CN103988847B - Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane - Google Patents
Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane Download PDFInfo
- Publication number
- CN103988847B CN103988847B CN201410249256.6A CN201410249256A CN103988847B CN 103988847 B CN103988847 B CN 103988847B CN 201410249256 A CN201410249256 A CN 201410249256A CN 103988847 B CN103988847 B CN 103988847B
- Authority
- CN
- China
- Prior art keywords
- dithiocyano
- composition
- methane
- chitosan oligosaccharide
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 65
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 title claims abstract description 35
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 8
- 201000010099 disease Diseases 0.000 claims abstract description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 37
- 240000007594 Oryza sativa Species 0.000 claims abstract description 18
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 10
- 239000006002 Pepper Substances 0.000 claims abstract description 7
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 7
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 7
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 7
- 241000244206 Nematoda Species 0.000 claims abstract description 5
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 3
- 241000223218 Fusarium Species 0.000 claims abstract description 3
- 241000243785 Meloidogyne javanica Species 0.000 claims description 12
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 claims description 11
- 240000005561 Musa balbisiana Species 0.000 claims description 9
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 9
- 240000008067 Cucumis sativus Species 0.000 claims description 8
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 8
- 240000003889 Piper guineense Species 0.000 claims description 8
- 241001530056 Athelia rolfsii Species 0.000 claims description 5
- 241000700605 Viruses Species 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 4
- 240000008574 Capsicum frutescens Species 0.000 claims description 3
- 239000001390 capsicum minimum Substances 0.000 claims description 3
- 240000007087 Apium graveolens Species 0.000 claims description 2
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 claims description 2
- 235000010591 Appio Nutrition 0.000 claims description 2
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 2
- 244000105624 Arachis hypogaea Species 0.000 claims description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 2
- 235000018262 Arachis monticola Nutrition 0.000 claims description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 2
- 241000335053 Beta vulgaris Species 0.000 claims description 2
- 241000123650 Botrytis cinerea Species 0.000 claims description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 2
- 241000233652 Chytridiomycota Species 0.000 claims description 2
- 241000675108 Citrus tangerina Species 0.000 claims description 2
- 244000060011 Cocos nucifera Species 0.000 claims description 2
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 2
- 244000301850 Cupressus sempervirens Species 0.000 claims description 2
- 241000221785 Erysiphales Species 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 241000220225 Malus Species 0.000 claims description 2
- 235000011430 Malus pumila Nutrition 0.000 claims description 2
- 235000015103 Malus silvestris Nutrition 0.000 claims description 2
- 244000061176 Nicotiana tabacum Species 0.000 claims description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 2
- 241000233679 Peronosporaceae Species 0.000 claims description 2
- 244000082204 Phyllostachys viridis Species 0.000 claims description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 2
- 241000018646 Pinus brutia Species 0.000 claims description 2
- 235000011613 Pinus brutia Nutrition 0.000 claims description 2
- 240000001987 Pyrus communis Species 0.000 claims description 2
- 235000014443 Pyrus communis Nutrition 0.000 claims description 2
- 241000124033 Salix Species 0.000 claims description 2
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 claims description 2
- 235000009337 Spinacia oleracea Nutrition 0.000 claims description 2
- 244000300264 Spinacia oleracea Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 239000011425 bamboo Substances 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 2
- 235000020232 peanut Nutrition 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 244000241235 Citrullus lanatus Species 0.000 claims 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 8
- 241000589771 Ralstonia solanacearum Species 0.000 abstract description 2
- 244000203593 Piper nigrum Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 32
- 230000003449 preventive effect Effects 0.000 description 16
- 241000219109 Citrullus Species 0.000 description 10
- 230000002195 synergetic effect Effects 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- OMOVVBIIQSXZSZ-UHFFFAOYSA-N [6-(4-acetyloxy-5,9a-dimethyl-2,7-dioxo-4,5a,6,9-tetrahydro-3h-pyrano[3,4-b]oxepin-5-yl)-5-formyloxy-3-(furan-3-yl)-3a-methyl-7-methylidene-1a,2,3,4,5,6-hexahydroindeno[1,7a-b]oxiren-4-yl] 2-hydroxy-3-methylpentanoate Chemical compound CC12C(OC(=O)C(O)C(C)CC)C(OC=O)C(C3(C)C(CC(=O)OC4(C)COC(=O)CC43)OC(C)=O)C(=C)C32OC3CC1C=1C=COC=1 OMOVVBIIQSXZSZ-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000243786 Meloidogyne incognita Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 240000000359 Triticum dicoccon Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to plant protection art, be specifically related to the bactericidal composition of chitosan oligosaccharide and dithiocyano-methane.In composition, the mass ratio of dithiocyano-methane and chitosan oligosaccharide is 40:1-1:40, the percentage that the quality summation of chitosan oligosaccharide and dithiocyano-methane accounts for composition gross mass is 0.1-80%, and composition is for preventing and treating nematode, fusarium wilt, bakanae disease of rice and pepper ralstonia solanacearum etc.
