CN104059926A - Related genes for synthesis of eggplant anthocyanin and coding protein of related genes - Google Patents
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Abstract
The invention relates to related genes for synthesis of eggplant anthocyanin and coding protein thereof, belonging to the biotechnology field. The gene is SmTTG1gene, or SmGL3 gene, or SmTT8 gene, the coded amino acid sequence of the SmTTG1 gene is shown in SEQ ID NO.2, the coded amino acid sequence of the SmGL3 gene is shown in SEQ ID NO.4, and the coded amino acid sequence of the SmTT8 gene is shown in SEQ ID NO.6. By using the SmTTG1gene, or SmGL3 gene or SmTT8 gene of the eggplant, downstream genes regulated and controlled by the genes and closely related to synthesis of the anthocyanin and other transcription factor genes interactive with the genes can be screened out, so that the regulatory mechanism for synthesis path of the eggplant anthocyanin is explored. The gene sequence of the eggplant can be changed by locus mutation of the SmTTG1gene, or SmGL3 gene or SmTT8 gene of the eggplant, so as to change the characters of the eggplant fruit to improve the ornamental value and yield and higher economic value.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of eggplant anthocyanidin synthesis related gene and proteins encoded thereof.
Background technology
Anthocyanidin is the important water-soluble natural plant pigments of a class, belongs to flavonoid material, be extensively present in the vacuole of plant epidermis cell, present orange, red to blue.Anthocyanidin is a lot, wherein common with these three kinds of cyanidin, pelargonidin and delphinidins according to its basic structure classification.Anthocyanidin is the most potent free-radical scavengers found up to now, has oxidation-resisting and caducity, preventing cardiovascular disease, antitumor, anti-mutation, adjusting immunocompetence function; In addition anthocyanidin or a kind of safe, nontoxic natural food colour.Anthocyanidin has a wide range of applications at aspects such as gardening, food, healthcare products, and human health is had to huge potential value, so anthocyanidin is the hot fields of research always.
Anthocyanidin biosynthesizing in plant is by the co-controlling of two genoids, and a class is structure gene, the key enzyme in coding Kuromanine biosynthetic pathway; Another kind of is regulatory gene, expression intensity and the pattern of regulation and control Kuromanine biosynthesis gene, and control Kuromanine in the variation of spatial and temporal expression.At present separated and identified the synthetic transcription factor of three class anthocyanidin: the bHLH albumen of R2R3-MYB albumen, MYC family and WD40 albumen.These transcription factors are combined by corresponding cis-acting elements in structure gene promotor, thereby regulate the expression of gene in anthocyanin biosynthetic pathway.In this process, be generally to form a protein complexes (being called for short MBW protein complexes) by myb transcription factor, bHLH transcription factor and WD40 transcription factor, direct regulation and control structure gene is transcribed.
At present, from the plants such as Arabidopis thaliana, petunia, purple perilla, corn, grape, Common Snapdragon, apple separating clone a large amount of structure gene and regulatory genes relevant to anthocyanidin biosynthetic metabolism, still in this respect to the fresh rare report of the research of eggplant.In this research separating clone the synthetic relevant WD40 transcription factor gene SmTTG1 of eggplant anthocyanidin, bHLH transcription factor gene SmGL3 and SmTT8, them have been studied at the expression specificity of eggplant different tissues, and utilize yeast two-hybrid system, detect the albumen of these translations and the albumen of the myb transcription factor gene SmMYB formation situation of doing mutually between any two, for further probing into eggplant anthocyanidin biosynthetic controlling mechanism, provide basis.By prior art documents, not yet find the technical theme identical or similar with the present invention.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of eggplant anthocyanidin synthesis related gene and proteins encoded thereof are provided.The present invention is directed to the present situation to eggplant Research foundation weakness at present, clone the synthetic associated transcription factor SmTTG1 of eggplant anthocyanidin, SmGL3, the gene order of SmTT8, and then can, by operations such as the regulation and control of this genetic expression and interference, improve the quality of eggplant.
The present invention realizes by following technical scheme, the present invention relates to a kind of eggplant anthocyanidin synthesis related gene, and described gene is SmTTG1 gene or SmGL3 gene or SmTT8 gene;
The aminoacid sequence of described SmTTG1 genes encoding is as shown in SEQ ID NO.2;
The aminoacid sequence of described SmGL3 genes encoding is as shown in SEQ ID NO.4;
The aminoacid sequence of described SmTT8 genes encoding is as shown in SEQ ID NO.6.
