CN104069019A - Shampoo containing hyaluronic acid - Google Patents
Shampoo containing hyaluronic acid Download PDFInfo
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- CN104069019A CN104069019A CN201410257144.5A CN201410257144A CN104069019A CN 104069019 A CN104069019 A CN 104069019A CN 201410257144 A CN201410257144 A CN 201410257144A CN 104069019 A CN104069019 A CN 104069019A
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Abstract
The invention discloses shampoo containing hyaluronic acid. The hyaluronic acid contained in the shampoo disclosed in the invention is high in yield, good in transmittance, low in protein content, high in glucuronic acid content, easy to dissolve and good in moisturizing effect, the prepared shampoo containing hyaluronic acid is good in stability, good in decontamination effect, free of toxic side effect, mild and free of stimulation to skin, good in moisturizing and hair protection effects while being used for cleaning hairs and is capable of protecting the hair from damage.
Description
Technical field
The present invention relates to biological polyoses extraction and application technical field, particularly relate to a kind of hyaluronic shampoo product that contains.
Background technology
Hair shampoo article is indispensable requisites in people's daily life, can from hair and scalp, remove surperficial greasy dirt and scurf, and hair, scalp and health of human body are had no adverse effects during use.In recent years, due to the raising of people's quality of the life, increasing of hair washing number of times, has also proposed increasing requirement to hair shampoo article.Existing shampoo function singleness, shampoo, in wash clean hair, also likely can cause negative effect to a certain degree to hair, affects hair quality, makes the nutrition leak of hair, causes hair crude, does not possess the function of hair conditioning in the time of hair washing.Need now hair shampoo article to there is the anti-dandruff effect of good decontamination, need again it to there is good abnormal smells from the patient, also need to keep the gloss of hair quality simultaneously, play the nursing role to hair.And to the moistening effect of hair, be an importance that plays nursing role.
Hyaluronic acid, referred to as HA, is by β-(1 → 3) and acid mucopolysaccharide that β-(1 → 4) glycosidic bond is formed by connecting by glucuronic acid and acetylglucosamine.It has the advantageous properties such as humectant, viscoelasticity and biocompatibility, especially has unique performance of keeping humidity, has been widely used at present the fields such as medicine, daily use chemicals, food, is one of domestic and international biochemical industry circle focus product.
Domestic have tens hyaluronic manufacturers at present, and most of Shi Yong animal tissue of these enterprises extraction method.But because raw material is limited, and the content of HA is low in raw material, simultaneously HA also coexists in biological tissue with the mucopolysaccharide such as chondroitin sulfate again, causes that its yield is low, purify difficulty, high cost, poor product quality, and is difficult to be applied to large-scale production.Along with the continuous increase of HA demand, a large amount of high-quality HA is produced in an urgent demand, and oneself can not adapt to this requirement animal tissue's extraction method.The technique fermentation period that also has at present Production by Microorganism Fermentation hyaluronic acid to adopt is long, product relative molecular weight is low, product printing opacity rate variance, protein content is high, glucuronic acid content is low.The hyaluronic form having is fibrous, not soluble.Be difficult to reach the standard that is applied to cosmetic field.
Summary of the invention
Object of the present invention is exactly to provide a kind of hyaluronic shampoo product that contains for the defect of above-mentioned existence.Hyaluronic acid yield of the present invention is high, light transmittance good; protein content is low; glucuronic acid content is high, soluble, and moistening effect is good; that prepares contains hyaluronic shampoo product to good stability; clean effect is good, has no side effect, non-stimulated to skin gentleness; in the time of hair washing, have good moisturizing hair care effect, protection hair is injury-free.
Of the present inventionly a kind ofly contain hyaluronic shampoo product technical scheme and be, this shampoo product contains hyaluronic acid, described hyaluronic acid adopt streptococcus zooepidemicus (
streptococcus zooepidemicus) the AWA008 preparation of fermenting, streptococcus zooepidemicus (
streptococcus zooepidemicus) AWA008 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, deposit number is: CGMCCNo.9111, preservation date is on 04 29th, 2014.
