CN103995080B - The method for building up of a kind of Aconitum teucostomum and processed product HPLC finger-print thereof and finger-print thereof - Google Patents
The method for building up of a kind of Aconitum teucostomum and processed product HPLC finger-print thereof and finger-print thereof Download PDFInfo
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Abstract
The invention discloses the method for building up of a kind of Aconitum teucostomum and processed product HPLC finger-print thereof, comprise the making of the preparation of need testing solution, the preparation of reference substance solution and finger-print.The method for building up of Aconitum teucostomum of the present invention and processed product HPLC finger-print thereof effectively can distinguish various former alkali composition and content, provides quality control and the distinguishing method between true and false of Aconitum teucostomum and processed product thereof accurately---the method for building up of HPLC finger-print.Further, the method for building up stability of HPLC finger-print of the present invention is strong, and the recovery is high, is more suitable for quality control and the True-false distinguish of giving birth to product and processed product thereof for Aconitum teucostomum compared with other method.
Description
Technical field
The present invention relates to the method for building up of a kind of Aconitum teucostomum and processed product HPLC finger-print thereof.
Background technology
Along with the international and domestic research to Chinese medicine proposes " internationalization " " standardization " " scientific " principle of Chinese medicine gradually.Rest in compound preparation level more than the research of rhizome of Chinese monkshood preparation, its effective active composition indefinite just cannot study its concrete mechanism of action further, and this is also the important bottleneck hindering Chinese medicine to go to the world to win world's accreditation.
In aconitum plant, main chemical compositions is diterpene alkaloid, it is many rings nitrogen-containing compound of a class formation complexity, because it has significant physiologically active and larger toxicity, become the focus that people extensively study, but research also focuses mostly on the monkshood radix aconiti agrestis having standards of pharmacopoeia, to the research of Xinjiang Aconitum chemical composition still not deeply, Aconitum teucostomum as diester-type alkaloids constituent class in toxic component contained by Aconitum medicinal plant and monkshood Radix Aconiti Kusnezoffii seemingly.Aconitum teucostomum is poisonous medicinal plant, can must use after concocting attenuation, although adopted certain concocting method attenuation in (as Kazak ethnic population) among the people, the concocting method of Aconitum teucostomum and the quality control of processed product product thereof have still needed to be studied.
Extracting method report at present about Aconitum Alkaloids in Plants has a lot, and extraction efficiency is also uneven, and does not see relevant elaborate report to measuring alkaloidal test sample pre-treating method in Aconitum teucostomum and processed product thereof by HPLC method.
Summary of the invention
The technical problem to be solved in the present invention overcomes existing defect, provides quality control and distinguishing method between true and false--the method for building up of HPLC finger-print of a kind of new Aconitum teucostomum and processed product thereof;
Another object of the present invention is to provide and a kind ofly can be used for the quality control of Aconitum teucostomum and processed product thereof and the finger-print of True-false distinguish.
Object of the present invention carrys out specific implementation by the following technical programs:
A method for building up for Aconitum teucostomum and processed product HPLC finger-print thereof, comprises the making of the preparation of need testing solution, the preparation of reference substance solution and finger-print.
The preparation method of described test sample is:
A. getting Aconitum teucostomum sample powder adds in ammoniacal liquor after airtight infiltration, and with 95% alcohol solvent, after adopting ultrasonic method to extract, weighed weight is also supplied with 95% ethanol again, and filter, filtrate less than 60 DEG C volatilizes;
B. residue adds watery hydrochloric acid and regulates PH about 1, then adds ammoniacal liquor and adjust pH about 10, then uses chloroform extraction 3 times, merge methenyl choloride layer and less than 60 DEG C volatilize, residue adds in acetonitrile, and miillpore filter filters, and gets subsequent filtrate and get final product.
Preferably, in described step a, described Aconitum teucostomum sample powder: ammoniacal liquor: 95% ethanol is 5g:5mL:50mL.
Preferably, in described step a, when described ultrasonic method extracts, Extracting temperature is 23 DEG C, ultrasound intensity is 53KHz, extract 30min.
Preferably, in described step b, described watery hydrochloric acid is 1% hydrochloric acid solution.
