CN103865839B - A kind of probiotics applied to aquaculture - Google Patents
A kind of probiotics applied to aquaculture Download PDFInfo
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- CN103865839B CN103865839B CN201410014890.1A CN201410014890A CN103865839B CN 103865839 B CN103865839 B CN 103865839B CN 201410014890 A CN201410014890 A CN 201410014890A CN 103865839 B CN103865839 B CN 103865839B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The present invention relates to a kind of preparation applied to aquaculture, specifically made of the flower domain bacillus preparation in aquaculture prevention disease, improve food conversion ratio or purifying aquaculture water quality in application.
Description
Technical field
The present invention relates to a kind of preparation applied to aquaculture, and preparation is in water specifically made of flower domain bacillus
Production cultivation in prevention disease, improve food conversion ratio or purifying aquaculture water quality in application.
Background technology
The end of the year 2006 beat alarm bell in the residual event of turbot medicine of whole nation outburst to us.European Union oneself in 2006 comprehensively
Forbid application of the antimicrobial as feed addictive in cultivation.To ensure the developed country such as food security, the U.S. to meat, sea
The antimicrobials such as product residual is detected.With China's accession to WTO, famous-brand and high-quality goods outlet is generally had an optimistic view of.But due to state
The phenomenon of interior cultivation not hazardous drugs such as scientifical use antibiotic is serious, greatly limit the outlet of product.Exploitation antimicrobial replaces
For products application required for aquaculture is existing market.
The content of the invention
It is an object of the invention to provide one kind in aquaculture prevention disease, improve food conversion ratio or purifying aquaculture water
The preparation of matter, said preparation is by spending domain bacillus to be made.
What the preparation of the preparation of the present invention was implemented preferably through following step, but this preparation technology is not limited to, it is known
The preparation technology that can be realized can be with：The sample of the domain bacillus containing flower is taken, then sample is placed in sterilizing bottle, 4 DEG C
Preserve, therefrom take during research a certain amount of sample to add in the dilution of 18mL sterilizings, fully mix, in aseptic operating platform, enter
Row 10-1、10-2、10-3、10-4, 10-5, 10-6, 10-7Gradient dilution, take 10-5, 10-6, 10-7Three dilution gradients, are respectively coated on
On flower domain bacillus selectivity single bacterium colony separation solid medium, it is placed in incubator, is cultivated 48 hours at 30 DEG C, selection length
The good single bacterium colony of gesture is inoculated in colored domain bacillus liquid amplification culture medium respectively, amplification cultivation 48 hours at 30 DEG C.Will
After gained medium centrifugal (12000rpm) isolates thalline, by thalline vacuum freezedrying, the drying bacterium of each strain is modulated
Powder, then basis《The outstanding Bacteria Identification handbook of uncle》, Related Bacteria identification document or 16S rRNA sequence comparative analysis carry out spending domain
Bacillus is identified and toxicity test, and avirulent colored domain bacillus is dried into bacterium powder desired proportions addition auxiliary material system
Into the various formulations such as granule, tablet, capsule, powder, pulvis or liquid preparation, or be made medicine, veterinary drug, feed addictive or
Water quality cleansing agent, it can also add the oligosaccharide such as other viable bacterias or FOS and play synergy.
Flower domain bacillus selectivity single bacterium colony separation solid medium is preferably but not limited to：Purified water 1L, peptone
10.0g, beef extract 3.0g, NaCl5.0g, agar 25.0g, pH7.2 is adjusted, 121 DEG C, is sterilized 15 minutes.
Flower domain bacillus liquid amplification culture medium is preferably but not limited to：Purified water 1L, peptone 5.0g, dusty yeast
1.0g, ironic citrate 0.1g, sodium chloride 19.45g, magnesium chloride 5.98g, sodium sulphate 3.24g, calcium chloride 1.8g, potassium chloride
0.55g, sodium carbonate 0.16g, KBr 0.08g, strontium chloride 0.034g, boric acid 0.022g, sodium metasilicate 0.004g, sodium fluoride
0.0024g, sodium nitrate 0.0016g, the 0.008g of phosphoric acid hydrogen two, pH7.4 is adjusted, 121 DEG C, is sterilized 15 minutes.
To be further elaborated with the present invention, inventor passes through flower domain bacillus selectivity single bacterium colony using the above method
Separation solid medium separation identifies avirulent colored domain bacillus, and colored domain bacillus of the present invention is not limited to
In flower domain bacillus described to illustrate the invention, as long as avirulent colored domain bacillus is of the present invention, exist
In protection scope of the present invention.