Preferably, the sequence of described SmTTG1 gene is as shown in SEQ ID NO.1.
Preferably, the sequence of described SmGL3 gene is as shown in SEQ ID NO.3.
Preferably, the sequence of described SmTT8 gene is as shown in SEQ ID NO.5.
The present invention, according to Genebank database, uses information biology means, by sequence alignment, designs degenerated primer, and the method with molecular cloning, clones SmTTG1 in purple eggplant, SmGL3, SmTT8 gene; Through sequential analysis, determine it is the genes involved of eggplant; Tissue expression analysis is found, SmTTG1, each histofluorescence half-quantitative detection value of SmGL3 and SmTT8 gene, result shows, three genes at root, stem, also, all have expression in flower, pericarp, pulp, but in different tissues expression amount difference.SmTTG1 gene expression level in blade is the highest, is significantly higher than other each tissues; In its hetero-organization, expression amount is suitable, there was no significant difference.The highest other each tissues that is significantly higher than of SmGL3 gene expression amount in stem, are secondly flower and pulp, and in root and leaf, expression amount is very low.The expression amount of SmTT8 in pericarp is the highest, also has remarkable expression in stem, and in root, leaf, flower, pulp, expression amount is low, and there was no significant difference.
The present invention has following beneficial effect: SmTTG1 of the present invention, and SmGL3, SmTT8 is that transcription factor gene is the synthetic key controlling gene of eggplant anthocyanidin, this gene is by regulate the expression of downstream gene in conjunction with cis element, thus regulation and control anthocyanidin is synthetic.Utilize eggplant SmTTG1 of the present invention, SmGL3, SmTT8 gene, can filter out be subject to its regulation and control with the synthetic closely-related downstream gene of anthocyanidin, and other transcription factor genes interactional with it, thus probe into eggplant anthocyanidin synthesis path regulatory mechanism.Utilize eggplant SmTTG1, SmGL3, SmTT8 gene also can change the gene order in eggplant, thereby eggplant fruit properties is changed by gene is carried out to site mutation, reaches and improves its ornamental value and output, realizes higher economic worth.
Accompanying drawing explanation
By reading the detailed description of non-limiting example being done with reference to the following drawings, it is more obvious that other features, objects and advantages of the present invention will become:
Fig. 1 is SmTTG1 of the present invention, SmGL3, the cDNA agarose gel electrophoresis figure of SmTT8 gene;
Fig. 2 is gene SmTTG1 tissue expression analysis chart of the present invention;
Fig. 3 is gene SmGL3 tissue expression analysis chart of the present invention;
Fig. 4 is gene SmTT8 tissue expression analysis chart of the present invention;
Fig. 5 is SmTTG1 of the present invention, SmGL3, the yeast two-hybrid proof diagram between SmTT8 and SmMYB albumen.
Embodiment
Below in conjunction with specific embodiment and accompanying drawing, further set forth the specific embodiment of the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.The experimental technique of unreceipted actual conditions in lower routine embodiment, conventionally according to normal condition, the molecular cloning cloning experimentation handbook of writing such as (1989) such as Sambrook (New York:Cold Spring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.
The test kits such as the RNA extraction the present invention relates to, PCR purifying, plasmid recovery obtain by disclosing commercially available commercial channel; enough buy in biological (Shanghai) limited-liability company of raw work; intestinal bacteria (Escherichia coli) bacterial strain DH-5 α is preserved by this laboratory; Taq archaeal dna polymerase, restriction endonuclease, AMV ThermoScript II, gel recovery test kit and synthetic primer are purchased from Sheng Gong biotech firm
max DNA high-fidelity enzyme, pMD19-T carrier, RACE amplification kit are purchased from the precious biotech firm (TaKaRa) in Dalian.
Fig. 1 is SmTTG1 of the present invention, SmGL3, the cDNA agarose gel electrophoresis figure of SmTT8 gene; SmTTG1 gene fragment (A), SmGL3 gene fragment, (B) SmTT8 gene fragment (C) and SmMYB gene fragment (D); Fig. 2 is gene SmTTG1 tissue expression analysis chart of the present invention; Fig. 3 is gene SmGL3 tissue expression analysis chart of the present invention; Fig. 4 is gene SmTT8 tissue expression analysis chart of the present invention; Fig. 5 is SmTTG1 of the present invention, SmGL3, the yeast two-hybrid proof diagram between SmTT8 and SmMYB albumen.
