CN104087645A - Solid fermentation ginseng flower and preparation method thereof - Google Patents
Solid fermentation ginseng flower and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a solid fermentation ginseng flower and a preparation method thereof. The preparation method comprises the following steps: uniformly placing sterilized ginseng flowers on the surface of an SL solid culture medium without a carbon source, spraying a lactobacillus plantarum seed solution, and carrying out sealed fermentation at 35 DEG C. According to the preparation method, an SL solid culture medium C-Y-1 without the carbon source is adopted; when the fermentation is carried out for 1 day, the content of ginseng total saponins is 160.230 mg/g, is increased by 76.215 mg/g compared with that of a negative tested product and increased by 1.91 times, the content of Rg3 is 0.076 mg/g, and the Rg3 is not detected in the negative tested product; when the fermentation is carried out for 3 days, the content of Rh2 is increased by 0.612 mg compared with that of the negative tested product and increased by 2.34 times; when the fermentation is carried out for 1 day, the content of F1 is increased by 0.156 mg compared with that of the negative tested product and increased by 1.2 times; when the fermentation is carried out for 7 days, the content of F2 is increased by 0.106 mg compared with that of the negative tested product and increased by 3.04 times.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, specifically a kind of solid fermentation Ginseng Flower and preparation method.
Background technology
Solid fermentation (solid-state fermentation) can be defined as microorganism conventionally at the process of growth and the bioprocesses that do not contain or contain hardly free water culture medium.
Microorganism strains for solid fermentation is grown, is bred on the substratum that almost there is no free water, and produces meta-bolites, and its process belongs to biological species reaction.According to the object of fermentation, can be summarized as two kinds of base types: 1, taking the grown cultures of microorganism or spore as object, 2, taking the production of enzyme digestion reaction and meta-bolites as object.But some fermenting process has comprised microbial growth cultivation, also comprise the production of enzyme digestion reaction and meta-bolites, as the cultivation of the cultivation of distiller's yeast and soy sauce, meter Qu.
Microorganism strains for solid fermentation is grown, is bred on the substratum that almost there is no free water, and produces meta-bolites, and its process belongs to biological species reaction.According to the object of fermentation, can be summarized as two kinds of base types:
taking the grown cultures of microorganism or spore as object;
taking the production of enzyme digestion reaction and meta-bolites as object.But some fermenting process has comprised microbial growth cultivation, also comprise the production of enzyme digestion reaction and meta-bolites, as the cultivation of the cultivation of distiller's yeast and soy sauce, meter Qu.
Ginseng (
panax ginsengc. A. Meyer) be Araliaceae (Araliacede) Panax (Panax L.) plant, be one of well-known " northeast Triratna ", the traditional rare Chinese herbal medicine of China, has won fame both at home and abroad especially.The physician of the Ming Dynasty of China greatness, pharmacy man LI Shi-Zhen, in its epic monumental work Compendium of Material Medica one book, have been done detailed introduction to effect of ginseng, have established the good reputation of ginseng " kings of hundred grass ".Ginseng grows in that day and night temperature is little more, height above sea level is the softwood forest of 500～1100 meters or the hillside of mixed forest in, its main producing region is mainly distributed in 40 °～45 ° (N), between 117.5 °～134 ° (E)
[6], be mainly distributed in the area, mountain region of three provinces in the northeast of China and northern Hebei in China, especially taking Mountain area, Changbai as main producing region.China is one of country the earliest of research ginseng, in the pharmacology monograph Shennong's Herbal of earliest extant, recorded in detail ginseng and had " tonifying the five internal organs, peace spirit, determine soul, only palpitation with fear, except perverse trend, eyesight-improving intelligence-developing, take to make light of one's life by commiting suicide for a long time and prolong life " effect
[7].Modern pharmacology research shows, ginseng have reinforce vital energy, multiple arteries and veins takes off admittedly, reinforce the spleen to benefit the lung, promoting production of body fluid and inducing sedation of the mind effect, ginseng has anti-arrhythmia, anti-hemolysis, adjusting immunity system, regulates many pharmacological activities such as central nervous system simultaneously
[8].The chemical composition more complicated containing in ginseng, except containing ginsenoside, in ginseng, also contain the activeconstituents of multiple non-saponin(e, as contain carbohydrate (monose, oligosaccharides, oligose, polysaccharide etc.), amino acid, lipid acid, brass, VITAMIN (VB1, VB2, VB12, nicotinic acid, pantothenic acid etc.) and volatilization wet goods component.Along with deepening continuously of modern pharmacology, pharmacokinetic in recent years, the pharmaceutical use of ginseng and health-care effect thereof have further obtained certification, constantly rise violently in price and the status of ginseng, nowadays ginseng, not only as the rare Chinese herbal medicine of China, has obtained the people of the world's extensive accreditation especially.
