CN104106459B - The unsettled water planting seedling-cultivating method of lily bud scale - Google Patents
The unsettled water planting seedling-cultivating method of lily bud scale Download PDFInfo
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- CN104106459B CN104106459B CN201410358151.4A CN201410358151A CN104106459B CN 104106459 B CN104106459 B CN 104106459B CN 201410358151 A CN201410358151 A CN 201410358151A CN 104106459 B CN104106459 B CN 104106459B
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- cuttage
- lily bud
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Abstract
The present invention discloses a kind of unsettled water planting seedling-cultivating method of lily bud scale, the method is sponge cutting medium being placed in unsettled water planting, lily bud scale cuttage is in active carbon culture matrix, 12 h light by day, intensity of illumination 5500Lx, evening 12 h dark, temperature 25 DEG C condition under cultivate, cultivation cycle is 60-80 days, within every 7 days, change first water, morning every day, evening spray nutrient solution to cuttage scale.The unsettled water planting seedling-cultivating method of lily bud scale of the present invention, operate simple and easy, material source is wide, cost of investment is low, there is provided better matrix environment can to cuttage scale, significantly improve rooting rate, sprout rate, growth coefficient, coefficient etc. of taking root compared with training with traditional sand, in cuttage process, the rotting rate of scale and lethality are close to zero.The inventive method is simple and easy, convenient, popularization is easy, improves the survival rate of lily bud scale cuttage, reduces seedling cost.
Description
Technical field
The present invention relates to plant asexual reproduction method, particularly a kind of unsettled water planting seedling-cultivating method of lily bud scale.
Background technology
Vegetative propagation not only can keep its excellent moral character, can be large-scale industrialized nursery simultaneously and lays the foundation.Current tissue culture propagation method, technology and condition of culture require high, not easy to operate, should not promote, and cuttage are simple to operate, and condition of culture requires lower, suitable popularization.But the survival rate of cuttage branch is not high in actual production, survival rate, except by except the nutriment of institute's cuttage species inner accumulation and the restriction of endogenous hormones, is also subject to the impact of the extraneous factors such as temperature, humidity, illumination, gas permeability.Cuttage branch respiration in rooting process is vigorous especially, and whether oxygen one of abundance essential condition that can be cuttings survive.For this problem, current more scholar thinks that good matrix is organic and the mixed-matrix of inorganic matrix composition.But this type of matrix cost is higher and not easily obtain, be not suitable for promoting.
Tradition Water culture cultural method, the factor of most critical is the dissolved oxygen amount in water, and content of oxygen dissolved in water is lower, is unfavorable for the growth of branch root root system.Generally adopt bubble method, a mist method, flow liquid method to increase dissolved oxygen in water or add oxygenation agent.The hot and humid rooting of cuttings that is conducive to also is beneficial to bacterial reproduction, and cutting in water planting is once by bacteriological infection, and consequence comparatively earth culture is more serious.In order to reduce bacterium to the infringement of cutting, improve cutting survival rate, reply cutting carries out disinfection sterilization processing.Visible traditional water planting will ensure dissolved oxygen amount, and it is higher to reduce cost needed for infection, and complicated operation.The sand water-holding capacity of the husky training of tradition is lower, and is unfavorable for root system development.Because the specific surface area relative activity charcoal of sand is much smaller, adsorption capacity, also much smaller than active carbon, causes plant root cleannes lower, is easily encroached on by miniature organism, the more difficult control of moisture supply of husky training.
Current lily breeding is domestic key subjects, and scale cuttage is main a kind of breeding practice, and the research for lily bud scale cuttage is also more.The most frequently used is the method breaking lily bulb dormancy by low temperature treatment at present, and the method can improve blade pumping rate, and pumping rate can reach 51%-61%, but lower, and complicated operation; Separately have and cultivate after garden mould and river sand by a certain percentage proportioning, but survival rate is not high.
Summary of the invention
The technical problem to be solved in the present invention is the deficiency for overcoming existing lily cut flowers cuttage, and provides a kind of survival rate high, and low cost is simple to operate, the unsettled water planting seedling-cultivating method of the lily bud scale that popularization degree is high.