The preparation method of described reference substance is:
Get Lappaconitine, aconitine, mesaconine, benzoyl aconine, benzoylmesaconine, benzoyl time aconine respectively, be mixed with chromatogram acetonitrile constant volume the mixing reference substance solution that concentration is respectively 4.1mg/mL, 2mg/mL, 2.1mg/mL, 0.3mg/mL, 0.4mg/mL, 0.2mg/mL.
The method for making of described finger-print is: adopt high effective liquid chromatography for measuring to analyze need testing solution, wherein, chromatographic condition is:
Chromatographic column: XBridgeTM-C18,250mm × 4.6mm, 5 μm of chromatographic columns;
Filling agent: octadecylsilane chemically bonded silica;
Mobile phase: acetonitrile (B)-0.05mol/L Ammoniom-Acetate (D);
Elution program: adopt gradient elution;
Determined wavelength:: 235nm;
Flow velocity: 1.0ml/min;
Column temperature: 30 DEG C
Sample size: 10 μ L
Preferably, the PH of the Ammoniom-Acetate in described mobile phase is 7.25, and the program of described gradient elution is 0min → 155min → 158min → 160min, acetonitrile 18% → 31% → 35% → 45%.
A kind of Aconitum teucostomum and processed product HPLC finger-print thereof (are corresponding in turn to 1 benzoyl aconine see the 1st, 2,3,4,5, No. 6 chromatographic peak in accompanying drawing 1, figure; 2 benzoyls time aconine; 3 Lappaconitines; 4 unknown compounds 1; 5 mesaconines; 6 aconitines, wherein No. 3 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 34.1min;
No. 2 peaks, Average residence time t is 40.7min;
No. 3 peaks, Average residence time t is 50.4min;
No. 4 peaks, Average residence time t is 80.8min;
No. 5 peaks, Average residence time t is 90.0min;
No. 6 peaks, Average residence time t is 108.6min;
Aconitum teucostomum 3 kinds of processed products (CP method, literature method, Kazak's method) all have 6 characteristic peaks, and the 1st, 2,3,4,5, No. 6 chromatographic peak is corresponding in turn to 1 benzoylmesaconine; 2 benzoyl aconines; 3 benzoyls time aconine; 4 Lappaconitines; 5 unknown compounds 1; 6 mesaconines, wherein No. 4 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 27.4min;
No. 2 peaks, Average residence time t is 34.1min;
No. 3 peaks, Average residence time t is 40.7min;
No. 4 peaks, Average residence time t is 50.4min;
No. 5 peaks, Average residence time t is 80.8min;
No. 6 peaks, Average residence time t is 90.0min;
In order to further illustrate essence of the present invention, inventor has carried out Precision Experiment, reappearance experiment, stability experiment and application of sample recovery experiment for the technology of the present invention and has verified.
One, Precision Experiment
Get above-mentioned mixing reference substance solution according to above-mentioned chromatographic condition continuous sample introduction 6 times, the RSD calculating the content of 3 kinds of compounds is respectively 2.24%, 1.56%, 2.25%, and result display instrument precision is higher.
Two, reappearance experiment
Precision takes Aconitum teucostomum medicinal material 6 parts, prepare need testing solution as stated above, by above-mentioned chromatographic condition sample introduction, measure the peak area of Lappaconitine, aconitine, mesaconine, the average content of calculating Lappaconitine is 2.7535mg/g, RSD is 2.06% (n=6); The average content of aconitine is 0.0686mg/g, RSD is 1.16% (n=6); The average content of mesaconine is 0.3713mg/g, RSD is 2.57% (n=6), and result shows that the repeatability of this method is better.
Three, stability experiment
Get Aconitum teucostomum medicinal material and be about 5g, accurately weighed, make need testing solution as stated above, by above-mentioned chromatographic condition respectively 0,4,8,14,20,24h sample introduction 10 μ L, record chromatographic peak area, the RSD of calculating Lappaconitine, aconitine, mesaconine peak area is respectively 2.77%, 1.61%, 2.08%, result shows that need testing solution is good at 24h internal stability.
