CN103931672A - Antibacterial sterilizing skin disinfectant liquid and antibacterial sterilizing antiseptic disinfector and preparation methods thereof - Google Patents
Antibacterial sterilizing skin disinfectant liquid and antibacterial sterilizing antiseptic disinfector and preparation methods thereof Download PDFInfo
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Abstract
The invention discloses an antibacterial sterilizing skin disinfectant liquid and an antibacterial sterilizing antiseptic disinfector and preparation methods thereof. The antibacterial sterilizing skin disinfectant liquid is mainly prepared from Bo-chrysanthemum, honeysuckle, senecio scandens, dandelion, ethanol, chlorhexidine, mint, and p-chloro dimethylphenol according to a certain weight proportion; and the skin disinfectant liquid has the functions of clearing heat and removing toxicity, diminishing inflammation and sterilizing, has strong disinfection antibacterial effects on skin and sanitation, and is good in sterilization effect.
Description
Technical field
The present invention relates to a kind of disinfectant, the definite disinfectant liquid for skin that says a kind of antibiotic and sterilizing and corrosion-proof and sterilization agent and preparation method thereof, belong to the field of Chinese medicines.
Background technology
Disinfectant liquid for skin and corrosion-proof and sterilization agent refer to for killing the pathogenic microorganism on communication media, make it reach the preparation of innoxious requirement, its Main Function in diseases prevention is that pathogenic microorganism is eliminated outside human body, cuts off the route of transmission of infectious disease, reaches the object of controlling infectious disease.The generation of the outburst of SARS epidemic situation, H5N1 avian flu cases in recent years, Sichuan violent earthquake in 2008 and novel influenza A virus in 2009 popular, has shifted disinfectant industry onto the prostatitis of epidemic prevention front again.Microorganism pathogenic in daily life is seen everywhere, and on these common article of coin, mobile phone, toilet, floor etc., pathogenic bacteria are ubiquitous.On current market, disinfectant application is very extensive, not only at medical field, also for our life.Along with people's health and the constantly enhancing of health care consciousness, people also constantly strengthen the demand of disinfectant in daily life.At present, the main effectively sterilization component that is mainly used in common objects surface class disinfectant on domestic and international market is to chloroxylenol, but the chemical composition in this disinfectant is to human body and article, not only there are certain excitant and corrosivity, and the physiological health of human body is also produced to certain negative effect.
Summary of the invention
Therefore, people produce great demand to the disinfectant liquid for skin of the Chinese drug preparation having no side effect and corrosion-proof and sterilization agent, up to now, also do not find about relevant report of the present invention, the inventor is through a large amount of development test trial-production, finally successfully develop the disinfectant liquid for skin of being prepared by Chinese herbal medicine, thereby completed the present invention.
Object of the present invention is just to provide a kind of disinfectant liquid for skin by Chinese drug preparation and corrosion-proof and sterilization agent.
Another object of the present invention has just been to provide the preparation method of this disinfectant liquid for skin and corrosion-proof and sterilization agent.
