CN104012267A - Maize salt tolerance identification method - Google Patents
Maize salt tolerance identification method Download PDFInfo
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- CN104012267A CN104012267A CN201410183121.4A CN201410183121A CN104012267A CN 104012267 A CN104012267 A CN 104012267A CN 201410183121 A CN201410183121 A CN 201410183121A CN 104012267 A CN104012267 A CN 104012267A
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Abstract
The invention provides a maize salt tolerance identification method. According to the method, the salt tolerance of materials is evaluated by comparing the fresh weights of the overground parts, the dry weights of the overground parts and the K+/Na+ ion content ratios of different materials which are subjected to salt stress. The experiment period is short, one month is enough from the start of treatment to the end of investigation, and the problem of long period of field identification is avoided. Operation is easy, identification indices only include material weights and ion contents, and the method is suitable for large-scale maize material identification. Furthermore, the fresh weights of the overground parts, the dry weights of the overground parts and the K+/Na+ ion content ratios are adopted as the identification indices to conduct mutual verification with the overground part dry weight and fresh weight indices, and manual operating errors are avoided. The method has the advantages of being accurate and reliable.
Description
Technical field
The present invention relates to plant physiology, specifically, relate to a kind of salt tolerance of corn authentication method.
Background technology
The soil salinization, as main abiotic stress, has a strong impact on world's crop yield.8,300,000 hectares of the global gross areas of whole world saline soil, wherein approximately 20% belongs to irrigated farmland, and salination has the trend of further aggravation.Along with world's total population constantly increases, particularly, part less developed country and some newly industrializing countries, the contradiction of population growth and available Cultivated Land Area Decrease is increasingly sharpened.Therefore, the improvement of crops salt resistance seems particularly important.
The saline and alkaline impact on crop mainly contains: osmotic stress, ion are poisoned and ion metabolism disorder.In saliferous soil, plant absorbs Na by water shunting in root system
+, K
+deng salt ion, these ions are by collaborative each tissue of plant that is transported to, in cell, the ion of high concentration causes the inside and outside permeable pressure head of cell membrane, replacement that too much sodium ion and chlorion causes cytotoxic and sodium ion simultaneously, potassium ion is affine, plant will show wilts and biological arid, finally causes biological yield and economic flow rate to decline.At occurring in nature, have various abiotic stress, and plant forms a set of special Mechanisms of Salt Resistance in long-term evolution process, comprise outer excretion salt, salt ion compartmentation, retardance transportation etc.And for species, due to the difference of its genetic evolution, individual all have certain hereditary difference aspect salt resistance, these hereditary difference external manifestations are exactly the parameter variation relevant to Physiology and biochemistry.Therefore, select a set of Salt-Tolerance Identification method easily repeatably reliably, significant for crops breeding for stress tolerance.
Corn is as the generalized grain crop that beats the world, significant to world food safety, and corn be one to salt-sensitive crop, once corn is subject to after salt stress, shows blade and wilt, the top albefaction of blade, plant development is slow, and biological yield declines, and finally causes Severe Reduction.In recent years, corn breeding develops rapidly, and its resistance is paid attention to more, and domestic also relatively less for Recent Progress in Study on Salt Tolerance of Corn, therefore sets up a set of salt tolerance of corn and identifies that reliable method is significant.
Summary of the invention
The object of this invention is to provide a kind of method of fast, precisely identifying salt tolerance of corn.
In order to realize the object of the invention, a kind of salt tolerance of corn authentication method of the present invention, comprises the following steps:
1) corn seed is germinateed on germination paper, choose and sprout consistent seedling;
2) seedling replanting is entered to be equipped with in the flowerpot of vermiculite, before two leaf stage, water the Hoagland nutrient solution of 1/2 concentration;
3) seedling is after two leaf stage, and control group plant continues the Hoagland nutrient solution of pouring 1/2 concentration, and processed group plant is watered respectively the NaCl salting liquid of 100mM and 200mM, and the next day waters once, until wilting phenotype appears in plant material;
4) plant occurs after wilting phenotype, getting seedling acrial part weighs, be placed in again 85-160 ℃ of (preferably 85 ℃) baking oven dry 36-48 hour (preferably 48 hours), again weigh, the digestive juice that the material of oven dry is placed in to 10 times of volumes of material is processed 8-12 hour (preferably 12 hours), then digestive juice is placed in to muffle furnace in 175-185 ℃ of heating 2-3 hour, until that solution becomes is as clear as crystal, by after above solution dilution, on Atomic Absorption Spectrometer, measure Na
+, K
+ion concentration.Described digestive juice is HNO
3and HClO
4press 4:1 volume ratio mixed preparing.
