CN103951762A - Extraction and separation method of extracellular scleroglucan - Google Patents
Extraction and separation method of extracellular scleroglucan Download PDFInfo
- Publication number
- CN103951762A CN103951762A CN201410222584.7A CN201410222584A CN103951762A CN 103951762 A CN103951762 A CN 103951762A CN 201410222584 A CN201410222584 A CN 201410222584A CN 103951762 A CN103951762 A CN 103951762A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- scleroglucan
- liquid
- pyrenomycetes
- extracellular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses an extraction and separation method of extracellular scleroglucan, belonging to the technical field of polysaccharide extraction and separation. The extraction and separation method comprises the steps of firstly, enabling a fermentation liquid to penetrate into a microfiltration membrane to remove mycelium, then, adding ammonium sulfate into the filtered clear liquid, magnetically stirring the liquid, standing and salting out, next, adding distilled water to enable polysaccharide to be suspended, dissolved and diluted, and transferring a dissolving liquid into a dialysis bag with the molecular weight of 100-200KDa to dialyze; then, collecting a polysaccharide liquid in the dialysis bag, regulating the pH value of the polysaccharide liquid to 7-9 by using a NaOH liquid, adding absolute ethyl alcohol, standing and carrying out alcohol precipitation to obtain extracellular scleroglucan; and finally, cleaning the extracellular scleroglucan by using a small deal of diethyl ether, then, decompressing, carrying out rotary evaporation and concentration, and carrying out freeze drying at a low temperature to obtain extracellular scleroglucan with high purity. By using the extraction and separation method, the extracellular scleroglucan with high purity is separated and extracted under the conditions of relatively low energy consumption, relatively little organic solvent and relatively low expense. The extraction and separation method has strong applicability and is an optimal method for separating and extracting the extracellular scleroglucan with high purity at present.
Description
Technical field
The invention belongs to polysaccharide extraction and separation technology field, be specifically related to the extraction and separation method of pyrenomycetes exocellular polysaccharide.
Background technology
Fungus polysaccharide is that a class of separating from the sporophore of fungi, mycelium and fungal fermented filtrate has extensive bioactive macromole carbohydrate.Research discovery, fungus polysaccharide as the aspect such as food, material is all with a wide range of applications, demonstrates the application prospect more more wide than animal and plant polysaccharide at many industrial circles.The physiological functions such as medical research shows, fungus polysaccharide has the immunizing power of raising, antitumor, hypoglycemic, reducing blood-fat, delay senility.
Scleroglucan (Scleroglucan) is the microbial polysaccharide by some filamentous fungus synthesis secretions of sclerotium (Sclerotium rolfsii), its structure is triple-helix structure, main chain is made up of β-D-(1 → 3)-glucopyranosyl unit, β-D-glucopyranosyl that every three unit have (1 → 6) key to connect
[1].In general, the average molecular mass of this polysaccharide is (1.4～5.4) × 10
6da
[2].
Scleroglucan is applied in oil production at first, and its effect is mainly reflected in following 3 aspects: lubricated drill bit, increase compared with the viscosity of thin oil slurry, and increase sea pressure, be convenient to oil production.Scleroglucan solution can withstand high temperatures, pH and the multiple ionogen of wider range, demonstrates than the better stability of xanthan gum in oil production
[3,4,5].
In recent years, scleroglucan good solubility, compatibility and rheological make it more and more be subject to food service industry person's favor.Scleroglucan has been applied in food as food thickener at present
[6].Research discovery, scleroglucan has the exocellular polysaccharide of anti-tumor activity to demonstrate better anti-tumor activity and anti-microbial activity than other
[7].
At present, the extracting method of scleroglucan be mainly by the fermented liquid high speed centrifugation after high temperature bath except mycelia, then alcohol precipitation obtains Crude polysaccharides, then removes albumen by Sevag method, activated carbon decolorizing, mistake chromatography column obtains the scleroglucan of higher degree
[8].Above method, not only complicated operation, complex steps, and cost is higher, and the instrument of use is expensive, easily exists organic reagent residual in polysaccharide, is not suitable for the higher scleroglucan of rapid, high volume production purity.
Summary of the invention
The invention provides that a kind of technique is simple, reliable, the low extracting method that can obtain again higher degree pyrenomycetes exocellular polysaccharide of cost.
