CN103865546B - Method for restoring heavy metal in sewage irrigation soil through NTA and 3-indoleacelic acid-enhanced festuca arundinacea - Google Patents
Method for restoring heavy metal in sewage irrigation soil through NTA and 3-indoleacelic acid-enhanced festuca arundinacea Download PDFInfo
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Abstract
The invention discloses a method for restoring heavy metal in sewage irrigation soil through NTA and 3-indoleacelic acid (IAA)-enhanced festuca arundinacea. A mixture comprises chelating agent nitrilotriacetic acid and 3-indoleacelic acid in a mole ratio of 6-12mmol.kg＜-1＞ to 2mg.kg＜-1＞. Experimental results show that under certain concentration, the plant toxicity of heavy metal and the chelating agent on festuca arundinacea can be relieved through the addition of IAA, and the accumulation of the plant on the heavy metal can be facilitated; the improvement of the above-ground biomass of the festuca arundinacea can be promoted through combination of the NTA and 3-indoleacelic acid. By comparing the combination of the IAA and NTA with the control group, the content of above-ground heavy metal of the festuca arundinacea can be increased, and therefore, more heavy metal can be extracted and accumulated by lawn plant, and the heavy metal-polluted soil can be restored to the maximum extent.
Description
Technical field
The invention belongs to environmental protection technical field, relate to a kind of NTA of employing and the enrichment of 3-indolyl acetic acid strengthening Festuca ArundinaceaThe method of dirty Irrigated Area Soils heavy metal.
Background technology
China's Proposals of Water Resources various pollution problems, water resource lack become restriction China economic development important because ofOne of element. Reasonably use sewage irrigation can alleviate the problem of water resource anxiety, can also saving water resource, alleviate environmentPollute. Dirty filling refers to be carried out after harmless treatment city domestic sewage and industrial wastewater, directly or indirectly for farmland sewageIrrigation, gardens sewage irrigation and groundwater reservoir recharge. China starts to build sewage irrigation engineering in nineteen fifty-seven after the founding of the state, goes through so farTime over half a century, China has also passed through relevant dirt and has filled with principle, policy and standard during this period.
The sewage irrigation of China is broadly divided into 3 stages: nineteen fifty-seven was spontaneous use sewage irrigation period in the past, from 20The forties in century, near Beijing, start someone and utilize waste water irrigation farmland; Nineteen fifty-seven sewage irrigation is put into national sectionGrind plan, obtained initial development to dirty filling of China during 1972; So far sewage irrigation in 1972 develops rapidly. From the sixtiesJust, the dirty area of filling with of China expands rapidly, northern combined task distributed Beijing, Tianjin, Baoding, Shenyang, Shijiazhuang, ZhengzhouEtc. famous dirty irrigated area, these areas account for dirty the more than 90% of area of filling with in the whole nation, and the dirt of south and highlands filling area is relativeLess. Because sewage treatment facility efficiency is low, somewhat expensive etc., a large amount of raw sewerages are directly irrigated agriculture as water sourceField.
Also there are a lot of countries and regions to adopt sewage to irrigate farmland abroad. The U.S. utilizes sewage early in the worldCountry, at present the whole America municipal sewage accounts for 60% of total amount for what irrigate, the handling rate of municipal sewage reaches 100%. Japan fromEarly 1960s just starts to reclaim sewage, at present first by after sewage disposal, introduces farmland after making indices up to standard again and entersRow is irrigated. Extremely Israel's wastewater treatment rate of lack of water has reached more than 90%, and utilization rate also has 70%, wherein 57% sewage purificationAfterwards for watering agricultural and meadow, gardens. France is used a century around of sewage irrigation, and government opens energetically on the one handExhibition sewage disposal research, government takes much count of the impact of sewage irrigation on agricultural byproducts quality on the other hand, has set up specially dirty fillingHealth guidance association, regularly publishes relevant information and sewage irrigation situation is exercised supervision, and government also instructs people by inhomogeneityThe irrigation of classifying of the sewage of type. The history of Germany's sewage irrigation has more than 100 year, and surrounding area, Berlin city was just set up in nineteen twenty-eight12 Ge Wuguan farms, area reaches 10,000 hm2. Although Berlin city has now built complete urban sewage treatment system, these sewageIrrigate farm or an important component part as municipal sewage treatment. Also have the states such as Brazil, Argentina, Greece, ChileFamily also has a large amount of practical experiences at sewage aspect utilizing.
