CN103884701A - Mercury ion detection method - Google Patents
Mercury ion detection method Download PDFInfo
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- CN103884701A CN103884701A CN201410140977.3A CN201410140977A CN103884701A CN 103884701 A CN103884701 A CN 103884701A CN 201410140977 A CN201410140977 A CN 201410140977A CN 103884701 A CN103884701 A CN 103884701A
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- mercury ion
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Abstract
The invention discloses a mercury ion detection method which is characterized by comprising the following steps: (1) mixing a standard mercury ion solution and a gold nanocluster solution, and establishing a standard curve for detecting the fluorescence signal quenching of nanocluster and the mercury ion quantity; and (2) mixing a sample solution with the gold nanocluster solution, substituting the quenching intensity of the fluorescence signal of the nanocluster into the standard curve, thereby obtaining the content of the mercury ion in the sample. The method has the advantages that the sensitivity is high, the interference of other ions on detection of the mercury ion is slight, and selective detection of the mercury ion can be realized, so that the aim of rapidly detecting the mercury ion concentration is achieved. The method has wide application prospects in mercury ion detection.
Description
Technical field
The present invention relates to harmful ion and detect, specifically a kind of detection method of mercury ion.
Background technology
The detection of mercury ion is significant to environment and human health.
At present, the detection method of mercury mainly contains Atomic absorption/emission spectrum, X diffraction fluorescence spectrum, inductively coupled plasma mass spectrometry and the cold steam atomic fluorescence spectrophotometry of selectivity etc., has the drawback of required instrument costliness.
Therefore, develop efficient, cheap, mercury detection technique becomes important in recent years research topic easily and efficiently.
goal of the invention
The detection method of a kind of mercury ion that object of the present invention provides based on above-mentioned prior art situation just, utilizes the method can realize the fast detecting of ion concentration of mercury.
The object of the invention is to be achieved through the following technical solutions:
A detection method for mercury ion, comprises the steps:
1) standard mercury ion solution and gold nanoclusters solution are mixed, set up the typical curve between fluorescence signal cancellation and the mercury ion amount that detects nano-cluster;
2) unknown concentration mercury ion sample solution and gold nanoclusters solution are mixed, bring the fluorescence signal cancellation intensity that detects nano-cluster into typical curve, obtain the content of mercury ion in sample.
The series concentration scope of described standard mercury ion solution is: 12499.2-24.425 nM(nanomole).
Described gold nanoclusters solution is formulated by the following method: gold nanoclusters is dissolved in PBS, regulates pH value to 3-4.5, obtain gold nanoclusters solution; The concentration of gold nanoclusters in solution is calculated as 0.5-2 mM with gold ion concentration.
In the preparation of gold nanoclusters solution, be to regulate pH value by the aqueous solution of phosphoric acid or sodium dihydrogen phosphate.
The structure of described gold nanoclusters is as follows: described gold nanoclusters is modified by human albumin HSA parcel, and decorative layer is HSA, and the particle diameter of described gold nanoclusters is 3-5 nm; The emission wavelength of gold nanoclusters is 725 nm; The excitation wavelength of gold nanoclusters is 500-550nm.
Described gold nanoclusters is also that quantum dot is prepared as follows:
1) in 0.5mL 10 mM gold chlorides, add 0.5 mL 50 mg/mL human albumin HSA, magnetic stirs,
2) above-mentioned solution is regulated to pH=12 with NaOH;
3) microwave is synthetic, and 300w, the time of 100s, obtains described gold nanoclusters.
While detecting fluorescence, instrument is Fluorescence spectrophotometer.
The invention has the advantages that: highly sensitive, other ion pair mercury ion detecting are disturbed little, can realize the selectivity detection to mercury ion, thereby reach the object of fast detecting ion concentration of mercury.This invention has broad application prospects in the detection of mercury ion.
Brief description of the drawings
Fig. 1 is the phenogram of the present invention's quantum dot used, and wherein: A is quantum dot transmission electron microscope picture, B is fluorescent exciting spectrogram.
Fig. 2 is the interference test that gold nanoclusters detects mercury ion, wherein: A is that each metallic ion adds fluorogram after nano-cluster.B is fluorescence intensity level figure.
