CN104045730B - The extracting method of a kind of Semen Nelumbinis polysaccharide - Google Patents
The extracting method of a kind of Semen Nelumbinis polysaccharide Download PDFInfo
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- CN104045730B CN104045730B CN201410292488.XA CN201410292488A CN104045730B CN 104045730 B CN104045730 B CN 104045730B CN 201410292488 A CN201410292488 A CN 201410292488A CN 104045730 B CN104045730 B CN 104045730B
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Abstract
The present invention relates to the extracting method of Semen Nelumbinis polysaccharide, belong to extracted form natural plant technical field. Comprising the steps: to get lotus seeds, add water, making beating, obtains slurries; In slurries, add magnesium sulfate again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate; With water, filtrate is diluted, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane; Clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then uses sodium chloride solution wash-out, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide. The present invention is added in slurries by magnesium sulfate, then is aided with pressure kettle boiling, and the effective constituent in lotus seeds is precipitated more; Pass through ion exchange resin, it is possible to get rid of the impurity in extract, inorganic salt etc.; Concentrated by nanofiltration membrane, it is possible to being effectively separated with inorganic ion by activeconstituents, polysaccharide stays in retaining side, and inorganic salt enter per-meate side, and the purity of product is improved further.
Description
Technical field
The present invention relates to the extracting method of a kind of Semen Nelumbinis polysaccharide, belong to extracted form natural plant technical field.
Background technology
Polysaccharide (polysaccharide) be by multiple monose molecule condensation, dehydration and become, be the complicated and huge glucide of molecule mechanism. All meet macromolecular compound concept carbohydrate and derivative be all called polysaccharide. Polysaccharide is extremely wide, also very important in distributed in nature. Has plenty of the moiety forming animal and plant cells wall, such as peptidoglycan and Mierocrystalline cellulose; Has plenty of the nutrient as animals and plants storage, such as glycogen and starch; What have has special biological activity, has blood coagulation resisting function as the heparin in human body, and the polysaccharide in streptococcus pneumoniae cell walls has antigen effect. The structure unit of polysaccharide is monose, polysaccharide relative molecular mass from several ten thousand to several ten million.
More and more many research finds: the vital process of people nearly all with sugar chain about: such as cell-cell communication, identification and interaction, the motion of cell and effect of adhesion, cause of disease and host cell etc. This is because sugar chain carries bioinformation. It plays decisive role in the molecular recognition process of cell surface.
Four kinds of methods are commonly used in the extraction of polysaccharide: A. hot water extraction method: the destruction carrying out extracting this kind of method extraction polysaccharide according to most of polysaccharide character that solubleness is bigger and stable in the hot water is minimum. B. dilute alkaline aqueous solution extraction method: solubleness is little in the hot water for acidic polysaccharose or the bigger polysaccharide of molecular weight, so conventional 5-15%NaOH or Na2CO3Extraction with aqueous solution. C. enzymolysis process: this method adopts in the preparation of polysaccharide health-care product (such as lentinan healthcare products). But mostly adopting the way that hot water extraction method and enzyme method combine, namely first by hot water extraction, then residue is again by Enzymatic Extraction, and such polysaccharide receipts rate can improve many. D. other method: the organic solvent that the solvent of proton inertia is made solvent extraction polysaccharide an alkali metal salt such as methyl-sulphoxide as lithium chloride-dimethoxy-ethanol extract polysaccharide acidic aqueous solution extract these methods of more than polysaccharide now great majority not have employed, reason is that cost height, receipts rate are low.
Lotus seeds are the seeds of Nymphaeceae aquatic herbaceous plant lotus. Also known as lotus reality, lotus rice, lotus-seed kernel. Lotus, also known as lotus lotus, water sesame. China's most area is all produced, and best with Jianning, Fujian, Guangchang, Jiangxi product person. Four big lotus seeds are carried out nutritive ingredient and have carried out contrast test by Wuhan Inst. of Botany, Chinese Academy of Sciences in 1985, and cun three lotuses in Xiang Tan are all better than other lotus seeds in 17 leading indicators such as amino acid, protein, are described as " Chinese first lotus ". When autumn, fruit maturation in winter, extract lotus pod (seedpod of the lotus), take out fruit; Or get and crash in water, the fruit being sunken in mud, removing shell, using fresh herb or dry use, or peel off crust and the heart (plumule of the cyan) use of lotus seeds, spy is called lotus-seed kernel.
