CN103936879A - Plant polysaccharide extraction method based on ultrasonic waves - Google Patents
Plant polysaccharide extraction method based on ultrasonic waves Download PDFInfo
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- CN103936879A CN103936879A CN201410182784.4A CN201410182784A CN103936879A CN 103936879 A CN103936879 A CN 103936879A CN 201410182784 A CN201410182784 A CN 201410182784A CN 103936879 A CN103936879 A CN 103936879A
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Abstract
The invention discloses a plant polysaccharide extraction method based on ultrasonic waves. A polysaccharide is extracted by adopting ultrasonic waves according to a material-liquid ratio of 1:10 at ultrasonic power of 400-800W for 30-50 minutes in a gradual increase manner. Compared with the prior art, the extraction is performed by adopting ultrasonic waves, so that the method has the characteristics of short extraction time, low energy consumption and high efficiency; and especially, a fine polysaccharide product can be directly obtained after the extraction without a separating step, so that the method has popularization value.
Description
Technical field
The present invention relates to a kind of plant isolation technique, relate in particular to a kind of based on hyperacoustic vegetable polysaccharides extracting method.
Background technology
Polysaccharide is the polymer being formed by connecting by monose, the 1950's, finds that successively some fungus polysaccharides and higher plant polysaccharide have obvious antitumor activity.Find again that recently many herbal polysaccharides also have hypoglycemic activity.Since the seventies, scientists discovery polysaccharide and saccharide complex are not only in vivo as energy resource and constituent material, and importantly it is present in all membrane structures, participates in the comings and goings of cell in biological phenomena.Polysaccharose substance is the important component part of all Living organisms, is extensively present in animal, plant and microorganism wall, is the important biomolecules of another class except nucleic acid and protein in organism.Scientific research has confirmed that glucide has many biological activitys, comprise antitumor, immune, hypoglycemic and antiviral etc., and to the almost non-toxic side effect of body.Herbal polysaccharide is because having enhancing body immunologic function and the antitumor pharmacological action such as hypoglycemic, and almost there is no toxicity and side effect, therefore causes domestic and international pharmacologist, biologist and chemists' concern.
And vegetable polysaccharides extracting method of the prior art adopts the methods such as water extraction, sour formulation, alkaline extraction, ultrafiltration process to extract mostly, but, the problem that existing extracting method all exists is that extraction rate is too slow, efficiency is low, after even extracting, also need the step separating, therefore, be difficult to promote, need a kind of new extracting method to be born.
Summary of the invention
It is a kind of based on hyperacoustic vegetable polysaccharides extracting method that object of the present invention is just to provide in order to address the above problem.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The present invention includes following steps:
(1) take plant material portion to be extracted;
(2) be that 1:10 adds distilled water in the ratio of raw material and distilled water;
(3) adopt the ultrasonic extraction 30min that power is 400W;
(4) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 60%, if be less than 60%, to adopt power be that the ultrasonic wave 5min of 400W measures again again, until polysaccharide content enters next step after being greater than 60%;
(5) adopt the ultrasonic extraction 10min that power is 600W;
(6) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 80%, if be less than 80%, to adopt power be that the ultrasonic wave 5min of 600W measures again again, until polysaccharide content enters next step after being greater than 80%;
(7) adopt the ultrasonic extraction 10min that power is 800W;
(8) carry out the assay of polysaccharide, extract and finish if polysaccharide content is greater than 90%, obtain polysaccharide products, if be less than 90%, to adopt power be that the ultrasonic wave 5min of 800W measures again again, until polysaccharide content finishes after being greater than 90% to extract.
Beneficial effect of the present invention is:
The present invention is a kind of based on hyperacoustic vegetable polysaccharides extracting method, compared with prior art, the present invention adopts ultrasonic extraction, have the advantages that extraction time is short, energy consumption is low, efficiency is high, especially the present invention can directly obtain polysaccharide products after extraction, without the value of carrying out separating step, having applying
Embodiment
The invention will be further described below:
The present invention includes following steps:
(1) take plant material portion to be extracted;
(2) be that 1:10 adds distilled water in the ratio of raw material and distilled water;
(3) adopt the ultrasonic extraction 30min that power is 400W;
(4) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 60%, if be less than 60%, to adopt power be that the ultrasonic wave 5min of 400W measures again again, until polysaccharide content enters next step after being greater than 60%;
(5) adopt the ultrasonic extraction 10min that power is 600W;
(6) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 80%, if be less than 80%, to adopt power be that the ultrasonic wave 5min of 600W measures again again, until polysaccharide content enters next step after being greater than 80%;
(7) adopt the ultrasonic extraction 10min that power is 800W;
(8) carry out the assay of polysaccharide, extract and finish if polysaccharide content is greater than 90%, obtain polysaccharide products, if be less than 90%, to adopt power be that the ultrasonic wave 5min of 800W measures again again, until polysaccharide content finishes after being greater than 90% to extract.
