CN104099250A - Chaetoceros curvisetus culture medium with cow dung extract as main raw material and use thereof - Google Patents
Chaetoceros curvisetus culture medium with cow dung extract as main raw material and use thereof Download PDFInfo
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Abstract
The invention belongs to the aquaculture field and in particular relates to a use method of dairy farm excrement in strongylocentrotus nudus seedling culture. Chaetoceros curvisetus is traditionally cultured according to the formula of Zhanjiang Fisheries College and in an open water mud pool, and is slow in growth, prone to pollute, high in cost, low in benefit and high in workload; now, the chaetoceros curvisetus is cultured in a storage reservoir, and such nutritional components as cow dung extract, ammonium nitrate, soil extract, sea mud extract, trypsinogen and hydroxyacetic acid are added to the culture medium, and therefore, the dairy farm excrement can be treated at a low cost, the waste is utilized and changed into things of value and the chaetoceros curvisetus having important bait value can be obtained, and moreover, double effects of protecting the environment and producing the biological resources are achieved; organic and inorganic fertilizers are used together to provide more comprehensive and balanced nutrition; the use method is convenient to operate, low in cost and high in benefit; the growth rate and the yield of the chaetoceros curvisetus are greatly increased, and the yield is increased by 320%.
Description
Technical field
The invention belongs to aquaculture field, relate to take that cow dung leach liquor is main raw material revolve chain angle hair algae culture medium and application thereof.
Background technology
Along with greatly developing of milk cattle cultivating industry, cow dung processing has become the problem of government and milk cow owner headache.The fresh excrement that every adult dairy cattle is drained every day is 40-50 kilogram, and the fresh excrement that standby milk cow drains every day is 10-20 kilogram, and it is 30-40 ton that fresh cow dung is discharged every day in thousand cow head fields, within 1 year, discharges fresh cow dung up to 10000 tons.Many countries of beginning the sixties in 20th century come to realise the pollution problem of livestock and poultry fecaluria, and take measures to be intervened and limit, and Japan has formulated many relevant fecal pollution things and processed rules; It is illegal act that Germany, Holland's regulation are directly discharged to fecaluria in surface water; The U.S. require to build livestock and poultry farm must and focus on the exploitation of waste resource by environmental permission.Diary farm cow dung and filth disposal are the thorny problem of pendulum in face of us.
One, the present situation of milk cow cow dung and subject matter
1, raising farm cow dung is stacked problem
2000 of cattle farms, Beijing ox scale, takes up an area 500 mu, and cow dung accumulation is taken up an area 150 mu of left and right.1000 of cattle farms, Chongqing scale, cattle farm ight soil tip 5-10 mu left and right.As long as there is week age cow dung not to be pulled away, will pile up like a mountain in, mix with sewage, milk cow is all day long in muddy middle existence.
2, cow dung and water pollute
Due to cow dung Chang Yuniu urine, milk Room sewage, rinse ground sewage, life and mix with sewage etc., formation excrement is dirty, flow is very large, the dirty processing of excrement is not done in general milk cow place, most of cattle farm, the ground such as Beijing, Shanxi, Hebei, Heilungkiang, the Inner Mongol does not have fecal sewage treatment mechanical means, some excrement dirt is discharged near gully, somely excrement dirt is discharged into the dirty treating pond of excrement is temporarily deposited or buried, some excrement dirt is discharged into farmland, polluted around water source and environment, caused that local government and peasant are discontented.
3, the other problems of cow dung
The vehicle wheel vehicle body of often seeing diary farm cleaning ight soil is stained with ight soil everywhere, and plant road surface also cow dung everywhere, is cow dung on workman's clothes entirely.Office, dormitory even dining room have cow dung.Cow dung is piled up, and filthy crossing current, causes mosquitos and flies, bacterium and other microorganisms, brings very big inconvenience to diary farm and surrounding area.The cow dung of piling up is in addition polluted air, soil and water source also.
Two, the tradition of cow dung is utilized method and shortcoming both at home and abroad
It is following several that the tradition of cow dung utilizes method to have, in Table 1.
Table 1 both at home and abroad cow dung tradition utilizes method
But above method more or less exists the problems such as technology is immature, economic benefit is low, troublesome poeration, technical process is long, initial cost is large, pile up like a mountain, with serious pollution problem can not fundamentally to solve cow dung.
