CN104109626A - Selenium-rich germinated rough rice/asparagus fermented vinegar and preparation method thereof - Google Patents
Selenium-rich germinated rough rice/asparagus fermented vinegar and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a preparation method of selenium-rich germinated rough rice/asparagus fermented vinegar, which adopts selenium-rich germinated rough rice/asparagus mixed fermentation. In the fermentation process, pectinase, amylase, saccharifying enzyme, Rhizopus oryzae CICC3099, Saccharomyces cerevisiae CICC1050 and Acetobacter pasteurianus CICC21870 are added. In the prepared vinegar, the gamma-aminobutyric acid content is 8-13 mg/100g, the vitamin content is higher than 0.3%, and the selenium content is 200 ug/kg. Therefore, the vinegar produced by the method has the advantages of multiple nutritional ingredients, aromatic flavor and soft and sweet mouthfeel, and is a vinegar product with favorable mouthfeel and nutrient and health-care effects.
Description
Technical field
The invention belongs to health care edible vinegar manufacture field, be specifically related to a kind of sprout brown rice rich in selenium asparagus fermented vinegar and preparation method thereof.
Background technology
Vinegar can generate voluntarily in physical environment, and ancient times, the mankind just started edible vinegar from very early.In China, it has been generally acknowledged that vinegar starts to be brewageed in the time of the Western Zhou Dynasty.The composition of vinegar contains the acetic acid of 3%-5% conventionally, lactic acid, and several mineral materials, what have also has a small amount of tartrate, citric acid etc.Vinegar has detumescence, detoxifies, helps digestion, effect of scattered silt, and drinking appropriate vinegar has larger help to healthy.
Asparagus formal name used at school reed bamboo shoot, its quality is fresh and tender, local flavor deliciousness, tender good to eat, in asparagus stem, contain the trace element of rich in protein, VITAMIN, mineral substance and needed by human body, in asparagus, also contain in addition the multiple steroid saponin material of distinctive asparagine, all effective in cure to cardiovascular diseases, oedema, urocystitis, leukemia, also there is anticancer effect, for a long time the edible useful taste of asparagus, human body numerous disease is had to good result for the treatment of, thereby asparagus is fabulous Dietotherapy health food.
Selenium has been used as the trace element of needed by human, has anticancer king's good reputation.It has ten large effects: 1, improve body immunity; 2, anti-oxidant, delay senility; 3, protection repair cell; 4, participate in the treatment of diabetes; 5, cancer-resisting; 6, protection eyes; 7, improve erythrocytic oxygen carrying capacity; 8, control cardiovascular and cerebrovascular diseases; 9, removing toxic substances, gas defence, antipollution; 10, protection liver.At present,
chinese Soclety of Nutritionrecommend adult's intake be 50-250 microgram every day, and China 2/3 regional selenium intake lower than minimum recommended value, therefore, Chinese generally lack selenium.Given this, rich selenium in daily edible vinegar, effectively improves the intake of selenium.
In sprout brown rice, contain γ-aminobutyric acid (GABA), it is a kind of naturally occurring nonprotein amino acid, is that inhibitory nerve important in mammalian central nervous system is passed on material, and the several functions of body is had to regulating effect.In the time that GABA in human body lacks, can produce anxiety, uneasiness, the mood such as tired, worried, need to supplement to releive mood in time.It is approved as new resource food by health ministry, has calmly neural, and anxiety, reduces blood pressure, and reduces blood ammonia, improves brain vigor, numerous effects such as release the pressure.
Now, in order to increase the health-care effect of vinegar, existing correlative study person develops health vinegar, for example: the Chinese Patent Application No. CN201010187965.8 " producing and manufacturing technique of papaya ester
", Chinese Patent Application No. CN201210162462.4 " a kind of tea vinegar beverage and preparation method thereof
"etc..What study is all to have the health vinegar of effect in a certain respect with traditional method preparation, and composition is comparatively single, and mouthfeel is general, and the vinegar based article simultaneously with desirable mouth feel and nutritional health function is less.