At present this streptococcus zooepidemicus is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and postcode: 100101, its deposit number is: CGMCCNo.9111, the Latin title of strain is
streptococcus zooepidemicus, the microorganism (strain) of ginseng certificate: AWA008, preservation date is on 04 29th, 2014.
Streptococcus zooepidemicus strain characteristics of the present invention: spherical in shape or oval, be catenation, there is pod membrane, without spore, atrichia, Gram-positive.
Described a kind of hyaluronic shampoo product that contains, comprise following composition by weight: trisodium citrate 0.2-0.8%, lauric acid 10-20%, hyaluronic acid 0.5-5%, methyl butex 0.05-0.2%, dipropylene glycol 1-10%, hydrolysis aleuronat 1-2%, potassium sorbate 0.1-0.5%, disodiumedetate 0.1-0.5%, essence 0.1-0.3%, surplus is deionized water.
Described a kind of hyaluronic shampoo product that contains, further, comprise following composition by weight: trisodium citrate 0.3-0.6%, lauric acid 12-18%, hyaluronic acid 1-3%, methyl butex 0.05-0.1%, dipropylene glycol 2-8%, hydrolysis aleuronat 1-1.5%, potassium sorbate 0.1-0.3%, disodiumedetate 0.1-0.3%, essence 0.1-0.2%, surplus is deionized water.
Described a kind of hyaluronic shampoo product that contains, preferably, comprise following composition by weight: trisodium citrate 0.5%, lauric acid 15%, hyaluronic acid 2%, methyl butex 0.08%, dipropylene glycol 5%, hydrolysis aleuronat 1.2%, potassium sorbate 0.2%, disodiumedetate 0.2%, essence 0.1%, surplus is deionized water.
Described hyaluronic acid adopts streptococcus zooepidemicus fermentation preparation to comprise the following steps:
(1) shake-flask seed liquid is cultivated: slant strains is inoculated in the 1L conical flask that sterile medium is housed, cultivates 12-16 hour for 37 ℃, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in seed tank;
(2) strain expansion is joined: culture fluid is joined in seed tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, by aseptic requirement, in seed tank, add strain, maintain tank pressure 0.05MPa, cultivate 12-16 hour, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in fermentation tank;
(3) fermentation: half fermentation culture is joined in fermentation tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, maintain tank pressure 0.05MP, and the culture medium of seed tank is all pressed in fermentation tank, record pH value, when pH declines, add second half fermentation culture, keep indices, fermentation liquid pH fall per minute is less than at 0.01 o'clock and finishes fermentation, detect fermentation broth viscosity, fermentation liquid is pressed in settling tank;
(4) front precipitation: to the ethanol that gently adds 95% in settling tank under stirring state, to producing flakes precipitation, be controlled at 47-50 degree (folding to 20 ℃ time alcoholic strength), collecting precipitation thing;
(5) dissolve: with sodium chloride solution, precipitate is dissolved, regulate pH to 9.3-9.5, add EDTA, active carbon, perlite, stir;
(6) filter: the feed liquid after dissolving is pressed into after the large plate-and-frame filtration of 1# one time, and filtrate is adjusted pH9.3-9.5.Add again after white kieselguhr and perlite, after stirring, be pressed into the large plate-and-frame filtration of 2# second time.In two times filtrate, add kieselguhr, after stirring, feed liquid is beaten after circular clarifying by corrosion resistant plate, filters the 3rd time, and clarity is more than 99.5%, and then filtrate passes through polypropylene filter element filtering to clean room settling tank;
(7) postprecipitation: feed liquid is adjusted pH to 7.0-7.3, gently adds 95% ethanol to precipitation to produce under stirring, control ethanol number of degrees 47-50 degree.After static 20-40 minute, supernatant is squeezed into waste alcohol tank;
(8) dewater centrifugal: the dehydration of alcohol with the pH value of 75%-80% between 7.0-7.3 6-7 time, and then with 90% dehydration of alcohol 2-3 time, the alcoholic strength afterwards that dewaters is counted to more than 90%, static about 1 hour, beat supernatant, pH value is surveyed in sampling, and is controlled between 7.0-7.3.Stir centrifugal after 15-20 minute;
(9) dry: centrifugal good material is put into drying chamber, 30 ℃ of design temperatures, evacuation is dry after 3 hours, and 5 ℃ of intensifications per hour stop while establishing warm 60 ℃, detect moisture, after moisture is less than 7%, go out tank.
