CN103993422B - A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt and preparation method thereof - Google Patents
A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of regenerated silk fibroin/chitosan derivatives blended fiber felt and preparation method thereof, in the blended fiber felt, the weight/mass percentage composition of chitosan derivatives is 1～5%, and remaining is regenerated silk fibroin；The blended fiber felt fracture strength is 1.75～2.8MPa, and initial moduluss are 70～280MPa.Preparation method includes：With Bombyx bombycis as raw material, regenerated silk fibroin water solution is prepared；Water-solubility chitosan derivative is soluble in water, obtain chitosan derivatives aqueous solution；Above two solution is mixed with regenerated silk fibroin/chitosan derivatives blending aqueous solution by a certain percentage, then blended fiber felt is prepared using electrostatic spinning technique.Blended fiber felt prepared by the method not only is compared pure regenerated silk fibroin fiber felt and is increased substantially by mechanical property, also there is the performance such as good biocompatibility, moisture absorbability and moisture retentivity, broad-spectrum antibacterial, it is adaptable to weave, the field such as biomedical teleocracy support.
Description
Technical field
The invention belongs to blended fiber felt preparing technical field, is related to a kind of regenerated silk fibroin/chitosan derivatives common
Combined filament felt and preparation method thereof.
Background technology
Fibroin albumen is that oxygen permeability of breathing freely is good, biofacies by natural silk degumming, as a kind of little to human body inflammatory reaction
The good natural material of capacitive, is increasingly paid close attention to by people.Fibroin albumen is dissolved the regenerated silk fibroin for obtaining can be by
It is processed into the material of the various ways such as gel, thin film and fiber felt.Can be required according to materials'use, be changed by post-process treatment
Fibroin material degree of crystallinity, controls its degradation rate and mechanical property again.But pure silk fibroin material mechanical performance is low, deposits
When moisture content is low, fragility becomes big, and the defect such as mechanical strength and degradation in low moisture environments, this seriously limit which and extensively should
With.
Shitosan is to process the product for obtaining by chitin through deacetylated, and in general, chitin N- acetyl group is sloughed
More than 55% can be referred to as shitosan.Chitin is widely present in the shells such as arthropod shrimp, Eriocheir sinensiss, insecticide carapace, and
In Mycophyta cell wall, it is storage is only second to cellulose on the earth biological polyoses.The shitosan and shitosan that process is obtained spreads out
Biological due to have have no toxic side effect, non-immunogenicity, excellent biocompatibility, biodegradability, broad-spectrum antibacterial etc.,
It is used widely in fields such as weaving, biomedicines.Some chitosan derivatives such as hydroxypropyl chitosan, ethoxy shell gather
Sugar, carboxymethyl chitosan, carboxyetbyl chitosan etc. not only remain the original excellent properties of shitosan, but also with good
Water solublity, the machinability for significantly improving.Shitosan and part chitosan derivatives have been spun into fiber, for caning absorb
Suture, eliminates patient and takes out stitches pain；Medical dressing is used as, is suitable for the various wounds for the treatment of；May further be used to processing of weaving cotton cloth
Into various functions product, such as health care underwear, antibacterial deodourizing bedding, antibacterial medical clothing of nurses etc..But pure chitosan and
Its derivant fiber line density is bigger than normal, low strength, processes physics bad mechanical property after weaving, expensive, is processed into
This height.
Yuhui Ruan are equal to " the Preparation of3D Fibroin/Chitosan Blend for delivering for 2011
It is related to 3D fibroin eggs in Porous Scaffold for Tissue Engineering Via a Simplified Method "
The preparation of in vain/chitosan blend porous support, the blending support tensile strength of addition shitosan prepared by this method is more than pure
Silk fibroin bracket is much lower, and porous support drawing is blended stretches intensity less than 0.055MPa；Increase with fibroin albumen content, blending
Support tensile strength declines after first slightly improving, and the blending support tensile strength highest of content 75wt% fibroin albumen is only reached
0.045MPa.Kuihua Zhang are equal to " the Genipin-crosslinked silk fibroin/ for delivering for 2010
hydroxybutyl chitosan nanofibrous scaffolds for tissue-engineering
Fibroin albumen/hydroxyl butyl chitosan nano fiber the support of genipin crosslinking is prepared in application ", changes cross-linking agent
Content and crosslinking time, in document, prepared support ultimate tensile strength is 39.5MPa, and maximum elongation at break is 11%,
But the use of extra cross-linking agent can reduce the biocompatibility that support is blended, and increase preparation process, improve production cost.Open
Number for CN103436985A Chinese invention patents, a kind of preparation method of silk fibroin/chitosan blend nanofiber is disclosed,
With the spinning solution that regenerated silk fibroin formic acid solution is mixed with shitosan trifluoroacetic acid solution, carry out electrostatic spinning and obtain fibroin
Protein/chitosan blending nano-fiber membrane.But the method step is various, not only unfriendly to environment using organic solvent, and
And reduce the biocompatibility of material, and this patent does not mention impact of the shitosan to blending nano-fiber membrane mechanical property.