Fig. 3 is that different ion concentration of mercury detect the fluorescence emission spectrogram in solution at gold nanoclusters.
Fig. 4 is ion concentration of mercury and gold nanoclusters fluorescence intensity graph of a relation and range of linearity figure, wherein: A is ion concentration of mercury and nano-cluster fluorescence intensity cancellation graph of a relation.B is range of linearity figure.
Embodiment
The present invention is described further below in conjunction with accompanying drawing:
Material, reagent etc. used in following embodiment, if no special instructions, all can obtain from commercial channels.
Intermediate water described in literary composition (conductivity 18.2 M Ω) is ultrapure water.
A detection method for mercury ion, comprises the steps:
1) standard mercury ion solution and gold nanoclusters solution are mixed, set up the typical curve between fluorescence signal cancellation and the mercury ion amount that detects nano-cluster;
2) sample solution and gold nanoclusters solution are mixed, bring the fluorescence signal cancellation intensity that detects nano-cluster into typical curve, obtain the content of mercury ion in sample.
The series concentration of described standard mercury ion solution is respectively: 24.425,48.83,97065,195.3,390.6,781.2,1562.4,3124.8, and 6249.6,12499.2 nM
.
Described gold nanoclusters solution is formulated by the following method: gold nanoclusters is dissolved in PBS, to 3-4.5, obtains gold nanoclusters solution by the aqueous solution adjusting pH value of phosphoric acid or sodium dihydrogen phosphate; The concentration of gold nanoclusters in solution is calculated as 0.5-2 mM with gold ion concentration.
Described gold nanoclusters is also that quantum dot is prepared as follows:
1) in 0.5mL 10 mM gold chlorides, add 0.5 mL 50 mg/mL human albumin HSA, magnetic stirs,
2) above-mentioned solution is regulated to pH=12 with NaOH;
3) microwave is synthetic, and 300w, the time of 100s, obtains described gold nanoclusters.
The gold nanoclusters goods that obtain are light brown clear solution, have good water-soluble.
The structure of described gold nanoclusters is as follows: described gold nanoclusters is modified by human albumin HSA parcel, and decorative layer is HSA, and the particle diameter of described gold nanoclusters is 3-5 nm; The emission wavelength of gold nanoclusters is 725 nm; The excitation wavelength of gold nanoclusters is 500-550nm.
Characterize: transmission electron microscope carries out scanner uni excitation spectrum and emission spectrum to above-mentioned quantum-dot structure, and result as shown in Figure 1.Transmission electron microscope picture shows, the quantum dot of synthesized is spherical in shape, and particle diameter is evenly distributed.
Method: use luminoscope to carry out fluorometric assay, first fixed transmission wavelength is 700 nm, scanning fluorescence excitation spectrum, obtains maximum excitation wavelength 525 nm; Taking 525 nm as excitation wavelength, scan quantum dot fluorescence emission spectrum again.
Result: fluorescence excitation spectrum shows that this quantum dot has wider excitation wavelength range, from 500 nm to 550 nm; Fluorescent emission wavelength is in 725 nm left and right.
Example
1) standard mercury ion solution and gold nanoclusters solution are mixed, set up the typical curve between fluorescence signal cancellation and the mercury ion amount that detects nano-cluster;
2) unknown concentration mercury ion solution and gold nanoclusters solution are mixed, bring the fluorescence signal cancellation intensity that detects nano-cluster into typical curve, obtaining mercury ion fluorescent intensity in sample is 160, by I/I
0be worth 0.727 substitution standard equation, obtaining ion concentration of mercury is 1019.5 nM.
Claims (7)
1. a detection method for mercury ion, is characterized in that: comprise the steps:
1) standard mercury ion solution and gold nanoclusters solution are mixed, set up the typical curve between fluorescence signal cancellation and the mercury ion amount that detects nano-cluster;
2) unknown concentration mercury ion sample solution and gold nanoclusters solution are mixed, bring the fluorescence signal cancellation intensity that detects nano-cluster into typical curve, obtain the content of mercury ion in sample.
