CN104073553A - 湖北麦冬pcr鉴定试剂盒及鉴定方法 - Google Patents
湖北麦冬pcr鉴定试剂盒及鉴定方法 Download PDFInfo
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- CN104073553A CN104073553A CN201410159443.5A CN201410159443A CN104073553A CN 104073553 A CN104073553 A CN 104073553A CN 201410159443 A CN201410159443 A CN 201410159443A CN 104073553 A CN104073553 A CN 104073553A
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000003752 polymerase chain reaction Methods 0.000 title abstract 4
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- 238000012408 PCR amplification Methods 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 8
- 238000001962 electrophoresis Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 238000000246 agarose gel electrophoresis Methods 0.000 claims description 3
- 230000004087 circulation Effects 0.000 claims description 3
- 238000004925 denaturation Methods 0.000 claims description 3
- 230000036425 denaturation Effects 0.000 claims description 3
- 239000012160 loading buffer Substances 0.000 claims description 3
- 238000004153 renaturation Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 2
- 240000002948 Ophiopogon intermedius Species 0.000 claims 7
- 239000003814 drug Substances 0.000 abstract description 10
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000011161 development Methods 0.000 abstract description 2
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- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 1
- 244000248557 Ophiopogon japonicus Species 0.000 description 104
- 108020004414 DNA Proteins 0.000 description 31
- 230000009182 swimming Effects 0.000 description 18
- 239000000243 solution Substances 0.000 description 11
- 239000003550 marker Substances 0.000 description 8
- 241000234282 Allium Species 0.000 description 6
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 4
- 240000006108 Allium ampeloprasum Species 0.000 description 3
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 3
- 240000002234 Allium sativum Species 0.000 description 3
- 241000132012 Atractylodes Species 0.000 description 3
- 241000331528 Chlorophytum capense Species 0.000 description 3
- 241000935235 Fritillaria meleagris Species 0.000 description 3
- 241000234435 Lilium Species 0.000 description 3
- 235000004611 garlic Nutrition 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229940126678 chinese medicines Drugs 0.000 description 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 2
- 229960005542 ethidium bromide Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241001521474 Liriope <hydrozoan> Species 0.000 description 1
- 241001348277 Liriope spicata var. prolifera Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229930195210 Ophiopogon Natural products 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201410159443.5A CN104073553B (zh) | 2014-04-18 | 2014-04-18 | 湖北麦冬pcr鉴定试剂盒及鉴定方法 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104531838A (zh) * | 2014-11-27 | 2015-04-22 | 浙江工业大学 | 浙白术pcr鉴定试剂盒及鉴定方法 |
CN104774948A (zh) * | 2015-04-16 | 2015-07-15 | 浙江工业大学 | 河北白术pcr鉴定试剂盒及鉴定方法 |
CN107574255A (zh) * | 2016-07-04 | 2018-01-12 | 谱尼测试集团江苏有限公司 | 一种快速检测草贝母和轮叶贝母成分的方法 |
CN108315467A (zh) * | 2018-04-04 | 2018-07-24 | 杭州师范大学 | 用于阔叶山麦冬鉴别的特异性分子标记lpmi001 |
CN108588260A (zh) * | 2018-05-15 | 2018-09-28 | 浙江工业大学 | 桂郁金pcr鉴定试剂盒及鉴定方法 |
CN108624708A (zh) * | 2018-05-15 | 2018-10-09 | 浙江工业大学 | 绿丝郁金pcr鉴定试剂盒及鉴定方法 |
JP6991432B1 (ja) * | 2021-04-02 | 2022-01-12 | 中国計量大学 | ジャノヒゲ産地鑑別方法 |
-
2014
- 2014-04-18 CN CN201410159443.5A patent/CN104073553B/zh active Active
Non-Patent Citations (3)
Title |
---|
G. LI,ET AL: "SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon", 《GENETICS AND MOLECULAR RESEARCH》, vol. 11, no. 3, 31 December 2012 (2012-12-31), pages 2987 - 2996 * |
刘霞等: "湖北麦冬及其近缘种的ISSR多样性研究", 《中药材》, vol. 33, no. 7, 31 December 2010 (2010-12-31), pages 1052 - 1055 * |
陈家春等: "湖北麦冬及其近缘药用植物的随机扩增引物DNA分析", 《医药导报》, vol. 26, no. 7, 31 December 2007 (2007-12-31), pages 738 - 741 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104531838B (zh) * | 2014-11-27 | 2017-01-18 | 浙江工业大学 | 浙白术pcr鉴定试剂盒及鉴定方法 |
CN104531838A (zh) * | 2014-11-27 | 2015-04-22 | 浙江工业大学 | 浙白术pcr鉴定试剂盒及鉴定方法 |
CN104774948A (zh) * | 2015-04-16 | 2015-07-15 | 浙江工业大学 | 河北白术pcr鉴定试剂盒及鉴定方法 |
CN104774948B (zh) * | 2015-04-16 | 2017-04-12 | 浙江工业大学 | 河北白术pcr鉴定试剂盒及鉴定方法 |
CN107574255A (zh) * | 2016-07-04 | 2018-01-12 | 谱尼测试集团江苏有限公司 | 一种快速检测草贝母和轮叶贝母成分的方法 |
CN107574255B (zh) * | 2016-07-04 | 2023-07-11 | 谱尼测试集团江苏有限公司 | 一种实时荧光pcr检测草贝母和轮叶贝母成分的方法 |
CN108315467B (zh) * | 2018-04-04 | 2019-02-26 | 杭州师范大学 | 用于阔叶山麦冬鉴别的特异性分子标记lpmi001 |
CN108315467A (zh) * | 2018-04-04 | 2018-07-24 | 杭州师范大学 | 用于阔叶山麦冬鉴别的特异性分子标记lpmi001 |
CN108588260A (zh) * | 2018-05-15 | 2018-09-28 | 浙江工业大学 | 桂郁金pcr鉴定试剂盒及鉴定方法 |
CN108588260B (zh) * | 2018-05-15 | 2019-11-29 | 浙江工业大学 | 桂郁金pcr鉴定试剂盒及鉴定方法 |
CN108624708B (zh) * | 2018-05-15 | 2019-11-29 | 浙江工业大学 | 绿丝郁金pcr鉴定试剂盒及鉴定方法 |
CN108624708A (zh) * | 2018-05-15 | 2018-10-09 | 浙江工业大学 | 绿丝郁金pcr鉴定试剂盒及鉴定方法 |
JP6991432B1 (ja) * | 2021-04-02 | 2022-01-12 | 中国計量大学 | ジャノヒゲ産地鑑別方法 |
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CN104073553B (zh) | 2016-01-20 |
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Effective date of registration: 20201027 Address after: 313000 no.55-9 Huyan South Road, Shuanglin Town, Nanxun District, Huzhou City, Zhejiang Province (self declaration) Patentee after: Huzhou Duxin Technology Co.,Ltd. Address before: 310014 Hangzhou city in the lower reaches of the city of Zhejiang Wang Road, No. 18 Patentee before: ZHEJIANG University OF TECHNOLOGY |
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Effective date of registration: 20221213 Address after: Room 404, Building 18, High tech Development Zone, Nangang District, Harbin City, Heilongjiang Province, 150000 Patentee after: Harbin Baoside Biotechnology Co.,Ltd. Address before: 313000 no.55-9 Huyan South Road, Shuanglin Town, Nanxun District, Huzhou City, Zhejiang Province (self declaration) Patentee before: Huzhou Duxin Technology Co.,Ltd. |