Each medium component and compound method:
PDA slant medium (1L): potato 200g, glucose 20g, agar 20g, distilled water dissolves, natural pH.
Seed culture medium (1L): glucose 30.0g, NaNO
33.0g, yeast soaks powder 1.0g, K
2hPO
41.0g, MgSO
47H
2o0.25g, KCl0.5g, distilled water dissolves, and 1～3M HCl solution regulates pH to 4.5～5.0.
Fermention medium (1L): corn gluten (foodstuffs industry company limited of Huanglong of Jilin Province provides) 50mL, W-Gum 35g, K
2hPO
41.0g, MgSO
47H
2o0.25g, KCl0.5g, NaNO
33.0g, citric acid 0.4g, distilled water dissolves, and 1～3M HCl solution regulates pH to 4.5～5.0.
(1) actication of culture: pyrenomycetes (under 4 DEG C of conditions, paraffin is sealed up for safekeeping) is inoculated on the PDA slant medium of 121 DEG C of sterilizings to constant temperature culture 2～3d under 25～30 DEG C of conditions.
(2) seed culture fluid preparation: inject appropriate sterilized water in PDA slant medium, marginal not enters flushing inclined-plane, limit, obtains pyrenomycetes bacteria suspension; Bacteria suspension is accessed in the seed culture medium of 121 DEG C of sterilizings by 7～10% inoculum size, shake up; Be shaking culture 2～3d under 25～30 DEG C, the stirring velocity condition that is 180～230rmp in temperature.
(3) fermented liquid preparation: the inoculum size by 6～10% is to access seed culture fluid in the fermention medium of 121 DEG C of sterilizings, be that 25～30 DEG C, stir speed (S.S.) are that 180～230rmp, air flow are to cultivate 3～7d under 2～5L/min condition in temperature, obtain pyrenomycetes fermented liquid.
The extracting method of pyrenomycetes exocellular polysaccharide:
(1) microfiltration membrane in 0.45～1.2 μ m aperture is laid on micro-strainer screen, fermented liquid is poured in micro-strainer, sealing micro-strainer; The air compressor connecting by outside is exerted pressure (0.2～0.4MPa) to fermented liquid liquid level, collects and filters clear liquid;
(2) in the filtration clear liquid obtaining to step (1), add the ammonium sulfate that filters clear liquid quality 35～40%, stir ammonium sulfate is dissolved completely, under 3～8 DEG C of conditions, leave standstill the 8～12h that saltouts, outstanding on polysaccharide, reclaim(ed) sulfuric acid ammonium;
(3) get the polysaccharide after step (2) is saltoutd, the distilled water that adds 0.5～2 times of volume makes its dissolving, lysate is transferred in the dialysis tubing that molecular weight is 100～120KDa, sealing dialysis tubing, dialysis tubing is placed in to distilled water, 2～10 DEG C of dialysis 2～5d, thus remove the impurity such as ion in polysaccharide;
(4) collect the polysaccharide soln in dialysis tubing, by its pH regulator to 7～9, add dehydrated alcohol, making dehydrated alcohol volumetric concentration is 50～70%, under 2～10 DEG C of conditions, leave standstill alcohol precipitation 10～20h, gained precipitation with after water dissolution under 50～60 DEG C of conditions reduction vaporization concentrated;
(5) the concentrated solution frozen drying under-50～-40 DEG C of conditions step (4) being obtained, thus platinum sponge shape or Powdered pyrenomycetes exocellular polysaccharide obtained.
Pyrenomycetes of the present invention is Luo Eratai bacterium (Athelia roffsii) or Sclerotium rolfsii (Sclerotium rolfsii Sacc).
Advantage of the present invention is:
The present invention has realized the in the situation that of less energy consumption, less organic solvent, less cost, separation and Extraction high purity scleroglucan.This invention has very strong applicability, is the optimization method of current separation and Extraction high purity scleroglucan.
Brief description of the drawings
Fig. 1: the ultraviolet absorpting spectrum of the Luo Eratai bacterium exopolysaccharide solution that the embodiment of the present invention 1 obtains;
Luo Eratai bacterium exopolysaccharide ultraviolet absorpting spectrum shows that Luo Eratai bacterium exopolysaccharide has simple spike at 197nm place, and at 280nm place without the peak appearance of obviously mixing, show that the polysaccharide material purity that the present invention extracts is higher, without impurity such as albumen.Record in the polysaccharide sample of extraction Luo Eratai bacterium exopolysaccharide purity higher than 85% through anthrone-sulfuric acid process and fluorescent method.
