CN103989865A - Compound powder for treating fish sporozoan disease and preparation method thereof - Google Patents
Compound powder for treating fish sporozoan disease and preparation method thereof Download PDFInfo
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- CN103989865A CN103989865A CN201410202523.4A CN201410202523A CN103989865A CN 103989865 A CN103989865 A CN 103989865A CN 201410202523 A CN201410202523 A CN 201410202523A CN 103989865 A CN103989865 A CN 103989865A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to compound powder for treating fish sporozoan disease and a preparation method thereof. The compound powder comprises the following medicinal components in parts by weight: 5 to 10 parts of turmerone, 10 to 20 parts of astragalus polysaccharide, 3 to 5 parts of laccocephalum mylittae, 1 to 5 parts of halofuginone, 1 to 5 parts of plant flavonoid, 2 to 5 parts of betel nut, 15 to 20 parts of radix sophorae flavescentis, and 40 to 50 parts of glucose. The preparation method comprises the following steps of: crushing turmerone, astragalus polysaccharide, laccocephalum mylittae, halofuginone, plant flavonoid, betel nut, radix sophorae flavescentis, and glucose; screening by using a 60-mesh screen; weighing the components in parts by weight above; and uniformly mixing at normal temperature. The compound powder is clear in components, stable in effect, convenient to use, high in bioavailability, can be directly orally taken, is not influenced by weather and environment conditions, and has the effects of quickly recovering fish body health and appetite, decreasing sporozoan, controlling death of fish caused by sporozoan, and recovering fish to normal quickly after being taken.
Description
Technical field
The present invention relates to a kind of compounding powder for the treatment of Fish sporozoon disease and preparation method thereof, belong to Aquatic product disease control cultural technique field.
Background technology
Myxosporidiasis is very serious to China's aquaculture harm, the present invention intends with the Myxosporidiasis of Crucian Carp object of study of attaching most importance to, by the research to the screening of myxosporidiasis epidemiology, ecology, molecular biology and control medicine, develop the effective ecological prevention and control measure of myxosporidiasis and medicine.Carassius auratus is one of main large freshwater fish of China, because its delicious meat is loved by the people.Jiangsu Province is the main cultivation area of China Carassius auratus (Carassius aurutus gibelio), and high density culture is grown area and reached more than 1,600,000 mu, approximately 800,000 tons of annual productions, approximately more than 60 hundred million yuan of the output values.At present, crucian cultivation has become the important industry of North Jiangsu Area agricultural byproducts of Jiangsu Province-particularly.Crucian cultivation industry expanding fisherman's employment, solve surplus rural labor force, the aspect such as increase farmers' income, make the rural economy flourish plays a significant role.Yet along with continuous expansion and the high density culture of industry size, the problems such as seed degeneration, disease, cultivation water environment deterioration, Product quality and safety are having a strong impact on the sustainable health development of crucian cultivation industry day by day.This wherein endangers Carassius auratus Myxosporidia From Freshwater disease most serious of all.From the nineties, the crucian cultivation industry of this area suffers the serious puzzlement of myxosporidiasis always, has the report of Carassius auratus mortality every year, and local fishery cultivating has been caused to huge economic loss.
The control strategy of parasite disease and medicament research and development depend on the research of the biological characteristicses such as its complete life cycle, as the research of the controls such as malaria, schistosomicide.Fish parasitic disease is no exception.Yet China still belongs to blank to the research of Myxosporean life cycle, and only from water body in lake, Branchiura sowerbyi has been reported a kind of Triactinosporean so far, and its corresponding gloeospore does not detect (Wang Guitang etc., 2000).The research of the benthic fauna, bottom fauna of freshwater aquiculture water body is also very lacked.Up to date, just by our system investigation Myxosporean morbidity pond and the end in the non-morbidity pond oligochaetes of dwelling form, the end of the finding morbidity pond oligochaetes of dwelling is single, but not the pond multiformity of falling ill is higher.And the dwell parasitic Actinosporean of oligochaetes of the end in morbidity pond is investigated, find altogether three kinds of dissimilar Actinosporeans, Branchiura sowerbyi is its main host plants.But the Myxosporean kind of China's freshwater aquiculture kind and infection is various, need large-scale further investigation, to grasp in detail the most serious Myxosporean biological characteristics of harm in cultivation.