Colored domain bacillus of the present invention is preferably but not limited to spend domain bacillus DHH28 deposit number CGMCC8561,
Flower domain bacillus DHH06 deposit numbers CGMCC8566.
The present invention implements the bacteriological quality of the flower domain bacillus preferably used in explanation, and the present invention does not limit to this hair
The bright bacteriological quality, simply to illustrate the invention preferred：
1st, to illustrate the invention, the present invention is respectively to spend domain bacillus using the flower domain bacillus of above method separation
DHH28 deposit number CGMCC8561, flower domain bacillus DHH06 deposit numbers CGMCC8566.
2nd, thalline and colonial morphology
Micro- sem observation：Long 2.0-2.5 microns, wide 0.7-0.9 microns, elongated rod shape, Gram-positive, gemma is in ellipse
It is raw in circle.
Colonial morphology is in subcircular, neat in edge and sticky moistening, White-opalescent.
3rd, Physiology and biochemistry is identified
Hydrogen sulfide：One；Indoles：One；Gelatin liquefaction：+；Nitrate reduction：One.
4th, glycolysis experimental identification
Glucose：+.
Colored domain bacillus of the present invention refers to living organism individual.
The present invention be the above-mentioned colored domain bacillus using effective dose as main active ingredient, according to certain preparation work
Skill, add conventional excipient, flavor enhancement, disintegrant, preservative, lubricant, wetting agent, binder, solvent, thickener, increasing
The auxiliary materials such as solvent, any formulation being adapted for use with is made, such as tablet, capsule, granule, powder, liquid preparation, powder
The formulations such as agent.
Active bacteria formulation is made as main active ingredient in above-mentioned colored domain bacillus of the present invention using effective dose,
Can be in the probiotics such as above-mentioned colored domain bacillus and Bifidobacterium, streptococcus, bacillus subtilis one or more or
Active bacteria formulation is made in the combination of other active ingredients, to play synergy, improves effect.
The signified effective dose of the present invention refers to be used as master alone or in combination according to described above using above-mentioned colored domain bacillus
The total viable count for wanting solid live bacteria preparation made of medicament active composition to include cannot be below 1 × 106CFU/g, typically 1 ×
109More than CFU/g, it can reach 1 × 1011CFU/g or 1 × 1011More than CFU/g.
The signified effective dose of the present invention refers to be used as master alone or in combination according to described above using above-mentioned colored domain bacillus
The total viable count for wanting liquid active bacteria formulation made of medicament active composition to include cannot be below 1 × 106CFU/mL, typically 1 ×
109More than CFU/mL, it can reach 1 × 1011CFU/mL or 1 × 1011More than CFU/mL.
The first public colored domain bacillus of the present invention in aquaculture prevention disease, improve food conversion ratio or purify foster
The application in water quality is grown, therefore application of the preparation containing above-mentioned colored domain bacillus in above-mentioned belongs to the protection model of the present invention
Enclose.
Preparation is made in sea cucumber, shrimp, Bao as main active ingredient because present invention firstly discloses flower domain bacillus
Application in the cultivation such as fish or fish, therefore the preparation containing above-mentioned colored domain bacillus is in sea cucumber, shrimp, abalone or fish etc. cultivate
Using belonging to protection scope of the present invention.
Colored domain bacillus of the present invention is respectively provided with aquaculture when any formulation is made and prevents disease
Disease, improve food conversion ratio or the effect of purifying aquaculture water quality.Any formulation, if containing above-mentioned colored domain gemma bar in its component
Bacterium composition is prepared into preparation, as long as indicating or prompting in the mark such as its packaging or specification or on other any propaganda materials
There is the prevention disease in aquaculture, improve food conversion ratio or purifying aquaculture water quality, then fall into the guarantor of the present invention
Within the scope of shield.
Veterinary drug, feed addictive or cultivation water cleanser can be made in colored domain bacillus of the present invention.Will be upper
Veterinary drug, feed addictive or cultivation water cleanser made of colored domain bacillus is stated to be applied to prevent disease in aquaculture
Disease, improve food conversion ratio or purifying aquaculture water quality, then fall under the scope of the present invention.
Embodiment
Preparation example explanation：The above-mentioned preparation to flower domain bacillus preparation illustrates, and passes through flower domain gemma here
Bacillus DHH28 deposit number CGMCC8561, flower domain bacillus DHH06 deposit number CGMCC8566 granules exemplified by had
Body illustrates that preparation method those skilled in the art of other flower domain Bacillus preparations are easy to slap by the present embodiment
Hold, preparation method those skilled in the art of other formulations are easy to grasp by this implementation, no longer describe explanation one by one herein.
