CN103933085B - The application of Herba Andrographitis and phosphonomycin in the compound medicine for preparing treatment livestock and poultry coli-infection disease - Google Patents
The application of Herba Andrographitis and phosphonomycin in the compound medicine for preparing treatment livestock and poultry coli-infection disease Download PDFInfo
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- CN103933085B CN103933085B CN201410170961.7A CN201410170961A CN103933085B CN 103933085 B CN103933085 B CN 103933085B CN 201410170961 A CN201410170961 A CN 201410170961A CN 103933085 B CN103933085 B CN 103933085B
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- herba andrographitis
- phosphonomycin
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Abstract
The present invention relates to a kind of method that utilization Herba Andrographitis and phosphonomycin treat livestock and poultry coli-infection disease, by Herba Andrographitis and phosphonomycin according to 10 170：3 30 part by weight mixing, obtains the compound medicine containing Herba Andrographitis, the compound medicine is added in animal and fowl fodder according to the addition of 0.1 7g/kg feeds and fed, continuous to use 37 days.The present invention can reverse bacterial to the drug resistance of antimicrobial, so as to improve the therapeutic effect of antimicrobial, drug cost and medicament residue is greatly reduced, is that raiser creates higher economic benefit, and ensure the food security of the mankind.
Description
Technical field
Herba Andrographitis and phosphonomycin are utilized the invention belongs to livestock and poultry coli-infection treatment technology field, more particularly to one kind
The method for treating livestock and poultry coli-infection disease.
Background technology
Escherichia coli are common conditioned pathogens, are also a kind of disease often sent out, and it primary can cause the acute sepsis of chicken
The multiple location severe infections such as disease, peritonitis, hepatitis, pneumonia, enteritis, also can it is secondary or it is mixed send out in virosis, caused to aquaculture
Huge economic loss.Amoxicillin, ampicillin, Ceftiofur, ceftriaxone, amoxicillin/clavulanate, ammonia benzyl west
The beta-lactam nucleus class antibiotic such as woods/Sulbactam is the conventional important drugs for preventing and treating the disease, with the cultivation intensive journey of scale
Degree is more and more big, and medication is more and more spread unchecked, and its antibody-resistant bacterium is more and more more, and the report on Drug Resistance of E. coli is also more.
Research confirms that one of main mechanism of resistance is to produce beta-lactamase, especially extended spectrumβ-lactamase (Extended-
Spectrum β-lactamase, ESBLs), the enzyme can be relatively strong and rapidly hydrolyzes beta-lactam nucleus, loses this kind of antibiotic
Antibacterial activity.ESBLs is the main enzyme type of beta-lactamase, and it is not only to cephalo three generations and AZT resistance, and to ammonia
Base glycoside, quinolones and sulfamido are in crossing drug resistant, only to Carbapenems, a mould alkenes medicaments insensitive.Many documents
It has been reported that the multi-drug resistant of Escherichia coli has substantial connection with producing ESBLs, it is more paid close attention to both at home and abroad.Inventor is most
Nearly detection, extraction, enzyme percent hydrolysis and Sulbactam, the Tazobactam Sodium to antibiotic for having carried out food animal source pathogenic bacteria ESBLs
And the research such as the suppression enzyme protection effect of Chinese medicine, the drug sensitive test to part ESBLs Escherichia coli shows that its multidrug resistant rate is bright
It is aobvious to be higher than non-ESBLs bacterial strains.In addition, result also shows that production ESBLs bacterial strains are not only tight to third generation cephalosporins such as Ceftiofurs
Weight resistance, and also to a variety of antimicrobial resistances such as fluoroquinolones, aminoglycoside and sulfamido, phosphonomycin, in serious
Multidrug resistant.Inventor is separated to the Escherichia coli that resistance to phosphonomycin produces fosA3 drug resistant genes in production ESBLs bacterial strains, more strengthens
The drug resistance of bacterium.The drug resistance of Escherichia coli causes the increase of raiser's drug cost, treatment course extension, mortality of livestock
Increase, brings larger economic loss, and increase livestock and poultry medicament residue risk to production.Therefore, one kind is needed badly in actual production
Pair regardless of whether the Escherichia coli of resistance there can be a method of preferable therapeutic action, and treatment course can be shortened, reduce the death rate,
Economic loss and reduction medicament residue are reduced, is that raiser creates higher economic benefit, also ensures the health and safety of the mankind.
