CN103849562B - Sleeve for biological sample in-vitro purification treatment - Google Patents
Sleeve for biological sample in-vitro purification treatment Download PDFInfo
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- CN103849562B CN103849562B CN201210518450.0A CN201210518450A CN103849562B CN 103849562 B CN103849562 B CN 103849562B CN 201210518450 A CN201210518450 A CN 201210518450A CN 103849562 B CN103849562 B CN 103849562B
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- shifting board
- sleeve pipe
- ring
- opening
- sealing
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- 238000000746 purification Methods 0.000 title claims abstract description 23
- 239000012472 biological sample Substances 0.000 title abstract 3
- 238000000338 in vitro Methods 0.000 title abstract 3
- 238000007789 sealing Methods 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 46
- 210000004369 blood Anatomy 0.000 claims description 32
- 239000008280 blood Substances 0.000 claims description 32
- 230000008569 process Effects 0.000 claims description 29
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- 238000005859 coupling reaction Methods 0.000 claims description 26
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- 229920001971 elastomer Polymers 0.000 claims description 8
- 238000011068 loading method Methods 0.000 claims description 4
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- 238000006049 ring expansion reaction Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
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- 210000004027 cell Anatomy 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
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- 210000004698 lymphocyte Anatomy 0.000 description 8
- 230000007547 defect Effects 0.000 description 7
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- 210000001772 blood platelet Anatomy 0.000 description 2
- FLJPGEWQYJVDPF-UHFFFAOYSA-L caesium sulfate Chemical compound [Cs+].[Cs+].[O-]S([O-])(=O)=O FLJPGEWQYJVDPF-UHFFFAOYSA-L 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
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- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
Abstract
The invention relates to a sleeve for biological sample in-vitro purification treatment. The sleeve comprises a pipe body, multiple movable baffles, a connecting shaft, a sealing ring and a coaxial locking switch, wherein the pipe body is cylindrical, a flange is arranged at the upper end of the pipe body, multiple first openings are formed in the end face of the bottom of the pipe body, and multiple second openings and convex blocks are arranged at the bottom end of the side wall of the pipe body; the sealing ring is sleeved on the outer side of the second openings; the movable baffles are arranged at the bottom of the pipe body, are of an annular fan shape and are provided with multiple third openings, and the outer edges of the third openings are in one-to-one correspondence with the second openings and are contacted with the sealing ring; the connecting shaft is positioned at a central position of the pipe body, the top end of the connecting shaft is connected with the coaxial locking switch, a polygonal part is arranged at the bottom end of the connecting shaft, and the edges of the polygonal part are in one-to-one correspondence with the inner edges of the movable baffles; the coaxial locking switch is positioned on the upper surface of the flange of the pipe body and can rotate. The sleeve provided in the invention is a device which is convenient, practical, low in production cost, simple in production process, safe and non-toxic and is used for biological sample in-vitro treatment.
Description
Technical field
The present invention relates to a kind of sleeve pipe for the process of biological specimen purification, belong to biomedicine technical field.
Background technology
The biological particles in centrifuging ex vivo enrichment biological specimen is utilized to be the technology be most widely used in biological study.Many biological particless all can realize enrichment by this technology of centrifuging, such as, and the cell of animal or plant, subcellular structure, bacterium, virus or biomacromolecule etc.These biological particles performances under the action of the centrifugal in suspension depend primarily on size and the density of biological particles, and this can pass through Stokes equations (Stokes equation) and be explained.Stokes equations (see following formula) describes the Changing Pattern of movement rate when spheroidal particle is subject to centrifugation in liquid medium:
Wherein: d represents the diameter of spheroidal particle, p
2represent the density of spheroidal particle, p
1represent the density of liquid medium, n represents the viscosity of liquid medium, and g represents centrifugal force.Known by Stokes equations, when the equal density of particle and liquid medium, the subsidence rate of particle in centrifugal field is zero, and this is the theoretical basis utilizing centrifuging enrichment particular organisms particle.Such as, when utilizing the lymphocyte in centrifuging ex vivo enrichment human blood sample, because the density of human lymphocyte is about 1.077g/cm
3, density can be utilized to be all 1.077g/cm
3density medium realize this process of lymphocyte-rich from human blood sample; And for example, the density of DNA is at 1.500-1.800g/cm
3between, then the raw materials such as the cesium chloride that can be mixed with this density range solution or cesium sulfate should be selected as density medium formula.
Utilize centrifugal principle to carry out the process of biological specimen purification, namely from biological specimen, the step of enriched biological particle is basic simlarity.Such as, when utilizing density medium to carry out the ex vivo enrichment of human lymphocyte in blood, its general operation steps comprises: (1) carries out pre-dilution with a certain proportion of balanced salt solution or physiological saline to blood sample; (2) blood sample after dilution is added along centrifuge container wall carefully and is preinstalled with in the centrifuge container of density medium, keep the interface of sample and medium clear, do not mix, this process is the committed step ensureing concentration effect, is often called as " paving blood "; (3) carry out under certain temperature and certain centrifugal condition centrifugal, such as 20 DEG C time, centrifugal force is under the condition of 400g centrifugal 30 minutes; (4) from whizzer, carefully take out the centrifuge container that sample is housed, utilize the device such as pasteur pipet or transfer pipet to draw " the tunica albuginea layer " that be enriched in sample and medium layer interface place; (5) cellular product obtained is washed with a certain proportion of balanced salt solution, physiological saline or cell culture medium, removing thrombocyte, cell debris and part dead cell, and the density medium of remnants; (6) cell suspension obtained is used for the subsequent experimental such as cell cultures.