Realizing technical scheme of the present invention is:
A unsettled water planting seedling-cultivating method for lily bud scale, comprises the steps:
(1) seedbed prepares: get one piece of rectangular sponge, sponge distilled water is cleaned, and with 70% alcohol-pickled washing 20-30min, after washing away alcohol with distilled water again, the doubling of long limit surrounds a drum, is tied up at 2/3 place of drum sponge, does not stay gap to be advisable with the sponge at the place of tying up, insert active carbon in the bottom of cavernous body top hollow region, bottom is placed in the container that distilled water is housed;
(2) choose without damage by disease and insect, healthy lily bulb, the skin getting bulb be loose, epidermis without the scale of disability as cuttage material;
(3) lily bud scale of step (2) is cleaned, put into after bactericide soaks 25-30 minute, then use GA
31000mg/L soaks 5-5.5 hour, pulls out, cleans, dries;
(4) the base portion cuttage of the lily bud scale of step (3) is cultivated in the active carbon of the cavernous body of step (1); Condition of culture is daylight 12 hours, intensity of illumination 5500Lx; Evening dark processing 12 hours, temperature 25 DEG C; Cultivation cycle is 60-80 days, within every 7 days, changes first water, and morning every day, evening spray nutrient solution to cuttage body respectively and drenched on scale surface.
The packed height of described active carbon is 1 ~ 2cm.
Described bactericide is 50% carbendazol wettable powder, 1000 times of liquid.
Described nutrient solution is Hoagland ' s(Gram suddenly) nutrient solution, and pH is 6.0-6.2.
Easily there is the situation that scale rots in conventional lily bud scale cuttage, the unsettled water planting seedling-cultivating method of lily bud scale of the present invention has new breakthrough in the enhancement of environment of scale root growth, can solve ventilative difficult problem by the unsettled setting of root by sponge; Sponge and active carbon all have water imbibition, this key factor of water binding capacity can be solved, sponge in water body and the surface of active carbon can form huge biomembrane, strengthen the detergent power to water body, make the water in bottle comparatively clean, avoid plant root to be injured by germ, obviously alleviate rotting of scale, improve the survival rate of nursery.
The advantage of the unsettled water planting seedling-cultivating method of lily bud scale of the present invention is: survival rate is high, low cost, simple to operate.
Accompanying drawing explanation
Fig. 1, Fig. 2 are the plant that the unsettled water planting growing nursery and culture of the embodiment of the present invention 1 Lilium lancifo1ium Thunb goes out;
Fig. 3, Fig. 4 are the husky plant cultivated seedling and cultivate of Lilium lancifo1ium Thunb tradition;
Fig. 5 is the plant that the unsettled water planting growing nursery and culture of the embodiment of the present invention 2 Fragrant Lily goes out;
Fig. 6 is the husky plant cultivated seedling and cultivate of Fragrant Lily tradition.
Embodiment
Below in conjunction with embodiment, content of the present invention is further described, but is not limitation of the invention.
Embodiment 1
A unsettled water planting seedling-cultivating method for lily bud scale, for Lilium lancifo1ium Thunb, comprises the steps:
The collection of Lilium lancifo1ium Thunb scale: gather a collection of without damage by disease and insect, healthy Lilium lancifo1ium Thunb plant on flowers market, strips the lily bud scale of outer loose, epidermis without disability.
Matrix configures: get one piece of rectangular sponge, sponge distilled water is cleaned, and with 70% alcohol-pickled washing 20min, after washing away alcohol with distilled water again, the doubling of long limit surrounds a drum, is tied up at 2/3 place of drum sponge, does not stay gap to be advisable with the sponge at the place of tying up, insert the powdered activated carbon of 1 ~ 2cm height in the bottom of cavernous body top hollow region, bottom is placed in the vial that distilled water is housed.
Cuttage process and cuttage: cleaned by the Lilium lancifo1ium Thunb scale clear water chosen, put into 50% carbendazim and dilute 1000 times of immersions 30 minutes, then use GA
31000mg/L soaks 5 hours, cleaner with distilled water flushing after, dry scale surface moisture.Place completely in the active carbon being contacted with unsettled cavernous body with by Lilium lancifo1ium Thunb scale base portion.Using unsettled cavernous body to carry powdered activated carbon makes the loose structure of sponge and active carbon easily form huge biomembrane, can strengthen water body purification ability, and the good physico chemical adsorption ability of active carbon, make the moisture of plant basal sink comparatively clean.
Daily management after cuttage: the mainly daylight 12 hours of the management after cuttage, intensity of illumination 5500Lx; Evening dark culturing 12 hours, temperature controls at about 25 DEG C, within every 7 days, changes first water, and every day is early, evening sprays Hoagland ' s(Gram suddenly to cuttage plant) nutrient solution.
Adopt the unsettled water planting of the present invention to train contrast ratio pair with husky, experimental result is in table 1:
Table 1: Lilium lancifo1ium Thunb scale culture 80 days experimental results
With reference to Fig. 1-4, the experiment proved that and adopt the unsettled water planting seedling-cultivating method of the present invention, Lilium lancifo1ium Thunb blade pumping rate reaches 76.67%, and average height of sprouting is 6.10cm, and the color of leaf is dominant with green; The rooting rate of scale is about 83.33%, and coefficient of taking root is 2.03, and value-added coefficient is 1.73, and every result is all cultivated higher than sandy soil.
