CN103859141A - Physical modification preparation method of high-solubility rice protein - Google Patents
Physical modification preparation method of high-solubility rice protein Download PDFInfo
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Abstract
The invention discloses a physical modification preparation method of high-solubility rice protein. The method comprises the steps of dispersing raw material rice protein into the water, processing through alkaline liquid, freezing, crushing, neutralizing through acid, centrifuging, taking the liquid supernatant, and atomizing drying, so that a finished product of high-solubility rice protein can be obtained. The rice protein product with high solubility can be prepared by utilizing the method, the protein solubility is more than or equal to 98 percent, the emulsion performance is more than or equal to 0.50, the bubbling performance is more than or equal to 17.39, the utilization rate and additional value of the rice protein can be greatly improved, and the application prospect is wide.
Description
Technical field
The invention belongs to rice protein finishing technology field, be specifically related to a kind of physical modification preparation method of high dissolubility rice protein and the soluble rice protein that the method prepares.
Background technology
Rice is China's staple food grain.The protein efficiency value (PER) of rice protein is between 2.0 and 2.5, digestibility is more than 90%, meanwhile, the characteristic such as the reduced immunogenicity of rice protein, antitumor, anti-hypertension, anti-angiogenic atherosis and anti-cholesterol has been proved, has very wide application prospect.Its not main cause of broad commercial applications but rice protein is poorly soluble always.Dissolubility is the important indicator of the processing of measurement product protein and application performance, is determining the critical function such as emulsibility and foaming characteristic characteristic.
Improve at present the deliquescent method of rice protein and mainly contain chemical method and enzyme process: chemical method is structure, electrostatic charge and the hydrophobic group by changing protein, thereby improve its dissolution properties, but the external source group that chemical modification is introduced has changed original composition of rice protein, cause nutrient protein loss; Enzyme process is to utilize protease protein molecular to be carried out to catalyzing hydrolysis under certain condition, make protein macromolecule be degraded into the little molecule of different chain length, protein after enzyme hydrolysis, except its functional character obviously changes, the peptide class that enzymolysis the generates digestion that is more easily absorbed by the body, but hydrolysis degree is wayward, and cost is relatively high, if hydrolysis degree is less, performance improvement is not obvious, if hydrolysis degree is larger, the peptide class of generation makes product with bitter taste.Physical method is also used to the solubilising modification of protein, such as processing methods such as ultrasonic, super-pressure, jet cooking method, microjet methods, but these methods for rice protein solubility to improve effect quite limited, be not suitable for suitability for industrialized production.
Summary of the invention
The above-mentioned defect existing for the existing rice protein method that has improved solubility, the applicant is through Improvement, a kind of physical modification preparation method of high dissolubility rice protein is provided, rice protein dissolubility >=98% preparing by this method, emulsibility >=0.50, foaming characteristic >=17.39, have improved utilization rate and the added value of rice protein greatly.
Technical scheme of the present invention is as follows:
A physical modification preparation method for high dissolubility rice protein, is scattered in Raw Materials Rice albumen in water, through alkali lye processing, freezing, pulverize, and acid neutralization, centrifuging and taking supernatant, spraying is dry, obtains high dissolubility rice protein finished product.
Preferably, the inventive method comprises the following steps:
(1) disperse and alkali treatment: rice protein is scattered in water, and solid-liquid ratio 1:15～25, stir and evenly mix, and above-mentioned rice protein dispersion liquid pH value are adjusted to 10.5～12.5 with NaOH solution, are preferably pH12.5;
(2) freezing: step (1) gained feed liquid is carried out in-20～-10 ℃ freezing, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until this solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0～7.5 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 10～20min of 1000g, is collected to supernatant;
(6) repeat: (5) gained sediment is pressed to step (1)～(5) reprocessing 2～3 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, obtain high dissolubility rice protein finished product.Particularly, 110～140 ℃ of the described spray drying control system spray dryer of step (7) inlet temperatures, 80～100 ℃ of outlet temperatures.
The high dissolubility rice protein preparing by the inventive method is with a wide range of applications, and can apply to food, medicine, cosmetics and high-end health products trade.
For the rice protein that adopts the inventive method to prepare, adopt Kjeldahl's method method to measure protein recovery and dissolubility, adopt turbidity method to measure emulsibility, adopt expansion rate method to measure foaming characteristic, concrete assay method is as follows:
(1) product protein recovery
According to the present invention, protein recovery is construed as albumen quality in soluble rice protein product and accounts for the percentage of albumen quality in Raw Materials Rice albumen.
Albumen quality m after dry with Kjeldahl nitrogen determination spraying in soluble rice protein product and the albumen quality m0 in material protein.
