CN104067923B - The breeding method of a kind of high protein, homovitamin C content radish seed bud dish - Google Patents
The breeding method of a kind of high protein, homovitamin C content radish seed bud dish Download PDFInfo
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Abstract
The invention discloses the breeding method of a kind of high protein, homovitamin C content radish seed bud dish, selection vermiculite is culture medium, gets fresh full radish seed, with 50-55 DEG C of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water washes 2 times; By seed uniform broadcasting in matrix, cover vermiculite 1.5-2.0cm; Sowing, is mixed with the 0.5-2g/L aqueous solution, pours milk soln into culture matrix by milk, controlled humidity, at 50-60%, is cultivated 5-7 days in 20-30 DEG C, gathered in the crops.The present invention is suitable for intensive cultivation high protein, homovitamin C content radish seed bud dish; Technological feasibility is strong, is not affected by the external environment, without chemical fertilizer, hormone and agricultural chemicals in the production process of shoot vegetable; Place used and nutrient solution cost low, invest little, instant effect, growth cycle are short, output is high, added value of product is high, can be used for the batch production of bud dish, intensive manufacture.
Description
Technical field
The present invention relates to a kind of breeding method of radish seed bud dish, particularly relate to the breeding method of a kind of high protein, homovitamin C content radish seed bud dish, belong to agricultural production, raw-food material production field.
Background technology
Bud class is traditional high quality food that compatriots like.The food that bud-genus food refers generally to cereal, beans produces through germination, commonly moyashi, mung bean sprouts.The nutritive value of bud dish is higher, because its vegetative period is short, does not need fertilising, just can harvest well without the need to laxative, is non-harmful vegetables.Radish seed, another name SEMEN RAPHANI, trailing plants white chessman, dish chieftain, Latin literary fame SemenRaphani. is the mature seed of crucifer radish, is used for the treatment of retention of food and drink, abdominal distention, constipation, stagnant rushes down dysentery, phlegm stops up and breath with cough.Containing abundant nutriment in radish seed bud dish, vitamin content is equivalent to 10 times of Chinese cabbage.Radish seed bud dish is slightly with pungent taste, edible very easy, therefore can go deep into rapidly general family, and the most important thing is that it can water planting, plantation is convenient.In recent years, people like adding bud dish eating in barbecue, western-style food or salad, not only good to eat but also nutrition.As at taste noise juice or add ternip seedling with meat and nurse one's health in the lump, not only can promote gastrointestinal disturbances, and can eliminate greasy.
Summary of the invention
The object of the present invention is to provide the breeding method of a kind of high protein, homovitamin C content radish seed bud dish, radish seed germination condition is screened, to increase radish seed germination rate, improve albumen, the vitamin content of radish seed bud dish, to provide high-quality radish bud dish.
For achieving the above object, the present invention is by the following technical solutions:
A breeding method for high protein, homovitamin C content radish seed bud dish, the method comprises the following steps:
(1) select culture medium: take vermiculite as culture medium, load in nutrient discs, the thickness of vermiculite is 5-7cm;
(2) choose seeds: select fresh full radish seed, used 50-55 DEG C of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water washes 2 times;
(3) sow: by seed uniform broadcasting in matrix, cover vermiculite 1.5-2.0cm;
(4) add culture fluid: milk is mixed with the 0.5-2g/L aqueous solution, pours milk soln into culture matrix, humidity is agglomerating to hold with hand, and namely stretching out one's hand falls apart is advisable;
(5) manage: cultivate 5-7 days in 20-30 DEG C, gather in the crops.
The present invention selects vermiculite to be culture matrix, and vermiculite is a kind of natural, nontoxic mineral matter, dilatable mineral under high temperature action.Because vermiculite has the ability of ion exchange, it has great effect to the nutrition of soil.Vermiculite can promote the stable growth of the growth of root system of plant and seedling effectively.The necessary moisture of plant growth and nutrition are provided for a long time, and the stable of plant root temperature can be kept.Vermiculite can make crop just can obtain sufficient moisture and mineral matter from early growth period, promotes that plant comparatively fast grows, increase yield.