The scheme of disinfectant liquid for skin of the present invention combines preparation by bo-chrysanthemum, honeysuckle, climbing groundsel, dandelion, the fruit of summer cypress, peppermint, ethanol, chlorohexidene, by these material combination, make each medicine mutually produce synergy, thereby can reach effectively fast the effect of epidemic prevention, sterilization, bo-chrysanthemum wherein: < < China voluminous dictionary > > carries: feverfew main product Boxian, claim Bo chrysanthemum, performance optimal; < < Chinese medicinal herbal > > claim bo-chrysanthemum performance optimal in medicine chrysanthemum; 20th century Chinese medicine monumental work < < China herbal > > also claim that Bo chrysanthemum is best in quality, selects bo-chrysanthemum to be because its nature and flavor sweetness and bitterness is cool, enter lung, Liver Channel, has dispelling wind, the heat that disappears, improving eyesight, the function of removing toxic substances, control headache, dizzy, hot eyes, ambition dysphoria with smothery sensation, furunculosis, pyogenic infections.Select honeysuckle to be because its nature and flavor are sweet, cold, enter lung, stomach warp, there is heat-clearing, the function of removing toxic substances, controls warm disease heating, toxic-heat and blood stasis, large carbuncle, pyogenic infections, scrofula, anal fistula.Select climbing groundsel to be because its nature and flavor are bitter, cold, return lung, liver, large intestine channel, have clearing heat and detoxicating, improving eyesight, antipruritic function, controls anemopyretic cold, red eye, swell pain, dysentery, skin eczema, sore furuncle.Selecting dandelion is because its property bitter but sweet flavor, cold, enters liver, stomach warp, has clearing heat and detoxicatingly, and the function of diuresis dissipating bind, controls acute mastitis, lymphadenitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.Select the fruit of summer cypress to be because its nature and flavor sweetness and bitterness is cold, enter kidney, urinary bladder channel, have diuresis, the function of clearing away damp-heat, controls difficult urination, gonorrhoea, and under band, hernia, rubella, sore, mange, private parts is wet itches.Select peppermint to be because its nature and flavor are pungent, cool, enter lung, Liver Channel, have dispelling wind, heat radiation, wards off dirtyly, and the function of removing toxic substances, controls affection of exogenous wind-heat, headache, and hot eyes, abscess of throat, eats stagnant inflatable, aphtha, toothache, sore scabies, urticaria.Ethanol, English name ethyl alcohol; Ethanol, the skeleton symbol of ethanol is C
2h
5oH, is commonly called as alcohol, and it is a kind of inflammable, volatile colourless transparent liquid under normal temperature, normal pressure, and its aqueous solution has special, pleasant fragrance, and slightly with irritating.Having many uses of ethanol, it is a kind of good solvent, can dissolve many inorganic matters, can dissolve many organic matters again, so conventional ethanol dissolves plant pigment or medicinal ingredient wherein, also conventional ethanol, as the solvent of reaction, all can dissolve organic matter and the inorganic matter of participating in reaction, enlarge active surface, improves reaction rate.Chlorohexidene, chlorohexidene is a kind of broad-spectrum antibiotic, has great broad-spectrum antibacterial, bactericidal action, it is a kind of good sterilizing medicine, antibacterial action to Gram-positive and negative bacterium, stronger than disinfectants such as bromogeramines, even still effective when having serum, blood etc. to exist.
The amounts of components of raw material of the present invention is also that inventor carries out a large amount of Test Summaries and draws, the consumption of each component all has good effect within the scope of following weight.
Bo-chrysanthemum 550-650 kilogram of honeysuckle 65-75 kilogram of climbing groundsel 65-75 kilogram
Dandelion 65-75 kilogram of fruit of summer cypress 65-75 kilogram of peppermint 65-75 kilogram
Ethanol 50-100 kilogram of chlorohexidene 35-45 kilogram.
Preferable amount is
70 kilograms of 70 kilograms of climbing groundsels of 600 kilograms of honeysuckles of bo-chrysanthemum
70 kilograms of 70 kilograms of peppermints of 70 kilograms of fruit of summer cypress of dandelion
40 kilograms of 60 kilograms of chlorohexidenes of ethanol.
Embodiment
One, get the raw materials ready
1, bo-chrysanthemum: choose clean leaf stalk, anthocaulus and spare impurity;
2, honeysuckle: sieve removes silt, chooses clean impurity;
3, climbing groundsel: get crude drug, remove impurity, clean, slightly dry in the air, segment, dry;
4, dandelion: choose decontamination, clean earth, segment, dries;
5, the fruit of summer cypress: get crude drug, remove impurity, sieve removes chip;
6, peppermint: choose clean impurity, remove undesirable root, first leaf is trembled and separately put down, then stem is sprayed to clear water, run through rear segment, dry, then with leaf and even;
7, chlorohexidene: extract according to quantity;
8, ethanol: extract according to quantity.
Two, preparation
1, the bo-chrysanthemum of described weight, honeysuckle, climbing groundsel, dandelion, the fruit of summer cypress, peppermint are put into the container of boiling medicine, decoct twice, add for the first time 8 times of amounts of water, soak 30 minutes, 80-120 ℃ decocts 1.5 hours, uses inorganic ceramic membrane separation for removing impurities, obtains decoction liquor for the first time.
2, the dregs of a decoction of filtration are added to 4 times of amounts of water, 80-120 ℃ decocts 1 hour, uses hollow fiber ultrafiltration membrane separating and filtering, obtains decoction liquor for the second time.
