CN103858873A - Anomala corpulenta motschulsky luring agent composition as well as application and luring core thereof - Google Patents
Anomala corpulenta motschulsky luring agent composition as well as application and luring core thereof Download PDFInfo
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Abstract
The invention discloses an anomala corpulenta motschulsky luring agent composition as well as an application and a luring core thereof. The anomala corpulenta motschulsky luring agent composition comprises an anomala corpulenta motschulsky sex pheromone and a synergist, wherein the weight ratio of the anomala corpulenta motschulsky sex pheromone to the synergist is 1:(0.5-3), wherein the anomala corpulenta motschulsky sex pheromone comprises a main component-5-myristyl dihydro-2(3H)-furanone, and the synergist is selected from at least one of geraniol, eugenol and phenylethyl propionate. The anomala corpulenta motschulsky luring agent composition provided by the invention can be accurately applied to anomala corpulenta motschulsky insect pest situation observation and prediction, massive trapping, and mating disruption, and has important significance on comprehensively treating anomala corpulenta motschulsky.
Description
Technical field
The present invention relates to insect sex pheromone preparing technical field, particularly relate to anomala corpulenta lure composition and application thereof and lure core.
Background technology
Anomala corpulenta [Anomala corpulenta Motschulsky (Coleoptera:Scarabaeoidea)] is distributed in the areas such as China northeast, North China and Central China more, one of the important soil insect in northern China agriculture district, harm multiple kinds of crops.The economic crops such as larva harm peanut, soybean, fruit tree are the most serious, and the blade of the trees such as imago feeding elm, willow, brings about great losses to China's agricultural production.Anomala corpulenta larval phase, is very long, and lives in undergroundly, adopts the control of the general chemistry such as high toxicant soil treatment, seed dressing to be difficult to the biopesticide method of preventing and treating the control efficiency that reaches desirable.
Compared with chemical pesticide, that application insect sex pheromone pest control has is highly sensitive, selectivity is strong, nontoxic, free from environmental pollution, not killed natural enemies and the advantage such as be difficult for developing immunity to drugs.Both at home and abroad the application study of insect infochemical is more and more paid attention to, the infochemical of more insects is identified, synthetic and field application constantly.According to female insects release property pheromones, male worm of the same race is had to very strong attracting ability principle, specifying female anomala corpulenta sex pheromone component and ratio can accurately be applied to detecting and reporting pest information, insect quarantine, disturb mating, mass trapping, reach the ideal effect of comprehensive regulation anomala corpulenta, avoid environmental pollution.The A. Corpulenta gyplure that the main component using is at present 5-myristyl dihydro-2 (3H)-furanone, cost is not high, and trap effect is pretty good, but because continuation is low, has caused higher replacement cost, is difficult to popularize.
Summary of the invention
In view of this, the object of the invention is to propose a kind of anomala corpulenta lure composition and application thereof and lure core, to improve the effect of comprehensive regulation anomala corpulenta, reducing cost accounting.
Based on above-mentioned purpose, anomala corpulenta lure composition provided by the invention comprises anomala corpulenta sex pheromone and synergist, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:0.5～3, wherein, the main component of described anomala corpulenta sex pheromone is 5-myristyl dihydro-2 (3H)-furanone, and described synergist is selected from least one in geraniol, eugenol and phenylethyl propionate.
Preferably, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:1～2.
Alternatively, described synergist comprises geraniol, eugenol and phenylethyl propionate.
Preferably, described synergist is made up of according to weight portion separately following component: 1～9 part of 1～9 part of geraniol, 1～9 part of eugenol and phenylethyl propionate.
Preferably, described synergist is made up of according to weight portion separately following component: 1～4 part of 1～3 part of geraniol, 3～7 parts of eugenols and phenylethyl propionate.
Preferably, described synergist is made up of according to weight portion separately following component: 2 parts of 2 parts of geraniols, 5 parts of eugenols and phenylethyl propionates.
Alternatively, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-1～9 part of furanone, 2,2,1～9 part of 1～9.8 part of 7-trimethyl decane and 2-propyl enanthol.
Preferably, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-1～5 part of furanone, 2,2,1～5 part of 3～8 parts of 7-trimethyl decane and 2-propyl enanthol.
Preferably, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-2 parts of furanones, 2,2,3 parts of 5 parts of 7-trimethyl decane and 2-propyl enanthols.