Four, application of sample recovery experiment
Getting Aconitum teucostomum medicinal powder, to be about 5g accurately weighed, make need testing solution as stated above, add the Lappaconitine of 3 kinds of variable concentrations, aconitine, mesaconine, benzoylmesaconine, benzoyl aconine, mixing reference substance respectively, measure by above-mentioned chromatographic condition sample introduction, calculate average recovery, the results are shown in Table 1-6.
Table 1 Lappaconitine average recovery measurement result
Table 2 aconitine average recovery measurement result
Table 3 mesaconine average recovery measurement result
Table 4 benzoyl mesaconine average recovery measurement result
Table 5 benzoyl aconine average recovery measurement result
Table 6 benzoyl Hypaconitine average recovery measurement result
Five, assay
Get each Aconitum teucostomum medicinal material and be about 5g, by above-mentioned chromatographic condition replicate determination 3 times, calculate the content of Lappaconitine in Aconitum teucostomum medicinal material, aconitine, mesaconine, benzoylmesaconine, benzoyl aconine, benzoyl time aconine according to chromatographic peak area, the results are shown in Table 7.
Alkaloid component assay result in table 7 Aconitum teucostomum and processed product thereof
In assay result, in each processed product, the relatively raw product of monoester alkaloid are higher, and the relatively raw product of the diester-type alkaloids composition that toxicity is larger greatly reduce, and show that the diester-type alkaloids composition of Aconitum teucostomum toxicity after concocting is in reduction; The simultaneously content of Lappaconitine, aconitine, mesaconine, benzoylmesaconine, benzoyl aconine and benzoyl time aconine in high-efficient liquid phase gradient elution chromatography Simultaneously test Aconitum teucostomum, method is feasible, easy and simple to handle, can be used for mensuration and the quality control of active component in Aconitum teucostomum, is also that the utilization of resources and the quality standard formulation of Aconitum teucostomum is laid a good foundation.
Beneficial effect of the present invention:
(1) preparation method of need testing solution is easy, and be easy to operation, the test period is short, and the need testing solution impurity of preparation is few, more totally, not easily pollutes chromatographic column, and the chromatogram impurity signal interference obtained is few;
(2) chromatographic condition easily realizes, acetonitrile system post pressure is lower, be conducive to the stable of system, and chromatographic column selects water-fast XBridgeTM-C18 filler, prevents common C18 filler chromatographic column from using water as and causes the instability of chromatographic system for column cap during mobile phase subsides;
(3) after adopting chlorine water to infiltrate 1h, use 95% ethanol as extraction agent again, in conjunction with ultrasonic extracting method, be more suitable for the extraction of Aconitum teucostomum effective constituent, can Small molecular effective component at utmost in extracting and developing Aconitum teucostomum, as salt bromic acid lappaconitine etc., and set up the fingerprint spectrum method of Aconitum teucostomum on this basis, make it have more specific aim and practicality to the quality control of Aconitum teucostomum;
(4) each Characteristic chromatographic peak under chromatographic condition of the present invention all achieves baseline separation, fuzzy diagnosis can not only be carried out to Aconitum teucostomum on the whole, also can contrast object of reference chromatogram carries out clear discriminating and Aconitum teucostomum polycomponent quantitative analysis to Aconitum teucostomum;
(5) the inventive method stability is high, favorable reproducibility, characteristic peak are many, the quality of Aconitum teucostomum medicinal material (comprising processed product) can be evaluated comprehensively, exactly, be suitable for the identify and control to Aconitum teucostomum and the processed product true and false thereof, the place of production and quality.