3, merge twice decoction liquor, cooling at static 20-30 minute below 40 ℃, 200 eye mesh screens filter twice, get supernatant, then with nanofiltration and reverse osmosis membrane dehydration, concentrate extraction active component, obtain Chinese medical extract.
4, the chlorohexidene of above-mentioned weight is added in alcohol solvent and heats 60 ℃, stir, obtain liquor hibitane.
5, the liquor hibitane after dissolving is added to mixing and stirring in traditional Chinese medicine extract, obtain disinfectant liquid for skin.
Thimerosal of the present invention has removing toxic substances, anti-inflammatory, and the function of sterilization, strong for skin and disinfecting antibacterial action, bactericidal anti-inflammatory effect is good.
The integrated membrane separation process of described disinfectant liquid for skin, that Inorganic Ceramic Membranes is organically combined with hollow fiber ultrafiltration membrane isolation technics, nanofiltration and reverse osmosis membrane dehydration concentration technique, be combined into Integrated Films separation and extraction technology, reasonably use the advantage of different film separation remove the non-medicinal impurity in liquid and the dehydration of liquid is concentrated.Utilize inorganic ceramic membrane to carry out after effective separation and purification Chinese medical extract, can filtering protein, starch, pectin, tannin macromolecular substances and microorganism, reduce the loss of aqueous soluble active constituent, and Effective Component of Chinese Medicine is substantially without holding back, adopt inorganic ceramic membrane first liquid to be carried out to micro-filtration to remove the impurity such as tannin, starch, replace one time alcohol precipitation, then utilize the filter of hollow fiber ultrafiltration membrane essence, recycling nanofiltration and reverse osmosis membrane dewater concentrated, can realize without alcoholization and producing.Thereby substituted traditional water extraction and alcohol precipitation method technique, saved alcohol and energy consumption, technique is simple, personnel save, shorten the production cycle, and can effective guarantee drug quality.Integrated Films isolation technics is applied to Chinese medicine production and has significant economy and environment benefit.
Compare with traditional extraction technique, the inorganic ceramic membrane of take has following superiority as basic integrated membrane separation process:
(1) membrane process process does not change, and without heating, has saved energy utilization rate;
(2) in membrane process process, reduce the consumption of ethanol or replace ethanol a large amount of in former alcohol precipitation process, pollution-free, noresidue, increases the safety of producing, and is a kind of green environmental protection technique;
(3) adopt inorganic ceramic membrane to add the stepped new membrane separation-extraction technology of hollow-fibre membrane and can make full use of the corrosion-resistant easy cleaning of performance ceramic membrane and the high advantage of hollow-fibre membrane filtering accuracy, form optimum Match, stable;
(4) new membrane process is directly processed Chinese medical extract, effectively removes large molecule, tannin and other non-medical substance in liquid, has improved the content of liquid effective ingredient, makes the yield of product and quality obtain significant raising; Utilize nanofiltration and reverse osmosis membrane separation technology to replace the distillation of conventional multi, save alcohol and energy consumption, technique is simple, personnel save, with short production cycle, efficiency is high;
(5) filter process only adopts pressure as the power of film separation, so separator is simple, flow process is short, easy and simple to handle, technological parameter is easy to control;
(6) membrane separation technique has changed liquid after alcohol precipitation to the processing of liquid often stickiness is larger, and more difficult concentrated problem, guarantees that liquid preparation is during preservation not easy to produce precipitation or wall sticking phenomenon.
The present invention tests and makes after deliberation with the basic prescription of Huatuo < < Huatuo's Zhongzang classic > >, and put into practice in conjunction with modern Chinese medicine, merge modern Chinese medicine technology and refined the high-tech natural disinfectant product forming, called after hundred million people pacify board Chinese medicine environmental protection disinfectant, this formula is marrow " whole existing thought the with theory of traditional Chinese medical science, dialectical unify " for instructing, select genunie medicinal materials, accurate consumption, with medical scholar clinical practice invaluable experience over the past thousands of years, take basic prescription as basis, with science, actual effect, component principle is compatible accurately, have and meet objective guiding theory, sound and reliable basic Cheng Fang and the organic combination principle of system.According to the thimerosal of this advanced technology preparation, fungi mould, Candida albicans, staphylococcus aureus, Escherichia coli, virus and hospital's common pathogen can be killed.