Fig. 2: polysaccharide Standardization curve for fluorescence intensity figure.
Embodiment
Embodiment 1
Its step is as follows:
(1) obtain pyrenomycetes fermented liquid:
Each medium component and compound method:
PDA slant medium: potato 20g, glucose 2g, agar 2g, distilled water 100mL, natural pH.
Seed culture medium: glucose 9g, NaNO
30.9g, yeast soaks powder 0.3g, K
2hPO
40.3g, MgSO
47H
2o0.075g, KCl0.15g, with distilled water dissolving, is mixed with 300mL seed culture medium, with 2M HCl solution adjusting pH to 4.5.
Fermention medium: corn gluten 150mL, W-Gum 105g, K
2hPO
43.0g, MgSO
47H
2o0.75g, KCl1.5g, NaNO
39.0g, citric acid 1.2g, with distilled water dissolving, is mixed with 3L fermention medium, and 2M HCl solution regulates pH to 4.5.
(1) actication of culture: by the Luo Eratai bacterium that under 4 DEG C of conditions, paraffin is sealed up for safekeeping
[9](Athelia roffsii AY6657741 is stored in Jilin Agriculture University's Food Science and Engineering institute fermenting experiment chamber) is inoculated on the pipe of 3 after sterilizing PDA slant medium, constant temperature culture 2d under 29 DEG C of conditions.
(2) seed culture fluid preparation: inject some ml sterile waters in the PDA slant medium after activation, marginal not enters flushing inclined-plane, limit, obtains bacteria suspension, put into the seed culture medium through the 300mL of 121 DEG C of sterilizing 20min, shake up, 29 DEG C, 200rmp shaking culture 3d.
(3) fermented liquid preparation: add 3L fermention medium in 5L fermentor tank, 121 DEG C of sterilizing 20min, are cooled to 29 DEG C, add 270mL seed culture fluid, be 2L/min at 29 DEG C, air flow, under the condition of stir speed (S.S.) 180rmp, cultivate 3.5d, obtain Luo Eratai fermented liquid.
The extraction of Luo Eratai bacterium exopolysaccharide, its step is as follows:
(1) the mixed cellulose ester microfiltration membrane in 0.8 μ m aperture is laid on the screen of micro-strainer, fixing; Luo Eratai fermented liquid is poured in micro-strainer into sealing micro-strainer; The air compressor connecting by outside applies the normal atmosphere of 0.4Mpa to fermented liquid liquid level, make fermented liquid see through microfiltration membrane, removes mycelia, collects and filters clear liquid;
(2) filter to 1L the ammonium sulfate that adds 350g in clear liquid, stir ammonium sulfate is dissolved completely, under 4 DEG C of conditions, leave standstill the 12h that saltouts, outstanding on polysaccharide, Partial Protein sedimentation, reclaim(ed) sulfuric acid ammonium;
(3) get the upper outstanding polysaccharide after saltouing, the distilled water that adds 1 times of volume dissolves it, and lysate is transferred in the dialysis tubing that molecular weight is 100KDa, and sealing dialysis tubing, is placed in distilled water by dialysis tubing, and under 4 DEG C of conditions, dialysis 3d, changes water during this time 5 times.
(4) collect the Luo Eratai granulose solution in dialysis tubing, regulate its pH to 7.5 with the NaOH solution of 0.25M, add dehydrated alcohol, making dehydrated alcohol volumetric concentration is 70%, 4 DEG C of standing alcohol precipitation 12h, gained precipitation with after water dissolution under 50 DEG C of conditions reduction vaporization concentrated;
(5) concentrated solution is placed in to frozen drying under-50 DEG C of conditions, obtains platinum sponge shape Luo Eratai bacterium exopolysaccharide.
The mensuration of Luo Eratai bacterium exopolysaccharide purity, its step is as follows:
(1) drafting of Luo Eratai bacterium exopolysaccharide concentration-Standardization curve for fluorescence intensity
Compound concentration is the NaOH solution of 10.601g/L, and pH value is 13.5.Luo Eratai bacterium exopolysaccharide standard substance (purchased from Dextra company of the Britain) solution of the NaOH solution preparation 1mg/mL that is 13.5 by pH value.Under room temperature condition, magnetic agitation 0.5h.