Preparation method is not limited to described in the embodiment of the present invention, and the known method that can reach preparation purpose can be with embodiment
Preparation explanation be the description of the invention, be not limiting the scope of the invention.
Prepare the preparation of embodiment flower domain bacillus living powder
The preparation of 1 bacterium powder and the identification of strain
The sample (sea cucumber intestinal contents, seawater) containing flower domain bacillus is taken, sample is then placed in sterilizing bottle
It is interior, therefrom take sample segment to add in the dilution of 18mL sterilizings rapidly, fully mix, in aseptic operating platform, carry out 10-1、
10-2、10-3、10-4, 10-5, 10-6, 10-7Gradient dilution, take 10-5, 10-6, 10-7Three dilution gradients, 80 DEG C of water-baths 13 minutes,
It is coated on colored domain bacillus selectivity single bacterium colony separation solid medium, is placed in anaerobic jar, 48 are cultivated at 30 DEG C of anaerobism
Hour, the single bacterium colony for selecting to grow fine is inoculated into colored domain bacillus liquid amplification culture medium, expanded at 30 DEG C of anaerobism respectively
Increase culture 48 hours.After gained medium centrifugal (12000rpm) is isolated into thalline, thalline vacuum freezedrying modulates
Bacterium powder is dried, viable count is 1 × 109More than CFU/g, then basis《The outstanding Bacteria Identification handbook of uncle》, Related Bacteria identification text
Offer and carried out with after isolated strains progress 16S rRNA gene sequencing with the gene order in BLAST and GenBank and RDP databases
Similarity analysis, it is identified as spending domain bacillus DHH28 deposit number CGMCC8561, flower domain bacillus DHH06 preservations are compiled
Number CGMCC8566.
2 toxicity tests
2.1 animals and packet take 30 SPF rank mouse, 6~8 week old, 15~19g of body weight, are randomized into colored domain gemma
Bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups and non-administered group, every group 10.
Above-mentioned different flower domain bacillus bacterium powder is modulated to containing bacterium number be 1 by 2.2 preparation bacterium solutions with purified water respectively
×109CFU/mL bacterium solution.
Each colored domain bacillus group of 2.3 methods and non-administered group give identical basal feed, and rearing conditions are homogeneous
Causing, each colored domain bacillus group gavages colored domain bacillus bacterium solution 0.5mL daily, and non-administered group gavages purified water 0.5mL daily,
Feeding 6 months, observe body weight and toxic reaction.
2.4 result
Each group mouse does not occur abnormal conditions, and chatter, spasm, ataxia, posture exception do not occur, and no eyeball is dashed forward
Go out, urination is normal, and skin, breathing are normal, no death condition, and the increased weight of each colored domain bacillus group be significantly higher than not to
Medicine group (P<0.05), show to spend domain Bacillus to have enhancing development effect, explanation works well, and has no that poisoning is anti-
Should.
3 are prepared into the formulations such as granule
It is non-toxic through experimental check after above-mentioned steps and method separation identification, so that it may by flower domain Bacillus
Bacterium powder is made, then addition relevant auxiliary materials are made various formulations as required, preferably according to the viable count of flower domain bacillus bacterium powder,
Adhesive, zeolite powder etc. are added in proportion granule is made, viable count is not less than 1 × 109CFU/g, then pack.
Application effect embodiment explanation：The present invention spends domain bud to spend domain bacillus DHH28 deposit number CGMCC8561
Spore bacillus DHH06 deposit numbers CGMCC8566 is that representative illustrates to spend application effect of the domain bacillus in aquaculture.
Application effect embodiment 1：Application of the flower domain bacillus in holothruian cultures
1 materials and methods
1.1 material
1.1.1 test products
Flower domain bacillus living pulvis, viable count is 1 × 109More than CFU/g.
1.1.2 sea cucumber is tested
Sea cucumber is divided into 3 groups, every group 50.
1.2 method
1.2.1 test period is 30 days.
1.2.2 the experiment takes sea cucumber conventional feed to feed, and test group adds colored domain bacillus living granule daily,
It is 1 × 10 to ensure water body concentration7More than CFU/L, control group are not added with test products.
2 result of the tests
The influence of 2.1 pairs of digestive ferments
2.1.1 to the influence of protease
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of protease is improved 31.10%, 31.31% respectively, difference has statistical significance (P<0.05), but between test group
No significant difference (P>0.05).