From above-mentioned experiment, the method for building up of Aconitum teucostomum of the present invention and processed product HPLC finger-print thereof effectively can distinguish various former alkali composition and content, provides quality control and the distinguishing method between true and false of Aconitum teucostomum and processed product thereof accurately--the method for building up of HPLC finger-print.Further, the method for building up stability of HPLC finger-print of the present invention is strong, and the recovery is high, and comparatively other method is more suitable for for quality control and True-false distinguish.Finger-print of the present invention is utilized to carry out quality control and True-false distinguish to Aconitum teucostomum and processed product thereof, simple to operate, meanwhile, be also that the utilization of resources and the quality standard formulation of Aconitum teucostomum is laid a good foundation.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for instructions, together with embodiments of the present invention for explaining the present invention, is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the HPLC finger-print of each known compound standard items in Aconitum teucostomum and processed product thereof in the present invention;
Fig. 2 is the raw product HPLC-FPS of Aconitum teucostomum of the present invention;
Fig. 3 is Aconitum teucostomum CP method processed product HPLC finger-print of the present invention;
Fig. 4 is Aconitum teucostomum literature method processed product HPLC finger-print of the present invention;
Fig. 5 is Aconitum teucostomum Sa Croat method processed product HPLC finger-print of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
Embodiment 1:
The method for building up of Aconitum teucostomum and processed product HPLC finger-print thereof:
Aconitum teucostomum medicinal material totally 10 batches, is all collected in Altay, Xinjiang and Ili Prefecture; Use Waters2695 high performance liquid chromatograph (Waters, US, quaternary pump, online degasser, PDA detecting device); Reagent is chromatographically pure or analyzes pure.
1) preparation of reference substance solution
Get Lappaconitine, aconitine, mesaconine, benzoyl aconine, benzoylmesaconine, the benzoyl time appropriate reference substance of aconine accurately weighed respectively, be mixed with concentration with chromatogram acetonitrile constant volume and be respectively 4.1mg/mL, 2.0mg/mL, 2.1mg/mL, 0.3mg/mL, the mixing reference substance solution of 0.4mg/mL, 0.2mg/mL.
2) preparation of need testing solution
Get Aconitum teucostomum sample powder (cross No. three sieves) about 5g and accurately weighed, the weighed quality of 50mL95% ethanol is added after adding the airtight infiltration of ammoniacal liquor 5mL, ultrasonic (23 DEG C, weighed weight supplying with absolute ethyl alcohol again after 53KHz) extracting 30min, filter, filtrate less than 60 DEG C volatilizes; Residue adds 20mL1%HCL and adjusts PH about 1, then adds ammoniacal liquor tune pH about 10, then uses chloroform extraction 3 times (each 10mL), merge methenyl choloride layer and less than 60 DEG C volatilize, residue adds acetonitrile and is settled to 5ml volumetric flask, and miillpore filter filters, and gets subsequent filtrate and get final product.
3) making of finger-print
Adopt high effective liquid chromatography for measuring to analyze need testing solution, wherein, chromatographic condition is:
Chromatographic column: XBridgeTM-C18 (250mm × 4.6mm, 5 μm) chromatographic column; Filling agent: octadecylsilane chemically bonded silica; Mobile phase: acetonitrile (B)-0.05mol/L Ammoniom-Acetate (adjusting pH to be 7.25) (D) gradient elution. (elution program is in table 8); Determined wavelength: 235nm; Flow velocity: 1.0ml/min; Column temperature: 30 DEG C; Sample size: 10 μ L.
Table 8 gradient elution program
Embodiment 2:
Aconitum teucostomum raw product HPLC finger-print
Aconitum teucostomum medicinal material totally 10 batches, is all collected in Altay, Xinjiang and Ili Prefecture.
Each batch of Aconitum teucostomum finger-print is obtained by method described in the embodiment of the present invention 1, by the comparison of 10 batches of HPLC collection of illustrative plates, carry out similarity evaluation, determine 6 common characteristic peaks (see accompanying drawing 2, its No. 1 to 6, common characteristic peak has from left to right been marked respectively in figure), in figure, the 1st, 2,3,4,5, No. 6 chromatographic peak is corresponding in turn to 1 benzoylmesaconine; 2 benzoyls time aconine; 3 Lappaconitines; 4 unknown compounds 1; 5 mesaconines; 6 aconitines, wherein No. 3 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 27.4min;
No. 2 peaks, Average residence time t is 40.7min;
No. 3 peaks, Average residence time t is 50.4min;
No. 4 peaks, Average residence time t is 80.8min;
No. 5 peaks, Average residence time t is 90.0min;
No. 6 peaks, Average residence time t is 108.6min.