In order to solve cow dung tradition, utilize the shortcoming of method, the invention provides and a kind ofly take diary farm ight soil and cultivate the most important open-mouthed bait of the Strongylocentrotus nudus young-the revolve method of chain Chaetoceros as main raw material, can turn out the marine algae of food value, can open up new road for dealing carefully with diary farm ight soil again.
Revolving chain Chaetoceros Chaetoceros curvisetus is a kind of marine microalgae, is subordinate to Bacillariophyta, center guiding principle, box-like Cutleriales, Chaetoceros section, Chaetoceros genus.Shell face ellipse or subcircular, have two angle hairs.Girdle face quadrangle, has two obvious bands.The angle hair of adjacent cells is connected, and makes colony become chain.The length of angle hair, often times over cell paste itself.Spirrillum colony, wide girdle face size 7-30 μ m.Angle capillary and level and smooth, bears from cell angle, all bends towards a side of chain projection, and the form of end of the chain angle hair is normal different with other angle hairs, short and thick.After the capable repeatedly indirect division of cell, in chain, there is different angle hair, so be divided into two chains.Chromatoplast two or more, also has chromatoplast in the hair of some kinds angle, is one of minute kind of feature.Reproduction method is to form auxospore, hypnospore or sexual propagation.In fossil, occurring, is often its statospore.For the littoral kind of eurythermic, warm season distributes more, and all there is distribution in China's the East China Sea and the Yellow Sea and the Bohai Sea.Revolving chain Chaetoceros and in marine plankton, account for critical positions, is fish, shrimp, crab, sea cucumber, the good foodstuff of sea urchin.Particularly in sea urchin artificial breeding, revolve chain Chaetoceros and be the Strongylocentrotus nudus young most important, happiness food open-mouthed bait, can provide to be in time of high nutritive value and the chain Chaetoceros that revolves of abundant amount just has decisive role to grow seedlings success or failure and output of Strongylocentrotus nudus.
Revolving the cultivation of chain Chaetoceros is to adopt Zhanjiang Aquatic Production College's formula (ammonium nitrate 5-20 milligram traditionally, potassium primary phosphate 0.5-1 milligram, ironic citrate 0.5-2 milligram, 1000 milliliters, seawater of sterilization) and adopt open cement pit to cultivate, growth is slow, easily pollute, cost is high, benefit is low, operation effort, workload is large, I adopt retention basin cultivation and in substratum, have added cow dung leach liquor now, ammonium nitrate, people's urine, urea, potassium silicate, potassium primary phosphate, fish meal, triacontanol price quote, chondroitin sulfate, marine yeast, bread fresh yeast, tyrosine, Vltra tears, L-arginine, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, soil extract, ooze leach liquor, Trypsin, Tutofusin tris, the nutritive ingredients such as oxyacetic acid, not only can low-cost processes diary farm ight soil, utilization of waste material, turn waste into wealth, and can obtain important food value revolve chain Chaetoceros, achieve many things at one stroke, really be the ingenious utmost point, simply fantastic.Fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, balanced, easy to operate, and cost is low, and high efficiency is revolved the chain Chaetoceros speed of growth and output and significantly improved, and culture cycle shortens, and output improves 320%.
Summary of the invention
In order to overcome the deficiency of revolving at present chain Chaetoceros culture technique, the invention provides a kind of take that cow dung leach liquor is main raw material revolve chain angle hair algae culture medium, fill a prescription as follows:
150 milliliters of cow dung leach liquors, 4 milliliters, ammonium nitrate, people urinates 1 milliliter, 2 milligrams, urea, 0.5 milligram of potassium silicate, 5 milligrams of potassium primary phosphates, 4 milligrams of fish meal, 0.2 milligram of triacontanol price quote, 2 milligrams of chondroitin sulfates, 0.5 milligram of marine yeast, 1 milligram of bread fresh yeast, 0.8 milligram, tyrosine, 0.6 milligram of Vltra tears, 1 milligram of L-arginine, 6 milligrams of medical stone powders, 920 plant growth substance 4 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 1.2 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 6 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 1 milliliter of oxyacetic acid, 1000 milliliters, the pure seawater of ozonization.