Ginsenoside has good pharmacologically active and health-care effect, shows that through pharmaceutical research ginsenoside has good pharmacological activity for cardiovascular and cerebrovascular, central nervous system, immunity system, Digestive tract etc.And very active for the research of monomer ginsenoside pharmacological activity, the particularly very micro-rare ginsenoside of some content, has high pharmaceutical use.
F group ginsenoside has provide protection to cerebral ischemia, and its mechanism is for reducing apoptosis and increasing vegf expression.
Due to the content difference of different monomer ginsenosides in ginseng, we are referred to as main ginsenoside higher a part of content ginsenoside, as ginsenoside-Ra
1, Rb
1, Rb
2, Rd, Re, Rf, Rg
1deng; Ginsenoside-Rg
2, Rg
3, Rg
5, Rh
1, Rh
2, Rh
3, Rh
4equal size is extremely low, is called as rare ginsenoside.Show through modern pharmacology research, rare ginsenoside, with respect to main ginsenoside, has better pharmacological activity conventionally.The anti-tumor activity of ginsenoside aglycon is the strongest, and its strong and weak rule is: oleanolic acid type ﹥ panoxadiol type ﹥ panoxatriol type; And along with the increasing of glucosides number, its anti-tumor activity weakens successively: aglycon ﹥ bioside ﹥ tri-glucosides ﹥ tetrose glycosides; Ginsenoside anti-tumor activity is subject to the impact of C-20 site hydroxyl configuration, and its strong and weak order is: 20 (S) ﹥, 20 (R).Most of natural ginsenosides can not directly be absorbed by human body, are difficult to directly bring into play drug effect; The utilization that just can be absorbed by the body after intestinal microflora de-glycosylation after being hydrolyzed under acidic conditions under one's belt of natural ginseng saponin(e; The secondary saponin and the aglycon that produce through de-glycosylation processing have higher bioavailability and pharmacologically active.But rare secondary ginsenoside and the content of aglycon in root, stem, leaf, bud and the fruit of the Araliaceaes such as ginseng, Radix Panacis Quinquefolii, pseudo-ginseng are very micro-, directly extract very difficulty.
Ginseng is outputed yellowish green little Hua in early summer time, and Ginseng Flower is known as again " the refreshing showy flowers of herbaceous plants ": ginseng grows to 3 years sides blooms, and each strain ginseng one is only out a little Hua, and 60 jin of ginsengs only can be adopted and to obtain 50g ginseng flower; Ginseng Flower is precious, have the title of " green gold ", its refreshing oneself, all many-sides such as step-down, hypoglycemic, reducing blood-fat, anticancer, regulating gastointestinal function have very outstanding pharmacologically active and health-care effect.
Different sites in ginseng plant, there were significant differences for the total glycosides content of its ginseng, shows after deliberation, and the total glycosides content of ginseng in Ginseng Flower is the highest, and total glycosides content in fruit, root, cauline leaf reduces successively.Monomer ginsenoside-Rb, Rc and Rg
1underground part (ginseng main root, fibrous root, reed head) content at ginseng is higher; Monomer panax saponin-Re is higher at over-ground part (bud, leaf and the fruit) content of ginseng; The PPD content of ginseng underground part is a little more than part on the ground; The PPT content of ginseng over-ground part is a little more than underground part.Lee measures to the high total glycosides of ginseng, aglycon and main saponin content to different sites in ginseng plant, finds that the panax saponin-Re content in Ginseng Flower is the highest.
Present stage focuses mostly in the research to ginseng for the report of Microbiological Transformation of Ginsenoside (comprising liquid fermenting and solid fermentation), and rarely found about the report of the microbial transformation aspect at other positions such as Ginseng Leaf, ginseng flower bud and ginseng fruit, the particularly research of microbial solid fermentation Ginseng Flower, domestic and international rare report.
Summary of the invention
Object of the present invention is intended to improve the content of Ginseng Flower total saponins and rare saponin(e, and a kind of solid fermentation Ginseng Flower and preparation method are provided.
A kind of solid medium C-Y-1, its component is: peptone 9.5-10.5g, yeast powder 4.7-5.2g, ammonium acetate 1.8-2.1g, trisodium citrate 24-25g, magnesium sulfate 0.361-0.363g, manganous sulfate 0.19-2.1g, ferrous sulfate 0.029-0.031g, potassium primary phosphate 5.8-6.2g, polyoxyethylene sorbitan monooleate 0.9-1.1mL, agar 18g, be dissolved in 1L water, regulate pH 5.5-6.2.