Wherein, step 2) and 3) condition of culture be: 25 ℃ of illumination 16 hours, intensity of illumination is 450 μ molm
-2s
-1, 23 ℃ of dark 8 hours, relative moisture 65%.
Particularly, rice Salt-Tolerance Identification method of the present invention comprises the following steps:
1) material is prepared: collect the seed of different corn materials, and 3 processing of each material, each processes 3 repetitions, and each repeats to plant 4 strains, with after deionized water rinsing, is placed on germination paper, and 28 ℃ germinate 48 hours.
2) seedling replanting and cultivation: by sprouting consistent seed, be transplanted in the small flower that vermiculite is housed, treat that whole seedling sowings are complete, flowerpot is placed in incubator, condition of culture is: 25 ℃ of illumination 16 hours, intensity of illumination is 450 μ molm
-2s
-1, 23 ℃ of dark 8 hours, relative moisture 65%.Before two leaf stage, seedling is watered the Hoagland nutrient solution (Hoagland & Arnon, 1950) of 1/2 concentration.
3) salt stress is processed: until seedling the second blade open and flat after, control group plant pouring Hoagland nutrient solution, the seedling of Stress treatment group is watered respectively 100mM and 200mM NaCl solution, the next day, waters once, until wilting phenotype appears in plant material.
4) sample treatment: after about 4 weeks, wilting phenotype appears in responsive type material, and all material is taken out from small flower, takes respectively acrial part fresh weight, is then placed in these materials 85 ℃ of baking ovens dry 48 hours, then weighs material dry weight.
5) Na
+, K
+determination of ion content: the samples weighing after drying is placed on to (HNO in the digestive juice of 10 times of volumes of sample
3: HClO
4=4:1, volume ratio, HNO
3and HClO
4be top grade pure) in predigestion 12 hours, the beaker that digest is housed is placed in to muffle furnace, 175 ℃ of heating 2-3 hour, until that solution becomes is as clear as crystal.In above solution, add deionized water, and transfer in test tube and be settled to 25mL, mix rear absorption 1mL and be diluted to 15mL by deionized water, after mixing, get again 1mL and be diluted to 10mL.Sample is placed in to Atomic Absorption Spectrometer, measures respectively Na in solution
+, K
+ion concentration.
6) result is calculated: experiment adopts randomized complete block design, respectively by acrial part fresh weight, acrial part dry weight, K
+/ Na
+value, as statistical indicator, utilizes SPSS16.0 software to carry out variance analysis.Utilize duncan's new multiple range method to carry out multiple ratio, the horizontal p=0.05 of the significance of difference.
Salt tolerance of corn authentication method provided by the invention, experimental period is short, from starting to process investigation, finishes, and one-month period can complete, and has avoided long problem of field test cycle.By comparing different materials acrial part fresh weight, acrial part dry weight, K after salt stress is processed
+/ Na
+ion concentration ratio, the salt resistance of evaluating material.The method is simple to operate, and testing index only need weigh material weight and ion concentration is measured, and is suitable for extensive corn material and identifies.In addition, the method adopts acrial part fresh weight and dry weight and K
+/ Na
+ion concentration is compared to identification of indicator and acrial part is dry, fresh weight index is verified mutually, has avoided manual operation error, has accurate, reliable feature.
Accompanying drawing explanation
Fig. 1 is that in the embodiment of the present invention 1, different N aCl concentration is processed rear corn phenotype; Wherein, process after 9 corn materials respectively with 0mM, 100mM, 200mM NaCl, CZ-7 shows obvious salt resistance, and B73 shows the responsive phenotype of salt.
Fig. 2 is that in the embodiment of the present invention 2, salt stress affects above-ground plant parts fresh weight; Wherein, abscissa represents different inbred line materials, and ordinate is acrial part fresh weight, the contrast that Control representative is normally watered, seedling acrial part fresh weight after 100mM NaCl represents to process with 100mM NaCl, seedling acrial part fresh weight after 200mM NaCl represents to process with 200mM NaCl; Difference is not remarkable under normal operation for different inbred line materials, and after 100mM and 200mM NaCl processing, acrial part fresh weight significant difference, CZ-7 shows obvious salt resistance, and B73 shows the responsive phenotype of salt.