embodiment 1, eggplant anthocyanidin synthetic relevant SmTTG1, SmGL3, the clone of SmTT8 and tissue expression analysis
The present embodiment relates to the synthetic relevant SmTTG1 of eggplant anthocyanidin, SmGL3, and the clone of SmTT8 and tissue expression analysis, comprise the steps:
Step 1, the synthetic relevant SmTTG1 of eggplant anthocyanidin, SmGL3, the clone of SmTT8 gene
1, eggplant experiment material is drawn materials
This tests the eggplant fine germplasm resources YZ14 (Shao Wenting that vegetable material Wei Ben seminar used preserves, Liu Yang, clone and the functional study of the strong 2013. eggplant anthocyanidin synthesis related gene SmMYB of Han Hong. gardening journal 40 (3): 467 – 478), YZ14 phenotype is stingless, purple stem, pale reddish brown, purple fruit.Experiment material is cultivated in the artificial plastic greenhouse of Pujiang town, Minhang District, Shanghai space-breeding land.Grow seedlings under field conditions (factors), growth is also solid.Get root, purple stem, purple blade, purple petal, the purple fruit of purple eggplant.
2, TRIzol method is extracted the total RNA in commodity ripening stage purple pericarp;
(1) take the commodity ripening stage purple pericarp of 0.5g purple long eggplant, with after liquid nitrogen flash freezer, grind to form rapidly fine powder, be sub-packed in the eppendorf centrifuge tube of 1.5ml;
(2) add 1ml TRIzol, firmly shake and make to mix, 25 ℃, place 5min;
(3) every pipe adds 0.2ml chloroform, uses forced oscillation 15sec, and room temperature is placed 2min-3min, 4 ℃, 12,000g, centrifugal 15min;
(4) supernatant liquor 600ul is sucked in the new eppendorf centrifuge tube of 1.5ml, add and supernatant equal-volume Virahol, put upside down and mix, in-20 ℃ of refrigerators, place 30min;
(5) 4 ℃, 12,000g, centrifugal 15min, abandons supernatant, collect RNA precipitation, add 1ml75% (V/V) washing with alcohol precipitation 1-2 time, washing is specially: add 1ml75% (V/V) ethanol, 4 ℃ centrifugal, 12000g, 10min-15min, abandons supernatant, collects RNA precipitation;
(6) dry 15min-20min at 25 ℃ of precipitations, is dissolved in the RNase-free water of 30 μ l-50 μ l;
(7) with denaturing formaldehyde gel electrophoresis, identify total RNA quality, then on spectrophotometer, measure rna content.
3, SmTTG1, SmGL3, the clone of SmTT8 gene cDNA total length and the amplification of SmMYB gene
According to gene TTG1 synthetic relevant to anthocyanidin in Arabidopis thaliana, GL3, the sequence information of TT8, in NCBI, carry out after BLAST comparison, select wherein the homologous protein sequence of the plant near with eggplant sibship, and obtain its corresponding encoding sequence (CDS), conserved regions design for two ends can amplify the degenerated primer (table 7) of total length and take eggplant cDNA as template, adopt LA enzymatic amplification intermediate sequence, after product purification, be connected on T carrier, through repeatedly order-checking, obtain complete encoding sequence.With reference to design special primers (table 1) such as Shao Wenting, amplification obtains SmMYB encoding sequence.PCR reaction conditions is: 94 ℃ of 4min; 94 ℃ of 30s, 55 ℃ of 45s, 72 ℃ of 1min (or 2min), 32 circulations; 72 ℃ of 8min.
The TTG1 having logged in the BLASTn method of NCBI and Genbank for the nucleotide sequence that order-checking is obtained, GL3 and TT8 gene order are compared, result shows that SmTTG1 and potato TTG1 gene order homology reach 94%, tomato TTG1 DNA homolog is 92%, SmGL3 and potato GL3 gene order homology reach 94%, tomato GL3 DNA homolog is that 93%, SmTT8 and potato GL3 gene order homology reach 85%, and tomato GL3 DNA homolog is 83%.The clip size that special primer amplification obtains and SmMYB's is in the same size.