The content of the invention
Herba Andrographitis and phosphonomycin treatment livestock and poultry Escherichia coli sense are utilized the technical problem to be solved in the present invention is to provide one kind
Contaminate the method for disease, it can kill bacterium quickly, overcome weak curative effect that antimicrobial treatment caused due to drug resistance problems,
Consumption is big, residual is high and the high defect of cost, it is to avoid simple Chinese medicine sterilizing spectrum is narrow, the problem of sterilize slow.
To solve above technical problem, the present invention uses following technical scheme：One kind is treated using Herba Andrographitis and phosphonomycin
The method of livestock and poultry coli-infection disease, by Herba Andrographitis and phosphonomycin according to 10-170：3-30 part by weight mixing, is obtained
Compound medicine containing Herba Andrographitis, the compound medicine is added in animal and fowl fodder according to the addition of 0.1-7g/kg feeds and carried out
Feeding, it is continuous to use 3-7 days.
Described compound medicine is prepared into powder, tablet, oral liquid or granule.
The preparation method of described compound medicine is：Herba Andrographitis was crushed into 60 mesh sieves, then it is mixed with powdered antimicrobial
It is even, powder, tablet, oral liquid or granule are prepared into according to a conventional method.
The drug resistance problems existed for current livestock and poultry coli-infection, inventor utilizes Guangxi Special Traditional Chinese Medicine Herba Andrographitis
Compound medicine is constituted with antimicrobial.Research has shown that, is added in antimicrobial after Herba Andrographitis, can reverse bacterial to the resistance of antimicrobial
Property, so as to improve the therapeutic effect of antimicrobial.Powder, tablet, oral liquid or particle is made in the compound medicine according to a conventional method
Agent, is added in animal and fowl fodder according to the addition of 0.1-7g main ingredients/kg feeds and is fed, can be greatly reduced drug cost and
Medicament residue, is that raiser creates higher economic benefit, while also ensure that food security.
Embodiment
First, drug resistance inversion Effect study of the Herba Andrographitis to antimicrobial
Antimicrobial has the effect for the treatment of Escherichia coli in itself, but because the Escherichia coli for producing ESBLs drug resistant genes have
Stronger drug resistance, causes antimicrobial not show preferable therapeutic effect in the case of normal dosage.
In order to verify that Herba Andrographitis is acted on the drug resistance inversion of antimicrobial, microtest tube doubling dilution is used on 96 orifice plates
Method, first determines minimal inhibitory concentration and Herba Andrographitis pair of the antimicrobial to the e. coli strains of production ESBLs and fosA3 drug resistant genes
The subinhibitory concentration of ESBLs and fosA3 drug resistant gene e. coli strains is produced, wearing for subinhibitory concentration is then added in the medium
Heart lotus reuses the minimal inhibitory concentration of microtest tube doubling dilution culture assay antimicrobial；Added using continuous passage method
Have in the culture medium of same subinhibitory concentration Herba Andrographitis and same concentration antimicrobial to production ESBLs and fosA3 drug resistant gene large intestines
Bacillus strain carries out Secondary Culture, until the antimicrobial minimal inhibitory concentration observed diminishes more than 4 times compared with before passage, you can
It is judged as that Herba Andrographitis has drug resistance inversion effect to antibacterial medical instrument.Result of the test statistics is as shown in table 1：
The passage of the subinhibitory concentration of table 1 (0.25g/mL) Herba Andrographitis causes production ESBLs and fosA3 Escherichia coli Western medicine MIC's
Change.
As seen from the above table, antimicrobial reduces more than 4 times to the minimal inhibitory concentration of antibiotic-resistance E. coli, you can judgement is
Herba Andrographitis has drug resistance inversion effect to production ESBLs and fosA3 drug resistant gene Escherichia coli.
2nd, the application example of compound medicine of the present invention
Embodiment 1- embodiments 4 are prepared in accordance with the following methods：Herba Andrographitis was crushed after 60 mesh sieves, based on raw material,
It is put into blender and mixes with powdered antimicrobial ratio by weight again, according to a conventional method, compound medicine is prepared into
Agent product.