The Chinese invention patent of Publication No. CN102936794A, discloses a kind of fibroin albumen/carboxymethyl chitosan/polycyclic oxygen second
Alkane/Polyethylene Glycol vitamin E amber claps the preparation method of acid esters/sodium ascorbyl phosphate composite nano-fiber membrane, and the method is adopted
, used as solvent, nontoxic pollution-free, biocompatibility are good for water, but fibrous membrane prepared by the method only has appropriate mechanical property
Can, and this patent does not mention impact of the chitosan derivatives to blending nano-fiber membrane mechanical property.Up to the present, both at home and abroad
Yet there are no with regenerated silk fibroin/chitosan derivatives aqueous solution, prepare what mechanical property was increased substantially by electrostatic spinning
Regenerated silk fibroin/chitosan derivatives blended fiber felt correlational study, document report or patent.
Content of the invention
It is an object of the invention to provide a kind of regenerated silk fibroin/chitosan derivatives blended fiber felt and its preparation side
Method, the present invention are that regenerated silk fibroin is blended with chitosan derivatives, new better than one pack system performance to obtain mechanical property
Fiber type felt.The addition of chitosan derivatives substantially increases the mechanical property of fiber felt, and this is due to chitosan derivatives point
Hydrogen bond can be formed with the amino on fibroin albumen chain containing great amount of hydroxy group in subchain, shitosan first destroys original fibroin albumen point
Hydrogen bond in sub, forms the hydrogen bond between the shitosan and silk fibroin molecular that resets, and the active force of hydrogen bond is more than intermolecular model moral
Hua Liqiang, can improve mechanical property.The mechanism of action that chitosan derivatives improve blended fiber felt mechanical strength from ethanol is different,
Ethanol water post processing, mainly hydrone first increase the free volume of silk fibroin molecular chain, and molecule enters fibre to ethanol again
Promote to form the hydrogen bond between silk fibroin molecular chain between dimension.
A kind of regenerated silk fibroin/chitosan derivatives blended fiber the felt of the present invention, in the blended fiber felt, shell gathers
The weight/mass percentage composition of sugar derivativess is 1～5%, and remaining is regenerated silk fibroin；The blended fiber felt fracture strength is
1.75～2.8MPa, initial moduluss are 70～280MPa, respectively than obtained pure regenerated silk fibroin fiber felt under the same terms
Improve 25～100%, 15～350%.
As preferred technical scheme：
A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt as above, the blended fiber felt is through second
After alcohol-water solution immersion treatment, its fracture strength be 4～7.5MPa, initial moduluss be 360～450MPa, energy to failure be 60～
120J·Kg-1, 30～150%, 100 are improve than the pure regenerated silk fibroin fiber felt through the same terms post processing respectively～
150%th, 50～200%.
A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt as above, described through Ethanol Treatment is
Refer to and blended fiber felt fixed length is soaked in 10～30min in 80～95vol% ethanol waters.
A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt as above, described chitosan derivatives
For the one kind in hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl chitosan, carboxyetbyl chitosan.
A kind of regenerated silk fibroin/chitosan derivatives blended fiber felt as above, the hole of the blended fiber felt
Gap rate is 65～80%, and the scope for constituting the blended fiber diameter of fiber felt is 200nm～2.4 μm.
Present invention also offers a kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, including
Following steps：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
Concentration is in the lithium bromide water solution of 9.0～9.3mol/L；After by this solution centrifugal, filtration, dialysis and concentration, quality hundred is obtained
Divide than being 25～35% regenerated silk fibroin water solutions；
(2) chitosan derivatives are dissolved in deionized water, prepare the chitosan derivative that mass percent is 1～5%
Thing aqueous solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/shell with the chitosan derivatives aqueous solution
Polysaccharid derivative mass ratio is 100:1～5 mixing, prepares regenerated silk fibroin/chitosan derivatives blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/chitosan derivatives prepared by step (3), carries out
Electrostatic spinning, that is, obtain regenerated silk fibroin/chitosan derivatives blended fiber felt.
The preparation method of a kind of regenerated silk fibroin/chitosan derivatives blended fiber felt as above, the step
(4) in, electrospinning conditions are：Spinning voltage is 15～30kV, and spinning flow velocity is 0.1～1.0mL/h, receive distance for 5～
15cm, relative humidity are 45～55%.