The function of lotus seeds includes: 1. cancer-resisting: lotus seeds are good at tonifying the five internal organs deficiency, logical profit twelve regular channels gas blood, makes gas blood smooth and is stale-proof, and nasopharyngeal carcinoma is had restraining effect by oxoushinsunine contained by lotus seeds, everything, constitute the nutrient health-care function of the cancer-resisting of lotus seeds. 2. blood pressure falls: contained by lotus seeds, non-crystallization shape alkaloid N-9 has hypotensive activity. 3. strong heart and tranquilizing mind: alkaloid contained by embryo nelumbinis has significant cardiotonic, and lotus core alkali then has the effect of stronger anti-calcium and arrhythmia. 4. nourish qi-restoratives, only lose puckery essence: raffinose contained in lotus seeds is all-ages invigorant, for prolonged illness, postpartum or old body void person, commonly use nutrient excellent product especially; Lotus seeds alkali has the effect stabilizing sexual desire, many for young adult's dream, and pass out semen frequent or involuntary emission person, and taking lotus seeds has good puckery essence of only losing to act on. 5. clear away heart-fire, spot of dispelling: band heart lotus seeds energy clearing away the heart-fire, dispel freckle, so can not decoct for a long time.
But, prior art has for the extraction process of Semen Nelumbinis polysaccharide complex process, receipts rate are low, your problem of product purity.
Summary of the invention
It is an object of the invention to: provide that a kind of technique is simple, the extracting method of receipts rate height, Semen Nelumbinis polysaccharide that product purity is high.
Technical scheme:
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, by weight, getting 100��120 parts, lotus seeds, add 200��300 parts, water, making beating, obtains slurries;
2nd step, in slurries, add 10��15 parts, magnesium sulfate again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water by filtrate dilute 2��4 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 3��5wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Further, ion exchange resin refers to D113 Zeo-karb.
Further, in adsorption step, the flow velocity of the feed liquid in bed is 2��4BV/h.
Further, in elution step, the flow velocity of the feed liquid in bed is 1��3BV/h.
Further, the mean pore size of described ceramic microfiltration membrane is 50��500nm.
Further, the molecular weight cut-off of described nanofiltration membrane is 200��400Da.
Further, the boiling temperature of pressure kettle is 110��130 DEG C; Pressure is 0.3��0.5MPa; Cooking time is 0.4��1h.
Useful effect
The present invention is added in slurries by magnesium sulfate, then is aided with pressure kettle boiling, and the effective constituent in lotus seeds is precipitated more; Pass through ion exchange resin, it is possible to get rid of the impurity in extract, inorganic salt etc.;Concentrated by nanofiltration membrane, it is possible to being effectively separated with inorganic ion by activeconstituents, polysaccharide stays in retaining side, and inorganic salt enter per-meate side, and the purity of product is improved further.
Embodiment
Embodiment 1
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 100Kg, add water 200Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 10Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 2 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 3wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 2BV/h; In elution step, the flow velocity of the feed liquid in bed is 1BV/h; The mean pore size of described ceramic microfiltration membrane is 50nm; The molecular weight cut-off of described nanofiltration membrane is 200Da; The boiling temperature of pressure kettle is 110 DEG C; Pressure is 0.3MPa; Cooking time is 0.4h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 3.0%, product purity 34%.
Embodiment 2
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 120Kg, add water 300Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 15Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 4 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 5wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 4BV/h; In elution step, the flow velocity of the feed liquid in bed is 3BV/h; The mean pore size of described ceramic microfiltration membrane is 500nm; The molecular weight cut-off of described nanofiltration membrane is 400Da; The boiling temperature of pressure kettle is 130 DEG C; Pressure is 0.5MPa; Cooking time is 1h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 3.1%, product purity 37%.
Embodiment 3
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 110Kg, add water 2500Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 12Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 3 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 4wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 3BV/h; In elution step, the flow velocity of the feed liquid in bed is 2BV/h; The mean pore size of described ceramic microfiltration membrane is 200nm; The molecular weight cut-off of described nanofiltration membrane is 300Da;The boiling temperature of pressure kettle is 120 DEG C; Pressure is 0.4MPa; Cooking time is 0.6h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 3.8%, product purity 44%.
Embodiment 4
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 105Kg, add water 280Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 14Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 2 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 3wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 4BV/h; In elution step, the flow velocity of the feed liquid in bed is 3BV/h; The mean pore size of described ceramic microfiltration membrane is 50nm; The molecular weight cut-off of described nanofiltration membrane is 400Da; The boiling temperature of pressure kettle is 120 DEG C; Pressure is 0.34MPa; Cooking time is 0.5h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 3.2%, product purity 34%.
Embodiment 5
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 115Kg, add water 230Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 11Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 4 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 5wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 4BV/h; In elution step, the flow velocity of the feed liquid in bed is 3BV/h; The mean pore size of described ceramic microfiltration membrane is 50nm; The molecular weight cut-off of described nanofiltration membrane is 300Da; The boiling temperature of pressure kettle is 120 DEG C; Pressure is 0.45MPa; Cooking time is 0.7h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 3.1%, product purity 31%.
Reference examples 1
It is with the difference of embodiment 3: do not add magnesium sulfate.