2. the detection method of mercury ion according to claim 1, is characterized in that: the series concentration scope of described standard mercury ion solution is: 12499.2-24.425 nM.
3. the detection method of mercury ion according to claim 1, is characterized in that: described gold nanoclusters solution is formulated by the following method: gold nanoclusters is dissolved in PBS, regulates pH value to 4.5, obtain gold nanoclusters solution; The concentration of gold nanoclusters in solution is calculated as 1 mM with gold ion concentration.
4. the detection method of mercury ion according to claim 3, is characterized in that: in the preparation of gold nanoclusters solution, be to regulate pH value by the aqueous solution of phosphoric acid or sodium dihydrogen phosphate.
5. the detection method of mercury ion according to claim 1, is characterized in that: the structure of described gold nanoclusters is as follows: described gold nanoclusters is modified by human albumin HSA parcel, and decorative layer is HSA, and the particle diameter of described gold nanoclusters is 3-5 nm; The emission wavelength of gold nanoclusters is 725 nm; The excitation wavelength of gold nanoclusters is 540nm.
6. the detection method of mercury ion according to claim 5, is characterized in that: described gold nanoclusters is also that quantum dot is prepared as follows:
1) in 0.5mL 10 mM gold chlorides, add 0.5 mL 50 mg/mL human albumin HSA, magnetic stirs,
2) above-mentioned solution is regulated to pH=12 with NaOH;
3) microwave is synthetic, the time of 300W 100s, obtains described gold nanoclusters.
7. the detection method of mercury ion according to claim 1, is characterized in that: while detecting fluorescence, instrument is Fluorescence spectrophotometer.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215760A (en) * | 2014-09-13 | 2014-12-17 | 福建医科大学 | Urease inhibitor determination method based on fluorescence gold nano cluster |
| CN104745194A (en) * | 2015-03-24 | 2015-07-01 | 南昌大学 | Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection |
| CN104801722A (en) * | 2015-03-13 | 2015-07-29 | 武汉理工大学 | Preparation method of human serum albumin gold nanoclusters |
| CN105572087A (en) * | 2016-01-11 | 2016-05-11 | 东北师范大学 | Mercury ion colorimetric detection test paper capable of being cyclically used and application method thereof |
| CN105675519A (en) * | 2016-04-01 | 2016-06-15 | 江南大学 | Mercury ion detection method |
| CN106198474A (en) * | 2016-07-20 | 2016-12-07 | 郑州大学 | A kind of mercury ion test paper and using method thereof |
| CN106908427A (en) * | 2017-03-01 | 2017-06-30 | 哈尔滨师范大学 | Gold nanoclusters and carbon quantum dot composite fluorescence probe and its application |
| CN107024463A (en) * | 2017-06-14 | 2017-08-08 | 江南大学 | Selective Sensitive Detection mercury ion is used for based on gold nanoclusters ratio fluorescent test strip |
| CN108303401A (en) * | 2017-12-18 | 2018-07-20 | 中国烟草总公司郑州烟草研究院 | A kind of fluorescence nano composite material and its application in metal ion detection |
| CN111227799A (en) * | 2020-01-21 | 2020-06-05 | 中国农业大学 | In-vivo detection method for brain tissue methylmercury |
| CN111375783A (en) * | 2020-04-17 | 2020-07-07 | 广东医科大学 | Multifunctional HSA-cadmium nanocluster, preparation method and application thereof |
| CN111879747A (en) * | 2020-08-21 | 2020-11-03 | 中国医学科学院生物医学工程研究所 | Mercury ion and copper ion synchronous detection method |
| CN112170859A (en) * | 2020-10-09 | 2021-01-05 | 深圳技术大学 | Preparation method of gold nanocluster |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101713737A (en) * | 2009-12-29 | 2010-05-26 | 东北师范大学 | Fluorescent detection probe for mercury ions and application method thereof |