The present invention provide a kind of raw material can natural regeneration, the regenerated fibroin of product degradable is with chitosan derivatives altogether
Combined filament felt and preparation method thereof, the method are simple, easy to operate, not using organic solvent in whole process, to environment without
Pollution；Compared with pure regenerated silk fibroin fiber felt, which breaks the regenerated fibroin of gained/chitosan derivatives blended fiber felt
Split energy, fracture strength and initial moduluss to be all greatly improved, also have antibacterial and deodouring, antistatic, breathability strong, good
The excellent properties such as moisture retentive heat insulation.
Beneficial effect：
The present invention is blended with chitosan derivatives using regenerated silk fibroin, on the one hand maintains regenerated silk fibroin biological
The compatibility, on the other hand improves its mechanical property again, and blended fiber felt fracture strength, initial moduluss, energy to failure are significantly carried
Height, additionally so that blended fiber felt has antibacterial action, is suitable for development function health promoting clothes, is more suitably applied to antibacterial
Or the tissue engineering bracket of antibacterial demand, it is to avoid tissue infects；Present invention uses the good chitosan derivative of water solublity
Thing, it is to avoid organic solvent, the such as use of formic acid, trifluoroacetic acid, hexafluoroisopropanol etc., reduces environmental pollution, substantially reduces
Cost, meets Modern Green commercial production theory.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate this
Bright rather than restriction the scope of the present invention.In addition, it is to be understood that after the content for having read instruction of the present invention, art technology
Personnel can be made various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Fixed scope.
Embodiment 1
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.0mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 25% regenerated silk fibroin water solution；
(2) by hydroxypropyl chitosan solution in deionized water, the hydroxypropyl chitosan that mass percent is 1% is prepared water-soluble
Liquid；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/hydroxyl with the hydroxypropyl sugar aqueous solution
Propyl group chitosan mass ratio is 100:1 mixing, prepares regenerated silk fibroin/hydroxypropyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/hydroxypropyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain the derivative blended fiber felt of regenerated silk fibroin/hydroxypropyl chitosan.Electrospinning conditions are：Spinning
Voltage is 15kV, and spinning flow velocity is 0.1mL/h, and it is 5cm to receive distance, and relative humidity is 45%.
Prepared blended fiber felt fracture strength is 2.1MPa, and initial moduluss are 100MPa；The blended fiber felt is fixed
Length is soaked in 80vol% ethanol waters after 10min, and its fracture strength is 5.5MPa, and initial moduluss are 360MPa, energy to failure
For 90J Kg-1；The porosity of the blended fiber felt is 78%, and the scope for constituting the blended fiber diameter of fiber felt is 400nm
～1.8 μm.
Embodiment 2
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.1mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 30% regenerated silk fibroin water solution；
(2) hydroxyethyl chitosan is dissolved in deionized water, prepares the hydroxyethyl chitosan water that mass percent is 2%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/hydroxyl with the hydroxyethyl chitosan aqueous solution
Ethyl chitosan mass ratio is 100:1 mixing, prepares regenerated silk fibroin/hydroxyethyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/hydroxyethyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain the derivative blended fiber felt of regenerated silk fibroin/hydroxyethyl chitosan.Electrospinning conditions are：Spinning
Voltage is 20kV, and spinning flow velocity is 0.3mL/h, and it is 10cm to receive distance, and relative humidity is 50%.
Prepared blended fiber felt fracture strength is 1.75MPa, and initial moduluss are 160MPa.The blended fiber felt is fixed
Length is soaked in 80vol% ethanol waters after 20min, and its fracture strength is 4MPa, and initial moduluss are 380MPa, and energy to failure is
95J·Kg-1；The porosity of the blended fiber felt be 80%, constitute fiber felt blended fiber diameter scope be 200nm～
2μm.
Embodiment 3
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.2mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 33% regenerated silk fibroin water solution；
(2) carboxymethyl chitosan is dissolved in deionized water, prepares the carboxymethyl chitosan sucrose solution that mass percent is 3%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/carboxylic with the carboxymethyl chitosan sugar aqueous solution
Methyl chitosan mass ratio is 100:2 mixing, prepare regenerated silk fibroin/carboxymethyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/carboxymethyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain regenerated silk fibroin/carboxymethyl chitosan blended fiber felt.Electrospinning conditions are：Spinning voltage is
20kV, spinning flow velocity are 0.5mL/h, and it is 15cm to receive distance, and relative humidity is 50%.