The present invention also provides a kind of application of anomala corpulenta lure composition, and described application comprises described synergist is applied to and contains the luring on core of anomala corpulenta sex pheromone; Or described application comprises and described synergist is mixed to after-applied luring on core to blank with anomala corpulenta sex pheromone; Or described application comprises described synergist is applied to blank luring on core, then lure core and anomala corpulenta sex pheromone collocation use by what contain described synergist.
It is a kind of for traping the core that lures of anomala corpulenta that the present invention also provides, described in lure core to contain above-mentioned anomala corpulenta lure composition.
It is above-mentioned for traping the preparation method who lures core of anomala corpulenta that the present invention also provides, comprise: the hexane solution that contains described synergist is added drop-wise to and contains the luring on core of anomala corpulenta sex pheromone, after n-hexane volatilization, carrene is added drop-wise to this and lures on core, after carrene volatilization, make the core that lures that contains anomala corpulenta sex pheromone and synergist thereof.
The present invention also provides a kind of method of control and/or monitoring anomala corpulenta, comprises the habitat that above-mentioned anomala corpulenta lure composition is put on to anomala corpulenta.
Alternatively, the consumption of described anomala corpulenta lure composition is 0.1～10 milli gram/m.
Preferably, the consumption of described anomala corpulenta lure composition is 0.5～2.5 milli gram/m.
As can be seen from above, the invention has the advantages that: 1) occupation mode is flexible, the invention provides anomala corpulenta lure composition, comprise anomala corpulenta sex pheromone and synergist thereof, can make synergist lures core and anomala corpulenta sex pheromone to lure core to be used in conjunction with, also anomala corpulenta sex pheromone solution can be mixed with its synergist solution, make lure composition and lure core to use; Multiple components can freely be selected independent use, or jointly uses according to proportioning, and independence is stronger; 2) with only containing anomala corpulenta sex pheromone containing the luring compared with core of its synergist, use the core that lures that contains anomala corpulenta sex pheromone and synergist composition thereof to show stronger trapping activity in the anomala corpulenta trapping test in field; 3) synergist composition provided by the invention is cheap, can reduce and make the cost that lures core, suitable penetration and promotion.
Embodiment
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, the present invention is described in more detail.
Along with the reinforcement of people's environmental consciousness and the requirement of Agricul tural Sustain able Development, make the Study of Prevention Technology of anomala corpulenta enter a new period in recent years.Adopt sex pheromone mass trapping to prevent and treat male anomala corpulenta, directly reduce on the one hand the harm of adult to crop, greatly reduce on the other hand its mating rate, reduce offspring's population quantity, thereby can reach the target of effective control anomala corpulenta.
The invention provides a kind of anomala corpulenta lure composition, comprise anomala corpulenta sex pheromone and synergist, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:0.5～3, wherein, the main component of described anomala corpulenta sex pheromone is 5-myristyl dihydro-2 (3H)-furanone, and described synergist is selected from least one in geraniol, eugenol and phenylethyl propionate.Preferably, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:1～2.
It should be noted that, described anomala corpulenta sex pheromone can be the anomala corpulenta sex pheromone of natural extraction, can be also the anomala corpulenta sex pheromone of artificial preparation.Alternatively, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-1～9 part of furanone, 2,2,1～9 part of 1～9.8 part of 7-trimethyl decane and 2-propyl enanthol.Preferably, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-1～5 part of furanone, 2,2,1～5 part of 3～8 parts of 7-trimethyl decane and 2-propyl enanthol.Preferably, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-2 parts of furanones, 2,2,3 parts of 5 parts of 7-trimethyl decane and 2-propyl enanthols.
Described geraniol, eugenol and phenylethyl propionate can use as the synergist of A. Corpulenta sex pheromone separately, and the synergist as A. Corpulenta sex pheromone after also can combining according to a certain percentage uses.Alternatively, described synergist is made up of according to weight portion separately following component: 1～9 part of 1～9 part of geraniol, 1～9 part of eugenol and phenylethyl propionate.Preferably, described synergist is made up of according to weight portion separately following component: 1～4 part of 1～3 part of geraniol, 3～7 parts of eugenols and phenylethyl propionate.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 4.5 parts of anomala corpulenta sex pheromones, 2 parts of geraniols, 5 parts of eugenols and 2 parts of phenylethyl propionates.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 5 parts of anomala corpulenta sex pheromones, 3 parts of geraniols and 5 parts of eugenols.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 5 parts of anomala corpulenta sex pheromones, 5 parts of eugenols and 4 parts of phenylethyl propionates.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 2.5 parts of anomala corpulenta sex pheromones and 7 parts of eugenols.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 7 parts of anomala corpulenta sex pheromones, 3 parts of geraniols, 6 parts of eugenols and 2 parts of phenylethyl propionates.