2.1.2 to the influence of amylase
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of amylase is improved 25.02%, 25.11% respectively, difference has statistical significance (P<0.05), but between test group
No significant difference (P>0.05).
The immune influence of 2.2 pairs of enhancings
2.2.1 to the influence of superoxide dismutase
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of superoxide dismutase is improved 11.16 times, 11.61 times respectively, difference has statistical significance, but between test group
No significant difference (P>0.05).
2.2.2 to the influence of lysozyme
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of lysozyme is improved 19.03 times, 19.10 times respectively, difference has statistical significance (P<0.05), but between test group
No significant difference (P>0.05).
The influence of 2.3 pairs of daily gains
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
Daily gain is improved 31%, 32% respectively, difference has statistical significance (P<0.05) no statistical difference, but between test group
Meaning (P>0.05).
2.4 the death rate
As a result show that it is 0 to spend domain bacillus CGMCC8561 groups, the flower domain bacillus CGMCC8566 group death rates, it is low
In 3.15% (P of control group<0.05) no significant difference (P, but between test group>0.05).
The influence of 2.5 pairs of cultivation waters
2.5.1 to pH influence
The anaplasia of flower domain bacillus CGMCC8561 groups, the pH value of flower domain bacillus CGMCC8566 groups 6.9~7.6
Change, control group pH value changes between 6.2~7.8, and test group is small compared to control group amplitude of fluctuation, the statistically significant (P of difference
<0.05) no significant difference (P, but between test group>0.05).2.5.2 to the influence of nitrite, ammoniacal nitrogen
Each test group nitrite, ammoniacal nitrogen are less than the (P of control group 38%<0.05) difference is without statistics, but between test group
Learn meaning (P>0.05).
3 conclusions
Flower domain bacillus can improve sea cucumber daily gain, digestive enzyme activity, superoxide dismutase activity, bacteriolyze enzyme activity
Property, and reduce the death rate and improve water quality, and difference is not statistically significant between test group, illustrates that flower domain bacillus has
Promote the growth of sea cucumber, improve sea cucumber immunity, prevent and treat sea cucumber disease, improve food conversion ratio or the effect of purifying aquaculture water quality
Application effect embodiment 2：Application of the flower domain bacillus in shrimp aquaculture
1 materials and methods
1.1 material
1.1.1 test products
Flower domain bacillus living pulvis, viable count is 1 × 109More than CFU/g.
1.1.2 shrimp is tested
Shrimp is divided into 3 groups, every group of 90 tails, every group sets 3 repetitions.
1.2 method
1.2.1 test period is 30 days.
1.2.2 the experiment takes shrimp conventional feed to feed, and test group adds colored domain bacillus living granule daily, protects
It is 1 × 10 to demonstrate,prove water body concentration7More than CFU/L, control group are not added with test products.
2 result of the tests
2.1 pairs of influences for promoting growth
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
Body weight is improved 18.11%, 17.89% respectively, difference has statistical significance (P<0.05), but between test group difference without
Statistical significance (P>0.05).
The immune influence of 2.2 pairs of enhancings
2.2.1 to the influence of superoxide dismutase
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of superoxide dismutase is improved 10.65%, 10.78% respectively, difference has statistical significance, but between test group
No significant difference (P>0.05).
2.2.2 to the influence of lysozyme
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of lysozyme is improved 9.52%, 9.48% respectively, difference has statistical significance (P<0.05) it is, but poor between test group
Different not statistically significant (P>0.05).
2.3 the death rate
As a result show to spend the domain bacillus CGMCC8561 groups, the flower domain bacillus CGMCC8566 group death rates are respectively
2.12%th, 2.31%, less than 9.56% (P of control group<0.05) no significant difference (P, but between test group>
0.05)。
The influence of 2.4 pairs of feed-weight ratios
As a result show to spend the domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 group feed-weight ratios are respectively
1.32nd, 1.33, less than 1.98 (P of control group<0.05) no significant difference (P, but between test group>0.05).
The influence of 2.5 pairs of cultivation waters
2.5.1 to pH influence
The pH value of test group changes between 6.6~7.4, and control group pH value changes between 6.1~7.9, test group phase
It is smaller than control group amplitude of fluctuation, the statistically significant (P of difference<0.05) no significant difference (P, but between test group>
0.05)。
2.5.2 to the influence of nitrite, ammoniacal nitrogen
Each test group nitrite, ammoniacal nitrogen are less than the (P of control group 36%<0.05) difference is without statistics, but between test group
Learn meaning (P>0.05).