Fig. 3 is that in the embodiment of the present invention 2, salt stress affects above-ground plant parts dry weight; Wherein, abscissa represents different inbred line materials, and ordinate is acrial part dry weight, the contrast that Control representative is normally watered, seedling acrial part dry weight after 100mM NaCl represents to process with 100mM NaCl, seedling acrial part dry weight after 200mM NaCl represents to process with 200mM NaCl; Difference is not remarkable under normal operation for different inbred line materials, and after NaCl processes, acrial part dry weight significant difference, CZ-7 shows obvious salt resistance, and B73 shows the responsive phenotype of salt.
Fig. 4 is that in the embodiment of the present invention 3, salt stress is processed rear corn material K
+/ Na
+ion concentration ratio; Wherein, abscissa represents different inbred line materials, and ordinate is K
+/ Na
+ion concentration ratio, the contrast that Control representative is normally watered, seedling acrial part K after 100mM NaCl represents to process with 100mM NaCl
+/ Na
+ion concentration ratio, seedling acrial part K after 200mM NaCl represents to process with 200mM NaCl
+/ Na
+ion concentration ratio; Difference is not remarkable under normal operation for different inbred line materials, and after NaCl processes, acrial part dry weight significant difference, CZ-7 shows obvious salt resistance, and B73 shows the responsive phenotype of salt.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.If do not specialize, the conventional means that in embodiment, technological means used is well known to those skilled in the art, the raw materials used commercial goods that is.
The cultivation of embodiment 1 corn seedling and salt stress are processed
1, material is prepared: 9 Seed of maize inbreds of seed of collecting different corn materials, wherein CZ-1, CZ-7, CZ-10, BM-7, Bornali, Shuvra, Mohor, Khoi Bhutta come from Bangladesh Agricultural Research Institute, and B73You Institute of Crop Science, Chinese Academy of Agricultural Science provides.
2, seedling sprouts: by deionized water rinsing 3 times of above-mentioned seed, be placed on germination paper, in 28 ℃ of incubators, sprout 48 hours, each material is done 3 processing, and each processing comprises 3 repetitions, and each repeats to plant 4 strains.
3, condition of culture: be transplanted in the small flower that vermiculite is housed sprouting consistent seed, treat that whole seedling sowings are complete, flowerpot is placed in incubator, condition of culture is: 25 ℃ of illumination 16 hours, intensity of illumination is 450 μ molm-2s-1,23 ℃ of dark 8 hours, relative moisture 65%.Before two leaf stage, seedling is watered the Hoagland nutrient solution (Hoagland & Arnon, 1950) of 1/2 concentration.
4, Stress treatment: after two leaf stage, seedling is divided into 3 processing, the Hoagland nutrient solution of the normal pouring of contrast 1/2 concentration, process the NaCl solution that seedling is watered respectively 100mM and 200mM, the next day, waters once, until wilting phenotype appears in plant material, experiment finishes (Fig. 1).
Embodiment 2 acrial part fresh weights and dry weight weigh
1, acrial part fresh weight weighs: about about 4 weeks of seedling Stress treatment, wilting phenotype appears in part material seedling, and seedling is taken out from flowerpot, along root, cuts acrial part, and quick weigh, is acrial part fresh weight.
2, acrial part fresh weight weighs: will claim the seedling of fresh weight to be placed in paper bag, be placed in 85 ℃ of baking ovens, continued to dry 48 hours, and then weighed dry matter weight, i.e. acrial part dry weight (Fig. 2 and Fig. 3).
Na in embodiment 3 samples
+, K
+determination of ion content
1, sample weighing: after the sample of oven dry is weighed on balance, put into volumetric flask.
2, treatments of the sample: to adding (HNO in the digestive juice of 10 times of volumes of sample in above sample
3: HClO
4=4:1, volume ratio), predigestion 12 hours, will be equipped with digest beaker and be placed in muffle furnace, and 175 ℃ of heating 2-3 hour, until that solution becomes is as clear as crystal.
3, Sample Dilution: add deionized water in digestive juice, and transfer in test tube and be settled to 25mL, mix rear absorption 1mL and be diluted to 15mL by deionized water, get again 1mL after mixing and be diluted to 10mL.
4, Na
+, K
+ion measurement: the solution after dilution is placed in to Atomic Absorption Spectrometer, measures respectively Na
+, K
+ion concentration, using do not add sample digestive juice as blank.
Na
+, K
+ion concentration calculates: by variable concentrations Na
+, K
+the light absorption value of titer, production standard curve, according to calibration curve, calculates light absorption value and ion concentration equation, brings sample light absorption value into equation and obtains Na in sample
+, K
+the content of ion (Fig. 4).