The cardinal principle of ultrasonic extraction is: ultrasonic wave produces " cavatition ", this cavatition can produce local High Temperature High Pressure, and form powerful shockwave or high-speed jet, this powerful high-speed jet can reduce effectively, block layer between elimination and water, has strengthened mass-transfer efficiency.Meanwhile, high-speed jet produces a kind of physics shearing force to plant cell tissue, makes it distortion, breaks and discharge inclusion, and this has just accelerated extraction process greatly.In addition, hyperacoustic many second-order effects as heat effect, dissolve, spread, smash, chemical effect, biological effect, agglomeration effect etc. also can accelerate the diffusion of effective ingredients in plant in solvent and discharge, promote effective ingredients in plant and solvent, be conducive to extraction.
Embodiment 1: adopt above step to carry out polysaccharide extraction to pumpkin, along with the increase of hyperacoustic use power, the output of extracting pumpkin total reducing sugar, reducing sugar and the polysaccharide of gained also improves thereupon, and temperature and ultrasonic wave can act synergistically, and jointly improve the extraction yield of squash polyoses.Experimental result measure ultrasonic 50min polysaccharide yield and content all the highest.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (1)
1. based on a hyperacoustic vegetable polysaccharides extracting method, it is characterized in that, comprise the following steps:
(1) take plant material portion to be extracted;
(2) be that 1:10 adds distilled water in the ratio of raw material and distilled water;
(3) adopt the ultrasonic extraction 30min that power is 400W;
(4) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 60%, if be less than 60%, to adopt power be that the ultrasonic wave 5min of 400W measures again again, until polysaccharide content enters next step after being greater than 60%;
(5) adopt the ultrasonic extraction 10min that power is 600W;
(6) carry out the assay of polysaccharide, carry out next step if polysaccharide content is greater than 80%, if be less than 80%, to adopt power be that the ultrasonic wave 5min of 600W measures again again, until polysaccharide content enters next step after being greater than 80%;
(7) adopt the ultrasonic extraction 10min that power is 800W;
(8) carry out the assay of polysaccharide, extract and finish if polysaccharide content is greater than 90%, obtain polysaccharide products, if be less than 90%, to adopt power be that the ultrasonic wave 5min of 800W measures again again, until polysaccharide content finishes after being greater than 90% to extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410182784.4A CN103936879A (en) | 2014-04-30 | 2014-04-30 | Plant polysaccharide extraction method based on ultrasonic waves |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410182784.4A CN103936879A (en) | 2014-04-30 | 2014-04-30 | Plant polysaccharide extraction method based on ultrasonic waves |
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| Publication Number | Publication Date |
|---|---|
| CN103936879A true CN103936879A (en) | 2014-07-23 |
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| CN201410182784.4A Pending CN103936879A (en) | 2014-04-30 | 2014-04-30 | Plant polysaccharide extraction method based on ultrasonic waves |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560957A (en) * | 2022-04-12 | 2022-05-31 | 湖南明舜制药有限公司 | Method for extracting polygonatum odoratum polysaccharide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936294A (en) * | 2012-12-10 | 2013-02-20 | 青岛海尔软件有限公司 | Extraction method of kumquat polysaccharides |
| CN103694369A (en) * | 2013-12-30 | 2014-04-02 | 福建省神六保健食品有限公司 | Method for preparing lucid ganoderma polysaccharides |
| CN103724444A (en) * | 2013-12-20 | 2014-04-16 | 潍坊医学院 | Ultrasonic extraction method of Acanthopanax giraldii polysaccharide |
-
2014
- 2014-04-30 CN CN201410182784.4A patent/CN103936879A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936294A (en) * | 2012-12-10 | 2013-02-20 | 青岛海尔软件有限公司 | Extraction method of kumquat polysaccharides |
| CN103724444A (en) * | 2013-12-20 | 2014-04-16 | 潍坊医学院 | Ultrasonic extraction method of Acanthopanax giraldii polysaccharide |
| CN103694369A (en) * | 2013-12-30 | 2014-04-02 | 福建省神六保健食品有限公司 | Method for preparing lucid ganoderma polysaccharides |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560957A (en) * | 2022-04-12 | 2022-05-31 | 湖南明舜制药有限公司 | Method for extracting polygonatum odoratum polysaccharide |
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Application publication date: 20140723 |
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