Described peptone 10g, yeast powder 5g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulates pH 5.85.
A kind of solid fermentation Ginseng Flower, it is by the Ginseng Flower of sterilizing, is evenly placed in without on carbon source SL solid culture primary surface spray plant lactobacillus seed liquor, 30-35 DEG C of fermentation 1-7 days;
Described is above-mentioned a kind of solid medium C-Y-1 without carbon source SL solid medium;
Described Ginseng Flower comprises Radix Panacis Quinquefolii flower.
A kind of solid fermentation Ginseng Flower preparation method:
1) plant lactobacillus is seeded in SL solid medium, picking list bacterium colony access SL liquid nutrient medium carries out activation treatment, obtains seed liquor.
2) Ginseng Flower after sterilizing is evenly placed on the C-Y-1 solid culture primary surface of thickness 0.5-2.0cm to the plant lactobacillus seed liquor of spray described in step 1), 35 DEG C of sealed fermentings of temperature.
3) pick up the Ginseng Flower of fermentation from solid medium;
Described SL liquid nutrient medium is C-Y liquid nutrient medium, its component is: peptone 10g, yeast powder 5g, glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 5.85.
A kind of solid fermentation Ginseng Flower, it is to be made up of aforesaid method, step 2) described fermentation, fermentation time is 1 day.
A kind of solid fermentation Ginseng Flower, it is to be made up of aforesaid method, step 2) described fermentation, fermentation time is 3 days.
A kind of solid fermentation Ginseng Flower, it is to be made up of aforesaid method, step 2) described fermentation, fermentation time is 7 days.
The invention provides a kind of solid fermentation Ginseng Flower, it is by the Ginseng Flower of sterilizing, is evenly placed in without on carbon source SL solid culture primary surface spray plant lactobacillus seed liquor, 35 DEG C of sealed fermentings; Adopt without carbon source SL solid medium C-Y-1; Ferment 1 day time, the total glycosides content of its ginseng 160.230mg/g has increased 76.215mg/g compared with negative trial-product, has improved 1.91 times, Rg
3content is 0.076mg/g, and negative trial-product does not detect Rg
3; Ferment 3 days time, Rh
2content has increased 0.612mg compared with negative trial-product, has improved 2.34 times; Ferment 1 day time, F
1content has increased 0.156mg compared with negative trial-product, has improved 1.2 times; Ferment 7 days time, F
2content has increased 0.106mg compared with negative trial-product, has improved 3.04 times.
Ginseng Flower outward appearance after fermentation is complete, without broken; After fermentation, separate easily, simply, facilitate the extraction of ginsenoside; Color is obviously turned black, and with strong lactic acid smell (seeing Fig. 3,4).
Brief description of the drawings
Fig. 1 lactobacillus plantarum strain produces the qualification of beta-glucosidase.
Fig. 2 plant lactobacillus is at S-L substratum (left side) and C-Y substratum (right side) upgrowth situation of 24 hours.
Fig. 3 is the photo of Ginseng Flower before solid medium top fermentation.
Fig. 4 is the photo of Ginseng Flower after solid medium top fermentation.
Embodiment
embodiment 1 prepares substratum
C-Y substratum: peptone 10g, yeast powder 5g, glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 5.85.
C-Y-1 substratum: peptone 10g, yeast powder 5g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 5.85.
C-Y-2 substratum: glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 6.05.
Above-mentioned substratum is liquid nutrient medium, and solid medium adds agar 18g again.
SL substratum.
the qualification that embodiment 2 plant lactobacilluss produce beta-glucosidase
In Bechtop, carry out aseptic technique, by the Vitamin C2-R2A solid medium pour plate preparing, after substratum cooled and solidified, with connecing collarium picking plant lactobacillus (Lactobacillus plantarum), be coated on a dull and stereotyped side, opposite side is as space management, flat board is moved into from Bechtop to biochemical cultivation case, it is 37 DEG C that culture temperature is set, static cultivation 48 hours, after taking-up is dull and stereotyped, observation substratum is coated with the colour-change of a side and space management, and result is coated with a side and has become black (see figure 1).
the selection of embodiment 3 seed culture mediums
By the substratum pour plate configuring, after substratum cooled and solidified, with connecing collarium picking plant lactobacillus, adopt 4 ride vaccination ways, bacterial strain is inoculated in respectively in substratum, moves in biochemical cultivation case, it is 37 DEG C by temperature that cultivation is set, static cultivation, after 48 hours, is observed the growth characteristic of bacterial strain.Plant lactobacillus is inoculated into after C-Y substratum, and the upgrowth situation of bacterium colony is obviously better than SL substratum, as shown in Figure 2.
solid fermentation on embodiment 4 Ginseng Flower C-Y solid mediums
1, plant lactobacillus is seeded in C-Y solid medium, picking list bacterium colony access C-Y liquid nutrient medium carries out activation treatment, and the bacterium liquid after 3 times is as fermentation seed liquid (C-Y liquid nutrient medium) repeatedly.