Preferably, described nutrient discs is placed on semi-enclosed layer stereo support, and so-called semi-enclosed layer stereo support is three side sealing and closes, the layer stereo support that top, bottom and operating surface are exposed.This semi-enclosed layer stereo support, both for putting multilayer seedling dish, carries out vertical cultivation, both can improve and utilize space, cost-saving, contributes to again keeping certain humidity.
Preferably, the humid control of the middle culture matrix of described step (4) is at 50-60%.
Preferably, the concentration of the aqueous solution of described milk is 1g/L; In described step (5), cultivation temperature is 20 DEG C, and incubation time is 7 days.
The present invention breaches the traditional mode of production pattern of radish seed (SEMEN RAPHANI) bud dish, has initiated and has utilized simple machine to carry out novel standardization intensive production new model under severe conditions.Beneficial effect of the present invention is:
1, the present invention is suitable for intensive cultivation high protein, homovitamin C content radish seed bud dish.
2, present invention process feasibility is strong, is not affected by the external environment, without chemical fertilizer, hormone and agricultural chemicals in the production process of shoot vegetable.
3, the present invention place used and nutrient solution cost low, invest little, instant effect, growth cycle are short, output is high, added value of product is high, can be used for the batch production of bud dish, intensive manufacture.
4, the Product Safety adopting the present invention to cultivate is high, and environmentally safe, has really met people to demand that is natural, nuisanceless, high-quality, to the yearning of pure life, is the green organic products meeting current era meaning.
Embodiment
Below by way of specific embodiment, the present invention will be further described.
The mensuration of protein content and the mensuration of Vitamin C content adopt following methods respectively in the examples below.
1, protein content is measured with Coomassie Brilliant Blue
(1) preparation of standard liquid: accurately take bovine serum albumin(BSA) 100.00mg, distilled water dissolves, and be settled to 1000mL, this is 0.1g/L protein standards storing solution, Refrigerator store.
(2) preparation of Coomassie brilliant G-250 solution: precision takes Coomassie brilliant G-250 100.00mg, adds 95% ethanol 50mL (blueness), then adds 85% (W/V) H
3pO
4100mL (blood red) is finally settled to 1000mL (brown) with distilled water.
(3) preparation of need testing solution: take drying sample 0.5g, adds a small amount of quartz sand distilled water and grinds to form homogenate and be settled to 100mL, and leave standstill 10-30min (3-5min, shake once) and filter, it is for subsequent use that filtrate is settled to 100mL.
(4) Specification Curve of Increasing: standard liquid 1mL, after adding Coomassie brilliant G-250 protein reagent, shakes up, after placing 2min, and colorimetric estimation under 595nm wavelength, record A595.With protein content (X) for abscissa with absorbance (A) for ordinate carries out linear regression analysis, equation of linear regression is: A=1.565X-0.005, coefficient R
2=0.999.
(5) sample size measures: get 2, test tube, 1 the preparation 1mL liquid to be measured adding need testing solution, and 1 adds 1mL distilled water (doing blank), add 5.0mL Coomassie brilliant G-250 reagent again, shake up, after placing 5min, colorimetric under 595nm wavelength, record A595.By Specification Curve of Increasing operation, measure absorbance, record data, calculate protein content.
2, the mensuration of Vitamin C content
The drafting pipette of calibration curve draws 1g/L vitamin C titer 0.00mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL and 1.00mL respectively to 6 clean 50mL test tubes, be settled to scale, with 0.00mL vitamin C mark liquid for blank makes reference, under 267nm wavelength, use 1cm quartz colorimetric utensil, measure absorbance.With the mass concentration of vitamin C serial solution for abscissa, with the absorbance of correspondence for ordinate, draw out calibration curve.Data are carried out recurrence process, result shows, ascorbic mass concentration is linear within the scope of 0 ~ 20mg/L, and equation of linear regression is: A=0.06163X-0.02934, coefficient R
2=0.998.