Culture fluid in step (1), (2), (3) comprises glucose 3-5%, yeast powder 1.5-2%, Magnesium sulfate heptahydrate 1-2%, four water manganese sulfate 0.01-0.1%, potassium dihydrogen phosphate 0.1-0.5%, sodium hydrogen phosphate 0.5-0.8%, calcium carbonate 0.1-1%, zinc chloride 0.05-0.1% by weight percentage, and surplus is water.
Step 2. concentration of sodium chloride solution is 1-5%.
Step 2. in, add EDTA, active carbon, perlite mass ratio to be, 1:10-15:20-30.
Preferably, add EDTA, active carbon, perlite mass ratio to be, 1:11:25.
Step 3. in, white kieselguhr and perlitic mass ratio are 1:1-10.
Preferably, white kieselguhr and perlitic mass ratio are 1:1.5.
In step (6), filtrate passes through 0.22 micron of polypropylene filter element filtering to clean room settling tank.
Beneficial effect of the present invention is: hyaluronic acid yield of the present invention is high, light transmittance good; protein content is low; glucuronic acid content is high, soluble, and moistening effect is good; that prepares contains hyaluronic shampoo product to good stability; clean effect is good, has no side effect, non-stimulated to skin gentleness; in the time of hair washing, have good moisturizing hair care effect, protection hair is injury-free.
The present invention adopts complex mutation technology screening to go out the strain that hyaluronic acid productivity is high, combining with fermentation technology, and hyaluronic acid product output capacity is high, can reach 7-9g/L.The hyaluronic acid that fermentation obtains is easy to separation and purification, with short production cycle.Adopt to add active carbon, perlite, white kieselguhr, EDTA and remove the impurity in hyaluronic acid in conjunction with large plate-and-frame filtration technology, additive is common to be easy to get, and operates relatively simply, and impurity content is lower.Use 75-80% ethanol to carry out 6-7 dehydration to material, 90% ethanol carries out 2-3 dehydration to material.So progressively dehydration has not only reduced the moisture in hyaluronic acid, is easy to be dried, and has washed away a small amount of impurity residual in material, and protein content is lower, and product purity is high.Use ethanol repeatedly to dewater to reach 90% when above after, can obtain Powdered amorphous precipitatedly, it is dry that product dissolubility is good afterwards, bulk density is moderate.Hyaluronic acid product quality meets the standard of cosmetic field.
the specific embodiment:
In order to understand better the present invention, with instantiation, describe technical scheme of the present invention in detail below, but the present invention is not limited thereto.
Embodiment 1
Of the present inventionly a kind ofly contain hyaluronic shampoo product technical scheme and be, a kind of streptococcus zooepidemicus (
streptococcus zooepidemicus) AWA008, the center preservation of Qi China Committee for Culture Collection of Microorganisms common micro-organisms, its deposit number is: CGMCCNo.9111, preservation date is on 04 29th, 2014.
At present this streptococcus zooepidemicus is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and postcode: 100101, its deposit number is: CGMCCNo.9111, the Latin title of strain is
streptococcus zooepidemicus, the microorganism (strain) of ginseng certificate: AWA008, preservation date is on 04 29th, 2014.
Streptococcus zooepidemicus strain characteristics of the present invention: spherical in shape or oval, be catenation, there is pod membrane, without spore, atrichia, Gram-positive.