When to skin degerming, what people worried most is exactly safety problem, so will attach great importance to this point when preparation sterile products.Hundred million people pacify disinfectant liquid for skin strict control requirement when preparation, the chlorohexidene of the honeysuckle that contains clearing heat and detoxicating composition, Bo chrysanthemum and the peppermint that contains fungicide active ingredient, the fruit of summer cypress etc. and effective sterilization and anticorrosion composition is cooperated, can effectively strengthen synergy, people be used not only safe but also effective.
Corrosion-proof and sterilization agent is selected climbing groundsel, honeysuckle, chrysanthemum, peppermint, ethanol, chloroxylenol raw material is combined, and by these material medicine combinations, makes each material medicine mutually produce synergy, thereby can reach effectively fast disinfection corrosion-resistant effect, wherein select climbing groundsel to be because its nature and flavor are bitter, cold, return lung, liver, large intestine channel, have clearing heat and detoxicating, improving eyesight, antipruritic function, control anemopyretic cold, red eye, swell pain, dysentery, skin eczema, sore furuncle.Select honeysuckle to be because its nature and flavor are sweet, cold, enter lung, stomach warp, there is heat-clearing, the function of removing toxic substances, controls warm disease heating, toxic-heat and blood stasis, large carbuncle, pyogenic infections, scrofula, anal fistula.Select chrysanthemum to be because its nature and flavor sweetness and bitterness is cool, enter lung, Liver Channel, has dispelling wind, the heat that disappears, and improving eyesight, the function of removing toxic substances, controls headache, dizzy, hot eyes, ambition dysphoria with smothery sensation, furunculosis, pyogenic infections.Select peppermint to be because its nature and flavor are pungent, cool, enter lung, Liver Channel, have dispelling wind, heat radiation, wards off dirtyly, and the function of removing toxic substances, controls affection of exogenous wind-heat, headache, and hot eyes, abscess of throat, eats stagnant inflatable, aphtha, toothache, sore scabies, urticaria.Ethanol, English name ethyl alcohol; Ethanol, the skeleton symbol of ethanol is C2H5OH, is commonly called as alcohol, and it is a kind of inflammable, volatile colourless transparent liquid under normal temperature, normal pressure, and its aqueous solution has special, pleasant fragrance, and slightly with irritating.Having many uses of ethanol, it is a kind of good solvent, can dissolve many inorganic matters, can dissolve many organic matters again, so conventional ethanol dissolves plant pigment or medicinal ingredient wherein, also conventional ethanol, as the solvent of reaction, all can dissolve organic matter and the inorganic matter of participating in reaction, enlarge active surface, improves reaction rate.To chloroxylenol, chemical name: CDMP, PCMX has shown unique sterilization and anti-corrosive properties combination, making can efficient and avirulent playing a role in many germicidal applications.It can be added in some medicinal pulvis, and soap, in amenities and anti-dandruff shampoo.PCMX also can be used as the glue that preservative and mildewcide are used, paint, and weaving, paper industry, in cosmetics and polishing agent, is efficient, safety, the bactericide of wide spectrum.General service: 1, PCMX has shown unique sterilization and the combination of anti-corrosive properties, making can efficient and avirulent playing a role in many germicidal applications.2, it can be added in some medicinal pulvis, and soap, in amenities and anti-dandruff shampoo.3, PCMX also can be used as the glue that preservative and mildewcide are used, paint, and weaving, paper industry, in cosmetics and polishing agent.
The consumption of raw material components of the present invention is also that inventor draws through repeatedly groping to sum up, and the consumption of each component all has good effect within the scope of following weight.
Climbing groundsel 20-30kg honeysuckle 20-30kg chrysanthemum 20-30kg
Peppermint 20-30kg ethanol 15-25kg is to chloroxylenol 0.4-0.6kg.
Preferable amount is
Climbing groundsel 25kg honeysuckle 25kg chrysanthemum 25kg
Peppermint 25kg ethanol 20kg is to chloroxylenol 0.5kg.