Utilization utilizes diary farm ight soil leach liquor to revolve a chain angle hair algae method for main raw material cultivation, and concrete steps are as follows:
Culture vessel is volume 3m
3water butt, market is on sale, and food grade high-density polyethylene material is made, thickening, seamless integral structure, sturdy and durable, shock resistance, water white transparency, has good light permeability, in order to revolving chain Chaetoceros, carries out photosynthesis.Have a water tap near bottom, a circular lid is arranged at bucket top, above has the silk of revolving, rotatable and seal, and lid is stamped 3 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, 6 millimeters of internal diameters.Also have a hole to penetrate a plain conductor, wire end connects a 40w electricity-saving lamp.Because bucket is higher, there is a ladder on bucket side, convenient operation up and down.Water butt is washed, with distilled water rinse 3 times, pack 2 tons of substratum claimed in claim 1 into afterwards, inflation mixes 5 minutes, selects vigorous, the free of contamination inoculation of chain Chaetoceros, the inoculum density 4 * 10 of revolving of vitality
4cell/mL; On lid, insert two clean Glass tubings, one longer, 100 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone is to form very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 35 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, is filled with mixed gas, and mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, with frustule, can not float; Outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-8000Lux; Opening electricity-saving lamp night throws light on.Cultivate first 5 days, a simple and easy canopy was built in bucket top, and upper placement curtain shuts out the light, and makes intensity of illumination maintain 200-4500Lux, the light injury of avoiding sunburst to cause cell; Within the 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid; Be cultured to the 7th day, stop inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continues to cultivate, and to the 9th day, revolves chain Chaetoceros density and is increased to 600 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, after culture vessel is washed, adopt ", add disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate; Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine are really shredded, in 60-70.At ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.”。
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute;
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute;
Preferably, to the described reactor of selecting, minute two sterilized phases after washing, are first used high pressure nitrogen to spray sterilization, sterilization 2-3 minute; Use high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute; After sterilization, carry out again algae culture.
Useful result
Revolving the cultivation of chain Chaetoceros is to adopt Zhanjiang Aquatic Production College's formula (ammonium nitrate 5-20 milligram traditionally, potassium primary phosphate 0.5-1 milligram, ironic citrate 0.5-2 milligram, 1000 milliliters, seawater of sterilization) and adopt open cement pit to cultivate, growth is slow, easily pollute, cost is high, benefit is low, operation effort, workload is large, I adopt retention basin cultivation and in substratum, have added cow dung leach liquor now, ammonium nitrate, people's urine, urea, potassium silicate, potassium primary phosphate, fish meal, triacontanol price quote, chondroitin sulfate, marine yeast, bread fresh yeast, tyrosine, Vltra tears, L-arginine, medical stone powder, 920 plant growth substances, vitamin(e) S-3 solution, M-F trace element solution, soil extract, ooze leach liquor, Trypsin, Tutofusin tris, the nutritive ingredients such as oxyacetic acid, not only can low-cost processes diary farm ight soil, utilization of waste material, turn waste into wealth, and can obtain important food value revolve chain Chaetoceros, achieve many things at one stroke, really be the ingenious utmost point, simply fantastic.Fertilizer and inorganic fertilizer mix use, and nutrition is more comprehensive, balanced, easy to operate, and cost is low, and high efficiency is revolved the chain Chaetoceros speed of growth and output and significantly improved, and culture cycle shortens, and output improves 320%.
Accompanying drawing explanation
Fig. 1 is for revolving chain Chaetoceros morphological structure figure;
Wherein Reference numeral is: 1 jiao of hair, 2 cell wallss, 3 chromatoplasts;
Fig. 2 is for utilizing diary farm ight soil cultivation to revolve chain angle hair algae method figure;
Wherein Reference numeral is: 4 vapor pipes, 5 electricity-saving lamps, 6 culture tank, 7 algae liquid, 8 water taps, 9 gas exhausting stones, 10 ladders, 11 gas-filled valves.
