40 times, and citric acid fermentation level is normal.
The above has described embodiment of the present invention in detail; will be apparent to persons skilled in the art and can do a lot of improvement; all within the spirit and principles in the present invention, any amendment of making, equal replacement, improvement etc., should be included within the scope of protection of the invention.
Claims (2)
1. the method for citric acid wastewater backflow fermentation production of citric acid, it is characterised in that comprise the steps:
(1) starchy material and citric acid wastewater are when 50 ~ 60 DEG C, according to the ratio mix homogeneously of 1:1.5 ~ 1:4 in material-compound tank, regulating the pH to 5.8 ~ 6.0 of mixed liquor with calcium hydroxide, be subsequently adding high-temperatureα-amylase, its addition is 15 ~ 40U/g Semen Maydis powder;
(2) step (1) gained mixed liquor being carried out two-step pretreatment: first by mixed liquor through twice injection, one time injection temperation is 97 ~ 101 DEG C, and secondary injection temperature is 120 ~ 130 DEG C, be light brown and qualified through iodine examination, obtain liquefying mixed liquid;Again by mixed for the liquefaction of 60 ~ 80% liquid through filter press, obtain liquefaction clear liquid;
(3) clear liquid that liquefied by step (2) gained mixes in the ratio of 1:1.5 ~ 1:2.5 with the mixed liquid of liquefaction, and add citric acid wastewater and carry out moisturizing so that mixed liquid total sugar is 10 ~ 12%, and total nitrogen is 0.19 ~ 0.40%, obtain seed culture medium, carry out the seed culture of strain;
(4) clear liquid that liquefied by step (2) gained mixes in the ratio of 3.5:1 ~ 5:1 with the mixed liquid of liquefaction, and add citric acid wastewater and carry out moisturizing, making mixed liquid total sugar is 14 ~ 17%, total nitrogen is 0.05 ~ 0.15%, obtain fermented nutritive liquid, step (3) seed culture gained seed liquor is added fermented nutritive liquid, carries out fermentation culture;
(5) removing thalline through solid-liquid separation after step (4) fermentation ends, extract through hydrogen calcium method or chromatography and obtain citric acid, the waste water of generation returns to step (1) and step (3), (4), so circulates;
Citric acid wastewater described in step (1) refers to the useless sucrose solution that hydrogen calcium extraction method produces when manufacturing citric acid or the chromatograph residual liquid produced when chromatograph extraction method manufactures citric acid;
Strain described in step (3) is screen the strong adaptability obtained, the aspergillus niger strain that stability is high in citric acid wastewater.
2. the method for citric acid wastewater according to claim 1 backflow fermentation production of citric acid, it is characterised in that starchy material described in step (1) includes at least one of Semen Maydis powder, wheat flour, tapioca starch, sweet potato powder, starch, molasses, Semen Tritici aestivi.
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| CN105296549A (en) * | 2015-10-23 | 2016-02-03 | 安徽丰原发酵技术工程研究有限公司 | Method for improving citric acid fermenting level |
| CN105586367A (en) * | 2016-03-10 | 2016-05-18 | 江南大学 | Method for conducting fermentative production of citric acid by adding saccharifying enzyme stage by stage based on pH responses |
| CN105567749A (en) * | 2016-03-10 | 2016-05-11 | 江苏国信协联能源有限公司 | Processing method for starchiness materials and application of processing method in citric acid preparing industry |
| CN109022503B (en) * | 2018-08-17 | 2020-09-04 | 江南大学 | Method for producing citric acid by utilizing recycled anaerobic digestion solution |
| CN109022502B (en) * | 2018-08-17 | 2020-10-09 | 江南大学 | Method for producing citric acid by recycling anaerobic digestion solution |
| CN113278659A (en) * | 2021-05-28 | 2021-08-20 | 郑州运维生物技术有限公司 | Recycling method of lactic acid fermentation acid-containing wastewater |
| CN114717273A (en) * | 2022-03-10 | 2022-07-08 | 山东柠檬生化有限公司 | Recycling process of anaerobic digestion solution in citric acid production |
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| CN101942487A (en) * | 2010-10-21 | 2011-01-12 | 江南大学 | Method for fermenting and preparing citric acid by adding saccharifying enzyme |
| CN102249895A (en) * | 2011-05-10 | 2011-11-23 | 安徽丰原生物化学股份有限公司 | Treatment method of solution containing citric acid |
| CN102747111A (en) * | 2012-08-06 | 2012-10-24 | 江南大学 | Process for producing citric acid with aerobic effluent serving as ingredient water |
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| CN101942487A (en) * | 2010-10-21 | 2011-01-12 | 江南大学 | Method for fermenting and preparing citric acid by adding saccharifying enzyme |
| CN102249895A (en) * | 2011-05-10 | 2011-11-23 | 安徽丰原生物化学股份有限公司 | Treatment method of solution containing citric acid |
| CN102747111A (en) * | 2012-08-06 | 2012-10-24 | 江南大学 | Process for producing citric acid with aerobic effluent serving as ingredient water |
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