As one embodiment of the present of invention, described anomala corpulenta lure composition is made up of according to weight portion separately following component: 4.8 parts of anomala corpulenta sex pheromones, 1 part of geraniol, 4.5 parts of eugenols and 2.8 parts of phenylethyl propionates.
The present invention also provides the application of a kind of anomala corpulenta lure composition in control and/or monitoring A. Corpulenta, particularly, the application form of A. Corpulenta lure provided by the invention can have multiple, as long as can make A. Corpulenta sex pheromone and its synergist produce synergy, thereby consumption, the raising of minimizing A. Corpulenta sex pheromone are traped active.
In one embodiment, described application can comprise described synergist is applied to and contains the luring on core of anomala corpulenta sex pheromone.
In another embodiment, described application can comprise described synergist is mixed with anomala corpulenta sex pheromone after-applied to not luring on core containing the blank of anomala corpulenta sex pheromone, make synergist and lure core, then lure core and anomala corpulenta sex pheromone to lure core to be used in conjunction with this synergist.
In another embodiment, described application can comprise described synergist to mix with sex pheromone and after-appliedly lures on core to blank.
The present invention also provides the core that lures of the lure composition that contains anomala corpulenta sex pheromone and synergist thereof.
Described in also providing, the present invention lures the preparation method of core, comprise: the hexane solution that contains described synergist is added drop-wise to and contains the luring on core of anomala corpulenta sex pheromone, after n-hexane volatilization, carrene is added drop-wise to this and lures on core, after carrene volatilization, make the core that lures that contains anomala corpulenta sex pheromone and synergist thereof.Can lure core to pack in aluminium foil to seal by what make, at-20 DEG C, preserve.
Alternatively, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:0.5～3.Preferably, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:1～2.
Alternatively, the concentration of the hexane solution of described synergist can be 1～1500 ug/ml.Preferably, the concentration of the hexane solution of described synergist can be 100～900 ug/ml.
In another embodiment, synergist solution can be added drop-wise to the not blank containing anomala corpulenta sex pheromone and lure on core, make synergist and lure core.Described synergist lures the preparation method of core to comprise: the hexane solution that contains described synergist is added drop-wise to (for example,, on the partition of anti-mouthful of formula rubber stopper) on carrier; After n-hexane volatilization, carrene is added drop-wise on this carrier, after carrene volatilization, makes the core that lures that contains synergist.Can lure core to pack in aluminium foil the synergist of making and seal, at-20 DEG C, preserve, then lure core to be used in conjunction with anomala corpulenta sex pheromone.
Alternatively, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:0.5～3.Preferably, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:1～2.
Alternatively, the concentration of the hexane solution of described synergist can be 1～1500 ug/ml.Preferably, the concentration of the hexane solution of described synergist can be 100～900 ug/ml.
In another embodiment, the solution of synergist can be mixed with the solution of anomala corpulenta sex pheromone, preparation contains the core that lures of anomala corpulenta sex pheromone and synergist thereof simultaneously.The described preparation method who lures core can comprise: the hexane solution of described synergist is mixed with the hexane solution of anomala corpulenta sex pheromone, and the weight ratio that makes anomala corpulenta sex pheromone and synergist is 1:0.5～3.Mixed solution is added drop-wise on carrier (for example, anti-mouthful of formula rubber stopper partition on), after n-hexane volatilization, drip carrene, the core that lures that contains anomala corpulenta sex pheromone and synergist thereof described in making after carrene volatilization.Can lure core to pack in aluminium foil the lure composition of making and seal, at-20 DEG C, preserve.Preferably, the weight ratio of anomala corpulenta sex pheromone and synergist is 1:1～2.
Alternatively, the concentration of the hexane solution of described synergist can be 1～1500 ug/ml.Preferably, the concentration of the hexane solution of described synergist can be 100～900 ug/ml.
Alternatively, the concentration of the hexane solution of described anomala corpulenta sex pheromone can be 100～1000 ug/ml.Preferably, the concentration of the hexane solution of described anomala corpulenta sex pheromone can be 400～800 ug/ml.
The present invention also provides a kind of method of control and/or monitoring anomala corpulenta, comprises the habitat that anomala corpulenta lure composition of the present invention is put on to anomala corpulenta.Alternatively, the consumption of described anomala corpulenta lure composition is 0.1～10 milli gram/m.Preferably, the consumption of described anomala corpulenta lure composition is 0.5～2.5 milli gram/m.
In order to further illustrate the feasibility of anomala corpulenta lure composition provided by the invention, describe below in conjunction with concrete test.
Carrying out synergist on July 5th, 2013 to August 10 in Zhao Zhai village, Gao Long town, Luoyang City Yanshi City coordinates anomala corpulenta sex pheromone to lure the trap effect test of core.
The purity of component geraniol, eugenol and the phenylethyl propionate of synergist all can be purchased from ACROS or Aladdin company at the above compound of 99%().
Geraniol, eugenol and phenylethyl propionate are dissolved in n-hexane separately with the concentration of 100～900 ug/ml respectively, getting 1 milliliter of gradation drips on the partition of the rubber stopper cleaning with n-hexane, after n-hexane volatilization, 1 milliliter of carrene gradation is dripped, after carrene volatilization, make synergist and lure core.Lure core to pack in aluminium foil the synergist of single, two components of making and three components and seal, at-20 DEG C, preserve.
Described anomala corpulenta sex pheromone lures the preparation method of core as follows:
Step 1, by following three kinds of compounds:
5-myristyl dihydro-2 (3H)-furanone, 2,2,7-trimethyl decane and 2-propyl enanthol fully mix (above compound can be purchased from ACROS or Aladdin company) according to constant weight ratio, common part by weight is 1～9:1～9.8:1～9, most preferably be 2:5:3, use in the present embodiment this most preferred part by weight.
Step 2, the mixture that step 1 is obtained are dissolved in n-hexane according to a certain percentage, and the n-hexane pheromones solution concentration of preparation is about 100～1000 ug/ml conventionally, and what in the present embodiment, use is 600 ug/ml.
Step 3, pheromones solution (the approximately 2 milliliters) gradation that step 2 is obtained are added drop-wise to blank rubber and lure on core, and it is 1200 micrograms/lure cores that the anomala corpulenta sex pheromone obtaining lures the dosage of the pheromones containing on core.In experiment, to lure anomala corpulenta described in core be that sex pheromone lures approximately 1 milliliter of gradation of solution that core obtains step 2 to be added drop-wise to blank rubber to lure on core in combination, and it is 600 micrograms/lure cores that the anomala corpulenta sex pheromone obtaining lures the dosage of the pheromones containing on core.
Trap test during in field, lure core to be fixed on Scarab trapper epimere (Scarab trapper is purchased from Pherobio Technology Co., Ltd.) synergist with thin wire, trapper is suspended on to peanut varieties, growing way, rich water quality management term harmonization, the area Peanut Fields in flakes more than 10 mu, coordinates anomala corpulenta sex pheromone to lure core jointly to use simultaneously.
The object of reference of this series comparative trial is that anomala corpulenta sex pheromone lures core.Trapper is arranged to three repetitions, randomized arrangement.After arranging, from second day, the anomala corpulenta quantity that every morning, record was traped was also removed it in time, continues investigation 30 days.Result of the test is in table 2.
Table 1
Synergist described in table 1 is as shown in table 2 below.
Table 2
| Composition | Molecular formula | Molecular weight | There is plant in natural product |
| Geraniol | C 10H 18O | 154 | Rose etc. |
| Eugenol | C 10H 12O 2 | 164 | Plant clove of myrtaceae etc. |
| Phenylethyl propionate | C 11H 14O 2 | 178 | Peanut etc. |
In the test data of table 1, control group (01) is not for to use synergist to lure the effect of core; 02 to 04 group is that collocation A. Corpulenta sex pheromone lures core to use a kind of composition synergist to lure the effect of core; 05 to 28 group is that collocation A. Corpulenta sex pheromone lures core to use two kinds of composition synergists to lure the effect of core; 29 to 56 groups is that collocation A. Corpulenta sex pheromone lures core to use three kinds of composition synergists to lure the effect of core.As can be seen from the table, used contain A. Corpulenta sex pheromone synergist of the present invention lure core after, sex pheromone lures the trapping efficiency of core obviously to strengthen, and the consumption of sex pheromone greatly reduces.