The shortage of water resource has been alleviated in sewage irrigation to a certain extent, still fills with the expansion of scale along with dirt, to soilImpact more and more obvious. Change physicochemical property, Organic Pollution and the heavy metal pollution of soil. Sewage irrigation is a soil huge sum of moneyOne of the important sources belonging to, have in 7 heavy metal higher than background value in the dirty Irrigated Area Soils of the cool phoenix in Beijing, and have and increase graduallyTrend; The dirty Irrigated Area Soils in Baiyin city is subject to heavy metal ion severe contamination, need to pass through many kinds of measures comprehensive regulation abilityPollution remission situation.
In sewage, contain a large amount of heavy metal elements, in soil, accumulation can affect absorption and the utilization of plant to nutrient,Also the metabolic balance that can upset plant carries out field experiment in Beijing suburb, result show dirty fill with affected the plant height of summer corn,Leaf area index, obviously reduces its output and dry. The pollution main manifestations of dirty irrigated area crop quality is heavy in agricultural productThe change of overrun metal content and nutritional labeling. There are some researches show, use each index of quality of sewage irrigation mud field riceAll irrigate the obvious decline that has in mud field than clear water. Also there is research to detect the terraneous potato of soil, the beet etc. of sewage irrigationAgricultural product, result shows that wherein in Cd content and the soil liquid, Cd content is linear correlation.
Agriculture environmental protection place is investigated the agricultural product of more than 300 contaminated area of 24 provinces and cities in the whole nation for 1997, knotFruit shows that wheat, corn heavy metals exceeding standard rate are 15.5%, 14.0%, and heavy metal pollution is mainly taking mercury, chromium, cadmium, arsenic etc. as main.Sewage irrigation also can make the protein content of wheat and paddy rice reduce, and along with the prolongation of sewage irrigation duration, the quality of crop can be year by yearDecline. Dirty filling also can significantly be reduced Vitamin C content in vegetables, and the content of other nutritional labelings is to have to increase to have to subtract.
Heavy metal in sewage is mainly enriched between the 0-20 cm of dirty Irrigated Area Soils top layer. The absorption of soil to heavy metalReduce heavy metal to underground migration. There are some researches show long-term dirty area of filling with, local underground water is not subject to heavy metalPollution, in most cases, the heavy metal of dirt in pouring water can't significantly affect underground water generates. Also there are some researches show,Heavy metal in soil especially Cd can show with the increase of leaching time the trend of downward migration, thereby underground water is madeBecome to threaten. In a word, in dirty sewage of filling with, some pollutant is by adsorption by soil, and another part can move to soil lower floorMoving, finally phreatic water is affected.
Sewage irrigation causes soil and pollution of agricultural products on the one hand, makes pollutant in crop or the middle accumulation of winning, soEnter people by food chain afterwards, then cause the incidence of disease of various chronic diseases to rise; Long-term dirt filling can cause on the other handGroundwater body pollution, also can endanger human and livestock health after the daily drinking-water of people or livestock consumption; Also have long sewage irrigation,Peasant's health on direct contact also has impact. As the Shaoguan in the Ganzhou in Jiangxi and Dayu and Guangdong and the Qujiang River, lake19 areas such as south, Shaanxi have indivedual areas to produce the situation of " cadmium rice ", if eat for a long time such agricultural product, and can be to peopleHealthy working the mischief.
Owing to directly irrigating with a large amount of raw sewerages, overproof water quality, the dirty area of filling with blindly expands again, causes soilWith the pollution of crop and underground water, sewage irrigation has been the one of the main reasons that causes China's Rural Water Environment to worsen. SewageIrrigating major part is that peasant is spontaneous, and their majority is in the situation that can not get clear water, spontaneous introducing urban life and workIndustry waste water is as iirigation water source, and this suburb nearby in northern China big and medium-sized cities is very general.
Traditional heavy metal pollution of soil recovery technique has casting landfill method, dilution method, elution method, physical partition method and changeMethod etc. Compared with traditional processing mode, the major advantage of phytoremediation is that cost is low, and treatment facility is simple, is applicable to large ruleThe application of mould, is beneficial to the maintenance of soil ecosystem, little to environmental perturbation, and the feature such as possess aesthetic value.
