CN103980242A - Method for extracting moringa oleifera lam flavone from moringa oleifera lam leaves - Google Patents
Method for extracting moringa oleifera lam flavone from moringa oleifera lam leaves Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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Abstract
The invention relates to a method for extracting moringa oleifera lam flavone from moringa oleifera lam leaves. The method comprises the steps: 1) drying the moringa oleifera lam leaves, loading into a filter basket, putting into a sub-critical extraction kettle, and closing airtightly the extraction kettle; then adding an organic extraction agent, heating up until an extracted liquid in the sub-critical extraction kettle is in a sub-critical state; and extracting to obtain the extracted liquid, putting into a solvent recycling kettle, carrying out reduced-pressure recycling of the solvent, and thus obtaining a black green extract; 2) adding n-butanol saturated by water into the extract, and extracting; centrifuging by using a centrifuge, and collecting the extracted phase; and carrying out reduced-pressure concentration of the extracted phase by a rotary evaporator to obtain a brown-yellow extract; and 3) loading the brown-yellow extract on a macroporous resin column, then carrying out gradient elution with elution liquids according to an order from small elution liquid polarity to large elution liquid polarity, detecting, taking a fraction having the flavone content of more than 97%, concentrating to a dried powder, recrystallizing with methanol or ethanol, and thus obtaining a flavone product with the purity of more than 98%. The product has less impurity content and high biological activity, and the sub-critical fluid extraction technology has the advantages of high extraction efficiency, short operating period, energy saving, time saving, and high recovery rate of the extraction agent.
Description
Technical field
The invention belongs to Separation of Natural Products purification field, relate to the extracting method of biologically active substance flavones in Moringa, relate to sub critical extraction technology, is the method for biologically active substance in a kind of fine separation Moringa.
Background technology
Moringa (Moringa oleifera Lam), belongs to Capparales Moringaceae, claims again drumstick tree, tree etc. extremely, originates in the sub-Himalaya region of northern India and Africa, is extensively distributed in the state such as India, Pakistan.Moringa is nutritious, Moringa set contained calcareous be four times of milk, potassium is three times of banana, iron is three times of spinach, vitamin C is seven times of citrus, Vitamin A is four times of Radix Dauci Sativae.The nutritive substance such as several mineral materials, VITAMIN that Moringa blade, fruit pod contain, makes it have the function of Dietotherapy health as food consumption, also can be used for the aspects such as medicine, health care, industry, is described as " tree of mystery ", " Tree of Life ".Moringa tree is in recent years at the emerging a kind of health plant (food) of America and Europe, is known as high calcium, high protein, high fiber, low lipid, and has physical strength reinforcing, treatment anaemia, suppresses germ, drives away the effects such as parasite.
Flavonoid substances as the important class of biologically active substance, has played vital effect in edible, the pharmaceutical use of Moringa.Two of flavonoid compound general references have a series of compounds that the phenyl ring of phenolic hydroxyl group links mutually by central thricarbon atom, and its basic parent nucleus is 2-phenyl chromone.It is a kind of very strong oxidation inhibitor, can effectively remove the oxyradical in body, stops degeneration, the aging of cell, also can stop the generation of cancer.The method of extraction, isolating biologically active material from Moringa, now widely used is solvent-extraction process.This kind of method be Moringa blade drying and crushing, then with an organic solvent room temperature or heating and refluxing extraction, and concentrating under reduced pressure, obtains the concentrated extract of biologically active substance enrichment.Bioactive ingredients in traditional method for extracting natural product is the work that time consumption and energy consumption expends again solvent, also exist simultaneously and be extracted the shortcomings such as composition loss is large, the operational cycle is long, energy consumption is high, operation is inconvenient, extraction yield is not high, the needs of modern production can not be adapted to, particularly in the leaching process of modernization Chinese herbal medicine effective ingredients, these shortcomings day by day manifest, and have caused the significant wastage of human and material resources resources.
Subcritical fluids refer to some compound in temperature higher than its boiling point but lower than critical temperature, and under the condition of pressure lower than its emergent pressure, this material existing with fluid form.Subcritical fluids has wetting property and the diffustivity of approximate supercutical fluid, but there is no so harsh temperature and pressure requirement.Select suitable material, can under lesser temps and pressure, operate.These character are all conducive to extract biologically active substance from natural product.
