CN103918927A - Preparation method of stichopus japonicas bait - Google Patents
Preparation method of stichopus japonicas bait Download PDFInfo
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- CN103918927A CN103918927A CN201410164237.3A CN201410164237A CN103918927A CN 103918927 A CN103918927 A CN 103918927A CN 201410164237 A CN201410164237 A CN 201410164237A CN 103918927 A CN103918927 A CN 103918927A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention relates to a preparation method of a stichopus japonicas bait, aiming at solving the technical problem that the existing trepang feed is high in cost and unstable in effect. The preparation method comprises the following steps: adding water to a mixture of vinasse, kelp residue, mixed microorganism bacterium liquid, kelp powder, sargassum thunbergii powder, gulfweed powder and shell powder and stirring to be uniform, performing solid fermentation for 2-6 days under the condition of the temperature of 20-35 DEG C, grinding, and adding sea mud to obtain the stichopus japonicas bait. The preparation method is used for the field of preparation of the stichopus japonicas bait.
Description
Technical field
The present invention relates to a kind of preparation method of bait, is a kind of preparation method of Stichopus japonicus Selenka bait specifically.
Background technology
Along with sea cucumber is propagated the flourish of industry artificially, the digestive physiology of sea cucumber and the research of nutritional need also start starting, the research of digestive ferment in sea cucumber body is impelled to the exploitation of sea cucumber Articial bait making, promoted to a certain extent the development of breading sea cucumber seedlings and aquaculture industry.But along with further developing of holothruian cultures industry, day by day deficient as the various algae resources of Articial bait making main body, its price straight line rises, and the cost that causes sea cucumber Articial bait making improves greatly.
Sea cucumber enteron aisle contains numerous digestive ferments and microorganism, contributes to digestion and the absorption of mixed feed, and even so, part bait still digestibility is still lower.In conjunction with the present Research of other biological bait, the fermentation bait of sea cucumber arises at the historic moment.Fermentation bait not only can the large molecule of external digestion, hard-degraded substance, can also supplement a large amount of active materials and digestive ferment, thereby make sea cucumber more easy to digest to nutrients, bait is utilized more fully, improve the metaboilic level of sea cucumber entirety, promote the Fast Growth of sea cucumber, this promotes the development of holothruian cultures industry undoubtedly by the source of the sea cucumber bait that greatly increases.
In brewex's grains, main composition is protein and dietary fiber, the constituent of brewex's grains can because the kind of the barley that adopts and the addition of auxiliary material and zymotechnique difference and change, general containing protein 24-28%, fat 8-10%, soluble sugar 1-3%, remaining is fiber, its composition is compared with wheat bran, rice bran, granulated slag, corn flour etc., main component is consistent, and content is difference slightly, is still better than above-mentioned all kinds of material on some composition.The aminoacid ingredient of brewers grain protein matter is consistent with aminoacid ingredient in barley, containing a high proportion of paddy ammonia phthalein amine, proline and a small amount of basic amino acid and a certain amount of cysteine, its Glutamic Acid (Glu) and paddy ammonia phthalein amine (Gln) rich content, account for 22.5% of brewex's grains total amino acid.In brewex's grains, containing a large amount of dietary fibers, be rich in solubility pentosan, is good dietary fiber sources.
Kelp residue is in sodium alginate production process, the suspended solid component that the insoluble component that the sea-tangle after immersion obtains through alkali lye digestion, coarse filtration and glue obtain through dilution, foaming, after floating.Show in the existing part test of China and research, kelp residue can be used as feed addictive, it is as the research of aquatic feeds, present stage has also been obtained certain effect: as the constituent using kelp residue as tilapia feed, and with other plant forage components compatibility, addition accounts for 5%～30%, carries out Tilapia mossambica feeding trial, has obtained gratifying result.This just shows, kelp residue can be applied in the middle of the bait of this class omnivorousness aquatic livestock of picture sea cucumber completely.