| CN102150034A (en) * | 2008-08-05 | 2011-08-10 | 新加坡科技研究局 | Methods, compositions, and articles comprising stabilized gold nanoclusters |
| CN103464780A (en) * | 2013-09-06 | 2013-12-25 | 湖南科技大学 | Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin |
-
2014
- 2014-04-10 CN CN201410140977.3A patent/CN103884701A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150034A (en) * | 2008-08-05 | 2011-08-10 | 新加坡科技研究局 | Methods, compositions, and articles comprising stabilized gold nanoclusters |
| CN101713737A (en) * | 2009-12-29 | 2010-05-26 | 东北师范大学 | Fluorescent detection probe for mercury ions and application method thereof |
| CN103464780A (en) * | 2013-09-06 | 2013-12-25 | 湖南科技大学 | Preparation method of fluorescence gold nano clusters with stable chicken ovalbumin |
Non-Patent Citations (3)
| Title |
|---|
| LEI YAN 等: "Microwave-assisted synthesis of BSA-stabilized and HSA-protected gold nanoclusters with red emission", 《JOURNAL OF MATERIALS CHEMISTRY》, vol. 22, 11 November 2011 (2011-11-11), pages 1000 - 1005 * |
| PO-CHENG CHEN 等: "Synthesis of fluorescent BSA–Au NCs for the detection of Hg2+ ions", 《J NANOPART RES》, vol. 15, 24 December 2012 (2012-12-24), pages 1336 * |
| 杜鹃: "金纳米粒子微波及水热反应合成研究及其在光谱分析中的应用", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》, no. 02, 15 February 2014 (2014-02-15) * |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215760B (en) * | 2014-09-13 | 2016-06-29 | 福建医科大学 | Urease inhibitor assay method based on fluorogold nanocluster |
| CN104215760A (en) * | 2014-09-13 | 2014-12-17 | 福建医科大学 | Urease inhibitor determination method based on fluorescence gold nano cluster |
| CN104801722A (en) * | 2015-03-13 | 2015-07-29 | 武汉理工大学 | Preparation method of human serum albumin gold nanoclusters |
| CN104745194A (en) * | 2015-03-24 | 2015-07-01 | 南昌大学 | Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection |
| CN105572087A (en) * | 2016-01-11 | 2016-05-11 | 东北师范大学 | Mercury ion colorimetric detection test paper capable of being cyclically used and application method thereof |
| CN105675519B (en) * | 2016-04-01 | 2018-02-09 | 江南大学 | A kind of mercury ion detecting method |
| CN105675519A (en) * | 2016-04-01 | 2016-06-15 | 江南大学 | Mercury ion detection method |
| CN106198474A (en) * | 2016-07-20 | 2016-12-07 | 郑州大学 | A kind of mercury ion test paper and using method thereof |
| CN106908427A (en) * | 2017-03-01 | 2017-06-30 | 哈尔滨师范大学 | Gold nanoclusters and carbon quantum dot composite fluorescence probe and its application |
| CN107024463A (en) * | 2017-06-14 | 2017-08-08 | 江南大学 | Selective Sensitive Detection mercury ion is used for based on gold nanoclusters ratio fluorescent test strip |
| CN108303401A (en) * | 2017-12-18 | 2018-07-20 | 中国烟草总公司郑州烟草研究院 | A kind of fluorescence nano composite material and its application in metal ion detection |
| CN111227799A (en) * | 2020-01-21 | 2020-06-05 | 中国农业大学 | In-vivo detection method for brain tissue methylmercury |
| CN111375783A (en) * | 2020-04-17 | 2020-07-07 | 广东医科大学 | Multifunctional HSA-cadmium nanocluster, preparation method and application thereof |
| CN111375783B (en) * | 2020-04-17 | 2022-10-11 | 广东医科大学 | Multifunctional HSA-cadmium nanocluster, preparation method and application thereof |
| CN111879747A (en) * | 2020-08-21 | 2020-11-03 | 中国医学科学院生物医学工程研究所 | Mercury ion and copper ion synchronous detection method |
| CN111879747B (en) * | 2020-08-21 | 2023-08-22 | 中国医学科学院生物医学工程研究所 | Synchronous detection method for mercury ions and copper ions |
| CN112170859A (en) * | 2020-10-09 | 2021-01-05 | 深圳技术大学 | Preparation method of gold nanocluster |
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Application publication date: 20140625 |