In the biological restoration of heavy metal pollution of soil, application is phytoremediation technology early, more widely. PhytoremediationUtilize exactly the heavy metal contaminants in plant absorption, accumulation and fixing soil, transferred to root or aerial part, thenGather in the crops at suitable growth phase, after treatment, ashing is reclaimed heavy metal or is carried out landfill. Chen Tongbins etc. are reported for work firstCiliate desert-grass can a large amount of enrichment As result of study, analyzed the enriching quantity of this plant Different Organs to heavy metal simultaneously, findIn ciliate desert-grass Different Organs tissue, the content of As is accessory pinna > petiole > root system, illustrate As in this plant easily upwards transport andEnrichment, demonstrating ciliate desert-grass has extremely strong patience and unique accumulation ability to As. Sun etc. study discovery, are grown in the ore deposit containing CdIn Sedum alfredii Hance plants stems in dregs earth and leaf, Cd content is apparently higher than root, and the Sedum alfredii Hance that is grown in non-Cd mining area is plantedStrain root Cd content is apparently higher than the content in cauline leaf, thereby confirms that Sedum alfredii Hance also has stronger enrichment to Cd. FieldVictory Buddhist nuns etc. are by being preced with relatively thinking of grass with goose, and vetiver is that Cu, Pb, Zn Single Pollution or combined pollution are all hadGood repair function. Ye Chun and studied reparation and the activating mechanism of alfalfa to Pb contaminated soil, from X-ray micro-zone analysisResult finds out, space between cells Pb content is the highest, and cell membrane and vacuole take second place, minimum in kytoplasm; Pb is main in alfalfa bodyForm with indissoluble exists, and alfalfa is relevant with the formation of plant complexing element to the tolerance of Pb. Due to biomass of alfalfaHeight, so it is the desirable rehabilitation plant of one that P in soil b pollutes that alfalfa can be used as. Secretions from plant roots can change soilEarth rhizospheric environment, can make Cr, the Hg of multivalence state, valence state and the form of As change, and affects its poisonous effect. The root of plantHair can directly increase root table from soil exchange adsorption heavy metal to be fixed. But, the surpassing for restoration of soil polluted by heavy metal havingThe growth cycle of enriching plant is grown, biomass is relatively little, low for the bioaccumulation efficiency of the low heavy metal of some biological effectiveness,This is restricted single use phytoremediation.
In reality, pollutant in soil is of a great variety, and combined pollution is general, and pollution level and difference in thickness are large, simultaneously the earthThe soil types on top layer is many, and the area differentiation of its composition, character, condition is obvious, and repairs the space of rear soil recycling formulaPlanning requirement difference. Therefore, individual event recovery technique is toward being difficult to reach reparation target, and exploitation complex repairation pattern just becomes soilPollute the main direction of studying of repairing.
The main Types that starts now the complex repairation technology that drops into application has the reparation of plant/microbial association, animal/plantingThing is combined reparation and chemistry/materialization-Co-bioremediation. The mechanism of plant/microbial association reparation is higher plant and soilOften there is synergy in earth microorganism, on the one hand plant can provide the edaphon required carbon of growing in growth courseSource, simultaneously again through base of leaf to root delivering oxygen, be formed with the microenvironment that is beneficial to oxidation, promote aerobic microbiological to pollutantDecomposition. Chemistry/materialization-Co-bioremediation can be brought into play the advantage of short, instant effect of the cycle that chemistry or physics repairs, knotClose nondestructive biological restoration feature, development is that contaminated soil the most with potential applications is repaiied based on chemical-biological recovery techniqueCompound method. As utilize the coordination stripping of organic complexing agent, and increase heavy metal concentration in the soil liquid, improve the absorption effect of plantRate, thus realize the reparation of strengthening Enhanced phytoextraction. Therefore, improve plant and absorb the method for enriching heavy metal, except using andBeyond the super enriching plant that continuation is found accumulation ability by force and biomass is large, we can add manually in phytoremediation processOr natural chelating agent and other enhancements improve the ability of plant absorption enriching heavy metal.
Chelating agent inducing plant extracts remediating heavy metal, and it can increase the solubility of heavy metal in soil on the one hand,Can improve on the other hand heavy metal rhizosphere diffusivity, can also promote heavy metal to transport to overground part from root system. Pealosa etc.Studied the factor that several promotion lupin repairing heavy metal in soil pollute, found that, chelating agent NTA can promote metal fromSon (Fe, Mn, Cu, Zn, Cd) migration, impels the contained concentration of metal ions of lupin to raise, especially As, Cd and Pb concentrationIncrease more obvious. Qian Meng etc. plant elsholtzia splendens on Cu, Zn, Pb, Cd combined contamination soil, use 5 mmolkg-1EDTAAfter, the concentration of Cu, Zn, Pb, Cd improves 27.4,3.2,17.6 and 89.5 times than contrast respectively. The researchs such as Stanhope show,Along with the increase of EDTA concentration, in soil, the content of Cu, Zn, Cd, Pb, Ni soluble state heavy metal accounts for total content of beary metalRatio is linear raising. In chelation evoked phytoremediation process the service time of chelating agent very important, be generally to gather in the crops plantIn first 1 ~ 2 week, add, now growth and development of plants maturation, less on increment impact after processing in the short time, plant extractTotal metals can significantly increase.