Described streptococcus zooepidemicus is prepared hyaluronic production technology, comprises the following steps:
(1) shake-flask seed liquid is cultivated: slant strains is inoculated in the 1L conical flask that sterile medium is housed, cultivates 12-16 hour for 37 ℃, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in seed tank;
(2) strain expansion is joined: culture fluid is joined in seed tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, by aseptic requirement, in seed tank, add strain, maintain tank pressure 0.05MPa, cultivate 12-16 hour, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in fermentation tank;
(3) fermentation: half fermentation culture is joined in fermentation tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, maintain tank pressure 0.05MP, and the culture medium of seed tank is all pressed in fermentation tank, record pH value, when pH declines, add second half fermentation culture, keep indices, fermentation liquid pH fall per minute is less than at 0.01 o'clock and finishes fermentation, detect fermentation broth viscosity, fermentation liquid is pressed in settling tank;
(4) front precipitation: to the ethanol that gently adds 95% in settling tank under stirring state, to producing flakes precipitation, be controlled at 47-50 degree (folding to 20 ℃ time alcoholic strength), collecting precipitation thing;
(5) dissolve: with sodium chloride solution, precipitate is dissolved, regulate pH to 9.3-9.5, add EDTA, active carbon, perlite, stir;
(6) filter: the feed liquid after dissolving is pressed into after the large plate-and-frame filtration of 1# one time, and filtrate is adjusted pH9.3-9.5.Add again after white kieselguhr and perlite, after stirring, be pressed into the large plate-and-frame filtration of 2# second time.In two times filtrate, add kieselguhr, after stirring, feed liquid is beaten after circular clarifying by corrosion resistant plate, filters the 3rd time, and clarity is more than 99.5%, and then filtrate passes through polypropylene filter element filtering to clean room settling tank;
(7) postprecipitation: feed liquid is adjusted pH to 7.0-7.3, gently adds 95% ethanol to precipitation to produce under stirring, control ethanol number of degrees 47-50 degree.After static 20-40 minute, supernatant is squeezed into waste alcohol tank;
(8) dewater centrifugal: the dehydration of alcohol with the pH value of 75%-80% between 7.0-7.3 6-7 time, and then with 90% dehydration of alcohol 2-3 time, the alcoholic strength afterwards that dewaters is counted to more than 90%, static about 1 hour, beat supernatant, pH value is surveyed in sampling, and is controlled between 7.0-7.3.Stir centrifugal after 15-20 minute;
(9) dry: centrifugal good material is put into drying chamber, 30 ℃ of design temperatures, evacuation is dry after 3 hours, and 5 ℃ of intensifications per hour stop while establishing warm 60 ℃, detect moisture, after moisture is less than 7%, go out tank.
Culture fluid in step (1), (2), (3) comprises glucose 3%, yeast powder 1.5%, Magnesium sulfate heptahydrate 1%, four water manganese sulfates 0.05%, potassium dihydrogen phosphate 0.3%, sodium hydrogen phosphate 0.8%, calcium carbonate 0.2%, zinc chloride 0.08% by weight percentage, and surplus is water.
Step (5) concentration of sodium chloride solution is 3%.
In step (5), add EDTA, active carbon, perlite mass ratio to be, 1:11:25.
In step (6), white kieselguhr and perlitic mass ratio are 1:1.5.
In step (6), filtrate passes through 0.22 micron of polypropylene filter element filtering to clean room settling tank.
1. the present invention adopts complex mutation technology screening to go out the strain that hyaluronic acid productivity is high, combining with fermentation technology, and hyaluronic acid product productive rate reaches 9g/L.The hyaluronic acid that fermentation obtains is easy to separation and purification, with short production cycle.
2. the present invention adopts and adds active carbon, perlite, white kieselguhr, EDTA in conjunction with the impurity in large plate-and-frame filtration technology removal hyaluronic acid, additive is common to be easy to get, operate relatively simple, impurity content lower (protein≤0.1%, heavy metal≤20ppm, arsenic salt≤2ppm).
3. the present invention uses 75-80% ethanol to carry out 6-7 dehydration to material, and 90% ethanol carries out 2-3 dehydration to material.So progressively dehydration has not only reduced the moisture in hyaluronic acid, is easy to be dried, and has washed away a small amount of impurity residual in material, and protein content is lower, and product purity is high, can reach more than 99.0%.