Subcritical fluid extraction (Sub-critical Fluid Extraction) technology, to utilize subcritical fluids as extraction agent, in the pressurized vessel of airtight, anaerobic, low pressure, according to the similar principle mixing of organism, the diffusion in immersion process by material and extraction agent, reach target component diffusion transfer in solid materials in extraction agent, the process of passing through reduction vaporization again, by extraction agent and object product separation, finally obtains a kind of novel extraction and the isolation technique of object product.Nineteen thirty-nine, the Henry Rosenthal of the U.S. initiates the rudimentary gaseous alkanes liquefying after compression is leached for oil plant, becomes the blank of subcritical extraction method.
The method that uses subcritical fluid extraction technology to compare conventional solvent heating and refluxing extraction has the following advantages: extraction rate is fast, extraction efficiency is high, the ethanol reflux extraction that the institutes such as contrast Sun Ming swallow and Wang Yumei are used (extracts three times, each 1.5 hours time of extracting), subcritical fluid extraction technology can shorten extraction time more than 2 times; Solvent-oil ratio is few, and more than 97% solvent can recycling use; Steep cencentration is high, has greatly reduced the cost of post-processed; Extract temperature not high, being applicable to is that heat-sensitive material extracts; Continuous production is good, should be able to be suitable for the extensive consecutive production of modernization; Save energy, safe and reliable; Simple in structure, easy to operate.
The patent No. is that 200910094239.9 Chinese patent discloses and a kind ofly from Moringa seed, extracts and draw nurse method of protein, and the method is: moringa seeds powder and water are mixed, add enzyme, and water-bath 12-48 hour, after tune PH=7-9 value, centrifugal, get supernatant liquid; Again adjust after supernatant liquor PH=10-12, abandon supernatant liquor, go precipitation.After dry, be Moringa protein.The method is easy, easy to operate.But do not relate to the extraction of the biologically active substance such as flavones in Moringa.
Summary of the invention
The object of this invention is to provide a kind of flavonoid biologically active substance method in high efficiency extraction Moringa.Use the method for subcritical fluid extraction, simple to operate, can shorten extraction time, reduce solvent load, reduce energy consumption, can improve extract yield and purity simultaneously.
Technical scheme of the present invention is as follows:
The method of extracting Moringa flavones from leaf of Moringa, step is as follows:
1) by dry Moringa blade, pulverizing, pack in filter basket, drop in subcritical abstraction still, airtight extraction kettle, regulates interior vacuum tightness to the 0.06～0.1Mpa of extraction kettle; Then add organic extractant, regulate solid-to-liquid ratio 1:5～20 (g/ml), be warming up to extraction liquid in subcritical abstraction still and, in subcritical state, extract and reclaim solvent, obtain blackish green medicinal extract;
2) in blackish green medicinal extract, add the water saturated n-butanol extraction of using of 5 times of medicinal extract quality; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract;
3) by 2) macroporous resin column on gained brown color medicinal extract, then press the polarity of elutriant with elutriant ascending, gradient elution, detects, and gets flavones content and is greater than 97% cut and is concentrated into dry powder.
Can continue to obtain purity with methyl alcohol or ethyl alcohol recrystallization is more than 98% flavonoid product.
Described step 1) organic extractant methanol aqueous solution (v:v=7:3～10:0) or aqueous ethanolic solution (v:v=7:3～10:0) preferably.
Described step 1) extract and reclaim solvent preferably, extraction liquid in extraction kettle to be extracted 2～3 times, each extraction time is 0.5h～4h, each gained extraction liquid drops into solvent recuperation still, is decompressed to vacuum tightness 0.01～0.1Mpa recovery solvent.
Described subcritical abstraction equipment is subcritical abstraction suite of equipment.Preferably CBE-5L type subcritical abstraction still.
The preferred temperature of described subcritical abstraction still is 80～200 DEG C, and pressure is 108kPa～4048kPa.
Described drying means is preferably vacuum-drying or lyophilize.
Described macroporous resin is the macroporous resin such as 10～60 orders the D101 purifying, AB-8 or X-5 preferably.
Described elutriant is preferably pure water, anhydrous methanol, dehydrated alcohol, methanol aqueous solution or/and aqueous ethanolic solution, and gradient elution speed is 0.2～4BV/h.
Described Moringa blade is dry, pulverizing preferably to 100～700 order.