3, ascorbic extraction and mensuration in radish seed sample
Cleaned by bud dish, drain, chopping, mixing, accurately take 10g respectively in grinding alms bowl, add the hydrochloric acid of 10mL1%, homogenate moves in 50mL volumetric flask, with distilled water diluting to scale.Accurate measuring 1mL extract respectively, wherein 1mL puts into the 50mL test tube filling 2mL10% hydrochloric acid, measures it in 267nm place absorbance.Vitamin C content is calculated by calibration curve.
Embodiment
1, seed sprouting pre-treatment
The germination of seed and pre-treatment, select that color and luster is fresh, full seed, free from insect pests, without going rotten, without broken beans, high quality seed that germination vigor is strong, with clear water rinsing 2-3 time, seed 50-55 DEG C of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water washes 2 times, stand-by.
2, culture medium is selected
Select vermiculite, fine sand, light soil 3 kinds of seedling mediums to be culture medium, load nutrient discs, stromal thickness 5-7cm, nutrient discs is put to semi-enclosed layer stereo support.Pour water into culture matrix, the humidity controlling culture matrix is within 60%, 5 days, observe its germination vigor also to record the seed sprouting number often organized, and measure average height, weightless dry weight, calculate germination rate, upgrowth situation is in table 1.
Table 1 different substrates germinates on radish seed and affects (100 seeds)
| Culture matrix | Fine sand | Vermiculite | Light soil |
| Sprout number | 93 | 98 | 92 |
| Bud ratio | 93％ | 98％ | 92％ |
| Bud dish average height | 7.5cm | 9.2cm | 8.2cm |
| Weight in wet base | 5.235g | 6.668g | 5.870g |
| Dry weight | 0.278g | 0.323g | 0.293g |
As can be seen from Table 1, be that matrix radish seed germination rate and dry weight in wet base are all higher with vermiculite, selection vermiculite is culture matrix.
3, temperature, milk concn dialogue radish seed growth effect
Under 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C constant temperatures, add nutrient solution (milk or water) respectively, add variable concentrations milk soln, carry out germination test.Wherein vermiculite test is by seed uniform broadcasting in matrix, covers vermiculite 1.5cm.Milk concn is the 1.0g-10/L aqueous solution, pour milk soln into culture matrix, the humidity controlling culture matrix is within the 60%, 5th day, observe its germination vigor also to record the seed sprouting number often organized, measure average height, weightless dry weight, calculate germination rate, upgrowth situation is in Table 2-5.
Table 2 radish seed is at 15 DEG C of germination (100 seeds)
| Culture fluid | Add water | 0.5g/L milk | 1g/L milk | 5g/L milk | 10g/L milk |
| Sprout number | 93 | 92 | 97 | 95 | 91 |
| Bud ratio | 93％ | 92％ | 97％ | 95％ | 91％ |
| Bud dish average height | 8.3cm | 8.3cm | 8.8cm | 8.7cm | 8.7cm |
| Weight in wet base | 5.962 | 6.077g | 6.522g | 6.136g | 5.871g |
| Dry weight | 0.302 | 0.301g | 0.316g | 0.311g | 0.305g |
Drawn by table 2: according to bud ratio, bud dish average height, and moisture content compares and draws, at 15 DEG C of temperature, is culture matrix with vermiculite, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 3 radish seed is at 20 DEG C of germination (100 seeds)
| Add culture fluid | Add water | 0.5g/L milk | 1g/L milk | 5g/L milk | 10g/L milk |
| Sprout number | 90 | 94 | 92 | 90 | 88 |
| Bud ratio | 90％ | 94％ | 92％ | 90％ | 88％ |
| Bud dish average height | 8.3cm | 8.4cm | 9.2cm | 8.8cm | 8.1cm |
| Weight in wet base | 5.422g | 6.138g | 6.436g | 6.024g | 5.631g |
| Dry weight | 0.306g | 0.302g | 0.318g | 0.336g | 0.311g |
Drawn by table 3: according to bud ratio, bud dish average height, and moisture content compares and draws, at 20 DEG C of temperature, is culture matrix with vermiculite, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 4 radish seed is at 25 DEG C of germination (100 seeds)
| Add culture fluid | Add water | 0.5g/L milk | 1g/L milk | 5g/L milk | 10g/L milk |
| Sprout number | 91 | 92 | 93 | 90 | 88 |
| Bud ratio | 91％ | 92％ | 93％ | 90％ | 88％ |
| Bud dish average height | 8.5cm | 8.8cm | 9.1cm | 8.6cm | 8.4cm |
| Weight in wet base | 5.386g | 6.358g | 6.524g | 6.109g | 5.620g |
| Dry weight | 0.312g | 0.317g | 0.338g | 0.341g | 0.302g |
Drawn by table 4: according to bud ratio, bud dish average height, and moisture content compares and draws, at 25 DEG C of temperature, is culture matrix with vermiculite, adding 1g/L milk is that the bud dish growing state of culture fluid is best.