Embodiment
Preparation process of the present invention is as follows
One, get the raw materials ready
1, climbing groundsel: get crude drug, remove impurity, clean, slightly dry in the air, segment, dry;
2, honeysuckle: sieve removes silt, chooses clean impurity;
3, chrysanthemum: choose clean leaf stalk, anthocaulus and spare impurity;
4, peppermint: choose clean impurity, remove undesirable root, first leaf is trembled and separately put down, then stem is sprayed to clear water, run through rear segment, dry, then with leaf and even;
5, ethanol: extract according to quantity;
6, to chloroxylenol: extract according to quantity.
Two, preparation
1, by the climbing groundsel of described weight, honeysuckle, chrysanthemum, peppermint boiling secondary, add for the first time 8 times of amounts of water, soak after 30 minutes, 80-120 ℃ decocts 1.5 hours, uses inorganic ceramic membrane separation for removing impurities, obtains decoction liquor for the first time.
2, the dregs of a decoction of filtration are added to 6 times of amounts of water, 80-120 ℃ decocts 1 hour, uses hollow fiber ultrafiltration membrane separating and filtering, obtains decoction liquor for the second time.
3, merge twice decoction liquor, cooling at static 20-30 minute below 40 ℃, 200 mesh sieves filter secondary, get supernatant, then with nanofiltration and reverse osmosis membrane dehydration, concentrate extraction active component, obtain Chinese medical extract.
4, by described weight chloroxylenol is dissolved in the ethanol of described weight, stir 10 minutes, obtain chloroxylenol liquid.
5, will add in traditional Chinese medicine extract chloroxylenol liquid, mixing and stirring, obtains corrosion-proof and sterilization agent.
The application of corrosion-proof and sterilization agent in disinfectant products:
Escherichia coli, staphylococcus, false pseudomonas bacillus, candida albicans, trichophyton gypseum etc. are all had to good killing or inhibitory action, therefore can be used as disinfectant.
1, drip dew
As daily disinfectant, easy to use, rather well received.Corrosion-proof and sterilization agent consumption in dripping dew is 4.8-5.0%.
2, agent for air disinfection and refreshing
As daily air sanitizer, easy to use, client's favor.In this product, consumption is 0.5-1.0%.
In addition, can act on skin, otch, wound, the scratch agent of hospital's urological department, gynaecology, obstetrics and surgery, to skin nonirritant.Consumption as disinfectant is 5.0%.
In addition, also can be used as preservative and mould inhibitor for industrial circles such as glue, coating, paint, weaving, leather, papermaking.
Described inorganic ceramic membrane, is a kind of inorganic or macromolecular material with special Selective Separation function, and two parts that it can become not communicate fluid partitioning, see through one or more substances wherein, and by other separating substances out.The characteristics such as membrane separation technique is efficient with it, energy-saving and environmental protection and molecular level filtration, have been widely used in the fields such as medicine, water treatment, chemical industry, electronics, food processing, become one of most important technology in separation science in this century.Being acknowledged as the 21 century membrane technology of one of great industrial technology, is a kind of emerging green industry science and technology.Inorganic Ceramic Membranes is the sieve effect based on porous ceramics medium and the separating substances technology of carrying out, distinct dynamically " cross-flow filtration " modes of filter type such as employing and tradition " dead-end filtration " " cake filtration ": under pressure-driven, material liquid inside film pipe film surface with certain flow velocity flow at high speed, small-molecule substance (liquid) edge with it vertical direction sees through microporous barrier, macromolecular substances (or solid particle) tunicle is held back, and makes fluid reach the object of separated concentrated and purifying.Described hollow fiber ultrafiltration membrane is a kind of of milipore filter.It is a kind of technology the most ripe with advanced in hyperfiltration technique.Doughnut external diameter: 0.5-2.0nm, internal diameter: 0.3-1.4nm, is covered with micropore on doughnut tube wall, and express can hold back the molecular weight of material in aperture, and molecular cut off can reach several thousand to hundreds of thousands.Former water, in doughnut outside or inner chamber pressurized flow, forms respectively external-compression type and inner pressed.Ultrafiltration is dynamic filtration process, and being trapped material can get rid of with condensed water, and unlikely obstruction film surface, can move for a long time continuously.Milipore filter is one