Configuration solid fermentation substratum: 10 groups of C-Y solid fermentation substratum, every group do 3 parallel, every group of substratum: 3 × 25mL=75mL, needs C-Y solid fermentation substratum: 75mL × 10=750mL altogether; Be divided in glass tissue culture bottle (240mL), high pressure steam sterilization (115 DEG C, 30min) is stand-by.Every group of Ginseng Flower: 3 × 1g=3g, needs Ginseng Flower: 3 × 10g=30g altogether, stand-by after quick high-pressure steam sterilizing and ultraviolet radiation sterilizing.
Aseptic technique, is laid in Ginseng Flower on the surface of C-Y solid fermentation substratum; Take out the plant lactobacillus bacterium liquid that activate, accurately draw appropriate bacterium liquid with pipettor, point 2 even sprays on Ginseng Flower surface, move in biochemical cultivation case static cultivation after screwing bottle cap; Pick up the Ginseng Flower of fermentation from solid medium.
solid fermentation in embodiment 5 Ginseng Flower C-Y-1 solid mediums
1, plant lactobacillus is seeded in C-Y solid medium, picking list bacterium colony access C-Y liquid nutrient medium carries out activation treatment, and the bacterium liquid after 3 times is as fermentation seed liquid (C-Y liquid nutrient medium) repeatedly.
2,10 groups of configuration C-Y-1 type solid mediums, every group do 3 parallel, every group of substratum: 3 × 25mL=75mL, needs C-Y-1 type solid medium: 75mL × 10=750mL altogether; Be divided in glass tissue culture flasks (240mL), high-temp steam sterilizing is stand-by; Every group of Ginseng Flower (life in 5 years is dried Ginseng Flower, originates from county of Jilin Ji'an): 3 × 1g=3g, needs Ginseng Flower: 3 × 10g=30g altogether, stand-by after the radiation sterilization of quick high-temp steam sterilizing combined uv.
3, aseptic technique, the substratum configuring is toppled in glass tissue culture flasks, after substratum cooled and solidified, Ginseng Flower is evenly laid in to solid culture primary surface, form very thin one deck, but without compression, make to leave between Ginseng Flower and substratum certain gap, the spray of bacterium liquid after being convenient to, making has the space (see figure 2) contacting between bacterium liquid and Ginseng Flower, substratum, the bacterium liquid spray having activated (is accurately drawn to bacterium liquid 0.5mL with pipettor) on the surface of Ginseng Flower, spray 2 times.
4, move in biochemical cultivation case static cultivation after screwing bottle cap.
5, pick up the Ginseng Flower of fermentation from solid medium.
solid fermentation in embodiment 6 Ginseng Flower C-Y-2 solid mediums
1, plant lactobacillus is seeded in C-Y solid medium, picking list bacterium colony access C-Y liquid nutrient medium carries out activation treatment, and the bacterium liquid after 3 times is as fermentation seed liquid (C-Y liquid nutrient medium) repeatedly.
2,10 groups of configuration C-Y-2 type solid mediums, every group do 3 parallel, every group of substratum: 3 × 25mL=75mL, needs C-Y-2 type solid medium: 75mL × 10=750mL altogether; Be divided in glass tissue culture flasks (240mL), high-temp steam sterilizing (115 DEG C, 30min) is stand-by.Every group of Ginseng Flower: 3 × 1g=3g, needs Ginseng Flower: 3 × 10g=30g altogether, stand-by after the radiation sterilization of quick high-temp steam sterilizing combined uv.
3, aseptic technique, the substratum configuring is toppled in glass tissue culture flasks, after substratum cooled and solidified, Ginseng Flower is evenly laid in to solid culture primary surface and (forms very thin one deck, but without compression, make to leave between Ginseng Flower and substratum certain gap, the spray of bacterium liquid after being convenient to, making has the space contacting between bacterium liquid and Ginseng Flower, substratum), the bacterium liquid spray having activated (is accurately drawn to bacterium liquid 0.5mL with pipettor on the surface of Ginseng Flower, spray 2 times, need altogether 1mL bacterium).
