CN103966076A - Experimental box for purifying biological extracts - Google Patents
Experimental box for purifying biological extracts Download PDFInfo
- Publication number
- CN103966076A CN103966076A CN201410208213.3A CN201410208213A CN103966076A CN 103966076 A CN103966076 A CN 103966076A CN 201410208213 A CN201410208213 A CN 201410208213A CN 103966076 A CN103966076 A CN 103966076A
- Authority
- CN
- China
- Prior art keywords
- box body
- purifying biological
- box
- biological extract
- container cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to an experimental box for purifying biological extracts. The experimental box is characterized by comprising a strip-like box body which is provided with a plurality of accommodating chambers at intervals, wherein the opening of each accommodating chamber is located on the upper surface of the box body, a sealing film is formed on the upper surface of the box body, and the sealing film seals the opening of each accommodating chamber. The experimental box provided by the invention has the beneficial effects that an experimental sample is just added in use to be placed in an automatic nucleic acid extractor for a nucleic acid extraction experiment, automatic operation of the automatic nucleic acid extractor is fully utilized without continuously adding various reagents into the experimental process, so that the degree of automation is improved and the experimental operating time is saved, namely, the operating efficiency is improved. Meanwhile, pollution is reduced and the extraction rate and purity are improved. Connecting pieces and positioning pieces are arranged, so that the box body is stably fixed in the extractor for test. Meanwhile, the effect of identifying the accommodating chambers is further realized to avoid mis-addition of the reagents.
Description
Technical field
The present invention relates to a kind of experiment box, relate in particular to a kind of experiment box for purifying biological extract.
Background technology
Nucleic acid is one of base substance of life.Nucleic acid is extensively present in all animals, vegetable cell, microorganism, biological nucleic acid in vivo is normal and protein bound forms nucleoprotein.
Divide according to nucleic acid extraction principle: classical way has boiling lysis, phenol-chloroform extraction method.Novel method has silicon fiml absorption method, magnetic bead partition method etc.
Magnetic bead separation principle: magnetic bead is to wrap up the materials such as silicon-dioxide by the magnetic core surface with superparamagnetism to form, utilize these magnetic beads can realize the automated operation of nucleic acid extraction, magnetic bead has very strong avidity to nucleic acid, albumen under certain condition, and in the time that condition changes, the separate nucleic acid of magnetic bead and its absorption, reaches the object of nucleic acid extraction.Certainly, magnetic bead separation principle is also the separation that is applicable to protein, and therefore, it can be also protein that the object of experiment (extract) can make nucleic acid.
The step of magnetic bead partition method is: 1, cracking Lysis: discharge nucleic acid; 2, in conjunction with Bind: on the silica gel mould that is adsorbed onto magnetic bead exoperidium of nucleic acid specificity; 3, washing Wash: the magnet of magnetic bead quilt is fixed, and impurity is removed in washing; 4, wash-out Elute: nucleic acid is eluted from the silica gel mould of magnetic bead exoperidium by changing the conditions such as pH value, chaotropic salt.
At present main test kit be used for holding lysate, in conjunction with liquid, washings, and elutriant, and be to add respectively different solution to reach the object of nucleic acid extraction in the process of extracting.The most obvious feature of this test kit is need be at different experimentations, can add different reagent and just can reach experiment purpose.Therefore, the problem that this test kit exists: be to add respectively as required reagent in the process of experiment, complex operation, is unfavorable for automated operation; Affected by environment and working method, cause reagent contaminated, affect extraction effect.
Summary of the invention
The object of the invention is to solve the problems referred to above of prior art existence and a kind of experiment box for purifying biological extract is provided, the reagent of experiment use is sealed up for safekeeping in advance in box body, when experiment, opening sealer is putting box body in extraction apparatus, coordinate extraction apparatus to realize the extraction of nucleic acid or protein, reach that level of automation is high, operation efficiency is high, reduce the object of polluting, improving extraction yield and DNA purity.