The present invention use ethanol repeatedly to dewater to reach 90% when above after, can obtain Powdered amorphous precipitated, dry after product dissolubility good, dissolution velocity is fast, degraded is slow, bulk density moderate (bulk density 0.4～0.6g/ml).
5. production technology of the present invention is simple, and supplementary material is common to be easy to get, and produces and is easy to control, and impurity content is lower, is applicable to large-scale industrialization and produces, and yield is high, and greatly reduces production cost.
Embodiment 2
A kind ofly contain hyaluronic shampoo product, comprise following composition by weight: trisodium citrate 0.5%, lauric acid 15%, hyaluronic acid 2%, methyl butex 0.08%, dipropylene glycol 5%, hydrolysis aleuronat 1.2%, potassium sorbate 0.2%, disodiumedetate 0.2%, essence 0.1%, surplus is deionized water.
Hyaluronic acid yield of the present invention is high, light transmittance good; protein content is low; glucuronic acid content is high, soluble, and moistening effect is good; that prepares contains hyaluronic shampoo product to good stability; clean effect is good, has no side effect, non-stimulated to skin gentleness; in the time of hair washing, have good moisturizing hair care effect, protection hair is injury-free.
Embodiment 3
Described a kind of hyaluronic shampoo product that contains, comprise following composition by weight: trisodium citrate 0.6%, lauric acid 16%, hyaluronic acid 3%, methyl butex 0.05%, dipropylene glycol 8%, hydrolysis aleuronat 1%, potassium sorbate 0.3%, disodiumedetate 0.3%, essence 0.2%, surplus is deionized water.
Hyaluronic acid yield of the present invention is high, light transmittance good; protein content is low; glucuronic acid content is high, soluble, and moistening effect is good; that prepares contains hyaluronic shampoo product to good stability; clean effect is good, has no side effect, non-stimulated to skin gentleness; in the time of hair washing, have good moisturizing hair care effect, protection hair is injury-free.
Claims (8)
1. contain a hyaluronic shampoo product, it is characterized in that, this shampoo product contains hyaluronic acid, described hyaluronic acid employing streptococcus zooepidemicus (
streptococcus zooepidemicus) the AWA008 preparation of fermenting, streptococcus zooepidemicus (
streptococcus zooepidemicus) AWA008 deposit number is: CGMCCNo.9111, preservation date is on 04 29th, 2014.
2. a kind of hyaluronic shampoo product that contains according to claim 1, it is characterized in that, comprise following composition by weight: trisodium citrate 0.2-0.8%, lauric acid 10-20%, hyaluronic acid 0.5-5%, methyl butex 0.05-0.2%, dipropylene glycol 1-10%, hydrolysis aleuronat 1-2%, potassium sorbate 0.1-0.5%, disodiumedetate 0.1-0.5%, essence 0.1-0.3%, surplus is deionized water.
3. a kind of hyaluronic shampoo product that contains according to claim 2, it is characterized in that, comprise following composition by weight: trisodium citrate 0.3-0.6%, lauric acid 12-18%, hyaluronic acid 1-3%, methyl butex 0.05-0.1%, dipropylene glycol 2-8%, hydrolysis aleuronat 1-1.5%, potassium sorbate 0.1-0.3%, disodiumedetate 0.1-0.3%, essence 0.1-0.2%, surplus is deionized water.
4. a kind of hyaluronic shampoo product that contains according to claim 2, it is characterized in that, comprise following composition by weight: trisodium citrate 0.5%, lauric acid 15%, hyaluronic acid 2%, methyl butex 0.08%, dipropylene glycol 5%, hydrolysis aleuronat 1.2%, potassium sorbate 0.2%, disodiumedetate 0.2%, essence 0.1%, surplus is deionized water.