It is described that to press the polarity of elutriant with elutriant ascending, gradient elution, can adopt conventional following method: by the descending order of polarity, for water > anhydrous methanol > dehydrated alcohol, elutriant system is according to proportioning opposed polarity difference, for example 99% water and 1% methanol-eluted fractions liquid system polarity are greater than the polarity of 50% water and 50% methanol-eluted fractions liquid system, gradient elution: by the composition and ratio that to a certain degree constantly changes elutriant, to change elutriant polarity, detect, getting flavones content is greater than 97% cut and is concentrated into dry powder, obtaining purity with methyl alcohol or ethyl alcohol recrystallization is more than 98% flavonoid product.
The Flavonoid substances that subcritical fluid extraction technology of the present invention is extracted in Moringa has the following advantages:
1. the present invention, with respect to the method for the elementary extraction of routine, has related to the fine separation of biologically active substance in Moringa, is more conducive to give full play to the effect of biologically active substance in Moringa.
2. subcritical fluid extraction technology has short, energy-and time-economizing of operational cycle, the advantage such as easy and simple to handle, the ethanol reflux extraction that the institutes such as contrast Sun Ming swallow and Wang Yumei are used extracts three times, each 1.5 hours time of extracting, subcritical fluid extraction technology can shorten extraction time more than 2 times.
3. traditional extraction with aqueous solution, the alcohol heating reflux extraction of comparing, subcritical fluid extraction technology extract flavonoid and saponins material impurities content is few, extraction yield is high, contrast Chen Rui spoils and waits research, use the single extraction yield of traditional ethanol extraction method 1.101%, subcritical fluid extraction technology single extraction yield is up to 2.21%.
4. the compare extraction agent such as butane, dme of traditional subcritical fluid extraction utilization, the present invention uses ethanol or methyl alcohol to make extraction agent, can more effective extraction Moringa in the biologically active substance of alcohol dissolubility.
5. the high decomposes of effectively having avoided flavonoid and saponins material in Moringa of short, extraction efficiency of extraction time, has fully retained the physiologically active of biologically active substance.
6. methyl alcohol, ethanol make extraction agent, recycle and reuse extraction agent, carry out gradient elution and recrystallization, have reduced impurity introduction volume, efficiently utilize extraction agent, economize on resources.
Brief description of the drawings
Fig. 1: subcritical fluid extraction technology is extracted the Flavonoid substances operational flowchart in Moringa.
Fig. 2: methyl alcohol subcritical fluid extraction technology and conventional extraction, supercritical fluid extraction operational zone schematic diagram.
Fig. 3: ethanol subcritical fluid extraction technology and conventional extraction, supercritical fluid extraction operational zone schematic diagram.
Specific embodiment
Be below the specific embodiment of the present invention, described embodiment is for further describing the present invention, but the scope of protection of present invention is not limited to following embodiment.
Embodiment 1
Get leaf of Moringa 1kg, pulverized 700 mesh sieves, pack in filter frame, drop into CBE-5L type subcritical abstraction still, airtight extraction kettle, regulates the interior vacuum tightness of extraction kettle to 0.1Mpa, add methanol extraction agent, adjusting solid-to-liquid ratio is 1:20 (g:ml), regulates and extracts 200 DEG C of temperature, extract pressure 4.048MPa, extract 3 times, extract 4h at every turn, extracting solution drops into solvent recuperation still, be decompressed to vacuum tightness 0.1Mpa and reclaim solvent, vacuum-drying, obtains blackish green medicinal extract.To the water saturated n-butanol extraction of using that adds 5 times of amounts (in mass) in medicinal extract; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract.Get D101 macroporous resin column on this medicinal extract, ascending by polarity with ethanol-water elution liquid of volume ratio 50:50～99.9:0.1, gradient elution, detect, get the cut that flavones content is greater than 97%, be concentrated into dry powder, obtaining brass series products 26.7g purity for 4 times by recrystallizing methanol is 99.1%;
Embodiment 2