Embodiment 3:
Aconitum teucostomum CP method processed product HPLC finger-print
Aconitum teucostomum CP method processed product: get Aconitum teucostomum, separately, the 6h that is soaked in water, to interior without the dry heart, take out, adds water boil 2h for size, get large cut in without the white heart, mouth taste micro-have a numb feeling in the tongue time, take out, 70 DEG C of low temperature dryings, pulverized No. three sieves, to obtain final product.
According to the method establishment finger-print in embodiment 1, gained Aconitum teucostomum processed product (CP method) HPLC finger-print is (see accompanying drawing 3, its No. 1 to 6, common characteristic peak has from left to right been marked respectively) in figure, have 6 characteristic peaks, the 1st, 2,3,4,5, No. 6 chromatographic peak is corresponding in turn to 1 benzoylmesaconine; 2 benzoyl aconines; 3 benzoyls time aconine; 4 Lappaconitines; 5 unknown compounds 1; 6 mesaconines, wherein No. 4 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 27.4min;
No. 2 peaks, Average residence time t is 34.1min;
No. 3 peaks, Average residence time t is 40.7min;
No. 4 peaks, Average residence time t is 50.4min;
No. 5 peaks, Average residence time t is 80.8min;
No. 6 peaks, Average residence time t is 90.0min.
Embodiment 4:
Aconitum teucostomum literature method processed product HPLC finger-print
Aconitum teucostomum literature method processed product: after Aconitum teucostomum cleaning, vexed profit, fixed temperature is 126 DEG C, and pressure is 0.15MPa, steams 90min, 70 DEG C of low temperature dryings, pulverizes No. three sieves, and to obtain final product.
According to the method establishment finger-print in embodiment 1, the HPLC finger-print of gained Aconitum teucostomum processed product (literature method) is (see accompanying drawing 4, its No. 1 to 6, common characteristic peak has from left to right been marked respectively in figure): have 6 characteristic peaks, the 1st, 2,3,4,5, No. 6 chromatographic peak is corresponding in turn to 1 benzoylmesaconine; 2 benzoyl aconines; 3 benzoyls time aconine; 4 Lappaconitines; 5 unknown compounds 1; 6 mesaconines, wherein No. 4 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 27.4min;
No. 2 peaks, Average residence time t is 34.1min;
No. 3 peaks, Average residence time t is 40.7min;
No. 4 peaks, Average residence time t is 50.4min;
No. 5 peaks, Average residence time t is 80.8min;
No. 6 peaks, Average residence time t is 90.0min.
Embodiment 5:
Aconitum teucostomum Sa Croat method processed product HPLC finger-print
Aconitum teucostomum Kazak method processed product: by Aconitum teucostomum bubble in water, after soaking 6h, then (liquid medicine ratio is boil 1h altogether after 8% to get 8% succus liquiritiae, filter) and 10% decoction of black soybean (liquid medicine ratio is boil 1h altogether after 10%, filter) mix thoroughly with Aconitum teucostomum and jointly boil 2h and taste without fiber crops without the white heart, mouth to interior or micro-ly have numb feeling in the tongue degree of being, 70 DEG C of low temperature dryings, pulverized No. three sieves, to obtain final product.