of polymeric membrane for separation of developing the earliest.Hyperfiltration technique is a kind of water purification that is widely used in, and solution is separated, concentrated, and recovers useful materials from waste water, the new and high technology in purification of waste water recycling field.Feature is that use procedure is simple, does not need heating, energy conservation, and low pressure operation, plant area area is little.Described nanofiltration, nanofiltration (NF, Nanofiltration) is a kind of pressure drive membrane separation process between reverse osmosis and ultrafiltration, the pore diameter range of NF membrane is in several nanometers left and right.Compare with other pressure-driven type film separation processes, occur more late.Its appearance can be traced back to the research of the NS-300 film of J.E.Cadotte at the end of the seventies, and afterwards, nanofiltration develops very soon, and film group device is in the mid-80 commercialization.NF membrane is mostly from reverse osmosis membrane derivation, as CA, CTA film, aromatic polyamide composite membrane and sulfonated polyether sulfone film etc.But compare with reverse osmosis, its operating pressure is lower, so nanofiltration is known as again " low pressure reverse osmosis " or " loose reverse osmosis " (Loose RO).Described reverse osmosis membrane, reverse osmosis membrane is the core parts of realizing reverse osmosis, is a kind of artificial semipermeable membrane with certain characteristic that biological semipermeable membrane is made of simulating.Generally with macromolecular material, make.As CAM, aromatic series polyhydrazide film, aromatic polyamide film.The diameter of surface micropore is generally between 0.5～10nm, and the size of permeability is relevant with the chemical constitution of film itself.Some macromolecular materials are good to the repellency of salt, and the penetration speed of water bad.Some high polymer material chemical structures have more hydrophilic radical, thereby the penetration speed of water is relatively very fast.Therefore a kind of reverse osmosis membrane of satisfaction should have suitable infiltration capacity or salt rejection rate.Reverse osmosis membrane should have following characteristics: (1) should have efficient salt rejection rate under high flow rate; (2) there is higher mechanical strength and service life; (3) can bring into play function compared with under low operating pressure; (4) can tolerate the impact of chemistry or biochemical action; (5) be subject to the factors such as pH value, temperature to affect less; (6) masking raw material sources are easy, process easy, with low cost.
By test example, further set forth below the beneficial effect of disinfectant liquid for skin of the present invention and corrosion-proof and sterilization agent.
Disinfectant liquid for skin of the present invention is called hundred million people in the following text and is pacified disinfectant liquid for skin and the rotten disinfectant of hundred million people's security protections and point and the comparative trial report of health skin antiseptic, the clean hand sanitizing fluid of poem:
Put and health disinfectant liquid for skin (Shanghai Linkwell Disinfection High-Tech Co Ltd's production), active ingredient is the povidone iodine containing available iodine 5000 m g/L; The clean hand sanitizing fluid of poem (the clean Co., Ltd in clean Kangbao County, Hangzhou produces), active ingredient is l0 L chlorhexidine acetate.Experimental observation four kinds of thimerosals antibody-resistant bacterium killing effect to hospital clinical separation, and compare with part of standards bacterial strain.
[test example]
1.1 test organisms sources
Clinical isolates strain has ESBL (beta lactamase) positive strain, comprises 3 strain Escherichia coli and 2 Pseudomonas aeruginosa strains and 2 strain Klebsiella Pneumoniaes; 2 strain methicillin-resistant staphylococcus aureus (MRSA).
Reference culture has staphylococcus aureus (ATCC6538), Escherichia coli (8099), pseudomonas aeruginosa (ATCC15442), Mycobacterium chelonei abscess subspecies (ATCC19977) and Candida albicans (ATCC10231), be all purchased from national CDC.
1.2 bacterium liquid and bacterial carrier preparation
Get 24h fresh cultured thing, with l0L peptone phosphate buffer (PBS), be mixed with test organisms suspension.Get bacteria suspension 0.02ml drip dye at aseptic degreasing white plain sheet (10mm X 10mm) upper, standby after dry.
1.3 neutralizers are selected test
Take Escherichia coli and Candida albicans as test organisms, and 6 groups of experimental scheme, are undertaken by carrier quantitative germicidal test program.Result judgement is selected satisfactory neutralizer according to version < < disinfection technology standard > > regulation in 2002.