Get leaf of Moringa 0.9kg, pulverized 100 mesh sieves, pack in filter frame, drop into subcritical abstraction still, airtight extraction kettle, regulates the interior vacuum tightness of extraction kettle to 0.06Mpa, add alcohol extraction agent, adjusting solid-to-liquid ratio is 1:5 (g:ml), regulates and extracts 80 DEG C of temperature, extract pressure 0.108MPa, extract 2 times, extract 0.5h at every turn, extracting solution drops into solvent recuperation still, be decompressed to vacuum tightness 0.01Mpa and reclaim solvent, vacuum-drying, obtains blackish green medicinal extract.To the water saturated n-butanol extraction of using that adds 5 times of amounts (in mass) in medicinal extract; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract.Get AB-8 macroporous resin column on this medicinal extract, ascending by polarity with ethanol-water, the dehydrated alcohol elutriant of volume ratio 50:50～99.9:0.1, gradient elution, detect, get the cut that flavones content is greater than 97%, be concentrated into dry powder, obtaining brass series products 22.6g purity for 2 times by recrystallizing methanol is 98.1%;
Embodiment 3
Get leaf of Moringa 1.3kg, pulverized 500 mesh sieves, pack in filter frame, drop into subcritical abstraction still, airtight extraction kettle, regulates the interior vacuum tightness of extraction kettle to 0.08Mpa, add alcohol extraction agent, adjusting solid-to-liquid ratio is 1:10 (g:ml), regulates and extracts 100 DEG C of temperature, extract pressure 0.223MPa, extract 3 times, extract 2h at every turn, extracting solution drops into solvent recuperation still, is decompressed to vacuum tightness 0.1Mpa, reclaims solvent, vacuum-drying, obtains blackish green medicinal extract.To the water saturated n-butanol extraction of using that adds 5 times of amounts (in mass) in medicinal extract; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract.Get AB-8 macroporous resin column on this medicinal extract, ascending by polarity with Methanol-water, the anhydrous methanol elutriant of volume ratio 50:50～99.9:0.1, gradient elution, detect, get the cut that flavones content is greater than 97%, be concentrated into dry powder, obtaining brass series products 28.5g purity for 4 times by recrystallizing methanol is 99.1%;
Embodiment 4
Get leaf of Moringa 1.4kg, pulverized 300 mesh sieves, pack in filter frame, drop into subcritical abstraction still, airtight extraction kettle, regulates the interior vacuum tightness of extraction kettle to 0.09Mpa, add methanol extraction agent, adjusting solid-to-liquid ratio is 1:12 (g:ml), regulates and extracts 120 DEG C of temperature, extract pressure 0.642MPa, extract 3 times, extract 2.5h at every turn, extracting solution drops into solvent recuperation still, be decompressed to vacuum tightness 0.1Mpa and reclaim solvent, vacuum-drying, obtains blackish green medicinal extract.To the water saturated n-butanol extraction of using that adds 5 times of amounts (in mass) in medicinal extract; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract.Get the upper X-5 macroporous resin column of this medicinal extract 60g, with ethanol-water of volume ratio 50:50～99.9:0.1, dehydrated alcohol elutriant by the ascending gradient elution of polarity, detect, get the cut that flavones content is greater than 97%, be concentrated into dry powder, obtaining brass series products 31.3g purity for 3 times by recrystallizing methanol is 98.9%.
Claims (8)
1. from leaf of Moringa, extract the method for Moringa flavones, it is characterized in that step is as follows:
1) by dry Moringa blade, pulverizing, pack in filter basket, drop in subcritical abstraction still, airtight extraction kettle, regulates interior vacuum tightness to the 0.06～0.1Mpa of extraction kettle; Then add organic extractant, regulate solid-to-liquid ratio 1:5～20 (g/ml), be warming up to extraction liquid in subcritical abstraction still and, in subcritical state, extract and reclaim solvent, obtain blackish green medicinal extract;
2) in blackish green medicinal extract, add the water saturated n-butanol extraction of using of 5 times of medicinal extract quality; Use whizzer is centrifugal, collects extraction phase; By extraction phase with Rotary Evaporators in the time that vacuum tightness is 0.01～0.1Mpa, be evaporated to brown color medicinal extract;
3) by 2) macroporous resin column on gained brown color medicinal extract, then press the polarity of elutriant with elutriant ascending, gradient elution, detects, and gets flavones content and is greater than 97% cut and is concentrated into dry powder.
2. the method for claim 1, is characterized in that described step 1) organic extractant is methanol aqueous solution (v:v=7:3～10:0) or aqueous ethanolic solution (v:v=7:3～10:0).
3. the method for claim 1, it is characterized in that described step 1) extraction reclaims solvent and is, extraction liquid in extraction kettle is extracted 2～3 times, and each extraction time is 0.5h～4h, each gained extraction liquid drops into solvent recuperation still, is decompressed to vacuum tightness 0.01～0.1Mpa and reclaims solvent.
4. the method for claim 1, is characterized in that described subcritical abstraction equipment is subcritical abstraction suite of equipment.