Get respectively 0,0.5,1,1.5,2,2.5,3, the above-mentioned Luo Eratai bacterium exopolysaccharide standard solution of 3.5,4mL1mg/mL, supplies respectively 5mL with the NaOH solution (pH is 13.5) of 10.601g/L, being mixed with concentration is 0,0.1,0.2,0.3,0.4,0.5, the Luo Eratai bacterium exopolysaccharide standard solution of 0.6,0.7,0.8mg/mL.At excitation wavelength 365nm, emission wavelength 366nm place surveys the fluorescence intensity level of each concentration Luo Eratai bacterium exopolysaccharide solution.Taking polysaccharide soln concentration as X-coordinate, fluorescence intensity is ordinate zou, draws Luo Eratai bacterium exopolysaccharide concentration-Standardization curve for fluorescence intensity, sees Fig. 2.Obtain following fitting formula:
Y=31.155x (R
2=0.9990); Wherein,
Y-fluorescence intensity;
X-polysaccharide standard solution concentration, mg/mL.
(2) fermentation obtains the purity testing of Luo Eratai bacterium exopolysaccharide in polysaccharide sample.
Get the Luo Eratai bacterium exopolysaccharide that above-mentioned steps of the present invention is extracted, be mixed with the polysaccharide sample solution of 0.4mg/mL with the NaOH solution (pH is 13.5) of 10.601g/L, under room temperature condition, magnetic agitation 0.5h.At excitation wavelength 365nm, emission wavelength 366nm place surveys its fluorescence intensity level.According to fitting formula y=31.155x (R
2=0.9990) calculate the concentration of Luo Eratai bacterium exopolysaccharide in sample liquid glucose, thereby obtain the purity of sample liquid glucose Luo Eratai bacterium exopolysaccharide.
Recording Luo Eratai bacterium exopolysaccharide purity in this polysaccharide sample through fluorescent method is 85.41%.Ultraviolet full wavelength scanner finds that this polysaccharide has simple spike to occur at 197nm place, and without obviously assorted peak, illustrates that this purity of polysaccharide is higher.Again through anthrone-sulfuric acid process survey (known testing method) must this polysaccharide sample in Luo Eratai bacterium exopolysaccharide purity be 85.23%.
Reference
[1]G.Brigand:Scleroglucan.In:Industrial?Gums,Academic?Press,New?York,USA(1993)pp.461–472.
[2]J.I.Farina,F.Sineriz,O.E.Molina,N.I.Perotti,Isolation?and?physicochemical?characterization?of?soluble?scleroglucan?from?Sclerotium?rolfsii–Rheological?properties,molecular?weight?and?conformational?characteristics,Carbohydr.Polym.44(2001)41–50.
[3]B.McNeil,L.M.Harvey,Viscous?fermentation?products,Crit.Rev.Biotechnol.13(1993)275–304.
[4]S.A.Williams,Enhanced?oil?recovery.US?patent3,373,810(1968).
[5]G.Holzworth,Xanthan?and?scleroglucan:Structure?and?use?in?enhanced?oil?recovery,Dev.Ind.Microbiol.26(1985)271–280.
[6]S.A.SURVASE?et?al.Fermentative?Production?of?Scleroglucan[J].Food?Technol.Biotechnol,2007,45(2):107–118.
[7]P.P.Singh,R.L.Wisler,R.Tokuzen,W.Nakahara,Scleroglucan,an?antitumor?polysaccharide?from?Sclerotium?glucanicum,Carbohydr.Res.37(1974)245–247.
[8]Griffith?WL,Compere?A?L.Dev?Int?J?Biol?Micromol,1987；9:153-157.
[9] Jin Ping, Gao Xiaoyu. the research [J] of southern blight. agricultural pest research, 2011,1 (1): 14-22.