4, move in biochemical cultivation case static cultivation after screwing bottle cap.
5, pick up the Ginseng Flower of fermentation from solid medium.
the extraction of embodiment 7 ginsenosides
Adopt ultrasonic extraction to carry out the extraction of ginsenoside, concrete operation step is as follows:
1, the Ginseng Flower after drying treatment is converted into filter paper packet with aseptic neutral filter paper, puts into 50mL triangular flask, adds 20mL methyl alcohol, is tamping bottleneck with plastics film, triangular flask is put into ultrasonic cleaner, supersound process 20 minutes.
2, the solution extracting, till thin-layer chromatography detects without saponin(e reaction, discards filter paper packet, and united extraction liquid is transferred in beaker, 80 DEG C of water bath method extracting solutions.
3, take out beaker, add 20mL distilled water to redissolve, shift solution to separating funnel.
4, in separating funnel, add isopyknic ethyl acetate to carry out removal of impurities processing (removing alcohol dissolubility impurity), fully static solution layering, the collection lower floor solution treated after concussion; Re-treatment 4 times.
5, be again placed in separating funnel through 4 solution after treatment, add isopyknic water-saturated n-butanol solution to carry out removal of impurities processing (removing water-soluble impurity), fully concussion is processed, and after solution layering, collects upper solution; Re-treatment 4 times, combining extraction liquid.
6, extraction liquid is transferred in beaker, 80 DEG C of water bath method extraction liquids.
7, accurate measuring 10mL methyl alcohol redissolves, and packs in 10mL sample plasma bottle, to be measured.
the detected result of the total glycosides content of embodiment 8 ginseng
According to the report of domestic and international existing document and 2010 version " Chinese Pharmacopoeia " (one) about the relevant elaboration of the total glycosides content assaying method of ginseng, adopt ultraviolet-visible spectrophotometry to measure the total glycosides content of ginseng.
1, total glycosides assay result (in table 1) in the Ginseng Flower sample of sterilising treatment front and back.
Result shows the prolongation along with the high-temp steam sterilizing time, larger on the impact of the total glycosides content of ginseng in sample, and ultraviolet radiation sterilization affects the content of total glycosides hardly.Quick high-temp steam sterilizing (115 DEG C, 6 minutes) combined uv radiation sterilization (40W, 30 minutes), be controlled within 1%, and sterilising effect is good for the variation of the total glycosides content of ginseng.
2, Ginseng Flower is in 24,48,72 hours measurement results (in table 2) of C-Y solid medium top fermentation.
Find afterwards by analysis: the difference of fermentation time, leavening temperature, fermentation pH, 4 kinds of factors of inoculum size does not have statistical significance, and the content of ginsenoside of different experiments group changes little.
3, Ginseng Flower is in 24,48,72 hours measurement results (in table 3) of C-Y-1 solid medium top fermentation.
A ﹥ D ﹥ B ﹥ C, the difference of A, D two factors has statistical significance, and the difference of A factor is extremely remarkable, and the optimum zymotechnique of plant lactobacillus in C-Y-1 type substratum is: A
1b
2c
2d
1, that is: fermentation time (24h), leavening temperature (35 DEG C), fermentation pH(5.85), inoculum size (4%).
4, Ginseng Flower is in 24,48,72 hours measurement results (in table 4) of C-Y-2 solid medium top fermentation.
A ﹥ D ﹥ B ﹥ C, the difference of A, D two factors has statistical significance, the significant difference of A factor, the optimum zymotechnique of plant lactobacillus in C-Y-2 type substratum is: A
3b
1c
3d
1, that is: fermentation time (72 hours), leavening temperature (30 DEG C), fermentation pH(6.05), inoculum size (4%).
5, Ginseng Flower is in the 10 days total glycosides assay of ginseng results of C-Y solid medium top fermentation.
Ginseng Flower and C-Y solid medium are jointly as fermentation substrate, and under optimum technological condition for fermentation, through plant lactobacillus fermentation 10 days, the variation of the total glycosides content of fermentation time and ginseng was shown in Table 5.
By compared with negative trial-product, the total glycosides content of ginseng is not remarkable.
6, Ginseng Flower is in the 10 days total glycosides assay of ginseng results of C-Y-1 solid medium top fermentation.
Ginseng Flower C-Y-1 type solid medium is jointly as fermentation substrate, at fermentation time (24 hours), leavening temperature (35 DEG C), fermentation pH(5.85), under the condition of inoculum size (4%), through plant lactobacillus fermentation 10 days, the variation of the total glycosides content of fermentation time and ginseng was shown in Table 6.