5. the method as described in claim 1 or 4, is characterized in that described subcritical abstraction still temperature is 80～200 DEG C, and pressure is 108kPa～4048kPa.
6. the method for claim 1, is characterized in that described drying means is vacuum-drying or lyophilize.
7. the method for claim 1, is characterized in that described macroporous resin is the macroporous resin of 10～60 orders the D101 purifying, AB-8 or X-5.
8. the method for claim 1, is characterized in that described elutriant is pure water, anhydrous methanol, dehydrated alcohol, methanol aqueous solution or/and aqueous ethanolic solution, and gradient elution speed is 0.2～4BV/h.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN105623842A (en) * | 2015-12-23 | 2016-06-01 | 天津大学 | Method for subcritical extraction of moringa oleifera seed oil and method for testing location distribution of triglyceride in moringa oleifera seed oil |
| CN107569527A (en) * | 2017-10-31 | 2018-01-12 | 桂林纽泰生物科技有限公司 | The method that general flavone is extracted from hyperici,herba |
| CN107582593A (en) * | 2017-10-31 | 2018-01-16 | 桂林纽泰生物科技有限公司 | The method that general flavone is extracted from beggar-ticks |
| CN108771039A (en) * | 2018-07-02 | 2018-11-09 | 河南牧业经济学院 | chicken feed additive and preparation method thereof |
| CN108929201A (en) * | 2018-08-20 | 2018-12-04 | 凤阳县小岗村永和营养保健品有限公司 | A kind of method that Subcritical Water Extraction technology extracts cannabidiol |
| CN115138096A (en) * | 2022-07-07 | 2022-10-04 | 广州美林生态科技有限公司 | Method for extracting tea saponin by subcritical ethanol and/or methanol |
| CN116139186A (en) * | 2022-12-27 | 2023-05-23 | 中国科学院武汉植物园 | Subcritical preparation method of moringa oleifera leaf extract, and method and application of subcritical preparation method for moringa oleifera leaf extract to increase abundance of intestinal butyric acid |
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| CN107582582A (en) * | 2017-11-03 | 2018-01-16 | 隆安县荣胜养殖有限公司 | A kind of technique of extraction purification Moringa flavones |
-
2014
- 2014-04-29 CN CN201410178030.1A patent/CN103980242B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| 张灿等: "马兰黄酮类化合物的提取及其抗氧化活性", 《农业工程学报》, vol. 27, 31 December 2011 (2011-12-31), pages 307 - 311 * |
| 王俊儒: "《天然产物提取分离与鉴定技术》", 31 May 2006, article "天然产物提取分离与鉴定技术", pages: 6-8 * |
| 陈瑞娇等: "辣木叶总黄酮的提取及其降血糖的作用", 《食品与生物技术学报》, vol. 26, no. 4, 31 July 2007 (2007-07-31), pages 42 - 45 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104490954A (en) * | 2014-12-24 | 2015-04-08 | 广州泽力医药科技有限公司 | Method for extracting moringa oleifera |
| CN105623842A (en) * | 2015-12-23 | 2016-06-01 | 天津大学 | Method for subcritical extraction of moringa oleifera seed oil and method for testing location distribution of triglyceride in moringa oleifera seed oil |
| CN107569527A (en) * | 2017-10-31 | 2018-01-12 | 桂林纽泰生物科技有限公司 | The method that general flavone is extracted from hyperici,herba |
| CN107582593A (en) * | 2017-10-31 | 2018-01-16 | 桂林纽泰生物科技有限公司 | The method that general flavone is extracted from beggar-ticks |
| CN108771039A (en) * | 2018-07-02 | 2018-11-09 | 河南牧业经济学院 | chicken feed additive and preparation method thereof |
| CN108929201A (en) * | 2018-08-20 | 2018-12-04 | 凤阳县小岗村永和营养保健品有限公司 | A kind of method that Subcritical Water Extraction technology extracts cannabidiol |
| CN115138096A (en) * | 2022-07-07 | 2022-10-04 | 广州美林生态科技有限公司 | Method for extracting tea saponin by subcritical ethanol and/or methanol |
| CN116139186A (en) * | 2022-12-27 | 2023-05-23 | 中国科学院武汉植物园 | Subcritical preparation method of moringa oleifera leaf extract, and method and application of subcritical preparation method for moringa oleifera leaf extract to increase abundance of intestinal butyric acid |
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