1st step, getting lotus seeds 110Kg, add water 2500Kg, making beating, obtains slurries;
2nd step, again slurries are pressure-cooked, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 3 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 4wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 3BV/h; In elution step, the flow velocity of the feed liquid in bed is 2BV/h; The mean pore size of described ceramic microfiltration membrane is 200nm; The molecular weight cut-off of described nanofiltration membrane is 300Da; The boiling temperature of pressure kettle is 120 DEG C;Pressure is 0.4MPa; Cooking time is 0.6h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 1.3%, product purity 29%. It may be seen that the receipts rate of Semen Nelumbinis polysaccharide can be improved significantly by adding magnesium sulfate.
Reference examples 2
Decoct the mode boiled with common normal pressure, and do not adopt pressure kettle.
1st step, getting lotus seeds 110Kg, add water 2500Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 12Kg again, stir evenly, carry out decocting with uncovered container and boil, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 3 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 4wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 3BV/h; In elution step, the flow velocity of the feed liquid in bed is 2BV/h; The mean pore size of described ceramic microfiltration membrane is 200nm; The molecular weight cut-off of described nanofiltration membrane is 300Da; Decocting time is 0.6h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 1.7%, product purity 26%. Semen Nelumbinis polysaccharide can be made better to be suggested it may be seen that adopt high pressure to decoct the mode boiled.
Reference examples 3
It is with the difference of embodiment 3: do not carry out adsorption and desorption by ion exchange resin.
The extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 110Kg, add water 2500Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 12Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 3 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, by clear for ceramic membrane liquid use nanofiltration membrane concentrate, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide.
The mean pore size of described ceramic microfiltration membrane is 200nm; The molecular weight cut-off of described nanofiltration membrane is 300Da; The boiling temperature of pressure kettle is 120 DEG C; Pressure is 0.4MPa; Cooking time is 0.6h.
By the weight of lotus seeds, the receipts rate of the Semen Nelumbinis polysaccharide obtained is 2.6%, product purity 20%. It may be seen that by the absorption, the wash-out that carry out ion exchange resin, it is possible to effectively improve the purity of product.
Claims (1)
1. the extracting method of Semen Nelumbinis polysaccharide, comprises the steps:
1st step, getting lotus seeds 120Kg, add water 300Kg, making beating, obtains slurries;
2nd step, in slurries, add magnesium sulfate 15Kg again, stir evenly, pressure-cook, after letting cool, sieve, obtain filtrate;
3rd step, with water, filtrate is diluted 4 times, filter with ceramic microfiltration membrane, obtain the clear liquid of ceramic membrane;
4th step, clear for ceramic membrane liquid spent ion exchange resin is adsorbed, then with the sodium chloride solution wash-out of 5wt.%, use nanofiltration membrane to concentrate at elutriant, after being dried by concentrated solution, obtain Semen Nelumbinis polysaccharide;
Ion exchange resin refers to D113 Zeo-karb; In adsorption step, the flow velocity of the feed liquid in bed is 4BV/h; In elution step, the flow velocity of the feed liquid in bed is 3BV/h; The mean pore size of described ceramic microfiltration membrane is 500nm; The molecular weight cut-off of described nanofiltration membrane is 400Da;The boiling temperature of pressure kettle is 130 DEG C; Pressure is 0.5MPa; Cooking time is 1h.
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| CN104628881A (en) * | 2015-03-05 | 2015-05-20 | 四川省农业科学院生物技术核技术研究所 | Method for extracting auricularia polytricha polysaccharide |
| CN105348403B (en) * | 2015-10-15 | 2018-06-15 | 西北师范大学 | A kind of method that ultrawhite lily polysaccharide is extracted in inferior lily bud scale |
| CN108753872B (en) * | 2018-07-06 | 2020-09-25 | 福建师范大学福清分校 | High-yield extraction method of lotus seed polysaccharide |
| CN112006281A (en) * | 2020-08-23 | 2020-12-01 | 赣州禾绿康健生物技术有限公司 | Preparation device and process of micromolecular ginseng syrup |
| CN112390897B (en) * | 2020-11-24 | 2022-09-30 | 邯郸学院 | Method for extracting and separating high-purity polysaccharide from plant flowers |
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| CN101385909A (en) * | 2008-09-12 | 2009-03-18 | 天津科技大学 | Extraction method of natural soluble polysaccharide using radial chromatography |
| CN102382203A (en) * | 2011-09-19 | 2012-03-21 | 福建闽江源绿田实业投资发展有限公司 | High-efficiency extraction process for polysaccharide of lotus seeds |
| CN103387620A (en) * | 2013-08-08 | 2013-11-13 | 福建中医药大学 | Polysaccharide, total flavonoid and total alksloid prepared from lotus plumule and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385909A (en) * | 2008-09-12 | 2009-03-18 | 天津科技大学 | Extraction method of natural soluble polysaccharide using radial chromatography |
| CN102382203A (en) * | 2011-09-19 | 2012-03-21 | 福建闽江源绿田实业投资发展有限公司 | High-efficiency extraction process for polysaccharide of lotus seeds |
| CN103387620A (en) * | 2013-08-08 | 2013-11-13 | 福建中医药大学 | Polysaccharide, total flavonoid and total alksloid prepared from lotus plumule and preparation method thereof |
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