By warmhouse booth potted plant experiment, research chelating agent and surfactant is independent or the auxiliary Jin Fugu of Combined Processing repaiiesThe effect of multiple heavy-metal contaminated soil. Result shows, the EDTA of high concentration makes the biomass of Jin Fugu reduce than contrast, fructificationThe concentration of Pb, Cu and Cd improves 15 ~ 88 times, 0.8 ~ 3.3 times and 0.5 ~ 0.6 times than contrast respectively. add separately surfactantTime, respectively processing biomass does not have significant difference with contrasting, and heavy metal concentration amplitude of variation does not have to add separately the processing of EDTAGreatly. But this conventional complexing agent of EDTA has stronger bio-toxicity, in application, be greatly limited.
Plant hormone heteroauxin (IAA) is alleviated the coercion of heavy metal on plants. If IAA can be alleviated heavy metalThe phytotoxicity of chelating agent, promotes root system of plant to extend, and increases phytomass, utilizes so IAA to work in coordination with chelating agent plant pairAbsorption, transhipment and the accumulation of heavy metal, if can improve plant extract efficiency, will have great importance. But it is heavy to utilize IAA to repairThe correlation technique of metal there is no bibliographical information.
Lawn vegetation is checked winds and fixed drifting sand, water conservation, purify air, afforest soil, conserve water and soil and regulate miniclimate etc.Effect. Lawn plant has certain using value in addition, and it is the important component part of urban landscaping, can be used for afforestingCity, and its adaptability is stronger. If being applied to heavy metal-polluted soil reparation, lawn plant both can avoid food chain, withTime can also take away the heavy metal in soil, and turf volume can be used further to the greening in city. If lawn plant is applied toThe native heavy metal reparation of dirty filling, has certain reparation value and practical value.
In contaminated soil, most of heavy metal ion, in solid phase, are attracted to soil particle surface, and knotThat closes is very firm. And chemical method can be broken this state, by being converted in the heavy metal of solid phase, plant can enrichmentIn the metal ion of liquid phase. The combination principle of phytoremediation and chemical method be exactly disturbance heavy metal in liquid phase of soil concentration andBalance between solid concentration. Therefore,, for many plants, need in soil, add mobile agent to increase in the soil liquidMetal concentration, and then promote absorption and the enrichment of plant to metal. Heteroauxin can be alleviated the plant poison of heavy metal chelantProperty, promote root system of plant to extend, increase phytomass, collaborative chelating agent promote the absorption of plant to heavy metal, transhipment andAccumulation, significantly improves plant extract efficiency. Select the plant with certain Heavy Metal Tolerance to carry out planting of heavy-metal contaminated soilThing is repaired, and both can complete the recycling of contaminated soil, can avoid again pollutant to transmit by food chain, so have important reasonOpinion meaning and practical value. There are some researches show, lawn plant has certain absorption and tolerance to heavy metal-polluted soil. And at presentNTA is combined to lawn plant repairs the technology of dirty Irrigated Area Soils heavy metal and also there is no bibliographical information with 3-indolyl acetic acid.