The sequence alignment of table 1 eggplant and potato TTG1 gene nucleotide
Potato: potato TTG1 gene nucleotide series (NCBI accession number is XM_006347458)
Eggplant: eggplant TTG1 gene nucleotide series
The aminoacid sequence comparison of table 2 eggplant and potato TTG1 genes encoding
Potato: potato TTG1 gene coding amino acid sequence (NCBI accession number XP_006347519)
Eggplant: eggplant TTG1 gene coding amino acid sequence
The sequence alignment of table 3 eggplant and potato GL3 gene nucleotide
Potato: potato GL3 gene nucleotide series (NCBI accession number is XM_006343915)
Eggplant: eggplant GL3 gene nucleotide series
The aminoacid sequence comparison of table 4 eggplant and potato GL3 genes encoding
Potato: potato GL3 gene coding amino acid sequence (NCBI accession number NP_001275132)
Eggplant: eggplant GL3 gene coding amino acid sequence
The sequence alignment of table 5 eggplant and potato TT8 gene nucleotide
Potato: potato TT8 gene nucleotide series (NCBI accession number is NM_001288169)
Eggplant: eggplant TT8 gene nucleotide order
The aminoacid sequence comparison of table 6 eggplant and potato TT8 genes encoding
Potato: potato TT8 gene coding amino acid sequence (NCBI accession number NP_001275098)
Eggplant: eggplant TT8 gene coding amino acid sequence
Step 2, eggplant SmTTG1, SmGL3, SmTT8 gene organization expression analysis;
Get respectively root, stem, leaf, flower, pericarp and pulp that equivalent has been carried out the eggplant of concentration and quality examination, RNA reverse transcription becomes the best multiple that is diluted to primer work after cDNA, according to Takara test kit
premix ExTagTM (Tli RnaseH Plus) step is carried out fluorescence sxemiquantitative PCR reaction.Using eggplant Actin as internal reference, and design special primer SmAction-F and SmAction-R (subordinate list 1), carry out fluorescence sxemiquantitative PCR to each organ of eggplant and detect.Utilize the FTC-3000 of Funglyn company type quantitative real time PCR Instrument to react, program is as follows:
The step2 that quantitative fluorescent PCR is arranged on stage2 carries out signals collecting.The method of quantitative PCR adopts relative quantification method, by the difference calculating gene expression difference of the Ct value with reference gene ACTIN, and 2
-Δ Δ Ctstandard measure.Quantification PCR primer sequence is in Table 7.Result shows, three genes at root, stem, also, all have expression in flower, pericarp, pulp, but in different tissues expression amount difference.SmTTG1 gene expression level in blade is the highest, is significantly higher than other each tissues; In its hetero-organization, expression amount is suitable, there was no significant difference.The highest other each tissues that is significantly higher than of SmGL3 gene expression amount in stem, are secondly flower and pulp, and in root and leaf, expression amount is very low.The expression amount of SmTT8 in pericarp is the highest, also has remarkable expression in stem, and in root, leaf, flower, pulp, expression amount is low, and there was no significant difference.
Table 7 quantification PCR primer sequence
embodiment 2, the synthetic relevant SmTTG1 of eggplant anthocyanidin, SmGL3, interacts between SmTT8 albumen and SmMYB albumen
The present embodiment relates to the synthetic relevant SmTTG1 of eggplant anthocyanidin, and SmGL3, interacts between SmTT8 albumen and SmMYB albumen, comprises the steps:
Step 1, the structure of yeast two-hybrid recombinant expression vector
Design respectively special primer amplification gene (table 7), use EcoRI and SalI to amplified production SmTTG1, SmGL3, SmTT8 and SmMYB and expression plasmid of yeast pGBKT7 carry out double digestion; With EcoRI and XhoI to amplified production SmTTG1, SmGL3, SmTT8 and SmMYB and Yeast expression carrier pGADT7 carry out double digestion; Four gene fragments are connected with pGBKT7 respectively, and Transformed E .coli DH5 α competent cell is applied to (containing Kana+ resistance) on LB flat board; Four gene fragments are connected with pGAKT7 respectively again, and Transformed E .coli DH5 α competent cell is applied to (containing Amp+ resistance) on LB flat board.37 ℃ of overnight incubation, screening positive recombinant, and carry out bacterium liquid PCR and enzyme is cut evaluation, checks order containing the positive colony Song Sheng work biotech firm that inserts object fragment, identifies that whether Insert Fragment is consistent with goal gene.Recombinant plasmid difference called after pGBKT7-SmTTG1, pGBKT7-SmGL3, pGBKT7-SmTT8, pGADT7-SmTTG1, pGADT7-SmTT8 and pGADT7-SmMYB that sequence is correct.