Embodiment
1) cow dung leach liquor preparation method: get 50 kilograms of fresh cow dungs, add 3 kilograms, wheat bran, 1 kilogram of Semen Maydis powder, 4 liters, tap water, fully stir, pile coniform, spontaneous fermentation 15 days, rear stand lamellar, under sunlight, be exposed to the sun 3 days, after get 3 kilograms and put into bucket, add 10 liters of distilled water, fully stir evenly, standing, 100 order silk cover filterings after 48 hours, get filtrate heating, boil 45 minutes, kill bacterium wherein, mould, virus etc., cooling, precipitate 24 hours, get supernatant liquor, obtain cow dung leach liquor, pack grinding port plug reagent bottle into, sealing, be placed in 4 ℃ of refrigerators or freezer standby,
2) soil extract preparation method: get loose in the woods, be rich in 1 kilogram of organic upper layer of soil, add 1000 milliliters of pure water, boil 60 minutes, in the dark place 2 days, filter, 600 milliliters of filtrates add 400 milliliters of uses of pure water;
3) ozonization seawater method: get seawater, absorbent cotton filters, and passes into ozonization; It is 3500 milli Grams Per Hours that ozonizer used produces ozone amount, and every cubic metre of seawater fills ozone 30 minutes, charges rear natural aeration 90 minutes, allows too much ozone in water body evaporate, can be in order to support algae;
4) peracetic acid disinfectant liquid and preparation method thereof and application notice: the former liquid hold-up 35% of commercially available Peracetic Acid (by weight), colourless liquid, has intense stimulus smell.During use, apply clean tap water and be diluted to 0.2% solution, soak water butt inwall 2 hours.These product have intense stimulus effect to eyes, skin, mucous membrane and the upper respiratory tract, headache, nauseating and inflammable, and tool explosivity, severe corrosive, strong and stimulating, can cause human body and burn, and is heated to 100 ℃ of i.e. fierce decomposition, meets fiery or be heated, be subject to shake all can detonate.Contact with reductive agent, promotor, organism, combustiblematerials etc. vigorous reaction can occur; Not directly catch tactile, during operation, should be with rubber gloves, mouth mask and glasses, metal is corrosive, be not useable for the sterilization of metallic weapon; The long-term placement of stoste can be decomposed, and notes the quality guaranteed period, should store in cool dark place in plastic tank and preserve;
5) the chain Chaetoceros that revolves of inoculation use is purified (this area ordinary method, as long as can realize purification, Kai Shi extraction method for example) in advance, and algae kind is in eugonic exponential phase of growth when guaranteeing to inoculate;
6) various nutritive salt will add by the order providing in formula, to avoid chemical reaction;
7) various inorganic nutrient salts and VITAMIN need be made in advance mother liquor and be placed in 4 ℃ of refrigerator cold-storages and save backup, and storage period is not over 45 days;
8) medical stone powder is medical stone powder goods, by medical stone raw ore, through screening, is pulverized, and is processed into floury, and granular size can be passed through 200 mesh sieve thin,tough silk;
9) 920 plant growth substances are white crystalline powder, are difficult for being dissolved in water, can first with alcohol, fully dissolve (general every gram dissolves more than 1 hour with 50 ml ethanols or 60 degree white wine), then be watered dilution by desired concn;
10) ooze extracts liquid and preparation method thereof: get the more fertile black ooze of what not again, with 1 part of mud, add 2 parts of fresh water, stir, standing 1-2 minute, get upper strata mud in aluminum pot, the amount that adds 1 gram of NaOH by every 1000 milliliters of mud adds NaOH, boils 25 minutes.While boiling, need constantly stir, boil and within latter standing 24 hours, get supernatant liquor, sealing, is placed in 4 ℃ of refrigerators or freezer standby;
11) M-F trace element solution is joined method: 0.2 gram of boric acid, and 0.2 gram of Manganous chloride tetrahydrate, 0.2 milligram of molybdic oxide, 0.02 gram, copper sulfate, 0.02 gram, zinc sulfate, 0.02 gram of cobalt chloride, is dissolved in 1000 ml distilled waters, finally splashes into a vitriol oil, shakes up and get final product;
12) vitamin(e) S-3 solution is joined method: 50 milligrams of thiamine salt hydrochlorates, and 10 milligrams of Nicotinicum Acidums, 10 milligrams of calcium pantothenate, 1.0 grams of para-amino benzoic acid, 0.1 gram of vitamin H, 0.5 gram of inositol, folic acid 0.2 microgram, between chest, pyridine is 0.3 gram, 1000 milliliters of distilled water;
13) people urinates preparation method: get people and urinate 1000 milliliters, flask boils rear cooling standby;
14) pharmaceutical chemicals purity used all needs chemical pure rank.
The cow dung leach liquor of take is main raw material revolves a chain angle hair algae culture medium, fills a prescription as follows:
150 milliliters of cow dung leach liquors, 4 milliliters, ammonium nitrate, people urinates 1 milliliter, 2 milligrams, urea, 0.5 milligram of potassium silicate, 5 milligrams of potassium primary phosphates, 4 milligrams of fish meal, 0.2 milligram of triacontanol price quote, 2 milligrams of chondroitin sulfates, 0.5 milligram of marine yeast, 1 milligram of bread fresh yeast, 0.8 milligram, tyrosine, 0.6 milligram of Vltra tears, 1 milligram of L-arginine, 6 milligrams of medical stone powders, 920 plant growth substance 4 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 1.2 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 6 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 1 milliliter of oxyacetic acid, 1000 milliliters, the pure seawater of ozonization.