Summary of the invention
The object of the present invention is to provide a kind of NTA of employing and the dirty irrigated area of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichmentThe method of heavy metal-polluted soil. The present invention, on the basis of Optimal Experimental, proves: lawn plant just can be drawn accumulation fund by experimentMore heavy metal, the to the full extent soil of repairing heavy metal pollution. For realizing this object, the invention provides following skillArt scheme:
A kind of NTA and 3-indolyl acetic acid mixture, is characterized in that it is by chelating agent nitrilotriacetic acid and 3-indolyl acetic acidComposition, wherein the mol ratio of chelating agent nitrilotriacetic acid and 3-indolyl acetic acid is 6-12mmolkg-1NTA:3-heteroauxin 2mg
kg-1。
The present invention further discloses NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichmentMethod, it is characterized in that being undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (Festuca arundinacea L.) be experiment material;
Chelating agent is selected nitrilotriacetic acid; 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, will after the soil removal grass roots of collection, stone, divide in transparentOn Polypropylence Sheet, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use. Soil organic matter content 3.62%, total nitrogen content0.19%, full phosphorus amount 5.4 gkg-1, full potassium amount 787.3 mgkg-1, pH7.28, soil moisture content 4.13%, electrical conductivity 0.44
ms·cm-1. In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg-1, be respectively soil ring23.8,1.5 and 3.2 times of border quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment4)2SO4、Na2SO4、K2SO4、MgSO4、Ca(NO3)2、Mg(NO3)2、H2SO4Make SO4 2-、NO3 -、Cl-、NH4 +、Mg2+、Ca2+、K+、Na+Concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL-1'sRainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, cotton and nylon wire sealing for bottom, then existIn each pipe, plant 0.3g Festuca Arundinacea grass seeds, pvc pipe is sling and fixed, lower end is joined and is put conical flask and access effusion. LawnPlant is cultivated and carries out in laboratory, and often reversing of position is consistent to ensure illumination, 18 ~ 35 DEG C of indoor temperatures between culture period, phaseTo humidity 40 ~ 61%, normal water supply between establishment period, to maintain plant normal growth. Plant growth 30d, according to experimental design respectivelyBy the complex repairation agent of respective amount, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast,After processing 10d, gather in the crops lawn plant aerial part;
(3) mensuration of biomass: after sowing 40d, gather in the crops lawn plant aerial part, after deionized water is rinsed repeatedly, willIt puts into baking oven, under 80 DEG C of conditions, dries to constant weight, surveys its dry weight, finally surveys with TAS-990 atomic absorption spectrophotometerDetermine content of beary metal in digestive juice; Wherein the NTA of respective concentration or 3-indolyl acetic acid refer to wherein one below:
6 mmol·kg-1NTA；
12 mmol·kg-1NTA；
6 mmol·kg-1NTA+3-indolyl acetic acid 2mgkg-1；
12 mmol·kg-1NTA+ 3-indolyl acetic acid 2mgkg-1。
The present invention further discloses NTA and combine the dirty Irrigated Area Soils heavy metal of Festuca Arundinacea enrichment with 3-indolyl acetic acid mixtureThe application of method aspect the dirty Irrigated Area Soils heavy metal of enrichment. Described Festuca Arundinacea enriching heavy metal refers to enrichment Festuca ArundinaceaThe content of middle Cd.
The present invention further discloses NTA and the dirty Irrigated Area Soils huge sum of money of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichmentThe application of the method belonging to aspect the soil of repairing heavy metal pollution. Wherein said complex repairation agent refers to 6 mmol
kg-1NTA+heteroauxin 2mgkg-1。
Test of the present invention shows: IAA combines interpolation compared with control group with NTA, has improved Festuca Arundinacea heavy metal on the groundContent, therefore, lawn plant just can draw accumulation fund more heavy metal, the to the full extent soil of repairing heavy metal pollutionEarth.
The more detailed preparation method of the present invention is as follows:
1 development materials and methods
1.1 experiment material
Select the more common perennial Festuca Arundinacea of northern China (Festuca arundinacea L.) be experiment material.
Chelating agent is selected nitrilotriacetic acid (NTA), and molecular formula is N (CH2COOH)3, be purchased from the limited public affairs of traditional Chinese medicines group chemical reagentDepartment, pure for analyzing.
3-indolyl acetic acid molecular formula is C10H9NO2, be purchased from Tianjin recovery fine chemistry industry research institute, pure for analyzing.
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, will after the soil removal grass roots of collection, stone, divide in transparentOn Polypropylence Sheet, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use. Soil organic matter content 3.62%, total nitrogen content0.19%, full phosphorus amount 5.4 gkg-1, full potassium amount 787.3 mgkg-1, pH7.28, soil moisture content 4.13%, electrical conductivity 0.44
ms·cm-1. In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg-1, be respectively soil ring23.8,1.5 and 3.2 times of border quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment4)2SO4、Na2SO4、K2SO4、MgSO4、Ca(NO3)2、Mg(NO3)2、H2SO4Make SO4 2-、NO3 -、Cl-、NH+、Mg2+、Ca2+、K+、Na+Concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL-1'sRainwater, and with HCl allotment pH be 5.59.