The embodiment 1-4 of table 2 formula and preparation formulation
| Numbering | Antimicrobial | Herba Andrographitis | Formulation |
| Embodiment 1 | Phosphonomycin 18g | 130g | Powder |
| Embodiment 2 | Phosphonomycin 3g | 50g | Tablet |
| Embodiment 3 | Phosphonomycin 9g | 10g | Oral liquid |
| Embodiment 4 | Phosphonomycin 30g | 170g | Granule |
Inventor is by the compound medicine product obtained by above-described embodiment 1-4, and the meat duck diarrhoea to 35 ages in days carries out curative effect reality
Test.
Clinical symptoms：Duck group, which is drawn in watery stools, excrement, obvious indigested feed.Suffer from diarrhoea individually serious duck by
Appearance pin gradually is soft slowly dead.
Cut open inspection lesion：The duck that dies of illness dehydration is than more serious, and indivedual ducks are still very big, duck liver enlargement, hyperemia, the liver of part duck
There are white films on dirty, air bag is muddy, tentative diagnosis is Escherichia coli or duck oromeningitis.
Test therapeutic regimen：Fall ill duck group's point six groups of administrations, first group with addition of the embodiment 1 according to 1g/kg feeds
Administration, second group is administered with embodiment 2 according to the addition of 2g/kg feeds, and the 3rd group with embodiment 3 according to 2g/kg feeds
Addition is administered, and the 4th group is administered with embodiment 4 according to the addition of 1g/kg feeds；5th group with phosphonomycin pulvis according to
The addition administration of 0.1g/kg feeds, the 6th group is administered with the thick powder of Herba Andrographitis according to the addition of 7g/kg feeds；The above six
Group therapeutic regimen is persistently used 5 days.
Using effect is fed back：After first group to the 4th group medication 3 days, sick duck no longer draws watery stools, formed stools, no longer
There is dead duck, be continuing with 2 days, duck group is almost recovered；After 5th group of medication 3 days, duck group's DeGrain is many or draw water
Sample excrement, containing indigested feed, is continuing with 2 days, somewhat takes a turn for the better, but without well thoroughly, dilute watery stools are still
Occur, raiser uses the compound medicine of the gained of embodiment 1 instead, is continuing with 3 days, excrement all switchs to normally；6th group of medication
After 3 days, duck is continuing with 2 days without too big remarkable improvement, and the duck phenomena of mortality are still without being effectively controlled.It can be seen that, contain
The curative effect that the compound medicine of Herba Andrographitis is suffered from diarrhoea to duck is better than folk prescription.
Claims (2)
1. Herba Andrographitis and phosphonomycin are according to part by weight 10-170：3-30 is used to prepare treatment livestock and poultry coli-infection disease
The application of compound medicine.
2. application according to claim 1, it is characterised in that described compound medicine is prepared into powder, tablet, oral liquid
Or granule.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410170961.7A CN103933085B (en) | 2014-04-25 | 2014-04-25 | The application of Herba Andrographitis and phosphonomycin in the compound medicine for preparing treatment livestock and poultry coli-infection disease |
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| CN201410170961.7A CN103933085B (en) | 2014-04-25 | 2014-04-25 | The application of Herba Andrographitis and phosphonomycin in the compound medicine for preparing treatment livestock and poultry coli-infection disease |
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| CN104435040A (en) * | 2014-11-07 | 2015-03-25 | 广西大学 | Balsamiferou blumea herb and fosfomycin containing compound medicine for livestock and poultry |
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Non-Patent Citations (2)
| Title |
|---|
| 毛皮动物消化***、呼吸***、泌尿***疾病及脑炎常用药物问答;闫新华;《特种经济动植物》;20070531;第19页 * |
| 鸡致病性大肠杆菌的耐药性分析试验;孔宪宏 等;《中国畜禽种业》;20120331;第152-154页 * |
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Inventor after: Si Hongbin Inventor after: Xu Suping Inventor after: Pan Jie Inventor after: Fu Yunhe Inventor after: Liao Rong Inventor after: Jiang Deshu Inventor after: Huang Kai Inventor after: Xia Juan Inventor after: Wang Qiuhua Inventor after: Liu Zengyuan Inventor after: Song Jianwu Inventor before: Si Hongbin Inventor before: Huang Kai Inventor before: Xia Juan Inventor before: Wang Qiuhua Inventor before: Liu Zengyuan Inventor before: Song Jianwu Inventor before: Xu Suping Inventor before: Pan Jie |
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