1.4 carrier quantitative germicidal test
Consumption by 1 bacterium sheet 5.0ml, adds thimerosal (positive control is PBS) in aseptic ware, puts preheating 5min in 18～22 ℃ of water-baths, then adds bacterium sheet.Effect certain hour, moves into bacterium sheet 5.0ml neutralizer to be housed in vitro.Neutralization 10 min, beat test tube to wash on lower bacterium sheet bacterium and to mix.Get eluent 0.5ml inoculation plate, pour into ordinary nutrient agar (Candida albicans is sabouraud's agar) and mix, in 37 ℃, cultivate 48h, counting clump count, calculating killing rate.While observing organic substance influence, in bacteria suspension, add variable concentrations calf serum, standby with this collecting cells bacterium sheet.Test is undertaken by carrier quantitative germicidal test program.
2 result of the tests
2.1 neutralizers are selected test
With the PBS containing 5g/L sodium thiosulfate, 30g/L lecithin and 30g/L Tween 80, neutralize respectively hundred million people and pacify disinfectant liquid for skin and the rotten disinfectant of hundred million people's security protections; With containing the PBS of 5g/L sodium thiosulfate, 3g/L lecithin and 30g/L Tween 80 to point and health disinfectant liquid for skin is tested; With containing in the PBS of 1g/L histidine, 3g/L lecithin and 30g/L Tween 80 and the clean hand sanitizing fluid of poem; Above-mentioned four kinds of neutralizers and concentration all reach the requirement of defined.
2.2 bactericidal assay results (in Table 1)
Note: positive controls bacterium number is 1600000～3800000 cfu/ sheets.Result is 5 test mean values.
Note: positive controls bacterium number is 1600000～3800000 cfu/ sheets.Result is 5 test mean values.
Table 1 result shows, hundred million people pacify disinfectant liquid for skin stoste and the rotten disinfectant of hundred million people's security protections to 9 strain clinical isolates and 5 strain standard bacterium effect 1min, and average kill ratio reaches more than 99.9%; Point and health disinfectant liquid for skin stoste to all reference cultures and 8 strain clinical isolates strain effect 1min, average kill ratio >99.9%, the clean hand sanitizing fluid stoste of poem is to 4 strain standard bacterium and 8 strain clinical isolates effect 1min, average kill ratio reaches more than 99.9%, and Mycobacterium chelonei abscess subspecies effect 10min killing rate is only reached to 89.98%.
2.3 organic substance influence tests
Result experiment results proved, containing volume fraction, to be 25% and 50% calf serum kill Escherichia coli, Candida albicans, Mycobacterium chelonei abscess subspecies to four kinds of disinfectant liquid for skin stostes certain influence (table 2)
Note: positive controls bacterium number is that 2300000-3600000 cfu/ sheet results are 5 test mean values.
3 results
Result of the test shows, to clinical isolates, strain has good killing effect to four kinds of disinfectant liquid for skin, with hundred million people, pacifies disinfectant liquid for skin and the rotten disinfectant fungicidal spectrum of hundred million people's security protections is wider, and reference culture and clinical isolates strain killing effect are all relatively good.
Claims (6)
1. a disinfectant liquid for skin for antibiotic and sterilizing, is characterized in that: it is to be made by the raw material in following weight range:
Bo-chrysanthemum 550-650 kilogram of honeysuckle 65-75 kilogram of climbing groundsel 65-75 kilogram
Dandelion 65-75 kilogram of fruit of summer cypress 65-75 kilogram of peppermint 65-75 kilogram
Ethanol 50-100 kilogram of chlorohexidene 35-45 kilogram.
2. disinfectant liquid for skin according to claim 1, wherein the consumption of each raw material is:
70 kilograms of 70 kilograms of climbing groundsels of 600 kilograms of honeysuckles of bo-chrysanthemum
70 kilograms of 70 kilograms of peppermints of 70 kilograms of fruit of summer cypress of dandelion
40 kilograms of 60 kilograms of chlorohexidenes of ethanol.