1.2 experimental design
This experiment arranges 5 processing altogether: do not add the contrast (I) of NTA and 3-indolyl acetic acid; 6 mmolkg-1NTA（II）；
12 mmol·kg-1NTA（III）；6 mmol·kg-1NTA+3-indolyl acetic acid 2mgkg-1（IV）；12 mmol·kg-1NTA+ 3-indolyl acetic acid 2mgkg-1(V). Each processing repeats for 3 times.
1.3 lawn plants are cultivated
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, cotton and nylon wire sealing for bottom, then existIn each pipe, plant 0.3g Festuca Arundinacea grass seeds, pvc pipe is sling and fixed, lower end is joined and is put conical flask and access effusion. LawnPlant is cultivated and carries out in laboratory, and often reversing of position is consistent to ensure illumination, 18 ~ 35 DEG C of indoor temperatures between culture period, phaseTo humidity 40 ~ 61%. Normal water supply between establishment period, to maintain plant normal growth. Plant growth 30d, according to experimental design respectivelyThe NTA of respective concentration is dissolved in to distilled water by required dosage, and 3-indolyl acetic acid is dissolved in a small amount of ethanol by required dosage, disposable executingBe added on the stromal surface of respective handling group, the distilled water pouring of equivalent for contrast. After processing 10d, gather in the crops lawn plant overground partPoint.
1.4 index determining
The mensuration of biomass: gather in the crops lawn plant aerial part after sowing 40d, after deionized water is rinsed repeatedly, by itPut into baking oven, under 80 DEG C of conditions, dry to constant weight, survey its dry weight.
The mensuration of content of beary metal: weigh plant sample 0.1 g, with nitric acid: perchloric acid: sulfuric acid (8:1:1) is cleared upAfter, gained solution is settled to 25mL with distilled water. Finally measure a huge sum of money in digestive juice with TAS-990 atomic absorption spectrophotometerBelong to (Cd, Cu, Zn) content.
1.5 data analysis processing
Adopt SPSS17.0 software to carry out statistical analysis to data.
2 development results analyses
The impact on Festuca Arundinacea ground biomass of 2.1 NTA and 3-indolyl acetic acid
Under different disposal, the overground part of Festuca Arundinacea is in table 1. 12 mmolkg-1Festuca Arundinacea ground biomass in NTA processed groupThan contrast reduced by 4.35%, but with contrast difference not obvious (P> 0.05). Only have 6 mmolkg-1NTA+3-indolesThe Festuca Arundinacea ground biomass of acetic acid treatment is significantly higher than contrast, has improved 20.87%, but adds two places of heteroauxinReason group difference is not remarkable. NTA and heteroauxin are combined compared with interpolation group adds separately NTA processed group with same concentration, Festuca ArundinaceaGround biomass increases, particularly 6 mmolkg-1NTA+heteroauxin processed group is than 6 mmolkg-1NTA processesGroup has significantly improved 19.83%.
The impact of table 1 different disposal on Festuca Arundinacea ground biomass
| Process | Ground biomass (g/PVC pipe) |
| Do not add NTA and 3-indolyl acetic acid | 0.115±0.012b |
| 6 mmol·kg-1NTA | 0.116±0.015b |
| 12 mmol·kg-1NTA | 0.110±0.008b |
| 6 mmol·kg-1NTA+3-indolyl acetic acid | 0.139±0.006a |
| 12 mmol·kg-1NTA+3-indolyl acetic acid | 0.128±0.010ab |
With the different letter representation significant differences of column data (P <0.05）
The collaborative impact on Festuca Arundinacea overground part content of beary metal of 2.2 NTA and 3-indolyl acetic acid
Festuca Arundinacea overground part content of beary metal is as table 2. Totally it seems and add after NTA, Festuca Arundinacea overground part Cd, Cu, Zn'sContent (6 mmolkg that are significantly increased compared with the control-1Except NTA processed group Cd content). 6 mmolkg-1NTA+YinIndolylbutyric acid processed group Festuca Arundinacea is Cd content maximum on the ground, has significantly improved 114%, and be also significantly higher than 6 mmol than contrast
kg-1NTA processed group. Add separately compared with the content of NTA group and Festuca Arundinacea Cu, Zn in same concentration NTA+ heteroauxin group difference equalNot significantly (P> 0.05). Illustrate that NTA combines interpolation raising Festuca Arundinacea Cu, Zn content are not had to remarkable result with heteroauxin.