For realizing good concentration effect, aforesaid operations step is very high for the technical requirements of operator.Such as, in the operation of above-mentioned steps (2), the blood sample after dilution must slowly be layered on density medium by operator very carefully, keeps the interface of sample and medium clear, does not mix.This is because, if blood sample mixes with density medium, " the tunica albuginea layer " be finally enriched between sample and medium layer will become very loose, and containing a large amount of red blood cell contamination, this operates the obstacle brought in visual determination not only can to the collection in step (4), also can produce serious detrimentally affect to the yield of object cell and purity.Therefore, the feature of " paving blood " this operation be not only time-consuming, but also to require great effort, the technical requirements for operator is high, is unfavorable for realizing mass-producing process, and during especially for clinical treatment purposes, the mass-producing process of a large amount of blood sample is a large technical barrier.Again such as, in the operation of above-mentioned steps (4), first operator needs accurately to judge the position of " tunica albuginea layer ", then utilize the device such as pasteur pipet or transfer pipet to stretch into below liquid level and draw the object cell being enriched in this region, this not only wastes time and energy, and be constantly sucked out along with object cell, the thickness of tunica albuginea layer reduces gradually, outward appearance and position also more and more not obvious, when this should stop the judgement of suction operation again to propose higher requirement for operator, if there is the large mistake in judgement to the surplus of cell in operator, stopped suction operation in advance, then the yield of object cell will obviously reduce.
Based on the problem in aforesaid operations, some solutions are proposed in succession.Such as, some overseas enterprises develop and are specifically designed to the lymphocytic centrifuge tube of purifying from blood sample, the representational product comprising the exploitation of German Greinerbio-one company
centrifuge tube, the feature of this centrifuge tube is that slightly bottom installation secures one deck porous filter screen barrier in pipe, is divided by centrifuge tube in order to upper and lower two compartments.Because the aperture of barrier is smaller, blood sample at once through barrier, cannot can be poured directly into lower compartment and be preinstalled with in the centrifuge tube of density medium, and need not mix with density medium by concern over blood sample by blood sample like this.In follow-up centrifugally operated, because the radius of hemocyte is much smaller than the aperture of barrier, therefore hemocyte can freely pass through screen barrier and contact with medium layer, and does not affect layered effect.Such device solves the difficulty in " paving blood " operation described in above-mentioned steps (2) preferably, substantially reduces the operating time, improves working efficiency and effect.But, this defect with the centrifuge tube of porous filter screen barrier structure is also fairly obvious, specifically comprise following some: (a) due to the aperture of barrier smaller, not only blood sample cannot at once through barrier, density medium equally also cannot at once through barrier, therefore the standard operation being applicable to this device is that first upwards compartment adds appropriate density medium, such as Ficoll-Paque
tMplus, centrifugal then by several minutes, make density medium all enter lower compartment through barrier, can carry out follow-up " paving blood " operation, this introduces extra trouble again to the lock out operation of blood sample.In addition, the amount needs of the density medium added obtain controlling very accurately, and it a little less than the height of barrier, if add-on is very few, will should have a negative impact to purification effect; If add-on is too much, then also need with receiving the density medium exceeding barrier height, this is similarly lock out operation and brings inconvenience.B () has the production technique more complicated of the centrifuge tube of porous filter screen barrier structure, only external Ji Jia enterprise can complete process for processing at present, and domestic shortage proven technique and corresponding product.This is main because install fixing one deck porous filter screen barrier structure and propose very high requirement to adhering technique on smooth centrifugal tube wall.Bonding not only needs enough physical strengths to ensure that in transport and centrifugally operated, barrier can not come off, and the more important thing is, the selection of tackiness agent need consideration water insoluble, to Biosafety sexual factors such as cytotoxic are harmless.The problem of tackiness agent is transformed to the potential clinical application of this series products and brings huge obstruction.C (), based on the complicacy of technical matters, this product involves great expense, every brace has the centrifuge tube of porous filter screen barrier structure to be about tens doubly to one hundred times of same capacity centrifugation pipe price, which greatly limits widely using of this series products.
For addressing the aforementioned drawbacks the problem described in (a), Sigma Co., USA develops product A CCUSPIN
tMsystem-
-1077, it is a kind of centrifuge tube with porous filter screen barrier structure being preinstalled with appropriate density medium.The solution of prepackage density medium solves the problem described in above-mentioned defect (a) really very well, but brings again defect as described below: the storing mode of (d) this series products must set separately for density medium.ACCUSPIN
tMsystem-
explicitly point out in the specification sheets of-1077, this centrifuge tube with porous filter screen barrier structure being preinstalled with appropriate density medium must 2-8 DEG C, preserve under lucifuge condition, volume shared by centrifuge tube is comparatively large, and this special preserving type is that the application of this series products brings very big inconvenience.E (), under the immersion that water-based density medium is long-term, whether the adhesive effect of screen barrier and result of extraction can also meet service requirements lacks sufficient testing data support.To sum up, (b) during the centrifuge tube with porous filter screen barrier structure being preinstalled with appropriate density medium not only cannot address the aforementioned drawbacks, problem described in (c), also additionally introduce the problem of defect (d) and the preservation described in (e), Product Safety and validity.