The protein recovery of soluble rice protein represents with following formula:
Protein recovery (%)=(m/m
0) × 100
In formula: m---the albumen quality after spraying is dry in soluble rice protein product;
M
0---albumen quality in material protein.
(2) dissolubility
According to the present invention, dissolubility is construed as in the soluble rice protein product of per unit mass, and soluble albumen quality accounts for the percentage of albumen quality in product.
50mmol/L potassium dihydrogen phosphate-sodium hydrate buffer solution (pH7.0) for soluble rice protein sample is made into 1%(W/V) solution, at 25 ℃, stir 1h, the centrifugal 10min of 3000r/min, then measure respectively the albumen quality m in albumen quality m1 and the soluble rice protein product in supernatant with Kjeldahl's method.
Soluble rice protein solubility represents with following formula:
Solubility (%)=(m of protein
1/ m) × 100
In formula: m
1---albumen quality in supernatant;
M---the albumen quality in soluble rice protein product.
(3) emulsibility
According to the present invention, emulsibility is construed as protein and can forms the character of emulsion.
The protein solution 30ml that gets 1% concentration, adds 10ml soybean salad oil, and 10000r/min stirs 1min; Get 0.1ml emulsion from bottom with syringe, rapidly with 5ml0.1%(w/v) lauryl sodium sulfate (SDS) solution mixes, and colorimetric under 500nm wavelength, records absorbance A 0.
The emulsifying capacity of protein represents with following formula:
EA=A
0
In formula: EA---emulsibility;
A
0---the absorbance after emulsion dilution.
(4) foaming characteristic
According to the present invention, foaming characteristic is construed as the character that helps the formation that disperses gas phase.
50mmol/L potassium dihydrogen phosphate-NaOH (pH7.0) buffer solution for rice protein sample is made into 0.3% concentration solution, gets 100ml and pour in high-speed tissue mashing machine, 10000r/min stirs 1min; Proceed to 250ml graduated cylinder, record as early as possible foam volume V
0.
Protein foamability calculates according to following formula:
FC=V
0
In formula: FC---foaming characteristic;
V
0---foam volume.
The present invention has following useful technique effect:
Whole process using physical method of the present invention (dispersion and alkali treatment, freezing-pulverizing, acid neutralize, centrifugal, spraying is dry etc.) rice protein is carried out to modification, first, dispersion and alkali treatment can suitably reduce the aggregation extent of rice protein, increase the action site of protein molecule and hydrone; Under alkali condition (pH10.5～12.5) freezing-pulverization process can change aggregation extent and the protein conformation of rice protein, protein aggregation degree reduces, protein conformation launches, and the hydrophilic radical of albumen is exposed, and reaches a kind of effect of dissolving rice protein.Can prepare rice protein product (protein solubility >=98% with high dissolubility by various processes synergy of the present invention, emulsibility >=0.50, foaming characteristic >=17.39), compared with Raw Materials Rice albumen, rice protein solubility, emulsibility and foaming characteristic best result can not improve 79 times, 132% and 112%, have greatly improved utilization rate and the added value of rice protein; Especially, be 12.5 o'clock in alkali treatment pH value, protein recovery can reach 50.43%.The present invention has avoided introducing in chemical modification or enzyme modification process foreign gene or has changed protein composition, modified effect is remarkable, made high-quality, high dissolubility rice protein can be applicable to food, medicine, cosmetics and high-end health products trade, are with a wide range of applications.
Accompanying drawing explanation
Fig. 1 is process chart of the present invention.
The specific embodiment
Below in conjunction with accompanying drawing, and by embodiment, the present invention is specifically described.
Preparation Example
Embodiment 1
The present embodiment method step is as follows:
(1) disperse and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 15L water, stirs and evenly mixs, gained rice protein dispersion liquid pH value is adjusted to 10.5 with NaOH solution;
(2) freezing: step (1) gained feed liquid to be carried out to freezing processing in-10 ℃, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 10min of 1000g, is collected to supernatant;
(6) repeat: step (5) gained sediment is pressed to step (1)～(5) reprocessing 2 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, the inlet temperature of controlling spray dryer in spray-drying process is 110 ℃, and outlet temperature is 80 ℃, obtains high dissolubility rice protein finished product 53.7g.
Embodiment 2
The present embodiment method step is as follows:
(1) disperse and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 20L water, stirs and evenly mixs, gained rice protein dispersion liquid pH value is adjusted to 11.5 with NaOH solution;
(2) freezing: step (1) gained feed liquid to be carried out to freezing processing in-15 ℃, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 15min of 1000g, is collected to supernatant;
(6) repeat: step (5) gained sediment is pressed to step (1)～(5) reprocessing 2 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, the inlet temperature of controlling spray dryer in spray-drying process is 125 ℃, and outlet temperature is 90 ℃, obtains high dissolubility rice protein finished product 140.6g.