Description
Technical field
The invention belongs to plant protection art, particularly relate to the bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane and purposes.
Background technology
Along with the change of cropping system, the occurrence and infection of plant disease is on the rise, and the pesticide resistance of cause of disease to bactericide also improves thereupon, and exploring agricultural chemicals that is efficient, low toxicity becomes one of hot issue of related scientific research and managing and serving system concern.Chitosan oligosaccharide, in the process resisting pathogen erosion, can be induced and excite plant to produce a series of defense reaction, and these defense reactions synergy stops propagation and the invasion of pathogen, reaches the effect of preventing and curing diseases.Dithiocyano-methane (MBT) is that one efficiently kills algae sterilization chemicals, MBT has efficient killing effect for the main bacteria existed in cycle water, fungi and algae (all kinds of fungi), and duration of efficacy is long, the pH value of adaptation and wider range.Use the consumption of 3-4PPM to the sterilizing rate of heterotroph, nitrite bacteria, sulfosalt reducing bacteria, Escherichia coli, iron wire bacterium, anaerobic bacteria, denitrifying bacterium and sulphur bacteria up to more than 99%, also can reach high sterilizing rate to other all kinds of bacterium.In all kinds of fungi of removing, dithiocyano-methane is than the better effects if of its homicide/degerming medicine.Its stoste is stablized, and can degrade rapidly or decompose again, can not cause secondary pollution, highly-effective low-residue to environment when applying.To bakanae disease of rice and Aplelenchiodes bessegi of rice, stripe disease of barley, net blotch, covered smut etc., there is higher preventive effect.Also there is good preventive effect to kind of other disease passed caused by nematode, bacterium and fungi simultaneously, under recommended dose, to rice Wheat Seeds safety, and have certain effect to raising Germination Percentage of Rice and percentage establishment of seedling.
Inventor has carried out large quantity research to the composition containing chitosan oligosaccharide and dithiocyano-methane, has found some unexpected effects, so complete the present invention.
Summary of the invention
The object of this invention is to provide bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane with synergistic effect and uses thereof.
Concrete, the invention provides the bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane, wherein the mass ratio of chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40, and preferably, mass ratio is 10:1-1:10.
In above-mentioned composition, the percentage that the quality summation of chitosan oligosaccharide and dithiocyano-methane accounts for composition gross mass is 0.1-80%.
Above-mentioned composition, is characterized in that composition is with seed dressing, spraying, filling root, immersion applications.
Present invention also offers above-mentioned composition for preventing and treating the purposes of crop pest, wherein said crop is cereal crops, vegetables, forest or other crops, and cereal crops are paddy rice, peanut, corn, soybean, potato, Chinese sorghum or wheat; Vegetables are cucumber, capsicum, tomato, celery, spinach, Chinese cabbage or beet; Forest is willow, pine tree, cypress, cocoanut tree or bamboo grows, and other crops are banana, watermelon, apple, pear tree, tobacco or oranges and tangerines.
In such use, the disease of suitable control comprises: nemas, virus disease, the fungus-caused fusarium wilt of Deuteromycotina, gray mold etc., the fungus-caused bakanae disease of Ascomycotina, powdery mildew, stalk break etc., the banded sclerotial blight that Basidiomycotina causes, rust, smut etc., the fungus-caused downy mildew of Mastigomycotina, late blight etc.
The purposes of foregoing, preferably, disease is banana root-knot nematode, cucumber root-knot nematode, watermelon blight, bakanae disease of rice and banded sclerotial blight, pepper virus disease, Botrytis cinerea and pepper ralstonia solanacearum etc.