Chinese patent 200610063125.4 provides a kind of separation of lymphocytes pipe being provided with loose porous material at the bottom of pipe, density medium absorption can be become semi-solid state by this loose porous material, when carrying out the purifying of blood sample, can directly pour blood sample into this separator tube, and the interface mixing up both blood and parting liquid need not be worried, solve " paving blood " very well and operate the problem wasted time and energy.And, owing to can realize fixing by frictional force between described loose porous material and body, thus this technology does not need to use tackiness agent, and technology difficulty reduces greatly, thus overcomes (b) and the problem described in (c) in above-mentioned defect.But, this separation of lymphocytes pipe being provided with loose porous material at the bottom of pipe still faces the time consuming procedures found of density medium adition process, due to loose porous material can stop blood at once through, absorption and the infiltration of density medium also need certain hour, need could be realized by " dropping "; Further, because loose porous material itself is not transparent, the add-on of density medium also more difficult realization accurately controls, this so that very large difficulty is brought for the control of blood sample separating effect and the repeatability of result.Above-mentioned patent additionally provides another kind of product form, namely in advance lymphocyte separation medium (density medium) is added in the loose porous material of body, although this can realize the accurate control of " plug and play " and density medium add-on, but the material of loose porous material is faced with the problem of above-mentioned defect (d) and the preservation described in (e), Product Safety and validity equally.Namely, the density medium added in advance by long period of soaking in loose porous material, loose porous material (the extraordinary sponge recorded in the such as above-mentioned patent) composition whether meeting stripping is harmful to for hemocyte under this soaking conditions, this needs to be proved further by lot of experiments.
Summary of the invention
For solving the problems of the technologies described above; the object of the present invention is to provide a kind of sleeve pipe for the process of biological specimen purification; this sleeve pipe can load among centrifuge container; for the purification process of biological specimen; this sleeve pipe have convenient and practical, production technique is simple, cost is low and the feature of safety non-toxic; and be particularly suitable for the mass-producing process of biological specimen, make operating process obtain the simplification of high degree.
The present invention also aims to provide a kind of centrifuge container comprising above-mentioned sleeve pipe.
The present invention also aims to a kind of method that biological specimen purification process utilizing above-mentioned centrifuge container to carry out is provided.
For achieving the above object, the present invention provide firstly a kind of sleeve pipe for the process of biological specimen purification, and it comprises body, some pieces of shifting boards, coupling shaft, sealing-ring, coaxial locking keyes, wherein:
Described body is tubular (being preferably cylindric), its upper end has export-oriented flange, its bottom end face is provided with several the first openings, the bottom of its sidewall is provided with the projection of several the second openings and several extroversions, wherein, described projection is positioned at the top of the connection section of adjacent two described second openings;
Described sealing-ring is placed on the outside of described second opening part;
The bottom of described body is located at by described shifting board, and described shifting board is provided with several the 3rd openings, and the outer rim of described shifting board is with described second opening one_to_one corresponding and contact with described sealing-ring;
Described coupling shaft is positioned at the central position of described body, and its top is connected with described coaxial locking key, and bottom is provided with a polygonal features, and each limit of this polygonal features is with the inner edge one_to_one corresponding of a described shifting board and contact;
The upper surface that described coaxial locking key is positioned at the flange of described body can rotate along this upper surface;
When described shifting board is in open mode, overlap at least partly between described first opening and the 3rd opening; When described shifting board is in closure state, there is not the part of coincidence between described first opening and the 3rd opening, the shape and size of described second opening and the outer rim of described shifting board match.
Above-mentioned sleeve pipe provided by the invention for loading in centrifuge container (such as common centrifuge tube, centrifugal bottle), to realize the enrichment to the biological particles in biological specimen.For ease of sleeve pipe being put into centrifuge container or being taken out, the external diameter of this casing pipe is slightly less than the internal diameter of centrifuge container, the two movable sleeve is joined, and the length of this sleeve pipe (or claiming height) can be determined in conjunction with the size of centrifuge container as required.The size of the flange of this cannula tip is fixed on centrifuge container can make casing hanger, and the use not affecting centrifuge container lid is advisable, and is preferably equal to or slightly less than the full diameter of thread of centrifuge container.The lateral dimension (external diameter or the height relative to tube wall) being arranged in the projection of casing pipe bottom should enable this projection comparatively closely contact with centrifuge container inwall, enable sleeve pipe put into centrifuge container smoothly and take out again, preferably, the diameter of circle that forms of projection outer rim is identical with the internal diameter of centrifuge container or be slightly less than its internal diameter.In addition, the bottom surface of this projection should contact with each other with sealing-ring, it is avoided to upwarp sealing-ring can be pushed down, simultaneously, when shifting board is in open mode, the outer that this projection also can push down shifting board avoids it to upwarp, and the partial gap produced after can also being opened by shifting board together with rubber ring seals up.