By compared with negative trial-product, the total glycosides content of ginseng is remarkable.Ferment the 1st day time, the total glycosides content of ginseng reaches maximum value 160.230mg/g, has increased 76.215mg/g compared with negative trial-product, has improved 1.91 times.
7, Ginseng Flower is in the 10 days total glycosides assay of ginseng results of C-Y-2 solid medium top fermentation.
Ginseng Flower and C-Y-2 type solid medium are jointly as fermentation substrate, at fermentation time (72 hours), leavening temperature (30 DEG C), fermentation pH(6.05), under the condition of inoculum size (4%), through plant lactobacillus fermentation 10 days, the variation of the total glycosides content of fermentation time and ginseng was in table 7.
By compared with negative trial-product, the total glycosides content of ginseng is remarkable.Ferment the 3rd day time, the total glycosides content of ginseng reaches maximum value 136.960mg/g, has increased 52.945mg/g compared with negative trial-product, has improved 1.63 times.
8, each monomer ginsenoside efficient liquid phase chromatographic analysis before and after fermentation.
By HPLC method, the content of monomer ginsenoside in tunning is measured, it the results are shown in Table shown in 8.
In C-Y-1 type substratum tunning, rare ginseng Rg
3, Rh
2, F
1, F
2the variation of content:
1) fermentation is 1 day time: Rg
3content reaches maximum value, and content is 0.076mg/g, and negative trial-product does not detect Rg
3.
2) fermentation is 3 days time: Rh
2content reaches maximum value, has increased 0.612mg compared with negative trial-product, has improved 2.34 times.
3) fermentation is 1 day time: F
1content reaches maximum value, has increased 0.156mg compared with negative trial-product, has improved 1.2 times.
4) fermentation is 7 days time: F
2content reaches maximum value, has increased 0.106mg compared with negative trial-product, has improved 3.04 times.
In C-Y-1 type substratum tunning, main ginseng Rb
1, Rb
2, Rd, Rf content variation:
1) fermentation is 1 day time: Rb
1content reaches maximum value, has increased 0.730mg compared with negative trial-product, has improved 2.38 times.
2) fermentation is 1 day time: Rb
2content reaches maximum value, has increased 0.418mg compared with negative trial-product, has improved 1.51 times.
3) fermentation is 1 day time: Rd content reaches maximum value, has increased 1.906mg compared with negative trial-product, has improved 1.62 times.
4) fermentation is 3 days time: Rf content reaches maximum value, and content is 0.302mg, and negative trial-product does not detect Rf.
While beginning from microbial transformation, ginsenoside Re, Rg
1, Rg
2content start reduce, generate new secondary ginsenoside.
The significance of difference is analyzed:
1) fermentation is 3 days time, and ginsenoside-Rf content, compared with negative trial-product, has utmost point significant difference.
2) fermentation is 1 day time, ginsenoside-Rg
3content, compared with negative trial-product, has utmost point significant difference.
3) fermentation is 1 day time, ginsenoside-Rb
1, Rg
1content compared with negative trial-product, has significant difference respectively.
4) fermentation is 3 days time, ginsenoside-Rh
2, F
1content compared with negative trial-product, has significant difference respectively.
5) fermentation is 4 days time, ginsenoside-Rb
2, Rg
2content compared with negative trial-product, has significant difference respectively.
6) fermentation is 5 days time, and ginsenoside-Rd, Re content compared with negative trial-product, have significant difference respectively.
7) fermentation is 7 days time, ginsenoside-F
2content compared with negative trial-product, has significant difference respectively.
In C-Y-2 type substratum tunning, rare ginseng Rg
3, Rh
2, F
1, F
2the variation of content:
1) fermentation is 4 days time: Rg
3content reaches maximum value, and content is 0.604mg/g, and negative trial-product does not detect Rg
3.
2) fermentation is 2 days time: Rh
2content reaches maximum value, has increased 0.508mg compared with negative trial-product, has improved 2.11 times.
3, fermentation is 10 days time: F
1content reaches maximum value, has increased 7.696mg compared with negative trial-product, has improved 10.77 times.
4, fermentation is 10 days time: F
2content reaches maximum value, has increased 0.326mg compared with negative trial-product, has improved 7.27 times.
In C-Y-2 type substratum tunning, main ginseng Rb
1, Rb
2, Rd, Rf content variation:
1) fermentation is 1 day time: Rb
1content reaches maximum value, has increased 0.326mg compared with negative trial-product, has improved 1.62 times.