Festuca Arundinacea overground part content of beary metal (ug/g) under table 2 different disposal
| Process | Cd | Cu | Zn |
| Do not add NTA and 3-indolyl acetic acid | 1.867±0.252b | 5.867±0.551c | 6.133±0.839d |
| 6 mmol·kg-1NTA | 2.467±0.416b | 8.767±1.007ab | 16.700±3.869bc |
| 12 mmol·kg-1NTA | 3.867±0.321a | 10.833±1.662a | 22.933±3.729a |
| 6 mmol·kg-1NTA+3-indolyl acetic acid | 4.000±0.700a | 8.233±1.137b | 14.167±2.050c |
| 12 mmol·kg-1NTA+3-indolyl acetic acid | 3.967±0.702a | 10.433±0.777a | 20.367±1.762ab |
With the different letter representation significant differences of column data (P <0.05）
3 development conclusions
Under finite concentration, the interpolation of IAA can be alleviated heavy metal and the phytotoxicity of chelating agent to Festuca Arundinacea, is conducive to plantThe accumulation of thing to heavy metal; Add that NTA combines interpolation with 3-indolyl acetic acid and can promote the raising of Festuca Arundinacea ground biomass.IAA combines interpolation compared with control group with NTA, improved Festuca Arundinacea content of beary metal on the ground, and therefore, lawn plant just can be carriedGet accumulation more heavy metal, the to the full extent soil of repairing heavy metal pollution.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention will be further described, and following each embodiment is only for illustrating the present inventionNot limitation of the present invention. Wherein chemical reagent used all has commercially available.
Embodiment 1
The method of NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichment:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (Festuca arundinacea L.) be experiment material;
Chelating agent is selected nitrilotriacetic acid; 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, will after the soil removal grass roots of collection, stone, divide in transparentOn Polypropylence Sheet, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use. Soil organic matter content 3.62%, total nitrogen content0.19%, full phosphorus amount 5.4 gkg-1, full potassium amount 787.3 mgkg-1, pH7.28, soil moisture content 4.13%, electrical conductivity 0.44
ms·cm-1. In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg-1, be respectively soil ring23.8,1.5 and 3.2 times of border quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment4)2SO4、Na2SO4、K2SO4、MgSO4、Ca(NO3)2、Mg(NO3)2、H2SO4Make SO4 2-、NO3 -、Cl-、NH4 +、Mg2+、Ca2+、K+、Na+Concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL-1'sRainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, cotton and nylon wire sealing for bottom, then existIn each pipe, plant 0.3g Festuca Arundinacea grass seeds, pvc pipe is sling and fixed, lower end is joined and is put conical flask and access effusion. LawnPlant is cultivated and carries out in laboratory, and often reversing of position is consistent to ensure illumination, and between culture period, 18 DEG C of indoor temperatures, relatively wetDegree 40%, normal water supply between establishment period, to maintain plant normal growth. Plant growth 30d, shines experimental design respectively by respective amountComplex repairation agent, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, processes 10dRear results lawn plant aerial part;
(3) mensuration of biomass: after sowing 40d, gather in the crops lawn plant aerial part, after deionized water is rinsed repeatedly, willIt puts into baking oven, under 80 DEG C of conditions, dries to constant weight, surveys its dry weight, finally surveys with TAS-990 atomic absorption spectrophotometerDetermine heavy metal Cd in digestive juice, Cu, Zn content; Wherein respective concentration: 6 mmolkg-1NTA+3-indolyl acetic acid 2mgkg-1。
Embodiment 2
The method of NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichment:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (Festuca arundinacea L.) be experiment material;
Chelating agent is selected nitrilotriacetic acid; 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, will after the soil removal grass roots of collection, stone, divide in transparentOn Polypropylence Sheet, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use. Soil organic matter content 3.62%, total nitrogen content0.19%, full phosphorus amount 5.4 gkg-1, full potassium amount 787.3 mgkg-1, pH7.28, soil moisture content 4.13%, electrical conductivity 0.44
ms·cm-1. In soil, the content of Cd, Cu and Zn is respectively 7.13,146.31 and 795.56 mgkg-1, be respectively soil ring23.8,1.5 and 3.2 times of border quality secondary standard (GB156182-1995).
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation.(NH for this experiment4)2SO4、Na2SO4、K2SO4、MgSO4、Ca(NO3)2、Mg(NO3)2、H2SO4Make SO4 2-、NO3 -、Cl-、NH4 +、Mg2+、Ca2+、K+、Na+Concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78 mgL-1'sRainwater, and with HCl allotment pH be 5.59.
