CN104014811B - A kind of octreotide acetate that utilizes is for the method for Template preparation coralloid nano cobalt - Google Patents
A kind of octreotide acetate that utilizes is for the method for Template preparation coralloid nano cobalt Download PDFInfo
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- CN104014811B CN104014811B CN201410234741.6A CN201410234741A CN104014811B CN 104014811 B CN104014811 B CN 104014811B CN 201410234741 A CN201410234741 A CN 201410234741A CN 104014811 B CN104014811 B CN 104014811B
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- octreotide acetate
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Abstract
A kind of octreotide acetate that utilizes is for the method for Template preparation coralloid nano cobalt, does it mainly use pH? 2 ~ 3 dissolving with hydrochloric acid octreotide acetates, octreotide acetate is adjusted to acid solution, be the ratio of 1:10 in octreotide acetate and metal salt solution mol ratio again, cobalt chloride solution is added in the solution that above-mentioned conciliation is good, and put it in water-bath constant temperature oscillator, in 100 ~ 200rpm, at 13 ~ 25 DEG C, hatch 20 ~ 26h; Then be the ratio of 1:2 or 1:3 or 1:5 in the mol ratio of metal salt solution and reducing agent, disposablely in above-mentioned solution of hatching, add borane reducing agent sodium hydride, make it become aterrimus from pale pink, this aterrimus product is octreotide acetate-nanometer cobalt particle.The present invention has that preparation technology is simple, mild condition, and cheap and easy to get, reaction is easy to control, productive rate advantages of higher.
Description
Technical field
The invention belongs to nano metal material technical field, particularly a kind of preparation method of nano material.
Background technology
Nanometer cobalt particle has many purposes at industrial circles such as electronics industry, magnetic, carbide alloy, surface spraying, chemical catalysis as high-performance magnetism recording materials, magnetic fluid, absorbing material, activated sintering additive etc.
The preparation method of nanometer cobalt particle has thermal decomposition method, precipitation-thermal decomposition method, hydrogen reduction method, microemulsion method etc.Some technical requirement of above-mentioned preparation method is high, apparatus expensive, some complex process, and environmental pollution is comparatively large, and some needs longer reaction time, higher temperature and pressure and special device anyway, causes production cost higher.In recent years, what the method preparing nanometer cobalt was comparatively enlivened is liquid phase reduction, but the nano cobalt granule particle diameter of existing liquid phase reduction preparation technology gained is comparatively large, and domain size distribution is wider, and products therefrom is easily reunited, and is difficult to extensive use.
Summary of the invention
The object of the present invention is to provide that a kind of preparation cost is cheap, pollution-free, technique be simple, controlled utilizes octreotide acetate for the method for Template preparation coralloid nano cobalt, solve that liquid phase reduction can not obtain that particle diameter is little, the difficult problem of good dispersion and the single nano cobalt granule of composition.The present invention mainly adopts octreotide acetate molecule to be template, what utilize biomolecule to have improves and strict molecular recognition function, by physico-chemical process according to the avtive spot located growth nano particle of designing requirement on its surface, simultaneously the architectural feature of biomolecule self and space confinement effect thereof accurately can control the size of nano particle and pattern, thus obtain the coralloid nano cobalt particle of expecting.
Technical scheme of the present invention is as follows:
(1) getting octreotide acetate, is that 2 ~ 3 hydrochloric acid solutions dissolve 0.4 ~ 0.8mg octreotide acetate with every ml of ph, is mixed with the acid octreotide acetate solution of 0.4 ~ 0.8mM that pH value is 2 ~ 3;
(2) be the ratio of 1:10 in octreotide acetate and metal salt solution mol ratio, cobalt chloride solution is added in the octreotide acetate solution that step (1) has been reconciled, preferably add the cobalt chloride solution with octreotide acetate solution same volume, and put it in water-bath constant temperature oscillator, in 100 ~ 200rpm., at 13 ~ 25 DEG C, hatch 20 ~ 26h;
(3) in the mol ratio of metal salt solution and reducing agent be the ratio of 1:2 or 1:3 or 1:5, disposablely in the solution of dropwise hatching to step (2) add borane reducing agent sodium hydride, make it become aterrimus from pale pink, this aterrimus product is octreotide acetate-nanometer cobalt particle.