The cDNA of eggplant of take is template, by synthetic primer amplification respective segments, through 1% agarose gel electrophoresis, detects, and object clip size conforms to the theory amplification value size of primer.The positive colony plasmid DNA of extracting screening, respectively through EcoRI and SalI double digestion, EcoRI and XhoI double digestion, tentatively show after electrophoresis that vector construction is correct.Through company's order-checking comparison, in full accord with goal gene sequence, further prove vector construction success.
Step 2, the conversion of yeast cell
The yeast strain AH109 of-80 ℃ of storages rules on the YPD solid plate that contains 2% glucose, cultivates 2～3 days for 30 ℃.In the YPD liquid nutrient medium that picking list bacterium colony contains 2% glucose in 5mL, 30 ℃ of 220rpm cultivate 24 hours.100 μ L bacterium liquid are transferred in the YPD liquid nutrient medium that 50mL contains 20% glucose, and 30 ℃ of 220rpm cultivate 24 hours.Get 1.5mL bacterium liquid 12000rpm centrifugal 1 minute, abandon after supernatant and add 100 μ L mono-steps to transform the resuspended thalline of damping fluid, add bait, prey, reporter (pSH18-34) carrier and each 100ng of carrier DNA, fully mix.45 ℃ of water-baths after 30 minutes coated plate in SD ∕-Trp ∕-Leu substratum, cultivate about 3 days for 30 ℃.
Step 3, yeast two-hybrid detects SmTTG1, SmGL3, the interaction between SmTT8 and SmMYB albumen
By recombinant plasmid pGBKT7-SmTTG1/pGADT7-SmMYB, pGBKT7-SmGL3/pGADT7-SmMYB, pGBKT7-SmTT8/pGADT7-SmMYB, pGBKT7-SmTT8/pGADT7-SmTTG1, pGBKT7-SmTTG1/pGADT7-SmGL3 and two control group: pGBKT7-SmTTG1/pGADT7, pGBKT7-SmGL3/pGADT7, pGBKT7-SmTT8/pGADT7 control group and pGBKT7/pGADT7-SmTTG1, pGBKT7/pGADT7-SmTT8 and pGBKT7/pGADT7-SmMYB control group, cotransformation is to AH109 competent cell respectively, and method for transformation as above.Converted product is coated respectively SD ∕-Trp ∕-Leu substratum, in 30 ℃, be inverted and cultivate 3-4 days, observe colony growth situation, with the bacterium colony of growing on aseptic toothpick picking flat board, transfer on SD ∕-Trp ∕-Leu/-His and SD ∕-Trp ∕-Leu/-His ∕-Ade substratum, observe it and whether do mutually.
The cotransformation product of recombinant plasmid and the cotransformation product contrasting can be on SD ∕-Trp ∕-Leu substratum normal growth (accompanying drawing 4, the 1st row), but only has recombinant plasmid group pGBKT7-SmTTG1/pGADT7-SmMYB on SD ∕-Trp ∕-Leu/-His ∕-Ade substratum, pGBKT7-SmGL3/pGADT7-SmMYB, pGBKT7-SmTT8/pGADT7-SmMYB, pGBKT7-SmTT8/pGADT7-SmTTG1, pGBKT7-SmTTG1/pGADT7-SmGL3 can grow bacterium colony (accompanying drawing 4, the 4th, 5 row), contrast bait (Bait) group pGBKT7-SmTTG1/pGADT7, pGBKT7-SmGL3/pGADT7, pGBKT7-SmTT8/pGADT7 (accompanying drawing 4, the 2nd is listed as) and contrast prey (Prey) group pGBKT7/pGADT7-SmTTG1, pGBKT7/pGADT7-SmTT8 and pGBKT7/pGADT7-SmMYB do not have growth (Fig. 4, the 3rd row).
Yeast two-hybrid experimental result shows, SmMYB respectively with SmTTG1, SmGL3, exists and interacts between SmTT8, and SmTTG1 and SmTT8, also exists interaction between SmGL3.All cotransformation products can be on two scarce flat boards normal growth and growing way fine, there is not self activation reaction in bait group pGBKT7-SmTTG1, pGBKT7-SmGL3, pGBKT7-SmTT8 simultaneously, and prey group pGADT7-SmTTG1, pGADT7-SmTT8 and pGADT7-SmMYB do not exist self activation reaction yet.