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, cotton and nylon wire sealing for bottom, then existIn each pipe, plant 0.3g Festuca Arundinacea grass seeds, pvc pipe is sling and fixed, lower end is joined and is put conical flask and access effusion. LawnPlant is cultivated and carries out in laboratory, and often reversing of position is consistent to ensure illumination, 18 ~ 35 DEG C of indoor temperatures between culture period, phaseTo humidity 50%, normal water supply between establishment period, to maintain plant normal growth. Plant growth 30d, takes experimental design respectively by pictureThe complex repairation agent that should measure, the disposable stromal surface that puts on respective handling group, the distilled water pouring of equivalent for contrast, processesAfter 10d, gather in the crops lawn plant aerial part;
(3) mensuration of biomass: after sowing 40d, gather in the crops lawn plant aerial part, after deionized water is rinsed repeatedly, willIt puts into baking oven, under 80 DEG C of conditions, dries to constant weight, surveys its dry weight, finally surveys with TAS-990 atomic absorption spectrophotometerDetermine heavy metal Cd in digestive juice, Cu, Zn content; Wherein respective concentration: 12 mmolkg-1NTA+ 3-indolyl acetic acid 2mgkg-1。
Claims (3)
1. adopt a method for NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichment, its spyLevy and be that it is made up of chelating agent nitrilotriacetic acid and 3-indolyl acetic acid, wherein chelating agent nitrilotriacetic acid is 6-12mmolkg- 1NTA, 3-indolyl acetic acid is 3-indolyl acetic acid 2mgkg-1; And undertaken by following step:
(1) processing of material:
Select the more common perennial Festuca Arundinacea of northern China (Festuca arundinacea L.) be experiment material;
Chelating agent is selected nitrilotriacetic acid; 3-indolyl acetic acid
Take from dirty irrigated area, Xiqing District, Tianjin for examination soil, will after the soil removal grass roots of collection, stone, divide in transparent plasticOn cloth, be positioned over ventilation, after natural air drying 2 ~ 3 d, cross 2 mm sieves for subsequent use;
The preparation of artificial rainwater: being 5.59 according to the Tianjin whole city precipitation pH annual mean of monitoring out, is acid precipitation;
(NH for this experiment4)2SO4、Na2SO4、K2SO4、MgSO4、Ca(NO3)2、Mg(NO3)2、H2SO4Make SO4 2-、NO3 -、
Cl-、NH4 +、Mg2+、Ca2+、K+、Na+Concentration is respectively 14.96,6.54,1.68,3.71,0.82,1.38,0.64 and 0.78
mg·L-1Rainwater, and with HCl allotment pH be 5.59;
(2) experimental technique:
At high 25cm, in the pvc pipe of diameter 3cm, add the dirty native 170g of filling, cotton and nylon wire sealing for bottom, then eachIn pipe, plant 0.3g Festuca Arundinacea grass seeds, pvc pipe is sling and fixed, lower end is joined and is put conical flask and access effusion;
Lawn plant is cultivated and carries out in laboratory, and often reversing of position is consistent to ensure illumination, indoor temperature 18 between culture period ~35 DEG C, relative humidity 40 ~ 61%, normal water supply between establishment period, to maintain plant normal growth;
Plant growth 30d, according to experimental design respectively by the NTA of respective amount and 3-indolyl acetic acid, the disposable corresponding position that puts onThe stromal surface of reason group, the distilled water pouring of equivalent for contrast, gathers in the crops lawn plant aerial part after processing 10d;
(3) mensuration of biomass: gather in the crops lawn plant aerial part after sowing 40d, after deionized water is rinsed repeatedly, putEnter in baking oven, under 80 DEG C of conditions, dry to constant weight, survey its dry weight, then weigh above-mentioned plant sample 0.1 g, with mass ratio be 8:The nitric acid of 1:1: perchloric acid: after Sulfuric-Acid Digestion, gained solution is settled to 25mL with distilled water, finally inhales with TAS-990 atomReceive content of beary metal in the above-mentioned digestive juice of spectrophotometric determination.
2. the method for NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichment described in claim 1,Heavy metal wherein refers to Cd, Cu, Zn.
3. the method for NTA and the dirty Irrigated Area Soils heavy metal of 3-indolyl acetic acid mixture strengthening Festuca Arundinacea enrichment described in claim 1Application aspect the soil of repairing heavy metal pollution; Wherein said NTA and 3-indolyl acetic acid consumption are 6 mmolkg- 1NTA+heteroauxin 2mgkg-1。
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