3 conclusions
Flower domain bacillus can improve shrimp body weight, superoxide dismutase activity, lysozyme activity, and reduce death
Rate, feed-weight ratio and improvement water quality, and difference is not statistically significant between test group, is illustrated that flower domain bacillus has and is promoted shrimp
Growth, improve shrimp immunity, prevent and treat shrimp disease, improve food conversion ratio or the effect of purifying aquaculture water quality.
Application effect embodiment 3：Application of the flower domain bacillus in abalone culture
1 materials and methods
1.1 material
1.1.1 test products
Flower domain bacillus living pulvis, viable count is 1 × 109More than CFU/g.
1.1.2 abalone is tested
Abalone is divided into 3 groups, every group 90, every group sets 3 repetitions.
1.2 method
1.2.1 test period is 30 days.
1.2.2 the experiment takes abalone conventional feed to feed, and test group adds colored domain bacillus living granule daily,
It is 1 × 10 to ensure water body concentration7More than CFU/L, control group are not added with test products.
2 result of the tests
2.1 pairs of influences for promoting growth
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
Body weight is improved 13.32%, 13.50% respectively, difference has statistical significance (P<0.05), but between test group difference without
Statistical significance (P>0.05).
The immune influence of 2.2 pairs of enhancings
2.2.1 to the influence of superoxide dismutase
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of superoxide dismutase is improved 15.58%, 15.69% respectively, difference has statistical significance, but between test group
No significant difference (P>0.05).
2.2.2 to the influence of lysozyme
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of lysozyme is improved 12.23%, 12.15% respectively, difference has statistical significance (P<0.05), but between test group
No significant difference (P>0.05).
2.3 the death rate
As a result show to spend the domain bacillus CGMCC8561 groups, the flower domain bacillus CGMCC8566 group death rates are respectively
2.32%th, 2.10%, less than 9.29% (P of control group<0.05) no significant difference (P, but between test group>
0.05)。
The influence of 2.4 pairs of feeds and weight ratio
As a result show to spend the domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 group feed-weight ratios are respectively
1.25th, 1.33, less than 1.88 (P of control group<0.05) no significant difference (P, but between test group>0.05).
The influence of 2.5 pairs of cultivation waters
2.5.1 to pH influence
The pH value of test group changes between 6.5~7.1, and control group pH value changes between 6.1~7.8, test group phase
It is smaller than control group amplitude of fluctuation, the statistically significant (P of difference<0.05) no significant difference (P, but between test group>
0.05)。
2.5.2 to the influence of nitrite, ammoniacal nitrogen
Each test group nitrite, ammoniacal nitrogen are less than the (P of control group 32%<0.05) difference is without statistics, but between test group
Learn meaning (P>0.05).
3 conclusions
Flower domain bacillus can improve abalone body weight, superoxide dismutase activity, lysozyme activity, and reduce dead
Die rate, feed-weight ratio and improve water quality, and difference is not statistically significant between test group, is illustrated that flower domain bacillus has and is promoted
The growth of abalone, abalone immunity is improved, prevent and treat abalone disease, improve food conversion ratio or the effect of purifying aquaculture water quality.
Application effect embodiment 4：Application of the flower domain bacillus in perch cultivates
1 materials and methods
1.1 material
1.1.1 test products
Flower domain bacillus living pulvis, viable count is 1 × 109More than CFU/g.
1.1.2 abalone is tested
Perch is divided into 3 groups, every group 90, every group sets 3 repetitions.
1.2 method
1.2.1 test period is 30 days.
1.2.2 the experiment takes perch conventional feed to feed, and test group adds colored domain bacillus living granule daily,
It is 1 × 10 to ensure water body concentration7More than CFU/L, control group are not added with test products.
2 result of the tests
2.1 pairs of influences for promoting growth
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
Body weight is improved 30.58%, 30.69% respectively, difference has statistical significance (P<0.05), but between test group difference without
Statistical significance (P>0.05).
The immune influence of 2.2 pairs of enhancings
2.2.1 to the influence of superoxide dismutase
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of superoxide dismutase is improved 13.51%, 13.67% respectively, difference has statistical significance, but between test group
No significant difference (P>0.05).
2.2.2 to the influence of lysozyme
As a result show to spend domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 groups compared with control group,
The activity of lysozyme is improved 50.12%, 50.32% respectively, difference has statistical significance (P<0.05), but between test group
No significant difference (P>0.05).