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (2)
1. salt tolerance of corn authentication method, is characterized in that, comprises the following steps:
1) corn seed is germinateed on germination paper, choose and sprout consistent seedling;
2) seedling replanting is entered to be equipped with in the flowerpot of vermiculite, before two leaf stage, water the Hoagland nutrient solution of 1/2 concentration;
3) seedling is after two leaf stage, and control group plant continues the Hoagland nutrient solution of pouring 1/2 concentration, and processed group plant is watered respectively the NaCl salting liquid of 100mM and 200mM, and the next day waters once, until wilting phenotype appears in plant material;
4) plant occurs after wilting phenotype, getting seedling acrial part weighs, be placed in again the dry 36-48 hour of 85-160 ℃ of baking oven, again weigh, the digestive juice that the material of oven dry is placed in to 10 times of volumes of material is processed 8-12 hour, then digestive juice is placed in to muffle furnace in 175-185 ℃ of heating 2-3 hour, until that solution becomes is as clear as crystal, by after above solution dilution, on Atomic Absorption Spectrometer, measure Na
+, K
+ion concentration;
Step 4) digestive juice described in is HNO
3and HClO
4press 4:1 volume ratio mixed preparing.
2. method according to claim 1, is characterized in that step 2) and 3) condition of culture be: 25 ℃ of illumination 16 hours, intensity of illumination is 450 μ molm
-2s
-1, 23 ℃ of dark 8 hours, relative moisture 65%.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255119A (en) * | 2014-09-29 | 2015-01-07 | 甘肃农业大学 | Application method of feeding of different nutrient elements in improving salt tolerance of corns |
| CN105693410A (en) * | 2016-04-10 | 2016-06-22 | 山东省农业科学院玉米研究所 | Nutrient solution for relieving salt stress influences on maize seedlings and application of nutrient solution |
| CN105830724A (en) * | 2016-04-20 | 2016-08-10 | 运城学院 | Method for screening salt-resistant maize variety |
| CN105900636A (en) * | 2016-04-20 | 2016-08-31 | 运城学院 | Method for screening salt-tolerance kidney bean variety |
| CN106416658A (en) * | 2016-08-21 | 2017-02-22 | 山东省农业科学院玉米研究所 | Method and nutrient solution for improving salt tolerance of salt-tolerant corn at seedling stages and application of nutrient solution |
| CN106416773A (en) * | 2016-10-09 | 2017-02-22 | 山东省农业科学院玉米研究所 | Screening and identification method of salt tolerance of corns at seedling stage |
| CN106879445A (en) * | 2017-04-06 | 2017-06-23 | 山东省农业科学院作物研究所 | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material |
| CN107889742A (en) * | 2017-11-24 | 2018-04-10 | 四川农业大学 | The method that haloduric corn kind is cultivated using corn allopolyploid |
| CN108812269A (en) * | 2018-06-13 | 2018-11-16 | 桂林理工大学 | A kind of corn ciltivating process |
| CN108901802A (en) * | 2018-06-13 | 2018-11-30 | 桂林理工大学 | Improve the equipment of corn water planting |
| CN108966729A (en) * | 2018-06-28 | 2018-12-11 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of characterization and evaluation method of Maize at Seedling Stage salt tolerance |
| CN109122254A (en) * | 2018-07-19 | 2019-01-04 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted |
| CN109187863A (en) * | 2018-09-03 | 2019-01-11 | 河北省农林科学院滨海农业研究所 | A kind of greening seedling Seedling Salt-tolerance identification method |
| CN110731223A (en) * | 2019-11-15 | 2020-01-31 | 南京林业大学 | Method for promoting growth of salix jinguiensis under stress of salts |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032222A (en) * | 2007-04-25 | 2007-09-12 | 中国科学院植物研究所 | Method of improving salt resistance of plants through the inducement of gamma-aminobutyric acid |
| CN101785383A (en) * | 2010-03-17 | 2010-07-28 | 吉林省农业科学院 | Method for screening corn salt/alkali-tolerance germplasm |
| CN102524054A (en) * | 2011-12-20 | 2012-07-04 | 东北农业大学 | Method for screening salt-tolerant genetically modified corn |
| CN102577798A (en) * | 2012-02-17 | 2012-07-18 | 河南省农业科学院 | Method for identifying salt tolerance of corn |