Beneficial effect of the present invention is:
1, synergistic effect is obvious: composition shows good synergistic effect in certain ratio range, improves the protection effect of composition.
2, expanding prevention spectrum: composition can prevent and treat the multiple diseases in agricultural production.
3, use cost is reduced: because of composite, there is raising preventive effect, the increase of control spectrum, decrease access times, reduce use cost;
4, delay pesticide resistance: the composition of two kinds of different mechanism of action is composite, and pathogen not easily develops immunity to drugs, delay pathogen to the drug-fast generation of single dose.
Embodiment
The following stated, only for explaining the present invention, for not departing from amendment that the present invention's spirit and principle make, replacement or improvement, all belongs to the scope of protection of present invention.
The composition of embodiment 1 chitosan oligosaccharide and dithiocyano-methane is to the effect of banana root-knot nematode
The composition of dithiocyano-methane single dose, chitosan oligosaccharide single dose and certain mass ratio is diluted to certain variable concentrations.Drip on slide by the quantitative dilution containing banana root-knot nematode, microscopy counts; Microscopy is mixed into the root-knot nematode dilution of quantitative pharmacy in the same way, and microscopy counts, and measures each reagent agent lethal concentration of 50 LC respectively
50, according to the co-toxicity coefficient (CTC value) of the abundant method calculation composition of Sun Yun, CTC value be greater than 120 for synergistic effect, concrete grammar is as follows.
Set a kind of single dose (A) for standard single dose, specify its toxicity index (TI) to be 100, then:
The percentage of TI × single dose in mixture of theoretical toxicity index (the TTI)=each single dose of Σ of mixture
Result is as following table 1.
The composition of table 1 chitosan oligosaccharide and dithiocyano-methane is to the synergistic effect of banana root-knot nematode
As can be seen from Table 1, when the proportioning of chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40, the co-toxicity coefficient of composition to banana root-knot nematode is all greater than 120, illustrates that the synergistic effect of the composition of chitosan oligosaccharide and dithiocyano-methane to banana root-knot nematode is obvious.
The composition of embodiment 2 chitosan oligosaccharide and dithiocyano-methane is to the preventive effect of cucumber nematode
Compost is mixed into sterilized soil and sterilizing river sand, be mixed into composition or single dose, fully mix thoroughly, add clear water and make water content 30%, load basin alms bowl, have the cucumber seedling of 2 true leaves to be transplanted in basin growth, transplant latter 7 days, every basin access Meloidogyne incognita 2 instar larvae 200, be placed in greenhouse, quantitative watering manages.If often process 3 repetitions, and set and be not mixed into chemicals treatment as blank.After 20 days, when blank plant rhizosphere has a large amount of root knot, investigation plant height and preventive effect.Record every strain root knot number, and carry out classification by Liu Weizhi (1995): 0 grade, without root knot; 1 grade, 1-2 root knot; 2 grades, 3-10 root knot; 3 grades, 11-30 root knot; 4 grades, 31-100 root knot; 5 grades, more than 100 root knots.The results are shown in Table 2.
The composition of table 2 chitosan oligosaccharide and dithiocyano-methane is to the effect of cucumber root-knot nematode
Table 2 shows, relative to corresponding single dose and blank, the process plant height of the composition of chitosan oligosaccharide and dithiocyano-methane significantly improves, and root knot number obviously reduces, and disease obviously alleviates.Illustrate that composition is obvious to the control efficiency of cucumber root-knot nematode, promote plant growth.
The composition of embodiment 3 chitosan oligosaccharide and dithiocyano-methane is to the effect of watermelon blight
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to trial test, by the concentration that Formulation becomes to need.Before selecting, stubble watermelon blight morbidity field, arranges field evenly.Evenly dress seed (concentration is with effective milligram of gauge of every kilogram of seed with medicament) with the suspension seed-coating agent of different agents, sow, Routine Management.Watermelon plant diseases situation is investigated when disease onset.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, the effect of the substance A that x is concentration when being a, the effect of the substance B that y is concentration when being b, compares theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.Composition the results are shown in Table 3 to watermelon disease.
The composition of table 3 chitosan oligosaccharide and dithiocyano-methane is to the effect of watermelon blight
By the test of composition to watermelon blight, as seen from Table 3, the composition of chitosan oligosaccharide and dithiocyano-methane is to the theoretical preventive effect of the effect of watermelon blight higher than correspondent composition.Obviously, the composition of chitosan oligosaccharide and dithiocyano-methane is synergy to watermelon blight.