Embodiment 3
The present embodiment method step is as follows:
(1) disperse and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 20L water, stirs and evenly mixs, gained rice protein dispersion liquid pH value is adjusted to 11.5 with NaOH solution;
(2) freezing: step (1) gained feed liquid to be carried out to freezing processing in-15 ℃, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 15min of 1000g, is collected to supernatant;
(6) repeat: step (5) gained sediment is pressed to step (1)～(5) reprocessing 2 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, the inlet temperature of controlling spray dryer in spray-drying process is 125 ℃, and outlet temperature is 90 ℃, obtains high dissolubility rice protein finished product 140.6g.
Embodiment 3
The present embodiment method step is as follows:
(1) disperse and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 25L water, stirs and evenly mixs, gained rice protein dispersion liquid pH value is adjusted to 12.5 with NaOH solution;
(2) freezing: step (1) gained feed liquid to be carried out to freezing processing in-20 ℃, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 20min of 1000g, is collected to supernatant;
(6) repeat: step (5) gained sediment is pressed to step (1)～(5) reprocessing 2 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, the inlet temperature of controlling spray dryer in spray-drying process is 140 ℃, and outlet temperature is 100 ℃, obtains high dissolubility rice protein finished product 504.3g.
Rice protein end properties is measured
Dissolubility to the made rice protein finished product of embodiment 1～embodiment 3, emulsibility and emulsibility are measured and are analyzed as stated above, and result is as shown in table 1.
Table 1 high dissolubility rice protein performance measurement result
Can be obtained by table 1 test data analysis, the rice protein product preparing by physical modified method described in embodiment 1～embodiment 3 has high dissolubility, protein solubility >=98%, emulsibility >=0.50, foaming characteristic >=17.39, compared with Raw Materials Rice albumen, rice protein solubility, emulsibility and foaming characteristic best result can not improve 79 times, 132% and 112%, especially, its protein recovery of rice protein product of preparing by method described in embodiment 3 reaches 50.43%, is optimum embodiment.
The related raw material of above embodiment and Application Example and reagent are commercially available prod, and institute's use industrial equipment is this area conventional equipment.
The above is only the preferred embodiment of the present invention, the invention is not restricted to above embodiment.Be appreciated that the oher improvements and changes that those skilled in the art directly derive or associate without departing from the spirit and concept in the present invention, within all should thinking and being included in protection scope of the present invention.
Claims (4)
1. a physical modification preparation method for high dissolubility rice protein, is characterized in that: Raw Materials Rice albumen is scattered in water, and through alkali lye processing, freezing, pulverize, after acid neutralization, centrifuging and taking supernatant, spraying is dry, obtains high dissolubility rice protein finished product.
2. the physical modification preparation method of high dissolubility rice protein as claimed in claim 1, is characterized in that specifically comprising the following steps:
(1) disperse and alkali treatment: rice protein is scattered in water, and solid-liquid ratio 1:15～25, stir and evenly mix, and above-mentioned rice protein dispersion liquid pH value are adjusted to 10.5～12.5 with NaOH solution;
(2) freezing: step (1) gained feed liquid is carried out in-20～-10 ℃ freezing, until feed liquid fully charge;
(3) pulverize: the freezing feed liquid of step (2) gained solid is pulverized with pulverizer, until this solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0～7.5 with HCl solution;
(5) centrifugal: step (4) gained feed liquid, in the centrifugal 10～20min of 1000g, is collected to supernatant;
(6) repeat: (5) gained sediment is pressed to step (1)～(5) reprocessing 2～3 times;
(7) dry: dry by spraying after step (5) and the merging of step (6) gained supernatant, obtain high dissolubility rice protein finished product.
3. the physical modification preparation method of high dissolubility rice protein as claimed in claim 2, is characterized in that: in the described alkali treatment of step (1), described rice protein dispersion liquid pH value is adjusted to 12.5.
4. the physical modification preparation method of high dissolubility rice protein as claimed in claim 2, is characterized in that: 110～140 ℃ of the described spray drying control system spray dryer of step (7) inlet temperatures, 80～100 ℃ of outlet temperatures.