Above-mentioned technical purpose of the present invention mainly solves by the following technical programs: a kind of experiment box for purifying biological extract, it is characterized in that comprising the box body that is strip, box body is provided with several spaced container cavities, described in each, the opening of container cavity is positioned at cartridge upper surface, described cartridge upper surface is provided with sealer, and described sealer seals the opening of container cavity described in each.Only in use adds, entering experiment sample can put into automatic instrument for extracting nucleic acid and carry out nucleic acid extraction experiment, make full use of the automated operation of automatic instrument for extracting nucleic acid, without constantly add all ingredients in experimentation, improve level of automation, save the experimental implementation time, also just improved operation efficiency, simultaneously, reduce and pollute, improve extraction yield and DNA purity.
As the further of technique scheme improved and supplemented, the present invention adopts following technical measures: described container cavity comprises at least one cracking sap cavity, a magnetic bead chamber, at least two washing cavities, a wash-out chamber.Corresponding reagent is housed respectively in these container cavities, and satisfied experiment is substantially required.
For fully cracking and fully washing, described container cavity comprises at least one cracking sap cavity for subsequent use, at least one washing cavities for subsequent use.
Between described cracking sap cavity for subsequent use and described magnetic bead chamber, be connected by the first connection piece, between described washing cavities for subsequent use and described wash-out chamber, be connected by the second connection piece.Being provided for of two connection pieces distinguished different container cavities, avoids adding wrong reagent, be also conducive to simultaneously with extraction apparatus on respective outer side edges position.
In order to improve washing effect, described washing cavities is four, is wherein salt washing lotion chamber near the washing cavities in described magnetic bead chamber.
In order to increase cavity volume, described cracking sap cavity bottom is cylindrical cavity, and top is cylindroid chamber, and top cross-sectional dimension is greater than lower cross section size.
Described in each, the low side of container cavity is conical, conical lower section on the one hand for extraction apparatus on respective outer side edges position, on the other hand with extraction apparatus on bar magnet cover, bar magnet (bar magnet cover bottom be corresponding taper shape, namely there is corresponding tapering) coordinate, reduce as far as possible dead angle, can extract comparatively thoroughly nucleic acid.
Between bottom centre's point position, described magnetic bead chamber and washing cavities bottom centre's point for subsequent use position, be provided with the 3rd connection piece, the end of described the 3rd connection piece, is along flushing with the low side of container cavity described in each, and described magnetic bead chamber bottom centre selects position and washing cavities for subsequent use bottom centre and selects position one spacer perpendicular to described the 3rd connection piece is set respectively, the end of described spacer, flushes with the 3rd connection piece along middle part, two ends have respectively lower extending part, i.e. convex form under the end forms respectively along the both sides at middle part.The 3rd connection piece and the setting of spacer and the structure of spacer, can make stable being fixed in extraction apparatus of box body test.
In order to improve the sealing reliability between sealer and container cavity, stop the possibility of empty envelope, described described in each on container cavity peristome along protruding in cartridge upper surface, form respectively each flange, described sealer is sealed on flange.
In order conveniently to pick and place box body, described cartridge upper surface container cavity periphery described in each forms epitaxy part.
The beneficial effect that the present invention has: 1, only enter experiment sample in use adds and can put into automatic instrument for extracting nucleic acid and carry out nucleic acid extraction experiment, make full use of the automated operation of automatic instrument for extracting nucleic acid, without constantly add all ingredients in experimentation, improve level of automation, save the experimental implementation time, also just improved operation efficiency, simultaneously, reduce and pollute, improve extraction yield and DNA purity.2, the setting of each connection piece and spacer, is conducive to make box body to be stably fixed in extraction apparatus and tests, and also arrives the effect of respectively holding strong Division identification simultaneously, avoids adding wrong reagent.3, described in each, the low side of container cavity is conical, conical lower section on the one hand for extraction apparatus on respective outer side edges position, on the other hand with extraction apparatus on bar magnet cover, bar magnet (bar magnet cover bottom be corresponding taper shape, namely there is corresponding tapering) coordinate, reduce as far as possible dead angle, can extract comparatively thoroughly nucleic acid.4, in order to improve the sealing reliability between sealer and container cavity, stop the possibility of empty envelope, each container cavity peristome has flange at cartridge upper surface.
Brief description of the drawings
Fig. 1 is the structural representation of sealer state of the present invention.
Fig. 2 is that the present invention throws off the structural representation after sealer.