Prepared blended fiber felt fracture strength is 2.3MPa, and initial moduluss are 70MPa.The blended fiber felt fixed length
It is soaked in 85vol% ethanol waters after 30min, its fracture strength is 6.4MPa, and initial moduluss are 400MPa, and energy to failure is
105J·Kg-1；The porosity of the blended fiber felt is 75%, and the scope for constituting the blended fiber diameter of fiber felt is 400nm
～2.2 μm.
Embodiment 4
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.3mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 35% regenerated silk fibroin water solution；
(2) carboxyetbyl chitosan is dissolved in deionized water, prepares the carboxyetbyl chitosan water that mass percent is 5%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/carboxylic with the carboxyetbyl chitosan aqueous solution
Ethyl chitosan mass ratio is 100:3 mixing, prepare regenerated silk fibroin/carboxyetbyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/carboxyetbyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain regenerated silk fibroin/carboxyetbyl chitosan blended fiber felt.Electrospinning conditions are：Spinning voltage is
25kV, spinning flow velocity are 1mL/h, and it is 10cm to receive distance, and relative humidity is 50%.
Prepared blended fiber felt fracture strength is 2.5MPa, and initial moduluss are 280MPa.The blended fiber felt is fixed
Length is soaked in 90vol% ethanol waters after 20min, and its fracture strength is 5.9MPa, and initial moduluss are 450MPa, energy to failure
For 110J Kg-1；The porosity of the blended fiber felt is 76%, and the scope for constituting the blended fiber diameter of fiber felt is
400nm～2.4 μm.
Embodiment 5
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.0mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 30% regenerated silk fibroin water solution；
(2) hydroxypropyl chitosan is dissolved in deionized water, prepares the hydroxypropyl sucrose solution that mass percent is 3%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/hydroxyl with the hydroxypropyl sugar aqueous solution
Propyl group chitosan mass ratio is 100:2 mixing, prepare regenerated silk fibroin/hydroxypropyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/hydroxypropyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain regenerated silk fibroin/hydroxypropyl chitosan blended fiber felt.Electrospinning conditions are：Spinning voltage is
25kV, spinning flow velocity are 1mL/h, and it is 10cm to receive distance, and relative humidity is 55%.
Prepared blended fiber felt fracture strength is 1.9MPa, and initial moduluss are 260MPa.The blended fiber felt is fixed
Length is soaked in 90vol% ethanol waters after 30min, and its fracture strength is 7.5MPa, and initial moduluss are 420MPa, energy to failure
For 120J Kg-1；The porosity of the blended fiber felt is 73%, and the scope for constituting the blended fiber diameter of fiber felt is
400nm～1.8 μm.
Embodiment 6
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.0mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 30% regenerated silk fibroin water solution；
(2) hydroxyethyl chitosan is dissolved in deionized water, prepares the hydroxyethyl chitosan water that mass percent is 4%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/hydroxyl with the hydroxyethyl chitosan aqueous solution
Ethyl chitosan mass ratio is 100:3 mixing, prepare regenerated silk fibroin/hydroxyethyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/hydroxyethyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain regenerated silk fibroin/hydroxyethyl chitosan blended fiber felt.Electrospinning conditions are：Spinning voltage is
25kV, spinning flow velocity are 1mL/h, and it is 10cm to receive distance, and relative humidity is 50%.
Prepared blended fiber felt fracture strength is 2.8MPa, and initial moduluss are 150MPa.The blended fiber felt is fixed
Length is soaked in 90vol% ethanol waters after 30min, and its fracture strength is 7.3MPa, and initial moduluss are 370MPa, energy to failure
For 60J Kg-1；The porosity of the blended fiber felt is 76%, and the scope for constituting the blended fiber diameter of fiber felt is 400nm
～1.6 μm.
Embodiment 7
A kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, its step is：
(1), with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, it is dissolved in mole
During concentration is for the lithium bromide water solution of 9.0mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 30% regenerated silk fibroin water solution；
(2) carboxymethyl chitosan is dissolved in deionized water, prepares the carboxymethyl chitosan sucrose solution that mass percent is 5%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/carboxylic with the carboxymethyl chitosan sugar aqueous solution
Methyl chitosan mass ratio is 100:5 mixing, prepare regenerated silk fibroin/carboxymethyl chitosan blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/carboxymethyl chitosan prepared by step (3), is carried out
Electrostatic spinning, that is, obtain regenerated silk fibroin/carboxymethyl chitosan blended fiber felt.Electrospinning conditions are：Spinning voltage is
30kV, spinning flow velocity are 1mL/h, and it is 15cm to receive distance, and relative humidity is 50%.