Summary of the invention
The present invention is directed to the deficiency of above-mentioned existence, adopt sprout brown rice rich in selenium and asparagus mixed fermentation, during the fermentation, nutritive and health protection components in asparagus and sprout brown rice rich in selenium is incorporated in vinegar, both mouthfeel and the local flavor of vinegar had been improved, strengthen again the health-care effect of sprout brown rice rich in selenium asparagus fermented vinegar, thereby the health-care effect of vinegar and quality are significantly promoted.
In order to realize object of the present invention, the inventor studies and persistent exploration by lot of experiments, has finally obtained following technical scheme:
A kind of sprout brown rice rich in selenium asparagus fermented vinegar, is characterized in that: described sprout brown rice rich in selenium asparagus fermented vinegar is mainly prepared from by following weight by following raw material: asparagus 40-50kg, water 300-350kg, brown rice 80-120kg.
The preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar of the present invention, comprises the following steps:
(1) preparation of asparagus enzymolysis solution: get asparagus 40-50kg fresh, that cleaned, after being cut into segment, use colloidal mill defibrination, in slurries, add water 300-350kg, after mixing, press the polygalacturonase 0.70-0.90g/kg that adds enzyme 6000U/g alive with the weight ratio (g/kg) of raw material, enzymolysis 14-18h under 45-50 DEG C of condition, obtains enzymolysis solution;
(2) saccharification of sprout brown rice rich in selenium slurries: by 80-120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtaining with step (1) mixes, press the amylase 0.60-0.90g/kg that adds enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.40-0.70g/kg of enzyme 10000 U/g alive, saccharification 9-12h under 31-36 DEG C of condition;
(3) distiller's wort preparation: in the saccharified liquid obtaining in step (2), by adding Rhizopus oryzae 0.45-0.55g/kg with the weight ratio (g/kg) of raw material, yeast saccharomyces cerevisiae 0.75-0.90g/kg, is placed in 35-40 DEG C of condition bottom fermentation and obtains distiller's wort for 10-13 days;
(4) distiller's wort solid state fermentation: in the distiller's wort obtaining in step (3), by adding Acetobacter pasteurianus CICC21870 0.70-0.90g/kg with the weight ratio (g/kg) of raw material, adopt solid state fermentation 7-10 days under 37-42 DEG C of condition, obtain fermented liquid;
(5) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the sprout brown rice rich in selenium preparation method described in step (2) is: wrap brown rice with gauze, in 2-8 mole/L growth hormone solution, soak 15-25h, then be placed in 25-35 DEG C of constant incubator and cultivate germination, and every once rich selenium flushing liquor of 4h spray, in the time that bud grows to 1-2cm length, take out clean draining, obtain sprout brown rice rich in selenium.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, the preparation method who it is characterized in that rich selenium flushing liquor is: the rich selenium coal gangue pulverizing article of cut-off footpath between 60-100 order, be placed in 20-30%(V/V) sulfurous acid solution soak 20-50h, use ultrasonic concussion simultaneously, accelerate the dissolving of selenium element; After immersion, add 5-10%(V/V) sodium radio-phosphate,P-32 solution, the heavy metal in selenium ore is precipitated, filtered, in filtered liquid, adding concentration is 8-13%(V/V) sodium hydroxide solution, the pH regulator of filtered liquid, to 6.5-8.5, is made to flushing liquor.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the Rhizopus oryzae described in step (3) is that Rhizopus oryzae CICC3099, yeast saccharomyces cerevisiae are yeast saccharomyces cerevisiae CICC1050.
Preferably, the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar as above, wherein the Acetobacter pasteurianus described in step (4) is Acetobacter pasteurianus CICC21870.
The present invention is compared with existing vinegar processing technology, and tool has the following advantages:
(1) adopt solid fermentating mode, utilize the enzyme that enriches that sprout brown rice rich in selenium produces to be, raw material glycolysis vinegar nutritive ingredient more abundant, that make is increased, local flavor sweet-smelling, mouthfeel is soft sweet refreshing, and quality is improved.