The present invention compared with prior art tool has the following advantages:
1, the present invention adopts octreotide acetate to be template, its molecular formula is H-D-Phe-Cys-Phe-D-Trp-Lys-Thr-Cys-Thr-OL (Cys2-Cys7), its molecular structure is simple, active group is clear, be easy to analysis and control, and pass through electrostatic interaction, object ion is easy to the surface-active site being adsorbed onto octreotide acetate, by controlling reduction rate and reducing degree, and then generate the nanometer cobalt particle of pattern rule, so octreotide acetate has the characteristic of the excellent biological template of the homogeneous nanometer cobalt particle of preparation.
The good dispersion of 2, obtained coralliform cobalt nanoparticle, pattern is typical, form is regular, structure-controllable.
3, preparation technology is simple, mild condition, and preparation cost is cheap, and course of reaction is easy to control, and is easy to obtain expected results, low to the requirement of equipment.
4, the reducing agent adopted, reaction medium and reactant price are low, pollution-free.
Accompanying drawing explanation
Fig. 1 is the TEM figure of octreotide acetate-nanometer cobalt particle that the embodiment of the present invention 1 obtains.
Fig. 2 is the TEM figure of octreotide acetate-nanometer cobalt particle that the embodiment of the present invention 2 obtains.
Fig. 3 is the EDS figure of octreotide acetate-nanometer cobalt particle that the embodiment of the present invention 2 obtains.
Fig. 4 is the TEM figure of octreotide acetate-nanometer cobalt particle that the embodiment of the present invention 3 obtains.
Detailed description of the invention
Embodiment 1
Get the octreotide acetate (the biochemical Shanghai Co., Ltd of gill produces) of 0.4mg, be dissolved in the hydrochloric acid solution of 1mLpH2, make the pH value of octreotide acetate solution be 2; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L4mM; The above-mentioned solution prepared is put into water-bath constant temperature oscillator, in 100rpm., after hatching 26h at 13 DEG C, the disposable 50mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 80 μ L reduce, make it become aterrimus from pale pink, namely obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 1, the diameter of coralloid nano cobalt particle is about 55nm, and pattern rule, is evenly distributed.
Embodiment 2
Get the octreotide acetate (the biochemical Shanghai Co., Ltd of gill produces) of 0.6mg, be dissolved in the hydrochloric acid solution of 1mLpH2.5, make the pH value of octreotide acetate solution be 2.5; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L6mM; The above-mentioned solution prepared is put into water-bath constant temperature oscillator, in 150rpm., after hatching 24h at 20 DEG C, the disposable 40mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 90 μ L reduce, make it become aterrimus from pale pink, namely obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 2, the diameter of coralloid nano cobalt particle is about 60-70nm, and pattern rule, is evenly distributed.
Application energy disperse spectroscopy characterizes coralloid nano cobalt particle, as inscribed shown in 3, the peak that Co, C, O, Cl element is corresponding is there is in power spectrum, the metallic element of description taken in conjunction on octreotide acetate surface is cobalt, and O element is the element in albumen, and C element is the element in copper mesh, in addition also some C element can be there are in albumen, illustrate that octreotide acetate is combined well with cobalt particle, and purity is very high, Cl element carrys out autoreduction not cobalt chloride thoroughly.
Embodiment 3
Get the octreotide acetate (the biochemical Shanghai Co., Ltd of gill produces) of 0.8mg, be dissolved in the hydrochloric acid solution of 1mLpH3, make the pH value of octreotide acetate solution be 3; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L8mM; The above-mentioned solution prepared is put into water-bath constant temperature oscillator, in 200rpm., after hatching 20h at 25 DEG C, the disposable 32mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 100 μ L reduce, make it become aterrimus from pale pink, namely obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 4, the diameter of coralloid nano cobalt particle is about 40-50nm, and pattern rule, is evenly distributed.