Holothurian feed is mainly powdery compound feed and pellet now, and these two types of feeds cruelly spill some drawbacks in actual nursing process.Powdery compound feed after splashing, the water-soluble proportion difference of individual class material, what produce falls the heavy different drop of speed, can cause long muddy water, and muddy water can reduce the dissolved oxygen in water, the sea cucumber under anaerobic condition can take in sail and food ration.The ratio coordinating in theory has not existed after overboard, affects growing of sea cucumber.Although pellet has solved the problems referred to above, bait particle is difficult for greatly sea cucumber and absorbs.After pellet sedimentation, sea cucumber can not directly ingest, and after long-time alveolation, decomposition, will affect water quality condition, is unsuitable for equally sea cucumber and grows.The research of sea cucumber Articial bait making also has many aspects need to explore and check at present, in the design of sea cucumber bait formula and the selection of bait raw material, should strengthen research dynamics from now on, to developing the artificial cooperation fermentation bait of Cheap highly effective, effect stability.
Summary of the invention
The present invention is exactly that existing holothurian feed cost is high, the unsettled technical problem of effect in order to solve, and provides that a kind of cost is low, the preparation method of the good Stichopus japonicus Selenka bait of effect.
For this reason, the present invention is with vinasse, kelp residue, mixed microorganism bacterium liquid, Kelp Powder, sargassum thunbergii powder, sargassum powder, oyster shell whiting, poach, and solid fermentation 2～6 days under the condition of 20 DEG C～35 DEG C adds ooze and obtains stichopus japonicus fermentation bait after grinding; The weight percent proportioning of described fermentation raw material is: vinasse 30-50%, mixed microorganism bacterium liquid 10～20%, Kelp Powder 5～10%, sargassum thunbergii powder 1～5%, sargassum powder 1～5%, oyster shell whiting 1～5%, remaining is kelp residue.
Preferably, the bacterium number in mixed microorganism bacterium liquid should be greater than 300,000,000/milliliter, and is less than 1,000,000,000/milliliter.
Preferably, solid fermentation product water content is below 10%, and the sealing holding time is more than 3 months.
Preferably, solid fermentation product is ground to 100-200 order, adds ooze, obtain Stichopus japonicus Selenka bait.
Compared with prior art, the present invention has the following advantages:
1, production technology is simple, easy operating.This product adopts solid fermentation method, and culture medium and condition of culture used in sweat are simple, and solid fermentation adopts heavy dose of liquid inoculation, plays warm fast, duration length, effective in sweat.
2, raw material sources is wide, cost is low, be easy to popularization.This product is mainly taking brewing industry discarded object and seaweed industry waste material etc. as fermentation raw material, through the fermentation of beneficial microbe colony, make the raw materials for production of low value change the high-quality microbial fermentation bait that contains the biological short long factor such as active biological protein, biological small peptides into, can be widely used in the cultivation of stichopus japonicus, promote the development of holothruian cultures industry.
3, with short production cycle.This product adopts microbial solid fermentation technology, and in solid fermentation process, inoculation beneficial microbe colony, selects to be suitable for the culture medium that microbial reproduction is grown, and under the condition that is applicable to growth of microorganism, ferments.Make that microorganism species growth and breeding is fast, bacterium number is high, fermenting raw materials speed is fast, the miscellaneous bacteria of avoiding infection, fermenting and producing security performance is good, to eco-friendly, has ensured product quality.This is a kind of production method of microbial fermentation bait for sea cucumbers of high-quality microorganism species Fast-propagation growth.
4, good product quality, stable performance.The present invention adopts microorganism mixed bacteria liquid as microorganism zymophyte, flora produces abundant nutriment in metabolic process, mainly contain the material that biological small peptides, amino acid, polysaccharide etc. promote biological growth, to promoting the metabolic function of young children's ginseng stomach, the immunity that improves young children's ginseng body has important function, and can reduce disease, and accelerate sea cucumber growth rate, improve culture water quality of tank system.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further:
The present embodiment mixed microorganism bacterium liquid used is the mixed bacteria liquid that has adapted to briny environment, and this bacterium liquid is purchased from coordinate No. 1, former dew of safe and sound bio tech ltd, Dalian hundred.Bacterium liquid mainly contains lactic acid bacteria, saccharomycete and bacillus and forms, and total bacteria count is 5-8*108cfu/mL, and pH is at 4.0-4.5.