(2) utilize green plants asparagus and sprout brown rice rich in selenium to mix and produce vinegar, by the VITAMIN of enriching of asparagus enzymolysis generation, amino acid, the nutritive and health protection components such as trace element and sprout brown rice rich in selenium is dissolved in vinegar, in the vinegar that finally can make to make, alpha-aminobutyric acid content reaches 8-12mg/100g, and vitamin contents reaches 0.3%, selenium content 200ug/Kg, thereby the vinegar that makes to make reaches that both nutrient health was safe and reliable, there is again good health-care effect.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done to further description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
embodiment 1
(1) preparation of asparagus enzymolysis solution: get asparagus 40kg fresh, that cleaned, after being cut into segment, use colloidal mill defibrination, in slurries, add water 300kg, after mixing, press the polygalacturonase 0.70g/kg that adds enzyme 6000U/g alive with the weight ratio (g/kg) of raw material, enzymolysis 14h under 45 DEG C of conditions, obtains enzymolysis solution;
(2) preparation that liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing articles of cut-off footpath 60 objects, are placed in 20%(V/V) sulfurous acid solution soak 20h, use ultrasonic concussion simultaneously, accelerate the dissolving of selenium element; After immersion, add 5%(V/V) sodium radio-phosphate,P-32 solution, the heavy metal in selenium ore is precipitated, filtered, in filtered liquid, adding concentration is 8%(V/V) sodium hydroxide solution, by the pH regulator to 6.5 of filtered liquid, make flushing liquor;
(3) preparation of sprout brown rice rich in selenium:: wrap brown rice with gauze, in 2 moles/L growth hormone solution, soak 15h, then be placed in 25 DEG C of constant incubators and cultivate germination, and every once rich selenium flushing liquor of 4h spray, in the time that bud grows to 1cm length, take out clean draining, obtain sprout brown rice rich in selenium;
(4) saccharification of sprout brown rice rich in selenium slurries: by 80kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtaining with step (1) mixes, press the amylase 0.60g/kg that adds enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.40g/kg of enzyme 10000 U/g alive, saccharification 9h under 31 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid obtaining in step (2), by adding Rhizopus oryzae 0.45g/kg with the weight ratio (g/kg) of raw material, yeast saccharomyces cerevisiae 0.75g/kg, is placed in 35 DEG C of condition bottom fermentations and obtains distiller's wort for 10 days;
(6) distiller's wort solid state fermentation: in the distiller's wort obtaining in step (3), by adding Acetobacter pasteurianus 0.70g/kg with the weight ratio (g/kg) of raw material, adopt solid state fermentation 7 days under 37 DEG C of conditions, obtain fermented liquid;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
embodiment 2
(1) preparation of asparagus enzymolysis solution: get asparagus 50kg fresh, that cleaned, after being cut into segment, use colloidal mill defibrination, in slurries, add water 350kg, after mixing, press the polygalacturonase 0.90g/kg that adds enzyme 6000U/g alive with the weight ratio (g/kg) of raw material, enzymolysis 18h under 50 DEG C of conditions, obtains enzymolysis solution;
(2) preparation that liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing articles of cut-off footpath 100 objects, are placed in 30%(V/V) sulfurous acid solution soak 50h, use ultrasonic concussion simultaneously, accelerate the dissolving of selenium element; After immersion, add 10%(V/V) sodium radio-phosphate,P-32 solution, the heavy metal in selenium ore is precipitated, filtered, in filtered liquid, adding concentration is 13%(V/V) sodium hydroxide solution, by the pH regulator to 8.5 of filtered liquid, make flushing liquor;
(3) preparation of sprout brown rice rich in selenium: wrap brown rice with gauze, in 8 moles/L growth hormone solution, soak 25h, then be placed in 35 DEG C of constant incubators and cultivate germination, and every once rich selenium flushing liquor of 4h spray, in the time that bud grows to 2cm length, take out clean draining, obtain sprout brown rice rich in selenium;
(4) saccharification of sprout brown rice rich in selenium slurries: by 120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtaining with step (1) mixes, press the amylase 0.90g/kg that adds enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.70g/kg of enzyme 10000 U/g alive, saccharification 12h under 36 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid obtaining in step (2), by adding Rhizopus oryzae 0.55g/kg with the weight ratio (g/kg) of raw material, yeast saccharomyces cerevisiae 0.90g/kg, is placed in 40 DEG C of condition bottom fermentations and obtains distiller's wort for 13 days;