Utilization utilizes diary farm ight soil leach liquor to revolve a chain angle hair algae method for main raw material cultivation, and concrete steps are as follows:
Culture vessel is volume 3m
3water butt, market is on sale, and food grade high-density polyethylene material is made, thickening, seamless integral structure, sturdy and durable, shock resistance, water white transparency, has good light permeability, in order to revolving chain Chaetoceros, carries out photosynthesis.Have a water tap near bottom, a circular lid is arranged at bucket top, above has the silk of revolving, rotatable and seal, and lid is stamped 3 holes, and gas-filled valve is inserted in a hole, and vapor pipe is inserted in another hole, and gas-filled valve and vapor pipe are all Glass tubings, 6 millimeters of internal diameters.Also have a hole to penetrate a plain conductor, wire end connects a 40w electricity-saving lamp.Because bucket is higher, there is a ladder on bucket side, convenient operation up and down.Water butt is washed, with distilled water rinse 3 times, pack 2 tons of substratum claimed in claim 1 into afterwards, inflation mixes 5 minutes, selects vigorous, the free of contamination inoculation of chain Chaetoceros, the inoculum density 4 * 10 of revolving of vitality
4cell/mL; On lid, insert two clean Glass tubings, one longer, 100 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation (effect of gas exhausting stone is to form very little bubble, avoids the wave of air pocket or rolling to smash frustule); Another root is shorter, and 35 centimetres, lid is exposed at two ends, in order to exhaust; Two same thicknesses of Glass tubing; With clean scotch tape, two Glass tubings are fixed; Long Glass tubing upper end materials pipe is connected with aerator pump, is filled with mixed gas, and mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%; Continuous charge, aeration quantity should not be too large, with frustule, can not float; Outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-8000Lux; Opening electricity-saving lamp night throws light on.Cultivate first 5 days, a simple and easy canopy was built in bucket top, and upper placement curtain shuts out the light, and makes intensity of illumination maintain 200-4500Lux, the light injury of avoiding sunburst to cause cell, within the 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid, be cultured to the 7th day, stop inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continues to cultivate, and to the 9th day, revolves chain Chaetoceros density and is increased to 600 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, after culture vessel is washed, adopt ", add disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate, described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.”。
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute;
Preferably, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute;
Preferably, to the described reactor of selecting, minute two sterilized phases after washing, are first used high pressure nitrogen to spray sterilization, sterilization 2-3 minute; Use high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute; After sterilization, carry out again algae culture.
It is commercially available that chain Chaetoceros polyethylene water storing tank used is revolved in cultivation.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (6)
1. the cow dung leach liquor of take is main raw material revolves a chain angle hair algae culture medium, it is characterized in that formula is as follows:
150 milliliters of cow dung leach liquors, 4 milliliters, ammonium nitrate, people urinates 1 milliliter, 2 milligrams, urea, 0.5 milligram of potassium silicate, 5 milligrams of potassium primary phosphates, 4 milligrams of fish meal, 0.2 milligram of triacontanol price quote, 2 milligrams of chondroitin sulfates, 0.5 milligram of marine yeast, 1 milligram of bread fresh yeast, 0.8 milligram, tyrosine, 0.6 milligram of Vltra tears, 1 milligram of L-arginine, 6 milligrams of medical stone powders, 920 plant growth substance 4 international unit, 1 milliliter of vitamin(e) S-3 solution, 1 milliliter of M-F trace element solution, 1.2 milliliters of soil extracts, 1.2 milliliters of ooze leach liquors, 6 milligrams of Trypsins, 0.5 milligram of Tutofusin tris, 1 milliliter of oxyacetic acid, 1000 milliliters, the pure seawater of ozonization.