2) fermentation is 9 days time: Rb
2content reaches maximum value, has increased 3.584mg compared with negative trial-product, has improved 5.36 times.
3) fermentation is 1 day time: Rd content reaches maximum value, has increased 0.576mg compared with negative trial-product, has improved 1.19 times.
4) fermentation is 9 days time: Rf content reaches maximum value, and content is 1.458mg, and negative trial-product does not detect Rf.
While beginning from microbial transformation, ginsenoside Re, Rg
1, Rg
2content start reduce, generate new secondary ginsenoside, ginsenoside-Rg
2in fermentation, the 6th day time, content slowly improves; In fermentation, the 9th day time, content is 0.810mg/g, has increased 0.184mg/g compared with negative trial-product, has improved 1.29 times.
The significance of difference is analyzed:
1) fermentation is 9 days time, and ginsenoside-Rf content, compared with negative trial-product, has utmost point significant difference.
2) fermentation is 4 days time, ginsenoside-Rg
3content, compared with negative trial-product, has utmost point significant difference.
3) fermentation is 10 days time, ginsenoside-F
1content, compared with negative trial-product, has utmost point significant difference.
4) fermentation is 10 days time, ginsenoside-F
2=content, compared with negative trial-product, has utmost point significant difference.
5) fermentation is 1 day time, ginsenoside-Rb
1content compared with negative trial-product, has significant difference respectively.
6) fermentation is 2 days time, ginsenoside-Rg
2, Rh
2content compared with negative trial-product, has significant difference respectively.
7) fermentation is 4 days time, and panax saponin-Re content compared with negative trial-product, has significant difference respectively.
8) fermentation is 9 days time, ginsenoside-Rb
2, Rd, Rg
1content compared with negative trial-product, has significant difference respectively.
The total glycosides content of ginseng:
C-Y-1 type solid medium: plant lactobacillus is during to Ginseng Flower fermentation 1 day, and the total glycosides content of its ginseng reaches maximum value 160.230mg/g, has increased 76.215mg/g compared with negative trial-product, has improved 1.91 times.
C-Y-2 type solid medium: plant lactobacillus is during to Ginseng Flower fermentation 3 days, and the total glycosides content of ginseng reaches maximum value 136.960mg/g, has increased 52.945mg/g compared with negative trial-product, has improved 1.63 times.
Rare ginsenoside-Rg
3, Rh
2, F
1, F
2the variation of content:
C-Y-1 type solid medium: plant lactobacillus ferments 1 day time to Ginseng Flower, Rg
3content reaches maximum value, and content is 0.076mg/g, and negative trial-product does not detect Rg
3; Ferment 3 days time, Rh
2content reaches maximum value, has increased 0.612mg compared with negative trial-product, has improved 2.34 times; Ferment 1 day time, F
1content reaches maximum value, has increased 0.156mg compared with negative trial-product, has improved 1.2 times; Ferment 7 days time, F
2content reaches maximum value, has increased 0.106mg compared with negative trial-product, has improved 3.04 times.
C-Y-2 type solid medium: plant lactobacillus ferments 4 days time to Ginseng Flower, Rg
3content reaches maximum value, and content is 0.604mg/g, and negative trial-product does not detect Rg
3; Ferment 2 days time, Rh
2content reaches maximum value, has increased 0.508mg compared with negative trial-product, has improved 2.11 times; Ferment 10 days time, F
1content reaches maximum value, has increased 7.696mg compared with negative trial-product, has improved 10.77 times; Ferment 10 days time, F
2content reaches maximum value, has increased 0.326mg compared with negative trial-product, has improved 7.27 times.
Claims (10)
1. a solid medium C-Y-1, its component is: peptone 9.5-10.5g, yeast powder 4.7-5.2g, ammonium acetate 1.8-2.1g, trisodium citrate 24-25g, magnesium sulfate 0.361-0.363g, manganous sulfate 0.19-2.1g, ferrous sulfate 0.029-0.031g, potassium primary phosphate 5.8-6.2g, polyoxyethylene sorbitan monooleate 0.9-1.1mL, agar 18g, be dissolved in 1L water, regulate pH 5.5-6.2.
2. a kind of solid medium C-Y-1 according to claim 1, it is characterized in that: described peptone 10g, yeast powder 5g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 5.85.
3. a solid fermentation Ginseng Flower, it is by the Ginseng Flower of sterilizing, is evenly placed in without on nitrogenous source SL solid culture primary surface, spray plant lactobacillus seed liquor, 30-35 DEG C of fermentation makes for 1-7 days.