In above-mentioned sleeve pipe, shifting board is arranged on the bottom of body, and can along radial direction free movement within the specific limits.The outer rim of shifting board is arc, linking together to form a circle.The biological particles in biological specimen each block shifting board is all provided with the 3rd opening of some amount, so that can pass through.Shifting board has open and close two states, when shifting board is in closure state, whole shifting boards forms a closed ring-type, outer rim connects together the closed circle of formation one, and, the Polygons cavity at center or polygonal grooves are just in time filled by the polygonal features of coupling shaft bottom, and thus, the polygonal features of shifting board and coupling shaft bottom forms closed circle or disk.According to specific embodiment of the invention scheme, preferably, sleeve pipe provided by the invention comprises 3-12 (preferred 3-6) shifting board, and the limit number of the quantity of the second opening, the quantity of projection, polygonal features is identical with the quantity of shifting board.Corresponding to 3-12 shifting board, the polygonal features bottom coupling shaft can be equilateral triangle-dodecagon, and corresponding to 3-6 shifting board, the polygonal features bottom coupling shaft can be equilateral triangle-regular hexagon.
According to specific embodiment of the invention scheme, preferably, shifting board is that ring is fan-shaped, and the thickness of shifting board is identical with the thickness of polygonal features, and all shifting boards and polygonal features form a complete disc; Or shifting board is fan-shaped, the thickness of the centre portions of baffle plate is lower than the thickness of Outboard Sections, and, the thickness of the centre portions of shifting board and the thickness sum of polygonal features are equal to or greater than the thickness of shifting board Outboard Sections, and all shifting boards and polygonal features form a complete disc.When shifting board be ring fan-shaped time, its inner rim can be linear pattern, and all shifting boards form the disk that a centre has Polygons cavity, and this Polygons cavity is identical with the shape of the polygonal features of coupling shaft bottom; When shifting board is fan-shaped, its centre portions divides thinner than outer half, the centre portions of all shifting boards is combined together to form a reeded disk in centre, the limit of this groove is preferably linear pattern, its shape and polygonal features can cooperatively interact, polygonal features also slightly thinner in case with the thickness matching of shifting board, by the middle portion of shifting board is pressed in below polygonal features, shifting board can be made to be arranged on sleeve bottom preferably, can not tilt, in order to avoid affect sealing effectiveness.
According to specific embodiment of the invention scheme, preferably, the outer edge side mask of shifting board has a groove matched with sealing-ring.Sealing-ring can be enclosed within this groove, keeps stability, to avoid slippage.
The first opening that its bottom end face of sleeve pipe is offered is for making the biological particles in biological specimen (such as hemocyte) freely can pass through in centrifugal field and contact with the density medium be contained in centrifuge container.The shape of this first opening and size can be selected as required, but will adapt with the 3rd opening on shifting board, to enable biological specimen be contacted with density medium by the intersection of the first opening with the 3rd opening when open mode.According to specific embodiment of the invention scheme, preferably, when shifting board is in open mode, overlaps at least partly between the first opening and the 3rd opening and (preferably overlap completely, in such cases, the first opening of correspondence position and the 3rd opening can be of similar shape); When shifting board is in closure state, there is not the part of coincidence between the first opening and the 3rd opening, namely close mutually.
The second opening that the sidewall of sleeve pipe is arranged can be arranged as required, its quantity should be consistent with the quantity of shifting board, and, this second opening should enable shifting board freely come in and go out when open mode and meet certain closed needs, namely the size of the second opening and shape should with the physical dimension of shifting board and mating shapes.The quantity of the projection that tube wall is arranged is also identical with the quantity of shifting board.Above-mentioned second opening is arranged along the outer wall of sleeve pipe, and it can be rectangle (or claiming the rectangle of curved surface), and namely bottom and upper segment is parallel with the bottom end face of sleeve pipe respectively, and the left side and the right are vertical with the bottom end face of sleeve pipe respectively.
In sleeve pipe provided by the invention, sealing-ring is sheathed on the outside of the second opening, so that in use to the sealing gap between sleeve pipe and centrifuge container.According to specific embodiment of the invention scheme, preferably, the thickness of sealing-ring is identical with the height of the second opening.Sealing circle can be any circle that can realize sealing, and is preferably resilient sealing-ring, such as rubber ring.The internal diameter of sealing circle can be identical with the external diameter of body, so that sheathed.The inner edge of sealing circle contacts with the outer rim of shifting board, and the outer rim of each shifting board connects together formed circular diameter should be identical with the internal diameter of sealing-ring.
In sleeve pipe provided by the invention, when shifting board is in closed state, state that it is in " contraction ", state that sealing-ring is also in " contraction " (not having elastically-deformable state), its external diameter is slightly less than the internal diameter of centrifuge container, gap between casing pipe and centrifuge container can not be sealed, casing pipe can freely take out or put into from centrifuge container, the 3rd opening now on shifting board blocks by differing to each other mutually with the first opening of its bottom, body is in relatively closed state, biological specimen not by, and when shifting board is in open mode, state that it is in " expansion ", the 3rd opening on shifting board overlaps each other at least partly with the first opening of its bottom, biological specimen can freely pass through, simultaneously, sealing-ring is also along with the expansion of shifting board, outwards pushed along radial direction by the edge of shifting board, become " expansion " state and with centrifuge container outer wall close contact, and together with the projection of body bottom by the gap sealing (now the external diameter of sealing-ring reaches the size of centrifuge container internal diameter) between casing pipe and centrifuge container inwall, biological specimen not by, even if also cannot this gap be passed through in centrifugal field.