2. utilize diary farm ight soil leach liquor to revolve a chain angle hair algae method for main raw material cultivation, it is characterized in that concrete steps are as follows:
Culture vessel is volume 3m
3water butt, market is on sale, food grade high-density polyethylene material is made, thickening, seamless integral structure, sturdy and durable, shock resistance, water white transparency, there is good light permeability, in order to revolving chain Chaetoceros, carry out photosynthesis, near bottom, there is a water tap, one circular lid is arranged at bucket top, on have the silk of revolving, rotatable and seal, lid is stamped 3 holes, gas-filled valve is inserted in a hole, vapor pipe is inserted in another hole, gas-filled valve and vapor pipe are all Glass tubings, 6 millimeters of internal diameters, also have a hole to penetrate a plain conductor, wire end connects a 40w electricity-saving lamp, because of bucket higher, there is a ladder on bucket side, convenient operation up and down, water butt is washed, after use distilled water rinse 3 times, pack 2 tons of substratum claimed in claim 1 into, inflation mixes 5 minutes, selection vitality is vigorous, the free of contamination chain Chaetoceros that revolves is inoculated, inoculum density 4 * 10
4cell/mL, on lid, insert two clean Glass tubings, one longer, 100 centimetres, 15 centimetres, lid is exposed on top, the logical charge tube in lower end is connected with gas exhausting stone, in order to inflation, the effect of gas exhausting stone is to form very little bubble, avoid the wave of air pocket or rolling to smash frustule, another root is shorter, 35 centimetres, lid is exposed at two ends, in order to exhaust, two same thicknesses of Glass tubing, with clean scotch tape, two Glass tubings are fixed, long Glass tubing upper end materials pipe is connected with aerator pump, be filled with mixed gas, mixed gas composition and volume ratio are air: pure carbon dioxide: ozone: ammonia=92%: 6%: 1%: 1%, continuous charge, aeration quantity should not be too large, can not float outdoor cultivation, solar light irradiation, 25 ± 2 ℃ of optimum tempss, optimum illumination intensity 4000-8000Lux with frustule, opening electricity-saving lamp night throws light on, cultivate first 5 days, a simple and easy canopy is built in bucket top, upper placement curtain shuts out the light, and makes intensity of illumination maintain 200-4500Lux, the light injury of avoiding sunburst to cause cell, within the 6th day, remove curtain, stronger illumination is conducive to the growth and breeding of cell and synthesizing of high unsaturated fatty acid, is cultured to the 7th day, stops inflation, take away lid, supplement 100 liters of fresh cultures, to make up the moisture evaporating, cover lid, inflation, continue to cultivate, to the 9th day, revolve chain Chaetoceros density and be increased to 600 * 10
4cell/mL, stops cultivating, and from the water tap of bottom, emits algae liquid, after culture vessel is washed, adds disinfection of Chinese drug liquid disinfectant 30 minutes, after enter next round and cultivate,
Described Traditional Chinese medicine disinfectant is comprised of the material of following weight ratio: the Chinese medicinal materials that adds following weight part in every 100 part of 85% ethanol: 30～50 parts of Root of Indigowoad, 15～25 parts of radix bupleuri, 15～25 parts of peppermints, 5～15 parts of cape jasmines, 5～15 parts of tarragons, disinfection of Chinese drug liquor manufacture method: by fresh Root of Indigowoad, radix bupleuri, mentha leave, tarragon, mountain Cape jasmine really shreds, at 60-70 ℃, dry, with Chinese medicine material crushing machine, be crushed to respectively 70 orders more standby, with table balance, take respectively 120 grams of the Root of Indigowoad drying and pulverize, 60 grams of radix bupleuri, 60 grams of mentha leavves, 30 grams of tarragons, 30 grams of mountain Cape jasmine fruits, be placed in after the abundant mixing of Erlenmeyer flask, add 1000 milliliter of 85% ethanol, soak 48 hours, 200 silk cover filterings, clear liquid is staticly settled to 36 hours, skim liquid level floater, get clear liquid at the middle and upper levels, be compound Chinese medicine disinfection solution, be sealed in black reagent bottle, labelled, be placed in 4 ℃ of refrigerators standby.
3. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses ultraviolet lamp disinfection, sterilization 10-20 minute.
4. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high pressure nitrogen to spray sterilization, sterilization 5-10 minute.
5. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, the described reactor to selecting, carries out disinfection after washing, and uses high-pressure carbon dioxide to spray sterilization, sterilization 5-6 minute.
6. a kind of phosphate fertilizer plant's waste water that utilizes according to claim 2 cultivates half-naked boat-shaped algae method, it is characterized in that, to the described reactor of selecting, minute two sterilized phases after washing, first use high pressure nitrogen to spray sterilization, sterilization 2-3 minute, is used high-pressure carbon dioxide to spray sterilization, sterilization 2-3 minute, carries out algae culture again after sterilization.
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