It is main study subject that this project be take the more serious Carassius aurutus gibelio myxosporidiasis of Yancheng, Jiangsu Province area harm, grasping its cause of disease macrocyst iodine spore worm (Myxobolus ampullicapulus comprehensively, this cause of disease is being determined by form and molecular biology method in the recent period by fresh water fishery research center) life cycle, the growth in host and the process of dividing a word with a hyphen at the end of a line, disease occur with the basis such as breeding water body environment physical and chemical factor above, explore the Myxosporean disease of ecology and two aspects of medicine control and prevent and treat method.Bionomic control research, intend by the diagnosis of molecular biology method of development full life cycle phase of sensitive this Myxosporean efficiently, investigate this Myxosporean to appeal difference, difference (or the different pedigree) end of the different growth stages of the host fish susceptibility difference of oligochaetes to this Myxosporean of dwelling, the research on the impact of susceptibility oligochaetes etc. of water body physical and chemical factor and substrate, reaches and reduces the object that disease occurs thereby block the growth of this Myxosporean in host and propagation by Ecological regulation method.Medical treatment, kills the drug candidate of this Myxosporean different phase by screening, inquire into drug candidate to allogynogenetic crucian carp, ingest, growth, the physiological parameter such as behavior.In laboratory and clinical two aspects, by observation in vitro, pathologic finding and the removing of this Myxosporean of molecular diagnosis method research drug candidate, effect and the fish body recovery situation that blocking-up is grown, determine that the suitableeest drug level, administering mode (splashing, mix bait), administration cycle, off-drug period etc. are with the nuisanceless medicine control strategy of development Carassius aurutus gibelio " larynx sporidiosis ".
By carrying out of this project, to promoting China's Myxosporean biological study to have positive effect, more solve puzzlement China cultured freshwater fish Myxosporean disease problem, promotion industry develops in a healthy way provides important science and technology support.Effective control to Myxosporean disease, will retrieve huge economic loss for our province; Moreover, science, effectively control method, by greatly reducing the cost of disease control, greatly alleviating medicine to the pollution of water body and harm that the mankind are caused, therefore has huge economic worth and social influence.After the promotion and implementation of expectation project, will be every year for our province freshwater aquaculture industry is retrieved economic losses or increase by 100,000,000 yuans of left and right of economic benefit.
Radiation spore is invaded fish body by the gill, epidermis, the intestinal of fish body, discharges sporonin; Then in interstice or blood, nervous system, travel to suitable parasitic site.Its intrusion of different Myxosporean kinds, the mode of dividing a word with a hyphen at the end of a line are not quite similar.As " UBO " that find in Cyprinus carpio blood is in early days widely regarded as the early development form of Myxosporean in blood.Recently, the application of immuning hybridization technology has confirmed that many Myxosporeans exist the blood phase, and by extraction, infecting the new fish body of fish blood infection also provides strong evidence for Myxosporean exists the blood phase.Yet blood phase life period needs further to be studied.Myxosporean divides 2 stages in the growth course in the parasitic site of fish body, activity takes food vegetative stage and inactive Spore Stages. the former lives in host's tissue lumen or cell tissue, form is various, main function be in pin main body, absorb nourishment to grow, growth, asexual reproduction.The latter forms fixedly spore of form in the host endoparasitism later stage, and main function is the destruction that in protection spore, idioplasm is not subject to external environment, guarantees that idioplasm infects next host.When actual generation Fish myxosporidiasis, what be conventionally checked through is all the Spore Stages of Myxosporean maturation, and now a large amount of spores are dispersed in host tissue gap or are coated with and form white sporangiocyst by host's connective tissue.Ripe spore is comprised of valve, utmost point capsule and the idioplasm of protoplasm specialization.2 or the hard valve of multi-disc are closely sewed up, and intracavity is comprising utmost point capsule and idioplasm.The mechanism of firmly sewing up for valve at present it be unclear that.It is bonding by a kind of special " protoplasm condenses " between shell sheet that Keysselitz (1908) thinks.Xu Haisheng and Wang Shuxia (2001) find there is a large amount of microtubules between silver carp myxobolus shell sheet gap by electron microscopic observation.In addition, Lom (1964) research points out that the shell sheet of gloeospore punching can not be dissolved by trypsin and pepsin.Kudo(1921) discovery shell sheet boils in dilute sulfuric acid and 35% potassium hydroxide solution does not all have to dissolve.Therefore, general medicine is difficult to destroy and kill ripe spore.In the control Myxosporean method proposing at present, all medicine is killed to trophosome as Main Preventive Measures, simultaneously in conjunction with bionomic control.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of compounding powder for the treatment of Fish sporozoon disease and preparation method thereof is provided, described compounding powder definite ingredients, effect stability, can directly take orally, easy to use, bioavailability is high, is not subject to the impact of weather environment condition; After use, can recover rapidly fish body body constitution and appetite, reduce sporozoon quantity, control the fish kills causing because of sporozoon, make fish recover as early as possible normal.