2.3 the death rate
As a result show to spend the domain bacillus CGMCC8561 groups, the flower domain bacillus CGMCC8566 group death rates are respectively
3.12%th, 3.10%, less than 10.12% (P of control group<0.05) no significant difference (P, but between test group>
0.05)。
The influence of 2.4 pairs of feeds and weight ratio
As a result show to spend the domain bacillus CGMCC8561 groups, flower domain bacillus CGMCC8566 group feed-weight ratios are respectively
0.96th, 0.97, less than 1.56 (P of control group<0.05) no significant difference (P, but between test group>0.05).
The influence of 2.5 pairs of cultivation waters
2.5.1 to pH influence
The pH value of test group changes between 6.6~7.5, and control group pH value changes between 6.2~7.8, test group phase
It is smaller than control group amplitude of fluctuation, the statistically significant (P of difference<0.05) no significant difference (P, but between test group>
0.05)。
2.5.2 to the influence of nitrite, ammoniacal nitrogen
Each test group nitrite, ammoniacal nitrogen are less than the (P of control group 35%<0.05) difference is without statistics, but between test group
Learn meaning (P>0.05).
3 conclusions
Flower domain bacillus can improve perch body weight, superoxide dismutase activity, lysozyme activity, and reduce dead
Die rate, feed-weight ratio and improve water quality, and difference is not statistically significant between test group, is illustrated that flower domain bacillus has and is promoted
The growth of perch, perch immunity is improved, prevent and treat perch disease, improve food conversion ratio or the effect of purifying aquaculture water quality.
Application effect embodiment 5：Application of the flower domain bacillus in purifying sea water
Seawater is taken, is divided into three parts, wherein two parts of seawater are separately added into colored domain bacillus CGMCC8561, flower domain gemma bar
Bacterium CGMCC8566, it is 1 × 10 to ensure daily water body concentration7More than CFU/L, another part seawater are control group, are not added with any viable bacteria,
Place 7 days at room temperature, detect seawater, each colored domain bacillus group nitrite, ammoniacal nitrogen are less than the (P of control group 25%<
0.05) no significant difference (P, but between each colored domain bacillus group>0.05).Good breeding environment is provided, in advance
The generation of anti-disease.
Microorganism fungus kind of the present invention used in implementation process is on December 06th, 2013 in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism
Research institute's postcode 100101) preservation, totally 2 following microorganism fungus kinds, but colored domain bacillus of the present invention is not limited to
In this 2 microorganism fungus kind.
(1) Classification And Nomenclature：Flower domain bacillus vallismortis, preserves numbering 8561.
(2) Classification And Nomenclature：Flower domain bacillus vallismortis, preserves numbering 8566.
For above-mentioned 2 microorganism fungus kinds through the microorganism Spot detection, testing result is survival.
Claims (9)
1. spend application of the domain bacillus in aquaculture preparations are prepared, it is characterised in that the aquaculture preparations pass through increasing
Strong immune protection disease, the flower domain bacillus DHH28 for being CGMCC No.8561 by deposit number and/or deposit number are
CGMCC No.8566 flower domain bacillus DHHO6 is made, wherein the aquaculture is sea cucumber, shrimp, abalone or fish culture.
2. applied as described in claim 1, it is characterised in that the aquaculture preparations include veterinary drug, feed addictive.
3. applied as described in claim 1, it is characterised in that flower domain bacillus refers to bion living.
4. applied as described in claim 1, it is characterised in that the aquaculture preparations are solid pharmaceutical preparation, its total viable bacteria included
Number is not less than 1 × 106CFU/g。
5. applied as described in claim 1, it is characterised in that the aquaculture preparations are solid pharmaceutical preparation, its total viable bacteria included
Number preferably 1 × 109CFU/g and more than.
6. applied as described in claim 1, it is characterised in that the aquaculture preparations are solid pharmaceutical preparation, its total viable bacteria included
Number preferably 1 × 1011CFU/g and more than.
7. applied as described in claim 1, it is characterised in that the aquaculture preparations are liquid preparation, its total viable bacteria included
Number is not less than 1 × 106CFU/mL。
8. applied as described in claim 1, it is characterised in that the aquaculture preparations are liquid preparation, its total viable bacteria included
Number preferably 1 × 109CFU/mL and more than.
9. applied as described in claim 1, it is characterised in that the aquaculture preparations are liquid preparation, its total viable bacteria included
Number preferably 1 × 1011CFU/mL and more than.
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