Set up finger-print according to the method described above, the HPLC finger-print of gained Aconitum teucostomum 3 kinds of processed products (Kazak's method) is (see accompanying drawing 5, its No. 1 to 6, common characteristic peak has from left to right been marked respectively in figure): have 6 characteristic peaks, the 1st, 2,3,4,5, No. 6 chromatographic peak is corresponding in turn to 1 benzoylmesaconine; 2 benzoyl aconines; 3 benzoyls time aconine; 4 Lappaconitines; 5 unknown compounds 1; 6 mesaconines, wherein No. 4 peaks are the strongest, and collection of illustrative plates total length is 160min, and Average residence time t is as follows:
No. 1 peak, Average residence time t is 27.4min;
No. 2 peaks, Average residence time t is 34.1min;
No. 3 peaks, Average residence time t is 40.7min;
No. 4 peaks, Average residence time t is 50.4min;
No. 5 peaks, Average residence time t is 80.8min;
No. 6 peaks, Average residence time t is 90.0min.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. a method for building up for Aconitum teucostomum and processed product HPLC finger-print thereof, is characterized in that: the making comprising the preparation of need testing solution, the preparation of reference substance solution and finger-print, and concrete steps are as follows:
1) preparation of need testing solution
A. getting Aconitum teucostomum sample powder adds in ammoniacal liquor after airtight infiltration, with 95% alcohol solvent, after adopting ultrasonic method to extract, and weighed weight supplying with absolute ethyl alcohol again, filter, filtrate less than 60 DEG C volatilizes;
B. residue adds watery hydrochloric acid and regulates pH about 1, then adds ammoniacal liquor and adjust pH about 10, then uses chloroform extraction 3 times, merge methenyl choloride layer and less than 60 DEG C volatilize, residue adds in acetonitrile, and miillpore filter filters, and gets subsequent filtrate and get final product;
2) preparation of reference substance solution
Get Lappaconitine, aconitine, mesaconine, benzoyl aconine, benzoylmesaconine, benzoyl time aconine respectively, be mixed with chromatogram acetonitrile constant volume the mixing reference substance solution that concentration is respectively 4.1mg/mL, 2.0mg/mL, 2.1mg/mL, 0.3mg/mL, 0.4mg/mL, 0.2mg/mL;
3) making of finger-print
Adopt high effective liquid chromatography for measuring to analyze need testing solution, wherein, chromatographic condition is:
Chromatographic column: XBridgeTM-C18,250mm × 4.6mm, 5 μm of chromatographic columns;
Filling agent: octadecylsilane chemically bonded silica;
Mobile phase: acetonitrile (B)-0.05mol/L Ammoniom-Acetate (D);
Elution program: adopt gradient elution;
Determined wavelength: 235nm;
Flow velocity: 1.0ml/min;
Column temperature: 30 DEG C;
Sample size: 10 μ L;
In described step 3), the pH of the Ammoniom-Acetate in described mobile phase is 7.25, and the program of described gradient elution is 0min → 155min → 158min → 160min, acetonitrile 18% → 31% → 35% → 45%.
2. the method for building up of Aconitum teucostomum according to claim 1 and processed product HPLC finger-print thereof, is characterized in that: in the step a of described step 1), described Aconitum teucostomum sample powder: ammoniacal liquor: 95% ethanol is 5g:5mL:50mL.
3. the method for building up of Aconitum teucostomum according to claim 1 and processed product HPLC finger-print thereof, is characterized in that: in the step a of described step 1), and when described ultrasonic method extracts, Extracting temperature is 23 DEG C, ultrasound intensity is 53KHz, extract 30min.
4. the method for building up of Aconitum teucostomum according to claim 1 and processed product HPLC finger-print thereof, is characterized in that: in the step b of described step 1), described watery hydrochloric acid is 1% hydrochloric acid solution.
5. an Aconitum teucostomum HPLC-FPS, it is characterized in that: make according to the method for any one of claim 1-4 the standard HPLC finger-print obtaining Aconitum teucostomum medicinal material, there are 6 characteristic peaks, wherein No. 3 peaks are the strongest, collection of illustrative plates total length is 160min, and the Average residence time t of 6 characteristic peaks is as follows:
No. 1 peak, Average residence time t is 27.4min, and corresponding compound is benzoylmesaconine;
No. 2 peaks, Average residence time t is 40.7min, and corresponding compound is benzoyl time aconine;
No. 3 peaks, Average residence time t is 50.4min, and corresponding compound is Lappaconitine;
No. 4 peaks, Average residence time t is 80.8min;
No. 5 peaks, Average residence time t is 90.0min, and corresponding compound is mesaconine;
No. 6 peaks, Average residence time t is 108.6min, and corresponding compound is aconitine.
6. an Aconitum teucostomum processed product HPLC finger-print, it is characterized in that: profit requires that the method for any one of 1-4 makes the HPLC finger-print of the different processed product of Aconitum teucostomum, have 6 characteristic peaks, retention time is respectively 27.4min, 34.1min, 40.7min, 50.4min, 80.8min, 90.0min, and these characteristic peaks form Aconitum teucostomum processed product fingerprint characteristic.
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