In above-mentioned sleeve pipe, the polygonal features of coupling shaft bottom and shifting board by central side close contact, it is synchronously realized by the rotation of polygonal features, coupling shaft and coaxial locking key that the state of shifting board switches (open and close).Correspondingly, polygonal features also can have open and close two kinds of using states, and (description of open and close herein defines to be consistent with the state of shifting board, and do not mean that the shape of polygonal features itself will be opened or close), when polygonal features is in closure state, the edge of polygonal features and contacting by central side sides aligned parallel of shifting board, shifting board is in closure state, and sealing-ring shrinks, and body is in relative closure state; When polygonal features is in open mode, the summit of polygonal features and shifting board by central side edge contact, shifting board outwards produces the displacement of certain distance along radial direction, the 3rd opening on shifting board overlaps each other at least partly with the first opening of its bottom, forwards open mode to by closure state.
In above-mentioned sleeve pipe, coupling shaft be positioned at body center, overlap with the central shaft of body, its top is connected with coaxial locking key.Coaxial locking key can rotate by rotarily driving coupling shaft, thus drives and change the location status of polygonal features, so realize shifting board closed with open mode between switching.When shifting board is under two kinds of location status that are closed and that open, coaxial locking key all by the realization of certain latch-up structure at the locking position of body upper end annular flange flange, can be avoided shifting board in centrifugal field or under carrying state, unexpected displacement occurs.Above-mentioned latch-up structure can be any device that can realize above-mentioned locking, preferably, the flange of sleeve pipe is provided with at least two to (four) lock slots, this lock slots is relative between two, coaxial locking key comprises a cross bar, be connected with coupling shaft top in the middle of this cross bar and coupling shaft can be driven to rotate, its two ends lay respectively among pair of locking groove and also can change between different lock slots.Such as, the flange of sleeve pipe arranges four lock slots (groove), make it relative between two, be connected with coupling shaft top in the middle of the cross bar of coaxial latch-up structure and coupling shaft can be driven to rotate, its two ends lay respectively among pair of locking groove, this cross bar can turn to down in a pair lock slots under external force, can realize shifting board conversion between opened and closed conditions by rotating.As long as the shape of cross bar is applicable to lock slots shape, the size of cross bar is suitable with the size of lock slots, makes cross bar have certain resistance when falling into lock slots, ensures that cross bar is fixed by resistance in a longitudinal direction; The two ends of this cross bar can comprise two bar heads upwards, so that apply external force rotating horizontal pole and two power to applying sensing center, cross bar two ends, cross bar two is tilted and departs from pair of locking groove, and can be slided into another in lock slots along the flange of sleeve pipe when keeping cross bar to tilt state at two; When cross bar is in lock slots, it can be locked in turning direction, can not open arbitrarily with the position and state locking, guarantee shifting board that realize shifting board; Meanwhile, because cross bar is fixed by the resistance between lock slots in a longitudinal direction, whole coupling shaft can not be up and down together with polygonal features, also achieves locking in a longitudinal direction.When pointing to a pair External Force Acting at center in cross bar two ends and when reaching a certain size, cross bar can be made temporarily to spin off from lock slots and forward another to locked in lock slots, this cross bar preferably has certain elasticity, so that by roll-in and roll-out; Distance between two pairs of lock slots can be determined according to the quantity of shifting board, as long as the conversion between the open and close state that can realize shifting board and locking.
The material that sleeve pipe provided by the present invention adopts can be any medical material meeting related sanitary standard, the material of such as this sleeve pipe can be the plastic material of the syringe for the preparation of clinical injection, the elastomeric material sealing the rubber ring adopted can be the medical grade rubber meeting hygienic standard, such as such as, for making the elastomeric material of the rubber components of the injector push-rod leading portion of clinical injection, medical grade silicon rubber etc.
Present invention also offers a kind of centrifuge container for the process of biological specimen purification, it comprises the above-mentioned sleeve pipe for the process of biological specimen purification and centrifuge container body, and wherein, sleeve pipe is positioned among centrifuge container body and the two movable sleeve is joined.
According to specific embodiment of the invention scheme, preferably, above-mentioned centrifuge container can be centrifuge tube or centrifugal bottle etc.
Present invention also offers a kind of method for the process of biological specimen purification, it adopts above-mentioned centrifuge container to carry out enrichment to the biological particles in biological specimen, and it comprises the following steps:
Sleeve pipe is assemblied in centrifuge container, and opens the shifting board of sleeve pipe;
Density medium is loaded in centrifuge container body, close the shifting board of sleeve pipe;
By in biological specimen sprue bushing;
Open the shifting board of sleeve pipe, biological specimen is contacted with density medium, carry out centrifugal treating, after centrifugal treating, above the shifting board of sleeve pipe, form biological particles enriched layer;
Close the shifting board of sleeve pipe, sleeve pipe is taken out from centrifuge container body, biological particles enriched layer is shifted, completes the enrichment of biological particles in biological specimen.
By aforesaid method be separated the biological particles enriched layer that obtains can by after suitable process for different purposes, concrete process can be carried out according to usual manner.
In the above-mentioned methods, the amount of the density medium of loading can be determined as required, and preferably, the upper surface of density medium loaded in centrifuge container body contacts with the bottom face of sleeve pipe or a little more than bottom face.
