A kind of device of preserving for a long time biological sample by gasification liquid nitrogen
Technical field
The present invention relates to a kind of biological sample storage device, particularly a kind of device of preserving for a long time biological sample by gasification liquid nitrogen.
Background technology
Liquid nitrogen, in bio-pharmaceuticals, is generally used as the cold medium of long-term preservation biological sample in medical and health industry.Traditional liquid nitrogen Techniques of preserving is positioned over biological sample in sealing liquid nitrogen container.Because liquid nitrogen can volatilize along with the time, regular check liquid nitrogen horizontal is the committed step in management.Along with a large amount of biological samples, as stem cell, start in a planned way to carry out long term storage.The low temperature Techniques of preserving of this original extensive style, had both been unfavorable for management on large, and especially numerical control management also increases keeping cost greatly, once and because of mismanagement, cause liquid nitrogen consumption, the biological sample loss consequence causing cannot make up.
Summary of the invention
The object of the invention is in order to overcome above deficiency, a kind of be applicable to extensive numerical control biological sample preservation and management use are provided, can not cause the device that gasification liquid nitrogen is preserved biological sample for a long time that passes through of liquid nitrogen waste.
Object of the present invention is achieved through the following technical solutions: a kind of device of preserving for a long time biological sample by gasification liquid nitrogen, comprise deep cooling storage bin, the top and the bottom of deep cooling storage bin are equipped with the first heat-insulation layer, in deep cooling storage bin, run through and be provided with central air jet pipe, between deep cooling storage bin and central air jet pipe, by rolling bearing, be connected, between deep cooling storage bin and central air jet pipe, form chilling chamber, in chilling chamber, be provided with temp probe, central authorities' air jet pipe is provided with puff prot, the two ends up and down of central authorities' air jet pipe are all placed in the outside of the first heat-insulation layer, one end that central authorities' air jet pipe is positioned at the first heat-insulation layer outside, top is liquid nitrogen import and is connected with control valve, one end that central authorities' air jet pipe is positioned at the first heat-insulation layer outside, bottom is nitrogen outlet and is connected with pressure-control valve.
Further improvement of the present invention is: described deep cooling storage bin comprises multilayer deep cooling box group, and every group of deep cooling box group includes a plurality of deep cooling boxes that arrange around central air jet pipe.
Further improvement of the present invention is: described in each deep cooling box comprise box body, with the deep cooling door of the second heat-insulation layer, between box body and deep cooling door, by rotating shaft, be connected, the back of deep cooling box is opened to chilling chamber, deep cooling door is outwards open.
Further improvement of the present invention is: it is characterized in that: described in each, the below of deep cooling box is equipped with dragging track.
The present invention compared with prior art has the following advantages: use more for convenience, can well guarantee the temperature of each deep cooling box, and be convenient to pick and place sample, facilitate extensive numerical control management, save production cost, avoided the loss of the biological sample that the consumption due to liquid nitrogen causes.
accompanying drawing explanation:
Fig. 1 is structural representation of the present invention;
Fig. 2 be in Fig. 1 A-A to structural representation;
Fig. 3 is the structural representation of deep cooling box;
Number in the figure: 1-deep cooling storage bin, 2-the first heat-insulation layer, 3-central authorities air jet pipe, 4-rolling bearing, 5-chilling chamber, 6-puff prot, 7-deep cooling box group, 8-deep cooling box, 9-box body, 10-the second heat-insulation layer, 11-deep cooling door, the import of 12-liquid nitrogen, 13-control valve, 14-nitrogen outlet, 15-pressure-control valve, 16-temp probe, 17-rotating shaft.
embodiment:
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, and this embodiment only, for explaining the present invention, does not form limiting the scope of the present invention.
As Fig. 1 to Fig. 3 shows a kind of embodiment of preserving for a long time the device of biological sample by gasification liquid nitrogen of the present invention, comprise deep cooling storage bin 1, the top and the bottom of deep cooling storage bin 1 are equipped with the first heat-insulation layer 2, in deep cooling storage bin 1, run through and be provided with central air jet pipe 3, between deep cooling storage bin 1 and central air jet pipe 3, by rolling bearing 4, be connected, between deep cooling storage bin 1 and central air jet pipe 3, form chilling chamber 5, in chilling chamber 5, be provided with temp probe 16, central authorities' air jet pipe 3 is provided with puff prot 6, the two ends up and down of central authorities' air jet pipe 3 are all placed in the outside of the first heat-insulation layer 2, one end that central authorities' air jet pipe 3 is positioned at the first heat-insulation layer 2 outsides, top is liquid nitrogen import 12 and is connected with control valve 13, one end that central authorities' air jet pipe 3 is positioned at the first heat-insulation layer 2 outsides, bottom is nitrogen outlet 14 and is connected with pressure-control valve 15, the preferred deep cooling storage bin 1 of the present invention comprises multilayer deep cooling box group 7, every group of deep cooling box group 7 includes a plurality of deep cooling boxes 8 that arrange around central air jet pipe 3, each deep cooling box 8 comprises box body 9, deep cooling door 11 with the second heat-insulation layer 10, between box body 8 and deep cooling door 11, by rotating shaft 17, be connected, the back of deep cooling box 8 is opened to chilling chamber 5, deep cooling door 11 is outwards open, the below of each deep cooling box 8 is equipped with dragging track.
Biological sample storage device of the present invention adopts continuous jet-propelled deep cooling store method to preserve, this device does not directly adopt liquid nitrogen to preserve, suitable storage temperature should be than ℃ slightly high 5-10 ℃ left and right, condensing temperature-196 of liquid nitrogen, its actual temp is arranged and is controlled by central control room, liquid nitrogen is sent into from the liquid nitrogen import 12 of central air jet pipe 3, the puff prot 6 spraying paint on pipe 3 by central authorities enters chilling chamber, gas is discharged by the nitrogen outlet 14 below pipeline after surpassing certain pressure, each deep cooling box back is open towards chilling chamber 5, to keep minimum temperature, deep cooling box below is provided with sliding rail, be convenient to pick and place sample, 11, deep cooling door can outwards be opened, to pick and place at any time sample, between central authorities' air jet pipe and deep cooling storage bin, there is rolling bearing 4 to connect, whole like this deep cooling storage bin can rotate around central air jet pipe 3, be convenient to pick and place sample, whole device is used more for convenience.