(6) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus 0.90g/kg with the weight ratio (g/kg) of raw material, adopt solid state fermentation 10 days under 42 DEG C of conditions, obtain fermented liquid;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
embodiment 3
(1) preparation of asparagus enzymolysis solution: get asparagus 45kg fresh, that cleaned, after being cut into segment, use colloidal mill defibrination, in slurries, add water 325kg, after mixing, press the polygalacturonase 0.80g/kg that adds enzyme 6000U/g alive with the weight ratio (g/kg) of raw material, enzymolysis 16h under 47 DEG C of conditions, obtains enzymolysis solution;
(2) preparation that liquid is shone in the spray of rich selenium: the rich selenium coal gangue pulverizing articles of cut-off footpath 80 objects, are placed in 25%(V/V) sulfurous acid solution soak 35h, use ultrasonic concussion simultaneously, accelerate the dissolving of selenium element; After immersion, add 7%(V/V) sodium radio-phosphate,P-32 solution, the heavy metal in selenium ore is precipitated, filtered, in filtered liquid, adding concentration is 10%(V/V) sodium hydroxide solution, by the pH regulator to 7.5 of filtered liquid, make flushing liquor.
(3) preparation of sprout brown rice rich in selenium: wrap brown rice with gauze, in 5 moles/L growth hormone solution, soak 20h, then be placed in 30 DEG C of constant incubators and cultivate germination, and every once rich selenium flushing liquor of 4h spray, in the time that bud grows to 1.5cm length, take out clean draining, obtain sprout brown rice rich in selenium.
(4) saccharification of sprout brown rice rich in selenium slurries: by 100kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtaining with step (1) mixes, press the amylase 0.75g/kg that adds enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.50g/kg of enzyme 10000 U/g alive, saccharification 11h under 34 DEG C of conditions;
(5) distiller's wort preparation: in the saccharified liquid obtaining in step (2), by adding Rhizopus oryzae 0.50g/kg with the weight ratio (g/kg) of raw material, yeast saccharomyces cerevisiae 0.85g/kg, is placed in 38 DEG C of condition bottom fermentations and obtains distiller's wort for 12 days;
(6) distiller's wort solid state fermentation: in the distiller's wort that step (3) obtains, by adding Acetobacter pasteurianus 0.80g/kg with the weight ratio (g/kg) of raw material, adopt solid state fermentation 9 days under 40 DEG C of conditions, obtain fermented liquid;
(7) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
comparative example 1:
Supplementary material kind, the fermentation condition in interpolation quantity, order of addition and each stage that this embodiment uses with embodiment 1 are all identical, only in comparative example, brown rice are not carried out to selenium-rich germinated processing.
comparative example 2:
In this embodiment, do not use the enzymolysis solution of asparagus, lack the step (1) in example 2, all the other steps are all identical with example 2.
comparative example 3:
the difference of this embodiment and example 3 is not add zymin in step (1) in comparative example 3, (4), and other are all identical with example 3.
Asparagus vinegar prepared by embodiment 1-3 and comparative example 1-3, its attributional analysis is as following table:
The each embodiment attributional analysis of table 1 table
From above data, the asparagus vinegar that uses the present invention to prepare, VITAMIN, protein, amino acid and enzyme equal size are all higher than comparison example, especially in comparative example 1, brown rice is not carried out to selenium-rich germinated processing, r-Gamma-propalanine content is 0, selenium content is 0, does not use asparagus raw material in comparison example 2, and in the vinegar of preparation, vitamin contents is extremely low.Therefore, what use that the sprout brown rice rich in selenium asparagus fermented vinegar prepared of the present invention makes full use of that the glycolysis such as sprout brown rice rich in selenium and asparagus produces enriches enzyme system, VITAMIN and macroelement, trace element, the vinegar nutritive ingredient making is many, local flavor sweet-smelling, mouthfeel is soft sweet refreshing, is the vinegar based article that simultaneously possesses splendid mouthfeel and nutritional health function.