Aforesaid method provided by the present invention may be used for the enrichment of existing biological specimen, and preferably, above-mentioned biological specimen is blood or tissue samples etc., and the biological particles in biological specimen is lymphocyte, monocyte, hemopoietic stem cell, mescenchymal stem cell etc.
Sleeve pipe provided by the present invention proposes for the problem that density gradient centrifugation is existing during enriched biological particle from the biological specimen such as blood or tissue samples, and can solve " paving blood " operation wastes time and energy with " drawing tunica albuginea layer " operation and be unfavorable for the problem of mass-producing process biological specimen; The currently available products provided in background technology or the defect described in (a)-(e) existing for technology also can be provided.Sleeve pipe provided by the present invention is the simple and device for the process of biological specimen purification of safety non-toxic of a kind of convenient and practical, low production cost, production technique.
Accompanying drawing explanation
The one-piece construction schematic diagram of the sleeve pipe that Fig. 1 provides for embodiment 1;
The shifting board of the sleeve pipe that Fig. 2 provides for embodiment 1 and the exploded perspective view of coupling shaft;
The closure state schematic diagram of the shifting board of the sleeve pipe that Fig. 3 provides for embodiment 1;
The open mode schematic diagram of the shifting board of the sleeve pipe that Fig. 4 provides for embodiment 1;
First opening schematic diagram of the sleeve bottom end face that Fig. 5 provides for embodiment 1;
Second opening of the sleeve pipe side that Fig. 6 provides for embodiment 1 and projection schematic diagram;
Fig. 7 is the structural representation of shifting board in embodiment 2 and polygonal features;
Fig. 8 is the set-up mode schematic diagram of coaxial locking key in embodiment 1;
Fig. 9 is that the A-A of Fig. 8 is to sectional view;
The schematic flow sheet of the method for the process of biological specimen purification that Figure 10 provides for embodiment 4.
Main Reference label declaration:
Body 1 shifting board 2 coupling shaft 3 sealing-ring 4 coaxial locking key 5 flange 6 first opening 7 projection 8 second opening 9 the 3rd opening 10 polygonal features 11 connection section 12 cross bar 13 bar head 14 lock slots 15
Embodiment
In order to there be understanding clearly to technical characteristic of the present invention, object and beneficial effect, existing following detailed description is carried out to technical scheme of the present invention, but can not be interpreted as to of the present invention can the restriction of practical range.
Embodiment 1
Present embodiments provide a kind of sleeve pipe for the process of biological specimen purification, it comprises body 1, six pieces of shifting boards 2, coupling shaft 3, sealing-ring 4, coaxial locking key 5, as shown in Figure 1, wherein:
The upper end of body 1 has export-oriented flange 6, the bottom end face of body 1 is provided with several the first openings 7 (as shown in Figure 5), the bottom of its sidewall is provided with the projection 8 of several the second openings 9 and several extroversions, wherein, projection 8 is positioned at the top (as shown in Figure 6) of the connection section 12 of adjacent two the second openings 9;
Sealing-ring 4 is a rubber ring, is placed on the outside at the second opening 9 place;
The bottom of body 1 is located at by shifting board 2, and it is that ring is fan-shaped, which is provided with several the 3rd openings 10, and the outer rim of this shifting board 2 is with the second opening 9 one_to_one corresponding and contact (as shown in Figure 1, Figure 2, Figure 3, Figure 4) with sealing-ring 4;
Coupling shaft 3 is positioned at the central position of body 1, its top is connected with coaxial locking key 5, bottom is provided with a polygonal features 11 (being regular hexagon in the present embodiment), and each limit of this polygonal features 11 is with the inner edge one_to_one corresponding of a shifting board 2 and contact (as shown in Figure 2,3);
The upper surface that coaxial locking key 5 is positioned at the flange 6 of body 1 can rotate along this upper surface;
This coaxial locking key 5 comprises a cross bar 13 and is located at the bar head 14 at cross bar 13 two ends, as shown in Figure 8 and Figure 9, the flange 6 of body 1 upper end is provided with two to (four) lock slots 15, relative between two, the two ends of cross bar 13 are positioned at wherein locked among pair of locking groove 15, now shifting board 2 is in open mode, when applying a certain size a pair power at sensing center to bar head 14, there is certain elastic cross bar 13 depart from from this pair lock slots 15, keep this to power and apply tangential external force to bar head making cross bar 13 forward another to making cross bar 13 fall into and be locked among lock slots 15, coupling shaft 3 can be driven when cross bar 13 rotates, polygonal features 11 rotates together, shifting board 2 is finally made outwards to produce certain displacement along radial direction, it forwards closure state to by open mode.
Embodiment 2
Present embodiments provide a kind of sleeve pipe for the process of biological specimen purification, wherein, the structure of shifting board 2 and polygonal features 11 as shown in Figure 7, shifting board 2 comprises centre portions and Outboard Sections, the thickness of its centre portions is lower than Outboard Sections, when shifting board 2 is combined, a reeded disk in centre can be formed; The outer edge mask of this shifting board 2 has a groove, the size match of this groove and sealing-ring 4, can make above sealing-ring 4 is enclosed within, to prevent slippage and displacement;
And polygonal features 11 is also thinner relative to the polygonal features 11 in embodiment 1, its size just in time combines the groove match in the middle of the disk of formation with shifting board 2, other structures of this sleeve pipe are identical with embodiment 1.