Prepared blended fiber felt fracture strength is 1.8MPa, and initial moduluss are 210MPa.The blended fiber felt is fixed
Length is soaked in 95vol% ethanol waters after 30min, and its fracture strength is 4.7MPa, and initial moduluss are 420MPa, energy to failure
For 98J Kg-1；The porosity of the blended fiber felt is 65%, and the scope for constituting the blended fiber diameter of fiber felt is 200nm
～1.4 μm.
Claims (6)
1. a kind of preparation method of regenerated silk fibroin/chitosan derivatives blended fiber felt, is characterized in that comprising the steps：
(1) with Bombyx bombycis as raw material, after Bombyx bombycis are with the aqueous sodium carbonate degumming of mass percent 0.5%, molar concentration is dissolved in
In for the lithium bromide water solution of 9.0～9.3mol/L；After by this solution centrifugal, filtration, dialysis and concentration, mass percent is obtained
For 25～35% regenerated silk fibroin water solutions；
(2) chitosan derivatives are dissolved in deionized water, prepare the chitosan derivatives water that mass percent is 1～5%
Solution；
(3) regenerated silk fibroin water solution is pressed regenerated silk fibroin/shitosan with the chitosan derivatives aqueous solution
Derivant mass ratio is 100:1～5 mixing, prepares regenerated silk fibroin/chitosan derivatives blending aqueous solution；
(4) aqueous solution is blended as spinning solution with regenerated silk fibroin/chitosan derivatives prepared by step (3), carries out electrostatic
Spinning, that is, obtain regenerated silk fibroin/chitosan derivatives blended fiber felt；
In the blended fiber felt, the weight/mass percentage composition of chitosan derivatives is 1～5%, and remaining is regenerated silk fibroin；Institute
Blended fiber felt fracture strength is stated for 1.75～2.8MPa, initial moduluss are 70～280MPa.
2. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its
It is characterised by, in step (4), electrospinning conditions are：Spinning voltage be 15～30kV, spinning flow velocity be 0.1～
1.0mL/h, it is 5～15cm to receive distance, and relative humidity is 45～55%.
3. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its
Be characterised by, after the blended fiber felt is through Ethanol Treatment, its fracture strength be 4～7.5MPa, initial moduluss be 360～
450MPa, energy to failure are 60～120J Kg-1.
4. the preparation method of a kind of regenerated silk fibroin according to claim 3/chitosan derivatives blended fiber felt, its
It is characterised by, described referring to through Ethanol Treatment is soaked in blended fiber felt fixed length 10 in 80～95vol% ethanol waters
～30min.
5. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its
It is characterised by, described chitosan derivatives are hydroxypropyl chitosan, hydroxyethyl chitosan, carboxymethyl chitosan, carboxyethyl shell
One kind in polysaccharide.
6. the preparation method of a kind of regenerated silk fibroin according to claim 1/chitosan derivatives blended fiber felt, its
It is characterised by, the porosity of the blended fiber felt is 65～80%, and the scope for constituting the blended fiber diameter of fiber felt is
200nm～2.4 μm.
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| WO2016023140A1 (en) * | 2014-08-15 | 2016-02-18 | 苏州堪赛尔生物技术有限公司 | Matrix scaffold for three-dimensional cell culturing and construction method and use thereof |
| CN106693030A (en) * | 2015-08-31 | 2017-05-24 | 青岛新智源健康科技有限公司 | Chitosan nanofibers as well as large-scale electrospinning preparation method and application thereof |
| CN107118361B (en) * | 2017-04-21 | 2020-04-28 | 浙江大学 | Silk fibroin/carboxymethyl chitosan composite gel and preparation method thereof |
| CN107012525A (en) * | 2017-06-01 | 2017-08-04 | 合肥创沃科技有限公司 | A kind of preparation method of the composite regenerated cellulose tissue repair materials of chitosan |
| CN109989119A (en) * | 2018-01-03 | 2019-07-09 | 浙江大学 | A kind of preparation method and product and application with the porous fibre for being orientated pore structure |
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| CN111188194B (en) * | 2020-02-24 | 2022-03-22 | 淮阴工学院 | Preparation method of silk fibroin/gelatin fiber reinforced chitosan composite hemostatic material |
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| CN103861145A (en) * | 2014-03-10 | 2014-06-18 | 武汉大学 | Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket |
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| CN102936794A (en) * | 2012-11-20 | 2013-02-20 | 东华大学 | Method for preparing composite nanofiber membrane based on natural material silk fibroin and chitosan |
| CN103861145A (en) * | 2014-03-10 | 2014-06-18 | 武汉大学 | Immediately crosslinking technology for preparing macroporous three-dimensional nanofiber bracket |
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