Embodiment 1
With vinasse 1250g (50%), kelp residue 575g (23%), Kelp Powder 250g (10%), sargassum thunbergii powder 25g (1%), sargassum powder 125g (5%), oyster shell whiting 25g (1%) are mixed into raw material by a certain percentage, add the microorganism mixed bacteria liquid of 10% (0.25L), psychrometric ratio 1:1, thickness of feed layer 15cm, 25 DEG C of fermentation temperatures, fermentation 6 days, to obtain fermented product be pitchy, be slightly clamminess, the fermentation bait of fine texture, after dry through pulverizing, cross 100 eye mesh screens, obtain fermentation bait.Mass output rate: 89.04%, protein content: 19.05%.
In 60L glass jar, cultivate young ginseng and the each 200-400 gram of children's ginseng, day feeding volume is 3% of sea cucumber fresh weight, evening feeding, the ratio of ooze and bait is 3:1, day quantity of exchanged water is breeding water body 1/2, cultivation temperature 20-23 degree, culturing time 30 days, the rate of rise of 30 days young ginsengs is 122%, and the rate of rise of children's ginseng is 105%.Sea cucumber state is normal, and in water body, ammonia nitrogen, nitrite concentration are lower than below 0.1mg/L.
Embodiment 2
With vinasse 3000g (30%), kelp residue 3400g (34%), Kelp Powder 500g (5%), sargassum thunbergii powder 500g (5%), sargassum powder 100g (1%), oyster shell whiting 500g (5%) are mixed into raw material by a certain percentage, add the microorganism mixed bacteria liquid of 20% (2L), psychrometric ratio 1:1, thickness of feed layer 15cm, 35 DEG C of fermentation temperatures, fermentation 2 days, to obtain fermented product be pitchy, be slightly clamminess, the fermentation bait of fine texture, after dry through pulverizing, cross 200 eye mesh screens, obtain fermentation bait.Mass output rate: 89.04%, protein content 19.29%.
In the cement pit of 1 cubic metre, cultivate each 1 jin of young ginseng and children's ginseng, 670 of young ginsengs, 65 of children's ginsengs, day feeding volume is that number of times is fed 2 times in 3% day of sea cucumber fresh weight, be material 2% evening, feed 1% morning, the ratio of ooze and bait is 3:1, and day quantity of exchanged water is breeding water body 1/2, cultivation temperature 20-22 degree, culturing time 14 days, the rate of rise of 14 days young ginsengs is 184.4%, the rate of rise of children's ginseng is 133%.
Embodiment 3
With vinasse 8Kg (40%), kelp residue 5.6Kg (28%), Kelp Powder 1.6Kg (8%), sargassum powder 0.4Kg (2%), sargassum thunbergii 0.6Kg (3%), oyster shell whiting 0.8Kg (4%) is mixed into raw material by a certain percentage, add the microorganism mixed bacteria liquid of 15% (3L), psychrometric ratio 1:1.8, pack in the plastic box of 100L, thickness of feed layer 20-25cm, 25 DEG C of fermentation temperatures, ferment 5 days, obtaining fermented product is pitchy, slightly be clamminess, the fermentation bait of fine texture, after dry through pulverizing, cross 160 eye mesh screens, obtain fermentation bait.Mass output rate: 91.04%, protein content 19.29%.