| BRPI0819792A2 (en) * | 2008-10-08 | 2012-12-18 | Embrapa | citrus seedling certification equipment, method and system |
| CN103238396A (en) * | 2013-05-28 | 2013-08-14 | 甘肃农业大学 | Screening method of salt resisting germplasms of corn |
| CN103430784A (en) * | 2013-08-23 | 2013-12-11 | 江苏沿江地区农业科学研究所 | Simple method for evaluating salt tolerance of crops at seedling stage |
-
2014
- 2014-04-30 CN CN201410183121.4A patent/CN104012267A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101032222A (en) * | 2007-04-25 | 2007-09-12 | 中国科学院植物研究所 | Method of improving salt resistance of plants through the inducement of gamma-aminobutyric acid |
| BRPI0819792A2 (en) * | 2008-10-08 | 2012-12-18 | Embrapa | citrus seedling certification equipment, method and system |
| CN101785383A (en) * | 2010-03-17 | 2010-07-28 | 吉林省农业科学院 | Method for screening corn salt/alkali-tolerance germplasm |
| CN102524054A (en) * | 2011-12-20 | 2012-07-04 | 东北农业大学 | Method for screening salt-tolerant genetically modified corn |
| CN102577798A (en) * | 2012-02-17 | 2012-07-18 | 河南省农业科学院 | Method for identifying salt tolerance of corn |
| CN103238396A (en) * | 2013-05-28 | 2013-08-14 | 甘肃农业大学 | Screening method of salt resisting germplasms of corn |
| CN103430784A (en) * | 2013-08-23 | 2013-12-11 | 江苏沿江地区农业科学研究所 | Simple method for evaluating salt tolerance of crops at seedling stage |
Non-Patent Citations (4)
| Title |
|---|
| 中国人民共和国林业部科技司: "《林业标准汇编（三）》", 30 June 1991, 中国林业出版社 * |
| 付艳等: "玉米种质苗期耐盐性的评价", 《玉米科学》 * |
| 王玉凤等: "NaCl胁迫对玉米幼苗不同器官离子含量的影响", 《生态学杂志》 * |
| 罗庆云等: "大豆苗期耐盐性鉴定指标的检验", 《大豆科学》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255119A (en) * | 2014-09-29 | 2015-01-07 | 甘肃农业大学 | Application method of feeding of different nutrient elements in improving salt tolerance of corns |
| CN105693410A (en) * | 2016-04-10 | 2016-06-22 | 山东省农业科学院玉米研究所 | Nutrient solution for relieving salt stress influences on maize seedlings and application of nutrient solution |
| CN105830724A (en) * | 2016-04-20 | 2016-08-10 | 运城学院 | Method for screening salt-resistant maize variety |
| CN105900636A (en) * | 2016-04-20 | 2016-08-31 | 运城学院 | Method for screening salt-tolerance kidney bean variety |
| CN106416658A (en) * | 2016-08-21 | 2017-02-22 | 山东省农业科学院玉米研究所 | Method and nutrient solution for improving salt tolerance of salt-tolerant corn at seedling stages and application of nutrient solution |
| CN106416773B (en) * | 2016-10-09 | 2019-08-20 | 山东省农业科学院玉米研究所 | A kind of process for screening and identifying of Maize Seedling salt tolerance |
| CN106416773A (en) * | 2016-10-09 | 2017-02-22 | 山东省农业科学院玉米研究所 | Screening and identification method of salt tolerance of corns at seedling stage |
| CN106879445A (en) * | 2017-04-06 | 2017-06-23 | 山东省农业科学院作物研究所 | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material |
| CN106879445B (en) * | 2017-04-06 | 2019-11-22 | 山东省农业科学院作物研究所 | A kind of method of Rapid identification and screening sorghum germination period salt tolerance material |
| CN107889742A (en) * | 2017-11-24 | 2018-04-10 | 四川农业大学 | The method that haloduric corn kind is cultivated using corn allopolyploid |
| CN108812269A (en) * | 2018-06-13 | 2018-11-16 | 桂林理工大学 | A kind of corn ciltivating process |
| CN108901802A (en) * | 2018-06-13 | 2018-11-30 | 桂林理工大学 | Improve the equipment of corn water planting |
| CN108966729A (en) * | 2018-06-28 | 2018-12-11 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of characterization and evaluation method of Maize at Seedling Stage salt tolerance |
| CN109122254A (en) * | 2018-07-19 | 2019-01-04 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of haloduric corn germplasm quickly, efficiently, identification method can be transplanted |
| CN109187863A (en) * | 2018-09-03 | 2019-01-11 | 河北省农林科学院滨海农业研究所 | A kind of greening seedling Seedling Salt-tolerance identification method |
| CN110731223A (en) * | 2019-11-15 | 2020-01-31 | 南京林业大学 | Method for promoting growth of salix jinguiensis under stress of salts |
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