Table 5 radish seed is at 30 DEG C of germination (100 seeds)
| Culture fluid | Add water | 0.5g/L milk | 1g/L milk | 5g/L milk | 10g/L milk |
| Sprout number | 93 | 98 | 97 | 95 | 91 |
| Bud ratio | 93％ | 98％ | 97％ | 95％ | 91％ |
| Bud dish average height | 9.2cm | 9.9cm | 9.7cm | 9.0cm | 8.7cm |
| Weight in wet base | 5.962g | 6.321g | 6.522g | 6.136g | 5.871g |
| Dry weight | 0.322g | 0.329g | 0.346g | 0.331g | 0.305g |
Drawn by table 5: according to bud ratio, bud dish average height, and moisture content compares and draws, at 30 DEG C of temperature, is culture matrix with vermiculite, adding 0.5g/L milk is that the bud dish growing state of culture fluid is best.
Sum up: draw according to table 1-5, radish seed bud dish, at 20-30 DEG C, take vermiculite as culture matrix, and concentration is the milk of 1g/L is that culture fluid bud dish growing state is good.
4, incubation time dialogue radish seed growth effect
Radish seed bud dish, at 25 DEG C, take vermiculite as culture matrix, and concentration is the milk of 1g/L is culture fluid, observes the impact of different incubation time on bud seedling output.Result is as shown in table 6.
The impact (100 seeds) that table 6 incubation time grows radish seed
| Cultivated days (d) | 2 | 4 | 6 | 8 |
| Sprout number | 97 | 97 | 97 | 97 |
| Bud ratio | 97％ | 97％ | 97％ | 97％ |
| Bud dish average height | 2.3cm | 6.1cm | 9.2cm | 9.7cm |
| Weight in wet base | 3.242g | 5.380g | 7.098g | 7.256g |
| Dry weight | 0.208g | 0.315g | 0.360g | 0.371g |
According to bud ratio, bud dish average height and dry weight in wet base, increasing output with incubation time increases, and considers manpower and material cost, should cultivate 5-7 days.
5, temperature, incubation time are on the impact of radish seed protein content
Take vermiculite as culture matrix, concentration is the milk of 1g/L is culture fluid.Different temperatures, the change of cultivation different time radish seed protein content.Result is as shown in table 7.
Table 7 temperature, incubation time are on the impact (mg/g) of radish seed protein content
Drawn by table 7: high at the content of the protein of 20 DEG C of bud dishes, cultivate 1-2 days protein contents high, consider bud dish output (dry, weight in wet base), should 5-7 days be cultivated.
6, milk soln and water are on the impact of protein content
Take vermiculite as culture matrix, 20 DEG C of cultivations, concentration to be the milk of 1g/L be culture fluid and water are on the impact of protein content.Result is as shown in table 8.