Claims (3)
1. an extracting method for pyrenomycetes exocellular polysaccharide, its step is as follows:
(1) microfiltration membrane in 0.45～1.2 μ m aperture is laid on micro-strainer screen, fermented liquid is poured in micro-strainer, sealing micro-strainer; The air compressor connecting by outside is exerted pressure to fermented liquid liquid level, collects and filters clear liquid;
(2) in the filtration clear liquid obtaining to step (1), add the ammonium sulfate that filters clear liquid quality 35～40%, stir ammonium sulfate is dissolved completely, under 3～8 DEG C of conditions, leave standstill the 8～12h that saltouts, outstanding on polysaccharide, reclaim(ed) sulfuric acid ammonium;
(3) get the polysaccharide after step (2) is saltoutd, the distilled water that adds 0.5～2 times of volume makes its dissolving, lysate is transferred in the dialysis tubing that molecular weight is 100～120KDa, sealing dialysis tubing, dialysis tubing is placed in to distilled water, 2～10 DEG C of dialysis 2～5d, thus remove the impurity such as ion in polysaccharide;
(4) collect the polysaccharide soln in dialysis tubing, by its pH regulator to 7～9, add dehydrated alcohol, making dehydrated alcohol volumetric concentration is 50～70%, under 2～10 DEG C of conditions, leave standstill alcohol precipitation 10～20h, gained precipitation with after water dissolution under 50～60 DEG C of conditions reduction vaporization concentrated;
(5) the concentrated solution frozen drying under-50～-40 DEG C of conditions step (4) being obtained, thus platinum sponge shape or Powdered pyrenomycetes exocellular polysaccharide obtained.
2. the extracting method of a kind of pyrenomycetes exocellular polysaccharide as claimed in claim 1, is characterized in that: pyrenomycetes fermented liquid is prepared by following steps,
(1) actication of culture: pyrenomycetes is inoculated on the PDA slant medium of 121 DEG C of sterilizings to constant temperature culture 2～3d under 25～30 DEG C of conditions.
(2) seed culture fluid preparation: inject appropriate sterilized water in PDA slant medium, marginal not enters flushing inclined-plane, limit, obtains pyrenomycetes bacteria suspension; Bacteria suspension is accessed in the seed culture medium of 121 DEG C of sterilizings by 7～10% inoculum size, shake up; Be shaking culture 2～3d under 25～30 DEG C, the stirring velocity condition that is 180～230rmp in temperature.
(3) fermented liquid preparation: the inoculum size by 6～10% is to access seed culture fluid in the fermention medium of 121 DEG C of sterilizings, be that 25～30 DEG C, stir speed (S.S.) are that 180～230rmp, air flow are to cultivate 3～7d under 2～5L/min condition in temperature, obtain pyrenomycetes fermented liquid.
3. the extracting method of a kind of pyrenomycetes exocellular polysaccharide as described in claim 1～2 any one, is characterized in that: pyrenomycetes is Luo Eratai bacterium (Athelia roffsii) or Sclerotium rolfsii (Sclerotium rolfsii Sacc).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410222584.7A CN103951762A (en) | 2014-05-23 | 2014-05-23 | Extraction and separation method of extracellular scleroglucan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410222584.7A CN103951762A (en) | 2014-05-23 | 2014-05-23 | Extraction and separation method of extracellular scleroglucan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103951762A true CN103951762A (en) | 2014-07-30 |
Family
ID=51329132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410222584.7A Pending CN103951762A (en) | 2014-05-23 | 2014-05-23 | Extraction and separation method of extracellular scleroglucan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103951762A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107501438A (en) * | 2017-08-30 | 2017-12-22 | 华熙福瑞达生物医药有限公司 | A kind of production method of Scleroglucan |
| CN109044876A (en) * | 2018-10-19 | 2018-12-21 | 天津科技大学 | A kind of water lock moisture-keeping composition rich in scleroglucan |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1454904A (en) * | 2003-05-08 | 2003-11-12 | 扬州大学 | Method of preparing high purity fungus polysaccharide |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN102336839A (en) * | 2011-09-30 | 2012-02-01 | 宁波餐之皇食品有限公司 | Method for extracting asteroid polysaccharide |
| CN102796205A (en) * | 2012-08-24 | 2012-11-28 | 黄河三角洲京博化工研究院有限公司 | Preparation method of high-transparency scleroglucan |