Claims (6)
1. a sprout brown rice rich in selenium asparagus fermented vinegar, is characterized in that: described sprout brown rice rich in selenium asparagus fermented vinegar is mainly prepared from by following weight by following raw material: asparagus 40-50kg, water 300-350kg, brown rice 80-120kg.
2. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 1, is characterized in that the method comprises the following steps:
(1) preparation of asparagus enzymolysis solution: get asparagus 40-50kg fresh, that cleaned, after being cut into segment, use colloidal mill defibrination, in slurries, add water 300-350kg, after mixing, press the polygalacturonase 0.70-0.90g/kg that adds enzyme 6000U/g alive with the weight ratio (g/kg) of raw material, enzymolysis 14-18h under 45-50 DEG C of condition, obtains enzymolysis solution;
(2) saccharification of sprout brown rice rich in selenium slurries: by 80-120kg sprout brown rice rich in selenium colloidal mill defibrination, then the enzymolysis solution obtaining with step (1) mixes, press the amylase 0.60-0.90g/kg that adds enzyme 9000U/g alive with the weight ratio (g/kg) of raw material, the saccharifying enzyme 0.40-0.70g/kg of enzyme 10000 U/g alive, saccharification 9-12h under 31-36 DEG C of condition;
(3) distiller's wort preparation: in the saccharified liquid obtaining in step (2), by adding Rhizopus oryzae 0.45-0.55g/kg with the weight ratio (g/kg) of raw material, yeast saccharomyces cerevisiae 0.75-0.90g/kg, is placed in 35-40 DEG C of condition bottom fermentation and obtains distiller's wort for 10-13 days;
(4) distiller's wort solid state fermentation: in the distiller's wort obtaining in step (3), by adding Acetobacter pasteurianus 0.70-0.90g/kg with the weight ratio (g/kg) of raw material, adopt solid state fermentation 7-10 days under 37-42 DEG C of condition, obtain fermented liquid;
(5) sprout brown rice rich in selenium asparagus fermented vinegar preparation: fermented liquid is centrifugal, filters, sterilizing, and packaging, can obtain sprout brown rice rich in selenium asparagus fermented vinegar.
3. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 2, it is characterized in that: described in step (2), the preparation method of sprout brown rice rich in selenium is: wrap brown rice with gauze, in 2-8 mole/L growth hormone solution, soak 15-25h, then be placed in 25-35 DEG C of constant incubator and cultivate germination, and every once rich selenium flushing liquor of 4h spray, in the time that bud grows to 1-2cm length, take out clean draining, obtain sprout brown rice rich in selenium.
4. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 3, the preparation method who it is characterized in that rich selenium flushing liquor is: the rich selenium coal gangue pulverizing article of cut-off footpath between 60-100 order, be placed in 20-30%(V/V) sulfurous acid solution soak 20-50h, use ultrasonic concussion simultaneously, accelerate the dissolving of selenium element; After immersion, add 5-10%(V/V) sodium radio-phosphate,P-32 solution, the heavy metal in selenium ore is precipitated, filtered, in filtered liquid, adding concentration is 8-13%(V/V) sodium hydroxide solution, the pH regulator of filtered liquid, to 6.5-8.5, is made to flushing liquor.
5. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 2, is characterized in that: the described Rhizopus oryzae of step (3) is that Rhizopus oryzae CICC3099, yeast saccharomyces cerevisiae are yeast saccharomyces cerevisiae CICC1050.
6. the preparation method of a kind of sprout brown rice rich in selenium asparagus fermented vinegar according to claim 2, is characterized in that: the described Acetobacter pasteurianus of step (4) is Acetobacter pasteurianus CICC21870.
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| CN104109626B (en) | 2016-02-10 |
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