According to technical scheme provided by the invention: a kind of compounding powder for the treatment of Fish sporozoon disease, is characterized in that: the drug component that described compound preparation comprises and the parts by weight of each component are: turmerone 5-10 part, astragalus polysaccharides 10-20 part, Omphalia 3-5 part, halofuginone hydrobromide 1-5 part, phytol ketone 1-5 part, Semen Arecae 2-5 part, Radix Sophorae Flavescentis 15-20 part, glucose 40-50 part.
A kind of preparation method for the treatment of the compounding powder of Fish sporozoon disease, it is characterized in that: first turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then by above-mentioned weight proportion, take each component, mix homogeneously, makes the compounding powder for the treatment of Fish sporozoon disease at normal temperatures.
the present invention compared with prior art, has the following advantages:
(1) compound preparation definite ingredients of the present invention, effect stability, can directly take orally, and easy to use, bioavailability is high, is not subject to the impact of weather environment condition; After use, can recover rapidly fish body body constitution and appetite, reduce sporozoon quantity, within 3-5 days, can control the fish kills causing because of sporozoon, make fish recover as early as possible normal.
(2) compound preparation of the present invention utilizes the parasitic sugar that becomes, and with sugar, as food calling, also impels fish, astragalus polysaccharides can strengthen again fish body body constitution and makes Fish normally ingest to allow medicine effectively kill parasite simultaneously.
(3) each drug component in compound preparation of the present invention is to aquatic animal safety non-toxic environmental protection noresidues such as fish shrimp crabs, do not affect fish and normally ingests killing the parasitic fish body body constitution that strengthens simultaneously, and impel healthy growth.
The specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1:
By following weight proportion, take each drug component:
5 parts of turmerones, 20 parts of astragalus polysaccharidess, 3 parts of Omphalias, 5 parts of halofuginone hydrobromides, 5 parts of phytol ketones, Semen Arecae 2,15 parts of Radix Sophorae Flavescentiss, 50 parts of glucoses.
First turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then each component is put into mixer, be uniformly mixed at normal temperatures, make the compounding powder for the treatment of Fish sporozoon disease.
The compounding powder that embodiment 1 is made is applied in two ponds of the other bridge aquatic farm in Liyang, cultivation adult fish of crucian in a pond, and cultured area is 200 mu, 2 meters of mean depths; Cultivation fingerling in another pond, cultured area is 30 mu, 1.5 meters of mean depths; All there is sporidiosis in two ponds, naked eyes are visible, and sampling microscopy is found a large amount of live body iodine spore worms, adult fish of crucian dead 2000 tail left and right every day, fingerling dead 3000 tail left and right every day.
The compounding powder that embodiment 1 is made is mixed and is sneaked in daily breeding feed by 0.5% part by weight, and breeding feed per ton adds 5kg compounding powder medicine.Throw something and feed every day 2 times, throw something and feed the 3rd day time, fish mortality starts to decline, and within the 4th day, mortality obviously reduces by half, and to the 7th day macrura reevesii, stops death, and fish food ration recovers normal, and the microscopy of sampling after first quarter moon, does not find sporozoon again.
Embodiment 2:
By following weight proportion, take each drug component:
10 parts of turmerones, 10 parts of astragalus polysaccharidess, 5 parts of Omphalias, 1 part of halofuginone hydrobromide, 5 parts of phytol ketones, 5 parts of Semen Arecaes, 20 parts of Radix Sophorae Flavescentiss, 40 parts of glucoses.
First turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then each component is put into mixer, be uniformly mixed at normal temperatures, make the compounding powder for the treatment of Fish sporozoon disease.
The compounding powder that embodiment 2 is made is applied in the fingerling pond of Hengshan Mountain, Wujin, Changzhou Qiao Yi fingerling plant, 15 mu of this fingerling pond cultured areas, mean depth 1.5m, there is sporidiosis, naked eyes are visible, and microscopy is found a large amount of live body iodine spore worms, fingerling dead 1200 tail left and right every day.
The compounding powder of embodiment 2 preparations is mixed and sneaked in daily breeding feed by 0.5% part by weight, throw something and feed every day 2 times, throw something and feed the 4th day time, fish mortality starts to decline, within the 5th day, mortality obviously reduces by half, and to the 9th day macrura reevesii, stops death, and fish food ration recovers normal, the microscopy of sampling after first quarter moon, does not find sporozoon again.
Embodiment 3:
By following weight proportion, take each drug component:
7 parts of turmerones, 15 parts of astragalus polysaccharidess, 4 parts of Omphalias, 3 parts of halofuginone hydrobromides, 3 parts of phytol ketones, 3 parts of Semen Arecaes, 18 parts of Radix Sophorae Flavescentiss, 45 parts of glucoses.
First turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then each component is put into mixer, be uniformly mixed at normal temperatures, make the compounding powder for the treatment of Fish sporozoon disease.
The compounding powder that embodiment 3 is made is applied to one Cyprinus carpio L. fish culture field, Wuxi, and the Cyprinus carpio L. fingerling of this plant is found sporozoon, and fish is kept afloat, and has fragmentary death.
The compounding powder that embodiment 3 is made is mixed and is sneaked in daily breeding feed by 0.5% part by weight, throws something and feeds every day 2 times, throws something and feeds and just no longer includes afterwards fish death in 2 days, does not also have fish to float on the surface, and after 4 days, fish recovers normal.
Embodiment 4:
By following weight proportion, take each drug component:
10 parts of turmerones, 20 parts of astragalus polysaccharidess, 5 parts of Omphalias, 5 parts of halofuginone hydrobromides, 5 parts of phytol ketones, 5 parts of Semen Arecaes, 20 parts of Radix Sophorae Flavescentiss, 50 parts of glucoses.
First turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then each component is put into mixer, be uniformly mixed at normal temperatures, make the compounding powder for the treatment of Fish sporozoon disease.
The compounding powder that embodiment 4 is made is applied in the crucian cultivation pond of Jiangsu great Feng mono-plant, 400 mu of this crucian cultivation pond areas, mean depth 1.5m, there is sporidiosis, naked eyes are visible, and microscopy is found a large amount of live body iodine spore worms, fish dead 2000 jin of left and right every day.
The compounding powder of embodiment 4 preparation is mixed and sneaked in daily breeding feed by 0.5% part by weight, throw something and feed every day 2 times, throw something and feed the 3rd day time, fish mortality starts to decline, within the 5th day, mortality obviously reduces by half, and to the 8th day macrura reevesii, stops death, and fish food ration recovers normal.
Claims (2)
1. a compounding powder for the treatment of Fish sporozoon disease, is characterized in that: the drug component that described compound preparation comprises and the parts by weight of each component are: turmerone 5-10 part, astragalus polysaccharides 10-20 part, Omphalia 3-5 part, halofuginone hydrobromide 1-5 part, phytol ketone 1-5 part, Semen Arecae 2-5 part, Radix Sophorae Flavescentis 15-20 part, glucose 40-50 part.
2. the preparation method of the compounding powder of a treatment claimed in claim 1 Fish sporozoon disease, it is characterized in that: first turmerone, astragalus polysaccharides, Omphalia, halofuginone hydrobromide, phytol ketone, Semen Arecae, Radix Sophorae Flavescentis, glucose are pulverized, with 60 mesh sieves, screen, then by above-mentioned weight proportion, take each component, mix homogeneously, makes the compounding powder for the treatment of Fish sporozoon disease at normal temperatures.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106692369A (en) * | 2017-03-06 | 2017-05-24 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating sporidiosis of fishes |
| CN108721345A (en) * | 2018-08-22 | 2018-11-02 | 福建省淡水水产研究所 | A kind of compound formulation for treating large fresh-water fishes ich |
| CN115444853A (en) * | 2022-10-25 | 2022-12-09 | 宁波大学 | Method for preventing and treating pathogenic ciliates of aquatic animals |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690767A (en) * | 2009-09-29 | 2010-04-07 | 北京大北农动物保健科技有限责任公司 | Compound pesticide for fishing and preparation method and application thereof |
| CN103623071A (en) * | 2013-12-03 | 2014-03-12 | 湖北武当动物药业有限责任公司 | Powerful insect repellent for veterinary use and preparation method thereof |
-
2014
- 2014-05-14 CN CN201410202523.4A patent/CN103989865A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690767A (en) * | 2009-09-29 | 2010-04-07 | 北京大北农动物保健科技有限责任公司 | Compound pesticide for fishing and preparation method and application thereof |
| CN103623071A (en) * | 2013-12-03 | 2014-03-12 | 湖北武当动物药业有限责任公司 | Powerful insect repellent for veterinary use and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 秦建华，等: "《动物寄生虫病学》", 31 July 2013 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106692369A (en) * | 2017-03-06 | 2017-05-24 | 长沙瑞多康生物科技有限公司 | Composition for preventing and treating sporidiosis of fishes |
| CN108721345A (en) * | 2018-08-22 | 2018-11-02 | 福建省淡水水产研究所 | A kind of compound formulation for treating large fresh-water fishes ich |
| CN115444853A (en) * | 2022-10-25 | 2022-12-09 | 宁波大学 | Method for preventing and treating pathogenic ciliates of aquatic animals |
| CN115444853B (en) * | 2022-10-25 | 2023-08-04 | 宁波大学 | Method for preventing and controlling pathogenic ciliates of aquatic animals |
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