| CN103073652A (en) * | 2012-12-28 | 2013-05-01 | 昆明振华制药厂有限公司 | Method for extracting polysaccharide of spirulina platensis |
| CN103509130A (en) * | 2013-09-25 | 2014-01-15 | 江苏神华药业有限公司 | Novel green process for extracting ganoderma lucidum polysaccharides |
| CN103687600A (en) * | 2011-03-22 | 2014-03-26 | 印度血清研究所有限责任公司 | A novel process for preparation of polysaccharides |
-
2014
- 2014-05-23 CN CN201410222584.7A patent/CN103951762A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1454904A (en) * | 2003-05-08 | 2003-11-12 | 扬州大学 | Method of preparing high purity fungus polysaccharide |
| CN102154408A (en) * | 2011-01-13 | 2011-08-17 | 天津市工业微生物研究所 | Sclerotium rolfssii scleroglucan online fermentation extraction method and system |
| CN103687600A (en) * | 2011-03-22 | 2014-03-26 | 印度血清研究所有限责任公司 | A novel process for preparation of polysaccharides |
| CN102336839A (en) * | 2011-09-30 | 2012-02-01 | 宁波餐之皇食品有限公司 | Method for extracting asteroid polysaccharide |
| CN102796205A (en) * | 2012-08-24 | 2012-11-28 | 黄河三角洲京博化工研究院有限公司 | Preparation method of high-transparency scleroglucan |
| CN103073652A (en) * | 2012-12-28 | 2013-05-01 | 昆明振华制药厂有限公司 | Method for extracting polysaccharide of spirulina platensis |
| CN103509130A (en) * | 2013-09-25 | 2014-01-15 | 江苏神华药业有限公司 | Novel green process for extracting ganoderma lucidum polysaccharides |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107501438A (en) * | 2017-08-30 | 2017-12-22 | 华熙福瑞达生物医药有限公司 | A kind of production method of Scleroglucan |
| CN107501438B (en) * | 2017-08-30 | 2020-09-25 | 华熙生物科技股份有限公司 | Production method of sclerotium rolfsii gum |
| CN109044876A (en) * | 2018-10-19 | 2018-12-21 | 天津科技大学 | A kind of water lock moisture-keeping composition rich in scleroglucan |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101619300B (en) | Sphingomonas sp. TP-5 , method and application thereof for producing welan gum | |
| CN1313498C (en) | Process for producing pullulan | |
| CN105695543B (en) | A kind of production method of surfactin | |
| CN102876750B (en) | Method for extracting tremella polysaccharide and tremella protein | |
| CN107746433A (en) | A kind of preparation method of cellulose base antibacterial material dialdehyde cellulose lysine | |
| CN102242165A (en) | Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same | |
| CN103468611B (en) | The preparation method of fermention medium and preparation method and application, lactic acid bacterium glucan | |
| CN103554293B (en) | A kind of preparation method and its usage of active low-molecular amount fucosan | |
| CN102206616A (en) | Bacillus cereus fermentation method for producing phosphatidase C | |
| CN105420127B (en) | High-yield strain of high-molecular-weight pullulan and method for producing high-molecular-weight pullulan by using high-yield strain | |
| CN103951762A (en) | Extraction and separation method of extracellular scleroglucan | |
| CN102438632A (en) | Novel coprinus comatus and tremella mesenterica mushroom strains, products and extracts thereof and compositions comprising them | |
| CN104031109B (en) | A kind of method of fermentable purifying tea saponin | |
| CN111635924B (en) | Liquid fermentation method for increasing yield of ganoderma lucidum extracellular polysaccharide | |
| CN103014095B (en) | Use of MetarhiziumtaiiGYYA0601 strain in hyaluronic acid production | |
| CN104651284A (en) | Sphingomonas sp. T-3 and method for producing biological polysaccharide and poly-beta-hydroxy-butanoic acid by co-fermentation | |
| Tiwari et al. | Comprehensive review on recent trends and perspectives of natural exo-polysaccharides: Pioneering nano-biotechnological tools | |
| EP1283896B1 (en) | Method for obtaining a polysaccharide substantially free from whole cells and cell debris | |
| CN105499603A (en) | Method for biosynthesizing silver nanoparticle bacteriostat through cordyceps militaris extracting solution | |
| CN106754561B (en) | A kind of thermophilic salt rosiness Cook bacteria strain and its application | |
| CN104726515A (en) | Method for extracting bacterial exopolysaccharide rich in fucose | |
| TWI422682B (en) | The use of Bacillus sp. For the production of surfactants and extracellular polysaccharides | |
| CN109797107B (en) | Preparation method of fungus mycelium fine powder | |
| CN1483827A (en) | Method for preparing active pachyman | |
| CN1228449C (en) | Ganoderma mycellium antitumour water soluble neteropolysaccharide and its preparation method and use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140730 |