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|---|---|---|---|---|
| CN105580976A (en) * | 2016-01-06 | 2016-05-18 | 江南大学 | Method for improving rice protein solubility through combined use of alkaline method and electron beam irradiation |
| CN105660986A (en) * | 2016-01-19 | 2016-06-15 | 福建农林大学 | Method for improving dissolvability of rice protein isolates by supersonic wave-assisted alkali treatment |
| CN105694467A (en) * | 2016-03-25 | 2016-06-22 | 安徽特种电缆集团有限公司 | Antiaging cable material for wind turbine generators |
| CN105713397A (en) * | 2016-03-25 | 2016-06-29 | 安徽特种电缆集团有限公司 | Weather-proof cable material for wind turbine generator |
| CN107319482A (en) * | 2017-07-31 | 2017-11-07 | 无锡甜丰食品有限公司 | A kind of glucose syrup sweetener for having diet nutritional healthcare function |
| CN107638652A (en) * | 2016-12-01 | 2018-01-30 | 中国石油化工股份有限公司 | Resist the renovation process of molten aqueous film-forming foam extinguishing agent |
| CN109757602A (en) * | 2019-01-29 | 2019-05-17 | 广州华大生物科技有限公司 | A kind of alkaline process electron beam irradiation combination raising deliquescent method of rice protein |
| CN109892471A (en) * | 2017-12-08 | 2019-06-18 | 中粮集团有限公司 | A kind of rice gluten processing method and product |
| CN110200124A (en) * | 2019-07-02 | 2019-09-06 | 江南大学 | A kind of preparation method of high-dissolvability rice protein |
| CN110810617A (en) * | 2019-11-06 | 2020-02-21 | 江西省科学院应用化学研究所 | Preparation method of rice gluten with high emulsifying activity and high emulsifying stability |
| CN107568410B (en) * | 2017-10-12 | 2020-12-01 | 江南大学 | Method for preparing rice protein with high-solubility common-frame structure |
| CN112790270A (en) * | 2020-12-31 | 2021-05-14 | 江西金农米业集团有限公司 | Preparation method of soluble rice protein |
| CN113508863A (en) * | 2021-07-14 | 2021-10-19 | 江南大学 | Full-soluble edible fungus protein and preparation method thereof |
| CN114933630A (en) * | 2022-05-20 | 2022-08-23 | 华中农业大学 | Rice protein modification method and application |
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| CN105580976B (en) * | 2016-01-06 | 2019-07-30 | 江南大学 | A kind of alkaline process electron beam irradiation combination raising deliquescent method of rice protein |
| CN105580976A (en) * | 2016-01-06 | 2016-05-18 | 江南大学 | Method for improving rice protein solubility through combined use of alkaline method and electron beam irradiation |
| CN105660986A (en) * | 2016-01-19 | 2016-06-15 | 福建农林大学 | Method for improving dissolvability of rice protein isolates by supersonic wave-assisted alkali treatment |
| CN105694467A (en) * | 2016-03-25 | 2016-06-22 | 安徽特种电缆集团有限公司 | Antiaging cable material for wind turbine generators |
| CN105713397A (en) * | 2016-03-25 | 2016-06-29 | 安徽特种电缆集团有限公司 | Weather-proof cable material for wind turbine generator |
| CN107638652A (en) * | 2016-12-01 | 2018-01-30 | 中国石油化工股份有限公司 | Resist the renovation process of molten aqueous film-forming foam extinguishing agent |
| CN107319482A (en) * | 2017-07-31 | 2017-11-07 | 无锡甜丰食品有限公司 | A kind of glucose syrup sweetener for having diet nutritional healthcare function |
| CN107568410B (en) * | 2017-10-12 | 2020-12-01 | 江南大学 | Method for preparing rice protein with high-solubility common-frame structure |
| CN109892471A (en) * | 2017-12-08 | 2019-06-18 | 中粮集团有限公司 | A kind of rice gluten processing method and product |
| CN109892471B (en) * | 2017-12-08 | 2022-04-15 | 中粮集团有限公司 | Rice protein processing method and product |
| CN109757602A (en) * | 2019-01-29 | 2019-05-17 | 广州华大生物科技有限公司 | A kind of alkaline process electron beam irradiation combination raising deliquescent method of rice protein |
| CN110200124A (en) * | 2019-07-02 | 2019-09-06 | 江南大学 | A kind of preparation method of high-dissolvability rice protein |
| CN110200124B (en) * | 2019-07-02 | 2021-01-29 | 江南大学 | Preparation method of high-solubility rice protein |
| CN110810617A (en) * | 2019-11-06 | 2020-02-21 | 江西省科学院应用化学研究所 | Preparation method of rice gluten with high emulsifying activity and high emulsifying stability |
| CN112790270A (en) * | 2020-12-31 | 2021-05-14 | 江西金农米业集团有限公司 | Preparation method of soluble rice protein |
| CN112790270B (en) * | 2020-12-31 | 2023-04-11 | 江西金农米业集团有限公司 | Preparation method of soluble rice protein |
| CN113508863A (en) * | 2021-07-14 | 2021-10-19 | 江南大学 | Full-soluble edible fungus protein and preparation method thereof |
| CN114933630A (en) * | 2022-05-20 | 2022-08-23 | 华中农业大学 | Rice protein modification method and application |
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