The composition of embodiment 4 chitosan oligosaccharide and dithiocyano-methane is to the effect of rice disease
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, by the concentration that Formulation becomes to need.Select once to fall ill rice field, carry out paddy rice seed dressing (concentration is with effective milligram of gauge of every kilogram of seed with medicament), sowing, seedling.Institute an inquiry when blank bakanae disease of rice, banded sclerotial blight evil occur, often process is by 5 samplings, often 50 strains, measures the percentage that plant blade face is infected.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, the effect of the substance A that x is concentration when being a, the effect of the substance B that y is concentration when being b, compares theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.Composition the results are shown in Table 4 to rice plant disease.
The composition of table 4 chitosan oligosaccharide and dithiocyano-methane is to the field efficacy of rice disease
As seen from Table 4, the composition of chitosan oligosaccharide and dithiocyano-methane when 1:1 and 4:1, composition to the actual effect of bakanae disease of rice, rice sheath blight disease all higher than the theoretical preventive effect of composition.Obviously, the composition of chitosan oligosaccharide and dithiocyano-methane is synergy to bakanae disease of rice, rice sheath blight disease.
The composition of embodiment 5 chitosan oligosaccharide and dithiocyano-methane is to the effect of pepper diseases
According to scheme, preparation built agent and unit dose preparation, and carry out active preliminary experiment.According to determination of activity trial test, by the concentration that Formulation becomes to need.Establish corresponding single dose and blank in capsicum field before test, adopt the conventional conventional spray of manual sprayer, every medicament sprays 50 strains.7-10d dispenser 1 time again after 1st dispenser.10-20d after 2nd dispenser, investigates pepper plant disease incidence of listing.Use Abbot formula, convert percentage to preventive effect.
In theoretical effect, the effect of the substance A that x is concentration when being a, the effect of the substance B that y is concentration when being b, compares theoretical effect and actual effect, evaluates the synergy of composition, for synergistic effect when being greater than theoretical preventive effect with actual preventive effect.Composition to the result of pepper plant disease respectively in table 5.
The composition of table 5 chitosan oligosaccharide and dithiocyano-methane is to the field efficacy of pepper diseases
As seen from Table 5, the composition containing chitosan oligosaccharide and dithiocyano-methane all higher than theoretical effect, shows that composition has obvious synergistic function to the actual effect of pepper virus disease, gray mold and bacterial wilt.
Claims (7)
1. contain the bactericidal composition of chitosan oligosaccharide and dithiocyano-methane, it is characterized in that the mass ratio of chitosan oligosaccharide and dithiocyano-methane is 40:1-1:40.
2. composition according to claim 1, is characterized in that chitosan oligosaccharide and dithiocyano-methane mass ratio are 10:1-1:10.
3. composition according to claim 2, is characterized in that the percentage that the quality summation of chitosan oligosaccharide and dithiocyano-methane accounts for composition gross mass is 0.1-80%.
4. composition according to claim 3, is characterized in that composition is with seed dressing, spraying, filling root, immersion applications.
5. composition according to claim 4 is for preventing and treating the purposes of crop pest, and wherein said crop is cereal crops, vegetables, forest or other crops, and cereal crops are paddy rice, peanut, corn, soybean, potato, Chinese sorghum or wheat; Vegetables are cucumber, capsicum, tomato, celery, spinach, Chinese cabbage or beet; Forest is willow, pine tree, cypress, cocoanut tree or bamboo grows, and other crops are banana, watermelon, apple, pear tree, tobacco or oranges and tangerines.
6. purposes according to claim 5, the disease of suitable control comprises: nemas, virus disease, the fungus-caused fusarium wilt of Deuteromycotina, gray mold, the fungus-caused bakanae disease of Ascomycotina, powdery mildew, stalk break, the banded sclerotial blight that Basidiomycotina causes, rust, smut, the fungus-caused downy mildew of Mastigomycotina, late blight.