Fig. 3 is the structural representation of looking up of Fig. 2.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.
Embodiment: a kind of experiment box for purifying biological extract, as Figure 1-3, it comprises the box body 1 that is strip, box body is provided with several spaced container cavities, described in each, the opening of container cavity is positioned at box body 1 upper surface, described box body 1 upper surface is provided with sealer 2, the opening of described sealer 2 sealings container cavity described in each.Describedly on container cavity peristome, form respectively each flange along protruding in box body 1 upper surface described in each, described sealer 2 is sealed on flange.Described box body 1 upper surface container cavity periphery described in each forms epitaxy part 13.Described in each, the low side of container cavity is conical.
The most basic configuration of container cavity comprises 5, one wash-out chambeies 6 of washing cavities, 4, at least two, 3, one magnetic bead chambeies of at least one cracking sap cavity.In order to reach washing effect washing cavities 5, to be four be to hand over good scheme, is wherein salt washing lotion chamber near the washing cavities in described magnetic bead chamber 4.As shown in Figure 1-Figure 3, also comprise that described container cavity comprises 7, one washing cavities 8 for subsequent use of a cracking sap cavity for subsequent use.Certainly it is also feasible having respectively two and above cracking sap cavity 7 for subsequent use washing cavities 8 for subsequent use.Described cracking sap cavity 3 bottoms are cylindrical cavity, and top is cylindroid chamber, and top cross-sectional dimension is greater than lower cross section size.
Between cracking sap cavity 7 for subsequent use and described magnetic bead chamber 4, be connected by the first connection piece 9, between washing cavities 8 for subsequent use and described wash-out chamber 6, be connected by the second connection piece 10.
Between 4 bottom centre's point positions, magnetic bead chamber and washing cavities for subsequent use 8 bottom centre's point positions, be provided with the 3rd connection piece 11, the end of described the 3rd connection piece 11, is along flushing with the low side of container cavity described in each, and position is selected by described magnetic bead chamber 4 bottom centre and washing cavities for subsequent use bottom 8 center position arrange respectively a spacer 12 perpendicular to described the 3rd connection piece 11, the end of described spacer 12, flushes with the 3rd connection piece 11 along middle part, two ends have respectively lower extending part, i.e. convex form under the end forms respectively along the both sides at middle part.
Supporting bar magnet cover and the bar magnet of configuration and container cavity on extraction apparatus.The bottom tapering of bar magnet cover and the tapering one of reagent strip to, facilitate fully contact bottom of bar magnet cover, extraction nucleic acid.
When test, carry out according to the following steps:
S1 application of sample: tear the sealer on box body, add sample, put into automatic instrument for extracting nucleic acid;
S2 cracking/combination: bar magnet cover inserts in the cracking sap cavity on box body, by automatic instrument for extracting nucleic acid, bar magnet being enclosed within lysate hole produces and vibrates, and then use sample fully to mix with lysate, reach the object of sample cracking, after cracking completes, bar magnet inserts bar magnet cover, then inserts together magnetic bead chamber with bar magnet cover, bar magnet absorption magnetic bead, and insert in cracking sap cavity with magnetic bead;
S3 salt is washed: in cracking sap cavity, and the nucleic acid after magnetic bead absorption cracking, and from cracking sap cavity out, then enter salt and wash and in chamber, carry out salt and wash;
S4 washing: salt is washed after end, bar magnet cover is washed chamber and is out sequentially entered in follow-up washing cavities and wash successively respectively from salt, and while washing in each washing cavities, bar magnet leaves bar magnet cover, bar magnet cover moves up and down, and vibration nucleic acid and washings wash fully.
S5 wash-out: after having washed, bar magnet bar magnet cover together inserts wash-out chamber with bar magnet cover and magnetic bead and carries out wash-out, and when wash-out, bar magnet leaves bar magnet cover, and bar magnet cover moves up and down, and the nucleic acid that vibrates is contacted fully with elutriant.After having vibrated, bar magnet enters bar magnet cover, and magnetic bead is taken back to magnetic bead chamber, and then bar magnet cover and bar magnet are got back to starting position.Nucleic acid is stayed in wash-out hole, reaches the object of nucleic acid extraction.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention.In the above-described embodiments, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. the experiment box for purifying biological extract, it is characterized in that comprising the box body (1) that is strip, box body is provided with several spaced container cavities, described in each, the opening of container cavity is positioned at box body (1) upper surface, described box body (1) upper surface is provided with sealer (2), and described sealer (2) seals the opening of container cavity described in each.