4. a kind of solid fermentation Ginseng Flower according to claim 3, is characterized in that: described is a kind of solid medium C-Y-1 described in claim 1 or 2 without nitrogenous source SL solid medium.
5. a solid fermentation Ginseng Flower preparation method, it comprises:
1) plant lactobacillus is inoculated in SL solid medium, picking list bacterium colony access SL liquid nutrient medium carries out activation treatment, obtains seed liquor;
2) Ginseng Flower after sterilizing is evenly placed on thickness 0.5-2.0cm C-Y-1 solid culture claimed in claim 2 primary surface to the plant lactobacillus seed liquor of spray described in step 1), 35 DEG C of sealed fermentings of temperature;
3) pick up the Ginseng Flower of fermentation from solid medium.
6. a kind of solid fermentation Ginseng Flower preparation method according to claim 5, it is characterized in that: described SL liquid nutrient medium is C-Y liquid nutrient medium, its component is: peptone 10g, yeast powder 5g, glucose 20g, ammonium acetate 2g, trisodium citrate 25g, magnesium sulfate 0.362g, manganous sulfate 0.2g, ferrous sulfate 0.03g, potassium primary phosphate 6g, polyoxyethylene sorbitan monooleate 1mL, be dissolved in 1L water, regulate pH 5.85.
7. a solid fermentation Ginseng Flower, is characterized in that: it is to be made up of method claimed in claim 5, step 2) described fermentation, fermentation time is 1 day.
8. a solid fermentation Ginseng Flower, is characterized in that: it is to be made up of method claimed in claim 5, step 2) described fermentation, fermentation time is 3 days.
9. a solid fermentation Ginseng Flower, is characterized in that: it is to be made up of method claimed in claim 5, step 2) described fermentation, fermentation time is 7 days.
10. Ginseng Flower claimed in claim 3, comprises Radix Panacis Quinquefolii flower.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104544076A (en) * | 2015-01-08 | 2015-04-29 | 谢形延 | Preparation method for white radish tea |
| CN105861381A (en) * | 2016-05-17 | 2016-08-17 | 吉林农业大学 | Method for lactobacillus plantarum fermented ginseng liquid |
| WO2017125464A1 (en) | 2016-01-19 | 2017-07-27 | Schweighofer Thomas Josef | Cleaning agent comprising saponin and lactic acid bacteria |
| CN111227148A (en) * | 2020-01-15 | 2020-06-05 | 吉林农业大学 | Preparation method of ginseng flower fermented beverage |
| CN113631146A (en) * | 2019-06-14 | 2021-11-09 | 自然灵感之园有限公司 | Whole plant extract containing root fermented product of ginseng cultivated by cold wind and cosmetic composition containing the same |
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| CN101974475A (en) * | 2010-11-16 | 2011-02-16 | 湖南顺信生物科技有限公司 | Photosynthetic bacteria nitrogen-free culture medium |
| KR101366498B1 (en) * | 2013-05-06 | 2014-03-12 | (주)그린솔루션스 | Method for production of ginseng flower fermentation product with abirritation and whitening effect |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974475A (en) * | 2010-11-16 | 2011-02-16 | 湖南顺信生物科技有限公司 | Photosynthetic bacteria nitrogen-free culture medium |
| KR101366498B1 (en) * | 2013-05-06 | 2014-03-12 | (주)그린솔루션스 | Method for production of ginseng flower fermentation product with abirritation and whitening effect |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104544076A (en) * | 2015-01-08 | 2015-04-29 | 谢形延 | Preparation method for white radish tea |
| CN104544076B (en) * | 2015-01-08 | 2016-08-24 | 谢形延 | A kind of manufacture method of white turnip ginseng tea |
| WO2017125464A1 (en) | 2016-01-19 | 2017-07-27 | Schweighofer Thomas Josef | Cleaning agent comprising saponin and lactic acid bacteria |
| CN105861381A (en) * | 2016-05-17 | 2016-08-17 | 吉林农业大学 | Method for lactobacillus plantarum fermented ginseng liquid |
| CN105861381B (en) * | 2016-05-17 | 2019-07-12 | 吉林农业大学 | A kind of lactobacillus plantarum fermented ginseng liquid method |
| CN113631146A (en) * | 2019-06-14 | 2021-11-09 | 自然灵感之园有限公司 | Whole plant extract containing root fermented product of ginseng cultivated by cold wind and cosmetic composition containing the same |
| CN111227148A (en) * | 2020-01-15 | 2020-06-05 | 吉林农业大学 | Preparation method of ginseng flower fermented beverage |
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