Embodiment 2
A unsettled water planting nursery for lily bud scale, for Fragrant Lily, method step is with embodiment 1.
Adopt the unsettled water planting of the present invention to train contrast ratio pair with husky, experimental result is in table 2:
Table 2: Fragrant Lily scale culture 60 days experimental results
With reference to Fig. 5-6, the experiment proved that and adopt the unsettled water planting seedling-cultivating method of the present invention, Fragrant Lily scale rooting rate reaches 93.33%, and coefficient of taking root is 2.4, and value-added coefficient is 3.23, and every result is all cultivated higher than sandy soil.
Claims (4)
1. the unsettled water planting seedling-cultivating method of lily bud scale, is characterized in that: comprise the steps:
(1) seedbed prepares: get one piece of rectangular sponge, sponge distilled water is cleaned, and with 70% alcohol-pickled washing 20-30min, after washing away alcohol with distilled water again, the doubling of long limit surrounds a drum, and tied up at 2/3 place of drum sponge, the sponge at the place of tying up does not stay gap, insert active carbon in the bottom of cavernous body top hollow region, bottom is placed in the container that distilled water is housed;
(2) choose without damage by disease and insect, healthy lily bulb, the skin getting bulb be loose, epidermis without the lily bud scale of disability as cuttage material;
(3) lily bud scale of step (2) is cleaned, put into after bactericide soaks 25-30 minute, then use GA
31000mg/L soaks 5-5.5 hour, pulls out, cleans, dries;
(4) the base portion cuttage of the lily bud scale of step (3) is cultivated in the active carbon of the cavernous body of step (1); Condition of culture is daylight 12 hours, intensity of illumination 5500Lx; Evening dark processing 12 hours, temperature 25 DEG C; Cultivation cycle is 60-80 days, within every 7 days, changes first water, and morning every day, evening spray nutrient solution to cuttage body respectively and drenched on lily bud scale surface.
2. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: the packed height of step (1) described active carbon is 1 ~ 2cm.
3. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: step (3) described bactericide is 50% carbendazol wettable powder, 1000 times of liquid.
4. the unsettled water planting seedling-cultivating method of lily bud scale according to claim 1, is characterized in that: the nutrient solution of Gram suddenly of step (4) described nutrient solution to be pH be 6.0-6.2.
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| CN106857206A (en) * | 2017-01-12 | 2017-06-20 | 浙江省林业科学研究院 | A kind of D. primulinum Hydroponic culture and cultural method |
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| CN1460409A (en) * | 2003-06-04 | 2003-12-10 | 中国科学院昆明植物研究所 | Quick breeding method of lanzhou lily |
| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN102972198A (en) * | 2012-12-25 | 2013-03-20 | 连云港市农业科学院 | Method for enlarging oriental lily scale cutting ball |
| CN103371045A (en) * | 2012-04-28 | 2013-10-30 | 阎立燕 | Reproduction method for lilium davidii unicoior cotton |
| CN203467354U (en) * | 2013-08-23 | 2014-03-12 | 中国热带农业科学院环境与植物保护研究所 | Water-planting device for tissue culture seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20000056509A (en) * | 1999-02-23 | 2000-09-15 | 강영규 | Nutrient composition of the lily and method for culturing the lily using container |
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| CN1460409A (en) * | 2003-06-04 | 2003-12-10 | 中国科学院昆明植物研究所 | Quick breeding method of lanzhou lily |
| CN101711493A (en) * | 2009-11-11 | 2010-05-26 | 玉溪明珠花卉股份有限公司 | Propagation of lily seedball by directly peeling scale |
| CN103371045A (en) * | 2012-04-28 | 2013-10-30 | 阎立燕 | Reproduction method for lilium davidii unicoior cotton |
| CN102972198A (en) * | 2012-12-25 | 2013-03-20 | 连云港市农业科学院 | Method for enlarging oriental lily scale cutting ball |
| CN203467354U (en) * | 2013-08-23 | 2014-03-12 | 中国热带农业科学院环境与植物保护研究所 | Water-planting device for tissue culture seedlings |
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| GA3、IBA以及不同基质对精粹百合鳞片扦插繁殖的影响;孙红梅等;《林业科学》;20081215;第42卷(第12期);第63~69页 * |
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| 苎麻嫩梢悬空喷雾育苗研究初报;刘素纹;《湖北农业科学》;19890730(第7期);第14~15页 * |
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