Claims (2)
1. utilize octreotide acetate for a method for Template preparation coralloid nano cobalt, it is characterized in that:
(1) getting octreotide acetate, is that 2 ~ 3 hydrochloric acid solutions dissolve 0.4 ~ 0.8mg octreotide acetate with every ml of ph, is mixed with the acid octreotide acetate solution of 0.4 ~ 0.8mM that pH value is 2 ~ 3;
(2) be the ratio of 1:10 in octreotide acetate and metal salt solution mol ratio, cobalt chloride solution is added in the solution that step (1) has been reconciled, and put it in water-bath constant temperature oscillator, in 100 ~ 200rpm., at 13 ~ 25 DEG C, hatch 20 ~ 26h;
(3) in the mol ratio of metal salt solution and reducing agent be the ratio of 1:2 or 1:3 or 1:5, borane reducing agent sodium hydride is added in disposable solution of hatching to step (2), make it become aterrimus from pale pink, the coralloid nano cobalt particle that monodispersity is good can be obtained.
2. the octreotide acetate that utilizes according to claim 1 is for the method for Template preparation coralloid nano cobalt, it is characterized in that: add the cobalt chloride solution with octreotide acetate solution same volume.
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| CN201410234741.6A CN104014811B (en) | 2014-05-29 | 2014-05-29 | A kind of octreotide acetate that utilizes is for the method for Template preparation coralloid nano cobalt |
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| US9849512B2 (en) | 2011-07-01 | 2017-12-26 | Attostat, Inc. | Method and apparatus for production of uniformly sized nanoparticles |
| US9434006B2 (en) | 2014-09-23 | 2016-09-06 | Attostat, Inc. | Composition containing spherical and coral-shaped nanoparticles and method of making same |
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| US9885001B2 (en) * | 2014-09-23 | 2018-02-06 | Attostat, Inc. | Fuel additive composition and related methods |
| WO2016161348A1 (en) | 2015-04-01 | 2016-10-06 | Attostat, Inc. | Nanoparticle compositions and methods for treating or preventing tissue infections and diseases |
| US11473202B2 (en) | 2015-04-13 | 2022-10-18 | Attostat, Inc. | Anti-corrosion nanoparticle compositions |
| WO2016168346A1 (en) | 2015-04-13 | 2016-10-20 | Attostat, Inc. | Anti-corrosion nanoparticle compositions |
| CN105312591B (en) * | 2015-11-13 | 2017-04-26 | 燕山大学 | Method for preparing annular platinum nanoparticles with lanreotide acetate serving as template |
| US10201571B2 (en) | 2016-01-25 | 2019-02-12 | Attostat, Inc. | Nanoparticle compositions and methods for treating onychomychosis |
| CN106735289B (en) * | 2016-11-30 | 2018-09-04 | 燕山大学 | A method of preparing cubic silver nanoparticle box by template of octreotide acetate |
| US11018376B2 (en) | 2017-11-28 | 2021-05-25 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
| US11646453B2 (en) | 2017-11-28 | 2023-05-09 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
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| CN102784925A (en) * | 2012-07-16 | 2012-11-21 | 燕山大学 | Method for preparing gold nano particle chains in aqueous phase by taking octreotide acetate as template |
| CN102784924A (en) * | 2012-07-16 | 2012-11-21 | 燕山大学 | Water phase preparation method for chain platinum nanosphere by taking octreotide acetate as template |
| CN103055893A (en) * | 2012-12-25 | 2013-04-24 | 北京化工大学 | Supported cobalt-platinum superlattice alloy nano particle composite catalyst and preparation method thereof |
| CN103203461A (en) * | 2013-03-21 | 2013-07-17 | 燕山大学 | Method for preparing hammer-shaped palladium nanoparticle by utilizing octreotide acetate as template |
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| EP2132013B1 (en) * | 2007-03-27 | 2012-02-08 | Ludmilla Morozova-Roche | Method for the manufacture of thin metal nanowires by biotemplating |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102784925A (en) * | 2012-07-16 | 2012-11-21 | 燕山大学 | Method for preparing gold nano particle chains in aqueous phase by taking octreotide acetate as template |
| CN102784924A (en) * | 2012-07-16 | 2012-11-21 | 燕山大学 | Water phase preparation method for chain platinum nanosphere by taking octreotide acetate as template |
| CN103055893A (en) * | 2012-12-25 | 2013-04-24 | 北京化工大学 | Supported cobalt-platinum superlattice alloy nano particle composite catalyst and preparation method thereof |
| CN103203461A (en) * | 2013-03-21 | 2013-07-17 | 燕山大学 | Method for preparing hammer-shaped palladium nanoparticle by utilizing octreotide acetate as template |
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