7. purposes according to claim 6, disease is banana root-knot nematode, cucumber root-knot nematode, watermelon blight, bakanae disease of rice and banded sclerotial blight, pepper virus disease, Botrytis cinerea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410249256.6A CN103988847B (en) | 2014-06-07 | 2014-06-07 | Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410249256.6A CN103988847B (en) | 2014-06-07 | 2014-06-07 | Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103988847A CN103988847A (en) | 2014-08-20 |
| CN103988847B true CN103988847B (en) | 2015-12-09 |
Family
ID=51303391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410249256.6A Active CN103988847B (en) | 2014-06-07 | 2014-06-07 | Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103988847B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106305759B (en) * | 2016-08-22 | 2018-06-08 | 永春锐拓信息技术中心 | A kind of composition pesticide of fluorine-containing thiophene worm sulfone and dithiocyano-methane |
| CN110946149B (en) * | 2019-12-09 | 2021-11-30 | 王映镝 | Bactericidal and algae-killing synergistic composition for paint, coating, water body and wood and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210674A (en) * | 1998-07-28 | 1999-03-17 | 南京农业大学 | Method for preventing and controlling plant diseases in agriculture |
| CN101637177A (en) * | 2009-09-02 | 2010-02-03 | 云南省农业科学院农业环境资源研究所 | Preventing agent of erigeron breviscapus root rots and application thereof |
| CN102138554B (en) * | 2010-02-02 | 2015-03-11 | 梅州市凤兴环保科技有限公司 | Method for preparing methylene dithiocyanate emulsion serving as efficient bactericide |
-
2014
- 2014-06-07 CN CN201410249256.6A patent/CN103988847B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103988847A (en) | 2014-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101028009A (en) | Caprotrahol and Bacillus subtilis compound agricultural chemicals | |
| CN103283725A (en) | Sterilization composition containing amino-oligosaccharin and validamycin and application of pesticide composition | |
| CN104782633A (en) | Combination containing chitosan oligosaccharide and ipconazole or prothioconazole | |
| CN103271094A (en) | Compound bactericide | |
| CN102960359B (en) | Composition of dithiocyano-methane and macrolide insecticide component | |
| CN103300023A (en) | Pesticide composition | |
| KR101253712B1 (en) | Chitosan and chitooligosaccharide composition for preventing and disease control of plant disease and method for increase of plant growth and disease control of plant by using the same | |
| CN103988847B (en) | Bactericidal composition containing chitosan oligosaccharide and dithiocyano-methane | |
| CN103875712A (en) | Safe pleietrepie rice seed treating agent | |
| CN103828820B (en) | Bactericidal composition containing oligosaccharide and fluoxastrobin | |
| Wan Ahmad Sofian et al. | Foliar application of chitosan increases plant growth and ecofriendly control of Cucumis sativus leaf disease | |
| Pawar et al. | Management of seed/soil borne diseases of safflower by chemical and biocontrol agents | |
| CN105901001A (en) | Bactericide composition containing metconazole and hymexazol | |
| KR101385557B1 (en) | Chitosan and chitooligosaccharide composition for preventing and disease control of plant disease and method for increase of plant growth and disease control of plant by using the same | |
| CN102934639B (en) | Dithiocyano-methane/organophosphorus pesticide composition | |
| CN104106579B (en) | Compound bactericide for preventing and treating rice sheath blight | |
| CN104472507B (en) | A kind of Bactericide composition preventing and treating soil-borne disease | |
| CN109984152A (en) | Composition containing oligosaccharides and fragrance | |
| CN103070175B (en) | Compound pesticide containing boscalid and kasugamycin and application of compound pesticide | |
| CN107047584A (en) | Composition pesticide containing captan and Mancozeb | |
| Balicevic et al. | Control of soil parasites (Pythium debaryanum, Rizoctonia solani) on tomato by a biological product | |
| CN105165856A (en) | Bactericide containing carbendazim and cinnamaldehyde | |
| CN105145599A (en) | Bactericide containing thifluzamide and cinnamyl aldehyde | |
| CN105309461A (en) | Bactericide containing prochloraz and cinnamaldehyde | |
| CN104430383A (en) | Sterilization composition containing picoxystrobin and difenoconazole and application of composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate Effective date of registration: 20160822 Granted publication date: 20151209 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2016460000002 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20190605 Granted publication date: 20151209 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2016460000002 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Bactericidal composition containing chitosan oligosaccharide and methylene dithiocyanate Effective date of registration: 20190617 Granted publication date: 20151209 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Fuchang Village, Laocheng Development Zone, Chengmai County, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |