CN104026013B - Sterile disinfection device for surfaces of plant tissues - Google Patents
Sterile disinfection device for surfaces of plant tissues Download PDFInfo
- Publication number
- CN104026013B CN104026013B CN201410269159.3A CN201410269159A CN104026013B CN 104026013 B CN104026013 B CN 104026013B CN 201410269159 A CN201410269159 A CN 201410269159A CN 104026013 B CN104026013 B CN 104026013B
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- China
- Prior art keywords
- disinfector
- axle
- fungi
- disinfectors
- blade
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 25
- 230000006835 compression Effects 0.000 claims abstract description 14
- 238000007906 compression Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000000645 desinfectant Substances 0.000 claims description 9
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 abstract 3
- 241000894006 Bacteria Species 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 5
- 238000004161 plant tissue culture Methods 0.000 description 4
- 230000000739 chaotic effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sterile disinfection device for surfaces of plant tissues. According to the device, disinfector shafts and a plurality of independent disinfectors are respectively arranged among groove-shaped vertical plates and are hinged with anti-bacterial plate hinging shafts and anti-bacterial plates; sample grooves are formed in the bottom surfaces of the vertical plates and are communicated with liquid injecting holes; fixed compression bars and cutting blades which can move up and down are arranged in the disinfectors; the sample grooves corresponding to the lower parts of the disinfectors are respectively shielded by the independent anti-bacterial plates and are mutually isolated, so that plant samples can be simultaneously fixed in batches, i.e., the unified comprehensive sterilization can be carried out. Thus, the operation processes are reduced; the time is saved; the disinfection effects on the surfaces of the plant samples are improved; and the plant samples can be accurately numbered, so that the disordered sequence is avoided. The small batch treatment and the isolated bacteria control for the plant samples are carried out in the independent disinfectors, so that the secondary pollution caused by long operation time and complicated operations in subsequent separation and culture operations is effectively avoided. As a result, various defects in the existing traditional disinfection effect are solved.
Description
Technical field
The present invention relates to a kind of Aseptic sterilisation device, specifically a kind of plant tissue surface Aseptic sterilisation device, be applicable to the plant tissue surface Aseptic sterilisation before microorganism separation and Culture in plant tissue culture, the plant tissues such as microbiology, forest, agronomy, belong to biological technical field.
Background technology
In the separation and Culture process of microorganism in plant tissue culture and plant tissue, in order to obtain desirable pure culture, surface sterilization process thoroughly must be carried out to plant tissue sample, the eternal loss of activity of the microorganism that its surface is carried, and in follow-up operation, avoid the secondary pollution of plant sample, this is the key directly having influence on microorganism separation and Culture success or not in plant tissue culture and plant tissue.
Traditional plant tissue surface aseptic sterilisation methods be adopt the single mode processed respectively, namely with tweezers one by one gripping plant sample put into medicining liquid dipping sterilizing, then use sterilized water washes clean.Whole operating process is too loaded down with trivial details and consuming time, cause work efficiency degradation, especially when plant sample is more, gripping plant sample implements a large amount of single palikinesia frequently, not only inefficiency, sterilization effect is unstable, also easily causes sample number chaotic, or cause the serious consequence of secondary pollution, directly affect the success rate of microorganism separation and Culture in plant tissue culture and plant tissue.Still do not have a kind of Aseptic sterilisation device can improve the efficiency of plant tissue surface Aseptic sterilisation at present, with serious pollution problem can be avoided preferably again.
Summary of the invention
The object of the invention is to solve the loaded down with trivial details and problem of sterilization effect instability of current traditional plant tissue surface aseptic sterilisation methods complicated operation, and a kind of plant tissue surface Aseptic sterilisation device is provided.This device not only can batch process is a large amount of quickly and easily plant sample to be sterilized, effectively can also avoid the secondary pollution in operating process, improve sterilization effect.
The technical scheme realizing the object of the invention is as follows:
The vertical facade at grooved bottom board two ends is respectively equipped with disinfector track, be hinged between the disinfector track at two ends disinfector axle, disinfector axle two ends are respectively provided with adjusting nut, disinfector axle is connected several disinfectors, also be hinged between the vertical facade in two ends fungi-proofing plate hinge, fungi-proofing plate hinge is hinged several fungi-proofing plates, position corresponding with disinfector on grooved bottom board bottom surface is respectively equipped with sample slot, disinfectant solution is marked with in it, liquid injection hole is positioned at grooved bottom board edge and the position of close sample slot, between each sample slot and mutually through between sample slot and liquid injection hole, compression column with fixed movable up and down and cutting blade is provided with in disinfector, cutting blade cover is connected in the right and left and is provided with on the blade slides axle of blade spring, by spring tension, cutting blade is expressed to the upper end of blade slides axle, the top of cutting blade extends to disinfector and connects blade hands handle outward, compression column with fixed cover is connected in the right and left and is provided with on the depression bar sliding axle of Pressure-bar spring, by spring tension, compression column with fixed is expressed to the lower end of depression bar sliding axle, sample slot corresponding to each disinfector and bottom all has independent fungi-proofing plate to cover, and isolate with adjacent overlay area.
The present invention can fix plant sample mass simultaneous, carries out the comprehensive sterilizing of unifying, not only increases the effect of plant sample surface sterilization, has saved a large amount of time, can also unify mark, accurately number plant sample, avoids order chaotic.In this external independently disinfector, a small amount of batch processing carried out to plant sample and isolate fungi-proofing, can also effectively to avoid in the operation of follow-up separation and ientification because the operating time is long, frequent operation and the secondary pollution phenomenon that causes, efficiently solve in current conventional sterilization methods the various drawbacks existed.
Accompanying drawing explanation
Accompanying drawing 1 is that the master of the embodiment of the present invention looks part section structural representation.
Accompanying drawing 2 is plan structure schematic diagrams of the embodiment of the present invention.
Accompanying drawing 3 is right TV structure schematic diagrams of the embodiment of the present invention.
Accompanying drawing 4 are embodiment of the present invention accompanying drawings 3 along A-A to generalized section.
Accompanying drawing 5 are embodiment of the present invention accompanying drawings 3 along B-B to generalized section.
Description of reference numerals is as follows:
Grooved bottom board 1, disinfector 2, disinfector track 3, fungi-proofing plate 4, fungi-proofing plate hinge 5, disinfector axle 6, adjusting nut 7, compression column with fixed 8, depression bar sliding axle 9, Pressure-bar spring 10, cutting blade 11, blade handle 12, blade slides axle 13, blade spring 14, liquid injection hole 15, sample slot 16, disinfectant solution 17, plant sample 18.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, technical scheme of the present invention is described in further detail:
The vertical facade at grooved bottom board (1) two ends is respectively equipped with disinfector track (3), be hinged between the disinfector track (3) at two ends disinfector axle (6), disinfector axle (6) two ends are respectively provided with adjusting nut (7), disinfector axle (6) is connected three disinfectors (2), also be hinged between the vertical facade in two ends fungi-proofing plate hinge (5), upper hinge three the fungi-proofing plates (4) of fungi-proofing plate hinge (5), position corresponding with disinfector (2) on grooved bottom board (1) bottom surface is respectively equipped with sample slot (16), disinfectant solution (17) is marked with in it, liquid injection hole (15) is positioned at grooved bottom board (1) edge and the position of close sample slot (16), between each sample slot (16) and mutually through between sample slot (16) and liquid injection hole (15), compression column with fixed movable up and down (8) and cutting blade (11) is provided with in disinfector (2), cutting blade (11) cover is connected in the right and left and is provided with on the blade slides axle (13) of blade spring (14), by spring tension, cutting blade (11) is expressed to the upper end of blade slides axle (13), the top of cutting blade (11) extends to disinfector (2) and connects blade handle (12) outward, compression column with fixed (8) cover is connected in the right and left and is provided with on the depression bar sliding axle (9) of Pressure-bar spring (10), by spring tension, compression column with fixed (8) is expressed to the lower end of depression bar sliding axle (9), the bottom of compression column with fixed (8) is dentation, sample slot (16) corresponding to each disinfector (2) and bottom all has independent fungi-proofing plate (4) to cover, and isolate with adjacent overlay area.
Before the present invention uses, adopt autoclaving to carry out thorough sterilizing to this device, follow-up all operations all carries out in aseptic superclean bench.First unscrew adjusting nut and by disinfector horizontal slip to the one end away from sample slot, lightly plant tissue sample be placed in sample slot in order one by one with tweezers, determine the sample size of placing in each sample slot according to the size of plant sample.Subsequently disinfector is moved to vertical rail slide downward, plant sample is gripped between grooved bottom board and fixing tabletting, adjusting nut is screwed after compression, preventing from loosening causes plant sample to be lost, and cover fungi-proofing plate, labelling sample number on fungi-proofing plate, then draw a certain amount of disinfectant solution and injected by liquid injection hole, guarantee there are enough disinfectant solution in each sample slot, can all plant samples of submergence completely.Sterilization time is determined according to the difference of plant sample and sterilisation purpose, as being separated the tip of a root surface sterilization of Applying Ectomycorrhizal Fungi from the tip of a root, the tip of a root need be soaked 3-4 minute in disinfectant solution, last rapidly by disinfectant solution by liquid injection hole sucking-off, and use the same method and inject sterilized water by plant sample repeated washing 3-4 time, to remove the disinfectant solution of its remained on surface.After having sterilized, as far as possible by whole for cleaning mixture sucking-off, and raise fungi-proofing plate one by one, plant sample cuts off by pressure cutting blade, with tweezers one by one gripping plant sample carry out follow-up separation and cultivate operation.After operation terminates, this device is cleaned up in order to reusing, later all must sterilizing before each use.