Visible, utilize eggplant SmTTG1 of the present invention, SmGL3, SmTT8 gene, can filter out be subject to its regulation and control with the synthetic closely-related downstream gene of anthocyanidin, and other transcription factor genes interactional with it, thus probe into eggplant anthocyanidin synthesis path regulatory mechanism.Utilize eggplant SmTTG1, SmGL3, SmTT8 gene also can change the gene order in eggplant, thereby eggplant fruit properties is changed by gene is carried out to site mutation, reaches and improves its ornamental value and output, realizes higher economic worth.
The present invention is according to the TTG1 of Solanaceae model plant potato, tomato, petunia and tobacco, GL3, and the gene order that TT8 is relevant, adopts the mode of homologous clone to obtain the SmTTG1 of eggplant, SmGL3, the gene of SmTT8.The cDNA total length 1220bp of SmTTG1 gene, genes encoding head of district 1029bp, does not have intron, 342 amino acid of encoding; The cDNA total length 2329bp of SmGL3 gene, genes encoding head of district 1887bp, 628 amino acid of encoding; The cDNA total length 2242bp of SmTT8 gene, genes encoding head of district 1896bp, 631 amino acid of encoding.
The present invention is synthetic relevant TTG1, GL3 and TT8 gene orders of three kinds of eggplant anthocyanidin of biological technical field.Utilize the method for homologous clone to obtain this full length gene sequence, TTG1 has in SEQ ID NO.1 from the nucleotide sequence shown in Nucleotide 1-1029 position, GL3 has in SEQ ID NO.3 from the nucleotide sequence shown in Nucleotide 1-1887 position, and TT8 has in SEQ ID NO.5 from the nucleotide sequence shown in Nucleotide 1-1896 position.Utilize bioinformatics software to its sequential analysis, determine that SEQ ID NO.1 sequence has typical WD structural domain, be defined as WD40 family gene, SEQID NO.3 and SEQ ID NO.5 sequence all have typical HLH structural domain, determine that they are bHLH family gene.Yeast two-hybrid analysis shows, the SmMYB in eggplant respectively with SmTTG1, SmGL3, SmTT8 interacts, and SmTTG1 and SmTT8, also exists interaction between SmGL3.
Above specific embodiments of the invention are described.It will be appreciated that, the present invention is not limited to above-mentioned specific implementations, and those skilled in the art can make various distortion or modification within the scope of the claims, and this does not affect flesh and blood of the present invention.
Claims (4)
1. an eggplant anthocyanidin synthesis related gene, is characterized in that, described gene is SmTTG1 gene or SmGL3 gene or SmTT8 gene;
The aminoacid sequence of described SmTTG1 genes encoding is as shown in SEQ ID NO.2;
The aminoacid sequence of described SmGL3 genes encoding is as shown in SEQ ID NO.4;
The aminoacid sequence of described SmTT8 genes encoding is as shown in SEQ ID NO.6.
2. eggplant anthocyanidin synthesis related gene as claimed in claim 1, is characterized in that, the sequence of described SmTTG1 gene is as shown in SEQ ID NO.1.
3. eggplant anthocyanidin synthesis related gene as claimed in claim 1, is characterized in that, the sequence of described SmGL3 gene is as shown in SEQ ID NO.3.
4. eggplant anthocyanidin synthesis related gene as claimed in claim 1, is characterized in that, the sequence of described SmTT8 gene is as shown in SEQ ID NO.5.
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| CN104928268A (en) * | 2015-06-16 | 2015-09-23 | 上海交通大学 | Eggplant GDP-D-mannose pyrophosphorylase (SmGMP) protein and encoding genes thereof |
| CN104974992A (en) * | 2015-06-16 | 2015-10-14 | 上海交通大学 | Eggplant GDP-L-galactose phosphatase SmGGP protein and encoding gene |
| CN105087508A (en) * | 2015-06-26 | 2015-11-25 | 上海交通大学 | Eggplant flavanone 3-hydroxylase SmF3H as well as gene and application thereof |
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| CN105440115B (en) * | 2015-12-28 | 2019-02-22 | 上海交通大学 | Eggplant SmHY5 albumen and its encoding gene |
| CN111944772A (en) * | 2020-07-31 | 2020-11-17 | 上海交通大学 | Eggplant cryptochrome blue light inhibitor SmBIC1 protein and coding gene |
| CN111996198A (en) * | 2020-08-26 | 2020-11-27 | 山东农业大学 | Anthocyanin regulatory gene SmbHLH1 in eggplant stem and application thereof |
| CN111996198B (en) * | 2020-08-26 | 2022-06-24 | 山东农业大学 | Anthocyanin regulatory gene SmbHLH1 in eggplant stem and application thereof |
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