3. according to the preparation method of disinfectant liquid for skin described in claim 1 or 2, it comprises the following steps:
(1) get the raw materials ready
(1) bo-chrysanthemum: choose clean leaf stalk, anthocaulus and spare impurity;
(2) honeysuckle: sieve removes silt, chooses clean impurity;
(3) climbing groundsel: get crude drug, remove impurity, clean, slightly dry in the air, segment, dry;
(4) dandelion: choose decontamination, clean earth, segment, dries;
(5) fruit of summer cypress: get crude drug, remove impurity, sieve removes chip;
(6) peppermint: choose clean impurity, remove undesirable root, first leaf is trembled and separately put down, then stem is sprayed to clear water, run through rear segment, dry, then with leaf and even;
(7) chlorohexidene: extract according to quantity;
(8) ethanol: extract according to quantity;
(2) preparation
(1) bo-chrysanthemum of described weight, honeysuckle, climbing groundsel, dandelion, the fruit of summer cypress, peppermint are put into the container of boiling medicine, decoct twice, add for the first time 8 times of amounts of water, soak 30 minutes, 80-120 ℃ decocts 1.5 hours, uses inorganic ceramic membrane separation for removing impurities, obtains decoction liquor for the first time;
(2) dregs of a decoction of filtration are added to 4 times of amounts of water, 80-120 ℃ decocts 1 hour, uses hollow fiber ultrafiltration membrane separating and filtering, obtains decoction liquor for the second time;
(3) merge twice decoction liquor, cooling at static 20-30 minute below 40 ℃, 200 eye mesh screens filter twice, get supernatant, then with nanofiltration and reverse osmosis membrane dehydration, concentrate extraction active component, obtain Chinese medical extract;
(4) chlorohexidene of above-mentioned weight is added in alcohol solvent and heats 60 ℃, stir, obtain liquor hibitane;
(5) liquor hibitane after dissolving is added to mixing and stirring in traditional Chinese medicine extract, obtain disinfectant liquid for skin.
4. a corrosion-proof and sterilization agent for antibiotic and sterilizing, is characterized in that: it is to be made by the raw material in following weight range:
Climbing groundsel 20-30kg honeysuckle 20-30kg chrysanthemum 20-30kg
Peppermint 20-30kg ethanol 15-25kg is to chloroxylenol 0.4-0.6kg.
5. corrosion-proof and sterilization agent according to claim 1, wherein the consumption of each raw material is:
Climbing groundsel 25kg honeysuckle 25kg chrysanthemum 25kg
Peppermint 25kg ethanol 20kg is to chloroxylenol 0.5kg.
6. according to the preparation method of corrosion-proof and sterilization agent described in claim 1 or 2, it comprises the following steps:
(1) get the raw materials ready
(1) climbing groundsel: get crude drug, remove impurity, clean, slightly dry in the air, segment, dry;
(2) honeysuckle: sieve removes silt, chooses clean impurity;
(3) chrysanthemum: choose clean leaf stalk, anthocaulus and spare impurity;
(4) peppermint: choose clean impurity, remove undesirable root, first leaf is trembled and separately put down, then stem is sprayed to clear water, run through rear segment, dry, then with leaf and even;
(5) ethanol: extract according to quantity;
(6) to chloroxylenol: extract according to quantity;
(2) preparation
(1) by the climbing groundsel of described weight, honeysuckle, chrysanthemum, peppermint boiling secondary, add for the first time 8 times of amounts of water, soak after 30 minutes, 80-120 ℃ decocts 1.5 hours, uses inorganic ceramic membrane separation for removing impurities, obtains decoction liquor for the first time;
(2) dregs of a decoction of filtration are added to 6 times of amounts of water, 80-120 ℃ decocts 1 hour, uses hollow fiber ultrafiltration membrane separating and filtering, obtains decoction liquor for the second time;
(3) merge twice decoction liquor, cooling at static 20-30 minute below 40 ℃, 200 mesh sieves filter secondary, get supernatant, then with nanofiltration and reverse osmosis membrane dehydration, concentrate extraction active component, obtain Chinese medical extract;
(4) by described weight chloroxylenol is dissolved in the ethanol of described weight, stir 10 minutes, obtain chloroxylenol liquid;
(5) will add in traditional Chinese medicine extract chloroxylenol liquid, mixing and stirring, obtains corrosion-proof and sterilization agent.
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