Embodiment 3
Present embodiments provide a kind of centrifuge tube for the process of biological specimen purification, it comprises the sleeve pipe that a common centrifuge tube body and embodiment 1 or 2 provide, and this sleeve pipe to be positioned among centrifuge tube body and to join with its movable sleeve.
Shifting board 2 has closed and opens two states, respectively as shown in Figure 3 and Figure 4, wherein: when in a closed state, six pieces of shifting boards 2 mutually combine and form a circle with polygonal features 11, sealing-ring 4 is now also in contraction schedule, be enclosed within the outside of body 1 second opening 9 and contact with the outer rim of shifting board 2, there is not overlapping region in the first opening 7 bottom body 1 and the 3rd opening 10 on shifting board 2, body 1 is in the state of a relative closure, by rotating coaxial locking key 5, coupling shaft 3 and polygonal features 11 can be driven to rotate, when polygonal features 11 rotates, its side can promote shifting board 2 and makes it outwards move along radial direction, open mode is transformed into by closure state, in this condition, shifting board 2 by sealing-ring 4 to extrapolation, sealing-ring 4 is expanded and touches the inwall of centrifuge tube body, by the gap sealing between sleeve pipe and centrifuge tube body, the first opening 7 bottom body 1 and the 3rd opening 10 on shifting board 2 overlap, body 1 is in the state of an opening, the liquid being positioned at the upper and lower both sides of box face can be allowed to contact with each other.
Embodiment 4
Present embodiments provide a kind of method for the process of biological specimen purification, it is the centrifuge tube adopting embodiment 3 to provide carries out enrichment method to the mononuclearcell in blood sample, and its flow process as shown in Figure 10.The method comprises the following steps:
Sleeve pipe is placed in centrifuge tube body interior, opens the shifting board 2 of sleeve pipe;
Density medium loaded in centrifuge tube body, the liquid level loading density medium reaches the height contacted with casing shoe face, closes the shifting board 2 of sleeve pipe;
Blood sample is poured directly in sleeve pipe, without the need to carefully injecting along internal surface of sleeve pipe;
Open the shifting board 2 of sleeve pipe, blood sample contacted with density medium, 20 DEG C, carry out centrifugal treating under the condition of 400g centrifugal force, after centrifugal 30 minutes, above the shifting board 2 of sleeve pipe, form tunica albuginea layer, i.e. mononuclearcell layer;
Close the shifting board 2 of sleeve pipe, sleeve pipe is taken out from centrifuge tube body, the plasma layer pasteur pipet or pipette, extract that are rich in thrombocyte and cell debris are shifted out, and tunica albuginea layer is shifted, complete the enrichment of the mononuclearcell in blood sample.
Claims (12)
1., for a sleeve pipe for biological specimen purification process, it comprises body, some pieces of shifting boards, coupling shaft, sealing-ring, coaxial locking keyes, wherein:
Described body is tubular, its upper end has export-oriented flange, and its bottom end face is provided with several the first openings, and the bottom of its sidewall is provided with the projection of several the second openings and several extroversions, wherein, described projection is positioned at the top of the connection section of adjacent two described second openings;
Described sealing-ring is placed on the outside of described second opening part;
The bottom of described body is located at by described shifting board, and described shifting board is provided with several the 3rd openings, and the outer rim of described shifting board is with described second opening one_to_one corresponding and contact with described sealing-ring, and described sealing-ring is resilient sealing-ring;
Described coupling shaft is positioned at the central position of described body, and its top is connected with described coaxial locking key, and bottom is provided with a polygonal features, and each limit of this polygonal features is with the inner edge one_to_one corresponding of a described shifting board and contact;
The upper surface that described coaxial locking key is positioned at the flange of described body can rotate along this upper surface;
When described shifting board is in open mode, overlap at least partly between described first opening and the 3rd opening; When described shifting board is in closure state, there is not the part of coincidence between described first opening and the 3rd opening, the shape and size of described second opening and the outer rim of described shifting board match.
2. sleeve pipe according to claim 1, wherein, the thickness of described sealing-ring is identical with the height of described second opening.
3. sleeve pipe according to claim 1, wherein, described sealing-ring is rubber ring.
4. sleeve pipe according to claim 1, wherein, described shifting board is that ring is fan-shaped, and the thickness of described shifting board is identical with the thickness of described polygonal features, and all shifting boards and described polygonal features form a complete disc; Or described shifting board is fan-shaped, the thickness of the centre portions of described baffle plate is lower than the thickness of Outboard Sections, and, the thickness of the centre portions of described shifting board and the thickness sum of described polygonal features are equal to or greater than the thickness of described shifting board Outboard Sections, and all shifting boards and described polygonal features form a complete disc.
5. sleeve pipe according to claim 1, wherein, this sleeve pipe comprises 3-12 shifting board, and the limit number of the quantity of described second opening, the quantity of described projection, described polygonal features is identical with the quantity of described shifting board.
6. the sleeve pipe according to claim 1 or 4, wherein, the outer edge side mask of described shifting board has a groove matched with sealing-ring.
7. sleeve pipe according to claim 1, wherein, the flange of described sleeve pipe is provided with at least two pairs of lock slots, described coaxial locking key comprises a cross bar, be connected with coupling shaft top in the middle of this cross bar and coupling shaft can be driven to rotate, its two ends lay respectively among pair of locking groove and also can change between different lock slots.