Table 8 milk soln and water are on the impact (mg/g) of protein content
Drawn by table 8: under equal conditions, milk soln can significantly improve bud dish protein content.
7, radish seed Vitamin C content (mg/100g) measures orthogonal experiment
Take vermiculite as culture matrix, temperature, milk concn, cultivation number of days are influence factor, design 3 levels, observe the change of Vitamin C content.Result is as shown in table 9.
Table 9 radish seed Vitamin C content (mg/100g) measures orthogonal experiment
| Horizontal factor | Temperature (DEG C) | Milk concn (g/L) | Number of days (D) |
| 1 | 19 | 0 | 4 |
| 2 | 20 | 1 | 6 |
| 3 | 21 | 1.1 | 8 |
Select L9 (34) orthogonal table, the 4th row can be arranged without factor, as sky row, do not arrange factor.According to orthogonal table requirement, carry out nine experiments, using Vitamin C content height as inspection target, the results are shown in Table 10.
Table 10 radish seed Vitamin C content (mg/100g)
| Column | 1 | 2 | 3 | 4 | |
| Factor | Temperature | Milk concn | Number of days | Empty row | Experimental result |
| Experiment 1 | 1 | 1 | 1 | 1 | 48.96 |
| Experiment 2 | 1 | 2 | 2 | 2 | 52.32 |
| Experiment 3 | 1 | 3 | 3 | 3 | 52.38 |
| Experiment 4 | 2 | 1 | 2 | 3 | 60.14 |
| Experiment 5 | 2 | 2 | 3 | 1 | 56.44 |
| Experiment 6 | 2 | 3 | 1 | 2 | 47.21 |
| Experiment 7 | 3 | 1 | 3 | 2 | 46.79 |
| Experiment 8 | 3 | 2 | 1 | 3 | 51.46 |
| Experiment 9 | 3 | 3 | 2 | 1 | 50.09 |
| Average 1 | 52.200 | 51.963 | 49.210 | 51.830 | |
| Average 2 | 54.597 | 54.407 | 55.183 | 49.773 | |
| Average 3 | 49.447 | 49.873 | 51.850 | 54.640 | |
| Extreme difference | 5.150 | 14.534 | 5.973 | 4.867 |
The extreme difference value of more each factor can be seen, the growth temperature that radish seed Vitamin C content is the highest is 20 DEG C, and the concentration of culture fluid milk is 1g/L.Best growth number of days is 7 days.
Claims (5)
1. a breeding method for high protein, homovitamin C content radish seed bud dish, it is characterized in that, the method comprises the following steps:
(1) select culture medium: take vermiculite as culture medium, load in nutrient discs, the thickness of vermiculite is 5-7cm;
(2) choose seeds: select fresh full radish seed, used 50-55 DEG C of emerge in worm water 15min, pull out, then soak 15min with 1% liquor potassic permanganate, pull out, clear water washes 2 times;
(3) sow: by seed uniform broadcasting in matrix, cover vermiculite 1.5-2.0cm;
(4) add culture fluid: milk is mixed with the 0.5-2g/L aqueous solution, pour the aqueous solution of milk into culture matrix, humid control is agglomerating for holding with hand, stretches out one's hand and namely falls apart;
(5) manage: cultivate 5-7 days in 20-30 DEG C, gather in the crops.
2. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, it is characterized in that, described nutrient discs is placed on semi-enclosed layer stereo support.
3. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, in described step (4), the humid control of culture matrix is at 50-60%.
4. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, it is characterized in that, the concentration of the aqueous solution of described milk is 1g/L.
5. the breeding method of high protein according to claim 1, homovitamin C content radish seed bud dish, is characterized in that, in described step (5), cultivation temperature is 20 DEG C, and incubation time is 7 days.
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| CN111937730A (en) * | 2019-05-14 | 2020-11-17 | 临洮县兴望牡丹产业有限责任公司 | Purple-spot peony bud cultivation method and cultivation system thereof |
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