In the glass jar of 10 100L rice, cultivate young ginseng and the each 200g of children's ginseng, day feeding volume is that number of times is fed 2 times in 3% day of sea cucumber fresh weight, be material 2% evening, feed 1% morning, the ratio of ooze and bait is 3:1, and day quantity of exchanged water is breeding water body 1/3, cultivation temperature 19-21 degree, culturing time 14 days, the rate of rise of 14 days average young ginsengs is 167.4%, the rate of rise of average children's ginseng is 123%.
Claims (4)
1. the preparation method of a Stichopus japonicus Selenka bait, it is characterized in that with vinasse, kelp residue, mixed microorganism bacterium liquid, Kelp Powder, sargassum thunbergii powder, sargassum powder, oyster shell whiting, poach, solid fermentation 2～6 days under the condition of 20 DEG C～35 DEG C adds ooze and obtains stichopus japonicus fermentation bait after grinding; The weight percent proportioning of described fermentation raw material is: vinasse 30%～50%, mixed microorganism bacterium liquid 10～20%, Kelp Powder 5～10%, sargassum thunbergii powder 1～5%, sargassum powder 1～5%, oyster shell whiting 1～5%, kelp residue.
2. the preparation method of Stichopus japonicus Selenka bait according to claim 1, is characterized in that the bacterium number in described mixed microorganism bacterium liquid should be greater than 300,000,000/milliliter, and is less than 1,000,000,000/milliliter.
3. the preparation method of Stichopus japonicus Selenka bait according to claim 1 and 2, is characterized in that solid fermentation product water content is below 10%, and the sealing holding time is more than 3 months.
4. the preparation method of Stichopus japonicus Selenka bait according to claim 3, is characterized in that solid fermentation product to be ground to 100-200 order, adds ooze, obtains Stichopus japonicus Selenka bait.
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| CN201410164237.3A CN103918927A (en) | 2014-04-23 | 2014-04-23 | Preparation method of stichopus japonicas bait |
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| CN201410164237.3A CN103918927A (en) | 2014-04-23 | 2014-04-23 | Preparation method of stichopus japonicas bait |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472960A (en) * | 2015-01-06 | 2015-04-01 | 江苏省海洋资源开发研究院(连云港) | Anti-stress bait for polyculture of haliotis diversicolor aquatilis and stichopus japonicus |
| CN105124322A (en) * | 2015-10-13 | 2015-12-09 | 威海光洋生物科技有限公司 | Feed used for sea cucumber and preparation method thereof |
| CN106562131A (en) * | 2016-11-11 | 2017-04-19 | 青岛潘托斯生物技术有限公司 | Kelp residue holothurian feed and aquaculture application thereof |
| CN107259215A (en) * | 2017-06-30 | 2017-10-20 | 烟台市水产研究所 | A kind of stichopus japonicus nursery bait and preparation method thereof, application method |
| CN113115729A (en) * | 2020-01-10 | 2021-07-16 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Method for feeding sea cucumber seedlings by mixing granulated feed and fermented feed |
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| CN102919611A (en) * | 2012-11-16 | 2013-02-13 | 威海金牌生物科技有限公司 | Manufacturing method of fermented bait for larval sea cucumber |
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2014
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472960A (en) * | 2015-01-06 | 2015-04-01 | 江苏省海洋资源开发研究院(连云港) | Anti-stress bait for polyculture of haliotis diversicolor aquatilis and stichopus japonicus |
| CN105124322A (en) * | 2015-10-13 | 2015-12-09 | 威海光洋生物科技有限公司 | Feed used for sea cucumber and preparation method thereof |
| CN106562131A (en) * | 2016-11-11 | 2017-04-19 | 青岛潘托斯生物技术有限公司 | Kelp residue holothurian feed and aquaculture application thereof |
| CN107259215A (en) * | 2017-06-30 | 2017-10-20 | 烟台市水产研究所 | A kind of stichopus japonicus nursery bait and preparation method thereof, application method |
| CN113115729A (en) * | 2020-01-10 | 2021-07-16 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Method for feeding sea cucumber seedlings by mixing granulated feed and fermented feed |
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Application publication date: 20140716 |