5. a kind of hyaluronic shampoo product that contains according to claim 1, is characterized in that, described hyaluronic acid adopts streptococcus zooepidemicus fermentation preparation to comprise the following steps:
(1) shake-flask seed liquid is cultivated: slant strains is inoculated in the 1L conical flask that sterile medium is housed, cultivates 12-16 hour for 37 ℃, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in seed tank;
(2) strain expansion is joined: culture fluid is joined in seed tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, by aseptic requirement, in seed tank, add strain, maintain tank pressure 0.05MPa, cultivate 12-16 hour, it is good that microscopy is observed thalli growth, without living contaminants, can be inoculated in fermentation tank;
(3) fermentation: half fermentation culture is joined in fermentation tank, carry out after steam sterilization, 36.3 ℃ of design temperatures, pH6.86, maintain tank pressure 0.05MP, and the culture medium of seed tank is all pressed in fermentation tank, record pH value, when pH declines, add second half fermentation culture, keep indices, fermentation liquid pH fall per minute is less than at 0.01 o'clock and finishes fermentation, detect fermentation broth viscosity, fermentation liquid is pressed in settling tank;
(4) front precipitation: to the ethanol that gently adds 95% in settling tank under stirring state, to producing flakes precipitation, be controlled at 47-50 degree, collecting precipitation thing;
(5) dissolve: with sodium chloride solution, precipitate is dissolved, regulate pH to 9.3-9.5, add EDTA, active carbon, perlite, stir;
(6) filter: the feed liquid after dissolving is pressed into after the large plate-and-frame filtration of 1# one time, and filtrate is adjusted pH9.3-9.5; Add again after white kieselguhr and perlite, after stirring, be pressed into the large plate-and-frame filtration of 2# second time; In two times filtrate, add kieselguhr, after stirring, feed liquid is beaten after circular clarifying by corrosion resistant plate, filters the 3rd time, and clarity is more than 99.5%, and then filtrate passes through polypropylene filter element filtering to clean room settling tank;
(7) postprecipitation: feed liquid is adjusted pH to 7.0-7.3, gently adds 95% ethanol to precipitation to produce under stirring, control ethanol number of degrees 47-50 degree; After static 20-40 minute, supernatant is squeezed into waste alcohol tank;
(8) dewater centrifugal: the dehydration of alcohol with the pH value of 75%-80% between 7.0-7.3 6-7 time, and then with 90% dehydration of alcohol 2-3 time, the alcoholic strength afterwards that dewaters is counted to more than 90%, static about 1 hour, beat supernatant, pH value is surveyed in sampling, and is controlled between 7.0-7.3; Stir centrifugal after 15-20 minute;
(9) dry: centrifugal good material is put into drying chamber, 30 ℃ of design temperatures, evacuation is dry after 3 hours, and 5 ℃ of intensifications per hour stop while establishing warm 60 ℃, detect moisture, after moisture is less than 7%, go out tank.
6. a kind of hyaluronic shampoo product that contains according to claim 5, it is characterized in that, culture fluid in step (1), (2), (3) comprises glucose 3-5%, yeast powder 1.5-2%, Magnesium sulfate heptahydrate 1-2%, four water manganese sulfate 0.01-0.1%, potassium dihydrogen phosphate 0.1-0.5%, sodium hydrogen phosphate 0.5-0.8%, calcium carbonate 0.1-1%, zinc chloride 0.05-0.1% by weight percentage, and surplus is water.
7. a kind of hyaluronic shampoo product that contains according to claim 5, is characterized in that, in step (5), adds EDTA, active carbon, perlite mass ratio to be, 1:10-15:20-30.
8. a kind of hyaluronic shampoo product that contains according to claim 5, is characterized in that, in step (6), white kieselguhr and perlitic mass ratio are 1:1-10.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104069018A (en) * | 2014-06-11 | 2014-10-01 | 滨州安华生物工程有限公司 | Liquid shampoo |
| CN108410926A (en) * | 2017-12-03 | 2018-08-17 | 新疆阜丰生物科技有限公司 | A method of preparing extraction high molecular weight hyaluronic acid |
| CN114805636A (en) * | 2022-05-26 | 2022-07-29 | 宁夏华吉生物有限公司 | Method for preparing sodium hyaluronate |
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Cited By (5)
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Application publication date: 20141001 |