8. the centrifuge container for the process of biological specimen purification, it comprises the sleeve pipe for the process of biological specimen purification described in any one of claim 1-7 and centrifuge container body, wherein, described sleeve pipe to be positioned among centrifuge container body and the two movable sleeve is joined.
9. centrifuge container according to claim 8, wherein, described centrifuge container is centrifuge tube or centrifugal bottle.
10. centrifuge container according to claim 8, wherein, when described shifting board is in open mode, described sealing-ring expansion also can by the gap sealing between described centrifuge container body and described sleeve pipe.
11. 1 kinds of methods for the process of biological specimen purification, it adopts the centrifuge container described in any one of claim 8-10 to carry out enrichment to the biological particles in biological specimen, and it comprises the following steps:
Sleeve pipe is assemblied in centrifuge container, and opens the shifting board of sleeve pipe;
Density medium is loaded in centrifuge container body, and close the shifting board of sleeve pipe;
By in biological specimen sprue bushing;
Open the shifting board of sleeve pipe, biological specimen is contacted with density medium, carry out centrifugal treating, after centrifugal treating, above the shifting board of sleeve pipe, form biological particles enriched layer;
Close the shifting board of sleeve pipe, sleeve pipe is taken out from centrifuge container body, biological particles enriched layer is shifted, completes the enrichment of biological particles in biological specimen;
Wherein, described biological specimen is blood or tissue samples.
12. methods according to claim 11, wherein, the upper surface loading the density medium in centrifuge container body contacts with the bottom face of described sleeve pipe or a little more than bottom face.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210518450.0A CN103849562B (en) | 2012-12-05 | 2012-12-05 | Sleeve for biological sample in-vitro purification treatment |
| PCT/CN2013/088668 WO2014086302A1 (en) | 2012-12-05 | 2013-12-05 | Casing tube used for purifying and treating biological samples in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210518450.0A CN103849562B (en) | 2012-12-05 | 2012-12-05 | Sleeve for biological sample in-vitro purification treatment |
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| Publication Number | Publication Date |
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| CN103849562A CN103849562A (en) | 2014-06-11 |
| CN103849562B true CN103849562B (en) | 2015-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210518450.0A Active CN103849562B (en) | 2012-12-05 | 2012-12-05 | Sleeve for biological sample in-vitro purification treatment |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104031831B (en) * | 2014-07-01 | 2019-10-15 | 北京圣浦博大生物科技有限公司 | A kind of separation method of haemocyte separating pipe and mononuclearcell |
| CN114273091B (en) * | 2021-12-30 | 2022-07-08 | 青岛市中心血站 | Blood rapid centrifugal separation and collection device and control method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201825948U (en) * | 2010-09-30 | 2011-05-11 | 何洪敏 | Combined centrifuge tube for preparing cell blocks by using cast-off cells |
| CN102321582A (en) * | 2011-08-29 | 2012-01-18 | 深圳市中美康士生物科技有限公司 | Granulocyte separating medium, and method for granulocyte separating and activity detecting |
| CN202359118U (en) * | 2011-12-01 | 2012-08-01 | 石国清 | Gradient separation device of cast-off cells |
| WO2012154257A1 (en) * | 2011-02-17 | 2012-11-15 | Prometheus Laboratories Inc. | Apparatus and method for isolating leukocytes and tumor cells by filtration |
| CN202925017U (en) * | 2012-12-05 | 2013-05-08 | 北京东方华辉生物医药科技有限公司 | Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples |
-
2012
- 2012-12-05 CN CN201210518450.0A patent/CN103849562B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201825948U (en) * | 2010-09-30 | 2011-05-11 | 何洪敏 | Combined centrifuge tube for preparing cell blocks by using cast-off cells |
| WO2012154257A1 (en) * | 2011-02-17 | 2012-11-15 | Prometheus Laboratories Inc. | Apparatus and method for isolating leukocytes and tumor cells by filtration |
| CN102321582A (en) * | 2011-08-29 | 2012-01-18 | 深圳市中美康士生物科技有限公司 | Granulocyte separating medium, and method for granulocyte separating and activity detecting |
| CN202359118U (en) * | 2011-12-01 | 2012-08-01 | 石国清 | Gradient separation device of cast-off cells |
| CN202925017U (en) * | 2012-12-05 | 2013-05-08 | 北京东方华辉生物医药科技有限公司 | Sleeve tube and centrifugation container for in-vitro purifying treatment of biological samples |
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| CN103849562A (en) | 2014-06-11 |
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Application publication date: 20140611 Assignee: Zhongguancun Technology Leasing Co.,Ltd. Assignor: BEIJING DONGFANG HUAHUI BIOMEDICAL TECHNOLOGY Co.,Ltd. Contract record no.: X2023980044727 Denomination of invention: A sleeve for in vitro purification of biological samples Granted publication date: 20150610 License type: Exclusive License Record date: 20231026 |
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Denomination of invention: A sleeve for in vitro purification of biological samples Effective date of registration: 20231027 Granted publication date: 20150610 Pledgee: Zhongguancun Technology Leasing Co.,Ltd. Pledgor: BEIJING DONGFANG HUAHUI BIOMEDICAL TECHNOLOGY Co.,Ltd. Registration number: Y2023980063147 |
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