2. a kind of experiment box for purifying biological extract according to claim 1, is characterized in that described container cavity comprises at least one cracking sap cavity (3), a magnetic bead chamber (4), at least two washing cavities (5), a wash-out chamber (6).
3. a kind of experiment box for purifying biological extract according to claim 2, is characterized in that described container cavity comprises at least one cracking sap cavity for subsequent use (7), at least one washing cavities for subsequent use (8).
4. a kind of experiment box for purifying biological extract according to claim 3, it is characterized in that being connected by the first connection piece (9) between described cracking sap cavity for subsequent use (7) and described magnetic bead chamber (4), between described washing cavities for subsequent use (8) and described wash-out chamber (6), be connected by the second connection piece (10).
5. a kind of experiment box for purifying biological extract according to claim 4, is characterized in that described washing cavities (5) is four.
6. a kind of experiment box for purifying biological extract according to claim 5, is characterized in that described cracking sap cavity (3) bottom is cylindrical cavity, and top is cylindroid chamber, and top cross-sectional dimension is greater than lower cross section size.
7. according to a kind of experiment box for purifying biological extract described in claim 3-6 optional, it is characterized in that the low side of container cavity is conical described in each.
8. a kind of experiment box for purifying biological extract according to claim 7, it is characterized in that being provided with the 3rd connection piece (11) between bottom centre's point position, described magnetic bead chamber (4) and washing cavities for subsequent use (8) bottom centre's point position, the end of described the 3rd connection piece (11), is along flushing with the low side of container cavity described in each, and position is selected by described magnetic bead chamber (4) bottom centre and washing cavities for subsequent use bottom (8) center position arranges respectively a spacer (12) perpendicular to described the 3rd connection piece (11), the end of described spacer (12), flushes with the 3rd connection piece (11) along middle part, two ends have respectively lower extending part, i.e. convex form under the end forms respectively along the both sides at middle part.
9. a kind of experiment box for purifying biological extract according to claim 8, is characterized in that described on container cavity peristome, forming respectively each flange along protruding in box body (1) upper surface described in each, and described sealer (2) is sealed on flange.
10. a kind of experiment box for purifying biological extract according to claim 9, is characterized in that described box body (1) upper surface container cavity periphery described in each forms epitaxy part (13).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410208213.3A CN103966076A (en) | 2014-05-16 | 2014-05-16 | Experimental box for purifying biological extracts |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410208213.3A CN103966076A (en) | 2014-05-16 | 2014-05-16 | Experimental box for purifying biological extracts |
Publications (1)
Publication Number | Publication Date |
---|---|
CN103966076A true CN103966076A (en) | 2014-08-06 |
Family
ID=51236085
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410208213.3A Pending CN103966076A (en) | 2014-05-16 | 2014-05-16 | Experimental box for purifying biological extracts |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103966076A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
CN106047661A (en) * | 2016-07-06 | 2016-10-26 | 秦荣 | PCR (polymerase chain reaction) detection reagent container and PCR pretreatment system |
CN106399054A (en) * | 2016-11-22 | 2017-02-15 | 安图实验仪器(郑州)有限公司 | Reaction chamber used for in-vitro diagnosis device and with cucurbit-shaped opening |
CN117360960A (en) * | 2023-12-07 | 2024-01-09 | 苏州天隆生物科技有限公司 | Tube-connecting type consumable, preparation method and use method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011004653A1 (en) * | 2009-07-09 | 2011-01-13 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
EP2338600A1 (en) * | 2009-12-10 | 2011-06-29 | F. Hoffmann-La Roche AG | Process head positioning |