Claims (2)
1. a plant tissue surface Aseptic sterilisation device, it is characterized in that: on the vertical facade at grooved bottom board (1) two ends, be respectively equipped with disinfector track (3), be hinged between the disinfector track (3) at two ends disinfector axle (6), disinfector axle (6) two ends are respectively provided with adjusting nut (7), disinfector axle (6) is connected several disinfectors (2), also be hinged between the vertical facade in two ends fungi-proofing plate hinge (5), upper several the fungi-proofing plates (4) that are hinged of fungi-proofing plate hinge (5), position corresponding with disinfector (2) on grooved bottom board (1) bottom surface is respectively equipped with sample slot (16), disinfectant solution (17) is marked with in it, liquid injection hole (15) is positioned at grooved bottom board (1) edge and the position of close sample slot (16), between each sample slot (16) and mutually through between sample slot (16) and liquid injection hole (15), compression column with fixed movable up and down (8) and cutting blade (11) is provided with in disinfector (2), cutting blade (11) cover is connected in the right and left and is provided with on the blade slides axle (13) of blade spring (14), by spring tension, cutting blade (11) is expressed to the upper end of blade slides axle (13), the top of cutting blade (11) extends to disinfector (2) and connects blade handle (12) outward, compression column with fixed (8) cover is connected in the right and left and is provided with on the depression bar sliding axle (9) of Pressure-bar spring (10), by spring tension, compression column with fixed (8) is expressed to the lower end of depression bar sliding axle (9), the bottom of compression column with fixed (8) is dentation, sample slot (16) corresponding to each disinfector (2) and bottom all has independent fungi-proofing plate (4) to cover, and isolate with adjacent overlay area.
2. a kind of plant tissue surface Aseptic sterilisation device according to claim 1, is characterized in that: disinfector axle (6) is connected three disinfectors (2), upper hinge three the fungi-proofing plates (4) of fungi-proofing plate hinge (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410269159.3A CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410269159.3A CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104026013A CN104026013A (en) | 2014-09-10 |
| CN104026013B true CN104026013B (en) | 2015-07-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410269159.3A Expired - Fee Related CN104026013B (en) | 2014-06-17 | 2014-06-17 | Sterile disinfection device for surfaces of plant tissues |
Country Status (1)
| Country | Link |
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| CN (1) | CN104026013B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111226795B (en) * | 2020-03-06 | 2022-12-30 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Sterilization device for floating samples |
| CN113287523A (en) * | 2021-06-08 | 2021-08-24 | 陈嘉裔 | Intermittent fast-immersion succulent plant propagation and disinfection device and implementation method thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01158957A (en) * | 1987-12-11 | 1989-06-22 | Komatsu Ltd | Sterilizing apparatus |
| JPWO2004114378A1 (en) * | 2003-06-19 | 2006-07-27 | 北海道ティー・エル・オー株式会社 | CLEAN UNIT, CLEAN UNIT SYSTEM, FUNCTIONAL UNIT, FUNCTIONAL UNIT SYSTEM, MATERIAL PROCESSING METHOD, ELEMENT MANUFACTURING METHOD, CELL SYSTEM GROWING METHOD AND PLANT BODY GROWING METHOD |
| CN201453700U (en) * | 2009-06-05 | 2010-05-12 | 重庆大学 | Explant surface sterilizing device |
| CN102018969B (en) * | 2010-11-30 | 2013-07-03 | 上海交通大学 | Device for disinfecting surfaces of explants of plants |
| CN102166367B (en) * | 2010-12-30 | 2013-07-10 | 西北农林科技大学 | Method for AMF spore surface disinfection |
| CN202459434U (en) * | 2011-12-02 | 2012-10-03 | 刘家迅 | Plant tissue explant sterilizing device |
| CN203538050U (en) * | 2013-09-16 | 2014-04-16 | 上海市农业科学院 | Cleaning sterilizer for tissue culture explant of aquatic plant |
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2014
- 2014-06-17 CN CN201410269159.3A patent/CN104026013B/en not_active Expired - Fee Related
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| CN104026013A (en) | 2014-09-10 |
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Granted publication date: 20150708 |