As can be seen here, the invention has the advantages that: 1) occupation mode is flexible, anomala corpulenta lure composition provided by the invention, comprise anomala corpulenta sex pheromone and synergist thereof, can make synergist lures core and anomala corpulenta sex pheromone to lure core to be used in conjunction with, also anomala corpulenta sex pheromone solution can be mixed with its synergist solution, make lure composition and lure core to use; Multiple components can freely be selected independent use, or jointly uses according to proportioning, and independence is stronger; 2) with only containing anomala corpulenta sex pheromone containing the luring compared with core of its synergist, use the core that lures that contains anomala corpulenta sex pheromone and synergist composition thereof to show stronger trapping activity in the anomala corpulenta trapping test in field; 3) synergist composition provided by the invention is cheap, can reduce and make the cost that lures core, suitable penetration and promotion.
Anomala corpulenta lure composition provided by the invention can accurately be applied to anomala corpulenta detecting and reporting pest information, mass trapping, interference mating, significant for comprehensive regulation anomala corpulenta.And this anomala corpulenta lure composition preparation is simple, uses simply, condition is feasible, has very strong trapping capability, good stability; Meanwhile, this anomala corpulenta lure composition selectivity is strong, does not produce resistance, and natural enemy is not injured, nontoxic to people and animals, environmental friendliness; Strong stress resistance, is not afraid of wind, rainforest and Exposure to Sunlight; Lasting period is long, and control efficiency can maintain more than 30 days.
Those of ordinary skill in the field are to be understood that: the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (10)
1. an anomala corpulenta lure composition, it is characterized in that, comprise anomala corpulenta sex pheromone and synergist, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:0.5～3, wherein, the main component of described anomala corpulenta sex pheromone is 5-myristyl dihydro-2 (3H)-furanone, and described synergist is selected from least one in geraniol, eugenol and phenylethyl propionate.
2. anomala corpulenta lure composition according to claim 1, is characterized in that, the weight ratio of described anomala corpulenta sex pheromone and synergist is 1:1～2.
3. anomala corpulenta lure composition according to claim 1, it is characterized in that, described anomala corpulenta sex pheromone is made up of according to weight portion separately following component: 5-myristyl dihydro-2 (3H)-1～5 part of furanone, 2,1～5 part of 3～8 parts of 2,7-trimethyl decane and 2-propyl enanthol.
4. anomala corpulenta lure composition according to claim 1, is characterized in that, described synergist is made up of according to weight portion separately following component: 1～9 part of 1～9 part of geraniol, 1～9 part of eugenol and phenylethyl propionate.
5. anomala corpulenta lure composition according to claim 4, is characterized in that, described synergist is made up of according to weight portion separately following component: 1～4 part of 1～3 part of geraniol, 3～7 parts of eugenols and phenylethyl propionate.
6. according to an application for the anomala corpulenta lure composition described in any one in claim 1～5, it is characterized in that, described application comprises described synergist is applied to and contains the luring on core of anomala corpulenta sex pheromone; Or described application comprises and described synergist is mixed to after-applied luring on core to blank with anomala corpulenta sex pheromone; Or described application comprises described synergist is applied to blank luring on core, then lure core and anomala corpulenta sex pheromone collocation use by what contain described synergist.
7. for traping the core that lures of anomala corpulenta, it is characterized in that, contain the anomala corpulenta lure composition described in any one in claim 1～5.
8. a method for control and/or monitoring anomala corpulenta, is characterized in that, comprises the habitat that the anomala corpulenta lure composition described in any one in claim 1～5 is put on to anomala corpulenta.
9. the method for preventing and treating according to claim 8 and/or monitoring anomala corpulenta, is characterized in that, the consumption of described anomala corpulenta lure composition is 0.1～10 milli gram/m.
10. the method for preventing and treating according to claim 9 and/or monitoring anomala corpulenta, is characterized in that, the consumption of described anomala corpulenta lure composition is 0.5～2.5 milli gram/m.
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| CN105613490A (en) * | 2015-12-01 | 2016-06-01 | 深圳百乐宝生物农业科技有限公司 | Attractant for grub imagoes |
| CN111066791A (en) * | 2019-12-19 | 2020-04-28 | 浙江农林大学 | Chafer attractant, use method thereof and trap |
| CN111066791B (en) * | 2019-12-19 | 2021-09-14 | 浙江农林大学 | Chafer attractant, use method thereof and trap |
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