CN103695292A (en) * | 2007-07-13 | 2014-04-02 | 汉迪实验室公司 | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
CN203807462U (en) * | 2014-05-16 | 2014-09-03 | 杭州奥盛仪器有限公司 | Experimental box for purifying biological extract |
-
2014
- 2014-05-16 CN CN201410208213.3A patent/CN103966076A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103695292A (en) * | 2007-07-13 | 2014-04-02 | 汉迪实验室公司 | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
WO2011004653A1 (en) * | 2009-07-09 | 2011-01-13 | 凸版印刷株式会社 | Nucleic acid extraction kit, nucleic acid extraction method, and nucleic acid extraction apparatus |
EP2338600A1 (en) * | 2009-12-10 | 2011-06-29 | F. Hoffmann-La Roche AG | Process head positioning |
CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
CN203807462U (en) * | 2014-05-16 | 2014-09-03 | 杭州奥盛仪器有限公司 | Experimental box for purifying biological extract |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105733927A (en) * | 2016-04-11 | 2016-07-06 | 广州市达安医疗器械有限公司 | Consumable for nucleic acid extraction and multiple combined consumables |
CN106047661A (en) * | 2016-07-06 | 2016-10-26 | 秦荣 | PCR (polymerase chain reaction) detection reagent container and PCR pretreatment system |
CN106047661B (en) * | 2016-07-06 | 2020-03-24 | 上海宏石医疗科技有限公司 | PCR detection reagent container and PCR pretreatment system |
CN106399054A (en) * | 2016-11-22 | 2017-02-15 | 安图实验仪器(郑州)有限公司 | Reaction chamber used for in-vitro diagnosis device and with cucurbit-shaped opening |
CN106399054B (en) * | 2016-11-22 | 2019-01-04 | 安图实验仪器(郑州)有限公司 | For in-vitro diagnosis equipment and with the reaction warehouse of shaped open |
CN117360960A (en) * | 2023-12-07 | 2024-01-09 | 苏州天隆生物科技有限公司 | Tube-connecting type consumable, preparation method and use method thereof |
CN117360960B (en) * | 2023-12-07 | 2024-02-27 | 苏州天隆生物科技有限公司 | Tube-connecting type consumable, preparation method and use method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103966076A (en) | Experimental box for purifying biological extracts | |
CN106124282B (en) | A kind of method of lamination centrifugal filtration separation and Extraction excretion body | |
US8597878B2 (en) | Device and procedure for automated isolation and purification of nucleic acids from complex starting materials of the users choice | |
US10851368B2 (en) | Device and process for automated extraction of nucleic acids | |
AU2013360712B2 (en) | Reaction vessel for sample preparation | |
CN106754880A (en) | Urine Rapid nucleic acid extraction kit | |
CN203807462U (en) | Experimental box for purifying biological extract | |
CN208964916U (en) | A kind of magnetic beads for purifying instrument of enrichment method | |
US11161107B2 (en) | Dispersive pipette extraction system for purification of large biomolecules | |
HRP20201452T1 (en) | Isolation of nucleic acids | |
CN105733935A (en) | Preparation and detection reagent card case for nucleic acid or protein | |
CN110951725A (en) | One-step nucleic acid extraction process based on paramagnetic particle method | |
CN201255708Y (en) | Magnetic separation device for high pass bioanalysis separation | |
CN203355863U (en) | Magnetic separation device for separating biological material | |
CN204607994U (en) | A kind of nucleic acid rapid extraction device | |
CN105021685B (en) | A kind of shell of the gel electrophoresis pre-prepared colloid of anti-leakage | |
CN207516132U (en) | A kind of multi-functional Tip SPE of high throughput ultramicron | |
CN206279200U (en) | A kind of townhouse pipe and matching used waste collecting device | |
CN205074084U (en) | Modular small -size desktop formula magnetic separation device of high flux | |
CN113174317B (en) | Integrated upper opening nucleic acid quick-lifting test tube, quick-lifting detection device and method | |
CN204162719U (en) | A kind of reaction unit for ankyrin chip or gene chip | |
CN209438625U (en) | A kind of 96 orifice plate of modified | |
CN211235559U (en) | Card type micro test tube reaction cup | |
CN218811643U (en) | Be used for manual magnetic rod cover isolation frame that draws of 96 orifice plates nucleic acid | |
CN209568092U (en) | A kind of magnetic frame suitable for Sanger sequencing product purification |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C12 | Rejection of a patent application after its publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140806 |